CN104535776A - Immune scatter turbidimetry based full-automatic detection device and method thereof - Google Patents
Immune scatter turbidimetry based full-automatic detection device and method thereof Download PDFInfo
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- CN104535776A CN104535776A CN201410736378.8A CN201410736378A CN104535776A CN 104535776 A CN104535776 A CN 104535776A CN 201410736378 A CN201410736378 A CN 201410736378A CN 104535776 A CN104535776 A CN 104535776A
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Abstract
The invention discloses an immune scatter turbidimetry based full-automatic detection device and a method thereof. By means of a sampling needle and two reagent needles, individual loading of a sample and double reagent needles can be realized, cross contamination between liquid reagents can be avoided, and the detection accuracy can be improved. The sample adding and cleaning between channels are independent to each other, thus greatly improving the efficiency of the detection device. The reaction cup can be used repeatedly, not only saves cost, but also realizes miniaturization and intelligentization of immune scatter turbidimetry full-automatic detection equipment, thus being better suitable for the detection needs of hospitals and small clinics. At the same time, a magnetic field transformation bottom spiral eddy current mixing and cleaning technology is adopted to achieve good stirring and cleaning effects of the reaction cup.
Description
Technical field
The present invention relates to field of medical devices, refer more particularly to a kind of full-automatic detection apparatus based on Immune scatter turbidimetry and method thereof.
Background technology
Immune scatter turbidimetry, refer to that the light of specific wavelength is propagated in the horizontal direction, when through solution, run into Ag-Ab immune response and composite particles in conjunction with formation time, light can reflect, and deflects, and forms scattered light, scattered beam now has a deflection angle compared to horizontal direction, this angle size is combined formed grain size with radiative wavelength and Ag-Ab, quantity is closely related.In test side, by detecting its scattered light intensity, the antigen concentration in solution to be measured can be extrapolated.
The current existing checkout equipment based on Immune scatter turbidimetry on the market, there is following defect: 1) reaction cup of most of checkout equipment is disposable, not reproducible use, each detection all needs again to change reaction cup on the one hand, make testing cost high, cause realizing the full-automatic of complete machine on the other hand; 2) even if the reaction cup of some checkout equipment is capable of washing reusable, but be all adopt existing comparatively ripe discrete reaction cup (being similar to the cuvette in Biochemical Analyzer), this structure make total part and machine volume huge, the miniaturization of equipment cannot be realized; 3) stirring apparatus of reaction cup is arranged on reaction cup side more, and rotates by the magnet being arranged on reaction cup side the rotation controlling reaction cup internal magnetization stirrer, but the reaction cup of this kind of alr mode is disposable, can not reuse; Even if adopt non-reusable reaction cup, but there is stirrer and in rotary course, very easily block the fluid path passage of reaction cup and affect the problem of stirring.4) load detection sample by single sampling needle and detect reagent, make the easy residual liquid of sampling needle, affect testing result, even if there is cleaning, but cleaning way is the mode that sampling needle absorption cleaning fluid is discharged again, also can there is the sordid problem of cleaning, and then cause reagent mix and affect testing result.
Therefore, prior art needs further to be improved.
Summary of the invention
Problem to be solved by this invention is, a kind of full-automatic detection apparatus based on Immune scatter turbidimetry and method thereof are provided, solve the technical matters that turbidimetry checkout equipment cannot realize fully-automated synthesis, device miniaturization, stirring and washing uneven and single sampling needle loading detection sample and detect reagent.
The present invention is by the following technical solutions:
A kind of full-automatic detection apparatus based on Immune scatter turbidimetry, comprise cabinet and be arranged on the two-dimentional machinery arm in cabinet, reaction cup and reagent position, described reaction cup is plugged in optical detection module, two-dimentional machinery arm controls driven by motor by control system and moves, also comprise separately for application of sample or simultaneously for the sampling needle of application of sample and cleaning, separately detect reagent or simultaneously for adding at least one reagent needles detecting reagent and cleaning for adding, and the fluid path support be slidably mounted on two-dimentional machinery arm, described sampling needle is arranged on described fluid path support, described reagent needles is arranged on above described fluid path support or reaction cup, fluid path water channel is communicated with bottom described reaction cup.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, when described sampling needle separately for application of sample and/or reagent needles separately for add detect reagent time, described device also comprises cleaning needle, and described cleaning needle is arranged on above reaction cup.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described reagent needles comprises the first reagent needles and the second reagent needles, and described first reagent needles is arranged on above reaction cup, and described second reagent needles is arranged on fluid path support.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described second reagent needles and cleaning needle are arranged side by side and the two is fixed by fixed support.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described support bracket fastened one end is hinged on described cabinet, and the other end is fastened on cabinet.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described reaction cup place is provided with stirring apparatus, and described stirring apparatus includes motor, be arranged on motor and glass magnetic part of internal magnetization change and be positioned over magnetic stirring of reaction cup inside of inducing reaction when rotated.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described magnetic part is arranged on below or the top of described reaction cup or arranges around reaction cup surrounding.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described magnetic part is two opposite polarity magnet of the opposite face be arranged side by side and is arranged on below or the top of described reaction cup; Or described magnetic part is ring-shaped magnet or arc-shaped magnet and arranges around reaction cup surrounding.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described stirrer is placed horizontally in bottom reaction cup.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described stirrer is that centre has protruding column construction.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described reaction cup includes many groups, and is all provided with cleaning needle and/or the second reagent needles above every group reaction cup.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described cleaning needle and/or the second reagent needles are broken line type structure.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described two-dimentional machinery arm comprises Horizontal machine mechanical arm and vertical mechanical arm, Horizontal machine mechanical arm is fixed in described cabinet, described vertical mechanical arm relative level mechanical arm is slidably installed, and described fluid path bracket slide is arranged on described vertical mechanical arm.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, the put area of detected sample is positioned at cabinet outside, and arranges starting switch on the cabinet wall at the put area place of detected sample, draws detected sample for starting sampling needle from put area.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described sampling needle and reagent needles are all connected with load pump, and wherein, the load pump connecting sampling needle is ram pump, and the load pump connecting the first reagent needles and the second reagent needles is fixed displacement pump.
The described full-automatic detection apparatus based on Immune scatter turbidimetry, wherein, described control system stirs for setting detected parameters and automatically controlling two-dimentional machinery arm movement velocity, sampling needle application of sample, reagent needles loading reagent, cleaning device cleaning and stirring apparatus according to the detected parameters of setting.
Based on a fully-automated synthesis method for Immune scatter turbidimetry, comprise step:
Control system controls two-dimentional machinery arm, sampling needle is moved to the put area of detected sample, draws detected sample, and moves to reaction cup place, added by detected sample in reaction cup;
Control system loads first by the first reagent needles and detects reagent in reaction cup, and starts stirring apparatus, stirring reaction cup;
After the first detection reagent and detected sample have reacted, control system has loaded second by the second reagent needles and has detected reagent in reaction cup, and starts stirring apparatus, stirring reaction cup;
After the second detection reagent and mixed liquor have reacted, measured the scattered light intensity of solution in reaction cup by Immune scatter turbidimetry, calculated the content of composition to be measured in detected sample, and waste liquid is discharged;
Control system starts cleaning device, is added by cleaning fluid in reaction cup, and start stirring apparatus, stirring reaction cup, discharges cleaning fluid after completing.
The described fully-automated synthesis method based on Immune scatter turbidimetry, wherein, the step of described startup stirring apparatus, stirring reaction cup specifically comprises: the electric motor starting of stirring apparatus, drive magnetic part rotates, reaction cup internal magnetic field is changed, and then drives magnetic stirring in reaction cup to rotate.
The described fully-automated synthesis method based on Immune scatter turbidimetry, wherein, after having added detected sample or detection reagent, control system controls corresponding load pump and cleaning fluid is added the first reagent needles or the second reagent needles or sampling needle, cleans respectively to the inside and outside wall of the first reagent needles or the second reagent needles or sampling needle.
The described fully-automated synthesis method based on Immune scatter turbidimetry, wherein, control system controls each sense channel respectively and detects separately.
Compared with prior art, the full-automatic detection apparatus based on Immune scatter turbidimetry of the present invention and detection method create following technical advantages: 1) reaction cup is reusable, achieve the fully-automated synthesis of Immune scatter turbidimetry; 2) adopt the bottom spiral vortex of magnetic field conversion to stir and evenly mix and cleaning technique, realization response cup preferably stirs and cleaning performance; 3) sampling needle and double reagent pin are set respectively, realize the independent loading of sample and two reagent needles, avoid the cross pollution of sample and reagent in loading procedure, make testing result more accurate; 4) application of sample between each passage and cleaning are separate, substantially increase the efficiency of whole pick-up unit.
Accompanying drawing explanation
Fig. 1 is the structural representation of the full-automatic detection apparatus based on Immune scatter turbidimetry provided by the invention.
Fig. 2 is the structural representation based on two-dimentional machinery arm in the full-automatic detection apparatus of Immune scatter turbidimetry provided by the invention.
Fig. 3 is the structural representation based on two-dimentional machinery arm and stirring apparatus in the full-automatic detection apparatus of Immune scatter turbidimetry provided by the invention.
Fig. 4 is the structural representation based on stirring apparatus in the full-automatic detection apparatus of Immune scatter turbidimetry provided by the invention.
Fig. 5 is the cut-open view based on stirring apparatus in the full-automatic detection apparatus of Immune scatter turbidimetry provided by the invention.
Fig. 6 is the process flow diagram of the fully-automated synthesis method based on Immune scatter turbidimetry provided by the invention.
Fig. 7 is the process flow diagram of the preferred embodiment of the fully-automated synthesis method based on Immune scatter turbidimetry provided by the invention
Embodiment
In order to make technical matters to be solved by this invention, technical scheme and beneficial effect clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
As shown in Figures 1 to 5, full-automatic detection apparatus based on Immune scatter turbidimetry provided by the invention, comprise cabinet 10, chamber door 11 and be arranged on two-dimentional machinery arm 12 in cabinet 10, reaction cup 15, reagent position 16, cleaning device 14, stirring apparatus 20 and optical detection module 19, reaction cup 15 is plugged in optical detection module 19, and two-dimentional machinery arm 12 controls driven by motor by control system and moves.Wherein, also comprise separately for application of sample or simultaneously for application of sample and cleaning sampling needle 131, detect reagent or simultaneously for adding at least one reagent needles (132 and/or 142) detecting reagent and cleaning and the fluid path support 13 be slidably mounted on two-dimentional machinery arm 12 for adding separately, described sampling needle 131 is arranged on described fluid path support 13, and described reagent needles (132 and/or 142) is arranged on above described fluid path support 131 or reaction cup 15.Sampling needle can realize adding detection reagent and cleaning, then without the need to arranging special cleaning needle if can realize application of sample and cleaning or reagent needles simultaneously simultaneously; And if sampling needle separately for application of sample or reagent needles separately for adding detection reagent, then need to arrange special cleaning needle.
Preferably, reagent needles of the present invention is set to two, comprises the first reagent needles 142 and the second reagent needles 132, first reagent needles 142 is preferably mounted at above reaction cup 15, for loading the first detection reagent; Second reagent needles is preferably mounted on fluid path support 13, in the present embodiment, preferred sampling needle, reagent needles and cleaning needle are arranged separately, independent passage walked separately by often kind of liquid reagent, avoid the cross pollution between liquid reagent, interchannel can walk abreast operations such as carrying out sample, test analysis, cleaning, does not affect each other, makes testing result more accurate.
Again see Fig. 2, two-dimentional machinery arm 12 of the present invention comprises Horizontal machine mechanical arm 121 and vertical mechanical arm 122, Horizontal machine mechanical arm 121 is fixed in described cabinet 10, described vertical mechanical arm 122 relative level mechanical arm 121 is slidably installed, and described fluid path support 13 is slidably mounted on described vertical mechanical arm 122, the motion of such two-dimentional machinery arm 12 can drive the motion of fluid path support 13, and then drive fluid path support 13 up-sampling pin 131 move to detected sample place, realize detected sample and detect the loading of reagent.And in concrete loading procedure, when sampling needle 131 moves to corresponding position, sampling needle 131 on fluid path support 13 can stretch downwards and carry out suction operation in detected sample, sampling needle 131 upwards can be packed up after absorption completes, and move to above reaction cup 15, sampling needle 131 can be flexible downwards, joined by detected sample in reaction cup 15.And the loading detecting reagent is undertaken by load pump, first reagent needles 142 and the second reagent needles 132 are connected to load pump (not shown), load pump connects the reagent position 16 holding the first detection reagent and the second detection reagent respectively, when loading, directly from reagent position 16, draw corresponding detection reagent by load pump, and by corresponding reagent needles, detection reagent is directly added in reaction cup.As can be seen from said process, detected sample of the present invention and detection reagent load respectively by the sampling needle arranged separately and corresponding reagent pin, separate, effectively can avoid the cross pollution between sample or reagent.
The put area of detected sample of the present invention is positioned at cabinet 10 outside, is convenient to practical operation like this.The cabinet wall at the put area place of detected sample arranges starting switch 17, drawing detected sample for starting sampling needle from put area, making application of sample process more intelligent.
Further, stirring apparatus 20 of the present invention can be arranged on below reaction cup or top, or arrange around reaction cup surrounding, preferably, stirring apparatus 20 is arranged on below reaction cup 15, as shown in Figure 4 and Figure 5, include motor 21, magnetic stirring 23 being arranged on the magnetic part 22 on motor 21 and being positioned over bottom reaction cup 15, heat treatment above magnetic part 22 can cause when being rotated by driven by motor, by the conversion in magnetic field, drive magnetic stirring 23 in reaction cup 15 followed by horizontally rotate together, magnetic stirring 23 rotates at the bottom of reaction cup 15 glasss, the liquid in reaction cup 15 is made to form eddy current, thus play and stir and evenly mix, and the effect of cleaning.The magnetic part 22 of the present embodiment is preferably two magnet be arranged side by side, and two magnet is contrary to opposite polarity, thus ensure the N pole of lines of magnetic induction from a magnet, to the S pole of another magnet, and then by the attraction between magnet, magnetic stirring in reaction cup 15 is adsorbed on the bottom of reaction cup 15, and certain magnetic part also can be other magnetic structural component, its can cause when being rotated by driven by motor above heat treatment.Owing to being communicated with fluid path water channel 20 bottom reaction cup 15, therefore for avoiding stirrer to block fluid path water channel in whipping process, stirrer 23 is preferably placed horizontally in bottom reaction cup 15, and its length is less than the diameter of reaction cup.
Above-mentioned stirring apparatus 20 is arranged on for preferred version below reaction cup, only for explaining the technical scheme making reaction cup internal magnetic field change and then drive stirrer to rotate by stirring apparatus, and and be not used in restriction.Stirring apparatus is arranged on above reaction cup and also can, now, magnetic part is identical with the scheme arranged below reaction cup, be opposite polarity two magnet of opposite face, can be induced reaction a glass internal magnetic field change when rotated, and then magnetic stirring in drive reaction cup followed by and horizontally rotates together, thus play the effect stirred and evenly mixed.
If stirring apparatus is around being arranged on reaction cup surrounding, then magnetic part is ring-shaped magnet or arc-shaped magnet, when magnetic part is ring-shaped magnet, magnet is the non-homogeneous structure that magnetizes of different parts, and magnetic part is arc-shaped magnet, can be orthodrome shape magnet, now magnet along the size at two ends, rounded face direction or thickness all not identical, to make two ends have different magnetic field intensity, glass internal magnetic field that induces reaction when reaction cup rotates sends change, and then drive stirrer to rotate, play the effect of stirring reaction cup.Above-mentioned several scheme is preferred version, and other are changed by change reaction cup internal magnetic field and drive the scheme of stirrer rotation.
Preferably, stirrer is that centre has two protruding column constructions, in stirrer rotary course, due to two projections, can form two whirlpools in liquid again, make mixing effect better.
Stirring apparatus 20 and optical detection module 19 Integral design, arrange separately in the position of optical detection module 19 placing response cup 15 and stirring apparatus region is installed, with conserve space, and stirring apparatus is arranged on below reaction cup and also can realizes better stirring operation, improve stirring efficiency.
Further, in order to realize intelligent cleaning in fully-automated synthesis process, as shown in Figure 3, cleaning device 14 of the present invention to be arranged on above reaction cup 15 and the cleaning needle 141 of cleaning device 14 just to reaction cup 15, cleaning device 14 and response location are fixed, the complete cleaning fluid that all can directly automatically load of each detection is in reaction cup 15, in cleaning process, stirring apparatus stirs reaction cup 15, cleaning fluid is fully cleaned reaction cup 15, after having cleaned, discharges reaction cup 15 by fluid path water channel.Optical detection module 19 of the present invention and reaction cup 15 include many groups, for organizing sample detection more.Above every group reaction cup 15, cleaning needle 141 is all installed, is preferably every group reaction cup 15 and two cleaning needles 141 are set, with maximal efficiency, clean in place is carried out to every group reaction cup 15.Particularly, in the present embodiment, cleaning device 14 is arranged on after vertical mechanical arm 122, and cleaning needle 141 and the first reagent needles 142 are fixedly mounted on a fixed support 143 side by side, cleaning needle 141 and the first reagent needles 142 are broken line type structure, comprise two vertical sections and a horizontal segment, wherein a vertical section is arranged on fixed support, another vertical section is positioned at above reaction cup 15, this kind of vibrational power flow can be avoided affecting two-dimentional machinery arm and fluid path support motion, and then can not have an impact to sampling needle and the second reagent needles application of sample.Preferably, one end of fixed support 143 is hinged on cabinet, the other end is fastened on cabinet, make fixed support 143 can folding, facilitate the replacing of cleaning needle and the first reagent needles 142, during use, fixed support is opened, change cleaning needle or the first reagent needles 142, and then be fastened on cabinet, operate very simple.
Further, sampling needle 131, first reagent needles 142 of the present invention and the second reagent needles 132 are all connected with load pump, and the load pump connecting sampling needle 131 is ram pump, and the load pump connecting the first reagent needles 142 and the second reagent needles 132 is fixed displacement pump.
Control system of the present invention for set detected parameters and automatically control two-dimentional machinery arm 12 movement velocity according to the detected parameters of setting, sampling needle 131 application of sample, the second reagent needles 132 load reagent, cleaning device 12 cleans and stirring apparatus stirs.The fully-automated synthesis of detected sample can be realized by this control system.Preferably, this control system includes human-computer interaction interface 18, and by human-computer interaction interface 18, operator can carry out the setting of various detected parameters more easily.
Based on above-mentioned full-automatic detection apparatus, present invention also offers a kind of fully-automated synthesis method based on Immune scatter turbidimetry, as shown in Figure 6, comprise step:
Step S100, control system control two-dimentional machinery arm, sampling needle are moved to the put area of detected sample, draw detected sample, and move to reaction cup place, added by detected sample in reaction cup;
Step S200, control system load first by the first reagent needles and detect reagent in reaction cup, and start stirring apparatus, stirring reaction cup;
Step S300, after the first detection reagent and detected sample have reacted, control system has loaded the second detection reagent by the second reagent needles in reaction cup, and starts stirring apparatus, stirring reaction cup;
Step S400, after the second detection reagent and mixed liquor have reacted, measured the scattered light intensity of solution in reaction cup by Immune scatter turbidimetry, calculated the content of composition to be measured in detected sample, and waste liquid is discharged;
Step S500, control system start cleaning device, are added by cleaning fluid in reaction cup, and start stirring apparatus, stirring reaction cup, discharges cleaning fluid after completing.
Further, the step of described startup stirring apparatus, stirring reaction cup specifically comprises: the electric motor starting of stirring apparatus, drives magnetic part to rotate, reaction cup internal magnetic field is changed, and then drives magnetic stirring in reaction cup to rotate.By this detection method, the present invention can realize the fully-automated synthesis of specific protein, and can the abundant stirring of realization response cup and cleaning in testing process, can reach extraordinary Detection results and very high detection efficiency.
In order to avoid reagent needles and sampling needle residual liquid and affect subsequent detection, the present invention can to sampling needle, the inside and outside of the first reagent needles and the second reagent needles is cleaned, particularly, after having added detected sample or detection reagent, control system startup connects sampling needle respectively, the corresponding load pump of the first reagent needles or the second reagent needles, draw cleaning fluid and cleaning fluid directly added in sampling needle or the first reagent needles or the second reagent needles, respectively the inside and outside wall of sampling needle or the first reagent needles or the second reagent needles is cleaned with backwashing manner, timely maintenance sampling needle or the first reagent needles or the second reagent needles inside and outside clean, detect reagent or sample carryover after effectively avoiding each detection and affect subsequent detection, improve accuracy of detection.
Below in conjunction with specific embodiment, above-mentioned testing process is described in detail and is illustrated.
First, sample is added.Sampling needle draws sample in the sample position in detected sample region, sample is drawn into inside sampling needle, then the motion of two-dimentional machinery arm is controlled, above reaction cup sampling needle being moved on to one of them passage (such as passage 1), then, the first reagent needles above this passage is controlled by control system, add in the reaction cup of this passage and detect reagent R1, and then control sampling needle and move down into below reaction cup liquid level, in reaction cup, quantitatively sample is added by the accurate control of control system, after adding sample, whole system starts stirring automatically, the sample mixing in reaction cup.
Secondly, detection reagent R2 is added.After adding detection reagent R1 and sample, through the enough reaction time, the second reagent needles that control system controls above two-dimentional machinery arm automatically moves to correct position, add automatically and accurately and detect reagent R2, after having added, control system starts stirring apparatus automatically, and all liq in reaction cup is stirred and evenly mixed.
Moreover system detects sample content automatically.After having added detection reagent R1, sample, detection reagent R2, whole device has detected automatically.Meanwhile, sampling needle returns to initial load position, and return in the process of initial load position at sampling needle, whole device controls automatically, realizes cleaning the inside and outside wall of sampling needle, for sampling is prepared next time.
Finally, cleaning reaction cup.After that Air conduct measurement in above-mentioned steps completes, system can control the cleaning needle above this passage automatically, automatically in the reaction cup of this passage, inject cleaning fluid, by the changes of magnetic field below reaction cup, drive magnetic stirring in reaction cup to rotate, strengthen the cleaning dynamics to reaction cup inside, finally by negative pressure, the waste liquid in reaction cup is discharged again, through the fluid injection too much taken turns, clean, discharge opeing, completes the cleaning to this passage automatically.Meanwhile, after sampling needle returns to initial load position, step one can be repeated to step 3, carry out the detection to next sample, and pick-up unit automatically cleans and can carry out independently of each other with the detection of next sample simultaneously.
In above-mentioned testing process, the application of sample between each passage and cleaning are separate, and such as passage 1 is at application of sample, can clean other passages such as passage 2 grade.Thus, the efficiency of whole pick-up unit is substantially increased.Simultaneously, for each passage, sampling needle, two reagent needles (reagent 1 and reagent 2) and cleaning needle are all provided separately, the loading pipeline of all liq be all separately, separate, thus the cross pollution avoided well between reagent, substantially increase accuracy and reliability that this device detects automatically.
Be described further for system control process below, as shown in Figure 7, comprise step:
Step S1: press switch activated detection;
Step S2: quantitatively pipette samples;
Step S3: whether judgement sample has been drawn, performs step S4 if completed, otherwise continues to perform step S2;
Step S4: sampling needle rises and resets;
Step S5: judge whether sampling needle has resetted, performs step S6 if completed, otherwise performs step S7;
Step S6: sampling needle stops cleaning moving to reaction channel;
Step S7: system controls cleaning sampling needle outer wall automatically; Cleaning sampling needle outer wall mainly cleans sample residual on outer wall, avoid sampling needle move with adding procedure in scatter, the more important thing is that sample that when avoiding adding in reaction cup, outer wall is residual drops in reaction cup and sample size is increased, affect testing result.
Step S8: tell tip portion sample; To eliminate motor hysterisis error, ensure application of sample accuracy;
Step S9: the first reagent needles adds reagent R1;
Step S10: control ram pump and accurately add sample;
Step S11: control to start and stir and uniform stirring sample;
Step S12: sampling needle rises and resets;
Step S13: judge whether sampling needle has resetted, performs step S14 if completed, otherwise performs step S15;
Step S14: system controls cleaning sampling needle inwall automatically;
Step S15: system controls cleaning sampling needle outer wall wall automatically;
Step S16: sample and reagent R1 react;
Step S17: whether detection reaction completes, performs step S18 if completed, otherwise performs step S16;
Step S18: the second reagent needles moves to reaction channel;
Step S19: automatic powder adding reagent adding R2;
Step S20: judge whether that interpolation completes, performs step S21 if added, otherwise continues to perform step S19;
Step S21: start and stir;
Step S22: current channel detects automatically;
Step S23: judge whether detection completes, perform step S25 if completed, otherwise continue to perform step S22;
Step S24: after execution of step S22, judges whether to continue the next sample of test by other reaction channels; If it is return step S1, otherwise perform step S23;
Step S25: the automatic cleaning reaction cup of Systematical control cleaning needle;
Step S26: control to start and stir enhancing cleaning performance;
Step S27: judge whether that cleaning completes, performs step S28 if completed, otherwise continues to perform step S26;
Step S28: control cleaning needle interpolation reactant liquor and be immersed in reaction cup;
Step S29: complete.
In sum, the full-automatic detection apparatus based on Immune scatter turbidimetry provided by the invention and method thereof, by by sampling needle and two reagent needles, realize the independent loading of sample and double reagent pin, avoid the cross pollution between liquid reagent, promotes accuracy in detection; Reaction cup is reusable, not only cost-saving, and achieves the miniaturized intelligent of fully-automated synthesis equipment of Immune scatter turbidimetry, has the detection demand being better suitable for hospital, mini clinic; Meanwhile, adopt the bottom spiral vortex of magnetic field conversion to stir and evenly mix and cleaning technique, realization response cup preferably stirs and cleaning performance.
Should be understood that, application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (20)
1. the full-automatic detection apparatus based on Immune scatter turbidimetry, comprise cabinet and be arranged on the two-dimentional machinery arm in cabinet, reaction cup, reagent position and cleaning device, described reaction cup is plugged in optical detection module, two-dimentional machinery arm controls driven by motor by control system and moves, it is characterized in that, also comprise separately for application of sample or simultaneously for the sampling needle of application of sample and cleaning, separately detect reagent or simultaneously for adding at least one reagent needles detecting reagent and cleaning for adding, and the fluid path support be slidably mounted on two-dimentional machinery arm, described sampling needle is arranged on described fluid path support, described reagent needles is arranged on above described fluid path support or reaction cup, fluid path water channel is communicated with bottom described reaction cup.
2. the full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 1, it is characterized in that, when described sampling needle separately for application of sample and/or reagent needles separately for add detect reagent time, described device also comprises cleaning needle, and described cleaning needle is arranged on above reaction cup.
3. the full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 2, it is characterized in that, described reagent needles comprises the first reagent needles and the second reagent needles, and described first reagent needles is arranged on above reaction cup, and described second reagent needles is arranged on fluid path support.
4. the full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 3, is characterized in that, described second reagent needles and cleaning needle are arranged side by side and the two is fixed by fixed support.
5. the full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 4, is characterized in that, described support bracket fastened one end is hinged on described cabinet, and the other end is fastened on cabinet.
6. the full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 3, it is characterized in that, described reaction cup place is provided with stirring apparatus, and described stirring apparatus includes motor, be arranged on motor and glass magnetic part of internal magnetization change and be positioned over magnetic stirring of reaction cup inside of inducing reaction when rotated.
7. the full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 6, is characterized in that, described magnetic part is arranged on below or the top of described reaction cup or arranges around reaction cup surrounding.
8. the full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 6, is characterized in that, described magnetic part is two opposite polarity magnet of the opposite face be arranged side by side and is arranged on below or the top of described reaction cup; Or described magnetic part is ring-shaped magnet or arc-shaped magnet and arranges around reaction cup surrounding.
9. the full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 6, is characterized in that, described stirrer is placed horizontally in bottom reaction cup.
10. the full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 6, is characterized in that, described stirrer is that centre has protruding column construction.
11. full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 4, it is characterized in that, described reaction cup includes many groups, and is all provided with cleaning needle and/or the second reagent needles above every group reaction cup.
12. full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 4, is characterized in that, described cleaning needle and/or the second reagent needles are broken line type structure.
13. full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 1, it is characterized in that, described two-dimentional machinery arm comprises Horizontal machine mechanical arm and vertical mechanical arm, Horizontal machine mechanical arm is fixed in described cabinet, described vertical mechanical arm relative level mechanical arm is slidably installed, and described fluid path bracket slide is arranged on described vertical mechanical arm.
14. full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 1, it is characterized in that, the put area of detected sample is positioned at cabinet outside, and starting switch is set on the cabinet wall at the put area place of detected sample, draw detected sample for starting sampling needle from put area.
15. full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 1, it is characterized in that, described sampling needle, the first reagent needles and the second reagent needles are all connected with load pump, wherein, the load pump connecting sampling needle is ram pump, and the load pump connecting the first reagent needles and the second reagent needles is fixed displacement pump.
16. full-automatic detection apparatus based on Immune scatter turbidimetry according to claim 1, it is characterized in that, described control system stirs for setting detected parameters and automatically controlling two-dimentional machinery arm movement velocity, sampling needle application of sample, reagent needles loading reagent, cleaning device cleaning and stirring apparatus according to the detected parameters of setting.
17. 1 kinds, based on the fully-automated synthesis method of Immune scatter turbidimetry, is characterized in that, comprise step:
Control system controls two-dimentional machinery arm, sampling needle is moved to the put area of detected sample, draws detected sample, and moves to reaction cup place, added by detected sample in reaction cup;
Control system loads first by the first reagent needles and detects reagent in reaction cup, and starts stirring apparatus, stirring reaction cup;
After the first detection reagent and detected sample have reacted, control system has loaded second by the second reagent needles and has detected reagent in reaction cup, and starts stirring apparatus, stirring reaction cup;
After the second detection reagent and mixed liquor have reacted, measured the scattered light intensity of solution in reaction cup by Immune scatter turbidimetry, calculated the content of composition to be measured in detected sample, and waste liquid is discharged;
Control system starts cleaning device, is added by cleaning fluid in reaction cup, and start stirring apparatus, stirring reaction cup, discharges cleaning fluid after completing.
The 18. fully-automated synthesis methods based on Immune scatter turbidimetry according to claim 17, it is characterized in that, the step of described startup stirring apparatus, stirring reaction cup specifically comprises: the electric motor starting of stirring apparatus, drive magnetic part rotates, reaction cup internal magnetic field is changed, and then drives magnetic stirring in reaction cup to rotate.
The 19. fully-automated synthesis methods based on Immune scatter turbidimetry according to claim 17, it is characterized in that, after having added detected sample or detection reagent, control system controls corresponding load pump and cleaning fluid is added sampling needle or the first reagent needles or the second reagent needles, cleans respectively to the inside and outside wall of sampling needle or the first reagent needles or the second reagent needles.
The 20. fully-automated synthesis methods based on Immune scatter turbidimetry according to claim 17, it is characterized in that, control system controls each sense channel respectively and detects separately.
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