CN111693354A - Frozen slice immunohistochemical staining machine and method - Google Patents
Frozen slice immunohistochemical staining machine and method Download PDFInfo
- Publication number
- CN111693354A CN111693354A CN202010764103.0A CN202010764103A CN111693354A CN 111693354 A CN111693354 A CN 111693354A CN 202010764103 A CN202010764103 A CN 202010764103A CN 111693354 A CN111693354 A CN 111693354A
- Authority
- CN
- China
- Prior art keywords
- assembly
- frozen
- dyeing
- reagent bottle
- immunohistochemical staining
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000011532 immunohistochemical staining Methods 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 40
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 93
- 238000004043 dyeing Methods 0.000 claims abstract description 56
- 239000011521 glass Substances 0.000 claims abstract description 38
- 238000001514 detection method Methods 0.000 claims abstract description 20
- 230000002055 immunohistochemical effect Effects 0.000 claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 14
- 210000000056 organ Anatomy 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 46
- 238000006243 chemical reaction Methods 0.000 claims description 23
- 238000010186 staining Methods 0.000 claims description 20
- 238000005406 washing Methods 0.000 claims description 15
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 230000003197 catalytic effect Effects 0.000 claims description 5
- 238000009434 installation Methods 0.000 claims description 4
- 239000003550 marker Substances 0.000 claims description 3
- 238000003364 immunohistochemistry Methods 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 9
- 238000010827 pathological analysis Methods 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 description 9
- 238000003745 diagnosis Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 4
- 230000008014 freezing Effects 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000005574 cross-species transmission Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00138—Slides
Abstract
The invention relates to a frozen slice immunohistochemical staining machine and a frozen slice immunohistochemical staining method. The frozen slice immunohistochemical dyeing machine is provided with N (N is not less than 2) dyeing square bins, wherein each dyeing square bin comprises a reagent bottle assembly, a slide glass assembly, a loading needle assembly, a mechanical arm and a heating assembly; each dyeing square bin can independently complete immunohistochemical dyeing work. Frozen sections of an organ (tissue) are combined into a section assembly, reagent bottles matched with detection molecules of the section assembly are assembled into a reagent bottle assembly, and a group of sections are simultaneously subjected to sample adding and detection, so that the time of the whole experimental process is shortened. By the aid of the frozen section dyeing machine and the immunohistochemical dyeing method based on the frozen section dyeing machine, frozen sections sequentially delivered to specimens can be quickly immunohistochemically dyed in time, and accordingly accuracy of pathological diagnosis of the frozen sections is improved.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a frozen slice immunohistochemical staining machine and a frozen slice immunohistochemical staining method.
Background
The quick frozen section examination in the operation is that an operating doctor requests a pathologist to perform urgent consultation on the disease diagnosis problem related to an operation scheme in the operation process, is the most important and difficult work in clinical pathological work, has the characteristics of high technology, high difficulty and high risk, and mainly has the advantages that the time is urgent, the doctor needs to issue a report within 30 minutes, and the other important reason is that the frozen section diagnosis lacks technical support such as immunohistochemical staining.
The existing immunohistochemical staining equipment and method cannot perform rapid immunohistochemical staining on the frozen section, so that the frozen section can be diagnosed more accurately.
The reasons include: an immunohistochemical dyeing machine only has one dyeing functional area, and can not detect successively-inspected specimens on the same equipment in time; the mechanical arm moves in an X/Y/Z freedom degree motion mode, and the action time is long; generally, a sample adding needle is adopted to sequentially add samples to each slice, and the same molecules to be detected on different slides are detected by using reagents in the same reagent bottle, so that the test process period is long; the installation of the reagent bottle and the placement of the slide are not optimized into modules which are convenient to install/disassemble, so that the use is time-consuming and inconvenient; the glass slide only has a mode of fixing a position in a dyeing machine, and cannot meet the requirements of a sampling reaction mode and a washing mode in the actual dyeing process, so that the reaction and washing efficiency is low, the time is prolonged, and the quality is reduced; the dyeing method in the automatic immunohistochemical dyeing machine is characterized in that catalytic enzyme is marked on a second antibody, during detection, after an antigen is identified and combined through the first antibody, the second antibody is combined with the first antibody, a catalytic enzyme on the second antibody is used for catalyzing a substrate to develop color, and the immunohistochemical dyeing is carried out by adopting the two-step method, so that the immunohistochemical dyeing time is prolonged, the factors cause the long whole flow period and low efficiency of the existing automatic immunohistochemical dyeing machine, and the automatic immunohistochemical dyeing technology cannot be applied to frozen slice diagnosis.
Disclosure of Invention
The invention aims to provide a freezing immunohistochemical staining machine and a freezing immunohistochemical staining method. Mainly relates to equipment and a method capable of carrying out rapid immunohistochemical staining on a clinical frozen section, thereby improving the quality of clinical frozen section diagnosis and reducing the diagnosis difficulty and the risk of misdiagnosis.
The technical scheme of the invention is that the frozen slice immunohistochemical dyeing machine and the method for immunohistochemically dyeing the frozen slice by the dyeing machine are characterized in that the frozen slice immunohistochemical dyeing machine comprises a body, a dyeing square bin, a reagent bottle assembly, a slide glass assembly, a sample injection needle assembly, a mechanical arm, a heating assembly, a liquid path system, a storage box, a refrigerating box and a desktop host, wherein the dyeing square bin, the reagent bottle assembly, the slide glass assembly, the sample injection needle assembly, the mechanical arm, the heating assembly, the liquid path system, the storage; dyeing side storehouse is located the upper portion of body, converges the pond and is located the middle part, and bin and fridge are located the lower part, and desk-top host computer is located the upper left side of body.
A frozen section immunohistochemical dyeing machine is provided with N (N ≧ 2) dyeing square bins which can be physically isolated or functionally partitioned, and each dyeing square bin comprises an independent reagent bottle assembly, a slide glass assembly, a loading needle assembly, a mechanical arm and a heating assembly. The application of sample arm is located the upper portion in dyeing square storehouse, and reagent bottle subassembly and application of sample needle subassembly are located the middle part, and the lower part is equipped with heating element, slide glass subassembly and mode conversion arm, and slide glass subassembly direct placement is on heating element, and every dyeing square storehouse all has the function of independently accomplishing immunohistochemical dyeing. The reagent bottle assembly comprises a reagent bottle rack and reagent bottles, wherein the reagent bottle rack is of a cuboid box structure and is used for fixing one reagent bottle group, a round hole is formed in the position, corresponding to the bottom of each reagent bottle, of the bottom of each reagent bottle, the position is used for pressing each reagent bottle to extrude a detection reagent through a mechanical arm, a fixing inserting plate is arranged at the top of each reagent bottle and is used for fixing the reagent bottles, liquid level detection holes are formed in the two sides of each reagent bottle rack, and a handheld position is arranged on the side face of each reagent bottle and is used for assembling; the reagent bottle mouth is provided with a bottle mouth quantitative liquid distributor, and a sealing cap is arranged on the dropping hole; the slide module comprises a slide bracket, a fixing plate, a current limiter and an electromagnetic switch, and is used for supporting and fixing a slide and limiting a reagent after sample loading in a sample area to be tested, the electromagnetic switch is controlled by current and is adaptive to the requirement of mode conversion, and the slide module has a sample loading reaction mode and a washing mode and has the functions of horizontal/inclined position conversion and mixed liquid shaking; both the reagent bottle assembly and the slide are adapted to all staining square bins.
The sample adding needle assembly comprises a sample adding needle, a sample adding needle bracket, a color developing agent bottle box and the like, a plurality of sample adding needles are arranged on a station corresponding to a glass slide (sample to be detected), and detection reagents are independently dripped into each slice respectively.
Placed large-size reagent bottle and waste liquid bucket in the storage box for store stationary liquid, buffer solution and collect the waste liquid, be equipped with the liquid level detection ware on the stock solution bottle, ensure that reagent can not be too few and the waste liquid can not too much spill over.
The refrigerated container is used for storing the reagent bottle assembly.
The liquid path system comprises a sample adding liquid path and a liquid discharging liquid path, wherein the inlet end of the sample adding liquid path is connected with a fixed liquid reagent bottle, a buffer solution reagent bottle and a color developing solution reagent bottle, and the outlet end of the sample adding liquid path is connected with a sample adding needle in the dyeing square bin in a branching manner after passing through a diaphragm liquid pump and a pressure reducing valve; the inlet end of the liquid drainage path is connected with the confluence tank, the outlet end of the liquid drainage path is connected with the waste liquid bottle, and waste liquid is collected into the waste liquid bottle by means of natural flow.
The mechanical arm assembly comprises a sample adding mechanical arm and a mode conversion mechanical arm, the sample adding mechanical arm and the mode conversion mechanical arm are respectively arranged at the top and the lower part of the dyeing shelter and are composed of a transmission mechanism and a driving motor, the sample adding mechanical arm is positioned above the reagent bottle assembly and acts on a reagent bottle in the reagent bottle assembly, a reagent in the reagent bottle is dripped to a sample to be detected, the mode conversion mechanical arm is positioned below the slide glass assembly and is close to the position of a slide glass handheld position and acts on the slide glass assembly, and the position of the slide glass is changed to meet the requirements of sample adding reaction and washing of two.
The desktop host controls the mechanical arm, the temperature control component, the liquid path component and the like through a digital/analog module; the desktop host computer accurately controls the experimental process of the dyeing equipment through the immunohistochemical experimental process module.
The method for realizing rapid immunohistochemical staining of the frozen section by depending on the frozen section immunohistochemical staining machine comprises the following steps: combining a sample from organ (tissue) source and prepared frozen sections into a group, and simultaneously carrying out sample adding and staining in a staining square bin; preferably, the catalytic enzyme is marked on the first antibody, and the 'one-step method' is adopted for dyeing, so that the aim of quickly carrying out immunohistochemical dyeing on successively delivered specimens is fulfilled, and the clinical requirement of carrying out pathological diagnosis on a frozen section in a short time is met.
The invention has the beneficial effects that: (1) by the frozen section immunohistochemical staining machine and the optimized immunohistochemical staining method, the frozen section immunohistochemical staining can be completed within 20 minutes, and the technical specification requirements of a clinician for making a frozen section pathological diagnosis within 30 minutes are met.
(2) A plurality of (N ≧ 2) independent immunohistochemical staining square bins are arranged on one freezing immunohistochemical staining machine, and the requirements of one-by-one inspection of clinical frozen detection specimens and rapid clinical pathological diagnosis can be met.
(3) Frozen sections of an organ (tissue) are combined into a group, and reagents are added into a staining square bin respectively and simultaneously for detection, so that the experimental flow time is greatly shortened.
(4) A group of antibody reagents commonly used (preset) for organs (tissues) are arranged on a reagent bottle assembly, so that the reagent bottles can be conveniently and rapidly arranged in a dyeing square bin for detection and can be detached and stored after being used.
(5) A frozen section of an organ (tissue) is placed on a slide glass assembly to form a group, and the frozen section can be simply placed on a position, matched with an antibody in a reagent bottle assembly, in a staining square bin for detection.
(6) The reagent bottle component and the slide glass component are adapted to all the dyeing square bins and are more than the dyeing square bins in number, so that the requirement of combined change of a plurality of same type of organ (tissue) samples or different types of organ (tissue) samples for delivery inspection in a short time can be met.
(7) The slide glass component is provided with a sample adding reaction mode and a washing mode, and the position and the angle of the slide glass are changed when the sample is reacted and washed, so that the detection reaction speed is increased, and the washing efficiency is improved.
(8) A plurality of sample adding needles are arranged on a dyeing station corresponding to each glass slide (detection sample), and a reagent is fixedly dripped into each sample adding needle, so that the reagent can be dripped into all the slices quickly and simultaneously, and the sample adding time is shortened.
(9) Based on the immunohistochemical staining method of the frozen section immunohistochemical staining machine, catalytic enzyme is preferably marked on the first antibody, and staining is performed by adopting a one-step method, so that the immunohistochemical staining time is shortened.
Drawings
FIG. 1 is a schematic view of a frozen section immunohistochemical staining machine according to the present invention.
FIG. 2 is a schematic view of the dyeing square bin structure of the present invention.
FIG. 3 is a schematic view of the structure of the reagent bottle assembly of the present invention.
Fig. 4 is a schematic structural diagram of the slide assembly of the present invention.
Fig. 5 is a schematic view of the structure of the robot arm of the present invention.
FIG. 6 is a schematic structural view of a sample injection needle assembly of the present invention.
Fig. 7 is a schematic connection diagram of the fluid path system of the present invention.
FIG. 8 is a flow chart of the dyeing method of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, it should be noted that the terms "upper", "lower", "left", "right", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, which are merely for convenience of description and simplicity of description, but do not mean or imply that the designated devices or elements must have a specific orientation, and furthermore, the terms "mounted" and "connected" in the description of the present invention should be construed broadly.
The technical scheme of the invention is further described in detail by combining the drawings and the embodiments in the specification.
As shown in figure 1, the frozen slice immunohistochemical dyeing machine comprises a body 1, a dyeing square bin 2, a reagent bottle assembly 3, a slide glass assembly 4, a mechanical arm 5, a heating assembly 6, a host machine 7, a storage box 8, a refrigerating box 9 and a confluence pool 10, wherein the dyeing square bin 2, the heating assembly, the reagent bottle assembly, the slide glass assembly, the mechanical arm 5, the heating assembly 6, the host machine 7, the storage box 8, the refrigerating box 9 and the confluence pool 10 are arranged in the body 1, the body 1 is of a box structure, the dyeing square bin 2 is located at the upper portion of the body 1, the confluence pool 10. A frozen slice immunohistochemical staining machine is provided with N (N ≧ 2) staining square bins 2, which can be physically isolated or functionally partitioned, and is illustrated as a frozen slice immunohistochemical staining machine having 5 staining square bins 2.
As shown in fig. 2, each staining square bin comprises a single reagent bottle component 3, a slide glass component 4, a mechanical arm 5, a sample injection needle component 21 and a heating component 6. Arm 5 is located the upper portion of dyeing square bin 2, and reagent bottle subassembly 3 and application of sample needle subassembly 21 are located the middle part, and the lower part is heating element 6 and slide glass subassembly 4, and slide glass subassembly 4 direct mount is on heating element 6, and every dyeing square bin all has the function of independently accomplishing immunohistochemical dyeing.
As shown in fig. 3, the reagent bottle assembly 3 includes a reagent bottle 31, a fixing insertion plate 32 and a reagent bottle holder 33, the reagent bottle holder 33 is a rectangular box structure for fixing a reagent bottle group, a hand-held portion 331 is disposed on a side surface of the reagent bottle holder, a round hole 332 is disposed on a portion of the top corresponding to a reagent bottle opening, a round hole 333 is disposed on a portion of the bottom corresponding to a reagent bottle bottom, the reagent bottle is pressed by the mechanical arm 5 to extrude a detection reagent, and a bottle opening quantitative dispenser 311 is disposed at a mouth of the reagent bottle 31.
As shown in fig. 4, the slide assembly 4 includes a fixed platen 41, a slide holder 42, a current limiter 411 and an electromagnetic switch 412, and is used for supporting and fixing a slide 43, and limiting the reagent after sample loading on the sample area to be tested, the electromagnetic switch 412 is adapted to the requirement of mode conversion, the slide assembly 4 has a sample loading reaction mode and a washing mode, and has the functions of horizontal/inclined position conversion and shaking mixed liquid, and one side of the slide holder 42 is provided with a handheld position 421 and a fixed buckle 422.
As shown in fig. 5, the robot 5 includes a sample application robot 51, a mode switching robot 52, a moving stage 53, a guide rail 54, a driving motor 55, a timing pulley 56, a driven wheel 57, and a timing belt 58, wherein the sample application robot 51 acts on the reagent bottle 31, and the mode switching robot 52 acts on the slide module 4.
As shown in fig. 6, the sample application needle assembly 21 includes a liquid channel system holder 211, a developer liquid pump 212, a sample application needle holder 213, a sample application needle 214, a developer bottle box 215, and a developer bottle 216, and a plurality of sample application needles 214 are provided at stations corresponding to one slide glass 43 (sample to be measured), and reagents are independently dropped to each section.
As shown in fig. 7, the liquid path system is divided into a sample feeding path and a liquid discharging path, the inlet end of the sample feeding path is connected to a stationary liquid bottle 81, a buffer liquid bottle 82 and a color developing liquid bottle 216, respectively, and the outlet end is branched and then connected to a dyeing side, a sample feeding needle 214 in 2, which is connected to a diaphragm liquid pump 01 and a pressure reducing valve 02; the inlet end of the liquid drainage path is connected with the confluence tank 10, the outlet end is connected with the waste liquid bottle 83, and the waste liquid is collected into the waste liquid bottle 83 by a natural flowing mode.
As shown in fig. 8, the flow chart of the dyeing method includes: the reagent bottle assembly 3 is installed S1, the slide glass assembly 4 is installed S2, the reagent bottle assembly 3 and the slide glass assembly 4 are inserted into the staining square bin 2S 3, the specimen is fixed S4, the S5 is washed, the antibody is dripped S6, the S7 is washed, the S8 is developed, the S9 is washed, and the slide glass assembly 4 is taken out S10.
The technical solution of the present invention will be further described by the following detailed description of the working process of the frozen section immunohistochemical staining machine. The working process of the fully automatic immunohistochemical system comprises the following steps.
1. Reagent bottle subassembly 3 installation: the reagent bottle 31 for detecting 1 organ (tissue) marker molecule is put into a reagent bottle rack 33 to be assembled into a reagent bottle assembly 3, and the reagent bottle assembly 3 is stored in a 4-degree refrigerator. When in use, the bottle mouth is downwards inserted into the station in the dyeing square bin 2.
2. Slide subassembly 4 installation: 1 organ (tissue) specimen for clinical examination is prepared into a frozen section, and is placed on a slide holder 42, a fixed pressing plate 41 is covered, and the slide assembly 4 is assembled and inserted into a station in the staining square bin 2. The marker molecules detected by the specimen on the slide 43 are identical to the types of antibodies in the reagent bottle assembly 3.
3. Tissue fixation: the experiment begins, and the stationary liquid 81 suction in the liquid way system will be stored bin 8 and simultaneously the dropwise add to slide glass 43 of slide glass subassembly 4 on, fix the sample that detects, slide glass subassembly 4 is in the detection position this moment, keeps horizontal position.
4. Washing: the mode-switching mechanical arm 52 switches the slide assembly 4 to the wash position, the electromagnetic switch 412 is turned on, the slide 43 is tilted by about 30 degrees, the fixative solution flows out, and then the buffer solution is dripped onto the slide 43 by the liquid path system to wash the specimen.
5. Antigen-antibody reaction: after the washing is completed, the mode conversion mechanical arm 52 converts the slide glass assembly 4 to the detection position, at this time, the slide glass is in the horizontal position, the electromagnetic switch 412 is turned off, the sample adding mechanical arm 51 moves downward, contacts with the bottom of the reagent bottle 31 and pushes the reagent bottle 31 downward, and the antibody in the reagent bottle 31 is added to the sample of the slide glass 43 by dropping, so that the antigen-antibody reaction is performed.
6. Washing: after the reaction is completed, the slide module 4 is switched to the detection position by the mode switching mechanical arm 52, the slide 43 is tilted by about 30 degrees, the electromagnetic switch 412 is turned on, the antibody reagent solution flows out, and then the buffer solution is dripped onto the slide 43 by the liquid path system, and the specimen is washed.
7. Color development: after the washing is completed, the mode switching mechanical arm 52 switches the slide module 4 to the detection position, the slide 43 is in the horizontal position, and the liquid path system drops the developer in the developer bottle 216 onto the sample of the slide 43 for developing the color.
8. Washing: the mode switching mechanical arm 52 switches the slide assembly 4 to the washing position, the electromagnetic switch 412 is turned on, the slide 43 is tilted by about 30 degrees, the developer flows out, and then the liquid path system drips the buffer liquid onto the slide 43 to wash the specimen.
The whole process is accurately controlled by the host 7.
It should be noted that the above description of the specific embodiments of the present invention is only for the purpose of illustrating the technical lines and features of the present invention, and the purpose of the present invention is to enable those skilled in the art to understand the content of the present invention and to implement the present invention, but the present invention is not limited to the above specific embodiments. It is intended that all such changes and modifications as fall within the scope of the appended claims be embraced therein.
Claims (10)
1. A frozen section immunohistochemical staining machine and a method are characterized by comprising the frozen section immunohistochemical staining machine and a method for immunohistochemical staining of frozen sections based on the staining machine;
the frozen slice immunohistochemical dyeing machine is characterized by comprising a body, a dyeing square bin, a reagent bottle assembly, a slide glass assembly, a sample feeding needle assembly, a mechanical arm, a temperature control assembly, a confluence tank, a liquid path assembly, a storage tank, a refrigerating box and a desktop host which are arranged on the body, wherein the dyeing square bin is positioned at the upper part of the body, the confluence tank is positioned at the middle part, the storage tank and the refrigerating box are positioned at the lower part, and the host is positioned at the upper left part;
the dyeing square bin is characterized by comprising a reagent bottle component, a slide glass component, a sample injection needle component, a mechanical arm and a heating component;
the reagent bottle assembly is characterized by comprising a reagent bottle rack and reagent bottles, wherein the reagent bottle rack is provided with a handheld position, and the reagent bottle opening is provided with a bottle opening quantitative liquid distributor;
the slide glass component is characterized by comprising a slide glass bracket, a fixed pressing plate, a current limiter and a magnetic switch, wherein the slide glass bracket is used for supporting and fixing a slide glass, limiting a reagent after sample loading in a sample area to be tested, and has a mode conversion function;
the sample injection needle assembly is characterized in that each staining station of each slide is provided with an independent sample injection needle;
the method is characterized in that the frozen section is quickly subjected to immunohistochemical staining by means of the frozen section immunohistochemical staining machine.
2. A frozen section immunohistochemical staining machine and method according to claim 1 wherein there are N (N ≧ 2) staining chambers on a single apparatus, either physically segregated or functionally segregated.
3. The immunohistochemical staining machine and method of frozen sections of claim 1 wherein the bottom of the staining square chamber is the heating element and slide element, above which are the reagent vial element and the loading needle element, the loading robot is located at the top, and the mode switching robot is located below the holding position of the slide.
4. An immunohistochemical staining machine and method of frozen sections according to claim 1 wherein each staining square compartment has the function of independently performing immunohistochemical staining.
5. The machine and method for immunohistochemical staining of frozen sections according to claim 1, wherein the reagent bottle assembly is a reagent bottle assembly comprising a plurality of reagent bottles, and wherein the reagent bottles are simultaneously dropped onto different samples to be tested.
6. A staining machine and method for immunohistochemistry of frozen sections as claimed in claim 1, wherein the slide assembly is a slide assembly formed by assembling 1 kind of organ (tissue) sections, and according to the marker molecule to be detected, the matched reagent bottle assembly is installed and the sample is added for detection.
7. The immunohistochemical staining machine and method for frozen sections according to claim 1, wherein the slide assembly is provided with a current limiter and an electromagnetic switch, which can realize the conversion between the sample loading reaction mode and the washing mode, and has the horizontal/inclined conversion function to improve the reaction and washing efficiency.
8. The immunohistochemical staining machine and method for frozen sections according to claim 1, wherein each section station is equipped with a set of sample application needles, each needle being fixed and dropped with a detection reagent to perform rapid simultaneous sample application and staining of a set of sections.
9. An immunohistochemical staining machine and method of frozen sections as defined in claim 1 wherein reagent bottle assembly and slides are adapted to all staining square bins for easy and quick installation and removal.
10. A frozen section immunohistochemical staining machine and method according to claim 1, wherein the first antibody is directly labeled with catalytic enzyme and staining is performed by "one-shot method".
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010764103.0A CN111693354A (en) | 2020-08-01 | 2020-08-01 | Frozen slice immunohistochemical staining machine and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010764103.0A CN111693354A (en) | 2020-08-01 | 2020-08-01 | Frozen slice immunohistochemical staining machine and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111693354A true CN111693354A (en) | 2020-09-22 |
Family
ID=72486523
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010764103.0A Pending CN111693354A (en) | 2020-08-01 | 2020-08-01 | Frozen slice immunohistochemical staining machine and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111693354A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683633A (en) * | 2021-01-19 | 2021-04-20 | 百盛(广州)生物制品有限公司 | Full-automatic immunohistochemical dyeing machine |
| CN114279798A (en) * | 2021-04-25 | 2022-04-05 | 福州安林生物科技有限公司 | Full-automatic immunohistochemical staining instrument and staining method thereof |
| CN114323833A (en) * | 2021-09-30 | 2022-04-12 | 北京海格莱生物科技有限公司 | Immunohistochemical specimen processing device and method adopting paraffin section mode |
| CN117538128A (en) * | 2024-01-10 | 2024-02-09 | 苏州大学附属第二医院 | Pathology department's sample section dyeing equipment |
-
2020
- 2020-08-01 CN CN202010764103.0A patent/CN111693354A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683633A (en) * | 2021-01-19 | 2021-04-20 | 百盛(广州)生物制品有限公司 | Full-automatic immunohistochemical dyeing machine |
| CN114279798A (en) * | 2021-04-25 | 2022-04-05 | 福州安林生物科技有限公司 | Full-automatic immunohistochemical staining instrument and staining method thereof |
| CN114279798B (en) * | 2021-04-25 | 2024-04-02 | 福州安林生物科技有限公司 | Full-automatic immunohistochemical staining instrument and staining method thereof |
| CN114323833A (en) * | 2021-09-30 | 2022-04-12 | 北京海格莱生物科技有限公司 | Immunohistochemical specimen processing device and method adopting paraffin section mode |
| CN117538128A (en) * | 2024-01-10 | 2024-02-09 | 苏州大学附属第二医院 | Pathology department's sample section dyeing equipment |
| CN117538128B (en) * | 2024-01-10 | 2024-03-15 | 苏州大学附属第二医院 | Pathology department's sample section dyeing equipment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111693354A (en) | Frozen slice immunohistochemical staining machine and method | |
| CN110007099B (en) | Automatic analyzer and sample analyzing method | |
| EP3572815B1 (en) | Automatic analysis device and sample analysis method | |
| US4785677A (en) | Pipetting device having an automatic mechanism for replacing nozzle tips | |
| US6096271A (en) | Random access slide stainer with liquid waste segregation | |
| US6825041B2 (en) | Method and system for automated immunochemistry analysis | |
| EP3671218A1 (en) | Blood analyzer and control method therefor | |
| MXPA06000249A (en) | Automated multi-detector analyzer. | |
| CN116840499A (en) | Automatic analysis device and sample analysis method thereof | |
| JP2013033036A (en) | Instrument and process for automatic processing of liquid sample | |
| JP2007537448A (en) | Calibration solution system for use in automated clinical analyzers | |
| CN216747755U (en) | Full-automatic fluorescent quantitative analyzer | |
| US20220026321A1 (en) | Specimen processing systems, pipette assemblies and methods for preparing reagents | |
| CN113804906A (en) | Full-automatic fluorescent quantitative analyzer | |
| CN114019178A (en) | Full-automatic immune biochemical integrated analyzer and use method thereof | |
| CN113049800A (en) | Immunoassay analyzer, detection method thereof and computer readable storage medium | |
| CN214066682U (en) | Frozen section immunohistochemical dyeing machine | |
| US5271899A (en) | Chemistry analyzer | |
| EP3444033B1 (en) | Cartridge for chemical or biological assays | |
| CN112834766B (en) | Immunoblotter and control method | |
| CN110244070B (en) | Specific protein analyzer and test method thereof | |
| CN111855995A (en) | Incubation device | |
| JP2022072939A (en) | Automatic analyzer | |
| CN212845408U (en) | Incubation device | |
| JPH06308132A (en) | Automatic analyzing apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |