CN111272992A - Large flux antigen restoring instrument - Google Patents
Large flux antigen restoring instrument Download PDFInfo
- Publication number
- CN111272992A CN111272992A CN202010130477.7A CN202010130477A CN111272992A CN 111272992 A CN111272992 A CN 111272992A CN 202010130477 A CN202010130477 A CN 202010130477A CN 111272992 A CN111272992 A CN 111272992A
- Authority
- CN
- China
- Prior art keywords
- water bath
- flux
- water
- antigen retrieval
- processing module
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
Abstract
The invention belongs to the technical field of antigen retrieval equipment, and particularly relates to a large-flux antigen retrieval instrument which comprises a water bath box body, wherein a water bath heating groove is formed in the inner side of the water bath box body, an automatic high-pressure sealing cover is connected to a rotating shaft at the top of the water bath box body, a plurality of placing tables are arranged in the water bath heating groove, a multi-connected slide glass placing frame is placed on each placing table, a heating coil, a temperature probe and a water inlet are arranged on the side surface of the water bath heating groove, a water outlet is formed in the bottom of the water bath heating groove, a control key and an information display screen are arranged on the front surface of the water bath box body, the heating coil, the temperature probe, the control key and the information display screen are. The invention designs automatic top cover, water pump, rack for placing large amount of glass slide in water bath device, which can effectively improve antigen repairing efficiency, with simple operation and remarkable substantive characteristics.
Description
Technical Field
The invention relates to the technical field of antigen retrieval equipment, in particular to a large-flux antigen retrieval instrument.
Background
With the increasing incidence of tumor, the immunohistochemical staining experiment used for tumor diagnosis is on the rising trend year by year, and the annual composite growth rate is 15%. The tissue heat repairing method in the immunohistochemical experiment comprises the following steps: the method mainly comprises the means of an electric baking method, a water bath heating method, a microwave method and the like, and the electric baking method has the defects of complex heating device, high failure rate, high cost, easiness in drying, small quantity of heating slices in a single time and the like; the water bath heating method is simple and easy to implement, but the automatic module (no finished product is available) of full-automatic immunohistochemical dyeing cannot be made, a single pressure cooker is required for heating, the efficiency is low, and the piece is easy to fall; the microwave heating method has the defects of uneven heating, easy drying, poor heat repairing effect and gradual elimination.
In order to improve the accuracy and the treatment efficiency of an immunohistochemical staining experiment, the development of the water bath heating method antigen retrieval equipment which is high in integration degree and convenient to use is necessary.
Disclosure of Invention
In order to solve the problem that the existing water bath heating method for antigen restoration lacks special experimental equipment, the traditional restoration method has lower treatment efficiency, complex manual operation and high accuracy, is easily influenced by misoperation and cannot meet the increasing requirements of immunohistochemical staining experiments, a large-flux antigen restoration instrument is provided.
Big flux antigen retrieval appearance, including the water bath box, water bath box inboard is provided with the water bath heating tank, water bath box top pivot is connected with automatic high-pressure sealing lid, be provided with a plurality of platforms of placing in the water bath heating tank, place the bench and all placed and ally oneself with slide glass rack, water bath heating tank side is provided with heating coil, temperature probe and water inlet, and water bath heating tank bottom is provided with the outlet, and the water bath box openly is provided with control button and information display screen, the equal electric connection of heating coil, temperature probe, control button and information display screen has processing module, the upper reaches of water inlet still water route intercommunication has the water pump, water pump electric connection processing module.
Further, the multi-connected glass slide placing rack comprises a supporting base plate, a plurality of glass slide fixing grooves are formed in the supporting base plate in an equidistant arrangement mode, glass slide supports are vertically placed in the glass slide fixing grooves, and vertical supporting plates are arranged on one side of the bottoms of the glass slide fixing grooves.
Furthermore, the glass slide support comprises a clamping plate with the rear end inclined downwards, clamping plate side walls are symmetrically arranged on the left side and the right side of the clamping plate, and glass slide clamping grooves are formed in the inner sides of the clamping plate side walls.
Further, the side of the water bath box body is provided with an electric push rod, the movable end of the electric push rod is fixedly connected with the side of the automatic high-pressure sealing cover, and the electric push rod is electrically connected with the processing module.
Furthermore, a cooling fan is arranged on the back of the water bath box body and electrically connected with the processing module.
Further, the bottom of the water bath heating groove is communicated with a funnel-shaped water draining seat, and an outlet of the funnel-shaped water draining seat is communicated with a water draining port.
Furthermore, a three-way electromagnetic valve is communicated with the lower part of the water outlet and is electrically connected with the processing module.
Further, a pressure relief opening is arranged on the automatic high-pressure sealing cover in a penetrating mode.
Furthermore, a single chip microcomputer chip is arranged on the processing module.
Further, the temperature probe is a PT1000 temperature sensor.
The invention has the advantages that:
1. the integration degree is high, and a large number of glass slides can be placed at one time for batch processing.
2. Convenient to use adopts the water pump to add water automatically, has reduced operating personnel's work load.
3. The heating time and the heating temperature can be set by utilizing a control button, and the automatic operation is realized.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a perspective view of the construction of a high flux antigen retrieval instrument;
FIG. 2 is a schematic view of a slide mount configuration;
FIG. 3 is a schematic structural diagram of the multiple slide rack with slide racks;
FIG. 4 is a schematic block diagram of the circuit of the large flux antigen retrieval instrument.
The attached drawings are as follows:
1-a water bath box body, 2-a water bath heating groove, 21-a funnel-shaped drainage seat, 3-an automatic high-pressure sealing cover, 31-a pressure relief hole, 4-a placing table, 5-a multi-slide placing frame, 51-a supporting bottom plate, 52-a slide fixing groove, 53-a slide support, 531-a clamping plate, 532-a clamping plate side wall, 533-a slide clamping groove, 54-a vertical supporting plate, 6-a heating coil, 7-a temperature probe, 8-a water inlet, 81-a water outlet, 9-a control key, 10-an information display screen, 11-a processing module, 111-a single chip microcomputer chip, 12-a water pump, 13-an electric push rod, 14-a cooling fan and 15-a three-way electromagnetic valve.
Detailed Description
In order to solve the problem that the existing water bath heating method for antigen restoration lacks special experimental equipment, the traditional restoration method has lower treatment efficiency, complex manual operation and high accuracy, is easily influenced by misoperation and cannot meet the increasing requirements of immunohistochemical staining experiments, a large-flux antigen restoration instrument is provided.
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-4, this embodiment provides a large-flux antigen retrieval instrument, including water bath box 1, 1 inboard of water bath box is provided with water bath heating tank 2, 1 top pivot of water bath box is connected with automatic high-pressure sealing lid 3, be provided with a plurality of platforms 4 of placing in the water bath heating tank 2, all placed on the platform 4 and ally oneself with slide glass rack 5 more, water bath heating tank 2 side is provided with heating coil 6, temperature probe 7 and water inlet 8, water bath heating tank 2 bottom is provided with outlet 81, and water bath box 1 openly is provided with control button 9 and information display screen 10, the equal electric connection of heating coil 6, temperature probe 7, control button 9 and information display screen 10 has processing module 11, the water route intercommunication in the upper reaches of water inlet 8 has water pump 12, water pump 12 electric connection processing module 11. During the use, will place the multiple slide glass rack 5 of putting the slide glass and place and be good on placing platform 4, set for through control button 9 with water yield, temperature and water bath time after, can start equipment. Then the automatic high-pressure sealing cover 3 is closed, the water pump 12 pumps the set water quantity into the water bath heating tank 2 through the water inlet 8, and the heating coil 6 is opened to heat the water, so that the antigen repairing process is carried out. After the set time, the heating is stopped, the automatic high-pressure sealing cover 3 is opened, and the waste water can be discharged through the water outlet 8.
The multi-connected glass slide placing rack 5 comprises a supporting base plate 51, a plurality of glass slide fixing grooves 52 are arranged on the supporting base plate 51 at equal intervals, glass slide supports 53 are vertically placed in the glass slide fixing grooves 52, and vertical supporting plates 54 are arranged on one side of the bottoms of the glass slide fixing grooves 52. A slide is placed on each slide rack 53, and the support base 51 having a plurality of slide holding grooves 52 can be placed on a plurality of vertically placed slide racks 53 at a time, so that a large number of slides can be loaded at a time. The upright support plate 54 provides support for the vertically positioned slide support 53 for improved stability.
The slide support 53 comprises a clamping plate 531 with a downward inclined rear end, clamping plate side walls 532 are symmetrically arranged on the left side and the right side of the clamping plate 531, and slide clamping grooves 533 are arranged on the inner sides of the clamping plate side walls 532. The slide can be clamped into the slide clamping groove 533 for fixed placement, and the slide support 53 facilitates the movement and taking of the slide for use.
The side of the water bath box body 1 is provided with an electric push rod 13, the movable end of the electric push rod 13 is fixedly connected with the side of the automatic high-pressure sealing cover 3, and the electric push rod 13 is electrically connected with the processing module 11. The electric push rod 13 can drive the automatic high-pressure sealing cover 3 to automatically open and close under the control of the processing module 11, and the automatic high-pressure sealing cover is closed when the processing is started and automatically opened after the processing is finished, so that the convenience of the equipment is improved.
The back of the water bath box body 1 is provided with a cooling fan 14, and the cooling fan 14 is electrically connected with the processing module 11. The cooling fan 14 is used for improving the cooling efficiency of the equipment and avoiding the overhigh temperature when the equipment runs.
The bottom of the water bath heating groove 2 is communicated with a funnel-shaped drainage seat 21, and an outlet of the funnel-shaped drainage seat 21 is communicated with a drainage outlet 8. The funnel-shaped bottom of the water bath heating tank 2 can improve the drainage rate and avoid the residual wastewater.
A three-way electromagnetic valve 15 is communicated below the water outlet 8, and the three-way electromagnetic valve 15 is electrically connected with the processing module 11. The other two ends of the three-way electromagnetic valve 15 are respectively connected with different pipelines, and the opening time of each valve is controlled by the processing module 11, so that the experimental wastewater can be separated, collected and processed, and the waste liquid treatment cost is reduced.
The automatic high-pressure sealing cover 3 is provided with a pressure relief opening 31 in a penetrating mode. The pressure relief opening 31 can automatically exhaust and relieve pressure when the air pressure in the water bath heating tank 2 is too high, so that the operation safety of the equipment is ensured.
The processing module 11 is provided with a single chip microcomputer chip 111. The single chip 111 may be a commercially available STM32 single chip, and the processing module 11 is an integrated circuit board carrying an STM32 single chip. Since the single chip microcomputer circuit board based on STM32 is a common technical means in the field (refer to 'STM 32 hardware circuit design', link: https:// wenku.baidu.com/view/2254b2b85ef7b 0d4b733b1b. html), the single chip microcomputer circuit board adopted in the scheme is also a conventionally designed single chip microcomputer circuit board, and does not relate to new design of a control circuit, so that details are not described. In actual operation, an analog signal detected by the temperature probe 7 is subjected to a/D conversion (i.e., a digital-to-analog conversion module commonly used in the field) and then received by the single chip microcomputer chip 111, and is displayed by the information display screen 10, and then the single chip microcomputer chip 111 controls the operating power of the heating coil 6 after a control signal is subjected to D/a conversion (i.e., a digital-to-analog conversion module commonly used in the field) according to a set temperature value, so as to keep the water bath temperature at a set value. In addition, digital control signals sent by the single chip microcomputer chip 111 are all received by the water pump 12, the electric push rod 13, the cooling fan 14 and the three-way electromagnetic valve 15 after being subjected to D/A conversion, and therefore the automatic control effect is achieved.
The specific automatic control functions are as follows: 1. the user sets up corresponding water yield through control button 9 according to the sample quantity that needs were handled, and control water pump 12 is automatic to be added water, need not artifical the weighing. 2. The electric push rod 13 can drive the automatic high-pressure sealing cover 3 to close at the beginning of the water bath and open after the water bath is finished. 3. The cooling fan 14 can be turned on when the system detects that the temperature is too high, so as to increase the cooling speed. 4. The other two ends of the three-way electromagnetic valve 15 are respectively connected with different pipelines, and the opening time of each valve is controlled by the processing module 11, so that the experimental wastewater can be separated, collected and processed, and the waste liquid treatment cost is reduced.
The temperature probe 7 is a PT1000 temperature sensor. The PT1000 is a common platinum thermal resistor in the market, the resistance value of the platinum thermal resistor is in direct proportion to the change of temperature, and the platinum thermal resistor has the advantage of higher precision.
The foregoing is a more detailed description of the invention than is specifically described in connection with the preferred embodiments, and it is not intended to limit the invention to the particular forms disclosed, since all equivalent variations and modifications as fall within the scope of the appended claims are intended to be embraced therein.
Claims (10)
1. Big flux antigen retrieval appearance, including the water bath box, water bath box inboard is provided with the water bath heating tank, its characterized in that, water bath box top pivot is connected with automatic high-pressure sealed lid, be provided with a plurality of platforms of placing in the water bath heating tank, place the bench and all placed and ally oneself with slide glass rack, water bath heating tank side is provided with heating coil, temperature probe and water inlet, and water bath heating tank bottom is provided with the outlet, and the water bath box openly is provided with control button and information display screen, the equal electric connection of heating coil, temperature probe, control button and information display screen has processing module, the upper reaches of water inlet are gone back the water route intercommunication and are had the water pump, water pump electric connection processing module.
2. The large-flux antigen retrieval instrument according to claim 1, wherein the multi-linked slide glass rack comprises a support base plate, a plurality of slide glass fixing grooves are arranged on the support base plate at equal intervals, slide glass supports are vertically arranged in the slide glass fixing grooves, and vertical support plates are arranged on one sides of bottoms of the slide glass fixing grooves.
3. The high-flux antigen retrieval instrument according to claim 2, wherein the slide holder comprises a clamping plate with a downward inclined rear end, the clamping plate is symmetrically provided with clamping plate side walls at the left and right sides, and slide slots are arranged at the inner sides of the clamping plate side walls.
4. The large-flux antigen retrieval instrument according to claim 1, wherein an electric push rod is installed on a side surface of the water bath box body, a movable end of the electric push rod is fixedly connected with a side surface of the automatic high-pressure sealing cover, and the electric push rod is electrically connected with the processing module.
5. The high-flux antigen retrieval instrument according to claim 1, wherein a heat dissipation fan is disposed on the back of the water bath box, and the heat dissipation fan is electrically connected to the processing module.
6. The high-flux antigen retrieval instrument according to claim 1, wherein the bottom of the water bath heating tank is communicated with a funnel-shaped drainage seat, and an outlet of the funnel-shaped drainage seat is communicated with a drainage outlet.
7. The high-flux antigen retrieval instrument according to claim 1, wherein a three-way solenoid valve is connected to a lower portion of the water outlet, and the three-way solenoid valve is electrically connected to the processing module.
8. The high flux antigen retrieval instrument of claim 1, wherein the automatic high pressure sealing cover is provided with a pressure relief opening.
9. The large-flux antigen retrieval instrument according to claim 1, wherein the processing module is provided with a single chip microcomputer chip.
10. The high flux antigen retrieval instrument of claim 1, wherein the temperature probe is a PT1000 temperature sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010130477.7A CN111272992A (en) | 2020-02-28 | 2020-02-28 | Large flux antigen restoring instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010130477.7A CN111272992A (en) | 2020-02-28 | 2020-02-28 | Large flux antigen restoring instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111272992A true CN111272992A (en) | 2020-06-12 |
Family
ID=71000482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010130477.7A Pending CN111272992A (en) | 2020-02-28 | 2020-02-28 | Large flux antigen restoring instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111272992A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683633A (en) * | 2021-01-19 | 2021-04-20 | 百盛(广州)生物制品有限公司 | Full-automatic immunohistochemical dyeing machine |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001004634A1 (en) * | 1999-07-08 | 2001-01-18 | Lee Angros | Antigen recovery and/or staining apparatus and method |
| CN202676526U (en) * | 2012-06-29 | 2013-01-16 | 广州安必平自动化检测设备有限公司 | Automatic antigen repair machine |
| CN205731307U (en) * | 2016-07-12 | 2016-11-30 | 佛山市海圆圆农产品科技有限公司 | A kind of intelligence thermostat water bath automatically |
| CN106226141A (en) * | 2016-03-21 | 2016-12-14 | 广州深达生物制品技术有限公司 | A kind of slide glass cover plate for slide glass sample disposal and using method thereof |
| CN206557229U (en) * | 2017-03-14 | 2017-10-13 | 中国人民解放军第三军医大学 | A kind of histotomy immune response incubating device |
| CN107583596A (en) * | 2017-11-01 | 2018-01-16 | 湖北耀楚医疗器械科技有限公司 | A kind of Biochemistry Experiment heating in water bath for reaction device |
| CN207187799U (en) * | 2017-09-12 | 2018-04-06 | 北京旌准医疗科技有限公司 | A kind of object slide stand |
| CN209311166U (en) * | 2018-10-18 | 2019-08-27 | 广州安必平医药科技股份有限公司 | Reaction warehouse and antigen retrieval instrument |
| CN110411810A (en) * | 2019-09-04 | 2019-11-05 | 深圳褀氏生物医疗电子有限公司 | A kind of immunohistochemical staining machine and immunohistochemical staining method |
| CN209802816U (en) * | 2019-03-29 | 2019-12-17 | 沈阳市第四人民医院 | Antigen retrieval device for cell retrieval |
| CN209985442U (en) * | 2019-05-15 | 2020-01-24 | 希肯医疗技术（苏州）有限公司 | Constant-temperature water bath kettle |
-
2020
- 2020-02-28 CN CN202010130477.7A patent/CN111272992A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001004634A1 (en) * | 1999-07-08 | 2001-01-18 | Lee Angros | Antigen recovery and/or staining apparatus and method |
| CN202676526U (en) * | 2012-06-29 | 2013-01-16 | 广州安必平自动化检测设备有限公司 | Automatic antigen repair machine |
| CN106226141A (en) * | 2016-03-21 | 2016-12-14 | 广州深达生物制品技术有限公司 | A kind of slide glass cover plate for slide glass sample disposal and using method thereof |
| CN205731307U (en) * | 2016-07-12 | 2016-11-30 | 佛山市海圆圆农产品科技有限公司 | A kind of intelligence thermostat water bath automatically |
| CN206557229U (en) * | 2017-03-14 | 2017-10-13 | 中国人民解放军第三军医大学 | A kind of histotomy immune response incubating device |
| CN207187799U (en) * | 2017-09-12 | 2018-04-06 | 北京旌准医疗科技有限公司 | A kind of object slide stand |
| CN107583596A (en) * | 2017-11-01 | 2018-01-16 | 湖北耀楚医疗器械科技有限公司 | A kind of Biochemistry Experiment heating in water bath for reaction device |
| CN209311166U (en) * | 2018-10-18 | 2019-08-27 | 广州安必平医药科技股份有限公司 | Reaction warehouse and antigen retrieval instrument |
| CN209802816U (en) * | 2019-03-29 | 2019-12-17 | 沈阳市第四人民医院 | Antigen retrieval device for cell retrieval |
| CN209985442U (en) * | 2019-05-15 | 2020-01-24 | 希肯医疗技术（苏州）有限公司 | Constant-temperature water bath kettle |
| CN110411810A (en) * | 2019-09-04 | 2019-11-05 | 深圳褀氏生物医疗电子有限公司 | A kind of immunohistochemical staining machine and immunohistochemical staining method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683633A (en) * | 2021-01-19 | 2021-04-20 | 百盛(广州)生物制品有限公司 | Full-automatic immunohistochemical dyeing machine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111272992A (en) | Large flux antigen restoring instrument | |
| CN210809012U (en) | Baking device for aquatic product processing | |
| CN111035157A (en) | Intelligent cabinet | |
| CN109757968A (en) | A kind of smokeless hot pot device | |
| CN2861803Y (en) | Electromagnetic tea infusion furnace | |
| CN113397158A (en) | Automatic production line applied to bean jelly and bean jelly production method | |
| CN218186132U (en) | Multifunctional heat-preservation meal selling table | |
| CN207707155U (en) | A kind of food processing continuous frying machine | |
| CN215016047U (en) | Electric heating pot capable of cleaning water stain on outer ring of inner container | |
| CN209914953U (en) | Machine for semi-automatically making brown rice tea | |
| CN207114293U (en) | A kind of endocrine electron microscope | |
| CN210433315U (en) | Jacking type lifting cooking box | |
| CN215686534U (en) | Multifunctional tea tray | |
| CN2823850Y (en) | Electromagnetic oven | |
| CN217772106U (en) | Whole dish heat preservation dining-table that generates heat | |
| CN220608103U (en) | Novel automatic water supplementing and lifting multifunctional noodle boiling machine | |
| CN213663390U (en) | Biscuit high-utilization-rate cyclic fermentation control device | |
| CN205625731U (en) | Support device of for kitchen use | |
| CN214104123U (en) | Speed-variable movable jacketed kettle | |
| CN219594307U (en) | Integrated machine for boiling noodles and adding soup | |
| CN214962435U (en) | Corn cooking machine | |
| CN217792513U (en) | Steamer | |
| CN220175624U (en) | Sterilizing cabinet for Chinese patent medicine | |
| CN214952508U (en) | Equipment for improving HE slice pasting technology | |
| CN217359240U (en) | Machine for mounting paraffin section |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200612 |