CN110244070B - Specific protein analyzer and test method thereof - Google Patents
Specific protein analyzer and test method thereof Download PDFInfo
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- CN110244070B CN110244070B CN201910444735.6A CN201910444735A CN110244070B CN 110244070 B CN110244070 B CN 110244070B CN 201910444735 A CN201910444735 A CN 201910444735A CN 110244070 B CN110244070 B CN 110244070B
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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Abstract
The invention discloses a specific protein analyzer and a test method thereof, the specific protein analyzer comprises a casing and a frame, the frame comprises a front support plate spaced from a front operation plate of the casing and a rear support plate spaced from a back plate of the casing, a two-dimensional arm fixing plate vertical to a bottom plate is arranged between the front support plate and the rear support plate, an X-axis assembly of an automatic two-dimensional arm is arranged on the two-dimensional arm fixing plate, a circuit partition plate spaced from the back plate of the casing is arranged at one side of the two-dimensional arm fixing plate, a control board card is arranged between the circuit partition plate and the casing, a liquid circuit partition plate vertical to the bottom plate is arranged between the circuit partition plate and the front support plate, a horizontal support plate parallel to the bottom plate is arranged between the front support plate and the rear support plate, the horizontal support plate is positioned below one side of the two-dimensional arm fixing plate where the, the reagent storage module comprises at least two reagent storage positions, and the number of the reaction modules is more than two and is even.
Description
Technical Field
The invention relates to a specific protein analyzer and a test method thereof, belonging to the technical field of protein analyzers.
Background
With the continuous progress of the medical level of society, specific protein analyzers have come into wide use in various large and medium hospitals in recent years, and provide essential information basis for the clinical diagnosis, treatment and prevention of diseases and health status.
However, most of the currently mainstream fully-automatic specific protein analyzers are used for measuring inflammation CRP indexes, and with the development of clinical technologies, one CRP index cannot completely meet the requirements, and a more reliable judgment basis can be provided for clinical detection by combining with the SAA index. Meanwhile, the inflammation detection is usually combined with partial indexes of a blood cell analyzer, so that the direct on-machine test of the whole blood sample is supported, the operation of a clinical laboratory technician is simplified, and the time is saved. At present, two different instruments are mostly used for measuring two indexes of CRP and SAA, sample suction for two times not only influences the accuracy of measurement, but also increases the using amount of samples, additionally increases the workload of testers, and in addition, also occupies the instrument placing space of a hospital. The existing CRP + SAA integrated machine is used for analysis and test in a biochemical mode or an immunochromatography mode, and the CRP + SAA integrated machine tested in the biochemical mode has structures such as a reaction disc, a reagent disc and the like, is not compact enough in structure and large in size, and cannot process whole blood; due to the methodology, the testing result of the integrated machine of the immunochromatography mode is greatly influenced by factors such as raw materials, production and the like, and the precision is poor. Therefore, the integrated machine in the prior art has the defects of large volume or low measurement accuracy. At present, a CRP + SAA all-in-one machine which is as small as possible and high in test accuracy is urgently needed in the market.
Disclosure of Invention
The invention aims to provide a specific protein analyzer capable of simultaneously testing CRP and SAA, and aims to solve the problems that the existing CRP + SAA all-in-one machine is large in size and low in testing accuracy. Meanwhile, the invention also provides a test method of the specific protein analyzer.
The invention adopts the following technical scheme: a specific protein analyzer comprises a shell and a frame arranged in the shell, wherein the shell comprises a left side plate, a right side plate, a front operation plate, a back plate and a top plate, the front operation plate is arranged between the left side plate and the right side plate, the front operation plate is provided with a display screen, an output unit and a sample introduction position, the frame comprises a bottom plate, an automatic two-dimensional arm, a reaction module, a reagent needle frame, a reagent storage module, a liquid storage module and a control board are arranged on the frame, the automatic two-dimensional arm comprises an X shaft assembly arranged on the frame and a Y shaft assembly moving back and forth on the X shaft assembly, a swab is arranged at the lower end of the Y shaft assembly, a sampling needle capable of moving up and down is arranged in the swab, a space for the sampling needle to penetrate out is arranged above the sample introduction position on the front operation plate of the shell, the frame comprises a front support plate arranged at a distance from the front, a circuit clapboard which is arranged at a distance from the back plate of the casing is arranged at one side of the two-dimensional arm fixing plate, the control board card is arranged in the area enclosed by the circuit clapboard, the two-dimensional arm fixing plate and the casing, a liquid path clapboard which is vertical to the bottom plate is arranged between the circuit clapboard and the front supporting plate, the liquid storage module is arranged in the area enclosed by the liquid path clapboard, the circuit clapboard, the front supporting plate and the casing, the automatic two-dimensional arm is arranged at the other side of the two-dimensional arm fixing plate, an X shaft component of the automatic two-dimensional arm is arranged on the two-dimensional arm fixing plate, the X shaft component of the automatic two-dimensional arm is horizontally arranged and extends forwards and backwards, a two-dimensional arm perforation which is used for the front end part of the X shaft of the automatic two-dimensional arm and the Y shaft component to penetrate out is arranged on the front, the reagent storage module and the reaction modules are fixed on a horizontal support plate, the reagent storage module comprises at least two reagent storage positions, each reagent storage position is provided with a reagent storage bottle, the number of the reaction modules is more than two, the reaction modules are arranged on the same horizontal plane in the front and back, the reaction bases of the reaction modules extend left and right, the reagent needle frame is connected to a two-dimensional arm fixing plate through a hinge and positioned between the two-dimensional arm and the reaction modules, the reagent needle frame is provided with reagent needle assemblies with the number equal to that of the reaction modules, each reagent needle assembly is correspondingly arranged above each reaction module reaction cup, each reagent needle assembly comprises a reaction liquid reagent needle and a cleaning liquid reagent needle which are connected with the liquid storage module, and the reaction liquid reagent needles and the cleaning liquid reagent needles respectively add cleaning liquid and reaction liquid into the reaction cups through reaction liquid pipelines and cleaning liquid pipelines, the cup mouths of the reaction cups and the bottle mouths of the reagent storage bottles are positioned on the same straight line of the same plane, the straight line and the track formed when the Y-axis assembly of the automatic two-dimensional arm moves horizontally are positioned on the same plane, and the plane is vertical to the horizontal plane.
A gap is formed between the bottom edge of the two-dimensional arm fixing plate and the bottom plate, one end of the reaction base of each reaction module is fixed on the horizontal supporting plate, and the other end of the reaction base penetrates through the gap between the two-dimensional arm fixing plate and the bottom plate.
The reaction module and the reagent storage module are fixed on the bottom surface of the horizontal support plate, and openings are formed in the positions, corresponding to the cup mouths of the reaction cups and the bottle mouths of the reagent bottles, of the horizontal support plate.
Circuit baffle and liquid way baffle all are located two-dimentional arm fixed plate left side position, horizontal support plate is located two-dimentional arm fixed plate right side position, and the back backup pad is located the right side position of two-dimentional arm fixed plate rear end.
The last agitator motor of reaction module is located reaction base upper reaction cup right side position, and the last optical channel of reaction module is located reaction base upper reaction cup left side position, and agitator motor's output shaft level sets up, is connected with the magnet carousel that the axis level set up on the output shaft, and the magnet carousel is close to the reaction cup lower part, is equipped with the no magnetism stirring rod of rotatory under the drive of magnet carousel in the reaction cup.
The reagent storage module comprises a refrigeration base, a refrigeration outer cover fixed on the refrigeration base, and a refrigeration inner core and a semiconductor refrigeration piece which are arranged in the refrigeration outer cover, wherein heat preservation cotton is arranged between the refrigeration outer cover and the refrigeration inner core, the reagent storage position is arranged in the refrigeration inner core, the refrigeration inner core is tightly attached to the refrigeration surface of the refrigeration piece, the heating surface of the semiconductor refrigeration piece is provided with a cooling fin positioned outside the refrigeration outer cover, and one side, away from the refrigeration outer cover, of the cooling fin is provided with a cooling fan.
The reaction module is characterized in that the number of the reaction modules is four, the number of the reagent needle assemblies is four, and each group of the reagent needle assemblies is arranged above the reaction cup of each reaction module.
The bottom plate, the front supporting plate, the rear supporting plate, the two-dimensional arm fixing plate, the circuit partition plate, the liquid path partition plate and the horizontal supporting plate are all sheet metal machined parts.
An assay method for performing an assay using a specific protein analyzer, comprising the steps of: (1) selecting a test item as a single CRP or single SAA or CRP + SAA double item; (2) loading a sample to be detected into a test tube, and then placing the test tube at a sample injection position on an operation plate in front of a machine shell; (3) the Y-axis assembly drives the sampling needle to move by adjusting the two-dimensional arm, and the sampling needle penetrates out of the front operation plate of the shell to a sample introduction position to quantitatively suck samples in the test tube; (4) adding the reaction liquid in the liquid storage module into a reaction cup of the corresponding reaction module through a reaction liquid reagent needle; if the CRP + SAA project is the two projects, adding the reaction liquid into the reaction cup of the first reaction module through the reaction liquid reagent needles of the first group of reagent needle assemblies, and adding the reaction liquid into the reaction cup of the second reaction module through the reaction liquid reagent needles of the two groups of reagent needle assemblies; (5) inserting a sampling needle into the reaction cup of the reaction module to which the reaction liquid is added by adjusting the two-dimensional arm, adding a sample sucked by the sampling needle into the reaction cup, and cleaning the sampling needle by a swab; if the CRP + SAA double items exist, sampling twice by using a sampling needle, respectively adding the samples sucked twice into a reaction cup of a first reaction module and a reaction cup of a second reaction module, or sucking once by using the sampling needle, sequentially adding the sucked samples into the reaction cup of the first reaction module and the reaction cup of the second reaction module, and cleaning the sampling needle by using a swab after the sample is added; (6) starting a stirring motor to uniformly mix the reaction liquid and the sample in the group reaction cup; (7) adjusting the two-dimensional arm, moving the sampling needle to the reagent storage module, and quantitatively sucking the reagent in the reagent storage bottle by the sampling needle; (8) moving the sampling needle into a reaction cup added with reaction liquid and a sample, and inserting the sampling needle into the reaction cup to add a quantitative reagent; if the CRP + SAA double items exist, a sampling needle firstly quantitatively sucks a reagent in a first reagent storage bottle, the reagent is added into a reaction cup of a first reaction module, the sampling needle is cleaned and then quantitatively sucks a reagent in another reagent storage bottle, the reagent is added into a reaction cup of a second reaction module, and the sampling needle is cleaned after each reagent addition; (9) uniformly mixing the reaction solution, the sample and the reagent in the reaction cup by a stirring motor; (10) reacting and measuring the sample through the reaction module; (11) transmitting a measurement signal through a control board card, converting the signal and calculating a final result; (12) the detection result is displayed through a display screen on the shell, and the final result is output through an output unit on the shell; (13) discharging the waste liquid after reaction in the reaction cup to a waste liquid barrel outside the shell, adding the cleaning liquid in the liquid storage module into the reaction cup through a cleaning liquid reagent needle, and cleaning the reaction cup; if the CRP + SAA project is adopted, after waste liquid in the reaction cup is discharged, cleaning liquid is added into the reaction cup of the first reaction module through the cleaning liquid reagent needles of the first group of reagent needle assemblies and is cleaned, and cleaning liquid is added into the reaction cup of the second reaction module through the cleaning liquid reagent needles of the two groups of reagent needle assemblies and is cleaned.
The invention has the beneficial effects that: in the protein analyzer, the front support plate, the rear support plate, the two-dimensional arm fixing plate, the circuit partition plate and the liquid path partition plate in the rack are arranged into a structure similar to a II shape, so that the two-dimensional arm, the control board card and the liquid path assembly are arranged in different areas, and the two-dimensional arm, the reagent needle holder and the reaction module are sequentially arranged from top to bottom through the horizontal support plate, therefore, the layout mode has the following advantages: (1) all parts are compactly distributed on the rack in the casing, the utilization of the internal space of the casing is maximized, and the instrument placing space is ensured to be also minimized; (2) the control board card and the liquid path assembly are positioned in different spaces through the circuit partition board and the liquid path partition board, so that the liquid path and the control board card are completely separated and placed, the circuit is prevented from being damaged when the liquid path breaks down, and short circuit is avoided; (3) the reagent needle assembly provided by the invention is provided with two reagent needles, one-time sample suction can simultaneously meet the test requirements of two items of CRP + SAA, and the consumption of samples is extremely low.
Preferably, a gap is formed between the bottom edge of the two-dimensional arm fixing plate and the bottom plate, so that the reaction module can conveniently pass through the gap.
Preferably, the horizontal support plate is located at the right side of the two-dimensional arm fixing plate, and the corresponding sample injection position is located at the right side of the machine shell to be tested, so that the machine shell meets the requirements of human-machine engineering.
The preferred adopts the design of reaction module, and high, the water-proof effects of utilization ratio to optics is good, the fault rate is less than the mainstream model in the market, and the stirring rod does not have magnetism, can avoid the stirring rod adhesion to cause the puzzlement for the user in some places that are difficult for noticing like this, and the design of side stirring can also be solved and is stirred the unusual of in-process stirring rod departure reaction cup.
Preferably, the number of reaction modules is four, and the number of reagent needle assemblies is four.
Drawings
FIG. 1 is a complete external view of a specific protein analyzer according to one embodiment of the present invention;
FIG. 2 is an internal layout of the specific protein analyzer of FIG. 1;
FIG. 3 is a view 1 of the internal structure of the specific protein analyzer of FIG. 1;
FIG. 4 is a view 2 of the internal structure of the specific protein analyzer of FIG. 1 (reagent pack turned up);
FIG. 5 is a top view of FIG. 3;
FIG. 6 is a schematic diagram of the robotic two-dimensional arm of FIG. 3;
FIG. 7 is a schematic view of the reaction module shown in FIG. 3;
FIG. 8 is a cross-sectional view of the reaction module 1 of FIG. 3;
FIG. 9 is a cross-sectional view of the reaction module 2 of FIG. 3;
FIG. 10 is a schematic diagram of the reagent storage module of FIG. 3;
FIG. 11 is a cross-sectional view of the reagent storage module of FIG. 3;
FIG. 12 is a schematic view of the robotic two-dimensional arm, reaction module, and reagent needle holder of FIG. 3;
FIG. 13 is a flow chart of a method of testing a specific protein analyzer of the present invention.
In the figure, 100-machine shell, 101-display screen, 102-switch, 103-output unit, 104-sample introduction position, 200-machine frame, 201-two-dimensional arm fixing plate, 202-liquid path clapboard, 203-circuit clapboard, 204-front supporting plate, 205-rear supporting plate, 206-horizontal supporting plate, 207-bottom plate, 300-reagent needle holder, 301-reagent needle assembly, 302-reaction liquid reagent needle, 303-cleaning liquid reagent needle, 400-two-dimensional arm, 401-X shaft assembly, 402-Y shaft assembly, 403-sampling needle, 404-swab, 405-two-dimensional arm bracket, 500-reaction module, 501-reaction base, 502-heating cup, 503-reaction cup, 504-laser, 505-photoelectric receiving plate, 506-a non-magnetic stirring rod, 507-a stirring motor, 508-a magnet turntable, 509-a liquid outlet, 800-a reagent storage module, 801-a refrigeration base, 802-a refrigeration outer cover, 803-a refrigeration inner core, 804-heat insulation cotton, 805-a semiconductor refrigeration sheet, 806-a heat radiating sheet, 807-a fan, 808-a temperature sensor and 809-a reagent storage box.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments.
Fig. 1 to 12 show a specific protein analyzer according to an embodiment of the present invention, which includes a housing 100 and a rack 200 disposed in the housing 100.
The rack 200 comprises a bottom plate 207, the rack 200 is provided with an automatic two-dimensional arm 400, a reaction module 500, a reagent needle rack 300, a reagent storage module 800, a liquid storage module and a control board card, and the plane layout of all the components on the rack 200 is shown in fig. 2. The liquid storage module is connected with corresponding parts through a liquid path assembly, and the liquid path assembly comprises a plunger pump, a diaphragm pump, an electromagnetic valve and the like.
The structure of the automatic two-dimensional arm 400 is as shown in fig. 6, the automatic two-dimensional arm 400 includes an X-axis assembly 401 mounted on the rack and a Y-axis assembly 402 moving back and forth on the X-axis assembly 401, a swab 404 is disposed at the lower end of the Y-axis assembly 402, a sampling needle 403 capable of moving up and down is disposed in the swab 404, a space for the sampling needle to penetrate out is disposed above the sampling position 104 on the front operation board of the housing, the structure of the automatic two-dimensional arm 400 is the same as that of the prior art, the X-axis assembly 401 and the Y-axis assembly 402 both use a linear motion design mode of a guide rail and a synchronous. The movement in the horizontal direction has seven stopping positions in total, the seven stopping positions are controlled by seven optical couplers respectively, the seven optical couplers are connected by independent lines, the influence of step motor step loss is avoided, and the movement accuracy is improved. On the Y axle subassembly 402, sampling needle 403 can be the up-and-down motion under the drive of Y axle hold-in range, and swab 404 links to each other with the liquid circuit through two rubber tubes, can realize through the control of pump valve that the inside and outside wall of sampling needle 403 washs, and sampling needle 403 not only is used for the suction appearance application of sample, but also can absorb the reagent in the reagent bottle to add in reaction cup 503. Need carry out inside and outside wall washing to sampling needle 403 at the in-process that sampling needle 403 inhaled different liquid to guarantee that cross contamination can not appear, specific operation flow is: through the unique time sequence control design, the cleaning effect on the inner wall and the outer wall of the sampling needle 403 is excellent, and the clinical automatic two-dimensional arm 400 is arranged on a rack through the two-dimensional arm support 405.
As shown in fig. 3 to 5, the rack 200 includes a front support plate 204 spaced apart from a front operation plate of the housing 100, a rear support plate 205 spaced apart from a back plate of the housing 100, a two-dimensional arm fixing plate 201 perpendicular to the bottom plate 207 is disposed between the front support plate 204 and the rear support plate 205, a circuit partition 203 spaced apart from the back plate of the housing 100 is disposed on one side of the two-dimensional arm fixing plate 201, a control board is mounted in an area enclosed by the circuit partition 203, the two-dimensional arm fixing plate 201 and the housing 100, a liquid partition 202 perpendicular to the bottom plate is disposed between the circuit partition 203 and the front support plate 204, the liquid storage module is mounted in an area enclosed by the liquid partition 202, the circuit partition 203, the front support plate 204 and the housing 100, the automatic two-dimensional arm 400 is mounted on the other side of the two-dimensional arm fixing plate 201, an X-axis assembly 401 of the automatic two-dimensional arm 400 is mounted on, the automatic two-dimensional arm comprises a front support plate 204, a two-dimensional arm through hole is formed in the front support plate 204, the front end portion of an X shaft 401 of the automatic two-dimensional arm and the two-dimensional arm through hole penetrates through a Y shaft assembly 402 of the automatic two-dimensional arm, a horizontal support plate 206 which is parallel to a bottom plate 207 is arranged between the front support plate 204 and a rear support plate 205, the horizontal support plate 206 is located at a position below one side, provided with an automatic two-dimensional arm 400, of a two-dimensional arm fixing plate 201, a reagent storage module 800 and a reaction module 500 are fixed on the horizontal support plate 206, the reagent storage module 800 comprises at least two reagent storage positions 809, each reagent storage position is internally provided with a reagent storage bottle 809, the number of the reaction modules 500 is more than two. The bottom plate 207, the front support plate 204, the rear support plate 205, the two-dimensional arm fixing plate 201, the circuit partition plate 203, the liquid path partition plate 202 and the horizontal support plate 206 are all sheet metal workpieces.
As shown in fig. 12, the reagent needle holder 300 is hinged to the two-dimensional arm fixing plate 201 at a position between the automatic two-dimensional arm 400 and the reaction module 500, the reagent needle rack 300 is provided with reagent needle assemblies 301 with the same number as the reaction modules 500, each reagent needle assembly 301 is correspondingly arranged above the reaction cup 503 of each reaction module 500, each reagent needle assembly 301 comprises a reaction liquid reagent needle 302 and a cleaning liquid reagent needle 303 which are connected with the liquid storage module, the reaction liquid reagent needle 302 and the cleaning liquid reagent needle 303 respectively add the cleaning liquid and the reaction liquid into the reaction cup 503 through a reaction liquid pipeline and a cleaning liquid pipeline, the cup mouths of the reaction cups 503 and the bottle mouths of the reagent storage bottles are positioned on the same straight line of the same plane, the straight line is on a plane with the trajectory formed by the horizontal motion of the Y-axis assembly 402 of the robotic two-dimensional arm, and the plane is perpendicular to the horizontal plane. After a test is completed on one reaction cup 503, the waste liquid in the reaction cup 503 is discharged into the waste liquid barrel of the liquid storage module through the liquid path pipeline where the waste liquid is located, the cleaning liquid reagent needle 303 above the reaction cup 503 injects a proper amount of cleaning liquid into the reaction cup 503 to clean the reaction cup, then the waste liquid is discharged into the waste liquid barrel of the liquid storage module, and the process is repeated for 2-3 times to complete the cleaning of the reaction cup 503. After the completion of the cleaning, a certain amount of reaction solution is injected into the cuvette 503 through the reaction solution reagent needle 302 above the cuvette 503, and the start of the next test is waited. Only a brief overview of cup cleaning is provided here, and is described in more detail in the following test procedures.
In this embodiment, the number of the reaction module 500 is four, and the reagent needle assemblies 301 are also four, and each group of the reagent needle assemblies 301 is disposed above the reaction cup of each reaction module 500. The reagent needle of the reaction liquid and the reagent needle of the cleaning liquid in each reagent needle assembly 301 are respectively connected with the liquid storage module through pipelines and are respectively used for adding the reaction liquid and the cleaning liquid into the reaction cup 503, the reagent needle of the reaction liquid and the reagent needle of the cleaning liquid are independent and do not interfere with each other, the reagent needle frame 301 is fixed on the two-dimensional arm fixing plate through a hinge, and the reagent needle frame 301 can drive the two reagent needles to turn up together so as to replace the reaction cup 503. The four reaction modules 500 are tested independently, so that cross contamination among the reaction modules is avoided, and when a certain channel fails, the instrument can still normally test.
A gap is formed between the bottom edge of the two-dimensional arm fixing plate 201 and the bottom plate 207, and one end of the reaction base 501 of each reaction module 500 is fixed on the horizontal support plate 206, and the other end passes through the gap between the two-dimensional arm fixing plate 201 and the bottom plate 207.
The reaction module 500 and the reagent storage module 800 are both fixed on the bottom surface of the horizontal support plate 206, and openings are provided on the horizontal support plate 206 corresponding to the positions of the mouths of the reaction cups and the mouths of the reagent bottles.
The reaction module is structured as shown in fig. 7 to fig. 9, a stirring motor 507 on the reaction module 500 is located at the right side of a reaction cup 503 on a reaction base 501, an optical channel on the reaction module 500 is located at the left side of the reaction cup on the reaction base 501, a transparent reaction cup 503 is arranged on the reaction base opposite to the stirring motor, a heating cup 502 is arranged below the reaction cup 503, electronic components such as a heating rod and a temperature sensor are installed in the heating cup 502 for heating and incubating liquid in the reaction cup 503, the stirring motor 507 is located at the lower right side of the heating cup 502, an output shaft of the stirring motor 507 is horizontally arranged, a magnet turntable 508 with a horizontally arranged axis is connected to the output shaft, the magnet turntable 508 is close to the lower portion of the reaction cup, and a non-magnetic stirring rod 506 which is driven by the magnet turntable 508 to rotate is arranged in the reaction. The magnetic field of the magnet turntable 508 drives the stirring rod 506 to rotate together, so as to achieve the purpose of uniformly mixing the liquid in the reaction cup 502. The reaction light source and the photoelectric receiving sensor are respectively arranged at the front end and the rear end of the reaction base, a walking route of the reaction light source in the reaction base forms a certain included angle with the horizontal direction, a filter is connected with a rubber tube below the reaction base, and the rubber tube is connected with the liquid storage module below the filter. Different from the design idea of placing the stirring motor below the reaction module in the conventional machine type in the prior art, the stirring motor 507 is placed on the side surface of the reaction module 500 in the embodiment, and the stirring rod 506 is not required to have magnetism, so that the trouble caused by the adhesion of the stirring rod 506 to some places which are not easy to perceive for users can be avoided, and the abnormity that the stirring rod 506 flies out of the reaction cup 503 in the stirring process can be solved by the design of side stirring. The rear side of the reaction base 501 is provided with a laser 504 as a reaction light source, the front side of the reaction base 501 is provided with a photoelectric receiving plate 505 as a photoelectric receiving sensor, and the laser 504 and the photoelectric receiving plate 505 realize the detection of the sample by using a nephelometry method. After a test is completed, the waste liquid in the reaction cup 503 will pass through the liquid outlet 509 and be discharged into the waste liquid barrel of the liquid storage module through the liquid path.
The reagent storage module has a refrigeration function, as shown in fig. 10 to 11, the reagent storage module 800 includes a refrigeration base 801, a refrigeration cover 802 fixed on the refrigeration base 801, and a refrigeration core 803 and a semiconductor refrigeration sheet 805 arranged in the refrigeration cover 802, the refrigeration base 801 and the refrigeration cover 802 are connected by screws, a heat insulation cotton 804 is arranged between the refrigeration cover 802 and the refrigeration core 803, and reagent storage locations 809 are arranged in the refrigeration core 803. The refrigeration inner core 803 is tightly attached to the refrigeration surface of the semiconductor refrigeration sheet 805, the heating surface of the semiconductor refrigeration sheet 805 is attached with a heat radiation fin 806 positioned outside the refrigeration outer cover 801, and one side of the heat radiation fin 806 far away from the refrigeration outer cover is provided with a heat radiation fan 807. The heat sink 806 is screwed to the refrigeration shell 802 and the refrigeration core 803 also has a temperature sensor 808 mounted to the bottom. When the reagent storage module 800 is in an operating state, the refrigerating surface of the semiconductor refrigerating plate 805 refrigerates the refrigerating core 803, and the heat dissipation fan 807 dissipates heat of the semiconductor refrigerating plate 805 by matching with the heat dissipation plate 806, so as to ensure normal operation of the semiconductor refrigerating plate. The temperature sensor 808 is used in conjunction with a temperature control board (not shown) located in the area of the control board behind the instrument to ensure that the temperature on the cooling base 801 reaches a predetermined temperature. The two reagent storage sites 809 can simultaneously store two latex reagents of CRP and SAA, and two tests of CRP and SAA can be carried out by one-time sample suction.
The specific protein analyzer can operate the apparatus through the touch display screen 101, can automatically complete a series of operations such as sample suction, test, cleaning and the like, and is convenient and quick; according to the rack for specific protein analysis, the liquid storage module and the control board card are separated from each other, so that the influence on a circuit caused by a liquid path fault is avoided, and the high-integration layout design enables the routing pipes and the routing wires to be more orderly and is convenient to maintain; the four independent reaction modules of the specific protein analyzer can independently complete the test, even if a certain reaction module breaks down, the normal use of the analyzer is not influenced, and the modularized design is convenient for maintenance and production; the reagent storage module of the specific protein analyzer provided by the invention is provided with two reagent storage positions, can simultaneously store two latex reagents of CRP and SAA, and can perform two tests of CRP and SAA by one-time sample suction.
The invention has the following beneficial effects: (1) the layout is compact, the space utilization is maximized, and the instrument placing space is ensured to be the minimum; (2) the liquid storage module and the control board are completely isolated, so that the circuit is prevented from being damaged when a liquid path fails; (3) the sample suction for one time simultaneously meets the test of two items of CRP and SAA, and the consumption of the sample reaches an extremely low limit; (4) the innovative reaction module design has high optical utilization rate, good waterproof effect and low failure rate than the main machine type in the market.
In a specific embodiment of the analysis method performed by the specific protein analyzer of the present invention, the flow of the analysis method of this embodiment is shown in fig. 13, and the method includes the following steps: (1) selecting a test item as a single CRP or single SAA or CRP + SAA double item; (2) loading a sample to be detected into a test tube, and then placing the test tube at a sample injection position on an operation plate in front of a machine shell; (3) the Y-axis assembly drives the sampling needle to move by adjusting the two-dimensional arm, and the sampling needle penetrates out of the front operation plate of the shell to a sample introduction position to quantitatively suck samples in the test tube; (4) adding the reaction liquid in the liquid storage module into a reaction cup of the corresponding reaction module through a reaction liquid reagent needle; if the CRP + SAA project is the two projects, adding the reaction liquid into the reaction cup of the first reaction module through the reaction liquid reagent needles of the first group of reagent needle assemblies, and adding the reaction liquid into the reaction cup of the second reaction module through the reaction liquid reagent needles of the two groups of reagent needle assemblies; (5) inserting a sampling needle into the reaction cup of the reaction module to which the reaction liquid is added by adjusting the two-dimensional arm, adding a sample sucked by the sampling needle into the reaction cup, and cleaning the sampling needle by a swab; if the CRP + SAA double items exist, sampling twice by using a sampling needle, respectively adding the samples sucked twice into a reaction cup of a first reaction module and a reaction cup of a second reaction module, or sucking once by using the sampling needle, sequentially adding the sucked samples into the reaction cup of the first reaction module and the reaction cup of the second reaction module, and cleaning the sampling needle by using a swab after the sample is added; (6) starting a stirring motor to uniformly mix the reaction liquid and the sample in the group reaction cup; (7) adjusting the two-dimensional arm, moving the sampling needle to the reagent storage module, and quantitatively sucking the reagent in the reagent storage bottle by the sampling needle; (8) moving the sampling needle into a reaction cup added with reaction liquid and a sample, and inserting the sampling needle into the reaction cup to add a quantitative reagent; if the CRP + SAA double items exist, a sampling needle firstly quantitatively sucks a reagent in a first reagent storage bottle, the reagent is added into a reaction cup of a first reaction module, the sampling needle is cleaned and then quantitatively sucks a reagent in another reagent storage bottle, the reagent is added into a reaction cup of a second reaction module, and the sampling needle is cleaned after each reagent addition; (9) uniformly mixing the reaction solution, the sample and the reagent in the reaction cup by a stirring motor; (10) reacting and measuring the sample through the reaction module; (11) transmitting a measurement signal through a control board card, converting the signal and calculating a final result; (12) the detection result is displayed through a display screen on the shell, and the final result is output through an output unit on the shell; (13) discharging the waste liquid after reaction in the reaction cup to a waste liquid barrel outside the shell, adding the cleaning liquid in the liquid storage module into the reaction cup through a cleaning liquid reagent needle, and cleaning the reaction cup; if the CRP + SAA project is adopted, after waste liquid in the reaction cup is discharged, cleaning liquid is added into the reaction cup of the first reaction module through the cleaning liquid reagent needles of the first group of reagent needle assemblies and is cleaned, and cleaning liquid is added into the reaction cup of the second reaction module through the cleaning liquid reagent needles of the two groups of reagent needle assemblies and is cleaned.
The above embodiments are only related to the main improvement points of the present invention, and the parts of the present invention not mentioned or described in detail are the same as the prior art, such as the principle of optical test, the structure of the control board, the specific structure of the liquid storage module, the circuit and the principle of liquid circuit control, etc., so the present invention is not described in detail.
The above description is only for the preferred embodiment of the present invention, and the above specific embodiments are not intended to limit the present invention. Various modifications and alterations may occur to those skilled in the art without departing from the spirit and scope of the invention, and such modifications, alterations, and substitutions may be made without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (8)
1. The utility model provides a specific protein analyzer, including casing and the frame of setting in the casing, the casing includes two curb plates of left and right sides, preceding operation panel and backplate and the roof of setting between the board of left and right sides, be equipped with the display screen on the preceding operation panel, output unit, and advance kind of the position, the frame includes the bottom plate, install automatic two dimension arm in the frame, the reaction module, reagent needle frame, the module is deposited to the reagent, stock solution module and control integrated circuit board, automatic two dimension arm is including installing the X axle subassembly in the frame and the Y axle subassembly at X axle subassembly round trip movement, Y axle subassembly lower extreme is equipped with the swab, be equipped with the sampling needle that can reciprocate in the swab, it has the space that supplies the sampling needle to wear out to lie in advance kind of position on: the machine frame comprises a front supporting plate and a rear supporting plate, the front supporting plate and the rear supporting plate are arranged at intervals, the rear supporting plate is arranged at intervals with a back plate of the machine case, a two-dimensional arm fixing plate perpendicular to a bottom plate is arranged between the front supporting plate and the rear supporting plate, a circuit partition plate is arranged at one side of the two-dimensional arm fixing plate and is arranged at intervals with the back plate of the machine case, a control board card is arranged in an area enclosed by the circuit partition plate, the two-dimensional arm fixing plate and the machine case, a liquid circuit partition plate perpendicular to the bottom plate is arranged between the circuit partition plate and the front supporting plate, a liquid storage module is arranged in the area enclosed by the liquid circuit partition plate, the front supporting plate and the machine case, an automatic two-dimensional arm is arranged at the other side of the two-dimensional arm fixing plate, an X shaft assembly of the automatic two-dimensional arm is arranged on the, a horizontal support plate arranged in parallel with the bottom plate is arranged between the front support plate and the rear support plate, the horizontal support plate is positioned at the position below one side of the two-dimensional arm fixing plate, which is provided with an automatic two-dimensional arm, a gap is arranged between the bottom edge of the two-dimensional arm fixing plate and the bottom plate, one end of each reaction module reaction base is fixed on the horizontal support plate, the other end of each reaction module reaction base penetrates through the gap between the two-dimensional arm fixing plate and the bottom plate, the reagent storage module and the reaction modules are fixed on the horizontal support plate, the reagent storage module comprises at least two reagent storage positions, each reagent storage position is provided with a reagent storage bottle, the number of the reaction modules is more than two, each reaction module is arranged on the same horizontal plane in the front and back direction, the reaction bases of each reaction module extend in the left and right directions, and the reagent needle frame, the reagent needle rack is provided with reagent needle assemblies with the same number as the reaction modules, each reagent needle assembly is correspondingly arranged above each reaction module reaction cup, each reagent needle assembly comprises a reaction liquid reagent needle and a cleaning liquid reagent needle which are connected with the liquid storage module, the reaction liquid reagent needle and the cleaning liquid reagent needle respectively add reaction liquid and cleaning liquid into each reaction cup through a reaction liquid pipeline and a cleaning liquid pipeline, cup mouths of each reaction cup and bottle mouths of each reagent storage bottle are located on the same straight line of the same plane, the straight line and a track formed when a Y shaft assembly of the automatic two-dimensional arm moves horizontally are located on the same plane, the plane is perpendicular to the horizontal plane, the reagent needle rack comprises a pressing plate for pressing the cup mouths of the reaction cups, and the reaction liquid reagent needles and the cleaning liquid reagent needles all penetrate through the pressing plate.
2. The specific protein analyzer as set forth in claim 1, wherein: the reaction module and the reagent storage module are fixed on the bottom surface of the horizontal support plate, and openings are formed in the positions, corresponding to the cup mouths of the reaction cups and the bottle mouths of the reagent bottles, of the horizontal support plate.
3. The specific protein analyzer as set forth in claim 1, wherein: circuit baffle and liquid way baffle all are located two-dimentional arm fixed plate left side position, horizontal support plate is located two-dimentional arm fixed plate right side position, and the back backup pad is located the right side position of two-dimentional arm fixed plate rear end.
4. The specific protein analyzer according to claim 3, wherein: the last agitator motor of reaction module is located reaction base upper reaction cup right side position, and the last optical channel of reaction module is located reaction base upper reaction cup left side position, and agitator motor's output shaft level sets up, is connected with the magnet carousel that the axis level set up on the output shaft, and the magnet carousel is close to the reaction cup lower part, is equipped with the no magnetism stirring rod of rotatory under the drive of magnet carousel in the reaction cup.
5. The specific protein analyzer as set forth in claim 1, wherein: the reagent storage module comprises a refrigeration base, a refrigeration outer cover fixed on the refrigeration base, and a refrigeration inner core and a semiconductor refrigeration piece which are arranged in the refrigeration outer cover, wherein heat preservation cotton is arranged between the refrigeration outer cover and the refrigeration inner core, the reagent storage position is arranged in the refrigeration inner core, the refrigeration inner core is tightly attached to the refrigeration surface of the refrigeration piece, the heating surface of the semiconductor refrigeration piece is provided with a cooling fin positioned outside the refrigeration outer cover, and one side, away from the refrigeration outer cover, of the cooling fin is provided with a cooling fan.
6. The specific protein analyzer as set forth in claim 1, wherein: the reaction module is characterized in that the number of the reaction modules is four, the number of the reagent needle assemblies is four, and each group of the reagent needle assemblies is arranged above the reaction cup of each reaction module.
7. The specific protein analyzer as set forth in claim 1, wherein: the bottom plate, the front supporting plate, the rear supporting plate, the two-dimensional arm fixing plate, the circuit partition plate, the liquid path partition plate and the horizontal supporting plate are all sheet metal machined parts.
8. An analysis method for analysis using the specific protein analyzer of claim 1, characterized in that it comprises the steps of: (1) selecting a test item as a single CRP or single SAA or CRP + SAA double item; (2) loading a sample to be detected into a test tube, and then placing the test tube at a sample injection position on an operation plate in front of a machine shell; (3) the Y-axis assembly drives the sampling needle to move by adjusting the two-dimensional arm, and the sampling needle penetrates out of the front operation plate of the shell to a sample introduction position to quantitatively suck samples in the test tube; (4) adding the reaction liquid in the liquid storage module into a reaction cup of the corresponding reaction module through a reaction liquid reagent needle; if the CRP + SAA project is the two projects, adding the reaction liquid into the reaction cup of the first reaction module through the reaction liquid reagent needles of the first group of reagent needle assemblies, and adding the reaction liquid into the reaction cup of the second reaction module through the reaction liquid reagent needles of the two groups of reagent needle assemblies; (5) inserting a sampling needle into the reaction cup of the reaction module to which the reaction liquid is added by adjusting the two-dimensional arm, adding a sample sucked by the sampling needle into the reaction cup, and cleaning the sampling needle by a swab; if the CRP + SAA double items exist, sampling twice by using a sampling needle, respectively adding the samples sucked twice into a reaction cup of a first reaction module and a reaction cup of a second reaction module, or sucking once by using the sampling needle, sequentially adding the sucked samples into the reaction cup of the first reaction module and the reaction cup of the second reaction module, and cleaning the sampling needle by using a swab after the sample is added; (6) starting a stirring motor to uniformly mix the reaction liquid and the sample in the group reaction cup; (7) adjusting the two-dimensional arm, moving the sampling needle to the reagent storage module, and quantitatively sucking the reagent in the reagent storage bottle by the sampling needle; (8) moving the sampling needle into a reaction cup added with reaction liquid and a sample, and inserting the sampling needle into the reaction cup to add a quantitative reagent; if the CRP + SAA double items exist, a sampling needle firstly quantitatively sucks a reagent in a first reagent storage bottle, the reagent is added into a reaction cup of a first reaction module, the sampling needle is cleaned and then quantitatively sucks a reagent in another reagent storage bottle, the reagent is added into a reaction cup of a second reaction module, and the sampling needle is cleaned after each reagent addition; (9) uniformly mixing the reaction solution, the sample and the reagent in the reaction cup by a stirring motor; (10) reacting and measuring the sample through the reaction module; (11) transmitting a measurement signal through a control board card, converting the signal and calculating a final result; (12) the detection result is displayed through a display screen on the shell, and the final result is output through an output unit on the shell; (13) discharging the waste liquid after reaction in the reaction cup to a waste liquid barrel outside the shell, adding the cleaning liquid in the liquid storage module into the reaction cup through a cleaning liquid reagent needle, and cleaning the reaction cup; if the CRP + SAA project is adopted, after waste liquid in the reaction cup is discharged, cleaning liquid is added into the reaction cup of the first reaction module through the cleaning liquid reagent needles of the first group of reagent needle assemblies and is cleaned, and cleaning liquid is added into the reaction cup of the second reaction module through the cleaning liquid reagent needles of the two groups of reagent needle assemblies and is cleaned.
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