CN212347796U - Chromatography device - Google Patents
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- CN212347796U CN212347796U CN202020797942.8U CN202020797942U CN212347796U CN 212347796 U CN212347796 U CN 212347796U CN 202020797942 U CN202020797942 U CN 202020797942U CN 212347796 U CN212347796 U CN 212347796U
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Abstract
本实用新型公开了一种层析装置。该层析装置包括样品在线调节机构、样品储存机构、气泡陷阱机构以及层析机构，样品在线调节机构包括样品调节罐、搅拌组件、酸液进管、碱液进管、电导调节液进管、pH感应器以及电导感应器，样品调节罐上设置有样品进样口，酸液进管、碱液进管以及电导调节液进管均与样品调节罐连通，pH感应器以及电导感应器均与所述样品调节罐连接，搅拌组件与所述样品调节罐连接以实现对所述样品调节罐内的液体搅拌，所述样品储存机构、所述气泡陷阱机构以及所述层析机构依次顺序与所述样品调节罐连通，所述样品储存机构与所述气泡陷阱机构之间设置有层析缓冲液接口。该层析装置能够节时节力、提高层析效率、稳定性高。
The utility model discloses a chromatography device. The chromatography device includes a sample online adjustment mechanism, a sample storage mechanism, a bubble trap mechanism and a chromatography mechanism. The sample online adjustment mechanism includes a sample adjustment tank, a stirring assembly, an acid liquid inlet pipe, an alkali liquid inlet pipe, a conductivity adjustment liquid inlet pipe, The pH sensor and the conductivity sensor, the sample adjustment tank is provided with a sample inlet, the acid liquid inlet pipe, the alkali liquid inlet pipe and the conductivity adjustment liquid inlet pipe are all connected with the sample adjustment tank, and the pH sensor and the conductivity sensor are connected with the sample adjustment tank. The sample conditioning tank is connected, and the stirring component is connected with the sample conditioning tank to realize agitation of the liquid in the sample conditioning tank. The sample storage mechanism, the bubble trap mechanism and the chromatography mechanism are sequentially connected with each The sample adjustment tank is communicated, and a chromatography buffer interface is arranged between the sample storage mechanism and the bubble trap mechanism. The chromatography device can save time and effort, improve the chromatography efficiency, and has high stability.
Description
Technical Field
The utility model relates to the field of biotechnology, especially, relate to a chromatographic device.
Background
Many samples need to be conditioned for pH and conductivity before chromatographic purification, and in the prior art, the conditioning process is generally manually performed. For example, the pH can be lowered or raised by adding an amount of acidic or basic solution to the sample, diluting the sample with a low salt solution to lower the conductivity of the sample, or adding a high salt solution to the sample to raise the conductivity of the sample, taking samples each time after adding an amount of conditioning solution to detect the pH and conductivity so as to prevent them from exceeding the target ranges, and then starting the chromatographic application after the conditioning of the sample is completed. The manual adjustment process is time consuming and labor consuming, and the pH and conductivity of the sample cannot be detected in real time, so that the risk of sample pollution is increased while the operation is performed. Such manual adjustment typically involves a dozen or hundreds of liters of sample at a time, and the loading process typically takes several hours, which increases the risk of sample damage if the sample is not stable under the pH and conductivity conditions required for loading. If a batch of samples is adjusted by dividing it into multiple batches, although reducing the exposure time of the samples reduces the risk of the samples being destroyed, this increases the number of manual operations and the amount of labour involved.
SUMMERY OF THE UTILITY MODEL
Accordingly, it is necessary to provide a chromatography apparatus which can save time and labor, improve chromatography efficiency, and has high stability.
A chromatography device, which comprises a sample on-line adjusting mechanism, a sample storage mechanism, a bubble trap mechanism and a chromatography mechanism, the sample on-line adjusting mechanism comprises a sample adjusting tank, a stirring component, an acid liquor inlet pipe, an alkali liquor inlet pipe, a conductivity adjusting liquid inlet pipe, a pH sensor and a conductivity sensor, the sample adjusting tank is provided with a sample injection port, the acid liquor inlet pipe, the alkali liquor inlet pipe and the electric conductance adjusting liquid inlet pipe are all communicated with the sample adjusting tank, the pH sensor and the conductivity sensor are both connected with the sample adjusting tank, the stirring component is connected with the sample adjusting tank to realize the stirring of the liquid in the sample adjusting tank, the sample storage mechanism, the bubble trap mechanism and the chromatography mechanism are sequentially communicated with the sample adjusting tank, and a chromatography buffer solution interface is also arranged between the sample storage mechanism and the bubble trap mechanism.
In one embodiment, the in-line sample adjustment mechanism further comprises a sample inlet tube, and the sample inlet tube is communicated with the sample inlet.
In one embodiment, the online sample adjustment mechanism further comprises a pneumatic valve, and the acid inlet pipe, and/or the alkali inlet pipe, and/or the conductivity adjustment inlet pipe, and/or the sample inlet pipe is provided with the pneumatic valve.
In one embodiment, the online sample adjusting mechanism further comprises a metering pump, and the metering pump is arranged on the acid liquid inlet pipe and/or the alkali liquid inlet pipe.
In one embodiment, the online sample adjustment mechanism further comprises a diaphragm pump, and the conductivity adjustment liquid inlet pipe and/or the sample inlet pipe is/are provided with the diaphragm pump.
In one embodiment, an acid liquid outlet is arranged at one end of the acid liquid inlet pipe close to the sample adjusting tank;
and/or one end of the alkali liquor inlet pipe close to the sample adjusting tank is provided with an alkali liquor outlet;
and/or one end of the conductance regulating liquid inlet pipe close to the sample regulating tank is provided with a conductance regulating liquid outlet;
and/or one end of the sample inlet pipe close to the sample adjusting tank is provided with a sample liquid outlet.
In one embodiment, the sample conditioning tank is provided with a conditioning tank vent, and the conditioning tank vent is provided with a pneumatic valve for opening or closing the conditioning tank vent.
In one embodiment, one or more of a bubble sensor, a pH sensor, a conductance sensor, a pressure sensor and a diaphragm pump are arranged on the pipeline between the sample storage mechanism and the bubble trap mechanism.
In one embodiment, the bubble trap mechanism is communicated with the sample storage mechanism and the chromatography mechanism through a pneumatic valve group with a bypass function, and a plurality of liquid level sensors are arranged on the bubble trap mechanism.
In one embodiment, the chromatography mechanism is communicated with the bubble trap mechanism through a pneumatic valve group with a bypass function.
In one embodiment, a flow meter and/or a pressure sensor are arranged on a pipeline between the bubble trap mechanism and the chromatography mechanism.
In one embodiment, the liquid outlet end of the chromatography mechanism is further provided with one or more of a UV detector, a pH sensor, a conductivity sensor and a multi-channel pneumatic valve set.
The utility model discloses a chromatography device is being used for the chromatography during operation, and festival reinforce, improvement chromatography efficiency, stability are high. The utility model provides a manual regulation's process is wasted time and energy among the conventional art, moreover because the problem of the pH of unable real-time detection sample and conductance, has reduced the risk of sample pollution, once adjusts tens of liters or tens of hundreds of liters sample when having avoided such manual regulation, practices thrift a large amount of time, and the sample is high at the required pH of appearance and the condition stability of conductance, reduces the destroyed risk of sample. The utility model discloses can realize a batch sample continuous operation, under the prerequisite that does not increase artificial operation number of times and amount of labour, can reach and reduce the sample exposure time and also reduce the purpose of the destroyed risk of sample.
The utility model discloses a chromatographic device is when using, the sample, the electric conductance regulator liquid, acidizing fluid and/or alkali lye fill up the pipeline that corresponds earlier, then the sample, the electric conductance regulator liquid, acidizing fluid and/or alkali lye get into the sample adjustment jar according to the proportion of preliminary testing, the low-speed stirring mixing, meanwhile pH inductor and electric conductance inductor real-time detection in the sample adjustment jar, the system program is according to the data control sample's of feedback add volume, the add volume of electric conductance regulator liquid, the add volume of acidizing fluid and/or the add volume of alkali lye finely tune, reach the preset control within range when pH and electric conductance, the sample adjustment stops. The sample adjusting tank is communicated with the sample storage mechanism, all adjusted samples enter the sample storage mechanism, the sample adjusting tank is disconnected from the sample storage mechanism, chromatography is started, sample loading is started, in the sample loading process, the sample adjusting tank starts to repeat the sample adjusting work, the adjusted samples enter the sample storage mechanism, the adjusted samples are kept in the sample storage mechanism all the time, meanwhile, the samples are adjusted in batches in the sample adjusting tank, online adjustment and sample loading of the samples are achieved, and the adjustment and sample loading are achieved until all the samples are adjusted.
Drawings
Fig. 1 is a schematic view of a chromatography apparatus according to an embodiment of the present invention.
Description of the reference numerals
10. A chromatographic device; 100. a sample online adjusting mechanism; 101. a sample conditioning tank; 1011. adjusting a tank vent; 102. stirring the slurry; 103. a sample inlet pipe; 1031. a sample drain port; 104. the electric conduction regulating liquid enters the pipe; 1041. a conductivity regulating liquid outlet; 105. feeding acid liquor into the pipe; 1051. an acid liquor drain port; 106a, 106b, 106c, a pH sensor; 107a, 107b, 107c, conductance sensors; 108. feeding alkali liquor into the pipe; 1081. an alkali liquor outlet; 109a, 109b, 109c, 109d, 109e, 109f, pneumatic valves; 110a, 110b, 110c, diaphragm pumps; 111a, 111b, 111c, 111d, 111e, flow meter; 112a, 112b, a metering pump; 200. a sample storage mechanism; 201. a storage vent; 300. a bubble trap mechanism; 301. an exhaust port of the trap mechanism; 302. a liquid discharge port of the trap mechanism; 400. a chromatography mechanism; 500a, 500b, a multi-channel pneumatic valve bank; 600a, 600b, a pressure sensor; 700. a UV detector; 800a and 800b, a pneumatic valve group with a bypass function; 900a, 900b, a liquid level sensor; 1001. a chromatography buffer interface; 1002. a liquid outlet; 1100. a bubble sensor.
Detailed Description
In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. The preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
In the description of the present invention, it should be understood that the terms used in the present invention are used in the description of the present invention, and it should be understood that the terms "center", "upper", "lower", "bottom", "inner", "outer" and the like used in the present invention are used as the terms of the orientation or the positional relationship shown in the drawings, and are only used for convenience of description and simplification of the description, but do not indicate or imply that the device or the element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be interpreted as limiting the present invention.
It should be understood that the terms "first", "second", etc. are used herein to describe various information, but the information should not be limited to these terms, and these terms are only used to distinguish one type of information from another. For example, "first" information may also be referred to as "second" information, and similarly, "second" information may also be referred to as "first" information, without departing from the scope of the present invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening elements, or they may be in communication within two elements, i.e., when an element is referred to as being "secured to" another element, it may be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1, one embodiment of the present invention provides a chromatography apparatus 10.
The chromatography device 10 includes a sample online adjustment mechanism 100, a sample storage mechanism 200, a bubble trap mechanism 300, a chromatography mechanism 400, and a control mechanism. The control mechanism, which may be a PLC, a PID, etc., is electrically connected to the sample online adjustment mechanism 100, the bubble trap mechanism 300, and the chromatography mechanism 400, and is not shown in fig. 1.
The sample online adjustment mechanism 100 comprises a sample adjustment tank 101, a stirring assembly 102, an acid inlet pipe 105, an alkali inlet pipe 108, a conductivity adjustment inlet pipe 104, a pH sensor 106a and a conductivity sensor 107 a. The sample adjusting tank 101 is provided with a sample inlet. The acid liquid inlet pipe 105, the alkali liquid inlet pipe 108 and the electric conduction regulating liquid inlet pipe 104 are all communicated with the sample regulating tank 101. The pH sensor 106a and the conductance sensor 107a are connected to the sample-adjusting tank 101. The stirring assembly 102, the pH sensor 106a and the conductivity sensor 107a are electrically connected to a control mechanism, and the control mechanism controls the addition amount of the sample, the addition amount of the conductivity adjusting liquid, the addition amount of the acid liquid and/or the addition amount of the alkali liquid according to data fed back by the pH sensor 106a and the conductivity sensor 107a for fine adjustment.
Further, a sample inlet pipe 103 is connected to the sample inlet.
The acid inlet pipe 105 is provided with a pneumatic valve 109c, the alkali inlet pipe 108 is provided with a pneumatic valve 109f, and/or the conductivity adjusting liquid inlet pipe 104 is provided with a pneumatic valve 109b, and/or the sample inlet pipe 103 is provided with a pneumatic valve 109 a. Pneumatic valves 109a, 109b, 109c, 109f may each be electrically connected to a control mechanism.
In one embodiment, the chromatography device 10 further comprises a flow meter, wherein the flow meter 111a is disposed on the sample inlet pipe 103, and the flow rate of the sample inlet pipe 103 is monitored and controlled by the flow meter 111 a. And/or the conductance regulating liquid inlet pipe 104 is provided with a flow meter 111b, and the flow rate of the conductance regulating liquid inlet pipe 104 is monitored and controlled by the flow meter 111 b. And/or the acid liquor inlet pipe 105 is also provided with a flow meter 111c, and the flow of the acid liquor inlet pipe 105 is monitored and controlled through the flow meter 111 c. And/or a flow meter 111e is arranged on the alkali liquor inlet pipe 108, and the flow of the alkali liquor inlet pipe 108 is monitored and controlled through the flow meter 111 e. The flow meters 111a, 111b, 111c, 111e are each electrically connected to the control mechanism.
An agitation assembly 102 is coupled to the sample conditioning tank 101 to effect agitation of the liquid within the sample conditioning tank 101. Further, the stirring assembly 102 includes a stirring paddle and a stirring motor electrically connected to the control mechanism. The stirring rake sets up in sample regulation jar 101, and agitator motor installs and just is connected with the stirring rake at the top of sample regulation jar 101, and agitator motor is used for driving the stirring rake to rotate, and agitator motor's rotational speed is adjustable, controllable to make the rotational speed that can the stirring rake adjustable, controllable. The paddles and agitator motor are not shown in FIG. 1.
The sample storage mechanism 200, the bubble trap mechanism 300, and the chromatography mechanism 400 are sequentially communicated with the sample-conditioning tank 101. Specifically, the sample storage mechanism 200 communicates with the sample adjustment tank 101, and the sample storage mechanism 200 communicates with the bubble trap mechanism 300 and the chromatography mechanism 400 in this order through a pipe.
The sample storage mechanism 200 may be a sample storage tank. When setting up, the position setting of sample storage mechanism 200 is in the below of sample regulation jar 101, so set up, can make the good sample of regulation get into in sample storage mechanism 200 under the action of gravity, need not the mechanical pump, practices thrift electric energy resource. Pneumatic valve 109d is provided on the conduit between sample conditioning tank 101 and sample storage mechanism 200.
A chromatography buffer interface 1001 is arranged between the sample storage mechanism 200 and the bubble trap mechanism 300, and the chromatography buffer interface 1001 is used for feeding a chromatography buffer. In one embodiment, there are multiple chromatography buffer interfaces 1001, and multiple chromatography buffer interfaces 1001 communicate with the tubing between the sample storage mechanism 200 and the bubble trap mechanism 300 through the multi-channel pneumatic valve set 500 a.
Furthermore, an external liquid inlet mechanism can be arranged at the interface of the chromatography buffer solution. Specifically, the liquid inlet mechanism communicates with the conduit between the sample storage mechanism 200 and the bubble trap mechanism 300 through the multi-channel pneumatic valve block 500 a. The multi-channel pneumatic valve set 500a is communicated with the liquid inlet mechanism through a plurality of chromatography buffer liquid interfaces 1001. The liquid inlet mechanism is used for pumping chromatography buffer solution into the chromatography buffer solution interface 1001 and the chromatography mechanism 400, and can be a syringe, a sample inlet bottle, a sample inlet pump communicated with the chromatography buffer solution and the like. In fig. 1, the liquid inlet mechanism is not shown. The liquid feeding mechanism may be a third-party environment component, or may be a part of the chromatography device 10 of the present invention.
The bubble trap mechanism 300 has a trap mechanism exhaust port 301 and a trap mechanism drain port 302.
The utility model discloses a chromatography device 10 is being used for the chromatography during operation, and festival reinforce, improvement chromatography efficiency, stability are high. The utility model provides a manual regulation's process is wasted time and energy among the conventional art, moreover because the problem of the pH of unable real-time detection sample and conductance, has reduced the risk of sample pollution, once adjusts tens of liters or tens of hundreds of liters sample when having avoided such manual regulation, practices thrift a large amount of time, and the sample is high at the required pH of appearance and the condition stability of conductance, reduces the destroyed risk of sample. The utility model discloses can also realize that a batch of sample can continuous operation, under the prerequisite that does not increase artificial operation number of times and amount of labour, can reach and reduce the purpose that the sample exposure time also reduced the destroyed risk of sample.
In a specific example, the in-line sample adjustment mechanism 100 further comprises a diaphragm pump. Wherein, the sample inlet pipe 103 is provided with a diaphragm pump 110a, and/or the conductance-adjusting liquid inlet pipe 104 is provided with a diaphragm pump 110 b. Both of the diaphragm pumps 110a, 110b may be electrically connected to the control mechanism.
In one embodiment, the acid inlet pipe 105 is provided with an acid drain 1051 near one end of the sample conditioning tank 101. The acid liquid drain 1051 is provided for the purpose of quickly confirming that the acid liquid inlet tube 105 is filled with acid liquid by filling the acid liquid inlet tube 105 with acid liquid and draining a small portion through the acid liquid drain 1051 before chromatography is started. After the acid liquor inlet pipe 105 is filled with acid liquor, the adjustment accuracy can be improved, and the adjustment efficiency is improved.
And/or, the end of the alkali liquor inlet pipe 108 close to the sample adjusting tank 101 is provided with an alkali liquor outlet 1081. The purpose of the setting of the lye discharge port 1081 is to quickly confirm that the lye inlet pipe 108 is filled with lye by filling the lye inlet pipe 108 with lye and discharging a small portion through the lye discharge port 1081 before chromatography begins. After the alkali liquor inlet pipe 108 is filled with the alkali liquor, the adjustment accuracy can be improved, and the adjustment efficiency is improved.
And/or one end of the conductivity adjusting liquid inlet pipe 104 close to the sample adjusting tank 101 is provided with a conductivity adjusting liquid outlet 1041. The purpose of the setting of the conductivity adjusting liquid drain hole 1041 is to fill the conductivity adjusting liquid inlet pipe 104 with the conductivity adjusting liquid before the start of chromatography, and to discharge a small part through the conductivity adjusting liquid drain hole 1041, so that it can be quickly confirmed that the conductivity adjusting liquid inlet pipe 104 is filled with the conductivity adjusting liquid. After the conductance regulator liquid inlet pipe 104 is filled with the conductance regulator liquid, the regulation precision can be improved, and the regulation efficiency can be improved.
And/or, a sample liquid outlet 1031 is arranged at one end of the sample inlet pipe 103 close to the sample adjustment tank 101. The purpose of the sample drain port 1031 is to quickly confirm that the sample inlet pipe 103 is filled with the sample by filling the sample inlet pipe 103 with the conductivity adjusting liquid and draining a small portion through the sample drain port 1031 before chromatography is started. After the sample inlet pipe 103 is filled with the sample, the adjustment accuracy can be improved, and the adjustment efficiency is improved.
In one specific example, the sample conditioning tank 101 is provided with a conditioning tank vent 1011. A pneumatic valve 109e for opening or closing the adjustment tank exhaust port 1011 is provided at the adjustment tank exhaust port 1011. The sample storage mechanism 200 is provided with a storage vent 201.
In a specific example, the in-line sample adjustment mechanism 100 further comprises a metering pump, which may be a high precision metering pump. The acid inlet pipe 105 is provided with a metering pump 112 a. And/or the alkali liquor inlet pipe 108 is provided with a metering pump 112 b. Both the metering pumps 112a and 112b can be electrically connected to the control mechanism. The metering pumps 112a, 112b can precisely control the amount of the acid solution or the alkali solution to be added, and the adjustment precision and the adjustment efficiency can be improved.
In a specific example, one or more of the bubble sensor 1100, the pressure sensor 600a, and the diaphragm pump 110c are disposed on a pipe between the sample storage mechanism 200 and the bubble trap mechanism 300. The bubble sensor 1100, the pressure sensor 600a and the diaphragm pump 110c are all electrically connected to the control mechanism. Preferably, a bubble sensor 1100, a pressure sensor 600a, and a diaphragm pump 110c are provided on a pipe between the sample storage mechanism 200 and the bubble trap mechanism 300. The positions of the bubble sensor 1100, the pressure sensor 600a, and the diaphragm pump 110c may be adjusted as needed. Furthermore, one or more of a pH sensor 106b and a conductance sensor 107b are disposed on the pipeline between the sample storage mechanism 200 and the bubble trap mechanism 300, and both the pH sensor 106b and the conductance sensor 107b can be electrically connected to the control mechanism. Preferably, a pH sensor 106b and a conductance sensor 107b are provided on the pipe between the sample storage mechanism 200 and the bubble trap mechanism 300.
A flow meter 111c is further provided on the pipe between the sample storage mechanism 200 and the bubble trap mechanism 300, and the flow rate on the pipe is monitored and controlled by the flow meter 111 c. The flow meter 111c may be electrically connected to the control mechanism.
In one embodiment, the bubble trap mechanism 300 communicates with the sample storage mechanism 200 and the chromatographic mechanism 400 through a pneumatic valve block with bypass function 800 a. The bubble trap mechanism 300 is provided with a plurality of liquid level sensors, at least one of which is disposed at the top of the bubble trap mechanism 300 and at least one of which is disposed at the bottom of the bubble trap mechanism 300. Preferably, referring to fig. 1, an embodiment of the present invention shows two liquid level sensors 900a and 900b, wherein one liquid level sensor 900a is disposed at the top of the bubble trap mechanism 300 and one liquid level sensor 900b is disposed at the bottom of the bubble trap mechanism 300. The pneumatic valve set 800a with bypass function and the liquid level sensors 900a and 900a can be electrically connected with the control mechanism.
A pressure sensor 600b may be provided on the conduit between the bubble trap mechanism 300 and the tomographic mechanism 400. The flow meter 111d may be further provided on the pipe between the bubble trap mechanism 300 and the tomographic mechanism 400, and the flow rate on the pipe is monitored and controlled by the flow meter 111 d. The pressure sensor 600b and the flow meter 111d can be electrically connected to the control mechanism.
In a specific example, the chromatography mechanism 400 communicates with the bubble trap mechanism 300 through a pneumatic valve block with bypass function 800 b. The pneumatic valve set with bypass function 800b can be electrically connected to the control mechanism.
In a specific example, the outlet end of the chromatography mechanism 400 is further communicated with a UV detector 700, and the UV detector 700 is used for detecting the protein concentration in the outlet. The UV detector 700 may be electrically connected to a control mechanism.
In a specific example, the liquid outlet port 1002 is further disposed at the liquid outlet end of the chromatography mechanism 400. The number of the liquid outlet ports 1002 may be plural. When the number of the liquid outlets 1002 is plural, the liquid outlet end is communicated with the plural liquid outlets 1002 through the multi-channel pneumatic valve set 500 b. The chromatography liquid after chromatography can flow out through any one of the liquid outlet ports 1002 through the multi-channel pneumatic valve set 500 b.
The conduit at the outlet end of the chromatographic mechanism 400 can also be provided with a conductivity sensor 107c and/or a pH sensor 106 c. The conductivity sensor 107c and the pH sensor 106c may be electrically connected to the control mechanism.
The utility model discloses a chromatography device 10 is with chromatography during operation, including following step:
opening pneumatic valves 109a, 109b, 109c and 109f on the sample inlet pipe 103, the conductivity adjusting liquid inlet pipe 104, the acid liquid inlet pipe 105 and the alkali liquid inlet pipe 108, filling the acid liquid inlet pipe 105 with acid liquid, discharging a small part through an acid liquid discharge port 1051, and quickly confirming that the acid liquid inlet pipe 105 is filled with acid liquid. Filling the alkali liquor inlet pipe 108 with alkali liquor, discharging a small part through an alkali liquor discharge port 1081, and quickly confirming that the alkali liquor inlet pipe 108 is filled with alkali liquor. The conductance regulator liquid inlet pipe 104 is filled with the conductance regulator liquid, and a small portion of the conductance regulator liquid is discharged through the conductance regulator liquid outlet 1041, so that it is quickly confirmed that the conductance regulator liquid inlet pipe 104 is filled with the conductance regulator liquid. The sample inlet pipe 103 is filled with the sample, and a small portion is discharged through the sample liquid outlet 1031, so that it is quickly confirmed that the sample inlet pipe 103 is filled with the sample.
The electric conduction regulating liquid, the acid liquid and/or the alkali liquid enter the sample regulating tank 101 according to a pre-tested proportion, and the stirring motor drives the stirring paddle to rotate at a low speed according to a preset rotating speed. The pH value and the conductance of the sample in the sample adjusting tank 101 are detected by a pH sensor 106a and a conductance sensor 107a, the pH value and the conductance data are fed back to a control mechanism in real time, the control mechanism compares preset values of the data, when the pH value and the conductance data exceed a preset control range, the adding amount of the sample, the adding amount of a conductance regulating solution, the adding amount of acid liquor and/or the adding amount of alkali liquor are finely adjusted, and a stirring motor keeps driving a stirring paddle to rotate at a low speed according to a preset rotating speed. The pH sensor 106a and the conductance sensor 107a in the sample adjustment tank 101 detect in real time, and when the pH and the conductance reach the preset control ranges, the sample adjustment is stopped.
The pneumatic valve 109d between the sample adjustment tank 101 and the sample storage mechanism 200 is opened, the sample in the sample adjustment tank 101 enters the sample storage mechanism 200 under the action of gravity, the adjusted sample completely enters the sample storage mechanism 200, and the pneumatic valve 109d between the sample adjustment tank 101 and the sample storage mechanism 200 is disconnected.
And (3) starting chromatography, wherein the liquid inlet mechanism starts to input the chromatography buffer solution to a pipeline between the sample storage mechanism 200 and the bubble trap mechanism 300 through the chromatography buffer solution interface 1001 and the multi-channel pneumatic valve group 500a, so that the chromatography buffer solution and the sample are mixed. The chromatography buffer solution and the sample are mixed to form a mixed solution, the mixed solution passes through the detection of the bubble sensor 1100, the pH sensor 106b, the conductivity sensor 107b and the pressure sensor 600a, the mixed solution reaches the bubble trap mechanism 300 after various detections, the bubble trap mechanism 300 removes bubbles from the mixed solution, and the mixed solution after the bubbles are removed passes through the pressure detection of the pressure sensor 600b again. In the process of sample loading, the sample adjusting tank 101 starts to repeat the sample adjusting work again, and the adjusted sample enters the sample storage mechanism 200 for storage, so that the adjusted sample is always kept in the sample storage mechanism 200 for sample loading, and meanwhile, batch adjusting samples are arranged in the sample adjusting tank 101.
The mixed liquid processed by the bubble trap mechanism 300 and subjected to pressure detection by the pressure sensor 600b enters the chromatography mechanism 400 to perform a chromatography process, and the flow rate of the mixed liquid entering the chromatography mechanism 400 can be monitored and controlled by the flow meter 111 d. The chromatography liquid after chromatography can be discharged through the multi-channel pneumatic valve set 500b after being detected by the UV detector 700, and the chromatography liquid after chromatography can flow out through any one or more liquid outlets 1002 through the multi-channel pneumatic valve set 500 b.
To sum up, the utility model discloses a chromatographic device 10 is when using, the sample, the electric conductance regulator liquid, the pipeline that corresponds is filled with earlier to acidizing fluid and/or alkali lye, then the sample, the electric conductance regulator liquid, acidizing fluid and/or alkali lye get into sample conditioning jar 101 according to the proportion of preliminary testing, the low-speed stirring mixing, meanwhile pH inductor 106a and electric conductance inductor 107a real-time detection in the sample conditioning jar 101, control mechanism is according to the addition of the data control sample of feedback, the addition of electric conductance regulator liquid, the addition of acidizing fluid and/or the addition of alkali lye is finely tuned, pH and the electric conductance data that feed back when pH inductor 106a and electric conductance inductor 107a reach control mechanism and predetermine the within range, the sample adjustment stops. The sample conditioning tank 101 is in communication with the sample storage mechanism 200, the conditioned sample is all introduced into the sample storage mechanism 200, and the sample conditioning tank 101 is disconnected from the sample storage mechanism 200. The chromatography is started, the liquid inlet mechanism and the chromatography mechanism 400 work, the liquid inlet mechanism starts to pump chromatography buffer liquid to be mixed with a sample and then enters the chromatography mechanism 400, in the working process of the chromatography mechanism, the sample adjusting tank 101 starts to repeat the sample adjusting work, the adjusted sample enters the sample storage mechanism 200, the sample storage mechanism 200 always keeps the adjusted sample for sample loading, and meanwhile, the sample is adjusted in the sample adjusting tank 101 in batches and at intervals, so that the online adjustment and sample loading of the sample are realized until all the samples are adjusted, the circulation of the adjustment and sample loading is realized, and the online continuous chromatography work is realized.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (12)
1. A chromatography device is characterized by comprising a sample on-line adjusting mechanism, a sample storage mechanism, a bubble trap mechanism and a chromatography mechanism, the sample on-line adjusting mechanism comprises a sample adjusting tank, a stirring component, an acid liquor inlet pipe, an alkali liquor inlet pipe, a conductivity adjusting liquid inlet pipe, a pH sensor and a conductivity sensor, the sample adjusting tank is provided with a sample injection port, the acid liquor inlet pipe, the alkali liquor inlet pipe and the electric conductance adjusting liquid inlet pipe are all communicated with the sample adjusting tank, the pH sensor and the conductivity sensor are both connected with the sample adjusting tank, the stirring component is connected with the sample adjusting tank to realize the stirring of the liquid in the sample adjusting tank, the sample storage mechanism, the bubble trap mechanism and the chromatography mechanism are sequentially communicated with the sample adjusting tank, and a chromatography buffer solution interface is also arranged between the sample storage mechanism and the bubble trap mechanism.
2. The chromatography device of claim 1 wherein said in-line sample adjustment mechanism further comprises a sample inlet tube, said sample inlet tube communicating with said sample inlet port.
3. The chromatography device according to claim 2, wherein said in-line sample adjustment mechanism further comprises a pneumatic valve, said acid inlet, and/or said alkaline inlet, and/or said conductivity-adjusting inlet, and/or said sample inlet is provided with said pneumatic valve.
4. The chromatography device according to claim 2, wherein the online sample adjustment mechanism further comprises a metering pump, and the metering pump is arranged on the acid liquid inlet pipe and/or the alkali liquid inlet pipe.
5. The chromatography device according to claim 2, wherein the sample in-line adjusting mechanism further comprises a diaphragm pump, and the conductivity adjusting liquid inlet pipe and/or the sample inlet pipe are provided with the diaphragm pump.
6. The chromatography device according to claim 2, wherein an acid liquid outlet is arranged at one end of the acid liquid inlet pipe close to the sample adjusting tank;
and/or one end of the alkali liquor inlet pipe close to the sample adjusting tank is provided with an alkali liquor outlet;
and/or one end of the conductance regulating liquid inlet pipe close to the sample regulating tank is provided with a conductance regulating liquid outlet;
and/or one end of the sample inlet pipe close to the sample adjusting tank is provided with a sample liquid outlet.
7. The chromatography device according to claim 1, wherein the sample conditioning tank is provided with a conditioning tank vent, and the conditioning tank vent is provided with a pneumatic valve for opening or closing the conditioning tank vent.
8. The chromatography device according to any one of claims 1 to 7, wherein one or more of a bubble sensor, a pH sensor, a conductivity sensor, a pressure sensor and a diaphragm pump is arranged on a pipeline between the sample storage mechanism and the bubble trap mechanism.
9. The chromatography device according to any one of claims 1 to 7, wherein the bubble trap mechanism is communicated with the sample storage mechanism and the chromatography mechanism through a pneumatic valve set with a bypass function, and a plurality of liquid level sensors are arranged on the bubble trap mechanism.
10. The chromatography device according to any one of claims 1 to 7, wherein the chromatography mechanism communicates with the bubble trap mechanism through a pneumatic valve bank with a bypass function.
11. The chromatography device according to any one of claims 1 to 7, wherein a flow meter and/or a pressure sensor is provided on the conduit between the bubble trap mechanism and the chromatography mechanism.
12. The chromatography device according to any one of claims 1 to 7, wherein the liquid outlet end of the chromatography mechanism is further provided with one or more of a UV detector, a pH sensor, a conductivity sensor and a multi-channel pneumatic valve set.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020797942.8U CN212347796U (en) | 2020-05-14 | 2020-05-14 | Chromatography device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020797942.8U CN212347796U (en) | 2020-05-14 | 2020-05-14 | Chromatography device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113663364A (en) * | 2020-05-14 | 2021-11-19 | 佛山汉腾生物科技有限公司 | Chromatography device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113663364A (en) * | 2020-05-14 | 2021-11-19 | 佛山汉腾生物科技有限公司 | Chromatography device |
| CN113663364B (en) * | 2020-05-14 | 2025-02-21 | 佛山汉腾生物科技有限公司 | Chromatography device |
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