CN112011533A - Integrated system for extracting and detecting nucleic acid and detection method - Google Patents
Integrated system for extracting and detecting nucleic acid and detection method Download PDFInfo
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- CN112011533A CN112011533A CN201910394313.2A CN201910394313A CN112011533A CN 112011533 A CN112011533 A CN 112011533A CN 201910394313 A CN201910394313 A CN 201910394313A CN 112011533 A CN112011533 A CN 112011533A
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Abstract
The invention provides an integrated system for extracting and detecting nucleic acid and a detection method, wherein the system comprises a sample processing unit, a sample detection unit and a sample detection unit, wherein the sample processing unit comprises at least one cracking unit, at least one washing unit and at least one elution unit; a liquid supply unit; the liquid feeding unit is positioned above the sample processing unit and is provided with a liquid feeding device, the liquid feeding unit is respectively connected with the liquid feeding driving unit and the liquid feeding unit, and the liquid feeding driving unit is provided with a horizontal moving mechanism and a vertical moving mechanism; a magnetic attraction bar unit; the detection unit is positioned on one side of the elution unit and is provided with a detection channel for detecting by the elution unit. The system combines the separation, extraction and detection of nucleic acid into a whole, realizes the continuous automatic operation of the separation, extraction and detection of nucleic acid to a certain extent, has simple operation steps, improves the efficiency and improves the accuracy of detection results.
Description
Technical Field
The invention belongs to the technical field of nucleic acid extraction and detection, and particularly relates to an integrated system and a detection method for nucleic acid extraction and detection.
Background
Nucleic acid is a basic object of molecular biology research, and the separation and extraction of nucleic acid is a very important link in the whole molecular biology research process, and the extraction of nucleic acid cannot be separated in the aspects of microbial virus detection, gene screening and recombination, clinical diagnosis and treatment. At present, common nucleic acid extraction methods mainly include an organic solvent extraction method, a silica gel column adsorption method, a magnetic bead method, a charge method and the like. Among them, the magnetic bead method is widely used and has high automation degree. The basic principle of the magnetic bead method is that nanometer magnetic bead microbeads are adopted to selectively adsorb nucleic acid, magnetic beads adsorbing the nucleic acid are enriched and separated under an external magnetic field, the enriched magnetic beads are cleaned, impurities and other unnecessary substances are further removed, and finally the nucleic acid enriched on the magnetic beads is released into a required solution system under certain conditions.
The nucleic acid extraction equipment for changing the uniform mixing mode disclosed in Chinese patent document CN106119082A comprises a base, a test tube rack, a magnetic rod sleeve frame, a magnetic rod sleeve driving mechanism and a magnetic rod driving mechanism, wherein the test tube rack, the magnetic rod sleeve frame and the magnetic rod frame are arranged on the base, the magnetic rod sleeve driving mechanism and the magnetic rod driving mechanism respectively drive the magnetic rod sleeve frame and the magnetic rod frame to independently act, the nucleic acid extraction equipment further comprises a horizontal moving mechanism arranged on the base, the horizontal moving mechanism drives the magnetic rod sleeve driving mechanism and the magnetic rod driving mechanism to horizontally move, so that the magnetic rod sleeve and the magnetic rod horizontally move, and the magnetic rod sleeve horizontally reciprocates to uniformly mix reagents. The horizontal moving mechanism enables the magnetic rod sleeve to transversely move back and forth in the lysis solution cavity, so that large-volume lysis solution, nucleic acid and magnetic beads can be fully and uniformly mixed, the magnetic beads can fully and uniformly adsorb the nucleic acid, the magnetic beads can be extracted under the action of the magnetic rod sleeve driving mechanism and the magnetic rod driving mechanism through the magnetic rod sleeve and the magnetic rod, the purpose of extracting the nucleic acid with high purity and high efficiency is finally achieved, and a DNA long chain of the nucleic acid is kept as far as possible. However, in the above scheme, the nucleic acid extraction equipment with the changed mixing mode is only an automatic equipment for separating nucleic acid by combining a magnetic bead method, that is, only the automatic transfer of magnetic beads can be realized, on one hand, the liquid inlet of each step in the nucleic acid separation and extraction is not taken into consideration, manual liquid adding is adopted, the operation is complex and low in efficiency, and the liquid is easily added by mistake in the operation process, on the other hand, the magnetic bead separation and the nucleic acid detection of nucleic acid are not integrated, so that the continuous automatic operation of nucleic acid separation, extraction and detection cannot be realized, so that when the nucleic acid after the elution step is detected, a test tube needs to be taken out from a test tube rack and then sent into a nucleic acid detection instrument for detection, the operation steps are complex, the efficiency is low, and in the detection process, the nucleic acid eluent in the test tube is easily polluted or spilled.
Disclosure of Invention
Therefore, the technical problem to be solved by the present invention is that the nucleic acid separation and extraction device in the prior art has a single function, and cannot realize continuous automatic operation of nucleic acid separation, extraction and detection, resulting in complicated operation steps, low efficiency and inaccurate detection result, thereby providing an integrated system for nucleic acid extraction and detection and a detection method.
To this end, the present invention provides an integrated system of an integrated system for nucleic acid extraction and detection, comprising:
a sample processing unit comprising at least one lysis unit, at least one washing unit and at least one elution unit;
a liquid supply unit for supplying a required liquid to the sample processing unit;
the liquid feeding unit is positioned above the sample processing unit and is provided with a liquid feeding device, the liquid feeding unit is respectively connected with the liquid feeding driving unit and the liquid feeding unit, and the liquid feeding driving unit is provided with a horizontal moving mechanism and a vertical moving mechanism;
the magnetic rod driving unit and the magnetic rod sleeve driving unit are respectively provided with a horizontal moving mechanism and a vertical moving mechanism;
the detection unit is positioned on one side of the elution unit and is provided with a detection channel for the elution unit to detect.
The integrated system for extracting and detecting nucleic acid also comprises at least one cleaning unit in the sample processing unit.
The integrated system for extracting and detecting the nucleic acid is characterized in that the liquid adding device is a liquid adding needle.
The integrated system for extracting and detecting nucleic acid, the magnetic rod sleeve driving unit also comprises a vibration mechanism.
The integrated system for extracting and detecting nucleic acid is characterized in that a lysis unit, a washing unit and an elution unit are sequentially arranged in the sample processing unit along the horizontal direction.
The integrated system for extracting and detecting nucleic acid is characterized in that 1-12 washing units are arranged in the sample processing unit along the horizontal direction.
The integrated system for extracting and detecting the nucleic acid is characterized in that the elution unit is a cuvette, and the detection unit is an ultraviolet spectrophotometry detection unit.
The method for extracting and detecting the nucleic acid by the integrated system for extracting and detecting the nucleic acid comprises the following steps:
s1, adding a sample to be detected and magnetic beads into a cracking unit, and adding corresponding cracking liquid, washing liquid and eluent into the cracking unit, a washing unit and an elution unit in a sample processing unit by using a liquid adding unit;
s2, adsorbing and transferring magnetic beads by using the magnetic sucker unit, and sequentially transferring the magnetic beads from the cracking unit to the washing unit and the elution unit, wherein the magnetic beads are sequentially cracked, washed and eluted;
s3, after the elution of the magnetic beads in the to-be-eluted unit is completed, the magnetic beads are adsorbed by the magnetic rod unit and transferred to the eluting unit, and the eluting unit is transferred to the detection channel of the detection unit for detection.
The method further comprises the step of flushing a liquid adding device in the liquid adding unit before the liquid adding unit is used for adding liquid to the sample processing unit in the step S1.
In the method, in step S2, before transferring the magnetic beads, the mixture of the magnetic beads and the liquid is uniformly mixed by vibration using the magnetic rod sleeve.
The technical scheme of the invention has the following advantages:
1. the invention provides an integrated system for extracting and detecting nucleic acid, which comprises: a sample processing unit comprising at least one lysis unit, at least one washing unit and at least one elution unit; a liquid supply unit for supplying a required liquid to the sample processing unit; the liquid feeding unit is positioned above the sample processing unit and provided with a liquid feeding device, the liquid feeding unit is respectively connected with the liquid feeding driving unit and the liquid feeding unit, and the liquid feeding driving unit drives the liquid feeding unit to move horizontally and vertically; the magnetic rod driving unit and the magnetic rod sleeve driving unit are respectively provided with a horizontal moving mechanism and a vertical moving mechanism; the detection unit is positioned on one side of the elution unit and is provided with a detection channel for the detection of the elution unit; through set up sample processing unit, supply liquid unit, liquid feeding unit, magnetism inhale excellent unit and detecting element in above-mentioned system, supply the liquid unit for the required liquid of liquid feeding unit supply, the liquid feeding unit can carry out the liquid feeding to schizolysis unit, washing unit and elution unit among the sample processing unit automatically, magnetism is inhaled excellent unit and can be automatic handles the sample processing unit that adds the liquid, shifts the magnetic bead from the schizolysis unit to washing unit and elution unit in proper order, and after the magnetic bead elutes at the elution unit, can shift the elution unit to detecting element's testing channel and detect, has realized liquid feeding, magnetic bead absorption, washing and elution, nucleic acid detection's full automatization, easy operation is convenient, efficient and the testing result is accurate.
2. The invention provides an integrated system for extracting and detecting nucleic acid, wherein the sample processing unit also comprises at least one cleaning unit; the cleaning unit is used for containing and cleaning waste liquid of the liquid adding unit.
3. The invention provides an integrated system for extracting and detecting nucleic acid, wherein the magnetic rod sleeve driving unit also comprises a vibration mechanism; the vibration mechanism can drive the magnetic rod sleeve to vibrate, and further can be used for utilizing the magnetic rod sleeve to play a stirring function.
4. The integrated system for extracting and detecting nucleic acid provided by the invention comprises a sample processing unit, a sample washing unit and a sample washing unit, wherein the sample processing unit is internally provided with the lysis unit, the washing unit and the elution unit in sequence along the horizontal direction; through the unit order that sets up in the sample processing unit, aim at makes things convenient for the automation mechanized operation of liquid feeding unit and magnetism to inhale the stick unit.
5. The method for extracting and detecting the nucleic acid by using the integrated system for extracting and detecting the nucleic acid is simple and easy to operate, and has high automation degree and high efficiency.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a block diagram of an integrated nucleic acid extraction and detection system according to an embodiment of the present invention;
FIG. 2 is a top view of a sample processing cell of one embodiment of the present invention.
Reference numerals:
1-sample processing unit, 111-cracking unit, 112-washing unit, 113-elution unit, 2-liquid supply unit, 211-reagent bottle, 212-peristaltic pump, 3-liquid adding unit, 4-liquid adding driving unit, 5-magnetic rod attracting unit, 511-magnetic rod, 512-magnetic rod sleeve, 6-magnetic rod driving unit, 7-magnetic rod sleeve driving unit, 8-detection unit, 811-detector, 812-light source, 813-monochromator, 9-cleaning unit and 10-control system.
Detailed Description
The invention provides an integrated system for extracting and detecting nucleic acid, which is shown in figure 1-2 and comprises: the device comprises a sample processing unit 1, a liquid supply unit 2, a liquid supply unit 3, a magnetic sucker unit 5 and a detection unit 8;
a sample processing unit 1, where the sample processing unit 1 includes at least one lysis unit 111, at least one washing unit 112, and at least one elution unit 113, and in this embodiment, as shown in fig. 2, the sample processing unit 1 includes 1 lysis unit 111, 11 washing units 112, and 1 elution unit 113 in sequence from left to right along a horizontal direction, and includes 8 sets of the sample processing units 1 along a longitudinal direction, the lysis unit 111 is a sample bottle, the washing unit 112 is a reagent tube, the reagent tube is placed in a reagent tube rack, the elution unit 113 is a cuvette, and the cuvette is inserted in a rack in which the cuvette can be placed; in this embodiment, along the horizontal direction, set up schizolysis unit 111, washing unit 112 and elution unit 113 with sample processing unit 1 order, the aim at is convenient for add liquid unit 3 and magnetism inhale the automated processing of excellent unit 5, add liquid unit 3 can be located elution unit 113's one side can be from the right side to left side and carry out the liquid feeding to elution unit 113, washing unit 112 and schizolysis unit 111 in proper order, magnetism is inhaled excellent unit 5 and can be located cracking unit 111's one side can be from a left side to right side and is handled schizolysis unit 111, washing unit 112 and elution unit 113 in proper order. The sample processing unit 1 may be provided with 1 to 12 washing units in the horizontal direction, and in this embodiment, 11 washing units may be provided to wash the magnetic beads having nucleic acids adsorbed thereon for a plurality of times;
the liquid supply unit 2 is used for supplying required liquid to the sample processing unit 1, in this embodiment, as shown in fig. 1, the liquid supply unit 2 includes reagent bottles 211 containing different liquids, in the figure, the reagent bottles 211 include 4, the reagent bottles 211 can respectively contain lysis buffer, washing solution, eluent and pure water, the lysis buffer is used for adding into the lysis unit 111, the washing solution is used for adding into the washing unit 112, the eluent is used for adding into the elution unit 113, the pure water is used for cleaning a liquid adding device in the liquid adding unit 3, the reagent bottles 211 are connected with the peristaltic pump 212, and the liquid in the reagent bottles 211 is pumped into the liquid adding unit 3 through the peristaltic pump 212;
the liquid adding unit 3 is positioned above the sample processing unit 1, the liquid adding unit 3 is provided with a liquid adding device, the liquid adding unit 3 is respectively connected with a liquid adding driving unit 4 and the liquid supplying unit 2, in the embodiment, the liquid adding unit 3 is connected with a peristaltic pump 212 in the liquid supplying unit 2, when liquid is selected to be supplied to the liquid adding unit 3, a corresponding reagent bottle in the supplying unit 3 is communicated with the liquid adding unit, when the liquid is stopped to be supplied to the liquid adding unit 3, the corresponding reagent bottle in the supplying unit 3 is disconnected from the liquid adding unit, the liquid adding driving unit 4 is provided with a horizontal moving mechanism and a vertical moving mechanism, and the liquid adding driving unit 4 can drive the liquid adding unit 3 to move horizontally or vertically; in this embodiment, the liquid adding device is a liquid adding needle;
a magnetic rod unit 5, the magnetic rod unit 5 being located above the sample processing unit 1, the magnetic rod unit 5 including a magnetic rod 511 and a magnetic rod sleeve 512, the magnetic rod 511 being located above the magnetic rod sleeve 512, the magnetic rod 511 being insertable into the magnetic rod sleeve 512, the magnetic rod 511 being connected to a magnetic rod 6 driving unit, the magnetic rod sleeve 512 being connected to a magnetic rod sleeve driving unit 7, the magnetic rod driving unit 6 and the magnetic rod sleeve driving unit 7 having a horizontal movement mechanism and a vertical movement mechanism, respectively, the magnetic rod driving unit 6 being capable of driving the magnetic rod 511 to move horizontally or vertically, and the magnetic rod sleeve driving unit 7 being capable of driving the magnetic rod sleeve 512 to move horizontally or vertically;
a detecting unit 8, the detecting unit 8 is located at one side of the elution unit 113, the detecting unit 8 has a detecting channel for detecting by the elution unit 113, in the present embodiment, the detecting unit 8 is an ultraviolet spectrophotometric detecting unit, the ultraviolet spectrophotometric detecting unit includes components necessary for ultraviolet-visible spectrophotometric detection, such as a light source, a monochromator, an absorption cell, a detector, a signal processor, and the like, as shown in fig. 2, the ultraviolet spectrophotometric detecting unit is located at one side along the longitudinal moving direction of the elution unit 113, the detector 811 in the ultraviolet spectrophotometric detecting unit is located at the left side of the longitudinal moving direction of the elution unit 113, the monochromator 813 in the ultraviolet spectrophotometric detecting unit is located at the right side opposite to the detector 811, and a gap capable of accommodating the elution unit 113 is provided between the monochromator 813 and the detector 811, the gap is a detection channel for the elution unit 113 to detect, and a light source 812 is provided at the right side of the monochromator 813.
Further, the sample processing unit 1 further includes at least one cleaning unit 9, and in this embodiment, includes 1 cleaning unit, as shown in fig. 2, the cleaning unit 9 is located between the lysis unit and the washing unit, and the cleaning unit 9 is a cleaning tank. The cleaning unit 9 is used for containing waste liquid of a flushing liquid adding device such as a liquid adding needle, so that when the liquid adding device adds liquid to a new unit, liquid adding liquid of other units is still remained in the liquid adding device, and extraction of nucleic acid is not affected.
Further, the bar cover driving unit 7 further includes a vibration mechanism provided to allow the bar cover 512 to vibrate by the vibration mechanism, and when stirring is required, the bar cover 512 is inserted into the liquid to drive the bar cover 512 to perform a stirring function.
Further, the liquid adding driving unit 4, the magnetic rod driving unit 6 and the magnetic rod sleeve driving unit 7 are respectively connected with a control system 10, and the liquid adding driving unit 4, the magnetic rod driving unit 6 and the magnetic rod sleeve driving unit 7 are controlled to be driven by the control system 10.
The embodiment also provides a method for extracting and detecting nucleic acid by using the integrated system for extracting and detecting nucleic acid, which comprises the following steps:
s1, adding a sample to be tested and magnetic beads into a cracking unit 111, wherein in an initial state, the connection between the liquid adding unit 3 and a reagent bottle in the liquid supply unit 2 is in a closed state, the liquid adding unit 3 is communicated with an eluent reagent bottle 211 in the liquid supply unit 2, eluent is pumped into a liquid adding device through a peristaltic pump 212, then the liquid adding driving unit 4 is used for driving a liquid adding device in the liquid adding unit 3 to horizontally move to the upper part of an elution unit 113, then the liquid adding device is driven to vertically move downwards, the liquid adding device is started to add eluent into the elution unit 113, after liquid adding is completed, the liquid adding device is closed, then the liquid adding device is driven to vertically move upwards, the liquid adding device is driven to horizontally move to the next elution unit 113 along the longitudinal direction for liquid adding work, and after all elution units are completely added with liquid, close the intercommunication of liquid feeding device and eluant reagent bottle 211, open the liquid feeding device with be connected between washing liquid (the washing liquid can be the pure water) reagent bottle 211, then the drive the liquid feeding device removes the top of cleaning unit 9, carries out vertical downstream operation, then opens the liquid feeding device, during washing liquid washes remaining eluant in the liquid feeding device to cleaning unit 9, then closes the liquid feeding device, drive the vertical upward movement of liquid feeding device, along the horizontal direction drive the liquid feeding device horizontal migration carries out the liquid feeding work to washing unit 112, circulates according to this, after the liquid feeding unit finishes to the liquid feeding of the unit of the same kind, promptly wash the liquid feeding unit, wash the unit of new kind again after washing, utilize liquid feeding unit 3 will in proper order to elution unit 113 in the sample processing unit 1, Corresponding eluent, washing liquid and lysis solution are added into the washing unit 112 and the lysis unit 111;
s2, adsorbing and transferring magnetic beads by a magnetic rod unit 5, wherein the magnetic rod unit 5 is located at one side of a lysis unit 111, the magnetic rod 511 and the magnetic rod sleeve 512 are in a separated state, the magnetic rod 511 is located above the magnetic rod sleeve 512, the magnetic rod driving unit 6 drives the magnetic rod 511 to move above the lysis unit 111, the magnetic rod sleeve driving unit 7 drives the magnetic rod sleeve 512 to move above the same lysis unit 111, drives the magnetic rod sleeve 512 to vertically move downwards and insert into the lysis unit 111, then drives the magnetic rod 511 to insert into the magnetic rod sleeve 512, the magnetic beads with nucleic acids adsorbed on the surface in the lysis unit 111 are adsorbed on the surface of the magnetic rod sleeve 512, then drives the magnetic rod 511 and the magnetic rod sleeve 512 to simultaneously move vertically upwards and move out of the lysis unit 111, then drives the magnetic rod 511 and the magnetic rod sleeve 512 to horizontally move above the washing unit 112, then, the magnetic rod 511 and the magnetic rod sleeve 512 are driven to vertically move downwards and inserted into the washing unit 112, during which the magnetic rod 511 is kept in a state of being inserted into the magnetic rod sleeve 512, after the magnetic rod 511 and the magnetic rod sleeve 512 are inserted into the washing unit 112, the magnetic rod 511 is driven to vertically move upwards and removed from the washing unit, the magnetic beads adsorbed on the surface of the magnetic rod sleeve 512 are released into the washing liquid, the magnetic rod sleeve 512 can be vibrated by using a vibration mechanism in a magnetic rod sleeve driving unit 7, the mixture of the magnetic beads and the washing liquid is stirred, then the magnetic rod 511 is inserted into the magnetic rod sleeve 512, the magnetic beads are adsorbed again on the surface of the magnetic rod sleeve 512, then the magnetic rod 511 and the magnetic rod sleeve 512 are simultaneously driven to vertically move upwards and removed from the lysis unit 111, and then the magnetic rod 511 and the magnetic rod sleeve 512 are driven to horizontally move to the next unit, according to the circulation, the magnetic beads are sequentially transferred from the cracking unit 111 to the washing unit 112 and the elution unit 113 by using the magnetic rods 511 and the magnetic rod sleeves 512, and the magnetic beads are sequentially cracked, washed and eluted;
s3, after the elution of the magnetic beads in the unit to be eluted 113 is completed, the magnetic beads are adsorbed and transferred out of the elution unit 113 by the magnetic sucker unit 5, and the elution unit 113 is transferred to the detection channel of the detection unit 8 for detection, so that the extraction and detection of nucleic acid are realized.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. An integrated system for nucleic acid extraction and detection, comprising:
a sample processing unit comprising at least one lysis unit, at least one washing unit and at least one elution unit;
a liquid supply unit for supplying a required liquid to the sample processing unit;
the liquid feeding unit is positioned above the sample processing unit and is provided with a liquid feeding device, the liquid feeding unit is respectively connected with the liquid feeding driving unit and the liquid feeding unit, and the liquid feeding driving unit is provided with a horizontal moving mechanism and a vertical moving mechanism;
the magnetic rod driving unit and the magnetic rod sleeve driving unit are respectively provided with a horizontal moving mechanism and a vertical moving mechanism;
the detection unit is positioned on one side of the elution unit and is provided with a detection channel for the elution unit to detect.
2. The integrated nucleic acid extraction and detection system of claim 1, further comprising at least one washing unit in the sample processing unit.
3. The integrated system for nucleic acid extraction and detection according to claim 1 or 2, wherein the liquid adding device is a liquid adding needle.
4. The integrated nucleic acid extraction and detection system according to any one of claims 1 to 3, wherein the magnetic sheath driving unit further comprises a vibration mechanism.
5. The integrated nucleic acid extraction and detection system according to any one of claims 1 to 4, wherein the sample processing unit is provided with a lysis unit, a washing unit and an elution unit in order along a horizontal direction.
6. The integrated nucleic acid extraction and detection system of any one of claims 1 to 5, wherein 1 to 12 washing units are disposed in the sample processing unit in a horizontal direction.
7. The integrated nucleic acid extraction and detection system according to any one of claims 1 to 6, wherein the elution unit is a cuvette and the detection unit is an ultraviolet spectrophotometric detection unit.
8. A method for extracting and detecting nucleic acid using the integrated system for extracting and detecting nucleic acid according to any one of claims 1 to 7, comprising the steps of:
s1, adding a sample to be detected and magnetic beads into a cracking unit, and adding corresponding cracking liquid, washing liquid and eluent into the cracking unit, a washing unit and an elution unit in a sample processing unit by using a liquid adding unit;
s2, adsorbing and transferring magnetic beads by using the magnetic sucker unit, and sequentially transferring the magnetic beads from the cracking unit to the washing unit and the elution unit, wherein the magnetic beads are sequentially cracked, washed and eluted;
s3, after the elution of the magnetic beads in the to-be-eluted unit is completed, the magnetic beads are adsorbed by the magnetic rod unit and transferred to the eluting unit, and the eluting unit is transferred to the detection channel of the detection unit for detection.
9. The method of claim 8, further comprising the step of flushing the priming device in the priming cell prior to priming the sample processing cell with the priming cell in step S1.
10. The method of claim 8 or 9, wherein the magnetic bead and the liquid mixture are mixed by vibrating the magnetic rod sleeve before transferring the magnetic beads in step S2.
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| CN201910394313.2A CN112011533A (en) | 2019-05-13 | 2019-05-13 | Integrated system for extracting and detecting nucleic acid and detection method |
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| CN201910394313.2A CN112011533A (en) | 2019-05-13 | 2019-05-13 | Integrated system for extracting and detecting nucleic acid and detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112795564A (en) * | 2021-03-26 | 2021-05-14 | 苏州博捷特生物科技有限公司 | Rapid total nucleic acid extraction method and kit |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006006258A (en) * | 2004-06-29 | 2006-01-12 | Fuji Photo Film Co Ltd | Method for extracting nucleic acid and apparatus for extracting nucleic acid |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN208815031U (en) * | 2018-05-21 | 2019-05-03 | 南京英芮诚麦歌生化仪器有限公司 | A kind of instrument for extracting nucleic acid continuous liquid adding device |
-
2019
- 2019-05-13 CN CN201910394313.2A patent/CN112011533A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006006258A (en) * | 2004-06-29 | 2006-01-12 | Fuji Photo Film Co Ltd | Method for extracting nucleic acid and apparatus for extracting nucleic acid |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN208815031U (en) * | 2018-05-21 | 2019-05-03 | 南京英芮诚麦歌生化仪器有限公司 | A kind of instrument for extracting nucleic acid continuous liquid adding device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112795564A (en) * | 2021-03-26 | 2021-05-14 | 苏州博捷特生物科技有限公司 | Rapid total nucleic acid extraction method and kit |
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