CN215162773U - Full-automatic nucleic acid methylation amplification detection equipment - Google Patents
Full-automatic nucleic acid methylation amplification detection equipment Download PDFInfo
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- CN215162773U CN215162773U CN202023054938.1U CN202023054938U CN215162773U CN 215162773 U CN215162773 U CN 215162773U CN 202023054938 U CN202023054938 U CN 202023054938U CN 215162773 U CN215162773 U CN 215162773U
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The application discloses full-automatic nucleic acid methylation amplification detection equipment, which comprises a control mechanism, a pretreatment mechanism, a post-treatment mechanism, a transfer mechanism and detection, wherein the control mechanism is respectively communicated with the pretreatment mechanism, the post-treatment mechanism and the transfer mechanism; the pretreatment mechanism comprises a first magnetic rod sleeve, a first deep hole plate, a first oscillation assembly and a first magnetic attraction assembly, wherein the first deep hole plate is used for placing liquid to be treated; the first oscillating assembly is connected with the first deep hole plate, is positioned below the first deep hole plate and is used for oscillating the liquid in the first deep hole plate; the first magnetic suction assembly is used for preliminarily separating a detection sample to be treated in the liquid to be treated; the post-treatment mechanism comprises a second deep hole plate, a second oscillating assembly and a second magnetic attraction assembly, and realizes the sulfite conversion and purification of DNA.
Description
Technical Field
The application relates to the technical field of molecular diagnosis, in particular to a full-automatic nucleic acid methylation amplification detection device.
Background
DNA methylation is an important research context in Epigenetics (Epigenetics). Changes in DNA methylation status are an important factor in tumorigenesis, and include a decrease in global genomic methylation levels and an abnormally increased local methylation levels of CpG islands, resulting in genomic instability and thus the induction of cellular carcinogenesis.
The variations that occur at the epigenetic level are more frequent than the genetic mutations, and therefore the use of epigenetic variations to diagnose tumors has a higher sensitivity and better specificity than the detection at the level of genetic mutations. Because DNA methylation is an early event in tumorigenesis, early diagnosis of tumors by detecting the methylation level of genes has significant clinical utility.
Circulating tumor DNA (ctDNA) is a DNA fragment from the tumor genome carried in the human blood system. The main sources are necrotic tumor cells, apoptotic tumor cells, circulating tumor cells and exosomes secreted by tumor cells. These tumor DNAs often contain methylation characteristics that are characteristic of the tumor genome.
At present, manual methods are mostly adopted for purifying free DNA in plasma and treating sulfite, and even a plurality of kits can be used for realizing the purpose. The manual time is long, the steps are various and complex, the operating pressure of more than 32 samples is greatly increased, the stagnation time of the samples is different before and after the intermediate steps are various, and the repeatability of the detection of the samples is not high. One of the difficulties in the development of automated devices is the handling of large sample volumes, the volume of the intermediate treatment mixture can reach 10mL, and there is no corresponding consumable to match the automated instrument.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present application provides a fully automated nucleic acid methylation amplification detection apparatus.
The technical scheme of the application is as follows:
1. the full-automatic nucleic acid methylation amplification detection equipment is characterized by comprising a control mechanism, a pretreatment mechanism, a post-treatment mechanism, a transfer mechanism and a PCR mechanism, wherein the control mechanism is respectively communicated with the pretreatment mechanism, the post-treatment mechanism and the transfer mechanism;
the pretreatment mechanism comprises a first magnetic rod sleeve, a first deep hole plate, a first oscillation component and a first magnetic attraction component,
the first magnetic rod sleeve movably extends into the first deep hole plate, and the first magnetic suction assembly movably extends into the first magnetic rod sleeve;
the first deep hole plate is used for placing liquid to be treated;
the first oscillating assembly is connected with the first deep hole plate, is positioned below the first deep hole plate and is used for oscillating the liquid to be treated in the first deep hole plate;
the first magnetic suction assembly is used for preliminarily separating a detection sample to be treated in liquid to be treated;
the post-processing mechanism comprises a second deep hole plate, a second oscillating assembly and a second magnetic attraction assembly, wherein,
the second deep hole plate is used for placing the detection sample to be processed obtained by the pretreatment mechanism;
the second oscillating assembly is connected with the second deep hole plate and is used for oscillating the detection sample to be processed in the second deep hole plate;
the second magnetic suction assembly is used for separating a detection sample from a detection sample to be processed;
the PCR mechanism is used for amplifying, analyzing and detecting the detection sample obtained by the post-processing mechanism.
2. The apparatus for detecting nucleic acid methylation according to item 1, wherein the transfer mechanism is in communication with the first deep well plate and the second deep well plate, respectively, for transferring the detection sample to be processed in the first deep well plate into the second deep well plate.
3. The apparatus according to item 1, wherein the first magnetic assembly comprises a magnetic rod movably disposed above the first magnetic rod sleeve and movably extending into the first magnetic rod sleeve.
4. The full-automatic nucleic acid methylation amplification detection apparatus according to claim 3, wherein the pretreatment mechanism further comprises a second lifting assembly, and the second lifting assembly is connected with the magnetic rod and used for lifting the magnetic rod.
5. The fully automated nucleic acid methylation amplification detection apparatus according to item 1, wherein the sample is a sample;
the first deep well plate is one of a 24 deep well plate, a 32 deep well plate, a 48 deep well plate or a 96 deep well plate, and preferably, the first deep well plate is a 24 deep well plate or a 48 deep well plate;
the second deep well plate is one of a 24 deep well plate, a 32 deep well plate, a 48 deep well plate or a 96 deep well plate, and preferably, the second deep well plate is a 48 deep well plate.
6. The fully automatic nucleic acid methylation amplification detection apparatus according to claim 5, wherein the pretreatment mechanism further comprises a first lifting assembly and a first stage, the first lifting assembly is connected with the first stage, and the first lifting assembly is used for lifting the first stage;
the first magnetic rod sleeve is arranged on the first object stage.
7. The full-automatic nucleic acid methylation amplification detection apparatus according to item 1, wherein the pretreatment mechanism further comprises a first sample application component and a first transport component;
the first sample adding assembly is used for adding samples into the first deep hole plate;
the first transport assembly is used for transporting the first deep hole plate.
8. The apparatus according to item 5, wherein the second magnetic assembly comprises a magnetic frame, and the second deep well plate is movably disposed above the magnetic frame.
9. The fully automated nucleic acid methylation amplification detection apparatus according to item 1, wherein the post-processing mechanism further comprises a moving assembly and a second stage, the moving assembly is connected with the second stage, and the moving assembly is used for moving the second stage;
the second deep hole plate is arranged on the second objective table.
10. The fully automatic nucleic acid methylation amplification detection apparatus of item 5, wherein the post-processing mechanism further comprises a second sample application assembly, a second transport assembly, a heating assembly, and a pipetting assembly;
the second sample adding component is used for adding samples into the second deep hole plate;
the second transportation assembly is used for transporting the second deep hole plate or the waste liquid in the second deep hole plate;
the heating assembly is used for heating the detection sample to be processed in the second deep hole plate;
the pipetting assembly comprises a first pipetting assembly and a second pipetting assembly, and the first pipetting assembly is used for transferring the detection sample to be treated in the second deep hole plate; the second pipetting assembly is used for transferring the detection sample in the second deep hole plate into the PCR mechanism.
11. The full-automatic nucleic acid methylation amplification detection device according to item 1, wherein the liquid to be treated is plasma and lysate, the detection sample to be treated is DNA containing impurities, and the detection sample is DNA.
12. The apparatus according to claim 11, wherein in the pre-treatment mechanism, the plasma is lysed to obtain DNA, and the DNA is washed for the first time;
in the post-processing mechanism, the DNA containing impurities after the first washing is subjected to sulfite conversion, multiple washing and elution to obtain the DNA of the detection sample.
13. The apparatus for detecting nucleic acid methylation according to any one of claims 1 to 12, wherein the apparatus further comprises a display mechanism, the display mechanism is connected to the control mechanism, and is configured to give instructions to the control mechanism.
14. The apparatus for detecting full-automatic nucleic acid methylation amplification according to item 13, wherein the control mechanism is connected to the first lifting assembly, the first sample feeding assembly, the first transporting assembly, the first oscillating assembly, the second lifting assembly, the transferring mechanism, the moving assembly, the second sample feeding assembly, the second transporting assembly, the second oscillating assembly, the pipetting assembly, the heating assembly, and the PCR mechanism, respectively.
According to the full-automatic nucleic acid methylation amplification detection equipment, DNA in blood plasma is cracked through the pretreatment mechanism, then the cracked DNA is washed for the first time through the transfer mechanism, then the washed liquid is transferred to the post-treatment mechanism, and the post-treatment mechanism carries out operations such as sulfite conversion, multiple washing, purification and the like on the DNA obtained through pretreatment, so that the target DNA is finally obtained. Moreover, the plasma volume of a single sample processed by the device at one time can reach 3.5ml, which is far beyond the once processing volume of the nucleic acid processing device in the prior art, so that the high-throughput processing of the single plasma sample from a plurality of different sources can be realized, and the high-throughput detection can be realized.
Drawings
The drawings are included to provide a further understanding of the application and are not to be construed as limiting the application. Wherein:
FIG. 1 is a schematic diagram of the structure of a fully automated nucleic acid methylation amplification detection apparatus according to the present application.
Detailed Description
The following description of the exemplary embodiments of the present application, taken in conjunction with the accompanying drawings, includes various details of the embodiments of the application for the understanding of the same, which are to be considered exemplary only. Accordingly, those of ordinary skill in the art will recognize that various changes and modifications of the embodiments described herein can be made without departing from the scope and spirit of the present application. Also, descriptions of well-known functions and constructions are omitted in the following description for clarity and conciseness.
As shown in fig. 1, the application discloses a full-automatic nucleic acid methylation amplification detection device, which comprises a control mechanism, a pretreatment mechanism, a post-treatment mechanism, a transfer mechanism and a PCR mechanism, wherein the control mechanism is respectively communicated with the pretreatment mechanism, the post-treatment mechanism and the transfer mechanism, and the pretreatment mechanism is communicated with the post-treatment mechanism through the transfer mechanism; the pre-treatment mechanism and the post-treatment mechanism are controlled by the control mechanism.
The pretreatment mechanism comprises a first magnetic rod sleeve, a first deep hole plate, a first oscillation component and a first magnetic attraction component,
the first magnetic rod sleeve movably extends into the first deep hole plate, and the first magnetic suction assembly movably extends into the first magnetic rod sleeve;
the first deep hole plate is used for placing liquid to be processed (the liquid to be processed is blood plasma and lysate);
the first magnetic rod sleeve extends into the first deep hole plate to move up and down, so that liquid to be treated in the first deep hole plate is beaten up and down to accelerate cracking of blood plasma.
The first oscillating assembly is connected with the first deep hole plate, is positioned below the first deep hole plate and is used for oscillating the plasma and the lysate which are arranged in the first deep hole plate, so that the plasma and the lysate are uniformly mixed and are rapidly cracked;
the first magnetic suction assembly is used for preliminarily separating a detection sample to be processed in liquid to be processed, namely, when the device is used, the liquid to be processed and magnetic beads are added into the first deep hole plate simultaneously. After the plasma in the first deep hole plate is cracked to obtain DNA, the DNA containing impurities is adsorbed on magnetic beads, the first magnetic attraction component extends into the first magnetic rod sleeve, the magnetic beads carrying the DNA in the first deep hole plate are adsorbed on the outer wall of the first magnetic rod sleeve due to the magnetic attraction effect, then the first deep hole plate is moved away, a new first deep hole plate is placed below the first magnetic rod sleeve, then the first magnetic attraction component is moved away, and the magnetic beads carrying the DNA can fall into the new first deep hole plate, so that the DNA containing impurities is obtained;
the post-processing mechanism comprises a second deep hole plate, a second oscillating assembly and a second magnetic attraction assembly, wherein,
the second deep hole plate is used for placing a detection sample (DNA solution containing impurities) to be treated obtained by the pretreatment mechanism;
the second oscillating assembly is connected with the second deep hole plate and is used for oscillating the DNA solution containing the impurities in the second deep hole plate so as to wash and elute the DNA solution containing the impurities for multiple times;
the second magnetic attraction component is used for separating a detection sample from the detection sample to be processed.
The second magnetic attraction component is positioned below the second deep hole plate, adopts a lower magnetic attraction mode, can be matched with the second oscillation component to realize the full oscillation of the mixed liquid, and is matched with the liquid transfer component to remove trace residual liquid;
the PCR mechanism is used for amplifying, analyzing and detecting the detection sample obtained by the post-processing mechanism.
The PCR mechanism is a conventional PCR instrument and is used for amplification, fluorescence detection and the like.
In the present application, the first deep well plate is one of a 24-deep well plate, a 32-deep well plate, a 48-deep well plate or a 96-deep well plate, and preferably, the first deep well plate is a 24-deep well plate or a 48-deep well plate;
the second deep well plate is one of a 24 deep well plate, a 32 deep well plate, a 48 deep well plate or a 96 deep well plate, and preferably, the second deep well plate is a 48 deep well plate.
When the first deep well plate is a 48 deep well plate and the second deep well plate is a 48 deep well plate, the first deep well plate can process 3.5ml of plasma sample per well, and the first deep well plate can process up to 48 different plasma samples.
In the pretreatment mechanism, plasma and lysate are mixed, DNA is cracked from the plasma through oscillation of the first oscillation assembly and up-and-down movement of the first magnetic rod sleeve, then the DNA is subjected to primary separation through the first magnetic suction assembly, and is subjected to primary washing in the new first deep hole plate, so that an impurity-containing DNA solution is obtained.
In the post-processing mechanism, the DNA solution containing impurities is subjected to sulfite conversion, washing a plurality of times and elution by oscillation of the second oscillating module, thereby obtaining a detection sample.
The first oscillating assembly includes an oscillator by which vortex oscillation is achieved.
And the second oscillating component comprises an oscillator by means of which horizontal oscillation can be achieved.
The control mechanism comprises a computer, and signal transmission and control of mechanical parts can be realized through a VGA connecting wire.
The transfer mechanism may specifically be a robotic arm, which may be connected to a gripper arm or a pipetting arm.
In this application, first magnetism is inhaled the subassembly and is included the magnetic rod, the magnetic rod movably set up in the top of first magnetic rod sleeve pipe to movably stretch into in the first magnetic rod sleeve pipe, through the effect of magnetism, the magnetic bead that will carry DNA adsorbs first magnetic rod sheathed tube outer wall to carry out primary separation with DNA.
The use principle of the pretreatment mechanism is as follows:
adding plasma, lysate and magnetic beads into the first deep hole plate, driving the first magnetic rod sleeve to move up and down through the first lifting assembly and the oscillation of the first vibration assembly, so that the mixed liquid in the first deep hole plate oscillates uniformly, the plasma cracks DNA, when the DNA is cracked from the plasma, the magnetic rod extends into the first magnetic rod sleeve, the DNA containing impurities is enriched on the magnetic beads, the magnetic rod extends into the first magnetic rod sleeve, the magnetic beads are adsorbed on the outer wall of the first magnetic rod sleeve under the action of magnetic force, then the first deep hole plate with waste liquid is removed, a new first hole plate added with washing liquid is placed below the first magnetic rod sleeve, then the magnetic rod is removed, and the magnetic beads fall into the new first deep hole plate from the outside of the first magnetic rod sleeve, the DNA containing impurities on the magnetic beads enters a washing solution, and the DNA containing impurities is washed for the first time through oscillation of the first oscillating assembly and up-and-down movement (also called up-and-down sucking) of the first magnetic rod sleeve.
The number of the magnetic rods is the same as that of the first magnetic rod sleeves and the first deep hole plate.
The magnetic beads are small nano-scale iron beads, and are present in a liquid form, the concentration of the magnetic beads is 40mg/ml, and when the magnetic beads are added, for example, 90 μ l of magnetic bead solution can be added to each well, that is, 3.6mg of magnetic beads are added to each well, although the content of the magnetic beads can be adjusted according to different samples. Magnetic beads are available from ZinextsLife Science Corp Chuanmaosheng technology, Inc.
The magnetic beads in the pre-treatment mechanism and the post-treatment mechanism may be the same.
In the application, the transfer mechanism is respectively communicated with the first deep hole plate and the second deep hole plate and is used for transferring the liquid in the first deep hole plate into the second deep hole plate.
After the first washing, the liquid in the first deep well plate and the magnetic beads are transferred to the second deep well plate by the transfer mechanism.
In this application, pretreatment mechanism still includes second lifting unit, second lifting unit with the magnetic rod is connected (the electricity is connected), is used for going up and down the magnetic rod.
The second lifting assembly may be a hydraulic lifting device or a pneumatic lifting device.
The second lifting assembly can drive the magnetic rod to stretch into the first magnetic rod sleeve so as to adsorb magnetic beads.
In the application, the pretreatment mechanism further comprises a first lifting assembly and a first object stage, wherein the first lifting assembly is connected with the first object stage and is used for lifting the first object stage; the first magnetic rod sleeve is arranged on the first object stage.
The first lifting assembly can drive the first object stage and the first magnetic rod sleeve to lift, when first washing is carried out, the first magnetic rod sleeve is lifted to a certain height through the first lifting assembly, then the first deep hole plate is moved away, a new first deep hole plate added with washing liquor is placed under the first magnetic rod sleeve, and then the first magnetic rod sleeve is lowered into the new first deep hole plate through the first lifting assembly to carry out first washing.
The first lifting assembly may be a hydraulic lifting device or a pneumatic lifting device.
In the application, the pretreatment mechanism further comprises a first sample adding component and a first transporting component,
the first sample adding assembly is used for adding samples into the first deep hole plate, and blood plasma, lysate or washing liquor can be added into the first deep hole plate through the first sample adding assembly;
the first transportation assembly is used for transporting the first deep hole plate (before the first washing, the first deep hole plate containing waste liquid is transported away by the first transportation assembly, and a new first deep hole plate is transported over).
First application of sample subassembly includes pipetting tip and cooperation pipetting tip's single channel, binary channels, 6 passageway or 8 passageway application of sample subassemblies.
The first transportation assembly comprises an X-axis guide rail, a Y-axis guide rail or a Z-axis guide rail and is used for moving up and down, back and forth, left and right, and the transportation function is realized.
In this application, the second subassembly of inhaling is inhaled to magnetism includes the magnetic force frame, the second deep hole board movably sets up in the upper portion of magnetic force frame.
In this application, the post-processing mechanism further comprises a moving assembly and a second stage, the moving assembly is connected with the second stage, and the moving assembly is used for moving the second stage; the second deep hole plate is arranged on the second objective table.
The post-processing mechanism also comprises a second sample adding assembly, a second transportation assembly, a heating assembly and a liquid transferring assembly;
the second sample adding component is used for adding samples into the second deep hole plate;
the second transportation assembly is used for transporting the second deep hole plate or the waste liquid in the second deep hole plate;
the heating assembly is used for heating the DNA solution in the second deep hole plate.
The second application of sample subassembly includes pipetting tip and cooperation pipetting tip's single channel, binary channels, 6 passageways or 8 passageways pipetting subassembly.
The second transportation assembly comprises an X-axis guide rail, a Y-axis guide rail or a Z-axis guide rail and is used for moving front, back, left and right or up and down to realize the transportation function.
The heating assembly may be a pure metal bath, a metal module gas bath or a water bath heating assembly.
The pipetting assembly comprises a first pipetting assembly and a second pipetting assembly, and the first pipetting assembly is used for transferring the detection sample to be treated in the second deep well plate into a new second deep well plate in the elution process; the second pipetting assembly is used for transferring the detection sample in the second deep hole plate into the PCR mechanism.
First move liquid subassembly and second move liquid subassembly all can include move liquid suction head and with move liquid suction head matched with single channel, binary channels or 6 passageway, 8 passageway subassemblies.
The use principle of the post-processing mechanism is as follows:
after the first washing, the mixed liquor entering the second deep hole plate is oscillated and uniformly mixed through the second oscillating assembly, DNA is enriched on the magnetic beads, then the second deep hole plate is moved to the magnetic frame, and the magnetic beads with the DNA are adsorbed on the side face of the bottom of the second deep hole plate through the magnetic attraction effect. Then removing the liquid in the second deep hole plate through a second transportation assembly, and only keeping the magnetic beads; then the second application of sample subassembly adds the eluant to the second deep hole board, then will through moving the subassembly the second deep hole board is followed the magnetic force frame is gone up and is removed, later opens the second and shakes the subassembly and carry out the oscillation mixing after 10 minutes, passes through again the removal subassembly moves the second deep hole board to the magnetic force frame on, adsorbs the magnetic bead in second deep hole board bottom side through the magnetic attraction effect, then passes through the second transport subassembly transports new second deep hole board to appointed position, shifts the eluant that contains DNA in the second deep hole board to new second deep hole board through first transfer liquid subassembly, second transport subassembly transports old second deep hole board away to accomplish elution for the first time.
And after the first elution, adding a sulfite solution into the new second deep hole plate, uniformly mixing the sulfite solution and the DNA through the second oscillating assembly, heating to 80 ℃ through the heating assembly, and maintaining for 45min for methylation reaction to obtain methylated DNA. Then adding washing liquor and magnetic beads into the second deep-hole plate, and carrying out oscillation and uniform mixing treatment; and then the second deep hole plate is transferred to the magnetic frame through the moving assembly, and after the waste liquid removing treatment is completed twice through the second transportation assembly, the second deep hole plate is removed from the magnetic frame through the moving assembly. And adding washing liquid into the second deep hole plate again, and performing oscillation mixing and magnetic absorption to remove waste liquid to finish secondary washing.
After the second washing, standing the second deep hole plate for a preset time period, adding an eluent, sequentially carrying out oscillation and uniform mixing and magnetic absorption treatment, transferring the product, and finishing the extraction.
And when the post-processing mechanism finishes extraction, transferring the DNA in the second deep hole plate into the PCR mechanism through the second pipetting assembly for PCR amplification, detection and analysis.
In this application, the device further comprises a display mechanism, the display mechanism is connected with the control mechanism and used for giving instructions to the control mechanism. The control mechanism is respectively connected with the first lifting assembly, the first sample adding assembly, the first conveying assembly, the first oscillating assembly, the second lifting assembly, the transferring mechanism, the moving assembly, the second sample adding assembly, the second conveying assembly, the second oscillating assembly, the liquid transferring assembly and the heating assembly. Through control mechanism, display mechanism can control first lifting unit, first application of sample subassembly, first transportation subassembly, first vibration subassembly, second lifting unit, transfer mechanism, removal subassembly, second application of sample subassembly, second transportation subassembly, second vibration subassembly, move liquid subassembly, heating element and PCR mechanism.
Although the embodiments of the present application have been described above with reference to the accompanying drawings, the present application is not limited to the above-described embodiments and application fields, and the above-described embodiments are illustrative, instructive, and not restrictive. Those skilled in the art, having the benefit of this disclosure, may effect numerous modifications thereto and changes may be made without departing from the scope of the invention as defined by the appended claims.
Claims (11)
1. The full-automatic nucleic acid methylation amplification detection equipment is characterized by comprising a control mechanism, a pretreatment mechanism, a post-treatment mechanism, a transfer mechanism and a PCR mechanism, wherein the control mechanism is respectively communicated with the pretreatment mechanism, the post-treatment mechanism and the transfer mechanism;
the pretreatment mechanism comprises a first magnetic rod sleeve, a first deep hole plate, a first oscillation component and a first magnetic attraction component,
the first magnetic rod sleeve movably extends into the first deep hole plate, and the first magnetic suction assembly movably extends into the first magnetic rod sleeve;
the first deep hole plate is used for placing liquid to be treated;
the first oscillating assembly is connected with the first deep hole plate, is positioned below the first deep hole plate and is used for oscillating the liquid to be treated in the first deep hole plate;
the first magnetic suction assembly is used for preliminarily separating a detection sample to be treated in liquid to be treated;
the post-processing mechanism comprises a second deep hole plate, a second oscillating assembly and a second magnetic attraction assembly, wherein,
the second deep hole plate is used for placing the detection sample to be processed obtained by the pretreatment mechanism;
the second oscillating assembly is connected with the second deep hole plate and is used for oscillating the detection sample to be processed in the second deep hole plate;
the second magnetic suction assembly is used for separating a detection sample from a detection sample to be processed;
the PCR mechanism is used for amplifying, analyzing and detecting the detection sample obtained by the post-processing mechanism.
2. The fully automated nucleic acid methylation amplification detection apparatus according to claim 1, wherein the transfer mechanism is respectively in communication with the first deep well plate and the second deep well plate, and is configured to transfer the detection sample to be processed in the first deep well plate into the second deep well plate.
3. The fully automated nucleic acid methylation amplification detection apparatus of claim 1, wherein the first magnetic assembly comprises a magnetic rod movably disposed above the first magnetic rod sleeve and movably extending into the first magnetic rod sleeve.
4. The fully automatic nucleic acid methylation amplification detection apparatus according to claim 3, wherein the pre-processing mechanism further comprises a second lifting assembly, and the second lifting assembly is connected with the magnetic rod and used for lifting the magnetic rod.
5. The fully automated nucleic acid methylation amplification detection apparatus of claim 4, wherein;
the first deep well plate is one of a 24 deep well plate, a 32 deep well plate, a 48 deep well plate or a 96 deep well plate;
the second deep well plate is one of a 24 deep well plate, a 32 deep well plate, a 48 deep well plate or a 96 deep well plate.
6. The fully automated nucleic acid methylation amplification detection apparatus of claim 4, wherein;
the first deep hole plate is a 24 deep hole plate or a 48 deep hole plate;
the second deep hole plate is a 48 deep hole plate.
7. The fully automated nucleic acid methylation amplification detection apparatus according to claim 5 or 6, wherein the pre-processing mechanism further comprises a first lifting assembly and a first stage, the first lifting assembly is connected with the first stage, and the first lifting assembly is used for lifting the first stage;
the first magnetic rod sleeve is arranged on the first object stage;
the pretreatment mechanism further comprises a first sample adding assembly and a first transportation assembly;
the first sample adding assembly is used for adding samples into the first deep hole plate;
the first transport assembly is used for transporting the first deep hole plate.
8. The fully automated nucleic acid methylation amplification detection apparatus of claim 7, wherein the second magnetic assembly comprises a magnetic rack, and the second deep well plate is movably disposed above the magnetic rack;
the post-processing mechanism further comprises a moving assembly and a second object stage, the moving assembly is connected with the second object stage, and the moving assembly is used for moving the second object stage;
the second deep hole plate is arranged on the second objective table.
9. The fully automated nucleic acid methylation amplification detection apparatus of claim 8, wherein the post-processing mechanism further comprises a second sample application assembly, a second transport assembly, a heating assembly, and a pipetting assembly;
the second sample adding component is used for adding samples into the second deep hole plate;
the second transportation assembly is used for transporting the second deep hole plate or the waste liquid in the second deep hole plate;
the heating assembly is used for heating the detection sample to be processed in the second deep hole plate;
the pipetting assembly comprises a first pipetting assembly and a second pipetting assembly, and the first pipetting assembly is used for transferring the detection sample to be treated in the second deep hole plate; the second pipetting assembly is used for transferring the detection sample in the second deep hole plate into the PCR mechanism.
10. The fully automated nucleic acid methylation amplification detection apparatus of claim 9, further comprising a display mechanism coupled to the control mechanism for commanding the control mechanism.
11. The apparatus for detecting nucleic acid methylation according to claim 10, wherein the control mechanism is connected to the first lifting assembly, the first sample feeding assembly, the first transporting assembly, the first oscillating assembly, the second lifting assembly, the transferring mechanism, the moving assembly, the second sample feeding assembly, the second transporting assembly, the second oscillating assembly, the pipetting assembly, the heating assembly, and the PCR mechanism.
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| CN116410842A (en) * | 2021-12-31 | 2023-07-11 | 博尔诚（北京）科技有限公司 | Full-automatic nucleic acid methylation treatment equipment and treatment mechanism |
| CN115353953B (en) * | 2022-09-23 | 2024-04-16 | 博尔诚（北京）科技有限公司 | Shelter for health examination |
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| CN105543089A (en) * | 2016-03-08 | 2016-05-04 | 湖南圣湘生物科技有限公司 | Magnetic bead based nucleic acid extraction device and method |
| CN208964916U (en) * | 2018-06-26 | 2019-06-11 | 嘉兴医脉赛科技有限公司 | A kind of magnetic beads for purifying instrument of enrichment method |
| CN109022417A (en) * | 2018-08-13 | 2018-12-18 | 益善生物技术股份有限公司 | A kind of paramagnetic particle method nucleic acid extraction conversion reagent box and its application method |
| CN110511865A (en) * | 2019-08-29 | 2019-11-29 | 中国科学院苏州生物医学工程技术研究所 | High-throughput nucleic acid extraction system |
| CN215162773U (en) * | 2019-12-16 | 2021-12-14 | 博尔诚（北京）科技有限公司 | Full-automatic nucleic acid methylation amplification detection equipment |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608829A (en) * | 2019-12-16 | 2021-04-06 | 博尔诚（北京）科技有限公司 | Full-automatic nucleic acid methylation amplification detection equipment |
| CN112608829B (en) * | 2019-12-16 | 2023-01-31 | 博尔诚（北京）科技有限公司 | Full-automatic nucleic acid methylation amplification detection equipment |
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| CN112608830A (en) | 2021-04-06 |
| CN112608829A (en) | 2021-04-06 |
| CN215103283U (en) | 2021-12-10 |
| CN112608829B (en) | 2023-01-31 |
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