CN102565063A - Reagent detection device, system, method, blood analysis device - Google Patents
Reagent detection device, system, method, blood analysis device Download PDFInfo
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- CN102565063A CN102565063A CN2010106196116A CN201010619611A CN102565063A CN 102565063 A CN102565063 A CN 102565063A CN 2010106196116 A CN2010106196116 A CN 2010106196116A CN 201010619611 A CN201010619611 A CN 201010619611A CN 102565063 A CN102565063 A CN 102565063A
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Abstract
The invention discloses a reagent detection device, which comprises a light emitting unit, a reagent pipeline and a light receiving unit, wherein the light emitting unit is used for emitting an incident light signal, the reagent pipeline is composed of light-transmitting materials, the inner wall of the reagent pipeline is enclosed into a reagent cavity for reagent to flow by, incident light and the surface normal of the reagent pipeline the incident light corresponds to form an incident angle larger than a critical angle, and the light receiving unit is used for receiving an emergent light signal emitted from inside of the reagent pipeline. By enabling the incident angle formed by the incident light and the surface normal of the reagent pipeline which the incident light corresponds to to be larger than the critical angle and by utilizing the light signal received by the light receiving unit, the reagent detection device can accurately detect whether the reagent exists in the reagent pipeline so as to ensure the reliability of a measurement result.
Description
Technical field
The present invention relates to blood analysis device, especially reagent pick-up unit and method.
Background technology
Blood analysis device uses the reagent that sucks fixed amount in the outer reagent bucket of power source slaves such as syringe, negative pressure source, fixed displacement pump, joins in the container that reacts with blood sample.Reagent is divided into kinds such as dilution, hemolytic agent, coloring agent, respectively blood sample is diluted, dissolves, dyes.
After blood sample and the reagent effect, could form can be to be detected liquid.As detect erythrocyte number in the blood sample, and need to use dilution that the blood sample sample is diluted, could count red blood cell in the sample liquid then, and then calculate the erythrocyte parameter of blood sample; Detect the hemoglobin parameters of blood sample, need to use hemolytic agent that the haemoglobin in the red blood cell is discharged, could calculate the hemoglobin parameters of blood sample then according to the transmittance of sample liquid.
The inaccurate meeting of blood analysis device reagent dosage causes the processing imperfection of blood sample; Parameter measurement is inaccurate; Therefore must the reagent that get into instrument be detected, find in time that reagent uses up, various types of faults such as bubble are arranged in the reagent, guarantee the reliability of measurement result.
Whether prior art is through having enough reagent in the outside reagent bucket of float sensor detecting instrument, and when amount of reagent was reduced to a certain degree, float sensor was uploaded no reagent signal, and blood analysis device no longer sucks reagent, the reagent that prompting more renews.This method can not detect the pipeline from the reagent bucket to machine intimate and have or not reagent, can not find in the pipeline bubble of separating out because of reasons such as time of repose are long, influences the reliability of reagent dosage; When float sensor was uploaded no reagent signal, also residual a certain amount of reagent caused waste in the reagent bucket.
Summary of the invention
The technical problem underlying that the present invention solves is, a kind of reagent pick-up unit is provided, and can have or not reagent in the detectable pipeline.
According to an aspect of the present invention, a kind of reagent pick-up unit is provided, comprises: Optical Transmit Unit is used to send incident optical signal; The reagent pipeline, this reagent pipeline is made up of light transmissive material, and the inwall of said reagent pipeline surrounds a reagent chamber, is used to supply reagent to flow through, and the incident angle of said incident light and its reagent corresponding pipeline surface normal formation is more than or equal to critical angle; Light receiving unit is used for receiving the emergent light signal from the outgoing of reagent pipeline.
According to a further aspect of the invention, a kind of reagent detection system is provided, comprises above-mentioned reagent pick-up unit, also comprise: reagent container is used to hold reagent; Reaction vessel is used to hold the mixed liquor of sample and reagent; Propulsion system are used for the power of drawing reagent from reagent container is provided, and said reagent container is connected through liquid line with said reaction vessel, connect above-mentioned reagent pick-up unit on the said liquid line.
According to another aspect of the invention, a kind of blood analysis device is provided, comprise above-mentioned reagent detection system.
According to a further aspect of the present invention, a kind of reagent test method is provided, the step of said reagent test method comprises: Optical Transmit Unit is provided, sends incident optical signal; The reagent pipeline is provided, and this reagent pipeline is made up of light transmissive material, and the inwall of said reagent pipeline surrounds a reagent chamber, is used to supply reagent to flow through, and the incident angle of said incident light and its reagent corresponding pipeline surface normal formation is more than or equal to critical angle; Light receiving unit is provided; The emergent light signal of reception outgoing from the reagent pipeline, as reagent during through said reagent pipeline, said photo-detector receives/less than light signal; When no reagent during through said reagent pipeline, said photo-detector does not receive/arrives light signal.
According to a further aspect of the present invention, a kind of blood analysis device reagent test method is provided, the step of said blood analysis device reagent test method comprises: draw step, from reagent container, draw reagent; Detect step, through whether having reagent in the reagent pick-up unit detectable pipeline; Drain steps enters the reagent that is drawn in the reaction vessel.
The present invention is provided with incident angle that incident light and its reagent corresponding pipeline surface normal form more than or equal to critical angle through employing; The light signal that receives through light receiving unit; Whether there is reagent in the detectable pipeline more accurately, guaranteed the reliability of measurement result.
Description of drawings
Fig. 1 is the synoptic diagram of reagent pick-up unit of the present invention;
Fig. 2 is a light receiving unit position view in a kind of embodiment of reagent pick-up unit of the present invention;
Fig. 3 is a kind of synoptic diagram of light receiving unit in a kind of embodiment of reagent pick-up unit of the present invention;
Fig. 4 is the another kind of synoptic diagram of light receiving unit in a kind of embodiment of reagent pick-up unit of the present invention;
Fig. 5 is another synoptic diagram of light receiving unit in a kind of embodiment of reagent pick-up unit of the present invention;
Fig. 6 is the imbibition stage synoptic diagram of reagent detection system of the present invention;
Fig. 7 is the blowdown period synoptic diagram of reagent detection system of the present invention;
Fig. 8 is the synoptic diagram that has the reagent detection system of liquid storage tank of the present invention;
Fig. 9 is a kind of embodiment process flow diagram of blood analysis device reagent test method of the present invention;
Figure 10 is the another kind of embodiment process flow diagram of blood analysis device reagent test method of the present invention;
Figure 11 is the process flow diagram that has the blood analysis device reagent test method of liquid storage step of the present invention.
Embodiment
Combine accompanying drawing that the present invention is done further explain through embodiment below.
The invention discloses a kind of reagent pick-up unit, this pick-up unit comprises: Optical Transmit Unit is used to send incident optical signal; The reagent pipeline, this reagent pipeline is made up of light transmissive material, and the inwall of said reagent pipeline surrounds a reagent chamber, is used to supply reagent to flow through, and the incident angle that said incident light and reagent pipeline surface normal form is more than or equal to critical angle; Light receiving unit is used for receiving the emergent light signal that transmits from the reagent pipeline.
The present invention is through the shape of design reagent cavity; Make the surperficial incident angle of finding to form of incident light and reagent more than or equal to critical angle; The difference of the transmittance that receives according to light receiving element judges whether the reagent pipeline exists reagent; Improve the reliability that detects, can not cause the waste of reagent again.
Specifically please refer to Fig. 1, Optical Transmit Unit 100 is used to send incident optical signal, the Optical Transmit Unit 100 preferred pointolites that adopt; For example; Can be laser, can also be LED, the optical system of also forming with a plurality of optical elements certainly; For example, increase optical filter, frosted glass and aperture in the place ahead of expansion light source (like halogen tungsten lamp) and form pointolite.Reagent pipeline 200 is made up of light transmissive material, and the inwall of reagent pipeline 200 surrounds a reagent chamber 210, is used for reagent and passes through.Light receiving unit 300 receives from Optical Transmit Unit 100 and sends and see through the emergent light signal behind the reagent pipeline 200.Light receiving unit 300 can be independent photo-detector, a kind of preferred embodiment in, Optical Transmit Unit 100 adopts the optocoupler that is packaged together to detect with light receiving element 300.
The surface of reagent chamber comprises first surface and second surface, and first surface is meant the surface that incident light shines earlier, and first surface is the plane, and incides incident angle that the light of first surface forms more than or equal to critical angle.In one embodiment, as shown in Figure 1, first surface 212 is surfaces that incident ray shines earlier, and second surface 214 is the surfaces that shine with the corresponding transmitted light of first surface.Light receiving unit 300 is placed with Optical Transmit Unit 100 relatively, is used to receive the emergent light signal.When there was reagent the illumination position of reagent pipeline 200, transmission took place in incident light on first surface 212, and light receiving unit 300 receives the emergent light signal of incident light in first surface 212 transmissions; When the illumination position of reagent pipeline 200 did not have reagent, total reflection took place in incident light on first surface 212, and light receiving unit 300 does not receive light signal.In another embodiment, as shown in Figure 2, light receiving unit 302 and Optical Transmit Unit 100 vertical placements are used to receive the emergent light signal.When there was reagent the illumination position in the reagent pipeline 200, transmission took place in incident light on first surface 212, and light receiving element 302 does not receive light signal; When the illumination position of reagent pipeline 200 did not have reagent, total reflection took place at first surface 212 in incident light, and light receiving unit 302 receives the emergent light signal of incident light in first surface 212 reflections.On the basis of above-mentioned embodiment; As shown in Figure 3; Two light receiving units 300,302 are arranged; Light receiving unit 300 is placed with Optical Transmit Unit 100 relatively, and light receiving unit 302 and Optical Transmit Unit 100 vertical placements promptly are captured in the outgoing signal of the transmission on the first surface 212 and the emergent light signal of reflection simultaneously.If collect the emergent light signal of reflection, then detecting in the reagent pipeline 200 does not have reagent; If collect the emergent light signal of transmission, then detecting in the reagent pipeline 200 has reagent; Perhaps the emergent light signal of reflection is stronger; Then detect in the reagent pipeline 200 and do not have reagent; If it is stronger to collect the emergent light signal of transmission; Then detecting in the reagent pipeline 200 has reagent, utilizes the difference of the light signal that receives of light receiving unit 300 and light receiving unit 302, and then judges whether there is reagent in the reagent pipeline 200.
The mentioned reagent pick-up unit is mainly according to total reflection principle; Total reflection principle is: light is that the optically denser medium directive refractive index of n1 is when being the optically thinner medium of n2 by refractive index; As incident angle i during greater than critical angle=arcsin (n2/n1), incident ray generation total reflection, refracted ray disappears.The ultimate principle that reagent of the present invention detects is: the illumination of sending when Optical Transmit Unit is mapped to the first surface of reagent pipeline, if in the reagent pipeline, have reagent, light receiving unit receives/less than light signal; If in the reagent pipeline, there is not reagent, shine the light generation total reflection of first surface, light receiving unit does not receive/arrives light signal.
For guaranteeing the realization of above-mentioned detection principle, as shown in Figure 1, at first, reagent pipeline 200 adopts light transmissive material, to guarantee that light can see through reagent pipeline 200 and shine on the first surface 212; Simultaneously, reagent pipeline 200 is also wanted the relatively big material of selective refraction rate, to satisfy the necessary condition that total reflection takes place, promptly incides optically thinner medium from optically denser medium.For example, when reagent pipeline 200 is sky, when just reagent pipeline 200 does not have reagent, in the reagent pipeline air, thus the material that reagent pipeline 200 adopts greater than air refraction, such as glass, plastics etc.In addition, for realizing total reflection, also will satisfy incident angle greater than critical angle, so the shape of the first surface 212 of reagent chamber 210 is also extremely important.
In a kind of concrete embodiment, the refractive index of reagent piping material is about 1.4, and the refractive index of air is 1, and critical angle=arcsin (n2/n1)=arcsin (1/1.4)=45 °, calculate, incident angle is during more than or equal to 45 °, and total reflection can take place in light.
In one embodiment, as shown in Figure 4, first surface 212 is the plane, vertically reagent pipeline 200 is cut open along the optical axis L direction, obtains a sectional view of reagent pipeline 200.On this section, then can know according to geometric relationship, incide in the incident angle of first surface 212 light formation; α is minimum; If satisfy α >=critical angle, total reflection all can take place all greater than critical angle in the incident angle that shines the light formation of other positions on reagent chamber 200 surfaces.
In another embodiment, as shown in Figure 5, the horizontal plane at optical axis L place is divided into two parts up and down with reagent chamber 210, and correspondingly, first surface 212 is divided into first surface 216 and following first surface 218.Last first surface 216 is the plane with following first surface 218, and goes up first surface 216 and following first surface 218 formation one angle theta.According to top embodiment, incide that the light of first surface 216 forms incident angle in, γ 1 minimum; Incide down in the incident angle that the light of first surface 218 forms γ 2 minimums.Can know according to geometric relationship,, total reflection take place all if γ 1 >=critical angle then shines incident angle that the light of first surface forms all more than or equal to critical angle; If γ 2 >=critical angle then shines down incident angle that the light of first surface forms all more than or equal to critical angle, total reflection all takes place.Thus, if γ 1 >=critical angle, and γ 2 >=critical angle then shines incident angle that the light of reagent chamber 200 lip-deep other positions forms all greater than critical angle.According to geometric relationship, can calculate θ.For example, in a kind of concrete embodiment, if γ 1=critical angle=45 °, γ 2=critical angle=45 ° can calculate θ, the distance dependent of θ and reagent chamber and light source.
On the basis of above-mentioned embodiment; Can reagent chamber be divided into two parts up and down with any horizontal plane; Obtain first surface and following first surface; And the incident angle that the light that incides first surface forms is more than or equal to critical angle, and the incident angle of light formation that incides down first surface is more than or equal to critical angle.
On the basis of above-mentioned embodiment, can also reagent chamber be divided into a plurality of surfaces with arbitrary plane, such as three surfaces, four surfaces etc.The length of each part can be identical, also can be inequality.As long as the incident angle that the light of incident forms is more than or equal to critical angle.The surface that is divided into is many more, and first surface levels off to curved surface more, if unrestricted the division will obtain a curved surface.Adopt above-mentioned this structure, during no reagent, first surface makes photocell send all light generation total reflections in pipeline, and the transmittance of reagent pipeline is 0; When in the pipeline reagent being arranged,, survey energy also greater than the fixing structure in pitch angle because of curved surface has the effect of convergence to light.
According to above-mentioned detection principle, if bubble is arranged, then total reflection can take place on first surface, equally do not receive light signal yet.Can judge further that according to the width of level negative pulse the reason of total reflection is that reagent chamber has bubble or no reagent, for example: when bubble is arranged, be a narrow negative pulse; When not having reagent, the width of pulse is continuously low.
This signal piping is used for blood analysis device, is used for the detectable device and whether has reagent, and the reagent in the reagent pipeline can be dilution, also can be clean-out system, can also be other reagent such as hemolytic agent.
Those of ordinary skill in the art can know, the light that incides first surface possibly not have yet total reflection takes place fully, also transmission possibly take place partly, can judge whether to exist reagent according to the ratio of total reflection.Those of ordinary skill in the art can know that also first surface not necessarily all is the workspace, and promptly not all reflection of light light and/or transmitted light that shines first surface received by light receiving element.First surface can also be regarded the workspace as in the present invention, also should belong within the scope of the present invention.
The present invention also provides a kind of reagent detection system, comprises reagent container, is used to hold reagent; Reaction vessel is used to hold the mixed liquor of sample and reagent; Propulsion system are used for the power of drawing reagent from reagent container is provided, and said reagent container is connected through liquid line with said reaction vessel, connect above-mentioned reagent pick-up unit on the said liquid line.Propulsion system can be connected on the liquid line of reagent container and reaction vessel, in the middle of the reagent sucting reaction container in the reagent container.
In a kind of concrete embodiment; On the liquid line of connection reagent pick-up unit and said reaction vessel T-valve is set; Said T-valve connects said reagent container, said reaction vessel and said propulsion system respectively, and said propulsion system also are used for controlling the amount of reagent of drawing from reagent container.As shown in Figure 6, this reagent detection system comprises reagent pick-up unit 102, reagent container 104, and reaction vessel 106, propulsion system 108 are used to provide suction reagent needed positive negative pressure; Also comprise T-valve 110, be connected with reagent container 104, reaction vessel 106, propulsion system 108 respectively, and accomplish the action of suction reagent according to sequential.For example, open T-valve 110, blood analysis device is drawn reagent from reagent container 104, and propulsion system can be syringe, fixed displacement pump etc.Reagent pipeline 200, begin to flow to absorption from reagent and accomplish, reagent stops in the process of flowing in the reagent pipeline 200, through whether be full of reagent in the reagent pick-up unit 102 detectable pipelines always.As shown in Figure 7, after reagent was drawn in completion, switch three-way valve 110 entered reagent in the reaction vessel 106, mixes with blood sample.
In another embodiment, also comprise liquid storage tank in the liquid line of connection reagent pick-up unit and reaction vessel, be used for the temporary reagent of drawing from reagent container, on the liquid line that connects said liquid storage tank and said reaction vessel, valve is set.As shown in Figure 8, in the liquid line 103 between connection reagent pick-up unit 102 and the reaction vessel 104 liquid storage tank 105 is set, through propulsion system the reagent in the reagent container is temporarily sucked in the liquid storage tank 105, to avoid in reaction vessel, adding bubble.Be provided with simultaneously and reserve in the liquid storage tank, in the amount of reagent that guarantees in satisfying proper testing, to use, in liquid storage tank, leave certain surplus and satisfy test next time greater than amount of reagent when Pretesting.Through the temporary effect of above-mentioned liquid storage tank, if the reagent that the reagent pick-up unit detects in the reagent container is finished using, when the prompting user changes reagent, still can accomplish and work as Pretesting, do not influence test speed.
On the basis of above-mentioned embodiment, on the liquid line that connects said liquid storage tank 105 and said reaction vessel 104, valve 107 is set, open when needing reaction.
In another embodiment, on the liquid line that connects reagent pick-up unit 102 and liquid storage tank 105, valve 109 is set, opens when from the outside agent bucket, sucking reagent.
The present invention also provides a kind of blood analysis device; Comprise above-mentioned reagent detection system; Through the reagent signal piping is set in the pipeline between reagent container and reagent container; Use photocell irradiation reagent signal piping, utilize the difference of transmittance when reagent and no reagent are arranged, judge that in the reagent pipeline be that reagent is arranged.Owing to adopt the first surface of above-mentioned special shape, during no reagent, incident light is in first surface generation total reflection; When in the pipeline reagent being arranged, incident light is in first surface generation transmission.
When blood analysis device sucked reagent, blood analysis device carried out integration to the time of reagent pipeline generation total reflection, calculates the accumulated time of bubble in the pipeline with this, according to the requirement of instrument reagent addition degree of accuracy, judged whether the reagent that sucks meets the demands.
The very big dilution of consumption during for detection; Earlier fast be drawn in liquid storage tank with negative pressure the dilution in the reagent container; Monitor the liquid line between reagent container and the liquid storage tank in real time; When the ratio of finding to take place in the liquid line total reflection went beyond the limit, the liquid line between closing at once from the reagent container to the liquid storage tank was accomplished when time measuring and pointing out the user to change dilution with the dilution in the liquid storage tank.This method had both satisfied the requirement of detection speed, again can reagent is not inconsistent and the blood sample that requires to cause to have begun to detect can not be accomplished test because of sucking.
In one embodiment, limited if light source and detector have been packaged together because of the inner space of blood analysis device, too big assembly can not be installed.The distance of light source and photo-detector can not be too far away, so reagent pipeline external dimensions has restriction.Require cross section of fluid channel long-pending big as far as possible putting before this, the guarantee reagent pipeline does not influence the flow velocity of reagent.
For embodiment of the present invention can be made much of, provide a lot of concrete details to describe and specify.But those skilled in the art can recognize that wherein certain or some concrete details are omissible, perhaps also can use other method, element or material.In some cases, some operation steps is not described in detail.
The present invention also provides a kind of blood analysis device reagent test method, comprises step: draw step, from reagent container, draw reagent; Detect step, through whether having reagent in the pick-up unit detectable pipeline; Drain steps enters the reagent that is drawn in the reaction vessel.As shown in Figure 9, be the process flow diagram of reagent test method of the present invention, mainly comprise and draw step 500, detection step 502 and drain steps 504.Wherein in detecting step 502, detect with above-mentioned reagent pick-up unit.
In a kind of concrete embodiment, shown in figure 10, reagent test method also comprises quantitative step 506, and quantitative reagent is drawn in control from reagent container; Imbibition is accomplished, and carries out switch step 510, will from reagent container, imbibition switch to discharge opeing in reaction vessel, if do not accomplish imbibition, continues to carry out absorption step 500.
In another embodiment,, also comprise the liquid storage step, be used for the temporary reagent of drawing from reagent container for avoiding bubble and reagent mix.Shown in figure 11, the blood analysis device reagent test method also comprises liquid storage step 508, is used for the temporary liquid of drawing from reagent container.
As shown in Figure 8, in the liquid line 103 between connection reagent pick-up unit 102 and the reaction vessel 104 liquid storage tank 105 is set, through propulsion system the reagent in the reagent container is temporarily sucked in the liquid storage tank 105, to avoid in reaction vessel, adding bubble.Be provided with simultaneously and reserve in the liquid storage tank, in the amount of reagent that guarantees in satisfying proper testing, to use, in liquid storage tank, leave certain surplus and satisfy test next time greater than amount of reagent when Pretesting.Through the temporary effect of above-mentioned liquid storage tank, if the reagent that the reagent pick-up unit detects in the reagent container is finished using, when the prompting user changes reagent, still can accomplish and work as Pretesting, do not influence test speed.
In a kind of concrete embodiment.In whole suction process, calculate the ratio of bubble, allowing under the consistent prerequisite of bubble ratio, reagent be empty ratio when surpassing the limit of presetting, and closes the reagent pipeline to the pipeline between the liquid storage tank, makes bubble can not get into liquid storage tank.But when the discontented foot of bubble ratio requires in finding pipeline, possibly there is the higher reagent of air bubble content to be used to the dilution of sample, influenced measuring reliability.Owing to inevitably there are some small bubbles in the reagent, if will allow the low excessively of bubble ratio setting, can cause unnecessary warning too frequent again, influence the operate as normal of machine.
For overcoming above-mentioned problem, shown in as shown in the figure 8, suppose that reagent pick-up unit 102 is that V, the reagent flow velocity in pipeline is S to the sebific duct volume between the liquid storage tank 105, the timeslice length of design monitoring is T, requires T＜V/S.In detecting step 502, the signal according to detector is uploaded calculates the ratio that reagent is empty (no reagent) in timeslice.In liquid storage step 508, if find T in certain timeslice, when reagent be empty ratio less than preset limit, the reagent that will from reagent, be drawn to was kept in liquid storage tank.If find T in certain timeslice; Reagent is that empty ratio is when surpassing preset limit; Close reagent pick-up unit 102 to the liquid line between the liquid storage tank 105; Make bubble can not get into liquid storage tank, utilize the amount of reagent of liquid storage tank 105 stored to accomplish after inferior measurement, require the user to change the test of carrying out follow-up blood sample behind the dilution again.
On the basis of above-mentioned embodiment, be used to the dilution of sample for fear of the higher reagent of air bubble content, in the process of drawing reagent; The signal of uploading according to detector; Calculating reagent in timeslice be empty ratio, finds T in certain timeslice, when reagent be empty ratio above the limit of presetting; Close the reagent pipeline to the pipeline between the liquid storage tank; Make bubble can not get into liquid storage tank, utilize the amount of reagent of liquid storage tank stored to accomplish after inferior measurement, require the user to change the test of carrying out follow-up blood sample behind the dilution again.
Adopt this mode, the reagent in the liquid storage tank does not contain bubble, can guarantee the requirement of testing; The action of in machine, inhaling appearance from the reagent bucket is continuous, quick, inhales appearance like negative pressure.
Technical characterictic of describing in the embodiment of the invention or operation steps can make up in any suitable manner.Those skilled in the art understand easily, and the step in the method that the embodiment of the invention is described or the order of action can change.Therefore, require certain sequence except as otherwise noted, any order in accompanying drawing or detailed description is just for illustrative purpose, rather than necessary order.
More than describe the present invention through concrete embodiment, but the present invention is not limited to these concrete embodiment.It will be understood by those skilled in the art that and to make various modifications to the present invention, be equal to replacement, change or the like that these conversion all should be within protection scope of the present invention as long as do not deviate from spirit of the present invention.In addition, above many places described " embodiment " or " another embodiment " etc. represent various embodiment, can certainly be with its all or part of being combined among the embodiment.
More than describe the present invention through concrete embodiment, but the present invention is not limited to these concrete embodiment.It will be understood by those skilled in the art that and to make various modifications to the present invention, be equal to replacement, change or the like that these conversion all should be within protection scope of the present invention as long as do not deviate from spirit of the present invention.
Claims (12)
1. reagent pick-up unit is characterized in that: comprising:
Optical Transmit Unit is used to send incident optical signal;
The reagent pipeline, this reagent pipeline is made up of light transmissive material, and the inwall of said reagent pipeline surrounds a reagent chamber, is used to supply reagent to flow through, and the incident angle of said incident light and its reagent corresponding pipeline surface normal formation is more than or equal to critical angle;
Light receiving unit is used for receiving the emergent light signal from the outgoing of reagent pipeline.
2. reagent pick-up unit according to claim 1; It is characterized in that: the surface of said reagent chamber comprises first surface; Said first surface is the surface that incident ray shines earlier, and said first surface is the plane, and incides incident angle that the light of first surface forms more than or equal to critical angle.
3. reagent pick-up unit according to claim 1; It is characterized in that: the surface of said reagent chamber comprises first surface; Said first surface comprises first surface and following first surface; The said first surface of going up is the plane with following first surface, and the incident angle of light formation that incides first surface is more than or equal to critical angle, and the incident angle of light formation that incides down first surface is more than or equal to critical angle.
4. according to the said reagent pick-up unit of claim 1, described Optical Transmit Unit and described light receiving unit are placed around said reagent signal piping relatively, or vertical the placement.
5. reagent detection system is characterized in that: comprise each described reagent pick-up unit in the claim 1 to 4, also comprise:
Reagent container is used to hold reagent;
Reaction vessel is used to hold the mixed liquor of sample and reagent;
Propulsion system are used for providing the power of drawing reagent from reagent container,
Said reagent container is connected through liquid line with said reaction vessel, connects above-mentioned reagent pick-up unit on the said liquid line.
6. reagent detection system according to claim 5; It is characterized in that: on the liquid line of said connection reagent pick-up unit and said reaction vessel T-valve is set; Said T-valve connects said reagent container, said reaction vessel and said propulsion system respectively, and said propulsion system also are used for controlling the amount of reagent of drawing from reagent container.
7. reagent detection system according to claim 5; It is characterized in that: also comprise liquid storage tank in the liquid line of said connection reagent pick-up unit and said reaction vessel; Be used for the temporary reagent of drawing from reagent container, on the liquid line that connects said liquid storage tank and said reaction vessel, valve be set.
8. a blood analysis device is characterized in that: comprise each described reagent detection system in the claim 5 to 7.
9. reagent test method, it is characterized in that: the step of said reagent test method comprises:
Optical Transmit Unit is provided, sends incident optical signal;
The reagent pipeline is provided, and this reagent pipeline is made up of light transmissive material, and the inwall of said reagent pipeline surrounds a reagent chamber, is used to supply reagent to flow through, and the incident angle of said incident light and its reagent corresponding pipeline surface normal formation is more than or equal to critical angle;
Light receiving unit is provided, receives the emergent light signal of outgoing from the reagent pipeline,
As reagent during through said reagent pipeline, said photo-detector receives/less than light signal,
When no reagent during through said reagent pipeline, said photo-detector does not receive/arrives light signal.
10. blood analysis device reagent test method, it is characterized in that: the step of said blood analysis device reagent test method comprises:
Draw step, from reagent container, draw reagent;
Detect step, through whether having reagent in the reagent pick-up unit detectable pipeline;
Drain steps enters the reagent that is drawn in the reaction vessel.
11. blood analysis device reagent test method according to claim 10 is characterized in that, the step of said blood analysis device reagent test method also comprises:
Quantitatively step is controlled and from reagent container, is drawn quantitative reagent;
Switch step, imbibition switches to discharge opeing in reaction vessel from reagent container, and the quantitative reagent that will from reagent container, be drawn to is discharged in the reaction vessel.
12. blood analysis device reagent test method according to claim 10 is characterized in that: the step of said blood analysis device reagent test method also comprises:
The liquid storage step, the reagent that will from reagent container, be drawn to is temporary in liquid storage tank.
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| CN107687963A (en) * | 2016-08-04 | 2018-02-13 | 深圳迈瑞生物医疗电子股份有限公司 | Confirmatory sample samples the method, apparatus and blood cell analyzer of reliability |
| WO2019000459A1 (en) * | 2017-06-30 | 2019-01-03 | 深圳迈瑞生物医疗电子股份有限公司 | Liquid-supply device, sample-analyzing apparatus and liquid-supply method |
| WO2019127562A1 (en) * | 2017-12-30 | 2019-07-04 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analyzer and reagent supply method therefor |
| CN113030020A (en) * | 2019-12-24 | 2021-06-25 | 北汽福田汽车股份有限公司 | Gas detection device in oil supply pipeline and vehicle with same |
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| CN113130051A (en) * | 2019-12-31 | 2021-07-16 | 科美诊断技术股份有限公司 | Reagent amount management method and device |
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