CN208476787U - A kind of immunofluorescence dyeing kit - Google Patents
A kind of immunofluorescence dyeing kit Download PDFInfo
- Publication number
- CN208476787U CN208476787U CN201820920636.1U CN201820920636U CN208476787U CN 208476787 U CN208476787 U CN 208476787U CN 201820920636 U CN201820920636 U CN 201820920636U CN 208476787 U CN208476787 U CN 208476787U
- Authority
- CN
- China
- Prior art keywords
- reservoir
- liquid
- box body
- immunofluorescence dyeing
- cleaning solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of immunofluorescence dyeing kits, including box body, the surface of the box body is provided with the first reservoir, the inside of first reservoir is placed with permeable membrane liquid, the inside of second reservoir is placed with fluorescence secondary antibody, the inside of the third reservoir is placed with the first reparation liquid, the inside of 4th reservoir is placed with the second reparation liquid, the inside of 5th reservoir is placed with fixer, the inside of 6th reservoir is placed with cleaning solution, the inside of 7th reservoir is placed with confining liquid, the inside of 8th reservoir is placed with mounting liquid.The utility model repairs liquid, the second reparation liquid structure by setting fixer, cleaning solution, confining liquid, fluorescence secondary antibody, mounting liquid, box body, permeable membrane liquid, first, solves the problems, such as that influence factor is various and can not be applied to histofluorescence and dyes.
Description
Technical field
The utility model relates to biomedicine technical field, specially a kind of immunofluorescence dyeing kit.
Background technique
Immunofluorescence dyeing detection method is the important technology in life science and medical research, mainly utilizes antigen and resists
The specific binding of body, to be positioned in cell or tissue to specific albumen.This detection method utilizes fluorescence radiation
Principle, detection sensitivity grade is high, can be to specific protein other than subcellular level positions, can also be to two or more
Protein carries out common location detection in cell or tissue, plays an important role in life science and medical research.So
And immunofluorescence dyeing detection technique is cumbersome, at high cost, detection is influenced by many factors, therefore is significantly limited
It is applied.The utility model is specially a kind of immunofluorescence dyeing kit.
But there are deficiencies below for existing technology:
1, immunofluorescence dyeing technical operation is difficult, and detection sensitivity is affected by many factors, mainly has:
(1) type and concentration of fixer are different,
(2) concentration of permeable membrane liquid and time inaccuracy,
(3) type of fluorescence secondary antibody is different,
(4) mounting and the preservation for detecting sample are improper；
2, it can not be preferably used for histofluorescence dyeing, compared with the cell of culture, tissue samples to be increasingly complex, be through
Slice is crossed, the processes such as antigen retrieval combine sample properties and antibody and produce large effect, therefore studies in China is answered at present
It is less with histofluorescence staining technique.
Utility model content
(1) the technical issues of solving
In view of the deficiencies of the prior art, the utility model provides a kind of immunofluorescence dyeing kit, solves influence
The problem of factor is various and can not be applied to histofluorescence dyeing.
(2) technical solution
To achieve the above object, the utility model provides the following technical solutions: a kind of immunofluorescence dyeing kit, including
Box body, the surface of the box body are provided with the first reservoir, and the inside of first reservoir is placed with permeable membrane liquid, and described first
The box surface of reservoir side is provided with the second reservoir, and the inside of second reservoir is placed with fluorescence secondary antibody, described
The box surface of second reservoir side is provided with third reservoir, and the inside of the third reservoir is placed with the first reparation
Liquid, the box surface of third reservoir side are provided with the 4th reservoir, and the inside of the 4th reservoir is placed with
Two repair liquid, and the box surface of first reservoir one end is provided with the 5th reservoir, and the inside of the 5th reservoir is put
It is equipped with fixer, the box surface of the 5th reservoir side is provided with the 6th reservoir, the inside of the 6th reservoir
Be placed with cleaning solution, the box surface of the 6th reservoir side is provided with the 7th reservoir, the 7th reservoir it is interior
Portion is placed with confining liquid, and the box surface of the 7th reservoir side is provided with the 8th reservoir, the 8th reservoir
Inside is placed with mounting liquid.
Preferably, the internal diameter of the 5th reservoir, the 6th reservoir and the 7th reservoir is equal.
Preferably, the internal diameter of first reservoir, the second reservoir, third reservoir and the 4th reservoir is equal.
Preferably, the internal diameter of the 5th reservoir is greater than the internal diameter of the first reservoir, the internal diameter of first reservoir
Greater than the internal diameter of the 8th reservoir.
(3) beneficial effect
The utility model provides a kind of immunofluorescence dyeing kit, have it is following the utility model has the advantages that
(1) the utility model passes through setting fixer, cleaning solution, confining liquid, fluorescence secondary antibody, mounting liquid, box body, permeable membrane
Liquid makes the utility model that the optimal practical type of immunofluorescence dyeing and reagent concentration be determined, all actual classifications are put
The shortcomings that being put into box body, effectively avoiding immunofluorescence dyeing, and the experimental procedure of immunofluorescence dyeing has been determined, it will
Its procedure, immobilization develop four kinds of immunofluorescence dyeing kits, the type of fluorescence and excitation wavelength are fixed,
While simplifying the complexity of immunofluorescence dyeing application, main immunofluorescence dyeing method is covered, not only may be used
For single immunofluorescence dyeing, the fluorescent staining and positioning of two kinds of albumen can also be carried out, thus effective solution shadow
The various problem of the factor of sound, in the operation of the reagent progress immunofluorescence dyeing using tray interior, by cell climbing sheet from training
It supports and is taken out in base, after cleaning solution cleaning three times, fixer processing is added ten minutes later, recycles cleaning solution shaking table cleaning three
Secondary, three minutes every time, the sample drop after cleaning added confining liquid, is kept for 30 minutes at room temperature, gets rid of extra liquid, be sure not
Cleaning, then to specimen locations, 37 degree are incubated for one hour the primary antibody after dropwise addition dilution appropriate, are shaken again using cleaning solution
Bed cleans three times, and three minutes every time, and then immunofluorescence secondary antibody is added dropwise to specimen locations, 37 degree of incubations under the conditions of being protected from light
40 minutes, recycle the cleaning of cleaning solution shaking table three times, three minutes every time, whole process needs were carried out in the dark, and were utilizing room temperature
It is transparent by permeable membrane liquid after drying, fluorescence mounting liquid is then added dropwise and carries out mounting, entire immunofluorescence dyeing step is complete at this time
At utilizing micro- sem observation.
(2) the utility model repairs liquid, the second reparation liquid by setting cleaning solution, fixer, first, makes the utility model
For the immunofluorescence of tissue, the difficult point of histogenic immunity fluorescent staining is significantly overcome by optimizing, can be used for animal tissue
The fluorescent staining and positioning of single immunofluorescence and two kinds of albumen, have greatly pushed the application of immunofluorescence dyeing technology, from
And effective solution can not be applied to the problem of histofluorescence dyeing, it, will in the immunofluorescence dyeing operation for tissue
After tissue separation, three times using cleaning solution cleaning, fixer processing ten two to four is added 18 hours, paraffin packet is utilized after dehydration
Slice is buried, directly starts Seal treatment if without antigen retrieval at this time, if desired antigen retrieval, the utility model uses two
Kind restorative procedure, first, being added dropwise to specimen locations after first is repaired liquid boiling water bath eight minutes, 37 degree are incubated for five minutes,
Then it is repeated once this operation again, later use cleaning solution shaking table cleans twice, and three minutes every time, the method was that hot repair resists again
Original, second, being directly added dropwise second repairs liquid to specimen locations, 37 degree are incubated for 15 minutes, are cleaned using cleaning solution shaking table
Twice, every time three minutes, the method was that enzyme repairs antigen, can carry out subsequent Seal treatment after antigen retrieval.
Detailed description of the invention
Fig. 1 is the utility model schematic diagram of internal structure；
Fig. 2 is the utility model rear interior structural schematic diagram.
In figure: 1 first reservoir, 2 permeable membrane liquid, 3 second reservoirs, 4 fluorescence secondary antibodies, 5 third reservoirs, 6 first are repaired
Liquid, 9 the 5th reservoirs, 10 fixers, 11 the 6th reservoirs, 12 cleaning solutions, 13 the 7th are repaired in liquid, 7 the 4th reservoirs, 8 second
Reservoir, 14 confining liquids, 15 the 8th reservoirs, 16 mounting liquid, 17 box bodys.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
As shown in Figs. 1-2, the utility model provides a kind of technical solution: a kind of immunofluorescence dyeing kit, including box
Body 17, the surface of box body 17 are provided with the first reservoir 1, and the inside of the first reservoir 1 is placed with permeable membrane liquid 2, the first reservoir 1
17 surface of box body of side is provided with the second reservoir 3, and the inside of the second reservoir 3 is placed with fluorescence secondary antibody 4, the second reservoir
17 surface of box body of 3 sides is provided with third reservoir 5, and the inside of third reservoir 5 is placed with the first reparation liquid 6, third storage
17 surface of box body of 5 side of liquid bath is provided with the 4th reservoir 7, the first reservoir 1, the second reservoir 3,5 and of third reservoir
The internal diameter of 4th reservoir 7 is equal, and the inside of the 4th reservoir 7 is placed with the second reparation liquid 8, the box of 1 one end of the first reservoir
17 surface of body is provided with the 5th reservoir 9, and the inside of the 5th reservoir 9 is placed with fixer 10,9 side of the 5th reservoir
17 surface of box body is provided with the 6th reservoir 11, and the inside of the 6th reservoir 11 is placed with cleaning solution 12, in exempting from for tissue
When epidemic disease fluorescent staining operates, after tissue separation, three times using the cleaning of cleaning solution 12, the processing of fixer 10 ten two to four ten is added
Eight hours, specimens paraffin embedding slices are utilized after dehydration, directly start Seal treatment if without antigen retrieval at this time, if desired antigen
It repairs, the utility model uses two kinds of restorative procedures, first, being added dropwise to sample after first is repaired 6 boiling water bath of liquid eight minutes
Position, 37 degree are incubated for five minutes, are then repeated once this operation again, and 12 shaking table of later use cleaning solution cleans twice,
Three minutes every time, the method was that antigen is answered in hot repair, second, being directly added dropwise second repairs liquid 8 to specimen locations, 37 degree of incubations
15 minutes, twice using the cleaning of 12 shaking table of cleaning solution, three minutes every time, the method was that enzyme repairs antigen, was after antigen retrieval
Subsequent Seal treatment can be carried out, immunofluorescence of the utility model for tissue is made, tissue is significantly overcome by optimization and is exempted from
The difficult point of epidemic disease fluorescent staining can be used for the single immunofluorescence of animal tissue and the fluorescent staining and positioning of two kinds of albumen, greatly
Ground has pushed the application of immunofluorescence dyeing technology, and 17 surface of box body of 11 side of the 6th reservoir is provided with the 7th reservoir
13, the internal diameter of the 5th reservoir 9, the 6th reservoir 11 and the 7th reservoir 13 is equal, and the inside of the 7th reservoir 13 is placed with
Confining liquid 14,17 surface of box body of 13 side of the 7th reservoir are provided with the 8th reservoir 15, and the internal diameter of the 5th reservoir 9 is greater than
The internal diameter of first reservoir 1, the internal diameter of the first reservoir 1 are greater than the internal diameter of the 8th reservoir 15, the inside of the 8th reservoir 15
It is placed with mounting liquid 16, when carrying out the operation of immunofluorescence dyeing using the reagent inside box body 17, by cell climbing sheet from training
It supports and is taken out in base, after the cleaning three times of cleaning solution 12, the processing of fixer 10 is added ten minutes later, cleaning solution 12 is recycled to shake
Three times, three minutes every time, the sample drop after cleaning added confining liquid 14 for bed cleaning, was kept for 30 minutes at room temperature, it is extra to get rid of
Liquid, be sure not to clean, then the primary antibody appropriate being added dropwise after dilution is to specimen locations, 37 degree incubation one hour, again
Three times using the cleaning of 12 shaking table of cleaning solution, three minutes every time, and then immunofluorescence secondary antibody 4 is added dropwise to sample portion under the conditions of being protected from light
Position, 37 degree are incubated for 40 minutes, recycle the cleaning of 12 shaking table of cleaning solution three times, and three minutes every time, whole process needs were kept away
Light carries out, transparent by permeable membrane liquid 2 after using drying at room temperature, and fluorescence mounting liquid 16 is then added dropwise and carries out mounting, at this time entirely
Immunofluorescence dyeing step is completed, and using micro- sem observation, the utility model has determined the optimal reality of immunofluorescence dyeing
Border type and reagent concentration, all actual classifications are put in box body 17, effectively avoid lacking for immunofluorescence dyeing
Point, and the experimental procedure of immunofluorescence dyeing has been determined, by its procedure, immobilization develops four kinds of immunofluorescence dyeings
The type of fluorescence and excitation wavelength are fixed kit, are simplifying the same of the complexity of immunofluorescence dyeing application
When, main immunofluorescence dyeing method is covered, cannot be only used for single immunofluorescence dyeing, two hatching eggs can also be carried out
White fluorescent staining and positioning.
In use, the utility model using inside box body 17 reagent carry out immunofluorescence dyeing operation when, will be thin
Born of the same parents' creep plate takes out from culture medium, and after the cleaning three times of cleaning solution 12, the processing of fixer 10 is added ten minutes later, recycles clear
12 shaking table of washing lotion cleans three times, and the sample drop after cleaning adds confining liquid 14, keeps 30 minutes then drops appropriate at room temperature
To specimen locations, 37 degree are incubated for one hour primary antibody after adding dilution, utilize the cleaning of 12 shaking table of cleaning solution again three times, into
And immunofluorescence secondary antibody 4 is added dropwise under the conditions of being protected from light to specimen locations, 37 degree are incubated for 40 minutes, recycle cleaning solution 12
Shaking table cleans three times, transparent by permeable membrane liquid 2 after using drying at room temperature, and fluorescence mounting liquid 16 is then added dropwise and carries out mounting,
For tissue immunofluorescence dyeing operation when, will tissue separation after, using cleaning solution 12 cleaning three times, be added fixer 10
It handles ten two to four ten eight hours, specimens paraffin embedding slices is utilized after dehydration, directly start to close if without antigen retrieval at this time
Processing, if desired antigen retrieval, the utility model use two kinds of restorative procedures, first, the first reparation 6 boiling water bath eight of liquid is divided
Zhong Hou is added dropwise to specimen locations, and 37 degree are incubated for five minutes, is then repeated once this operation, later use cleaning solution again
12 shaking tables clean twice, and the method is that antigen answer in hot repair, second, directly the second reparation of dropwise addition liquid 8 is to specimen locations, 37 degree
It is incubated for 15 minutes, twice using the cleaning of 12 shaking table of cleaning solution, the method is that enzyme repairs antigen.
It can to sum up obtain, the utility model passes through setting fixer 10, cleaning solution 12, confining liquid 14, fluorescence secondary antibody 4, mounting
Liquid 16, box body 17, permeable membrane liquid 2, first repair liquid 6, second and repair 8 structure of liquid, and it is various and can not apply to solve influence factor
In histofluorescence dyeing the problem of.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (4)
1. a kind of immunofluorescence dyeing kit, including box body (17), it is characterised in that: the surface of the box body (17) is provided with
The inside of first reservoir (1), first reservoir (1) is placed with permeable membrane liquid (2), the first reservoir (1) side
Box body (17) surface is provided with the second reservoir (3), and the inside of second reservoir (3) is placed with fluorescence secondary antibody (4), described
Box body (17) surface of the second reservoir (3) side is provided with third reservoir (5), and the inside of the third reservoir (5) is put
It is equipped with the first reparation liquid (6), box body (17) surface of third reservoir (5) side is provided with the 4th reservoir (7), described
The inside of 4th reservoir (7) is placed with the second reparation liquid (8), and box body (17) surface of described first reservoir (1) one end is set
It is equipped with the 5th reservoir (9), the inside of the 5th reservoir (9) is placed with fixer (10), the 5th reservoir (9) one
Box body (17) surface of side is provided with the 6th reservoir (11), and the inside of the 6th reservoir (11) is placed with cleaning solution
(12), box body (17) surface of the 6th reservoir (11) side is provided with the 7th reservoir (13), the 7th reservoir
(13) inside is placed with confining liquid (14), and box body (17) surface of the 7th reservoir (13) side is provided with the 8th liquid storage
The inside of slot (15), the 8th reservoir (15) is placed with mounting liquid (16).
2. a kind of immunofluorescence dyeing kit according to claim 1, it is characterised in that: the 5th reservoir (9),
The internal diameter of 6th reservoir (11) and the 7th reservoir (13) is equal.
3. a kind of immunofluorescence dyeing kit according to claim 1, it is characterised in that: first reservoir (1),
The internal diameter of second reservoir (3), third reservoir (5) and the 4th reservoir (7) is equal.
4. a kind of immunofluorescence dyeing kit according to claim 1, it is characterised in that: the 5th reservoir (9)
Internal diameter be greater than the internal diameter of the first reservoir (1), the internal diameter of first reservoir (1) is greater than the interior of the 8th reservoir (15)
Diameter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820920636.1U CN208476787U (en) | 2018-06-14 | 2018-06-14 | A kind of immunofluorescence dyeing kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820920636.1U CN208476787U (en) | 2018-06-14 | 2018-06-14 | A kind of immunofluorescence dyeing kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208476787U true CN208476787U (en) | 2019-02-05 |
Family
ID=65213686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820920636.1U Expired - Fee Related CN208476787U (en) | 2018-06-14 | 2018-06-14 | A kind of immunofluorescence dyeing kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208476787U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683633A (en) * | 2021-01-19 | 2021-04-20 | 百盛(广州)生物制品有限公司 | Full-automatic immunohistochemical dyeing machine |
-
2018
- 2018-06-14 CN CN201820920636.1U patent/CN208476787U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683633A (en) * | 2021-01-19 | 2021-04-20 | 百盛(广州)生物制品有限公司 | Full-automatic immunohistochemical dyeing machine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fogh et al. | A method for direct demonstration of pleuropneumonia-like organisms in cultured cells. | |
| Brookes | A stain for differentiating two types of acidophil cells in the rat pituitary | |
| Herr | Practical nuclear staining procedures for Rhizoctonia-like fungi | |
| CN103710434B (en) | A kind of making method of marrow chromosome G band | |
| DE50010126D1 (en) | METHOD AND DEVICE FOR CHARACTERIZING A CULTURAL FLUID | |
| CN103926121B (en) | A kind of preparation method of cell climbing sheet | |
| CN208476787U (en) | A kind of immunofluorescence dyeing kit | |
| CN110398401A (en) | A kind of method of Thinprep pap test film-making | |
| CN201737950U (en) | Special experimental apparatus for cell growing on cover glass | |
| CN103884560A (en) | Preparation method and application of Chinese giant salamander phalanx section | |
| CN102043047B (en) | Rapid and economical immunofluorescence method based on Fisher | |
| CN102636646A (en) | Test paper strip for rapidly detecting avian leukosis antigen and preparation method thereof | |
| CN110174516A (en) | Goose parvovirus VP3 proteantigen ELISA detection kit and detection method and application | |
| CN104949876A (en) | Detachable, volume-adjustable and space-sealed glass slide incubator reasonable in structure | |
| ATE112631T1 (en) | ARTICLE FOR CARRYING OUT IMMUNOTESTS USING ORGANIC DYES, AND METHODS FOR ITS PREPARATION AND ITS USE. | |
| CN104614516A (en) | Slide incubator ensuring good experimental results | |
| CN103667183B (en) | Bone marrow cell cultures | |
| CN108925511A (en) | Tachypleus tridentatus young Ecology cultivation apparatus | |
| CN101245315B (en) | Cultivation device and method for adherent cell thin membrane of laboratory | |
| CN105699141B (en) | A kind of tabletting method of eucalyptus chromosome | |
| CN208783574U (en) | Tachypleus tridentatus young Ecology cultivation apparatus | |
| Gifford | Isolation and laboratory culture of marine oligotrichous ciliates | |
| CN106092991A (en) | A kind of utilize DAPI fluorescent dye to the detection method of active o content in living cells | |
| CN113984483B (en) | Method for staining cover glass patch of frozen section | |
| CN109187970A (en) | It is a kind of quickly to detect aquatic products disease gold mark nucleic acid test strip and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190205 Termination date: 20190614 |