CN110531100A - A kind of liquid feeding method - Google Patents
A kind of liquid feeding method Download PDFInfo
- Publication number
- CN110531100A CN110531100A CN201910822284.5A CN201910822284A CN110531100A CN 110531100 A CN110531100 A CN 110531100A CN 201910822284 A CN201910822284 A CN 201910822284A CN 110531100 A CN110531100 A CN 110531100A
- Authority
- CN
- China
- Prior art keywords
- reagent
- outlet tube
- syringe pump
- pure water
- bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention proposes a kind of liquid feeding methods, it can be while processing before batch samples carry out, it solves the problem of to lead to liquid feeding error problem by outlet tube residual liquid and had not only prevented two kinds or more of reagent cross contaminations in the case where guaranteeing efficient situation but also saved reagent.
Description
Technical field
The present invention is a kind of liquid feeding method, belongs to measurement the field of test technology.
Background technique
In the experiment such as all kinds of chemistry, biology, most laboratory samples will pass through pre-treatment, and Sample Pretreatment Technique
Develop slowly and constrain the development of analytical chemistry.With the continuous increasing of the complexity of the property and step of present sample
Add, it is elongated so as to cause the test period.Meanwhile current sample pretreatment device is mainly that syringe pump is allocated liquid, injection
Reagent can remain in pipeline inner wall when pump distribution liquid, eventually result in result and generate deviation.
In addition, needing to be added variety classes reagent in processing before batch samples carry out, often plus a kind of reagent injector is pumped
Inlet tube, outlet tube and the injection needle for just needing rinse one injection to pump, then will increase the workload of syringe pump, cause to imitate
Rate reduces；And reagent is wasted, resulting in waste of resources leads to increased costs.
Summary of the invention
In order to solve the above problem, the invention proposes a kind of liquid feeding methods, and the purpose is to can carry out in batch samples
While pre-treatment, solve to lead to liquid feeding error problem by outlet tube residual liquid, and both prevented in the case where guaranteeing efficient situation
Only the problem of reagent, is saved in two kinds or more of reagent cross contaminations again.
The purpose of the present invention is what is realized by technical solution below:
The step of this kind of liquid feeding method, is as follows:
Step 1: preparing
Vessel used include mother liquid multi-cavity bottle 1, sample bottle 2, waste liquid cup 3, pure water reagent bottle 4 and syringe pump 5, institute in this method
It states installation Y-valve 6, Y-valve 24 on syringe pump 5 and is separately connected inlet tube 8 and outlet tube 9 and injection needle 15；
Step 2: pure water cleans
Inlet tube 8 is placed in pure water reagent bottle 4, outlet tube 9 is placed on 3 top of waste liquid cup, and syringe pump 5 passes through feed liquor
Pipe 8 extracts pure water from pure water reagent bottle 4, passes sequentially through switching Y-valve 24 and goes out liquid direction to inlet tube 8, outlet tube 9 and injection
Needle tubing 15 is cleaned；
Step 3: air cleaning
Inlet tube 8 is placed in air or in empty reagent bottle, syringe pump 5 extracts air by inlet tube 8, successively leads to
Switching 24 outgassing direction of Y-valve is crossed to empty the residual pure water in inlet tube 8, outlet tube 9 and injection needle 15；
Step 4: adding a kind of reagent
Air is extracted by outlet tube 9 with syringe pump 5, then outlet tube 9 is inserted into mother liquid multi-cavity bottle 1 and extracts aequum reagent,
Then with syringe pump 5 by injection needle 15 by outlet tube 9 air and reagent be injected into sample bottle 2；
Step 5: adding other reagents
Repeat to repeat the operation of step 4 Step 2: three operation and dry 9 outer wall of outlet tube, complete addition other
Reagent；
Step 6: terminating and cleaning
The operation Step 2: three is repeated, outlet tube 9 is then placed on 3 top of waste liquid cup.
In a kind of implementation, Step 3: adding reagent rinse step between four, which is syringe pump 5 by going out liquid
Pipe 9 is out of mother liquid multi-cavity bottle 1 and extracts reagent, then reagent in outlet tube 9 is expelled in waste liquid cup 3, step 3 is repeated, by liquid out
Remaining reagent is expelled in waste liquid cup 3 in pipe 9.
In a kind of implementation, the injection needle 15 on syringe pump 5 adapts to the needs of different capabilities, the capacity by replacement
Range be 100 microlitres~25 milliliters.
In a kind of implementation, extracting air capacity in the step 4 cannot be less than 200 microlitre.
In a kind of implementation, the number of the pure water cleaning is 1 to 3 times.
In a kind of implementation, the number of air cleaning is 2 to 3 times.
In a kind of implementation, the number of the reagent rinse is 1 to 3 times.
It is 200 microlitres~400 microlitres that amount of reagent is extracted in a kind of implementation, in the step 4.
Detailed description of the invention
Fig. 1 injecting pump structure schematic diagram
Fig. 2 syringe pump liquid feeding structural schematic diagram
The automatic device structural schematic diagram of Fig. 3 syringe pump liquid feeding
Specific embodiment
Technical solution of the present invention is further described below with reference to drawings and examples:
As described in Figure 3, this kind of liquid feeding method apply the automatic device of syringe pump include syringe pump 5, wipe-drying device 6, into
Liquid pipe 8, outlet tube 9, injection needle 15, three-dimensional movement platform, C axis 11, workbench 17, peristaltic pump 22 and control system
12, the three-dimensional movement platform includes x-axis 13, Y-axis and Z axis, the outlet tube 9 is fixed in X-axis 13, the inlet tube
8 are fixed on C axis 11, and the control system 12 controls the three-dimensional movement platform 10 and the outlet tube 9 is driven to do upper and lower, left and right
Mobile, the control system 12 controls the C axis 11 and the inlet tube 8 is driven to move up and down, workbench 17 described in Fig. 2
It is the platform of a 4*5, number and the position of various sizes of mother liquid multi-cavity bottle and sample bottle can be arranged according to actual needs, it is described useless
Liquid cup 3 accounts for a position；Water-absorbent material, such as sponge are fixed with inside the top of two clamping limbs 20 of the wipe-drying device 6.
As described in Fig. 1,2, the step of this kind of liquid feeding method, is as follows:
1) prepare, mother liquid multi-cavity bottle 1, sample bottle 2 and waste liquid cup 3 are individually positioned on workbench 17, pure water reagent bottle 4 is put
It sets in 5 side of syringe pump, clear water reagent bottle 23 is placed on 22 side of peristaltic pump；Y-valve 24 is installed on the syringe pump 5, is passed through
The Y-valve 24 is separately connected inlet tube 8 and outlet tube 9 and injection needle 15；
2) outer wall is cleaned, the X-axis 13 is controlled by the control system 12, the outlet tube 9 is driven to be moved to described give up
3 top of liquid bottle, the peristaltic pump 22 extract clear water from clear water reagent bottle 23 and clean 9 outer wall of outlet tube, the then control
Two clamping limbs 20 folding that system 12 processed controls wipe-drying device 6 is dried the clear water of 9 outer wall of outlet tube by sponge；
3) pure water cleans, and the control system 12 controls the X-axis 13 and the outlet tube 9 is driven to be moved to the waste liquid cup
3 tops, the control system 12 control the C axis 11 and the inlet tube 8 are driven to be inserted into the pure water reagent bottle 4, the note
It penetrates pump 5 and pure water is extracted by the inlet tube 8, switch the Y-valve 24 to the outlet end 9, the injection needle 15 passes through
Pure water is discharged into the waste liquid cup 3 in the outlet tube 9, to the inlet tube 8, the outlet tube 9 and the injection needle
15 are cleaned；
4) air cleaning switches the Y-valve 24 to the liquid feeding end 8, drives inlet tube 8 upward by the C axis 11
Mobile, so that the inlet tube 8 leaves pure water reagent bottle 4, the syringe pump 5 extracts air by the inlet tube 8, switches institute
Y-valve 24 is stated to the outlet end 9, the syringe pump 5 is discharged air by the outlet tube 9, will remain in the outlet tube 9
Pure water empty；
5) add a kind of reagent, the Y-valve 24 rests on 9 end of outlet tube, by the X-axis 13 drive it is described go out
Liquid pipe 9 is moved in mother liquid multi-cavity bottle 1, and the syringe pump 5 extracts a certain amount of air by the outlet tube 9, then by 13 band of X-axis
It moves the outlet tube 9 and is inserted into extraction aequum reagent in the mother liquid multi-cavity bottle 1, then the X-axis 13 drives the outlet tube 9 mobile
Into the sample bottle 2, the syringe pump 5 empties the injection needle 15 by the syringe pump 5 will be in the outlet tube 9
Air and reagent are injected into the sample bottle 2,
And 5) 6) plurality of reagents is added, after the completion of a kind of addition of reagent, step 2), 3) 4) is repeated, until all reagents
Addition is completed；
And 4) 7) terminate and clean, repeat step 2), 3), the outlet tube 9 is moved to and rests on the waste liquid cup (3)
Top.
The step 3) and 4) between can add reagent rinse step, switch the Y-valve 24 to 9 end of outlet tube,
The outlet tube 9 is moved in the mother liquid multi-cavity bottle 1 and is extracted a certain amount of reagent, then the outlet tube 9 is moved to waste liquid
Reagent in the outlet tube 9, is expelled in the waste liquid cup 3 by the syringe pump 5, repeats step 3) by 3 top of cup, will
Remaining reagent is expelled in the waste liquid cup in the outlet tube 9, and the number of reagent rinse is 1 to 3 times.Rinse step is used for
When and data demand accuracy high to cleaning requirement is high, the rinse step only outlet tube described in rinse was both saved
Reagent prevents two kinds or more of reagent cross contaminations again.
Syringe pump 5 described in step 2) extract pure water to the inlet tube 8, outlet tube 9 and the injection needle 15 into
Row cleaning, effectively avoids cross-infection between reagent；Step 3) air cleaning can effectively empty residual pure water, avoid
Liquid feeding error；Outlet tube 9 described in step 4) first extracts extract after air needed for reagent, when liquid feeding the outlet tube 9 first plus institute
It needs reagent to be then exhausted from air, helps for reagent to be all discharged, a small amount of reagent has been avoided to remain in 9 inner wall of outlet tube.
Claims (8)
1. a kind of liquid feeding method, it is characterised in that: the step of this method is as follows:
Step 1: preparing
Vessel used include mother liquid multi-cavity bottle (1), sample bottle (2), waste liquid cup (3), pure water reagent bottle (4) and syringe pump in this method
(5), Y-valve (6) are installed on the syringe pump (5), Y-valve (24) is separately connected inlet tube (8) and outlet tube (9) and injection
Needle tubing (15)；
Step 2: pure water cleans
Inlet tube (8) is placed in pure water reagent bottle (4), outlet tube (9) is placed on above waste liquid cup (3), and syringe pump (5) is logical
It crosses inlet tube (8) and extracts pure water from pure water reagent bottle (4), pass sequentially through switching Y-valve (24) and go out liquid direction to inlet tube
(8), outlet tube (9) and injection needle (15) are cleaned；
Step 3: air cleaning
Inlet tube (8) are placed in air or in empty reagent bottle, syringe pump (5) extracts air by inlet tube (8), successively
The residual pure water in inlet tube (8), outlet tube (9) and injection needle (15) is emptied by switching Y-valve (24) outgassing direction；
Step 4: adding a kind of reagent
Air is extracted by outlet tube (9) with syringe pump (5), then aequum examination will be extracted in outlet tube (9) insertion mother liquid multi-cavity bottle (1)
Agent, then with syringe pump (5) by injection needle (15) by outlet tube (9) air and reagent be injected into sample bottle (2)；
Step 5: adding other reagents
It repeats to repeat the operation of step 4, complete other examinations of addition Step 2: three operation and dry outlet tube (9) outer wall
Agent；
Step 6: terminating and cleaning
The operation Step 2: three is repeated, then outlet tube (9) is placed on above waste liquid cup (3).
2. liquid feeding method according to claim 1, it is characterised in that: Step 3: between four add reagent rinse step,
The step is that syringe pump (5) pass through outlet tube (9) out of mother liquid multi-cavity bottle (1) and extract reagent, then the interior reagent of outlet tube (9) is discharged
In to waste liquid cup (3), step 3 is repeated, remaining reagent in outlet tube (9) is expelled in waste liquid cup (3).
3. liquid feeding method according to claim 1, it is characterised in that: the injection needle (15) on syringe pump (5) is by more
It changes to adapt to the needs of different capabilities, the range of the capacity is 100 microlitres~25 milliliters.
4. liquid feeding method according to claim 1, it is characterised in that: extracting air capacity in the step 4 cannot be less than
200 microlitres.
5. liquid feeding method according to claim 1, it is characterised in that: the number of the pure water cleaning is 1 to 3 times.
6. liquid feeding method according to claim 1, it is characterised in that: the number of air cleaning is 2 to 3 times.
7. liquid feeding method according to claim 2, it is characterised in that: the number of the reagent rinse is 1 to 3 times.
8. liquid feeding method according to claim 1, it is characterised in that: extracting amount of reagent in the step 4 is 200 microlitres
~400 microlitres.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910822284.5A CN110531100A (en) | 2019-08-30 | 2019-08-30 | A kind of liquid feeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910822284.5A CN110531100A (en) | 2019-08-30 | 2019-08-30 | A kind of liquid feeding method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110531100A true CN110531100A (en) | 2019-12-03 |
Family
ID=68666091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910822284.5A Pending CN110531100A (en) | 2019-08-30 | 2019-08-30 | A kind of liquid feeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110531100A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115684544A (en) * | 2022-10-25 | 2023-02-03 | 生态环境部南京环境科学研究所 | Integrated experimental device for various analysis of modified nano zero-valent iron |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110120213A1 (en) * | 2008-08-01 | 2011-05-26 | Shiseido Company, Ltd. | Sample injector, sample injecting method, and liquid chromatograph |
| CN203133096U (en) * | 2013-03-21 | 2013-08-14 | 浙江大学 | Full-automatic liquid sample collecting and feeding system without mixing and dead volume |
| CN106199032A (en) * | 2016-06-30 | 2016-12-07 | 南京润泽流体控制设备有限公司 | The sampling device of no cross contamination and control method thereof |
| CN106501539A (en) * | 2016-12-07 | 2017-03-15 | 苏州阿洛斯环境发生器有限公司 | A kind of sampling system and sample injection method for reducing dead volume liquid |
| CN109115579A (en) * | 2018-10-16 | 2019-01-01 | 湖北工业大学 | A kind of high throughput sample pre-treatments platform and liquid feeding method |
-
2019
- 2019-08-30 CN CN201910822284.5A patent/CN110531100A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110120213A1 (en) * | 2008-08-01 | 2011-05-26 | Shiseido Company, Ltd. | Sample injector, sample injecting method, and liquid chromatograph |
| CN203133096U (en) * | 2013-03-21 | 2013-08-14 | 浙江大学 | Full-automatic liquid sample collecting and feeding system without mixing and dead volume |
| CN106199032A (en) * | 2016-06-30 | 2016-12-07 | 南京润泽流体控制设备有限公司 | The sampling device of no cross contamination and control method thereof |
| CN106501539A (en) * | 2016-12-07 | 2017-03-15 | 苏州阿洛斯环境发生器有限公司 | A kind of sampling system and sample injection method for reducing dead volume liquid |
| CN109115579A (en) * | 2018-10-16 | 2019-01-01 | 湖北工业大学 | A kind of high throughput sample pre-treatments platform and liquid feeding method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115684544A (en) * | 2022-10-25 | 2023-02-03 | 生态环境部南京环境科学研究所 | Integrated experimental device for various analysis of modified nano zero-valent iron |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7939017B2 (en) | Automated fluid handling system and method | |
| EP2245468B1 (en) | Apparatus and method for cleaning a liquid handling probe | |
| US7621282B2 (en) | Probe washing cups and methods | |
| CN109115579A (en) | A kind of high throughput sample pre-treatments platform and liquid feeding method | |
| CN101869894B (en) | Clean device, purging system, cleaning method and particle analyzer | |
| CN109444450B (en) | Whole blood sample autoinjection device | |
| CN1894566B (en) | Method and apparatus for liquid chromatography automated sample loading | |
| US11401952B2 (en) | Waste liquid treatment apparatus, method and sample analyzer | |
| CN204034757U (en) | A kind of pipette Rapid Cleaning and get liquid servicing unit | |
| CN201974434U (en) | Automation device for fast cleaning sample needles | |
| CN106984600B (en) | Liquid transfer gun head automatic flushing device | |
| CN110531100A (en) | A kind of liquid feeding method | |
| JP7143414B2 (en) | sampling device | |
| CN203397138U (en) | Liquid channel control system of full-automatic enzyme immunoassay analyzer | |
| CN210875403U (en) | Full-automatic liquid feeding device | |
| CN109294890B (en) | Microbial fermentation multi-component online analysis device and use method thereof | |
| CN205099665U (en) | Automatic change board of extraction nucleic acid and cooperate cylinder of its use | |
| CN108614042B (en) | Automatic oil pumping device | |
| CN110658035A (en) | Liquid adding method applied to injection pump | |
| CN207611061U (en) | A kind of micro-fluidic chip sampling device based on over-pressure control | |
| CN201965136U (en) | Flow path system for automation biochemical reaction flow | |
| CN212964268U (en) | Automatic pretreatment device for samples | |
| CN214485727U (en) | Pressure extraction device for replacing Soxhlet extractor in material analysis | |
| CN109916817A (en) | A kind of liquid relief and analytical equipment | |
| CN113416636A (en) | Biological experiment operation equipment and use method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191203 |