CN111024481A - Sample mixing method and sample analyzer - Google Patents
Sample mixing method and sample analyzer Download PDFInfo
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- CN111024481A CN111024481A CN201911398107.5A CN201911398107A CN111024481A CN 111024481 A CN111024481 A CN 111024481A CN 201911398107 A CN201911398107 A CN 201911398107A CN 111024481 A CN111024481 A CN 111024481A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
- G01N2001/387—Other diluting or mixing processes mixing by blowing a gas, bubbling
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Abstract
The invention discloses a sample mixing method and a sample analyzer, wherein the sample mixing method comprises the following steps: moving the sampling needle to a first position, and controlling the pump to enable the sampling needle to suck first liquid from the first position; moving the sampling needle to the reaction tank, and controlling the pump to enable the sampling needle to inject first liquid into the reaction tank; moving the sampling needle to a second position, and controlling the pump to enable the sampling needle to suck second liquid from the second position; moving the sampling needle to the reaction tank, and controlling the pump to enable the sampling needle to inject second liquid into the reaction tank; moving the sampling needle to a position above the liquid level of the reaction tank, and controlling the pump to enable the sampling needle to suck air; and moving the sampling needle to a position below the liquid level of the reaction tank, and controlling the pump to enable the sampling needle to inject air into the liquid in the reaction tank. The sample blending method provided by the invention can effectively improve the preparation mixing efficiency, can finish sampling and mixing work by using the same pump and the same sampling needle, has low requirement on a system, is beneficial to equipment simplification and reduces the cost.
Description
Technical Field
The invention relates to the field of biological pharmacy, in particular to a sample blending method and a sample analyzer.
Background
When the sample of the detection instrument is prepared, a sample sucking needle is required to suck and distribute a trace amount of blood sample into a reaction tank filled with a substrate solution, and a certain uniform mixing mode is adopted to fully mix sample mixed liquid in the reaction tank so as to facilitate the subsequent uniform sample liquid to be used as a pre-diluted sample to participate in secondary distribution or be directly used as a liquid to be detected.
At present, because of the influence of latex-antibody self physical characteristics, use consumption and the like, the common analyzer for combined detection of blood cells and proteins has small mixing liquid amount and large viscosity, most of the analyzers adopt a suction and spitting mixing mode, and although the mixing mode can fully mix latex-antibody sample liquid, the analyzer still has the following problems:
firstly, dilution liquid can be caused to dilute and mix sample liquid in the mixing process, because the sample mixing preparation place, namely the reaction tank is connected with the measuring tank through a liquid pipe, the measuring tank is connected with the injector, and the liquid pipe and the measuring tank are filled with the dilution liquid, when the injector pumps, the sample liquid in the reaction tank can be pumped to the measuring tank through the liquid pipe, so that the sample liquid can not be contacted with the dilution liquid, meanwhile, the inner diameter of the measuring tank is generally far larger than the diameter of the liquid pipe, and the sample liquid entering the measuring tank flows with the dilution liquid in a mixing manner and can also dilute the sample liquid;
secondly, in the process of repeatedly pumping and uniformly mixing, the protein in the sample liquid is more easily adhered to the inner walls of the liquid pipe and the measuring pool, so that the cleaning difficulty is greatly increased.
Therefore, how to effectively simplify the sample blending method and improve the detection precision is a technical problem that needs to be solved by the technical personnel in the field at present.
Disclosure of Invention
The invention aims to provide a sample blending method and a sample analyzer, which can reduce the structural requirement on a system and improve the preparation and mixing efficiency of a sample.
In order to achieve the purpose, the invention provides the following technical scheme:
a sample blending method is applied to a blending system which comprises a sampling needle, a pump, a pipeline for connecting the sampling needle and the pump, a moving device for driving the sampling needle to move and a reaction tank; the method comprises the following steps:
moving the sampling needle to a first position, and controlling the pump to enable the sampling needle to suck a first liquid from the first position;
moving the sampling needle to the reaction tank, and controlling the pump to enable the sampling needle to inject first liquid into the reaction tank;
moving the sampling needle to a second position, and controlling the pump to enable the sampling needle to suck a second liquid from the second position;
moving the sampling needle to the reaction tank, and controlling the pump to enable the sampling needle to inject a second liquid into the reaction tank;
moving the sampling needle to be above the liquid level of the reaction tank, and controlling the pump to enable the sampling needle to suck air;
and moving the sampling needle to a position below the liquid level of the reaction tank, and controlling the pump to enable the sampling needle to inject air into the liquid in the reaction tank.
Preferably, the pump is a syringe pump, and a cleaning solution containing cavity is arranged in the syringe pump;
after the controlling the pump to make the sampling needle inject the first liquid into the reaction cell, the method further includes:
controlling the pump to enable the cleaning liquid in the cleaning liquid containing cavity to flow out along the pipeline and the sampling needle;
and air-drying the outer surface of the sampling needle.
Preferably, after controlling the pump to inject the second liquid into the reaction cell by the sampling needle, the method further includes:
controlling the pump to enable the cleaning liquid in the cleaning liquid containing cavity to flow out along the pipeline and the sampling needle;
and air-drying the outer surface of the sampling needle.
Preferably, before moving the sampling needle to the first position and controlling the pump to make the sampling needle suck the first liquid from the first position, the method further includes: controlling the pump to enable the sampling needle to suck an air column;
before moving the sampling needle to the second position and controlling the pump to make the sampling needle suck the second liquid from the second position, the method further comprises: and controlling the pump to enable the sampling needle to suck the air column.
Preferably, the moving means includes a sampling needle swinging part;
the moving the sampling needle below the liquid level of the reaction tank further comprises:
and controlling the sampling needle to swing.
Preferably, after the sampling needle is moved to a position below the liquid level in the reaction cell, the pump is controlled to inject air into the liquid in the reaction cell, and the sampling needle is simultaneously controlled to swing.
Preferably, the moving the sampling needle to a position below the liquid level of the reaction tank specifically comprises:
and moving the sampling needle to be below the liquid level of the reaction tank until the end part of the sampling needle is positioned at the bottom of the reaction tank.
Preferably, the first liquid is latex, and the second liquid is a sample liquid.
Preferably, the moving the sampling needle below the liquid level of the reaction tank and controlling the pump to inject air into the liquid in the reaction tank by the sampling needle include:
moving the sampling needle to a position below the liquid level of the reaction tank;
and controlling the pump to start according to a preset frequency, so that the sampling needle injects air into the liquid in the reaction tank for multiple times.
A sample analyzer comprises a sampling needle, a pump, a pipeline for connecting the sampling needle and the pump, a moving device for driving the sampling needle to move and a reaction tank, wherein at least two liquids are uniformly mixed by adopting the sample uniformly mixing method according to any one of claims 1 to 9.
The sample blending method provided by the invention comprises the following steps: moving the sampling needle to a first position, and controlling the pump to enable the sampling needle to suck a first liquid from the first position; moving the sampling needle to the reaction tank, and controlling the pump to enable the sampling needle to inject first liquid into the reaction tank; moving the sampling needle to a second position, and controlling the pump to enable the sampling needle to suck a second liquid from the second position; moving the sampling needle to the reaction tank, and controlling the pump to enable the sampling needle to inject a second liquid into the reaction tank; moving the sampling needle to be above the liquid level of the reaction tank, and controlling the pump to enable the sampling needle to suck air; and moving the sampling needle to a position below the liquid level of the reaction tank, and controlling the pump to enable the sampling needle to inject air into the liquid in the reaction tank. According to the sample mixing method provided by the invention, the mixing place is in the reaction tank, the pollution degree of the detection part and the corresponding liquid pipe caused by the traditional sucking, spitting and mixing mode can be greatly reduced, the liquid is prevented from being diluted, the detection precision of the subsequent liquid is improved, meanwhile, the actions of injecting the liquid and sucking and spitting air are integrated and completed in the sampling needle, the preparation and mixing efficiency can be effectively improved, in addition, the sampling and mixing work can be completed by using the same pump and the same sampling needle, the requirement on the system is low, the equipment is favorably simplified, and the cost is reduced.
The sample analyzer comprises a sampling needle, a pump, a pipeline for connecting the sampling needle and the pump, a moving device for driving the sampling needle to move and a reaction tank, wherein the sampling needle is arranged in the reaction tank; the sample analyzer adopts the sample blending method, and the sample blending method can achieve the technical effects, so the sample analyzer can achieve the technical effects.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of one embodiment of a sample blending method provided by the present invention;
FIG. 2 is a flowchart of a first embodiment of a sample blending method according to the present invention;
FIG. 3 is a flowchart of a second embodiment of a sample blending method according to the present invention;
FIG. 4 is a flowchart of a third embodiment of a sample blending method provided by the present invention;
FIG. 5 is a schematic diagram of one embodiment of a sample analyzer according to the present invention;
wherein: 101-detection component, 102-reaction pool, 103-sampling needle, 104-syringe pump and 105-cleaning solution holding cavity.
Detailed Description
The core of the application is to provide a sample mixing method and a sample analyzer, which can reduce the structural requirements on a system and improve the preparation mixing efficiency of samples.
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Referring to fig. 1 to 5, fig. 1 is a flowchart illustrating a sample blending method according to an embodiment of the present invention; FIG. 2 is a flowchart of a first embodiment of a sample blending method according to the present invention; FIG. 3 is a flowchart of a second embodiment of a sample blending method according to the present invention; FIG. 4 is a flowchart of a third embodiment of a sample blending method provided by the present invention; fig. 5 is a schematic structural diagram of an embodiment of the sample analyzer provided in the present invention.
In this embodiment, the sample blending method is applied to a blending system, the blending system includes a sampling needle 103, a pump, a pipeline for connecting the sampling needle 103 and the pump, a moving device for driving the sampling needle 103 to move, and a reaction tank 102, and the pump can provide power for the sampling needle 103.
As shown in fig. 1, the sample blending method includes:
step S1: moving the sampling needle 103 to a first position, and controlling the pump to enable the sampling needle 103 to suck the first liquid from the first position;
step S2: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject the first liquid into the reaction tank 102;
step S3: moving the sampling needle 103 to a second position, and controlling the pump to enable the sampling needle 103 to suck a second liquid from the second position;
step S4: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject a second liquid into the reaction tank 102;
step S5: moving the sampling needle 103 to be above the liquid level of the reaction tank 102, and controlling the pump to enable the sampling needle 103 to suck air;
step S6: the sampling needle 103 is moved to a position below the liquid level in the reaction cell 102, and the pump is controlled so that the sampling needle 103 injects air into the liquid in the reaction cell 102.
Further, when it is necessary to mix three or more liquids, after step S4, the method further includes:
moving the sampling needle 103 to a third position, and controlling the pump to enable the sampling needle 103 to suck third liquid from the third position;
the sampling needle 103 is moved to the reaction cell 102, and the pump is controlled so that the sampling needle 103 injects the third liquid into the reaction cell 102.
On the basis of the above embodiments, the pump is the syringe pump 104, the material of the syringe pump 104 is convenient to obtain, and the injection rod of the syringe pump 104 is provided with the electric pump, so that the pushing control of the injection rod is convenient, and the precision is improved; a cleaning liquid storage chamber 105 is provided in the syringe pump 104.
Preferably, the syringe pump 104 can be vertically disposed with its opening facing upward, and during the process of moving the injection rod up and down to suck the air column or suck and discharge the liquid, the cleaning liquid will be located at the lower part of the cleaning liquid accommodating chamber 105 under the action of gravity, so as to avoid flowing into the pipeline and polluting the liquid.
Further, after step S2, the method further includes:
step S2-1: controlling the pump to make the cleaning liquid in the cleaning liquid accommodating cavity 105 flow out along the pipeline and the sampling needle 103;
step S2-2: the outer surface of the sampling needle 103 is air dried.
According to the steps, after the liquid is sucked every time, the inner wall and the outer wall of the sampling needle 103 can be cleaned, so that pollution to other liquids is avoided, and the detection precision is improved.
Similarly, after step S4, the method further includes:
step S4-1: controlling the pump to make the cleaning liquid in the cleaning liquid accommodating cavity 105 flow out along the pipeline and the sampling needle 103;
step S4-2: the outer surface of the sampling needle 103 is air dried.
Further, specifically, before step S1, the method further includes: controlling the pump to enable the sampling needle 103 to suck an air column; before step S3, the method further includes: the pump is controlled to cause the sampling needle 103 to draw a column of air. By sucking the column of air between the sampling needle 103 and the cleaning liquid containing chamber 105, the liquid of the sampling needle 103 can be prevented from contacting the cleaning liquid in the cleaning liquid containing chamber 105, avoiding contamination.
In addition to the above embodiments, the moving device includes a sampling needle 103 swinging member;
step S6 is followed by:
step S7: the sampling needle 103 is controlled to swing.
Specifically, the sampling needle 103 may swing left and right along a vertical plane, or swing along a ring, and specifically, a moving track of a swing component of the sampling needle 103 may be set as required.
In addition to the above embodiments, step S6 is executed to move the sampling needle 103 to a position below the liquid surface of the reaction cell 102, and then to control the pump to control the sampling needle 103 to oscillate while injecting air into the liquid in the reaction cell 102.
The above-mentioned steps can be performed simultaneously by controlling the oscillation of the sampling needle 103 while injecting air into the liquid in the reaction cell 102 by the sampling needle 103, i.e., simultaneously performing the steps S6 and S7, thereby simultaneously operating the syringe pump 104 and the oscillating member of the sampling needle 103.
Of course, in practical implementation, only step S6 may be executed, that is, only the sampling needle 103 is used to inject air into the liquid in the reaction cell 102; in addition, after the sampling needle 103 injects air into the liquid in the reaction cell 102 after the completion of the step S6, the sampling needle 103 may be controlled to swing, that is, after the completion of the step S6, the step S7 may be performed. The three modes can be all adopted, and the method can be specifically implemented according to the needs.
On the basis of the above embodiments, the steps S5 and S6 are repeatedly performed at least twice, that is, after the steps S5 and S6 are completed, the steps S5 and S6 are repeated. In the above steps, through the stirring process of sucking and spitting air for many times, the uniform mixing effect of the liquid in the reaction tank 102 can be improved, and the detection precision is improved.
In addition to the above embodiments, when the syringe pump 104 is used, the step S6 includes:
moving the sampling needle 103 to be below the liquid level of the reaction tank 102;
the pump is controlled to start according to the preset frequency, so that the sampling needle 103 injects air into the liquid in the reaction tank 102 for multiple times. Specifically, the syringe pump 104 can inject air into the reaction tank 102 below the liquid level multiple times by pushing the injection rod multiple times in succession, thereby improving the mixing efficiency.
In addition to the above embodiments, the movement of the sampling needle 103 to a position below the liquid level of the reaction cell 102 is specifically:
the sampling needle 103 is moved to below the liquid level of the reaction cell 102 until the end of the sampling needle 103 is located at the bottom of the reaction cell 102.
In each of the above embodiments, the first liquid is latex, and the second liquid is a sample liquid. Specifically, the second liquid may be a protein sample liquid. Of course, the sample mixing method can also be applied to mixing other liquids, and is not limited to the types of liquids given in the embodiment.
In a specific embodiment, the sample blending method includes:
a certain amount of latex is sucked and distributed by the sampling needle 103 and placed in the reaction tank 102 to serve as bottom liquid of the reaction tank 102, after the sampling needle 103 is cleaned by cleaning liquid in the cleaning liquid accommodating cavity 105 and the outer wall of the sampling needle 103 is air-dried, a certain amount of pretreated sample liquid is sucked, the sampling needle 103 is moved downwards, and the sample liquid is distributed into the reaction tank 102;
the sampling needle 103 is lifted above the reaction tank 102 and is completely in the air, the injector pump 104 is pulled down to suck a certain air column, the sampling needle 103 is pulled down to the bottom of the reaction tank 102, the injector pump 104 is continuously and intermittently pushed up to pump out the air in the sampling needle 103 and the corresponding pipeline through a liquid outlet of the sampling needle 103 at a preset frequency, the sampling needle 103 swings back and forth in a matched manner, the sample mixed liquid is stirred by utilizing the pumped continuous air column in a matched manner with needle swinging, and the latex and the antigen are fully mixed repeatedly to achieve the effect of uniformly mixing the sample.
Further, if the antibody is required to be added subsequently, the sample solution can be uniformly mixed according to the obtaining mode of the latex or the sample solution; and finally, the well-mixed sample liquid can be directly pumped to a measuring device for measurement, and a result is obtained.
Example one
As shown in fig. 2, the sample blending method includes:
step S1: moving the sampling needle 103 to a first position, and controlling the pump to enable the sampling needle 103 to suck the first liquid from the first position;
step S2: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject the first liquid into the reaction tank 102;
step S2-1: controlling the pump to make the cleaning liquid in the cleaning liquid accommodating cavity 105 flow out along the pipeline and the sampling needle 103;
step S2-2: air-drying the outer surface of the sampling needle 103;
step S3: moving the sampling needle 103 to a second position, and controlling the pump to enable the sampling needle 103 to suck a second liquid from the second position;
step S4: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject a second liquid into the reaction tank 102;
step S4-1: controlling the pump to make the cleaning liquid in the cleaning liquid accommodating cavity 105 flow out along the pipeline and the sampling needle 103;
step S4-2: air-drying the outer surface of the sampling needle 103;
step S5: moving the sampling needle 103 to be above the liquid level of the reaction tank 102, and controlling the pump to enable the sampling needle 103 to suck air;
step S6: the sampling needle 103 is moved to a position below the liquid level in the reaction cell 102, and the pump is controlled so that the sampling needle 103 injects air into the liquid in the reaction cell 102.
Example two
As shown in fig. 3, the sample blending method includes:
step S1: moving the sampling needle 103 to a first position, and controlling the pump to enable the sampling needle 103 to suck the first liquid from the first position;
step S2: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject the first liquid into the reaction tank 102;
step S3: moving the sampling needle 103 to a second position, and controlling the pump to enable the sampling needle 103 to suck a second liquid from the second position;
step S4: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject a second liquid into the reaction tank 102;
step S5: moving the sampling needle 103 to be above the liquid level of the reaction tank 102, and controlling the pump to enable the sampling needle 103 to suck air;
step S6: after the sampling needle 103 is moved to a position below the liquid level in the reaction cell 102, the pump is controlled to inject air into the liquid in the reaction cell 102 from the sampling needle 103, and the oscillation of the sampling needle 103 is also controlled.
EXAMPLE III
As shown in fig. 4, the sample blending method includes:
step S1: moving the sampling needle 103 to a first position, and controlling the pump to enable the sampling needle 103 to suck the first liquid from the first position;
step S2: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject the first liquid into the reaction tank 102;
step S3: moving the sampling needle 103 to a second position, and controlling the pump to enable the sampling needle 103 to suck a second liquid from the second position;
step S4: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject a second liquid into the reaction tank 102;
step S5: moving the sampling needle 103 to be above the liquid level of the reaction tank 102, and controlling the pump to enable the sampling needle 103 to suck air;
step S6: moving the sampling needle 103 to be below the liquid level of the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject air into the liquid in the reaction tank 102;
step S7: the sampling needle 103 is controlled to swing.
Example four
The sample mixing method comprises the following steps:
step S1: moving the sampling needle 103 to a first position, and controlling the pump to enable the sampling needle 103 to suck the first liquid from the first position;
step S2: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject the first liquid into the reaction tank 102;
step S2-1: controlling the pump to make the cleaning liquid in the cleaning liquid accommodating cavity 105 flow out along the pipeline and the sampling needle 103;
step S2-2: air-drying the outer surface of the sampling needle 103;
step S3: moving the sampling needle 103 to a second position, and controlling the pump to enable the sampling needle 103 to suck a second liquid from the second position;
step S4: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject a second liquid into the reaction tank 102;
step S4-1: controlling the pump to make the cleaning liquid in the cleaning liquid accommodating cavity 105 flow out along the pipeline and the sampling needle 103;
step S4-2: air-drying the outer surface of the sampling needle 103;
step S5: moving the sampling needle 103 to be above the liquid level of the reaction tank 102, and controlling the pump to enable the sampling needle 103 to suck air;
step S6: after the sampling needle 103 is moved to a position below the liquid level in the reaction cell 102, the pump is controlled to inject air into the liquid in the reaction cell 102 from the sampling needle 103, and the oscillation of the sampling needle 103 is also controlled.
EXAMPLE five
Step S1: moving the sampling needle 103 to a first position, and controlling the pump to enable the sampling needle 103 to suck the first liquid from the first position;
step S2: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject the first liquid into the reaction tank 102;
step S2-1: controlling the pump to make the cleaning liquid in the cleaning liquid accommodating cavity 105 flow out along the pipeline and the sampling needle 103;
step S2-2: air-drying the outer surface of the sampling needle 103;
step S3: moving the sampling needle 103 to a second position, and controlling the pump to enable the sampling needle 103 to suck a second liquid from the second position;
step S4: moving the sampling needle 103 to the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject a second liquid into the reaction tank 102;
step S4-1: controlling the pump to make the cleaning liquid in the cleaning liquid accommodating cavity 105 flow out along the pipeline and the sampling needle 103;
step S4-2: air-drying the outer surface of the sampling needle 103;
step S5: moving the sampling needle 103 to be above the liquid level of the reaction tank 102, and controlling the pump to enable the sampling needle 103 to suck air;
step S6: moving the sampling needle 103 to be below the liquid level of the reaction tank 102, and controlling the pump to enable the sampling needle 103 to inject air into the liquid in the reaction tank 102;
step S7: the sampling needle 103 is controlled to swing.
Besides the sample blending method, the present application also provides a sample analyzer using the sample blending method, where the sample analyzer includes a sampling needle 103, a pump, a pipeline for connecting the sampling needle 103 and the pump, a moving device for driving the sampling needle 103 to move, and a reaction cell 102.
Further, the sample analyzer further comprises a detection part 101, wherein the detection part 101 is connected with the reaction tank 102 through a liquid pipe, and after the mixed sample liquid in the reaction tank 102 is pumped to the detection part 101, the measurement of a sample liquid measurement item is realized by using an optical detection module in the detection part 101; the reaction cell 102 is a main place for realizing the uniform mixing of the sample preparation; the sampling needle 103 not only can realize the accurate absorption and distribution of blood samples and reagents, but also can fully mix the mixed liquid by the air column absorbed by the sampling needle 103 and the needle swinging action of the mixed liquid in a matching way; the syringe pump 104 provides power for mixing the sample fluid.
The sample mixing method provided by the embodiment greatly reduces the influence of the diluent on the sample mixed liquid on the premise of ensuring the mixing effect, ensures the accuracy of the sample liquid to be detected, reduces the pollution degree of the reaction tank 102 and the corresponding pipeline, and directly reduces the cleaning difficulty of the protein detection assembly.
For other structures of the sample analyzer, please refer to the prior art, and the description is omitted here.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The sample blending method provided by the present application is described in detail above. The principles and embodiments of the present application are explained herein using specific examples, which are provided only to help understand the method and the core idea of the present application. It should be noted that, for those skilled in the art, it is possible to make several improvements and modifications to the present application without departing from the principle of the present application, and such improvements and modifications also fall within the scope of the claims of the present application.
Claims (10)
1. The sample blending method is characterized by being applied to a blending system which comprises a sampling needle (103), a pump, a pipeline for connecting the sampling needle (103) and the pump, a moving device for driving the sampling needle (103) to move and a reaction pool (102); the method comprises the following steps:
moving the sampling needle (103) to a first position, controlling the pump to make the sampling needle (103) suck a first liquid from the first position;
moving the sampling needle (103) to the reaction pool (102), and controlling the pump to enable the sampling needle (103) to inject a first liquid into the reaction pool (102);
moving the sampling needle (103) to a second position, controlling the pump to enable the sampling needle (103) to suck a second liquid from the second position;
moving the sampling needle (103) to the reaction pool (102), and controlling the pump to enable the sampling needle (103) to inject a second liquid into the reaction pool (102);
moving the sampling needle (103) to be above the liquid level of the reaction tank (102), and controlling the pump to enable the sampling needle (103) to suck air;
moving the sampling needle (103) to be below the liquid level of the reaction pool (102), and controlling the pump to enable the sampling needle (103) to inject air into the liquid in the reaction pool (102).
2. The sample mixing method according to claim 1, wherein the pump is a syringe pump (104), and a cleaning solution containing chamber (105) is arranged in the syringe pump (104);
after the controlling the pump to enable the sampling needle (103) to inject the first liquid into the reaction cell (102), the method further comprises the following steps:
controlling the pump to enable the cleaning liquid in the cleaning liquid containing cavity (105) to flow out along the pipeline and the sampling needle (103);
air-drying the outer surface of the sampling needle (103).
3. The sample mixing method according to claim 2, wherein after controlling the pump to inject the second liquid into the reaction cell (102) through the sampling needle (103), the method further comprises:
controlling the pump to enable the cleaning liquid in the cleaning liquid containing cavity (105) to flow out along the pipeline and the sampling needle (103);
air-drying the outer surface of the sampling needle (103).
4. The sample mixing method according to claim 2, wherein before moving the sampling needle (103) to the first position and controlling the pump to suck the first liquid from the first position by the sampling needle (103), the method further comprises: controlling the pump to cause the sampling needle (103) to suck a column of air;
before the moving the sampling needle (103) to the second position and controlling the pump to make the sampling needle (103) suck the second liquid from the second position, the method further comprises: controlling the pump to cause the sampling needle (103) to draw a column of air.
5. The sample mixing method according to claim 1, wherein the moving means comprises a sampling needle (103) swinging member;
the moving of the sampling needle (103) to a position below the liquid level of the reaction pool (102) further comprises:
controlling the sampling needle (103) to swing.
6. The sample mixing method according to claim 5, wherein after the sampling needle (103) is moved to a position below the liquid level in the reaction cell (102), the pump is controlled to control the sampling needle (103) to oscillate while injecting air into the liquid in the reaction cell (102) from the sampling needle (103).
7. The sample mixing method according to any one of claims 1 to 6, wherein the moving of the sampling needle (103) to a position below the liquid level of the reaction cell (102) specifically comprises:
moving the sampling needle (103) to be below the liquid level of the reaction tank (102) until the end of the sampling needle (103) is positioned at the bottom of the reaction tank (102).
8. The method for uniformly mixing a sample according to any one of claims 1 to 6, wherein the first liquid is latex, and the second liquid is a sample liquid.
9. The sample mixing method according to any one of claims 2 to 4, wherein the moving the sampling needle (103) to a position below the liquid level of the reaction cell (102) and the controlling the pump to inject air into the liquid in the reaction cell (102) by the sampling needle (103) comprises:
moving the sampling needle (103) to a position below the liquid level of the reaction tank (102);
controlling the pump to start according to a preset frequency, and enabling the sampling needle (103) to inject air into the liquid in the reaction tank (102) for multiple times.
10. A sample analyzer, comprising a sampling needle (103), a pump, a pipeline for connecting the sampling needle (103) and the pump, a moving device for driving the sampling needle (103) to move, and a reaction cell (102), characterized in that at least two liquids are uniformly mixed by the sample uniformly mixing method according to any one of claims 1 to 9.
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