CN110244070A - A kind of specific protein analyzer and its test method - Google Patents
A kind of specific protein analyzer and its test method Download PDFInfo
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- CN110244070A CN110244070A CN201910444735.6A CN201910444735A CN110244070A CN 110244070 A CN110244070 A CN 110244070A CN 201910444735 A CN201910444735 A CN 201910444735A CN 110244070 A CN110244070 A CN 110244070A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
Abstract
The invention discloses a kind of specific protein analyzer and its test methods, specific protein analyzer includes casing and rack, rack includes the front end panel with operation panel interval before casing, with the rear bearing sheet at computer case back plate interval, before, the two-dimentional arm restraining board perpendicular to bottom plate is equipped between rear bearing sheet, the X-axis component of automatic two dimension arm is mounted on two-dimentional arm restraining board, the side of two-dimentional arm restraining board is equipped with and the spaced circuit partition of computer case back plate, Control card is mounted on circuit partition, between two-dimentional arm restraining board and casing, the fluid path partition perpendicular to bottom plate is equipped between circuit partition and front end panel, it is equipped with and bottom plate horizontal supporting plate disposed in parallel between front end panel and rear bearing sheet, horizontal supporting plate is located at the lower position that two-dimentional arm restraining board is equipped with the side of automatic two-dimentional arm, reagent storage module and reaction mould group are solid It is scheduled on horizontal supporting plate, reagent storage module includes at least two reagent storage positions, and reaction mould group is more than two even numbers.
Description
Technical field
The present invention relates to a kind of specific protein analyzer and its test methods, belong to protein analyzer technical field.
Background technique
With being constantly progressive for social medical level, specific protein analyzer is had begun in each large and medium-sized doctor in recent years
Institute is widely used, clinically to provide essential information foundation to the diagnosis of disease, treatment and prevention and health status.
But the full-automatic specific protein analyzer of current main-stream is mostly used for the measurement of inflammation CRP index, and with facing
The development of bed technique, a CRP index cannot fully meet demand, can provide in conjunction with SAA index for clinical detection and more may be used
The judgment basis leaned on.Simultaneously, it is contemplated that inflammation detection usually combines the part index number of cellanalyzer, therefore, support whole blood
The direct examination with computer of sample can simplify operation for clinical trial person, save the time.At present mostly using two different apparatus measures
The two indexs of CRP and SAA inhale sample twice and not only affect the accuracy of measurement, but also increase the dosage of sample, additional increasing
The workload of tester is added, in addition, also taking up the instrument placing space of hospital.Existing CRP+SAA all-in-one machine is to adopt
Analysis test is carried out with biochemical mode or immunochromatography mode, the CRP+SAA all-in-one machine that biochemical mode is tested has reaction
The structures such as disk, reagent disc, structure is not compact enough, and volume is larger, and cannot handle whole blood；The all-in-one machine of immunochromatography mode because
The reason of methodology, test result are influenced bigger by factors such as raw material, productions, and precision is poor.Therefore, the prior art
All-in-one machine haves the shortcomings that volume is larger or accuracy of measurement is lower.A volume of Vehicles Collected from Market urgent need is small as far as possible, test is accurate
Spend high CRP+SAA all-in-one machine.
Summary of the invention
The purpose of the present invention is to provide the specific protein analyzers that one kind can test CRP and SAA simultaneously, it is intended to solve
The problem that current CRP+SAA all-in-one machine volume is larger, test accuracy is not high.The present invention also provides a kind of specific proteins point simultaneously
The test method of analyzer.
The present invention adopts the following technical scheme: a kind of specific protein analyzer, including casing and the machine being arranged in casing
Frame, casing include two side plates in left and right, the preceding operation panel being arranged between left and right side plates and backboard and top plate, preceding operation panel
It is equipped with display screen, output unit and sample introduction position, rack includes bottom plate, and automatic two-dimentional arm, reaction mould are equipped in rack
Group, reagent punch block, reagent storage module, liquid storage module and Control card, automatic two dimension arm includes rack-mounted X-axis group
Part and the Y-axis component moved back and forth in X-axis component, Y-axis component lower end are equipped with swab, are equipped with sampling moving up and down in swab
Needle, being located at before casing on operation panel has the space being pierced by for sampling needle above sample introduction position, the rack include with before casing
The spaced front end panel of operation panel and the spaced rear bearing sheet of computer case back plate, between front end panel and rear bearing sheet
Equipped with the two-dimentional arm restraining board perpendicular to bottom plate, the side of two-dimentional arm restraining board be equipped with the spaced circuit of computer case back plate every
Plate, Control card are mounted on circuit partition, in the region that two-dimentional arm restraining board and casing surround, circuit partition and front end panel it
Between be equipped with fluid path partition perpendicular to bottom plate, the liquid storage module is mounted on fluid path partition, circuit partition, front end panel and casing
In the region surrounded, the automatic two-dimentional arm is mounted on the other side of two-dimentional arm restraining board, the X-axis component installation of automatic two dimension arm
On two-dimentional arm restraining board, the X-axis component level of automatic two dimension arm is arranged and front and back extends, and offers confession on the front end panel
The two-dimentional arm perforation that automatic two dimension arm X-axis front end and Y-axis component are pierced by, is equipped with and bottom plate between front end panel and rear bearing sheet
Horizontal supporting plate disposed in parallel, horizontal supporting plate are located at the lower orientation that two-dimentional arm restraining board is equipped with the side of automatic two-dimentional arm
It sets, the reagent storage module and reaction mould group are fixed on horizontal supporting plate, and reagent storage module includes at least two reagents
Storage position is equipped with reagent storage bottle in each reagent storage position, and reaction mould group is more than two even numbers, each to react mould group
Front and back is arranged in same level, and each reaction pedestal for reacting mould group left and right extends, and the reagent punch block is connected by hinge
It connects on the position being located between two-dimentional arm and reaction mould group on two-dimentional arm restraining board, the reagent punch block is equipped with and reacts mould
The equal reagent needle assemblies of group quantity, each reagent needle assemblies are correspondingly arranged at the top of each reaction mould group reaction cup, each
Reagent needle assemblies include the reaction solution reagent needle connecting with liquid storage module and cleaning solution reagent needle, reaction solution reagent needle and cleaning
Liquid reagent needle passes through reaction liquid pipeline respectively and cleaning solution and reaction solution is added in cleaning liquid pipeline into reaction cup respectively, each to react
Cup rim of a cup and each reagent storage bottle bottleneck are located on the same straight line of approximately the same plane, the Y-axis of the straight line and automatic two-dimentional arm
In one plane, and the plane is perpendicular to horizontal plane for the track formed when component moves horizontally.
There is gap, one end of each reaction pedestal for reacting mould group is solid between the bottom edge and bottom plate of the two dimension arm restraining board
It is scheduled on horizontal supporting plate, the other end is passed through from the gap between two-dimentional arm restraining board and bottom plate.
The reaction mould group and reagent storage module are each attached on the bottom surface of horizontal supporting plate, on the horizontal supporting plate
The position of corresponding each reaction cup rim of a cup and reagent bottle bottleneck is equipped with opening.
The circuit partition and fluid path partition are respectively positioned on two-dimentional arm restraining board leftward position, and the horizontal supporting plate is located at two
Arm restraining board right positions are tieed up, rear bearing sheet is located at the right positions of two-dimentional arm restraining board rear end.
Stirring motor in the reaction mould group is located at reaction cup right positions on reaction pedestal, reacts the optics in mould group
Channel is located at reaction cup leftward position on reaction pedestal, and the output shaft of stirring motor is horizontally disposed, and axis is connected on output shaft
Horizontally disposed magnet turntable, magnet turntable is equipped with close to reaction cup lower part, in reaction cup to be rotated under magnet driven by rotary disc
Without magnetic stirring bar.
The reagent storage module includes that refrigeration pedestal, the refrigeration outer cover being fixed on refrigeration pedestal and setting are being freezed
Refrigeration inner core and semiconductor chilling plate in outer cover freeze and are equipped with heat-preservation cotton, the reagent storage between outer cover and refrigeration inner core
Position setting is in refrigeration inner core, and refrigeration inner core is close to the chill surface of cooling piece, and the heating surface of semiconductor chilling plate, which is sticked, to be located at
Cooling fin outside refrigeration outer cover, cooling fin are equipped with radiator fan far from the side of refrigeration outer cover.
The quantity of the reaction mould group is four, and the reagent needle assemblies are four groups, and every group reagent needle assemblies are arranged every
The top of a reaction mould group reaction cup.
The bottom plate, front end panel, rear bearing sheet, two-dimentional arm restraining board, circuit partition, fluid path partition and horizontal supporting plate
It is panel beating part.
The analysis method analyzed using specific protein analyzer comprising following steps: (1) select test item for
Single CRP or the bis- projects of list SAA or CRP+SAA；(2) sample to be detected is fitted into test tube, is grasped before test tube is then placed in casing
Make at the sample introduction position on plate；(3) by adjusting two-dimentional arm, Y-axis component is made to drive sampling needle mobile, sampling needle is grasped before casing
Make to be pierced by sample introduction position on plate, quantitative suction sample is carried out to sample in test tube；It (4) will be in liquid storage module by reaction solution reagent needle
Reaction solution be added to it is corresponding reaction mould group reaction cup in；If the bis- projects of CRP+SAA pass through the first group reagent needle assemblies
Reaction solution reagent needle by reaction solution add first react mould group reaction cup in, pass through the reaction of two group reagent needle assemblies
Liquid reagent needle adds reaction solution in the reaction cup for reacting mould group at second；(5) by adjusting two-dimentional arm, sampling needle is inserted
Enter into the reaction mould group reaction cup for having added reaction solution, then the sample that sampling needle is drawn is added in the reaction cup, then
Sampling needle is cleaned by swab；If the bis- projects of CRP+SAA, sampling needle carries out double sampling, the sample that will be drawn twice
Originally be respectively added to first reaction mould group reaction cup neutralize second reaction mould group reaction cup in or sampling needle into
Row is primary to inhale sample, and the reaction cup that the sample of absorption is successively added to first reaction mould group is neutralized the anti-of second reaction mould group
It answers in cup, sampling needle is cleaned by swab after sample-adding；(6) starting stirring motor is to the reaction solution in group reaction cup
It is mixed with sample；(7) two-dimentional arm is adjusted, sampling needle is moved at reagent storage module, sampling needle quantitatively draws reagent
Reagent in storing bottle；(8) sampling needle is moved on to added in the reaction cup of reaction solution and sample, sampling needle is inserted into the reaction cup
It is middle that quantitative reagent is added to it；If the bis- projects of CRP+SAA, sampling needle first quantitatively draws the examination in first reagent storage bottle
The reagent is added in the reaction cup of first reaction mould group by agent, is cleaned after sampling needle and is quantitatively drawn using sampling needle again
The reagent is added in the reaction cup of second reaction mould group by the reagent in another reagent storage bottle, each reagent addition
After clean sampling needle；(9) reaction solution, sample and the reagent in reaction cup are mixed by stirring motor；(10) by anti-
Mould group is answered to be reacted sample, measured；(11) pass through Control card transmission measurement signal, and signal is converted, is calculated most
Terminate fruit；(12) testing result is shown by the display screen on casing, final result is exported by the output unit on casing；
(13) by reaction cup react after discharging of waste liquid to casing outside waste liquid barrel in, will be in liquid storage module by cleaning solution reagent needle
Cleaning solution be added in reaction cup, reaction cup is cleaned；If the bis- projects of CRP+SAA, in reaction cup after discharging of waste liquid,
It is in the reaction cup that cleaning solution addition is reacted mould group at first by the cleaning solution reagent needle of the first group reagent needle assemblies and right
It is cleaned, and is added cleaning solution in the reaction cup for reacting mould group at second by the cleaning solution reagent needle of two group reagent needle assemblies
And it is cleaned.
The beneficial effects of the present invention are: in protein analyzer of the invention, by rack front end panel, rear bearing sheet,
Two-dimentional arm restraining board, circuit partition, fluid path baffle arrangement make two-dimentional arm, Control card, fluid path group at the structure of similar II font
Part is arranged in different regions, sets two-dimentional arm, reagent punch block, reaction mould group successively from top to bottom by horizontal supporting plate
It sets, such layout type has the advantage that (1) each component compact layout, casing internal space in the rack in casing
Utilize maximization, it is ensured that instrument placing space also reaches minimum；(2) Control card and liquid are made by circuit partition and fluid path partition
Road component is in different spaces, to put fluid path and Control card are completely isolated, it is ensured that when fluid path breaks down not
Circuit can be caused to damage, avoid causing short circuit；(3) there are two reagent needles in a reagent needle assemblies of the invention, primary to inhale
Sample meets the test of two projects of CRP+SAA simultaneously, and reaches extremely low limit to sample consumption.
Preferably, there is gap between the bottom edge and bottom plate of two-dimentional arm restraining board, passed through from gap convenient for reaction mould group.
Preferably, horizontal supporting plate is located at two-dimentional arm restraining board right positions, corresponding that sample introduction position is made to be located at the casing right side
Side is to be measured, meets human engineering.
Preferably, using reaction module design, market master is lower than to optical utilization rate height, good water-proof effect, failure rate
Type is flowed, stirring rod can be adhered to certain not noticeable places to avoid stirring rod in this way and cause to user without being magnetic
Puzzlement, the design of side stirring can also solve the stirring rod in whipping process and fly out the exception of reaction cup.
Preferably, the quantity for reacting mould group is four, and reagent needle assemblies are four groups.
Detailed description of the invention
Fig. 1 is the complete machine external view of the specific protein analyzer of an embodiment of the present invention；
Fig. 2 is the interior layout figure of specific protein analyzer in Fig. 1；
Fig. 3 is the internal structure view one of specific protein analyzer in Fig. 1；
Fig. 4 is the internal structure view two of specific protein analyzer in Fig. 1 (reagent component turns over)；
Fig. 5 is the top view of Fig. 3；
Fig. 6 is the automatic two-dimentional arm configuration schematic diagram in Fig. 3；
Fig. 7 is the reaction modular structure schematic diagram in Fig. 3；
Fig. 8 is the reaction mould group cross-sectional view one of Fig. 3；
Fig. 9 is the reaction mould group cross-sectional view two of Fig. 3；
Figure 10 is the reagent storage modular structure schematic diagram in Fig. 3；
Figure 11 is reagent storage module cross-sectional view in Fig. 3；
Figure 12 is the schematic diagram of automatic two-dimentional arm, reaction mould group, reagent punch block in Fig. 3；
Figure 13 is the test method flow chart of specific protein analyzer of the present invention.
In figure, 100- casing, 101- display screen, 102- switch, 103- output unit, 104- sample introduction position, 200- rack,
201- two dimension arm restraining board, 202- fluid path partition, 203- circuit partition, 204- front end panel, 205- rear bearing sheet, 206- are horizontal
Support plate, 207- bottom plate, 300- reagent punch block, the first reagent needle of 301-, 302-, 303- the second reagent needle, 304-, 305-,
400- two dimension arm, 401-X shaft assembly, 402-Y shaft assembly, 403- sampling needle, 404- swab, 405- two dimension arm support, 500- are anti-
Mould group is answered, 501- reacts pedestal, and 502- Heating Cup, 503- reaction cup, 504- laser, 505- opto-electronic receiver plate, 506- is without magnetic
Stirring rod, 507- stirring motor, 508- magnet turntable, 509- leakage fluid dram, 800- reagent storage module, 801- refrigeration pedestal,
802- refrigeration outer cover, 803- refrigeration inner core, 804- heat-preservation cotton, 805- semiconductor chilling plate, 806- cooling fin, 807- fan,
808- temperature sensor, 809- reagent storage box.
Specific embodiment
The present invention is described in detail with specific embodiment below in conjunction with the accompanying drawings.
The structure of the specific protein analyzer of an embodiment of the present invention is as shown in Figures 1 to 12, the present embodiment it is specific
Protein analyzer includes casing 100 and the rack 200 that is arranged in casing 100.
The structure of casing 100 is as shown in Figure 1, casing 100 includes two side plates in left and right, is arranged between left and right side plates
Preceding operation panel and backboard and top plate, preceding operation panel are equipped with display screen 101, output unit 103 and sample introduction position 104, sample introduction
The namely sample to be tested placement region of position 104 is equipped on the casing outer wall at sample to be tested placement region and starts switch, opens
Dynamic switch is manual key switch, and manual key switch 102 is used for the manipulation of manual measurement, and sample to be tested placement region is located at machine
At the position of the lower right side of shell operation panel, operator's right hand holds the test tube equipped with sample, penetrates in sample needle, and right ring finger is light
It touches manual key switch 102 and opens test.Such design meets human engineering, also makes sample-adding more intelligent.Display screen and defeated
Unit controls outputting and inputting for this specific protein analyzer respectively out, and display screen 101 is touching display screen, can be used for setting
Detection parameters, and the automatic two-dimentional movement of arm, the suction of sample needle and reagent needle, liquid are automatically controlled according to the detection parameters of setting
Whether the work of road device component etc..The output unit is used for output test result, and output unit can use printer, may be used also
To be data output interface or printer interface.
Rack 200 includes bottom plate 207, and automatic two-dimentional arm 400, reaction mould group 500, reagent punch block are equipped in rack 200
300, reagent storage module 800, liquid storage module and Control card, plane figure of all parts in rack 200 such as Fig. 2 institute
Show.The liquid storage module is connect by fluid path component with corresponding component, and fluid path component includes plunger pump, diaphragm pump, solenoid valve etc..
The structure of automatic two dimension arm 400 is as shown in fig. 6, automatic two dimension arm 400 includes rack-mounted X-axis component
401 and in the Y-axis component 402 that X-axis component 401 moves back and forth, 402 lower end of Y-axis component is equipped with swab 404, is equipped in swab 404
Sampling needle 403 moving up and down, being located at before casing on operation panel has the sky being pierced by for sampling needle above sample introduction position 104
Between, the structure of the automatic two-dimentional arm 400 of the present embodiment is same as the prior art, and X-axis component 401 and Y-axis component 402 are using leading
Rail+synchronous belt linear motion design method improves the operation stability of automatic two-dimentional arm 400.Movement one in horizontal direction
Seven stop positions are shared, includes a sample bits, four reaction cup positions and two reagent positions, is controlled respectively using seven optocouplers
This seven stop positions, seven optocouplers use independent connection, avoid the influence of step out of stepping motor, improve the accurate of movement
Degree.On Y-axis component 402, sampling needle 403 can move up and down under the drive of Y-axis synchronous belt, and swab 404 passes through two
Sebific duct is connected with fluid path, is cleaned by the inside and outside wall that sampling needle 403 may be implemented in the control of pump valve, sampling needle 403 is not only used to
Sample sample-adding is inhaled, but also the reagent in reagent bottle can be drawn, and be added in reaction cup 503.Different liquids are inhaled in sampling needle 403
During need to sampling needle 403 carry out inside and outside wall cleaning, with guarantee be not in cross contamination, specific operating process
Are as follows: sample-sample-adding-cleaning-suction reagent-reagent adding-cleaning is inhaled, is designed by this unique timing control, to sampling needle 403
Inside and outside wall cleaning effect is splendid, and clinical automatic two-dimentional arm 400 passes through two-dimentional arm support 405 and is mounted on the rack.
As shown in Fig. 3 to 5, the rack 200 include with the spaced front end panel 204 of operation panel before casing 100, with
Computer case back plate spaced rear bearing sheet 205 is equipped between front end panel 204 and rear bearing sheet 205 perpendicular to bottom plate 207
Two-dimentional arm restraining board 201, the side of two-dimentional arm restraining board 201 be equipped with the spaced circuit partition 203 of 100 backboard of casing,
Control card is mounted on circuit partition 203, in the region that two-dimentional arm restraining board 201 and casing 100 surround, 203 He of circuit partition
The fluid path partition 202 perpendicular to bottom plate is equipped between front end panel 204, the liquid storage module is mounted on fluid path partition 202, circuit
In the region that partition 203, front end panel 204 and casing 100 surround, the automatic two-dimentional arm 400 is mounted on two-dimentional arm restraining board
The X-axis component 401 of 201 other side, automatic two dimension arm 400 is mounted on two-dimentional arm restraining board 201, the X-axis of automatic two dimension arm
Component 401 is horizontally disposed and front and back extends, and is offered on the front end panel 204 for automatic two-dimentional 401 front end of arm X-axis and Y
The two-dimentional arm perforation that shaft assembly 402 is pierced by, is equipped with disposed in parallel with bottom plate 207 between front end panel 204 and rear bearing sheet 205
Horizontal supporting plate 206, horizontal supporting plate 206 are located at the lower section that two-dimentional arm restraining board 201 is equipped with the side of automatic two-dimentional arm 400
Position, the reagent storage module 800 and reaction mould group 500 are fixed on horizontal supporting plate 206, and reagent storage module 800 is wrapped
At least two reagent storage positions 809 are included, reagent storage bottle 809 is equipped in each reagent storage position, reaction mould group 500 is two
Above even number is respectively arranged in same level before and after reaction mould group 500, and each reaction pedestal 501 for reacting mould group 500 is equal
Left and right extends.The bottom plate 207, front end panel 204, rear bearing sheet 205, two-dimentional arm restraining board 201, circuit partition 203, fluid path
Partition 202 and horizontal supporting plate 206 are panel beating part.
As shown in figure 12, the reagent punch block 300 is connected by a hinge is located at automatic two dimension on two-dimentional arm restraining board 201
On position between arm 400 and reaction mould group 500, the reagent punch block 300 is equipped with the examination equal with reaction 500 quantity of mould group
Agent needle assemblies 301, each reagent needle assemblies 301 are correspondingly arranged at the top of each reaction 500 reaction cup 503 of mould group, Mei Geshi
Agent needle assemblies 301 include the reaction solution reagent needle 302 and cleaning solution reagent needle 303 connecting with liquid storage module, reaction solution reagent
Cleaning is added by reaction liquid pipeline and cleaning liquid pipeline respectively in needle 302 and cleaning solution reagent needle 303 into reaction cup 503 respectively
Liquid and reaction solution, each 503 rim of a cup of reaction cup and each reagent storage bottle bottleneck are located on the same straight line of approximately the same plane, this is straight
In one plane, and the plane is perpendicular to water for the track that the Y-axis component 402 of line and automatic two-dimentional arm is formed when moving horizontally
Plane.After a reaction cup 503 completes a test, the waste liquid in the reaction cup 503 can pass through the influidic pipe where it
Road is discharged into the waste liquid barrel of liquid storage module, and the cleaning solution reagent needle 303 of 503 top of reaction cup can be injected into reaction cup 503
Appropriate cleaning solution cleans reaction cup, and then waste liquid is discharged into the waste liquid barrel of liquid storage module, and 2 ~ 3 times repeatedly to complete to reaction
The cleaning of cup 503.After cleaning, injected by the 503 top reaction solution reagent needle 302 of reaction cup into the reaction cup 503
A certain amount of reaction solution waits the beginning tested next time.A brief overview only is done to cup cleaning herein, in test stream below
Cheng Zhongzai is described in detail.
The quantity that mould group 500 is reacted in the present embodiment is four, and the reagent needle assemblies 301 are also four groups, every group reagent
The top of each reaction 500 reaction cup of mould group is arranged in needle assemblies 301.Reaction solution reagent needle in each reagent needle assemblies 301
Pass through pipeline respectively with cleaning solution reagent needle to connect with liquid storage module, is respectively used to add reaction solution and cleaning into reaction cup 503
Liquid, reaction solution reagent needle and cleaning solution reagent needle fluid path are non-interference independently of each other, and reagent punch block 301 is fixed on machine by hinge
On frame 304, reagent punch block 301 can drive two reagent needles to turn over together, in order to replace reaction cup 503.Four reaction moulds
The test independently of one another of group 500, the cross contamination between avoiding, and when some channel is broken down, instrument still may be used
With proper testing.
There is gap, each reactive group for reacting mould group 500 between the bottom edge and bottom plate 207 of the two dimension arm restraining board 201
One end of seat 501 is fixed on horizontal supporting plate 206, the other end is from the gap between two-dimentional arm restraining board 201 and bottom plate 207
It passes through.
The reaction mould group 500 and reagent storage module 800 are each attached on the bottom surface of horizontal supporting plate 206, the water
The position that each reaction cup rim of a cup and reagent bottle bottleneck are corresponded in flat support plate 206 is equipped with opening.
The circuit partition 203 and fluid path partition 202 are respectively positioned on two-dimentional 201 leftward position of arm restraining board, the horizontal branch
Fagging 206 is located at 201 right positions of two-dimentional arm restraining board, and rear bearing sheet 205 is located at the right lateral position of 201 rear end of two-dimentional arm restraining board
It sets.
The structure of mould group is reacted as shown in Fig. 7 to body 9, is reacted the stirring motor 507 in mould group 500 and is located at reaction pedestal
503 right positions of reaction cup on 501 react the optical channel in mould group 500 and are located at reaction cup left lateral position on reaction pedestal 501
It sets, the place opposite with stirring motor is equipped with transparent reaction cup 503 on the reaction pedestal, is equipped with heating below reaction cup 503
Cup 502, be equipped in Heating Cup 502 electronic components such as heating rod, temperature sensor for the liquid in reaction cup 503 into
Row incubation with heat, stirring motor 507 are located at the inclined lower position in right side of Heating Cup 502, and the output shaft level of stirring motor 507 is set
It sets, the magnet turntable 508 of axis horizontal setting is connected on output shaft, magnet turntable 508 is close to reaction cup lower part, reaction cup
Be equipped in 503 rotated under the drive of magnet turntable 508 without magnetic stirring bar 506.The magnetic field of magnet turntable 508 drives stirring rod
506 make rotating motion together, achieve the purpose that mix the liquid in reaction cup 502.React pedestal rear and front end difference
Placing response light source and photoelectric receiving transducer, the reaction light source are on reacting track route and horizontal direction in pedestal
Certain angle, the reaction pedestal lower section sebific duct connect a filter, pass through sebific duct and liquid storage module phase below filter
Even.Conventional type is different from the prior art, stirring motor is placed in the mentality of designing reacted below mould group, the present embodiment will stir
Motor 507 is placed on the side of reaction mould group 500, and requires stirring rod 506 without being magnetic, in this way can be to avoid stirring rod 506
It is adhered to certain not noticeable places to cause to perplex to user, the design of side stirring can also solve to stir in whipping process
Stick 506 is mixed to fly out the exception of reaction cup 503.The rear side for reacting pedestal 501 is equipped with laser 504 as reaction light source, reactive group
The front side of seat 501 is equipped with opto-electronic receiver plate 505 and is used as photoelectric receiving transducer, and laser 504 and opto-electronic receiver plate 505, which utilize, to be dissipated
Penetrate detection of the turbidimetry realization to sample.After a test is completed, the waste liquid in reaction cup 503 can pass through leakage fluid dram 509
Place, is discharged by fluid path in the waste liquid barrel of liquid storage module.
Reagent storage module has refrigerating function, and as shown in Figure 10 to Figure 11, reagent storage module 800 includes refrigeration pedestal
801, the refrigeration outer cover 802 and the refrigeration inner core 803 being arranged in refrigeration outer cover 802 and half being fixed on refrigeration pedestal 801
Conductor cooling piece 805, refrigeration pedestal 801 are connected with refrigeration outer cover 802 with screw, are freezed between outer cover 802 and refrigeration inner core 803
Equipped with heat-preservation cotton 804, the setting of reagent storage position 809 is in refrigeration inner core 803, there is reagent storage position 809 in the present embodiment
Two, there is cold storage function, be respectively used to storage CRP and SAA emulsion reagent.The inner core 803 that freezes is close to semiconductor chilling plate 805
Chill surface, the heating surface of semiconductor chilling plate 805 be sticked have be located at refrigeration outer cover 801 outside cooling fin 806, cooling fin
806 sides far from refrigeration outer cover are equipped with radiator fan 807.Cooling fin 806 is connected on refrigeration outer cover 802 with screw, refrigeration
The bottom of inner core 803 is also equipped with temperature sensor 808.When reagent storage module 800 enters working condition, semiconductor refrigerating
The chill surface of piece 805 freezes to refrigeration inner core 803, and radiator fan 807 cooperates cooling fin 806 to semiconductor chilling plate 805
It radiates, to guarantee its normal work.And cooperate temperature control panel card (positioned at instrument rear by temperature sensor 808
In Control card region, do not shown in figure) to guarantee that the temperature on refrigeration pedestal 801 reaches assigned temperature.Two reagent storages
Position 809 can store two kinds of emulsion reagents of CRP and SAA simultaneously, and primary sample of inhaling can carry out two kinds of CRP+SAA tests.
The specific protein analyzer of the present embodiment can operate instrument by touching display screen 101, can be automatic
It completes to inhale the sequence of operations such as sample, test, cleaning, it is convenient and efficient；The rack of specific protein of the present invention analysis is by by liquid storage mould
The layout that block and Control card are kept apart, influence when avoiding fluid path failure to circuit, and this highly integrated cloth
Office's design makes to walk pipe more neat and easy to repair with cabling；Specific protein analyzer four independent reaction mould groups of the present invention can
It is tested with complete independently, even if some reaction mould group breaks down, nor affects on instrument normal use, this modular design
It is also convenient for maintenance and production；There are two reagent storage positions for the reagent storage module of specific protein analyzer of the present invention, can be simultaneously
Two kinds of emulsion reagents of CRP and SAA are stored, primary sample of inhaling can carry out two kinds of CRP+SAA tests.
Beneficial effects of the present invention are as follows: (1) compact layout, and space utilization maximizes, it is ensured that instrument placing space reaches
It is minimum；(2) it puts liquid storage module and Control card are completely isolated, it is ensured that circuit will not be caused when fluid path breaks down
Damage；(3) it once inhales sample and meets the test of two projects of CRP+SAA simultaneously, and extremely low limit is reached to sample consumption；(4)
The reaction module design of innovation is lower than market mainstream model to optical utilization rate height, good water-proof effect, failure rate.
A kind of specific embodiment for the analysis method that specific protein analyzer of the present invention is analyzed, the analysis of the present embodiment
The process of method is as shown in figure 13 comprising following steps: (1) selecting test item is that list CRP or list SAA or CRP+SAA are bis-
Project；(2) sample to be detected is fitted into test tube, then test tube is placed at the sample introduction position before casing on operation panel；(3) lead to
The two-dimentional arm of adjustment is crossed, makes Y-axis component drive sampling needle mobile, sampling needle is pierced by sample introduction position on operation panel before casing, right
Sample carries out quantitative suction sample in test tube；(4) reaction solution in liquid storage module is added to by reaction solution reagent needle corresponding anti-
In the reaction cup for answering mould group；If the bis- projects of CRP+SAA, by the reaction solution reagent needles of the first group reagent needle assemblies by reaction solution
It adds in the reaction cup for reacting mould group at first, is added reaction solution by the reaction solution reagent needle of two group reagent needle assemblies
In the reaction cup of second reaction mould group；(5) by adjusting two-dimentional arm, sampling needle is inserted into the reaction for having added reaction solution
In mould group reaction cup, then the sample that sampling needle is drawn is added in the reaction cup, then sampling needle carried out by swab clear
It washes；If the bis- projects of CRP+SAA, sampling needle carries out double sampling, and the sample drawn twice is respectively added to first reaction
The reaction cup of mould group neutralizes in the reaction cup of second reaction mould group or sampling needle carries out primary suction sample, by the sample of absorption
This reaction cup for being successively added to first reaction mould group neutralizes in the reaction cup of second reaction mould group, passes through after sample-adding
Swab cleans sampling needle；(6) starting stirring motor in group reaction cup reaction solution and sample mix；(7) it adjusts
Whole two dimension arm, sampling needle is moved at reagent storage module, sampling needle quantitatively draws the reagent in reagent storage bottle；(8) will
Sampling needle moves on to, and quantitative examination is added to it added in the reaction cup of reaction solution and sample, sampling needle is inserted into the reaction cup
Agent；If the bis- projects of CRP+SAA, sampling needle first quantitatively draws the reagent in first reagent storage bottle, which is added to
In the reaction cup of first reaction mould group, cleans after sampling needle and quantitatively drawn in another reagent storage bottle using sampling needle again
Reagent, which is added in the reaction cup of second reaction mould group, cleans sampling needle after the addition of each reagent；(9) lead to
Stirring motor is crossed to mix reaction solution, sample and the reagent in reaction cup；(10) sample is carried out by reaction mould group anti-
It answers, measure；(11) pass through Control card transmission measurement signal, and signal is converted, calculates final result；(12) pass through casing
On display screen show testing result, final result is exported by output unit on casing；(13) after being reacted in reaction cup
Discharging of waste liquid to casing outside waste liquid barrel in, the cleaning solution in liquid storage module is added to by reaction cup by cleaning solution reagent needle
In, reaction cup is cleaned；If the bis- projects of CRP+SAA, in reaction cup after discharging of waste liquid, pass through the first group reagent needle assemblies
Cleaning solution reagent needle by cleaning solution add first react mould group reaction cup in and it is cleaned, pass through two group reagent needles
Cleaning solution is added in the reaction cup for reacting mould group at second and is cleaned to it by the cleaning solution reagent needle of component.
Above embodiments description is only place related to mainly improvement of the invention, and the present invention is not referred to or do not retouched in detail
The part stated is the same as those in the prior art, such as the specific knot of the principle of optic test, the composition of Control card, liquid storage module
Structure, circuit and principle of fluid path control etc., therefore the present invention is not described in detail.
Described above is only presently preferred embodiments of the present invention, and above-mentioned specific embodiment is not limitation of the present invention.
In technical idea scope of the invention, various modifications and modification can occur, all those skilled in the art according to
The done retouching of upper description, modifications or substitutions, belong to the range that the present invention is protected.
Claims (9)
1. a kind of specific protein analyzer, including casing and the rack being arranged in casing, casing includes two side plates in left and right, sets
Set preceding operation panel between left and right side plates and backboard and top plate, preceding operation panel be equipped with display screen, output unit and into
Sample position, rack include bottom plate, and automatic two-dimentional arm, reaction mould group, reagent punch block, reagent storage module, storage are equipped in rack
Liquid module and Control card, automatic two dimension arm include rack-mounted X-axis component and the Y-axis that moves back and forth in X-axis component
Component, Y-axis component lower end are equipped with swab, sampling needle moving up and down are equipped in swab, is located at sample introduction before casing on operation panel
There is the space being pierced by for sampling needle, it is characterised in that: the rack includes being arranged with operation panel interval before casing above position
Front end panel, with the spaced rear bearing sheet of computer case back plate, between front end panel and rear bearing sheet be equipped with perpendicular to bottom plate
Two-dimentional arm restraining board, the side of two-dimentional arm restraining board is equipped with and the spaced circuit partition of computer case back plate, Control card peace
Be equipped in the region that circuit partition, two-dimentional arm restraining board and casing surround, between circuit partition and front end panel perpendicular to
The fluid path partition of bottom plate, the region that the liquid storage module is mounted on fluid path partition, circuit partition, front end panel and casing surround
Interior, the automatic two-dimentional arm is mounted on the other side of two-dimentional arm restraining board, and it is solid that the X-axis component of automatic two dimension arm is mounted on two-dimentional arm
On fixed board, the X-axis component level of automatic two dimension arm is arranged and front and back extends, and is offered on the front end panel for automatic two-dimentional arm
The two-dimentional arm perforation that X-axis front end and Y-axis component are pierced by, is equipped with disposed in parallel with bottom plate between front end panel and rear bearing sheet
Horizontal supporting plate, horizontal supporting plate are located at the lower position that two-dimentional arm restraining board is equipped with the side of automatic two-dimentional arm, the examination
Agent storage module and reaction mould group are fixed on horizontal supporting plate, and reagent storage module includes at least two reagent storage positions, often
Reagent storage bottle is equipped in a reagent storage position, reaction mould group is more than two even numbers, each arrangement before and after reacting mould group
In same level, each reaction pedestal for reacting mould group left and right extends, and the reagent punch block is connected by a hinge in two dimension
It is located on the position between two-dimentional arm and reaction mould group on arm restraining board, the reagent punch block is equipped with and reacts mould group quantity phase
Deng reagent needle assemblies, each reagent needle assemblies be correspondingly arranged at it is each reaction mould group reaction cup top, each reagent needle group
Part includes the reaction solution reagent needle connecting with liquid storage module and cleaning solution reagent needle, reaction solution reagent needle and cleaning solution reagent needle
Cleaning solution and reaction solution are added into reaction cup by reaction liquid pipeline and cleaning liquid pipeline respectively respectively, each reaction cup rim of a cup and
Each reagent storage bottle bottleneck is located on the same straight line of approximately the same plane, and the Y-axis component of the straight line and automatic two-dimentional arm does water
Put down the track formed when movement in one plane, and the plane is perpendicular to horizontal plane.
2. specific protein analyzer according to claim 1, it is characterised in that: the bottom edge and bottom of the two dimension arm restraining board
There is gap, one end of each reaction pedestal for reacting mould group is fixed on horizontal supporting plate, the other end is solid from two-dimentional arm between plate
It is passed through in gap between fixed board and bottom plate.
3. specific protein analyzer according to claim 1, it is characterised in that: the reaction mould group and reagent storage module
It is each attached on the bottom surface of horizontal supporting plate, the position of each reaction cup rim of a cup and reagent bottle bottleneck is corresponded on the horizontal supporting plate
It is equipped with opening.
4. specific protein analyzer according to claim 1, it is characterised in that: the circuit partition and the equal position of fluid path partition
In two-dimentional arm restraining board leftward position, the horizontal supporting plate is located at two-dimentional arm restraining board right positions, and rear bearing sheet is located at two
Tie up the right positions of arm restraining board rear end.
5. specific protein analyzer according to claim 4, it is characterised in that: the stirring motor position in the reaction mould group
In reaction cup right positions on reaction pedestal, reacts the optical channel in mould group and is located at reaction cup leftward position on reaction pedestal,
The output shaft of stirring motor is horizontally disposed, the magnet turntable of axis horizontal setting is connected on output shaft, magnet turntable is close to anti-
Answer a glass lower part, be equipped in reaction cup rotated under magnet driven by rotary disc without magnetic stirring bar.
6. specific protein analyzer according to claim 1, it is characterised in that: the reagent storage module includes refrigeration base
Seat, the refrigeration outer cover being fixed on refrigeration pedestal and the refrigeration inner core and semiconductor chilling plate being arranged in refrigeration outer cover, system
Heat-preservation cotton is equipped between cooling outer cover and refrigeration inner core, in refrigeration inner core, refrigeration inner core is close to system for the reagent storage position setting
Cold chill surface, the heating surface of semiconductor chilling plate, which is sticked, the cooling fin being located at outside refrigeration outer cover, and cooling fin is far from system
The side of cooling outer cover is equipped with radiator fan.
7. specific protein analyzer according to claim 1, it is characterised in that: the quantity of the reaction mould group is four,
The reagent needle assemblies are four groups, and the top of each reaction mould group reaction cup is arranged in every group reagent needle assemblies.
8. specific protein analyzer according to claim 1, it is characterised in that: the bottom plate, front end panel, rear support
Plate, two-dimentional arm restraining board, circuit partition, fluid path partition and horizontal supporting plate are panel beating part.
9. the analysis method analyzed using the specific protein analyzer of claim 1, which is characterized in that it includes following step
It is rapid: (1) to select test item for list CRP or the bis- projects of list SAA or CRP+SAA；(2) sample to be detected is fitted into test tube, so
Test tube is placed at the sample introduction position before casing on operation panel afterwards；(3) by adjusting two-dimentional arm, Y-axis component is made to drive sampling needle
Mobile, sampling needle is pierced by sample introduction position on operation panel before casing, carries out quantitative suction sample to sample in test tube；(4) by anti-
Liquid reagent needle is answered to be added to the reaction solution in liquid storage module in the reaction cup of corresponding reaction mould group；If the bis- items of CRP+SAA
Mesh is added reaction solution in the reaction cup for reacting mould group at first by the reaction solution reagent needle of the first group reagent needle assemblies,
Reaction solution is added in the reaction cup for reacting mould group at second by the reaction solution reagent needle of two group reagent needle assemblies； (5)
By adjusting two-dimentional arm, sampling needle is inserted into the reaction mould group reaction cup for having added reaction solution, then sampling needle is drawn
Sample is added in the reaction cup, is then cleaned by swab to sampling needle；If the bis- projects of CRP+SAA, sampling needle into
The reaction cup that the sample drawn twice is respectively added to first reaction mould group is neutralized second reaction mould group by row double sampling
Reaction cup in or sampling needle carry out it is primary inhale sample, the sample of absorption is successively added to the anti-of first reaction mould group
It answers cup to neutralize in the reaction cup of second reaction mould group, sampling needle is cleaned by swab after sample-adding；(6) starting is stirred
Mix motor in group reaction cup reaction solution and sample mix；(7) two-dimentional arm is adjusted, sampling needle is moved to reagent and is deposited
At amplification module, sampling needle quantitatively draws the reagent in reagent storage bottle；(8) sampling needle is moved on to added with reaction solution and sample
In reaction cup, sampling needle is inserted into the reaction cup, and quantitative reagent is added to it；If the bis- projects of CRP+SAA, sampling needle is first fixed
Amount draws the reagent in first reagent storage bottle, which is added in the reaction cup of first reaction mould group, cleaning is adopted
The reagent in another reagent storage bottle is quantitatively drawn using sampling needle again after sample needle, which is added to second reaction
In the reaction cup of mould group, sampling needle is cleaned after each reagent addition；(9) by stirring motor to reaction solution, the sample in reaction cup
This and reagent are mixed；(10) sample reacted by reacting mould group, measured；(11) pass through Control card transmission measurement
Signal, and by signal conversion, calculate final result；(12) testing result is shown by the display screen on casing, passes through casing
On output unit export final result；(13) by the waste liquid barrel outside the discharging of waste liquid to casing after being reacted in reaction cup, lead to
Cleaning solution in liquid storage module is added in reaction cup by over cleaning liquid reagent needle, cleans to reaction cup；If CRP+SAA
Double projects in reaction cup after discharging of waste liquid, are added cleaning solution the by the cleaning solution reagent needles of the first group reagent needle assemblies
In the reaction cup of one reaction mould group and it is cleaned, is added cleaning solution by the cleaning solution reagent needle of two group reagent needle assemblies
In the reaction cup that second is reacted mould group and it is cleaned.
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| CN113419576A (en) * | 2021-07-08 | 2021-09-21 | 安徽伊普诺康生物技术股份有限公司 | Temperature control system of protein analyzer |
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