CN217516959U - Portable nucleic acid extraction element - Google Patents

Abstract

The utility model relates to the technical field of nucleic acid extraction and purification, in particular to a portable nucleic acid extraction device, which comprises an extraction mechanism, wherein the extraction mechanism comprises a connector connected with a stirring sleeve and a magnetic rod which is arranged corresponding to the connector and is matched with the stirring sleeve to finish extraction operation, and the magnetic rod is driven by a magnetic control unit to finish the operation of stretching into or not stretching into the corresponding stirring sleeve so as to ensure that the outside of the stirring sleeve has magnetic acting force or does not have magnetic acting force; the beneficial effects are as follows: the magnetic force is applied or removed to stretch into or not stretch into the corresponding stirring sleeve, so that the stirring sleeve is externally provided with or does not have the operation of magnetic acting force, the lifting of the magnetic rod under the control of the magnetic force is simply utilized, the reliability of the system is ensured, and the problems of cost and reliability caused by the use of the traditional motor and other schemes are solved.

Description

Portable nucleic acid extraction element

Technical Field

The utility model relates to an extraction and purification technical field of nucleic acid, concretely relates to portable nucleic acid extraction element.

Background

The nucleic acid extractor is an instrument which automatically finishes the work of extracting the nucleic acid of a sample by using a matched nucleic acid extracting reagent. The method is widely applied to various fields of disease control center, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like. Most of full-automatic nucleic acid extractors adopt the extraction principle of a magnetic bead method. The magnetic bead method for extracting nucleic acid is to crack cells by cell lysis solution, utilize the principle that magnetic beads adsorb nucleic acid under high salt and low pH value and separate nucleic acid under low salt and high pH value, so that nucleic acid molecules free from cells are specifically adsorbed to the surfaces of magnetic particles, while protein and other impurities are not adsorbed and remain in solution, then under the action of a magnetic field, the magnetic particles are separated from liquid, particles (namely, a magnetic bead-DNA mixture) are recovered, and then elution is carried out by eluent, so that pure DNA can be obtained.

The automatic nucleic acid extractor using the magnetic bead method in the prior art comprises two types: is applied to a liquid-transferring nucleic acid extractor and a small-sized automatic magnetic rod nucleic acid extractor of a large-sized liquid workstation. The former realizes nucleic acid extraction and purification by adopting a mode of transferring liquid by a mechanical arm, wherein the nucleic acid extraction is only one of the functions of the nucleic acid extraction, and is not suitable for small-flux extraction in a conventional laboratory; the nucleic acid extraction and purification can be realized by the magnetic bar transferring magnetic beads, and the nucleic acid extraction and purification can be realized by arranging two vertical motors to drive the magnetic bar and a stirring sleeve which is sleeved on the magnetic bar to move up and down relatively. However, in the magnetic rod type nucleic acid extractor in the prior art, the magnetic rod and the stirring sleeve move for a long distance from top to bottom, so that the magnetic rod type nucleic acid extractor has a large requirement on the volume of the instrument, and the technical requirement of a small-sized automatic nucleic acid extractor cannot be met; meanwhile, the stirring sleeve always needs to do up-and-down reciprocating motion in the nucleic acid extraction process, so that the stirring sleeve is easy to loosen and even fall off due to vibration in the actual process, the nucleic acid extraction failure is caused, and the extraction instrument can be damaged, so that the extraction instrument cannot work normally.

Therefore, how to satisfy the miniaturization nucleic acid extraction of automated equipment in the fields of biological biochemistry and medical treatment such as molecular diagnosis, the realization of the lowest cost and the simplest extraction driving design is a technical problem to be urgently solved in the field.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a portable nucleic acid extraction element to not enough among the prior art, the device compact structure, small in size can realize the position of operation in-process real-time detection stirring cover, avoid influencing normal work because of stirring cover is not hard up or drops even.

In order to realize the purpose, the utility model provides a technical scheme does:

the utility model provides a portable nucleic acid extraction element, its characterized in that, is including drawing the mechanism, it contains the connector 8 of connecting agitator sleeve 6 to draw the mechanism, still contains corresponding to connector 8 sets up and cooperates agitator sleeve 6 accomplishes the bar magnet 4 who draws the operation, bar magnet 4 is accomplished by the drive of magnetic force control unit and is stretched into or not stretched into correspondingly thereby make in the agitator sleeve 6 the outside has magnetic force or does not have the operation of magnetic force.

Draw the mechanism including stirring cover frame 7, the lower surface of stirring cover frame 7 is connected with stirring cover 6 through a plurality of connectors 8, the last surface mounting of stirring cover frame 7 has bar magnet frame 5, be fixed with a plurality of bar magnets 4 on bar magnet frame 5, it is a plurality of the quantity and the position of bar magnet 4 are corresponding with the quantity and the position of a plurality of stirring cover 6, the upper end of bar magnet 4 is fixed on bar magnet frame 5, the lower extreme of bar magnet 4 aligns with the upper end of connector 8 under initial condition, just bar magnet 4 can pass the inside realization magnetism that the middle part of connector 8 descends to stirring cover 6 and inhale.

The quantity of connector 8 is 16, and the quantity of the bar magnet 4 that corresponds the setting also is 16, the cooperation connect in the stirring cover 6 of connector 8 can accomplish once to the extraction of all samples of a deep hole board.

16 bar magnets 4 are set up to the bar magnet group structure of integral connection, the magnetic force control unit can drive bar magnet group structure integral motion realizes that 16 bar magnets stretch into simultaneously or do not stretch into corresponding stirring cover 6.

The magnetic control unit comprises an electromagnetic chuck, and the magnetic rod 4 is driven to vertically move through electrification or non-electrification, so that the operation of extending into or not extending into the corresponding stirring sleeve 6 is completed.

The device also comprises a translation mechanism for driving the extraction mechanism to move horizontally and a lifting mechanism for driving the extraction mechanism to move vertically.

The translation mechanism comprises a translation motor and a translation screw rod connected with the translation motor, and the translation motor is in driving connection with the extraction mechanism on the translation screw rod to move horizontally.

The lifting mechanism comprises a lifting motor and a screw rod connected with the lifting motor, and the lifting motor drives the extraction mechanism connected to the screw rod to move vertically.

The stirring device also comprises a sensor for detecting the position of the stirring sleeve.

The sensor is a time of flight (TOF) ranging sensor.

Still including the drive the revolution mechanic of 8 movements of connector can realize the stirring to the sample liquid mixing under the stirring cover 6 realization at least rotary motion that 8 drives the connection of connector.

Compared with the prior art, the utility model discloses possess following advantage:

1. the utility model provides a portable nucleic acid extraction device, through controlling its rotation, translation and three kinds of mechanical motion that go up and down, cooperate and draw consumptive material, sample and reactant in the consumptive material, when accomplishing the whole flow of nucleic acid extraction, can reduce the cross contamination in space, improve extraction efficiency simultaneously, guarantee nucleic acid fragment length and completeness in the extraction result;

2. the utility model provides a portable nucleic acid extraction device, which adopts a lifting motor 21 and an electromagnetic chuck 9 to drive a magnetic rod 4 and a stirring sleeve 6 to move vertically, has compact and small structural design, and is suitable for nucleic acid extraction of small flux samples, such as 8 and 16 flux devices;

3. the utility model provides a pair of portable nucleic acid extraction element adopts sensor 11 to be used for 6 positional information of real-time detection stirring cover. Firstly, after identifying the reagent information, detecting and confirming whether the placing position of the stirring sleeve 6 and the position of the reagent hole site of the deep hole plate 27 are correct or not; secondly, detecting and confirming whether the stirring sleeve 6 has a falling risk in the running process; thirdly, detecting and confirming whether the stirring sleeve 6 is unloaded in the corresponding reagent hole after the extraction process is finished;

4. the utility model provides a pair of portable nucleic acid extraction element, through the application of magnetic force or get rid of and accomplish stretch into or not stretch into corresponding in the agitator sleeve, thereby make the agitator sleeve outside has the operation of magnetic force or do not have magnetic force, so realized simply utilizing the lift of magnetic rod under the magnetic control, guaranteed the reliability of system, avoided using cost and reliability problem that scheme such as traditional motor brought, the volume that magnetic control unit has and occupation space are less simultaneously satisfies the strict requirement that portable extraction instrument occupy to the space, further the magnetic control unit is electromagnetic chuck, thereby realized only depending on the operation that the magnetic rod can simply be driven to the impressed current or not impressed current;

5. the utility model provides a pair of portable nucleic acid extraction device, the connector of system and corresponding bar magnet quantity set up to 16 and can once only use this portable nucleic acid extraction appearance to accomplish the simultaneous processing of 16 samples of a deep hole board, guaranteed the high efficiency of extraction appearance, control to the bar magnet of integrated design through the magnetic force control unit, can realize that every operation hole site carries out the same extraction step, guaranteed the reliability of system;

6. the utility model provides a pair of portable nucleic acid extraction element, the translation mechanism of system setting can be one aspect drive and draw the mechanism motion, realizes the connector drive the stirring cover is in the motion between different hole sites, accomplishes whole operation of drawing, cooperates elevating system to accomplish simultaneously and draws the demand of vertical position motion in the operation, has guaranteed that the device satisfies all effects of drawing the operation demand under portable confined space, cooperates TOF type sensor to the real time monitoring of stirring cover state simultaneously, can satisfy probably by minimum interference's effect and system reliable operation under the confined space.

Drawings

For a more clear understanding of the present invention, the present disclosure will be further described by reference to the drawings and illustrative embodiments, which are provided for illustration and are not to be construed as limiting the present disclosure.

FIG. 1 is a first schematic diagram of a partial extraction process driven by a magnetic control unit according to the present invention;

FIG. 2 is a schematic diagram of a part of the extraction process driven by the magnetic control unit of the present invention;

FIG. 3 is a front view of the 16-flux portable nucleic acid extracting apparatus according to the present invention;

FIG. 4 is a back view of the 16-throughput portable nucleic acid isolation apparatus of the present invention;

FIG. 5 is a partial block diagram of the unloader assembly of the present invention;

FIG. 6 is a structural diagram of the device for unloading the stirring sleeve of the present invention;

fig. 7 is the local structure diagram of the mechanism for extracting when the stirring sleeve is unloaded.

Shown in the figure: the automatic discharging device comprises a base 1, a frame 2, a fan 3, a magnetic rod 4, a magnetic rod rack 5, a stirring sleeve 6, a stirring sleeve rack 7, a stirring sleeve grabbing rod 8, an electromagnetic chuck 9, a magnet 10, a sensor 11, an unloading motor 12, a gear 13, a rack 14, a slip sheet 15, a positioning groove 151, a rotating motor 16, a rotating transmission gear 17, a translation motor 18, a translation screw rod 19, a translation guide rail 20, a lifting motor 21, a lifting screw rod 22, a lifting guide rail 23, a discharging motor 24, a discharging guide rail 25, a discharging transmission gear 26 and a deep hole plate 27.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

Example 1

FIGS. 1 and 2 are schematic diagrams showing the details of the processes that may be involved in nucleic acid extraction with a magnetic rod coupled to a stirring sleeve under the drive of a magnetic control unit.

Sample lysis: at least part of a sample to be detected is transferred to a cracking hole site for cracking operation, in order to ensure the completeness of cracking, a stirring sleeve can be immersed in cracking liquid in the cracking hole, magnetic force is applied by a magnetic force control unit at the moment so that a magnetic rod can hardly extend out of a connector part (namely the magnetic rod does not extend into the corresponding stirring sleeve), the stirring sleeve does not have magnetism at the moment, the solution in the cracking hole at the moment is fully stirred through the rotation and/or the vertical oscillation of the stirring sleeve, so that the sample liquid is cracked more thoroughly, at the moment, no magnetic beads exist in the sample liquid, part or all of the magnetic rod can be exposed out of the connector for stirring, the vertical oscillation and stirring which are required for stirring operation in other subsequent steps are taken as examples for explanation, in fact, the vertical oscillation and/or the rotary stirring can be adopted to ensure the uniform mixing effect at any time, the number of consumables in the hole site is extracted from the real number of consumables which are not actually used in the hole site, the figures are also only schematic illustrations of the various operations that may occur within the consumable;

collecting magnetic beads to a lysis well: when the cracking process is finished, the sample liquid can be considered to be cracked fully, at the moment, the magnetic rod with the stirring sleeve is extended into the magnetic bead storage hole, in order to ensure the activity of the magnetic beads and to be more convenient to transfer and the like, the magnetic beads are placed in the magnetic bead preservation solution, before the transfer, the magnetic rod can be fully stirred and uniformly mixed, and then the magnetic rod fully and furthest exceeds the connecting head in the transfer operation (at the moment, the magnetic rod can extend into the stirring sleeve under the action of gravity and the like by the magnetic control unit without applying magnetic force, and the magnetic rod can be matched with the elastic force of a spring and the like to ensure that the magnetic rod more reliably extends into the stirring sleeve when the magnetic control unit does not apply magnetic force, and the position is not limited), therefore, the magnetic force of the magnetic rod (which can be made of a permanent magnet material) influences the environment outside the stirring sleeve to the maximum extent, so that the outside of the stirring sleeve has a magnetic acting force, and the generated magnetic force can more firmly adsorb magnetic beads on the side wall outside the stirring sleeve;

binding of magnetic beads to nucleic acids: then the magnetic bead is driven by the stirring sleeve to be transferred to a cracking hole, then the magnetic control unit applies a magnetic force to retract the magnetic rod to the connector, so that the magnetic influence of the magnetic rod disappears, the magnetic bead is released into a lysis solution, the lysis solution and the magnetic bead are fully mixed by stirring and uniformly mixing of the stirring sleeve, and the magnetic bead can adsorb nucleic acid fragments to complete the combination of the magnetic bead and the nucleic acid fragments;

collecting magnetic beads: then, magnetic beads are collected, the magnetic bar is required to extend out of the connector (deep into the stirring sleeve) to the maximum extent in the magnetic bead collecting operation, and the magnetic beads combined with the nucleic acid fragments are adsorbed on the outer wall of the stirring sleeve under the action of the magnetic bar;

moving magnetic bead value to wash the salt wells and salt wash: the protein and other impurity components can be removed by transferring the nucleic acid to a salt washing hole site and performing salt washing to ensure that the purity of the subsequently extracted nucleic acid is higher; certainly, in the salt washing process, a stirring sleeve with a magnetic rod not extending into the stirring sleeve to form loss of magnetism can be used for stirring and uniformly mixing so as to quickly finish the salt washing process;

collecting magnetic beads to washing wells for three times of washing: after the salt washing operation is completed, transferring the magnetic beads with the nucleic acid fragments to a washing hole position by adopting a similar method, and taking three times of more common washing as an example to explain, firstly, transferring the magnetic beads with the nucleic acid fragments to a washing hole 1, uniformly stirring to complete the first washing, then, transferring the magnetic beads with the nucleic acid fragments to a washing hole 2, uniformly stirring to complete the second washing, finally, transferring the magnetic beads with the nucleic acid fragments to a washing hole 3, uniformly stirring to complete the third washing, wherein after the three times of washing, other impurities which can influence the result basically do not exist;

air-drying, moving the magnetic beads to an elution hole for elution: the magnetic beads with the nucleic acid fragments are slightly controlled to be dried by water under the action of the magnetic force of the magnetic rod, then the magnetic beads can be transferred to an elution hole to carry out elution operation, and the nucleic acid fragments and the magnetic beads are separated by stirring and uniformly mixing under the action of an eluent;

recovering magnetic beads and returning an instrument: and then the magnetic rod stretches into the stirring sleeve to apply magnetic force, so that the magnetic beads separated from the nucleic acid fragments are adsorbed on the wall surface of the stirring sleeve again, and the used magnetic beads are transferred to the previous magnetic bead storage hole.

Of course, in practical applications, some operations may be reduced, omitted, or combined, for example, there may be no salt washing operation or only two times of washing, etc., and it is not limited herein that all functions of all hole sites in the drawings are necessarily included. Certainly, dynamic detection of different steps of operation processes can be achieved through the TOF detecting unit in different processes, whether multiple steps are accurately executed or not is guaranteed to be efficiently obtained in real time, the opposite design can be adopted, the design that the magnetic rod extends into the stirring sleeve when the magnetic force control unit applies magnetic force is adjusted, and the design is not limited in the process.

Example 2

A portable nucleic acid extraction device, as shown in FIGS. 3-7, comprises a base 1, a frame 2, a fan 3, a sample chamber, an extraction mechanism, a translation mechanism and a lifting mechanism; the frame 2 is vertically connected to the base 1, the sample bin is arranged on the base 1, and the deep hole plate 27 is placed in the sample bin; the extracting mechanism is arranged on the frame 2 through a translation guide rail 20 and a lifting guide rail 23, the translation mechanism is used for driving the extracting mechanism to horizontally move along the x-axis direction, and the lifting mechanism is used for driving the extracting mechanism to vertically move along the z-axis direction; draw the mechanism including stirring cover frame 7, the lower surface of stirring cover frame 7 is connected with stirring cover 6 through a plurality of stirring cover connector 8, the last surface mounting of stirring cover frame 7 has bar magnet frame 5, be fixed with a plurality of bar magnets 4 on the bar magnet frame 5, it is a plurality of the quantity and the position of bar magnet 4 are corresponding with the quantity and the position of a plurality of stirring cover 6, the upper end of bar magnet 4 is fixed on bar magnet frame 5, the lower extreme of bar magnet 4 aligns with the upper end of stirring cover connector 8 under initial condition, just bar magnet 4 can pass the middle part that stirring cover connector 8 descends to the bottom realization magnetism of stirring cover 6.

The top of stirring cover frame 7 is installed with revolution mechanic, revolution mechanic includes rotating electrical machines 16 and rotatory drive gear 17, the transmission is connected between rotating electrical machines 16 and the rotatory drive gear 17, the transmission is connected between rotatory drive gear 17 and the stirring cover connector 8. The rotating electrical machines 16 can drive the transmission of rotatory drive gear 17 to the stirring cover connector 8 synchronous revolution that the drive links to each other with rotatory drive gear 17, the revolution mechanic at here can realize stirring cover connector 8 drives stirring cover 6 of connecting and realizes the stirring mixing to sample liquid under the rotary motion at least, and stirring cover 6 at this here also can be above-and-below motion, realizes the stirring under the combined motion, and more even just upper and lower oscillation type stirring effect, this place is not injectd.

The translation mechanism comprises a translation motor 18 and a translation screw rod 19 in transmission connection with the translation motor 18, the translation motor 18 is installed on the base 1, and the translation screw rod 19 is in threaded connection with the extraction mechanism. The translation motor 18 drives the extraction mechanism connected to the translation screw rod 19 to horizontally move on the translation guide rail 20, so that the magnetic rod 4 and the stirring sleeve 6 are driven to horizontally move.

The lifting mechanism comprises a lifting motor 21 and a lifting screw rod 22 in transmission connection with the lifting motor 21, the lifting motor 21 is installed on the frame 2, and the lifting screw rod 22 is in threaded connection with the extraction mechanism. The lifting motor 21 drives the extraction mechanism connected to the lifting screw 22 to move vertically on the lifting guide rail 23, so as to drive the magnetic rod 4 and the stirring sleeve 6 to move vertically.

The extraction mechanism further comprises a magnetic control unit for controlling the magnetic rod 4 to move up and down, the magnetic control unit is an electromagnetic chuck 9, the electromagnetic chuck 9 is matched with a magnet embedded in the magnetic rod frame 5, and the electromagnetic chuck 9 and the magnet on the magnetic rod frame 5 are adsorbed and separated through power on and off. Electromagnet 9 inhales bar magnet rack 5 to magnetic chuck 9 under the circumstances of circular telegram to make bar magnet 4 not stretch into corresponding agitator sleeve 6 in, electromagnet 9 is not under the circumstances of circular telegram, and bar magnet rack 5 is in the effect lower principle of influence factors such as gravity, the magnet separation on electromagnet 9 and the bar magnet rack 5, thereby control bar magnet rack 5 whereabouts, and bar magnet 4 stretches into in the agitator sleeve 6 and makes the agitator sleeve outer wall adsorb the magnetic bead.

Stirring cover attach fitting 8 is 16, and the magnetic rod quantity that corresponds the setting also is 16, the cooperation connect in stirring cover 6 of stirring cover attach fitting 8 can accomplish once to the extraction of all samples of a deep hole board, 16 magnetic rods are set up to the magnetic rod group structure of integral connection (also be the magnetic rod frame 5), magnetic control unit (also be electromagnetic chuck 9) can drive magnetic rod frame 5 overall motion realizes 16 magnetic rods stretch into simultaneously or do not stretch into corresponding in the stirring cover to the portable small size requirement has been satisfied and has been handled to the quick extraction operation of all samples of a deep hole board.

As shown in fig. 6, the extracting mechanism further includes an unloading assembly for unloading the stirring sleeve, the unloading assembly is installed below the stirring sleeve frame 7, the unloading assembly includes an unloading motor 12, the unloading motor 12 is fixedly connected to the frame 2, an output shaft of the unloading motor 12 is in transmission connection with a gear 13, the gear 13 is in meshing connection with a rack 14, a sliding sheet 15 is fixedly connected to a lower surface of the rack 14, and one end of the sliding sheet 15 is provided with a plurality of positioning grooves 151. The uninstallation motor 12 drives the gear 13 to roll on the rack 14 in a meshing manner, so that the sliding sheet 15 is driven to move horizontally until the stirring sleeve connecting joint 8 is clamped into the positioning groove 151 of the sliding sheet 15, the stirring sleeve 6 and the stirring sleeve connecting joint 8 are separated by matching with the lifting mechanism, and the stirring sleeve 6 is uninstalled in the reagent hole in the deep hole plate 27.

The extracting mechanism further comprises a sensor 11 for detecting the position of the stirring sleeve, the sensor 11 is fixed on the unloading assembly, and the sensor 11 detects the position information of the stirring sleeve 6 in the working and running process so as to confirm the state of the stirring sleeve 6, so that the risk that whether the placing position of the stirring sleeve 6 is opposite to the reagent hole position and whether the stirring sleeve loosens or even falls off in the running process is judged. The sensor 11 may be a time of flight (TOF) ranging sensor that emits modulated near infrared light that is reflected after encountering the mixing sleeve, and detects position distance information of the mixing sleeve by calculating a difference between light emission and reflection times.

Nucleic acid extraction element is still including being used for driving the business turn over storehouse mechanism of sample storehouse business turn over, business turn over storehouse mechanism installs on base 1, business turn over storehouse mechanism is including a plurality of delivery drive gear 26 that delivery motor 24 and link to each other with delivery motor 24, and is a plurality of delivery drive gear 26 meshing is connected with delivery guide rail 25, install the sample storehouse on the delivery guide rail 25. The delivery motor 24 drives the delivery transmission gear 26 to rotate so as to drive the sample bin mounted on the delivery guide rail 25 to move along the y-axis direction, so as to drive the deep hole plate 27 on the sample bin to move in and out.

The utility model discloses a theory of operation as follows:

deep hole board 27 is placed in the sample storehouse, vertically place stirring cover 6 in the hole site that deep hole board 27 corresponds, open the extraction element power, the warehouse entry mechanism control sample storehouse is closed, the nucleic acid extraction process begins, at this moment, the initial relative position of bar magnet 4 and stirring cover connector 8 is that the bar magnet 4 lower extreme aligns with stirring cover connector 8 upper end, sensor 11 carries out position information to stirring cover 6 and detects, confirm whether stirring cover 6 places the position and the requirement of deep hole board 27 hole site is correct, after correct according to the procedure operation experiment.

The translation motor 18 drives the extraction mechanism to move above the deep hole plate 27 and the stirring sleeve 6, the lifting motor 21 drives the stirring sleeve frame 7 to descend, the electromagnetic chuck 9 is powered off to drive the magnetic rod frame 5 to descend, so that the stirring sleeve connecting joint 8 enters the stirring sleeve 6 from an opening at the upper end of the stirring sleeve 6, and the automatic grabbing of the stirring sleeve 6 is completed; subsequently, the lifting motor 21 drives the magnetic rod holder 5 and the stirring jacket holder 7 to synchronously lift, thereby driving the grabbed stirring jacket 6 to lift and leave the deep hole plate 27.

The translation motor 18 drives the extraction mechanism to move to the position above a hole position, in which a lysis solution and magnetic beads are pre-packaged, of the deep hole plate 27, the lifting motor 21 drives the stirring sleeve frame 7 to descend, and the lower end of the stirring sleeve 6 extends into the bottom of the deep hole plate 27; the rotating motor 16 drives the rotating transmission gear 17 to rotate, so as to drive the stirring sleeve connecting joint 8 and the stirring sleeve 6 connected with the rotating transmission gear 17 to synchronously rotate; the lifting motor 21 drives the stirring sleeve frame 7 to rotate and vertically move up and down at the same time of rotation, so that the sample and the lysate are stirred and uniformly mixed; after uniform mixing, the nucleic acid substances in the sample are released and gathered on the magnetic beads, the electromagnetic chuck 9 is powered off, the magnetic rod rack 5 descends, the magnetic rod 4 goes deep into the bottom of the stirring sleeve 6 for magnetic absorption, and the magnetic beads in the deep hole plate 27 are gathered at the bottom of the stirring sleeve 6; the lifting motor 21 drives the magnetic rod rack 5 and the stirring sleeve rack 7 to synchronously ascend, so that the stirring sleeve 6 with the magnetic beads is driven to ascend to leave the deep hole plate 27. The extraction mechanism heats the heating strip of the extraction equipment while rotating and mixing uniformly, and provides the heating temperature required by the cracking hole position of the deep hole plate.

The translation motor 18 drives the extraction mechanism to move to the position above the hole position of the deep hole plate 27 prepackaged with the washing liquid, the lifting motor 21 drives the stirring sleeve frame 7 to descend, the lower end of the stirring sleeve 6 extends into the bottom of the deep hole plate 27, and the magnetic beads adsorbed with the nucleic acid and gathered at the lower end of the stirring sleeve 6 are released into the washing liquid of the deep hole plate 27; the rotating motor 16 drives the rotating transmission gear 17 to rotate, so that the stirring sleeve connecting joint 8 connected with the rotating transmission gear 17 and the stirring sleeve 6 are driven to synchronously rotate; the lifting motor 21 drives the stirring sleeve frame 7 to rotate and vertically move up and down at the same time of rotation, so that the uniform mixing speed of the reaction solution is increased, and the uniform mixing effect of the reaction solution is improved; after uniform mixing, impurities except the nucleic acid in the sample are washed, and the purified nucleic acid is still adsorbed on the surface of the magnetic beads; when the electromagnetic chuck 9 is powered off, the magnetic rod frame 5 descends, the magnetic rod 4 goes deep into the bottom of the stirring sleeve 6 for magnetic attraction, and magnetic beads in the washing liquid in the deep hole plate 27 are gathered at the bottom of the stirring sleeve 6; the lifting motor 21 drives the magnetic rod rack 5 and the stirring sleeve rack 7 to synchronously ascend, thereby driving the stirring sleeve 6 with the magnetic beads gathered to ascend away from the deep hole plate 27.

The translation motor 18 drives the extraction mechanism to move to the position above the hole position of the deep hole plate 27 prepackaged with the eluent, the lifting motor 21 drives the stirring sleeve frame 7 to descend, the lower end of the stirring sleeve 6 extends into the bottom of the deep hole plate 27, and the magnetic beads adsorbed with the nucleic acid and gathered at the lower end of the stirring sleeve 6 are released into the eluent of the deep hole plate 27; the rotating motor 16 drives the rotating transmission gear 17 to rotate, so as to drive the stirring sleeve connecting joint 8 and the stirring sleeve 6 connected with the rotating transmission gear 17 to synchronously rotate; the lifting motor 21 drives the stirring sleeve frame 7 to rotate and vertically move up and down at the same time of rotation, so that the uniform mixing speed of the reaction solution is increased, and the uniform mixing effect of the reaction solution is improved; after mixing uniformly, the purified nucleic acid adsorbed on the magnetic beads is released into the eluent; when the electromagnetic chuck 9 is powered off, the magnetic rod frame 5 descends, the magnetic rod 4 goes deep into the bottom of the stirring sleeve 6 for magnetic attraction, and magnetic beads in the washing liquid in the deep hole plate 27 are gathered at the bottom of the stirring sleeve 6; the lifting motor 21 drives the magnetic rod frame 5 and the stirring sleeve frame 7 to synchronously ascend, so that the stirring sleeve 6 with the magnetic beads is driven to ascend to leave the deep hole plate. The extraction mechanism heats the heating strip of the extraction equipment while rotating and mixing uniformly, and provides the heating temperature required by the elution hole position of the deep hole plate.

The translation motor 18 drives the extraction mechanism to move to the position above the hole site of the deep hole plate 27, which is originally pre-packaged with lysate and magnetic beads, the lifting motor 21 drives the stirring sleeve frame 7 to descend, the electromagnetic chuck 9 is powered off to drive the magnetic rod frame 5 to descend, the stirring sleeve 6 extends into the deep hole plate 27, and the unloading motor 12 drives the gear 13 to rotate so as to drive the sliding sheet 15 to translate to the position where the stirring sleeve connecting joint 8 is clamped into the sliding sheet 15; lifting motor 21 drives stirring cover frame 7 and bar magnet frame 5 and rises, and the in-process that rises, 6 upper ends of stirring cover will withstand positioning groove 151, and the stirring cover 6 that forces the bottom gathering to have the magnetic bead is forced to be left over in the deep hole board, and sensor 11 detects stirring cover 6 positional information, confirms whether stirring cover 6 has left over in deep hole board 27. At this point, the device is finished and the nucleic acid extraction product in the deep well plate 27 can be used in downstream detection and analysis experiments.

In the above working process, the sensor 11 will detect the position state of the stirring sleeve 6 in operation in real time, and determine whether the stirring sleeve 6 has a risk of loosening or even falling off.

It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (9)

1. A portable nucleic acid extraction device is characterized by comprising an extraction mechanism, wherein the extraction mechanism comprises a connector (8) connected with a stirring sleeve (6), and further comprises a magnetic rod (4) which is arranged corresponding to the connector (8) and matched with the stirring sleeve (6) to complete extraction operation, and the magnetic rod (4) is driven by a magnetic control unit to complete the operation of extending into or not extending into the corresponding stirring sleeve (6) so as to enable the outer part of the stirring sleeve (6) to have magnetic acting force or not to have magnetic acting force; draw mechanism including stirring cover frame (7), the lower surface of stirring cover frame (7) is connected with stirring cover (6) through a plurality of connectors (8), the last surface mounting of stirring cover frame (7) has bar magnet frame (5), be fixed with a plurality of bar magnets (4) on bar magnet frame (5), it is a plurality of the quantity and the position of bar magnet (4) are corresponding with the quantity and the position of a plurality of stirring cover (6), the upper end of bar magnet (4) is fixed on bar magnet frame (5), the lower extreme of bar magnet (4) aligns with the upper end of connector (8) under initial state, just bar magnet (4) can pass the inside realization magnetism that the middle part of connector (8) descends to stirring cover (6) and inhale.

2. The portable nucleic acid extraction device according to claim 1, wherein: the quantity of connector (8) is 16, and the quantity that corresponds bar magnet (4) that sets up also is 16, the cooperation connect in the stirring cover (6) of connector (8) can be accomplished once to the extraction of all samples of a deep hole board.

3. The portable nucleic acid extraction device according to claim 2, wherein: 16 bar magnet (4) are set up to the bar magnet group structure of integral connection, the magnetic force control unit can drive bar magnet group structure global motion realizes that 16 bar magnets stretch into simultaneously or do not stretch into corresponding stirring cover (6).

4. The portable nucleic acid extraction device according to claim 1, wherein: the magnetic control unit comprises an electromagnetic chuck, and the magnetic rod (4) is driven to vertically move through electrification or non-electrification to complete the operation of extending into or not extending into the corresponding stirring sleeve (6).

5. The portable nucleic acid extraction device according to claim 1, wherein: the device also comprises a translation mechanism for driving the extraction mechanism to move horizontally and a lifting mechanism for driving the extraction mechanism to move vertically.

6. The portable nucleic acid extraction device according to claim 5, wherein: the translation mechanism comprises a translation motor and a translation screw rod connected with the translation motor, and the translation motor drives an extraction mechanism connected to the translation screw rod to move horizontally; the lifting mechanism comprises a lifting motor and a screw rod connected with the lifting motor, and the lifting motor drives the extraction mechanism connected to the screw rod to move vertically.

7. The portable nucleic acid extraction device according to claim 1, wherein: the stirring device also comprises a sensor for detecting the position of the stirring sleeve.

8. The portable nucleic acid extraction device according to claim 7, wherein: the sensor is a time of flight (TOF) ranging sensor.

9. The portable nucleic acid extraction device according to claim 1, wherein: still including the drive the revolution mechanic of connector (8) motion can realize connector (8) drive stirring cover (6) of connecting realize the stirring mixing to sample liquid under the at least rotary motion.

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