CN208815031U - A kind of instrument for extracting nucleic acid continuous liquid adding device - Google Patents

A kind of instrument for extracting nucleic acid continuous liquid adding device Download PDF

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Publication number
CN208815031U
CN208815031U CN201820758201.1U CN201820758201U CN208815031U CN 208815031 U CN208815031 U CN 208815031U CN 201820758201 U CN201820758201 U CN 201820758201U CN 208815031 U CN208815031 U CN 208815031U
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China
Prior art keywords
liquid feeding
liquid
pin group
feeding pin
fixing seat
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Application number
CN201820758201.1U
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Chinese (zh)
Inventor
尚春庆
黄烽
严勇
李敬清
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SUZHOU ENRICHING BIOTECHNOLOGY CO.,LTD.
Original Assignee
Nanjing Ying Rui Cheng Song Biochemical Instrument Co Ltd
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Abstract

The utility model relates to a kind of instrument for extracting nucleic acid continuous liquid adding devices, including plunger pump, controller, hose, liquid feeding pin group and fixing seat, liquid feeding pin group is that several liquid feeding pins are uniformly distributed and interconnect, fixing seat is equipped with type chamber compatible with liquid feeding pin group, the setting of liquid feeding pin group is intracavitary in fixing seat type, the syringe needle of liquid feeding pin passes through fixing seat, plunger pump is equipped with multiple inlets, each inlet is connected respectively to different liquid lines, liquid outlet is connect with liquid feeding pin group by hose, the control switch of controller connection plunger pump, it the advantage is that, whole quantitative liquid feeding, avoid manual operation bring error, realize higher extraction efficiency.Micro liquid feeding blending system draws 20-150ul liquid every time, and different liquid needed for mixing well experiment avoid cross contamination when artificial liquid feeding.The degree of automation is higher, does not need manual operation, and after setting nucleic acid extraction program, instrument autonomous operation avoids the fault that manual operation may cause.

Description

A kind of instrument for extracting nucleic acid continuous liquid adding device
Technical field
The utility model relates to a kind of instrument for extracting nucleic acid continuous liquid adding devices.
Background technique
Nucleic acid extraction is the basis of many biological experiments, at present the method for nucleic acid extraction have organic solvent precipitation method, from Stem method and paramagnetic particle method, wherein paramagnetic particle method instrument for extracting nucleic acid is more and more people as a kind of instrument that automation is high-throughput It is selected.But existing instrument for extracting nucleic acid does not have the function of continuous liquid feeding, and cleaning solution washing salt is needed in nucleic acid extraction And the impurity such as protein, current method are expended a large amount of by the way that cleaning solution/eluent etc. is manually added 96 orifice plates in advance Manpower and material resources, it is also possible to because liquid feeding is uneven caused by artificial origin, influence experimental result, increase the uncertain of experimental result Property.
Utility model content
The purpose of this utility model is to provide a kind of continuous liquid feedings of the instrument for extracting nucleic acid that can be realized automatic liquid-feeding to fill It sets.
To achieve the above object, the instrument for extracting nucleic acid continuous liquid adding device of the utility model, connect with work arm, It is characterized by comprising plunger pump, controller, hose, liquid feeding pin group and fixing seat, the liquid feeding pin group is several liquid feeding pins It is uniformly distributed and interconnects, the fixing seat is equipped with type chamber compatible with liquid feeding pin group, and the liquid feeding pin group setting exists Fixing seat type is intracavitary, and the syringe needle of liquid feeding pin passes through fixing seat, and the plunger pump is equipped with multiple inlets and a liquid outlet, each Inlet is connected respectively to different liquid lines, and liquid outlet is connect with liquid feeding pin group by hose, and the controller connects plunger The control switch of pump.
Further, the present apparatus further includes fixed lid, and the fixed lid is fixed on the type chamber aperture position in fixing seat, Compress the simultaneously intracavitary liquid feeding pin group of fixed.
Further, the present apparatus further includes connecting plate, and the fixing seat is connected to the side of connecting plate, the connecting plate The other side connects work arm.
Preferably, the liquid feeding pin group is eight liquid feeding pins.
Preferably, the control switch is several solenoid valves.
The present apparatus mainly has the advantage that
Whole quantitative liquid feeding avoids manual operation bring error, realizes higher extraction efficiency.
Micro liquid feeding blending system draws 20-150ul liquid every time, and different liquid needed for mixing well experiment are kept away Cross contamination when artificial liquid feeding is exempted from.
The degree of automation is higher, does not need manual operation, and after setting nucleic acid extraction program, instrument autonomous operation is avoided The fault that manual operation may cause.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
In figure: 1 is plunger pump, and 2 be connecting plate, and 3 be fixed lid, and 4 be liquid feeding pin group, and 5 be fixing seat, and 6 be bolt, and 7 are Hose.
Specific embodiment
As shown in Figure 1, the instrument for extracting nucleic acid of the utility model is with continuously liquid device is connect, mainly by plunger pump 1, controller, Hose 7, liquid feeding pin group 4, solenoid valve, connecting plate 2 and fixing seat 3 form, and wherein plunger pump 1 is multiple feed liquor mouth structures, each Inlet is connected respectively in different liquid tubes, and the outlet of plunger pump 1 is connected to liquid feeding pin group 4 by hose 7.Plunger pump 1 each inlet and liquid outlet passes through solenoid valve control, and solenoid valve is connected with the built-in controller in plunger pump 1.
Liquid feeding pin group 4 is eight liquid feeding pins, and eight liquid feeding pins are uniformly distributed and interconnect, be equipped in fixing seat 5 with The compatible type chamber of liquid feeding pin group 4, the type that fixing seat 5 is arranged in liquid feeding pin group 4 is intracavitary, and the syringe needle of liquid feeding pin passes through fixing seat 5, Fixed lid 3 is equipped with above the type chamber of fixing seat 3, fixed lid 3 is affixed in fixing seat 5 by bolt 6, is compressed and fixed The intracavitary liquid feeding pin group 4 of 5 types of seat.
Meanwhile fixing seat 5 is fastened on connecting plate 2, the other side of connecting plate 2 is connected to the work of instrument for extracting nucleic acid On mechanical arm, the mobile liquor charging of liquid feeding pin group 4 is controlled by mechanical arm.
In actual use, setting nucleic acid extraction program, device autonomous operation realize nucleic acid to the utility model first Extraction work, avoid realizing higher extraction efficiency due to manually just doing bring error.
During liquid feeding, by process control, the liquid of the extraction needed is determined, and opened by controller corresponding Electromagnetic valve switch draws corresponding liquid, draws 20-150uL every time, and the liquid of absorption flows through soft from the liquid outlet of plunger pump 1 Pipe 7 is sent into liquid feeding pin group 4, and is sent into test tube by liquid feeding pin group 4, until reaching required amount.Then controller root According to process control, switching solenoid valve switch replaces the liquid of absorption, and repeats above-mentioned suction operation, using micro multiple Method mixes well the amount of different liquids needed for experiment, and avoiding artificial liquid feeding is to generate cross contamination, influences experiment effect.
Above-described specific embodiment has carried out further the purpose of the present invention, technical scheme and beneficial effects It is described in detail, it should be understood that the foregoing is merely a specific embodiment of the invention, the guarantor that is not intended to limit the present invention Range is protected, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should all be contained in this hair Within bright protection scope.

Claims (5)

1. a kind of instrument for extracting nucleic acid continuous liquid adding device, connect with work arm, it is characterised in that: including plunger pump (1), Controller, hose (7), liquid feeding pin group (4) and fixing seat (5), the liquid feeding pin group (4) are that several liquid feeding pins are uniformly distributed simultaneously It interconnects, the fixing seat (5) is equipped with liquid feeding pin group (4) compatible type chamber, and liquid feeding pin group (4) setting is solid The type of reservation (5) is intracavitary, and the syringe needle of liquid feeding pin passes through fixing seat (5), and the plunger pump (1) is equipped with multiple inlets and one goes out Liquid mouth, each inlet are connected respectively to different liquid lines, and liquid outlet is connect with liquid feeding pin group (4) by hose (7), described Controller connects the control switch of plunger pump (1).
2. instrument for extracting nucleic acid continuous liquid adding device according to claim 1, it is characterised in that: it further include fixed lid (3), The fixed fixed lid (3) of type chamber aperture position on the fixing seat (5), compresses the simultaneously intracavitary liquid feeding pin group (4) of fixed.
3. instrument for extracting nucleic acid continuous liquid adding device according to claim 1, it is characterised in that: it further include connecting plate (2), The fixing seat (5) is connected to the side of connecting plate (2), and the other side of the connecting plate (2) connects work arm.
4. instrument for extracting nucleic acid continuous liquid adding device according to claim 1, it is characterised in that: the liquid feeding pin group (4) For eight liquid feeding pins.
5. instrument for extracting nucleic acid continuous liquid adding device according to claim 1, it is characterised in that: if the control switch is Dry solenoid valve.
CN201820758201.1U 2018-05-21 2018-05-21 A kind of instrument for extracting nucleic acid continuous liquid adding device Active CN208815031U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820758201.1U CN208815031U (en) 2018-05-21 2018-05-21 A kind of instrument for extracting nucleic acid continuous liquid adding device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820758201.1U CN208815031U (en) 2018-05-21 2018-05-21 A kind of instrument for extracting nucleic acid continuous liquid adding device

Publications (1)

Publication Number Publication Date
CN208815031U true CN208815031U (en) 2019-05-03

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CN201820758201.1U Active CN208815031U (en) 2018-05-21 2018-05-21 A kind of instrument for extracting nucleic acid continuous liquid adding device

Country Status (1)

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CN (1) CN208815031U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110229751A (en) * 2019-07-17 2019-09-13 中国人民解放军军事科学院军事医学研究院 MRNA preparation system and preparation method
CN112011533A (en) * 2019-05-13 2020-12-01 德诺杰亿(北京)生物科技有限公司 Integrated system for extracting and detecting nucleic acid and detection method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112011533A (en) * 2019-05-13 2020-12-01 德诺杰亿(北京)生物科技有限公司 Integrated system for extracting and detecting nucleic acid and detection method
CN112011533B (en) * 2019-05-13 2024-07-23 德诺杰亿(北京)生物科技有限公司 Integrated system for extracting and detecting nucleic acid and detection method
CN110229751A (en) * 2019-07-17 2019-09-13 中国人民解放军军事科学院军事医学研究院 MRNA preparation system and preparation method

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Effective date of registration: 20211104

Address after: 215522 No. 8, Shengyu Avenue, Haiyu Town, Changshu City, Suzhou City, Jiangsu Province

Patentee after: SUZHOU ENRICHING BIOTECHNOLOGY CO.,LTD.

Address before: 211100 No.18 Zhilan Road, gaoxinyuan, Jiangning District, Nanjing City, Jiangsu Province

Patentee before: NANJING ENRICHING MAIGE BIOCHEMISTRY APPARATUS CO.,LTD.

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