CN207699585U - Whole Blood Genomic DNA automates extraction system - Google Patents
Whole Blood Genomic DNA automates extraction system Download PDFInfo
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- CN207699585U CN207699585U CN201721337130.XU CN201721337130U CN207699585U CN 207699585 U CN207699585 U CN 207699585U CN 201721337130 U CN201721337130 U CN 201721337130U CN 207699585 U CN207699585 U CN 207699585U
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- genomic dna
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Abstract
The utility model discloses a kind of Whole Blood Genomic DNAs to automate extraction system, including shell, processing unit and control unit.Processing unit and control unit are installed in shell, and processing unit includes sample process platform and mechanical arm, and guide rail is equipped in shell, and the both ends of guide rail and the inner end wall of shell connect.Mechanical arm is slidably mounted on guide rail, control unit and mechanical arm signal communication and control machinery arm moves on guide rail, sample process platform include bottom plate and the suction nozzle box carrier, reagent trough carrier, centrifuge tube carrier, PCR plate carrier, sample tube carrier, fixed loading shelf and the waste holder carrier that are sequentially arranged on bottom plate.The utility model can greatly reduce the loss of cost and improve working efficiency, after a flux maximum can carry out 96 tests, and machine is run in extraction system completely without human intervention.
Description
Technical field
The utility model is related to nucleic acid extraction fields, and in particular to a kind of Whole Blood Genomic DNA automation extraction system.
Background technology
Paramagnetic particle method nucleic acid extraction kit is the new high-tech product of both bioscience and nanometer material science unification.
According to principle identical with pellosil centrifugal column, improvement and table are carried out to the surface of superparamagnetic nano particle with nanotechnology
After the modification of face, it is prepared into superparamagnetism silica nanometer magnetic bead.The magnetic bead can on micro interface with nucleic acid molecules specifically
Identification and efficiently combination.Using the superparamagnetism of silica nanoparticle, in Chaotropic salt (guanidine hydrochloride, guanidinium isothiocyanate
Deng) and externally-applied magnetic field under the action of, can from blood, animal tissue, food, pathogenic microorganism equal samples DNA and RNA separation
Out, it can be applicable to clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detection, food safety detection, divide
The multiple fields such as sub- biological study.
Paramagnetic particle method nucleic acid extraction can be generally divided into four steps：Sample cracking release nucleic acid, magnetic bead specific adsorption are combined and are waited for
DNA, washing purifying magnetic bead-DNA mixtures are detached, magnetic bead is detached with the elution of DNA to be separated.I.e. after cell cracking, to cracking
Magnetic bead is added in liquid, when the pH value of solution is less than 6.5, being combined with the DNA of optimization for magnetic bead selectivity will be adsorbed at this time
The magnetic bead of DNA is placed in magnetic field, removes the impurity (albumen etc.) not adsorbed by buffer solution, magnetic bead is then placed in pH value
In 8.5 buffer solution, the DNA of purifying can enter in buffer solution.
With the high speed development of molecular biology in recent years, large quantities of molecular biology based on nucleic acid have been emerged
Experiment, such as the sequencing of two generations, quantitative fluorescent PCR, genetic chip, making nucleic acid molecular hybridization, and the integrality of DNA, RNA degrade
Degree, the purity of nucleic acid and concentration etc. all generate molecular biology experiment more or less influence.Therefore molecular biology is real
Problems faced first is tested to be how quick, efficient, high quality to extract required nucleic acid from complicated and diversified sample.
With the fast development of medicine and scientific research cause, the sample of nucleic acid extraction is in quantity cumulative year after year and complexity at present
The trend deepened is spent, therefore in order to adapt to the trend of nucleic acid extraction, occurs the nucleic acid extraction of a collection of high throughput currently on the market
Instrument is broadly divided into three classes according to the difference of extraction principle：Silicon dielectric posts method, suction method and bar magnet method.
First method：Silicon dielectric posts method
The main body of instrument is centrifuge (vacuum pump), mechanical arm, centrifugal column, is sequentially completed cracking, combination, washing and elution
Process.This scheme the degree of automation is minimum in three kinds of schemes, is relatively suitble to sample size relatively low, thin, not sticky
Sample, such as a small amount of bacterium solution, blood have uncracked fill if sample size is bigger during nucleic acid burst size is bigger or sample
The sample divided is easy to the problem of blocking up pillar occur in centrifugal process.And sample needs are filled with liquid nitrogen or beveller in advance
Divide grinding, then multiple centrifugation steps, program are relatively complicated.
Second method：Suction method
The method is large-scale liquor removing workstation by the way of, and apparatus subject includes the automation volley of rifle fire, magnetic sheet etc., utilizes magnetic
Pearl completes the absorption to nucleic acid, and then the volley of rifle fire shifts waste liquid, and for the sample of different viscosity properties, can be configured,
Ensure the accuracy of liquid relief, and only need to think to be put into required reagent consumptive material, waits for end to come to take out production after running program
Object can be accomplished to automate completely, and automation mechanized operation ensure that experimental result is stablized, and avoid difference caused by manual operation
And mistake.Intellectualized operation system pollutes between stringent control hole hole, extracts casing using disposable plastic, avoids difference
It is polluted between batch.
The third scheme：Bar magnet method
Apparatus subject and consumptive material include bar magnet, stirring set, heating refrigeration module and deep-well plates etc., are carrying out nucleic acid extraction
When, sample is added in deep-well plates and is cracked, while containing magnetic bead in system, it can be by the nucleic acid absorption in lysate to magnetic
Bead surface, miscellaneous process of washing hereafter are completed by magnetic bead of the bar magnet transfer with nucleic acid in different deep-well plates, and this scheme carries
The nucleic acid salinity that takes remains low, purity higher, and the case where be not in stifled pillar and pipette tips, but needs in operation
Manual intervention is carried out, can not accomplish to automate completely.
Different method for extracting nucleic acid compare referring to following table one：
Table one
Prior art major defect is the similar problems such as the degree of automation is relatively low, working efficiency is low.
Utility model content
The purpose of this utility model is to provide a kind of Whole Blood Genomic DNA automation extraction system, above-mentioned existing to solve
The problem of technology.
To solve the above-mentioned problems, the utility model provides a kind of Whole Blood Genomic DNA automation extraction system, packet
Shell, processing unit and control unit are included, the processing unit and described control unit are installed in the shell, the processing
Unit includes sample process platform and mechanical arm, is equipped with guide rail in the shell, the both ends of the guide rail are interior with the shell
End wall connects, and the mechanical arm is slidably mounted on the guide rail, described control unit and the mechanical arm signal communication
And control the mechanical arm and moved on the guide rail, the sample process platform includes bottom plate and is sequentially arranged at the bottom plate
On suction nozzle box carrier, reagent trough carrier, centrifuge tube carrier, PCR plate carrier, sample tube carrier, fixed loading shelf and waste holder
Carrier.
Preferably, the guide rail is located at the surface of the sample process platform.
Preferably, distance of the guide rail apart from the sample process platform is 4cm-17cm.
Preferably, the suction nozzle box carrier includes chamber, it is equipped with support element in the chamber, the locating piece is from the chamber
The integral middle of room stretches out upwards.
Preferably, the support element is two circular abutments stretched out upwards from the bottom of the chamber.
Preferably, the distance between two circular abutments is 4cm.
Preferably, the suction nozzle box carrier includes 4 chambers, the suction of 96 loading amount of different size is placed in each chamber
Head cartridge.
Preferably, the reagent trough carrier includes elongated bottom, it is equipped on the bottom of the strip upward
The more root posts stretched out often define a kit screens between adjacent two root posts.
Preferably, the end of the reagent trough carrier is equipped with handle.
Preferably, the handle is from end horizontal stretching and reclinate claw.
Preferably, the centrifuge tube carrier includes carrier main body and handle, the carrier main body is centrifuged equipped with multiple placements
The hole of pipe and the handle are integrally stretched out from the end of the carrier main body to be formed.
Preferably, the PCR plate carrier is equipped with multiple screens, each screens is for placing a PCR plate.
Preferably, the centrifuge tube carrier includes centrifuge tube carrier main body and handle, the handle of the centrifuge tube carrier from
The end of the centrifuge tube carrier main body, which is integrally stretched out, to be formed.
Preferably, the fixed loading shelf is fixedly installed on the bottom plate of the sample process platform, and it include concussion heating
Module, transfer stand module and magnetic board magnetically attractive module.
Preferably, the waste holder carrier is movably installed on the bottom plate of the sample process platform, and include more
A chamber for being used for being respectively intended to abandon useless suction nozzle and waste liquid (separation of solid and liquid).
The Whole Blood Genomic DNA automatic extracting system of the utility model can greatly reduce the loss of cost and improve work
Make efficiency, after a flux maximum can carry out 96 tests, and machine is run in extraction system completely without human intervention.
Description of the drawings
Fig. 1 is the Whole Blood Genomic DNA automation extraction system schematic diagram of the utility model.
Fig. 2 is the schematic diagram of the sample process platform of the utility model.
Fig. 3 is the schematic diagram of the suction nozzle box carrier of the utility model.
Fig. 4 is the schematic diagram of the reagent trough carrier of the utility model.
Fig. 5 is the schematic diagram of the centrifuge tube carrier of the utility model.
Fig. 6 is the schematic diagram of the PCR plate carrier of the utility model.
Fig. 7 is the schematic diagram of the sample tube carrier of the utility model.
Fig. 8 is the schematic diagram of the waste holder carrier of the utility model.
Specific implementation mode
The preferred embodiment of the utility model is described in detail below with reference to attached drawing, understands this reality to become apparent from
With novel objects, features and advantages.It should be understood that embodiment shown in the drawings is not the limit to the scope of the utility model
System, and simply to illustrate that the connotation of technical solutions of the utility model.
It is described in detail referring to the Whole Blood Genomic DNA automation extraction system of Fig. 1-8 pairs of the utility model.Its
In, Fig. 1 is the Whole Blood Genomic DNA automation extraction system schematic diagram of the utility model, and Fig. 2 is at the sample of the utility model
The schematic diagram of platform, Fig. 3 are the schematic diagrames of the suction nozzle box carrier of the utility model, and Fig. 4 is that the reagent trough of the utility model carries
The schematic diagram of frame, Fig. 5 are the schematic diagrames of the centrifuge tube carrier of the utility model, and Fig. 6 is showing for the PCR plate carrier of the utility model
It is intended to, Fig. 7 is the schematic diagram of the sample tube carrier of the utility model, and Fig. 8 is the schematic diagram of the waste holder carrier of the utility model.
As shown in Figure 1, Whole Blood Genomic DNA automation extraction system 100 includes that shell 10, processing unit 20 and control are single
Member 30.Processing unit 20 and control unit 30 are installed in shell 10.Wherein, control unit 30 can be installed on appointing in shell
It anticipates suitable position, and control unit 30 further includes touch screen computer (not shown), it can be to control by touch screen computer
Unit 30 processed is operated.
Processing unit 20 includes sample process platform 21 and mechanical arm 22, is equipped with guide rail 11 in shell 10, and the two of guide rail 11
End is connect with the inner end wall of shell 10, and mechanical arm 22 is slidably mounted on guide rail 11, control unit 30 and mechanical arm signal
It is connected to and control machinery arm 22 moves on guide rail.Since control unit 30 includes touch screen computer, it need to only touch
It touches and carries out corresponding operating on screen, so that it may with the movement of operating robotic arm.Preferably, guide rail 11 is being located at sample process platform 21 just
Top, and the distance of 11 distance sample processing platform 21 of guide rail is 14cm, the setting of this distance can facilitate standby mode flat
The suitable displacement distance of the handling of all modules and all modules of working condition platform on platform.
As shown in Fig. 2, sample process platform 21 includes bottom plate 210 and the suction nozzle box carrier being sequentially arranged on bottom plate 210
211, reagent trough carrier 212, centrifuge tube carrier 213, PCR plate carrier 214, sample tube carrier 215, fixed loading shelf 216 and useless
Object box carrier 217.
As shown in figure 3, suction nozzle box carrier 211 is removably installed on bottom plate 210 and includes chamber 2111, chamber 2111
Interior to be equipped with support element 2112, support element 2112 stretches out upwards from the bottom of chamber 2111 one to be formed, preferably, support element 2112
Distance for two circular abutments stretched out upwards from the bottom of chamber 2111, two circular abutments is 4cm.It is shown in Fig. 2
Example in, suction nozzle box carrier 211 includes 4 chambers 2111, and the suction of 96 loading amount of different size is placed in each chamber 2111
Head cartridge.
As shown in figure 4, reagent trough carrier 212 is removably installed in close to suction nozzle box carrier 211 on bottom plate 210, reagent
Slot carrier 212 includes elongated bottom 2121, and the more root posts stretched out upwards are equipped on the bottom of strip 2121
2122, often define that a kit screens 2123, each (not shown) are installed on each examination between adjacent two root posts
In agent box screens 2123.It is equipped with handle 2124 in the end of reagent trough carrier, is taken reagent trough carrier 212 from bottom plate with facilitating
Down or it is installed on bottom plate.State in the example presented in the figure, handle 212 be from end horizontal stretch out and it is reclinate hook-shaped
Part.
As shown in figure 5, centrifuge tube carrier 213 is installed close to reagent trough carrier 212 and includes carrier main body 2131 and handle
2132, carrier main body 2131 is equipped with multiple holes 2133 for placing centrifuge tube, left end of the handle 2132 from carrier main body 213
Portion, which is integrally stretched out, to be formed, and positioned at the top of carrier main body 2131.
As shown in fig. 6, PCR plate carrier is installed on bottom plate 210 close to centrifuge tube carrier 213 and is equipped with 4 screens, often
One screens is for placing a PCR plate, it is of course possible to understand, PCR plate carrier can also be arranged extra 4 or less than 4
Screens.
As shown in fig. 7, sample tube carrier 215 is removably installed in close to PCR plate carrier on bottom plate 210, sample tube carries
Frame 215 includes sample tube carrier main body 2150 and handle 2151, and sample tube carrier handle 2151 is from sample tube carrier main body 2150
End integrally stretch out formed and positioned at sample tube carrier main body top.Centrifuge tube is can be seen that by comparison diagram 5 and Fig. 7
For carrier 213 compared with sample tube carrier 215, centrifuge tube carrier 213 is additionally provided with baffle at the rear for the hole position for accommodating centrifuge tube.
In the present invention, fixed loading shelf 216 is not illustrated in detail, and is fixedly installed in the bottom of sample process platform 21
On plate 210, liquid is abandoned for shaking heating, transfer standing and magnetically attractive.
As shown in figure 8, waste holder carrier 217 be removably installed on the bottom plate 210 of sample process platform 21 and with it is solid
Determine carrier 216 and be used for installing waste holder close to, waste holder carrier 217, waste holder include it is multiple be respectively intended to abandon useless suction nozzle and
The chamber of waste liquid (separation of solid and liquid).
When operation, tips is loaded on suction nozzle box carrier, kit each component is put in reagent trough carrier, deep-well plates are put in
PCR plate carrier, is operated by instrument touch screen computer point, is loaded into carrier successively according to the rules, and automated system operation is extremely later
Terminate, directly obtains required nucleic acid extraction product.
Entire sample process process is completed in deep-well plates, is carried out using 4 channels crawl Tips on mechanical arm
Inhale/pipetting processes are abandoned, it carries out shifting between standing/magnetically attractive/concussion disparate modules of deep-well plates simultaneously also by mechanical arm.
The Whole Blood Genomic DNA automatic extracting system of the utility model can greatly reduce the loss of cost and improve work
Make efficiency, after a flux maximum can carry out 96 tests, and machine is run in extraction system completely without human intervention.
The preferred embodiment of the utility model has already been described in detail above, it is understood that having read the utility model
Above-mentioned instruction content after, those skilled in the art can make various changes or modifications the utility model.These shapes of equal value
Formula is also fallen within the scope of the appended claims of the present application.
Claims (10)
1. a kind of Whole Blood Genomic DNA automates extraction system, which is characterized in that including shell, processing unit and control unit,
The processing unit and described control unit are installed in the shell, and the processing unit includes sample process platform and machinery
Arm, the shell is interior to be equipped with guide rail, and the both ends of the guide rail are connect with the inner end wall of the shell, and the mechanical arm is slideably
It is installed on the guide rail, described control unit and the mechanical arm signal communication simultaneously control the mechanical arm on the guide rail
Movement, the sample process platform include bottom plate and be sequentially arranged on the bottom plate suction nozzle box carrier, reagent trough carrier, from
Heart pipe carrier, PCR plate carrier, sample tube carrier, fixed loading shelf and waste holder carrier.
2. Whole Blood Genomic DNA according to claim 1 automates extraction system, which is characterized in that the guide rail is located at
The surface of the sample process platform.
3. Whole Blood Genomic DNA according to claim 1 automates extraction system, which is characterized in that the guide rail distance
The distance of the sample process platform is 4cm-17cm.
4. Whole Blood Genomic DNA according to claim 1 automates extraction system, which is characterized in that the suction nozzle box carry
Frame includes chamber, and support element is equipped in the chamber, and the support element stretches out upwards from the integral middle of the chamber.
5. Whole Blood Genomic DNA according to claim 1 automates extraction system, which is characterized in that the reagent trough carries
Frame includes that elongated bottom is stood equipped with the more root posts stretched out upwards on the bottom of the strip per adjacent two
A kit screens is defined between column.
6. Whole Blood Genomic DNA according to claim 1 automates extraction system, which is characterized in that the centrifuge tube carries
Frame includes carrier main body and handle, and the carrier main body is equipped with multiple holes for placing centrifuge tube and the handle from the load
The end of frame body, which is integrally stretched out, to be formed.
7. Whole Blood Genomic DNA according to claim 1 automates extraction system, which is characterized in that the PCR plate carrier
Equipped with multiple screens, each screens is for placing a PCR plate.
8. Whole Blood Genomic DNA according to claim 1 automates extraction system, which is characterized in that the centrifuge tube carries
Frame includes centrifuge tube carrier main body and handle, and the handle of the centrifuge tube carrier is integrated from the end of the centrifuge tube carrier main body
It stretches out and is formed.
9. Whole Blood Genomic DNA according to claim 1 automates extraction system, which is characterized in that the fixed loading shelf
It is fixedly installed on the bottom plate of the sample process platform, and includes that concussion heating module, transfer standing module and magnetic board are inhaled
Magnetic module.
10. Whole Blood Genomic DNA according to claim 1 automates extraction system, which is characterized in that the waste holder carries
Frame is movably installed on the bottom plate of the sample process platform, and includes multiple being used for being respectively intended to abandon useless suction nozzle and useless
The chamber of liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721337130.XU CN207699585U (en) | 2017-10-17 | 2017-10-17 | Whole Blood Genomic DNA automates extraction system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721337130.XU CN207699585U (en) | 2017-10-17 | 2017-10-17 | Whole Blood Genomic DNA automates extraction system |
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| Publication Number | Publication Date |
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| CN207699585U true CN207699585U (en) | 2018-08-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111704996A (en) * | 2020-06-29 | 2020-09-25 | 杭州医学院 | Intelligent full-automatic biosafety pathogenic microorganism research system |
-
2017
- 2017-10-17 CN CN201721337130.XU patent/CN207699585U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111704996A (en) * | 2020-06-29 | 2020-09-25 | 杭州医学院 | Intelligent full-automatic biosafety pathogenic microorganism research system |
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