DE50010126D1 - METHOD AND DEVICE FOR CHARACTERIZING A CULTURAL FLUID - Google Patents

METHOD AND DEVICE FOR CHARACTERIZING A CULTURAL FLUID

Info

Publication number
DE50010126D1
DE50010126D1 DE50010126T DE50010126T DE50010126D1 DE 50010126 D1 DE50010126 D1 DE 50010126D1 DE 50010126 T DE50010126 T DE 50010126T DE 50010126 T DE50010126 T DE 50010126T DE 50010126 D1 DE50010126 D1 DE 50010126D1
Authority
DE
Germany
Prior art keywords
culture liquid
characterizing
cells
comparison
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE50010126T
Other languages
German (de)
Inventor
Christoph Bittner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
innovatis AG
Original Assignee
innovatis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by innovatis AG filed Critical innovatis AG
Priority to DE50010126T priority Critical patent/DE50010126D1/en
Application granted granted Critical
Publication of DE50010126D1 publication Critical patent/DE50010126D1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/21—Polarisation-affecting properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation

Abstract

In a method for characterizing a culture liquid, in particular in a bioreactor, by illuminating the cells ( 22 ) contained in the culture liquid the culture liquid is analyzed in situ, microscopic imaging of the cells ( 22 ) and by evaluating the image. The illumination is carried out as dark-field illumination, and the image evaluation comprises a comparison of the intensities of the light output by the interior of the cell and by the edge of the cell, by means of which comparison living and dead cells ( 22 ) are distinguished in order to determine the vitality of the culture liquid.
DE50010126T 1999-10-11 2000-10-11 METHOD AND DEVICE FOR CHARACTERIZING A CULTURAL FLUID Expired - Lifetime DE50010126D1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE50010126T DE50010126D1 (en) 1999-10-11 2000-10-11 METHOD AND DEVICE FOR CHARACTERIZING A CULTURAL FLUID

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19949029A DE19949029C2 (en) 1999-10-11 1999-10-11 Method and device for characterizing a culture fluid
PCT/EP2000/010030 WO2001027591A2 (en) 1999-10-11 2000-10-11 Method and device for characterizing a culture liquid
DE50010126T DE50010126D1 (en) 1999-10-11 2000-10-11 METHOD AND DEVICE FOR CHARACTERIZING A CULTURAL FLUID

Publications (1)

Publication Number Publication Date
DE50010126D1 true DE50010126D1 (en) 2005-05-25

Family

ID=7925287

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19949029A Expired - Fee Related DE19949029C2 (en) 1999-10-11 1999-10-11 Method and device for characterizing a culture fluid
DE50010126T Expired - Lifetime DE50010126D1 (en) 1999-10-11 2000-10-11 METHOD AND DEVICE FOR CHARACTERIZING A CULTURAL FLUID

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE19949029A Expired - Fee Related DE19949029C2 (en) 1999-10-11 1999-10-11 Method and device for characterizing a culture fluid

Country Status (8)

Country Link
US (1) US6905838B1 (en)
EP (1) EP1248947B1 (en)
JP (1) JP2003529747A (en)
CN (1) CN1181334C (en)
AT (1) ATE293787T1 (en)
AU (1) AU7918700A (en)
DE (2) DE19949029C2 (en)
WO (1) WO2001027591A2 (en)

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DE102012101086A1 (en) 2011-02-10 2012-08-16 Presens Precision Sensing Gmbh Device for detecting image of sample or for detecting variable of sample, comprises container for sample, and sensor in container, where sensor indicates optical behavior corresponding to variable
DE102012205311B4 (en) * 2012-03-30 2013-10-17 Anton Paar Gmbh Optical device, in particular polarimeter, for detecting inhomogeneities in a sample
US20150087902A1 (en) * 2012-03-30 2015-03-26 Trustees Of Boston University Phase Contrast Microscopy With Oblique Back-Illumination
JP6255013B2 (en) 2012-06-18 2017-12-27 グリーンオニキス リミテッド A compact device for the continuous production of product from starting materials grown under aquaculture conditions
CN104885187B (en) 2012-10-30 2018-06-29 加州理工学院 Fourier overlapping associations imaging system, device and method
US9864184B2 (en) 2012-10-30 2018-01-09 California Institute Of Technology Embedded pupil function recovery for fourier ptychographic imaging devices
US10652444B2 (en) 2012-10-30 2020-05-12 California Institute Of Technology Multiplexed Fourier ptychography imaging systems and methods
FR3009084B1 (en) * 2013-07-23 2015-08-07 Commissariat Energie Atomique METHOD FOR SORTING CELLS AND ASSOCIATED DEVICE
CA2919985A1 (en) 2013-07-31 2015-02-05 California Institute Of Technology Aperture scanning fourier ptychographic imaging
WO2015027188A1 (en) 2013-08-22 2015-02-26 California Institute Of Technoloby Variable-illumination fourier ptychographic imaging devices, systems, and methods
US9274071B2 (en) 2013-12-30 2016-03-01 General Electric Company Methods for assessing cell culture fluid by impedance spectra
CN103823025B (en) * 2014-03-04 2016-02-10 浙江大学宁波理工学院 Antifreeze solution for cell adds or takes out program screening plant
US10039244B2 (en) 2014-03-04 2018-08-07 Greenonyx Ltd Systems and methods for cultivating and distributing aquatic organisms
US11468557B2 (en) 2014-03-13 2022-10-11 California Institute Of Technology Free orientation fourier camera
US10162161B2 (en) 2014-05-13 2018-12-25 California Institute Of Technology Ptychography imaging systems and methods with convex relaxation
CN105445227B (en) * 2014-08-29 2019-04-02 清华大学 A kind of method and device for observing monodimension nanometer material
EP3238135B1 (en) 2014-12-22 2020-02-05 California Institute Of Technology Epi-illumination fourier ptychographic imaging for thick samples
EP3248208B1 (en) 2015-01-21 2019-11-27 California Institute of Technology Fourier ptychographic tomography
EP3250908A4 (en) 2015-01-26 2018-09-12 California Institute of Technology Multi-well fourier ptychographic and fluorescence imaging
AU2016233588A1 (en) 2015-03-13 2017-10-26 California Institute Of Technology Correcting for aberrations in incoherent imaging system using fourier ptychographic techniques
US9993149B2 (en) 2015-03-25 2018-06-12 California Institute Of Technology Fourier ptychographic retinal imaging methods and systems
WO2016187591A1 (en) 2015-05-21 2016-11-24 California Institute Of Technology Laser-based fourier ptychographic imaging systems and methods
CN108431664B (en) * 2015-12-23 2021-11-02 皇家飞利浦有限公司 Fluorescent calibration slide
US11002667B2 (en) 2015-12-23 2021-05-11 Koninklijke Philips N.V. Calibration slide for digital pathology
WO2017147528A1 (en) * 2016-02-26 2017-08-31 University Of Southern California Optimized volumetric imaging with selective volume illumination and light field detection
JP2017161740A (en) * 2016-03-10 2017-09-14 オリンパス株式会社 Light field microscope
US11092795B2 (en) 2016-06-10 2021-08-17 California Institute Of Technology Systems and methods for coded-aperture-based correction of aberration obtained from Fourier ptychography
US10568507B2 (en) 2016-06-10 2020-02-25 California Institute Of Technology Pupil ptychography methods and systems
CN107645625B (en) * 2016-07-22 2020-10-09 松下知识产权经营株式会社 Image generation device and image generation method
DE102016012585A1 (en) * 2016-10-21 2018-04-26 Seidenader Maschinenbau Gmbh Device for detecting air bubbles in a container filled with liquid
JP7026333B2 (en) 2017-03-01 2022-02-28 パナソニックＩｐマネジメント株式会社 Image generator and image generation method
WO2019090149A1 (en) 2017-11-03 2019-05-09 California Institute Of Technology Parallel digital imaging acquisition and restoration methods and systems
CN109870424B (en) * 2019-03-05 2021-05-11 中国计量大学 Hartmann ray tracing method based on color three-step phase shifting technology
CN112268505B (en) * 2020-10-22 2022-11-08 江苏集萃微纳自动化系统与装备技术研究所有限公司 Microscopic operation system based on full-field optical coherence tomography technology
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Also Published As

Publication number Publication date
WO2001027591A2 (en) 2001-04-19
CN1399717A (en) 2003-02-26
JP2003529747A (en) 2003-10-07
EP1248947A2 (en) 2002-10-16
US6905838B1 (en) 2005-06-14
DE19949029A1 (en) 2001-05-17
ATE293787T1 (en) 2005-05-15
EP1248947B1 (en) 2005-04-20
WO2001027591A3 (en) 2002-08-01
CN1181334C (en) 2004-12-22
AU7918700A (en) 2001-04-23
DE19949029C2 (en) 2002-11-21

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