CN112683633A - Full-automatic immunohistochemical dyeing machine - Google Patents
Full-automatic immunohistochemical dyeing machine Download PDFInfo
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- CN112683633A CN112683633A CN202110070575.0A CN202110070575A CN112683633A CN 112683633 A CN112683633 A CN 112683633A CN 202110070575 A CN202110070575 A CN 202110070575A CN 112683633 A CN112683633 A CN 112683633A
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- 238000004043 dyeing Methods 0.000 title claims abstract description 34
- 230000002055 immunohistochemical effect Effects 0.000 title claims abstract description 17
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 59
- 238000005070 sampling Methods 0.000 claims abstract description 57
- 239000000427 antigen Substances 0.000 claims abstract description 31
- 102000036639 antigens Human genes 0.000 claims abstract description 31
- 108091007433 antigens Proteins 0.000 claims abstract description 31
- 230000005540 biological transmission Effects 0.000 claims abstract description 23
- 238000011532 immunohistochemical staining Methods 0.000 claims description 22
- 239000011521 glass Substances 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 14
- 238000000429 assembly Methods 0.000 claims description 8
- 230000000712 assembly Effects 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 4
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- 239000012744 reinforcing agent Substances 0.000 description 2
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- QNRATNLHPGXHMA-XZHTYLCXSA-N (r)-(6-ethoxyquinolin-4-yl)-[(2s,4s,5r)-5-ethyl-1-azabicyclo[2.2.2]octan-2-yl]methanol;hydrochloride Chemical compound Cl.C([C@H]([C@H](C1)CC)C2)CN1[C@@H]2[C@H](O)C1=CC=NC2=CC=C(OCC)C=C21 QNRATNLHPGXHMA-XZHTYLCXSA-N 0.000 description 1
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Abstract
The invention discloses a full-automatic immunohistochemical dyeing machine, which comprises a box body, a transmission system and a sampling system, wherein the box body is enclosed to form a cavity, the top of the box body is provided with a top cover which can be opened and closed, the bottom of the cavity is provided with a carrying platform, a reagent rack, a secondary antibody system and an antigen restoration instrument are arranged on the carrying platform, the transmission system is positioned at the upper ends of the reagent rack, the secondary antibody system and the antigen restoration instrument, the sampling system is arranged on the transmission system, the transmission system drives the sampling system to move, the reagent rack, the secondary antibody system and the antigen restoration instrument are sequentially arranged along the moving direction of the sampling system, the sampling system comprises sampling needles, the number of the sampling needles is 2-16, each sampling needle, and each sampling needle can independently move along the height direction of the box body, and the full-automatic immunohistochemical dyeing machine replaces manual immunohistochemical dyeing, standardizes the dyeing process and improves the dyeing efficiency.
Description
Technical Field
The invention relates to the technical field of medical instruments and scientific instruments, in particular to a full-automatic immunohistochemical dyeing machine.
Background
With the annual increase of the incidence of tumors, the complex annual growth rate of immunohistochemical staining experiments for tumor diagnosis related is 15%, resulting in an increasing workload and working intensity of immunohistochemical experiments. At present, the full-automatic immunohistochemical dyeing machines on the market are mainly imported brands such as roche, come card, and danke, the quantity of dyeing pieces in each round is 20-48, the dyeing pieces are mainly limited by a sample adding technology of a single channel and a glass slide placing technology, and the dyeing piece quantity of a single instrument cannot be rapidly increased.
Disclosure of Invention
The invention aims to solve at least one technical problem in the prior art and provides a full-automatic immunohistochemical dyeing machine with high integration degree and multi-channel sample adding.
According to the embodiment of the first aspect of the invention, a full-automatic immunohistochemical dyeing machine is provided, which comprises a box body, a transmission system and a sampling system, the box body is enclosed to form a cavity, the top of the box body is provided with a top cover which can be opened and closed, the bottom of the cavity is provided with a carrying platform, the carrying platform is provided with a reagent rack, a secondary antibody system and an antigen retrieval instrument, the transmission system is positioned at the upper ends of the reagent rack, the secondary antibody system and the antigen retrieval instrument, the sampling system is arranged on the transmission system, the transmission system drives the sampling system to move, the reagent rack, the secondary antibody system and the antigen retrieval instrument are sequentially arranged along the moving direction of the sampling system, the sampling system comprises sampling needles, the number of the sampling needles is 2-16, each sampling needle is provided with a metering valve, and each sampling needle can independently move along the height direction of the box body.
Has the advantages that: this full-automatic immunohistochemical dyeing machine, the power distribution box comprises a box body, transmission system and sampling system, the box encloses to close and is formed with the cavity, the top of box is provided with the top cap that can open and shut, the cavity bottom is provided with the microscope carrier, be provided with the reagent frame on the microscope carrier, two antibody system and antigen restoration appearance, transmission system is located the reagent frame, two antibody system and antigen restoration appearance upper end, sampling system sets up on transmission system, transmission system drive sampling system removes, the reagent frame, two antibody system and antigen restoration appearance set gradually along the sampling system moving direction, sampling system includes two piece at least sampling needles, each sampling needle all is provided with the metering valve, and the direction of height independent motion of box can be followed to each sampling needle, this full-automatic immunohistochemical dyeing machine replaces manual immunohistochemical dyeing, the standard dyeing flow, the efficiency of dyeing is improved.
The full-automatic immunohistochemical staining machine according to an embodiment of the first aspect of the present invention further comprises an air exhaust system disposed on the top cover.
According to the full-automatic immunohistochemical staining machine provided by the embodiment of the first aspect of the invention, the transmission system comprises a guide rail, a pipetting arm sliding along the guide rail, and a driving mechanism, two ends of the guide rail are fixed on the box body, the driving mechanism drives the pipetting arm to slide along the guide rail, and the sampling system is arranged on the pipetting arm.
According to the full-automatic immunohistochemical dyeing machine provided by the embodiment of the first aspect of the invention, the box body comprises two side frames and two main frames, one main frame is provided with a door body, two ends of the guide rail are respectively fixed on the two side frames of the box body, and a plurality of grid holes are respectively formed in the two side frames.
According to the full-automatic immunohistochemical staining machine of the embodiment of the first aspect of the invention, the secondary antibody system comprises a plurality of reagent box modules and a needle washing groove module.
According to the full-automatic immunohistochemical staining machine provided by the embodiment of the first aspect of the invention, the reagent rack comprises a first rack plate and a second rack plate which are oppositely arranged up and down, and a plurality of reagent holes for installing reagent pipes are oppositely arranged on the first rack plate and the second rack plate.
According to the full-automatic immunohistochemical staining machine provided by the embodiment of the first aspect of the invention, the antigen retrieval instrument comprises a water bath box body, a heating groove is formed in the inner side of the water bath box body, a plurality of placing tables are arranged in the heating groove, and multiple slide glass placing frames are placed on the placing tables.
According to the full-automatic immunohistochemical staining machine provided by the embodiment of the first aspect of the invention, the multi-linked slide rack comprises a main board, a plurality of slide fixing grooves are arranged on the main board at equal intervals, and slide assemblies are inserted into the slide fixing grooves.
According to the full-automatic immunohistochemical staining machine provided by the embodiment of the first aspect of the invention, the glass slide assembly comprises a fixing frame and a glass slide, the fixing frame comprises a clamping plate plane, the left side and the right side of the clamping plate plane are symmetrically provided with clamping plate side walls, the inner sides of the clamping plate side walls are respectively provided with a clamping groove, the front end of the clamping plate plane is connected with a sample adding port, and the sample adding port comprises a funnel plate surface, the inner side of the funnel plate surface is connected with the clamping plate plane, and the outer side of.
According to the full-automatic immunohistochemical dyeing machine, the top cover is movably connected with one side edge of the top surface of the box body in a switching mode, a hydraulic rod is arranged in the box body, the movable end of the hydraulic rod is connected with the top cover, and the hydraulic rod pushes the top cover to be opened and closed.
Drawings
The invention is further described below with reference to the accompanying drawings and examples;
FIG. 1 is a schematic view of a fully automatic immunohistochemical staining machine according to an embodiment of the present invention;
FIG. 2 is a schematic view of an antigen retrieval instrument according to an embodiment of the present invention;
FIG. 3 is a schematic view of a reagent rack according to an embodiment of the present invention;
FIG. 4 is a schematic view of a secondary antibody system according to an embodiment of the present invention;
FIG. 5 is a schematic view of a slide assembly according to an embodiment of the present invention;
fig. 6 is a schematic view of a multiple slide rack according to an embodiment of the invention.
Detailed Description
Reference will now be made in detail to the present preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout.
In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.
Referring to fig. 1 to 6, a full-automatic immunohistochemical staining machine includes a box 100, a transmission system 500 and a sampling system 600, wherein the box 100 encloses to form a cavity 110, a top cover 120 capable of opening and closing is disposed on the top of the box 100, a carrying platform 130 is disposed on the bottom of the cavity 110, a reagent rack 200, a secondary antibody system 300 and an antigen retrieval instrument 400 are disposed on the carrying platform 130, the transmission system 500 is disposed on the reagent rack 200, the second antibody system 300 and the antigen retrieval instrument 400 are arranged at the upper end, the sampling system 600 is arranged on the transmission system 500, the transmission system 500 drives the sampling system 600 to move, the reagent rack 200, the second antibody system 300 and the antigen retrieval instrument 400 are sequentially arranged along the moving direction of the sampling system 600, the sampling system 600 comprises sampling needles 610, the number of the sampling needles 610 is 2-16, each sampling needle 610 is provided with a metering valve, and each sampling needle 610 can independently move along the height direction of the box body 100.
In order to ensure the cleanliness of the interior of the dyeing machine, avoid reagent contamination and reduce reagent utilization, a top cover 120 is designed to be openable. The top cover 120 is movably connected with one side edge of the top surface of the box body 100 in a rotating mode, a hydraulic rod 160 is arranged in the box body 100, the movable end of the hydraulic rod 160 is connected with the top cover 120, and the hydraulic rod 160 pushes the top cover 120 to be opened and closed. Hydraulic rod 160 is located in cavity 110, when the dyeing machine is running, hydraulic rod 160 contracts, top cover 120 is automatically closed, and after the dyeing machine is running, hydraulic rod 160 pushes the top cover outwards to automatically open. In some of these embodiments, the fully automated immunohistochemical staining machine includes a tank 100, a drive system 500, a sampling system 600, and an exhaust system 700. Wherein, the top of the box 100 is provided with a top cover 120 which can be opened and closed, and the exhaust system 700 is arranged on the top cover 120. Exhaust system 700 is convenient for the discharge of immunohistochemical dyeing in-process waste gas, and the top cap 120 of whole dyeing machine is streamlined design, accords with the size of sampling system 600 and transmission system 500 completely, guarantees to move the liquid smoothly.
The transmission system 500 includes a guide rail 510, a pipetting arm 520 sliding along the guide rail 510, and a driving mechanism 530, two ends of the guide rail 510 are fixed on the box 100, the driving mechanism 530 drives the pipetting arm 520 to slide along the guide rail 510, and the sampling system 600 is disposed on the pipetting arm 520.
Referring to fig. 1, the cabinet 100 includes two side frames 140 and two main frames 150, one main frame 150 is provided with a door body, two ends of the guide rail 510 are respectively fixed on the two side frames 140 of the cabinet 100, and a plurality of grid holes are respectively formed on the two side frames 140. It is understood that the reagent rack 200, the second antibody system 300, and the antigen retrieval instrument 400 are inconvenient to take out and place because a slide rail is provided above the reagent rack 200, the second antibody system 300, and the antigen retrieval instrument 400. With the slidable setting of door body, can conveniently open box 100, get and put reagent frame 200, two antibody system 300 and antigen retrieval appearance 400 that are located cavity 110, make things convenient for the experimentation. The side frame 140 is provided with a plurality of rhombic grid holes, so that air circulation and ventilation are facilitated during the operation of the liquid-transferring safety and air-exhaust system.
Referring to fig. 4, in particular, the secondary antibody system 300 includes several reagent cartridge modules and one needle wash tank module. In the present embodiment, the secondary antibody system 300 is composed of four modules. The first module consists of 6 15ml reagent kits, the second module consists of four 30ml reagent kits, the third module consists of 2 60ml reagent kits, and the fourth module consists of a needle washing groove and a 60ml reagent kit. Can be freely combined, flexible and various according to the actual experiment requirements. The reagent box is mainly provided with the following components: dewaxing solution, absolute ethyl alcohol, purified water, cleaning solution, antigen repairing solution, HRP reagent, reinforcing agent, DAB, hematoxylin, pickling solution and other reagents.
Referring to fig. 3, the reagent rack 200 includes a first rack plate 210 and a second rack plate 220 which are arranged up and down, and a plurality of reagent holes for installing reagent tubes are arranged on the first rack plate 210 and the second rack plate 220. In this embodiment, the reagent rack 200 is provided with 6 columns of reagent wells in 16 rows for a total of 96 reagent tube positions. The reagent rack 200 is arranged into an upper layer and a lower layer, so that the reagent tubes can be kept vertical better to facilitate sampling. The aperture of each reagent hole is 17 mm. The sampling system 600 can automatically position the reagent position, absorb the sample according to the set sampling amount (10-1000 μ l), and add the sample to the sample addition port of the antigen retrieval instrument 400 according to the set program, and the whole process is fully-automatic and self-completed.
Referring to fig. 2, 5 and 6, the antigen retrieval instrument 400 includes a water bath box body, a heating groove is provided on the inner side of the water bath box body, a plurality of placing tables are provided in the heating groove, and a multi-slide placing rack 410 is placed on each placing table. The groove is hatched as to the heating tank, antigen restoration appearance still includes temperature probe, drainage system, communication system, electrical power generating system etc. are constituteed, the handle that accords with human engineering mechanics has been designed respectively about antigen restoration appearance, be convenient for carry, drainage system divide into clear solution outlet and waste liquid outlet, by procedure automatic control, the separately collection and the emission of the waste liquid of being convenient for, antigen restoration appearance bottom sets up the level of antiskid callus on the sole adjusting instrument and prevents to remove, cooling system adopts air-cooled mode, according to different experimental conditions, realize controllable temperature regulation, whole shell adopts high temperature resistant environmental protection material, do not produce deformation, the release of no harmful substance, realize safety in production, environmental protection production. The multiple slide rack 410 includes a main plate 411, a plurality of slide fixing grooves 412 are equidistantly arranged on the main plate 411, and slide assemblies 413 are inserted into the slide fixing grooves 412. Slide glass subassembly 413 includes fixed frame and slide glass, and fixed frame includes the cardboard plane, and cardboard plane left and right sides symmetry is provided with the cardboard lateral wall, and cardboard lateral wall inboard all is provided with the draw-in groove, and cardboard plane front end is connected with the sample addition mouth, and the sample addition mouth includes the funnel face that inboard connection cardboard plane and outside slant down. After the glass slide assemblies 413 are installed, the glass slide assemblies are vertically placed on the multiple glass slide placing rack 410, the whole multiple glass slide placing rack 410 is placed on the antigen retrieval instrument, 12 or 20 glass slide assemblies can be placed on the antigen retrieval instrument, the clamping plates are vertically placed, the repairing liquid is just immersed into the repairing liquid below a position line of the repairing liquid, the repairing liquid moves upwards along the flowing direction of the liquid along with the rising of the temperature, the repairing liquid continuously flows upwards in the whole repairing process to ensure the repairing effect, the internal repairing temperature is higher than 100 ℃ along with the addition of the antigen repairing reinforcing agent and the sealing effect of the whole glass slide, and the dry plate phenomenon can not occur, and the high-temperature high-pressure steam repairing effect is greater than that of the traditional water bath repairing (less than or equal to 100 ℃) and electric baking plate repairing (less than 100 ℃) and the plate is easy to dry when the temperature is high, so that the dyeing effect of immunohistochemistry is enhanced, the background is reduced, and the use of reagents is reduced.
The sampling process of the full-automatic immunohistochemical staining machine is as follows: 1. the sampling needle 610 starts an automatic cleaning function, and the inner wall and the outer wall of the sampling needle 610 are cleaned in the needle washing groove; 2. inputting a reagent code of the antibody to be detected in a computer according to the reagent information of the reagent rack 200, and printing out two-dimensional codes, wherein one reagent corresponds to one two-dimensional code; 3. attaching reagent labels with two-dimensional codes to corresponding glass slide assemblies, reading the two-dimensional codes through a scanner, automatically identifying reagent information and positioning the positions of the glass slide assemblies; 4. the sampling needle 610 starts a liquid level detection function to confirm the presence of a reagent and automatically recognize the inhalation of non-liquid (such as air, solid, etc.); 5. the sampling needle 610 sucks the set reagent amount, and adds the reagent amount to the sample addition port according to the optimized moving track; 6. and repeating the steps 1-5 continuously until the set reagent is completely added.
The dyeing machine has three innovative technologies of multi-channel sample adding, 2 to 16 sampling needles 610 can be selected, circulating steam thermal restoration and vertical piece placement, the dyeing machine can complete simultaneous dyeing of at most 320 pieces, the completion time is controlled within three hours, and the dyeing machine is large in dyeing piece amount, rapid in sample adding, unique in thermal restoration and good in dyeing quality.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention.
Claims (10)
1. A full-automatic immunohistochemical staining machine, comprising:
the device comprises a box body, a cover, a reagent rack, a secondary antibody system and an antigen retrieval instrument, wherein the box body is enclosed to form a cavity, the top of the box body is provided with a top cover which can be opened and closed, the bottom of the cavity is provided with a carrying platform, and the carrying platform is provided with the reagent rack, the secondary antibody system and the antigen retrieval instrument;
the transmission system is positioned at the upper ends of the reagent rack, the secondary antibody system and the antigen retrieval instrument; and
the sampling system is arranged on the transmission system, the transmission system drives the sampling system to move, the reagent rack, the secondary antibody system and the antigen retrieval instrument are sequentially arranged along the moving direction of the sampling system, the sampling system comprises sampling needles, the number of the sampling needles is 2-16, each sampling needle is provided with a metering valve, and each sampling needle can independently move along the height direction of the box body.
2. The full-automatic immunohistochemical staining machine of claim 1, wherein: the full-automatic immunohistochemical dyeing machine further comprises an exhaust system, and the exhaust system is arranged on the top cover.
3. The full-automatic immunohistochemical staining machine of claim 1, wherein: the transmission system comprises a guide rail, a liquid transfer arm and a driving mechanism, wherein the guide rail slides, two ends of the guide rail are fixed on the box body, the driving mechanism drives the liquid transfer arm to slide along the guide rail, and the sampling system is arranged on the liquid transfer arm.
4. The full-automatic immunohistochemical staining machine of claim 3, wherein: the box body comprises two side frames and two main frames, one main frame is provided with a door body, two ends of the guide rail are respectively fixed on the two side frames of the box body, and a plurality of grid holes are formed in the two side frames.
5. The full-automatic immunohistochemical staining machine of claim 1, wherein: the secondary antibody system comprises a plurality of reagent box modules and a needle washing groove module.
6. The full-automatic immunohistochemical staining machine of claim 1, wherein: the reagent frame comprises a first support plate and a second support plate which are oppositely arranged from top to bottom, and a plurality of reagent holes for installing reagent tubes are oppositely arranged on the first support plate and the second support plate.
7. The full-automatic immunohistochemical staining machine of claim 1, wherein: the antigen retrieval instrument comprises a water bath box body, a heating groove is formed in the inner side of the water bath box body, a plurality of placing tables are arranged in the heating groove, and a multi-slide glass placing frame is placed on each placing table.
8. The full-automatic immunohistochemical staining machine of claim 7, wherein: the multi-connected glass slide placing rack comprises a main board, wherein a plurality of glass slide fixing grooves are arranged on the main board at equal intervals, and glass slide assemblies are inserted in the glass slide fixing grooves.
9. The full-automatic immunohistochemical staining machine of claim 8, wherein: the glass slide subassembly includes fixed frame and slide glass, fixed frame includes the cardboard plane, cardboard plane left and right sides symmetry is provided with the cardboard lateral wall, cardboard lateral wall inboard all is provided with the draw-in groove, cardboard plane front end is connected with the sample addition mouth, the sample addition mouth includes inboard funnel face of connecting cardboard plane and outside slope down.
10. The full-automatic immunohistochemical staining machine of claim 1, wherein: the top cover is movably connected with one side edge of the top surface of the box body in a switching mode, a hydraulic rod is arranged in the box body, the movable end of the hydraulic rod is connected with the top cover, and the hydraulic rod pushes the top cover to be opened and closed.
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| CN202110070575.0A CN112683633A (en) | 2021-01-19 | 2021-01-19 | Full-automatic immunohistochemical dyeing machine |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110070575.0A CN112683633A (en) | 2021-01-19 | 2021-01-19 | Full-automatic immunohistochemical dyeing machine |
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Application publication date: 20210420 |
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