CN217173687U

CN217173687U - Closed card box for gene sequencing library construction

Info

Publication number: CN217173687U
Application number: CN202220589690.9U
Authority: CN
Inventors: 宿浩然
Original assignee: Beijing Fuying Kechuang Biotechnology Co Ltd
Current assignee: Beijing Fuying Kechuang Biotechnology Co Ltd
Priority date: 2022-03-17
Filing date: 2022-03-17
Publication date: 2022-08-12
Anticipated expiration: 2032-03-17

Abstract

The application discloses a closed type card box for gene sequencing library construction, which relates to the technical field of gene sequencing library construction and comprises a reagent tray and a shell, wherein the shell is detachably arranged on the reagent tray, a plurality of reagent grooves are arranged on the reagent tray, and each reagent groove is positioned in the shell; the shell is provided with a sample adding port for adding a sample to be built and a sampling port for taking out a library; the shell is also internally provided with a magnetic attraction component and a moving component for moving the magnetic attraction component, and the magnetic attraction component is arranged on the moving component in a transmission manner. The method and the device have the effects of realizing automatic library building and reducing the possibility of sample pollution during library building.

Description

Closed card box for gene sequencing library construction

Technical Field

The application relates to the technical field of gene sequencing and library construction, in particular to a closed type card box for gene sequencing and library construction.

Background

Gene sequencing is a novel gene detection technology, and can analyze and determine gene sequences from samples such as blood or saliva, and further predict the possibility of suffering from a certain disease and other physiological and pathological characteristics.

Gene sequencing and library building are to amplify specific DNA fragments by using polymerase chain reaction, which can be regarded as special DNA replication in vitro.

In the library preparation process of the traditional gene sequencing, an open pipetting system is usually used for completing a pipetting workflow, and the open library construction system is easy to cause cross contamination and is accompanied by the possibility of contaminating a sample, so that the gene sequencing result generates errors.

SUMMERY OF THE UTILITY MODEL

In order to reduce the possibility of sample pollution during library construction, the application provides a closed cassette for gene sequencing library construction.

The application provides a gene sequencing builds storehouse and uses closed card box adopts following technical scheme:

a closed card box for gene sequencing library building comprises a reagent tray and a shell, wherein the shell is detachably arranged on the reagent tray, the reagent tray is provided with a plurality of reagent grooves, and each reagent groove is positioned in the shell;

the shell is provided with a sample adding port for adding a sample to be built and a sampling port for taking out a library;

the magnetic attraction type magnetic control device is characterized in that a magnetic attraction component and a moving component used for moving the magnetic attraction component are further arranged in the shell, and the magnetic attraction component is arranged on the moving component in a transmission mode.

By adopting the technical scheme, when the DNA sample needs to be subjected to gene sequencing and library building, the reaction liquid is firstly added in each reagent groove of the reagent tray, and the shell is covered on the reagent tray. And putting the sample to be stored into the idle reagent groove of the reagent tray through the sample adding port. And finally, driving the magnetic suction assembly to perform gene sequencing library building operation among the test tubes through the moving assembly. The library after the library establishment can be taken out from a sampling port. The whole warehouse building operation is carried out in the shell, so that the possibility of sample pollution during warehouse building is reduced.

Optionally, the magnetic attraction component is arranged in a plurality of numbers, and the magnetic attraction component is arranged on the moving component in a transmission manner.

Through adopting above-mentioned technical scheme, magnetism is inhaled the subassembly many times and is made the card box build the storehouse when can carry out the multiunit sample to the storehouse efficiency of building when having improved the multiunit sample and having built the storehouse.

Optionally, the moving assembly comprises a vertical moving component and a horizontal moving component, and the moving direction of the horizontal moving component is perpendicular to the moving direction of the vertical moving component.

Through adopting above-mentioned technical scheme, lateral shifting part and vertical moving part make magnetism inhale the subassembly and can slide along reagent tray length direction to reciprocate, thereby make magnetism inhale the subassembly and can stretch into each reagent inslot on the reagent tray.

Optionally, the transverse moving component is a screw rod module, the screw rod module is fixedly arranged on the inner side wall of the shell, and the transmission direction of the screw rod module is perpendicular to the moving direction of the vertical moving component.

Optionally, the vertical moving part is set as an electric telescopic rod, and the fixed end of the electric telescopic rod is in transmission arrangement with the screw rod module.

Optionally, the magnetic attraction assembly comprises an electromagnetic column, a power supply and an on-off key, the electromagnetic column is electrically connected to the power supply and the on-off key, and the on-off key is fixedly arranged on the shell.

By adopting the technical scheme, the switch key is pressed to electrify and magnetize the electromagnetic column, so that magnetic bead liquid in gene sequencing reaction can be adsorbed, and then the transfer of sample DNA in gene sequencing reaction is realized.

Optionally, the shell is located the sample addition port is all opened and shut and is provided with the lid.

Through adopting above-mentioned technical scheme, cover and be favorable to improving the closure of card box.

Optionally, a waste liquid collecting tank is further arranged on the reagent tank and positioned in the shell.

Through adopting above-mentioned technical scheme, the waste liquid collecting vat can carry out centralized processing to the waste liquid of system storehouse in-process to be favorable to preventing chemical pollution.

In summary, the present application includes at least one of the following beneficial technical effects:

when a DNA sample needs to be subjected to gene sequencing and library building, each reaction solution is firstly added into each reagent groove of a reagent tray, and a shell is covered on the reagent tray. And putting the sample to be stored into the idle reagent groove of the reagent tray through the sample adding port. And finally, driving the magnetic suction assembly to perform gene sequencing library building operation among the reagent tanks through the moving assembly. The library after the library establishment can be taken out from a sampling port. The whole warehouse building operation is carried out in the shell, so that the possibility of sample pollution during warehouse building is reduced;

the multiple magnetic suction assemblies enable the card box to build the libraries of a plurality of groups of samples simultaneously, so that the library building efficiency of the plurality of groups of samples is improved;

the waste liquid collecting tank can be used for carrying out centralized treatment on waste liquid in the warehouse manufacturing process, so that chemical pollution can be prevented.

Drawings

FIG. 1 is a schematic diagram showing the overall structure of a closed cassette for gene sequencing library construction according to an embodiment of the present application;

FIG. 2 is a sectional view of the closed cartridge for gene sequencing and library construction according to the embodiment of the present application.

Reference numerals: 1. a housing; 2. a reagent tray; 3. a moving assembly; 31. the vertical moving member 31; 32. a lateral moving member 32; 4. a magnetic component; 5. a sample tank; 6. a magnetic bead liquid bath; 7. an ethanol tank; 8. a glycerol tank; 9. a primer probe groove; 10. a reaction liquid tank; 11. a buffer tank; 12. a water tank; 13. a library collection tank; 14. a waste liquid collecting tank; 15. a lofting opening; 16. a sampling port; 17. and (4) covering.

Detailed Description

The present application is described in further detail below with reference to figures 1-2.

The embodiment of the application discloses a closed card box for gene sequencing library construction.

Referring to fig. 1 and 2, a closed cassette for gene sequencing library construction includes a housing 1, a reagent tray 2, a moving assembly 3, and a magnetic attraction assembly 4.

The reagent tray 2 is rectangular and made of high temperature resistant material, and a plurality of reagent grooves are evenly arranged on the reagent tray 2, and the number of the reagent grooves is twenty two in the embodiment, and the twenty two reagent grooves are arranged according to a 2 x 11 array. In this embodiment, two reagent wells in the first row along the length direction of the reagent tray 2 are set as sample wells 5 for loading samples; the two reagent tanks in the second row are arranged as a magnetic bead liquid tank 6 for adding magnetic bead liquid; the third two reagent tanks are set as an ethanol tank 7 and are used for adding 75% ethanol; the two reagent tanks in the fourth row are set as a glycerol tank 8 for adding glycerol; the two reagent tanks in the fifth row are set as a primer probe tank 9 and are used for adding primer probe liquid for building a library; the reagent tanks in the sixth row and the seventh row are both arranged as reaction liquid tanks 10 for adding reaction liquid for building the library; the reagent tanks in the eighth row and the ninth row are arranged into a buffer solution tank 11 for adding buffer solution; the tenth row of reagent tanks is provided with a water tank 12 for adding cleaning water; the eleventh column of reagent wells is configured as a library collection well 13 for loading with eluent. Meanwhile, a directional waste liquid collecting tank 14 is arranged between the tenth row of water tanks 12 and the eleventh row of library collecting tank 13 and is used for collecting waste liquid generated after library preparation is finished.

The shell 1 is clamped on the upper plate of the reagent tray 2. The moving assembly 3 is located within the housing 1 and includes a vertically moving part 31 and a laterally moving part 32. The traverse member 32 is horizontally disposed in the housing 1, and the moving direction of the traverse member 32 is disposed along the longitudinal direction of the reagent tray 2. The transverse moving component 32 is provided with a screw rod module which is fixedly arranged on the inner side wall of the shell 1 and comprises a stepping motor, a screw rod and a sliding block. Vertical moving part 31 chooses for use to be electric telescopic handle, and electric telescopic handle is vertical to be set up downwards on the slider.

Subassembly 4 is inhaled to magnetism selects in this embodiment to be two, is located two lines of reagent groove tops respectively to can supply two sets of samples to build the storehouse simultaneously. Subassembly 4 is inhaled to magnetism includes electromagnetism post, power and on & off switch, and the vertical downward fixed setting of electromagnetism post is in electric telescopic handle's flexible end, and the electromagnetism post chooses for use for direct column iron core that is less than the reagent groove, and the one end winding that the column iron core is close to electric telescopic handle is provided with the wire, and the wire is connected with DC power supply electricity, and establishes ties with the on & off switch. The switch key is used for controlling whether the conducting wire is electrified or not, so that whether the electromagnetic column is magnetic or not is controlled.

Two sample adding ports 15 corresponding to the reagent grooves are arranged on the upper surface of the shell 1, and the sample adding ports 15 are positioned right above the sample grooves 5. The number of the sample adding ports 15 is two, and the shell 1 is provided with a cover 17 at the lofting port 15. The operator places the sample into the reagent reservoir through the sample port 15 before starting the library construction.

Two sampling ports 16 corresponding to the reagent tanks are arranged on the upper surface of the casing 1, and the sampling ports 16 are positioned right above the library collecting tank 13. The number of the sampling openings 16 is two, and the housing 1 is also provided with a cover 17 at the sampling openings 16. The operator can remove the library through the sampling port 16 after the library is completed.

The implementation principle of the closed card box for gene sequencing and library building in the embodiment of the application is as follows: the closed cartridge can be used for automatic library establishment of gene mutation detection kits such as ALK/BRAF/EGFR/PIKCA/KRAS and the like. The library construction operation is first performed, using a pipette to load the sample into the sample well 5. And adding matched reagents into the other reagent tanks, sealing the shell 1, and automatically preparing the library in the shell 1 by the moving assembly 3. And finally, library collection is carried out, a pipette is used for extending into the library collecting tank 13 from the sampling port 16, and the pipette is used for repeatedly blowing and beating for a plurality of times and then sucking into a centrifugal tube for subsequent quality control or sequencing operation.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. The utility model provides a gene sequencing builds storehouse and uses closed card box which characterized in that: the device comprises a reagent tray (2) and a shell (1), wherein the shell (1) is detachably arranged on the reagent tray (2), the reagent tray (2) is provided with a plurality of reagent grooves, and each reagent groove is positioned in the shell (1);

the shell (1) is provided with a sample adding port (15) for adding a sample to be built and a sampling port (16) for taking out a library;

still be provided with magnetism in casing (1) and inhale subassembly (4) and be used for removing magnetism inhales removal subassembly (3) of subassembly (4), magnetism is inhaled subassembly (4) transmission and is set up remove on subassembly (3).

2. The closed cassette for gene sequencing library building according to claim 1, wherein: the magnetic attraction component (4) is arranged in a plurality of ways, and the magnetic attraction component (4) is arranged on the moving component (3) in a transmission way.

3. The closed cassette for gene sequencing library building according to claim 1, wherein: the moving assembly (3) comprises a vertical moving component (31) and a transverse moving component (32), and the moving direction of the transverse moving component (32) is perpendicular to the moving direction of the vertical moving component (31).

4. The closed cassette for gene sequencing library building according to claim 3, wherein: the transverse moving component (32) is arranged as a screw rod module, the screw rod module is fixedly arranged on the inner side wall of the shell (1), and the transmission direction of the screw rod module is perpendicular to the moving direction of the vertical moving component (31).

5. The closed cassette for gene sequencing library building according to claim 4, wherein: vertical moving part (31) sets up to electric telescopic handle, electric telescopic handle's stiff end transmission set up in on the lead screw module.

6. The closed cassette for gene sequencing library building according to claim 1, wherein: the magnetic attraction component (4) comprises an electromagnetic column, a power supply and an on-off key, wherein the electromagnetic column is electrically connected with the power supply and the on-off key, and the on-off key is fixedly arranged on the shell (1).

7. The closed cassette for gene sequencing library building according to claim 1, wherein: the shell (1) is positioned at the sample adding port (15) and is provided with covers (17) in an opening and closing manner.

8. The closed cassette for gene sequencing library building according to claim 1, wherein: and a waste liquid collecting tank (14) is also arranged on the reagent tank and positioned in the shell (1).