CN110658035A - Liquid adding method applied to injection pump - Google Patents
Liquid adding method applied to injection pump Download PDFInfo
- Publication number
- CN110658035A CN110658035A CN201910821122.XA CN201910821122A CN110658035A CN 110658035 A CN110658035 A CN 110658035A CN 201910821122 A CN201910821122 A CN 201910821122A CN 110658035 A CN110658035 A CN 110658035A
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- China
- Prior art keywords
- outlet pipe
- liquid
- liquid outlet
- reagent
- injection pump
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
Abstract
The invention provides a liquid adding method applied to an injection pump, which can solve the problem of liquid adding error caused by liquid residual in a liquid outlet pipe while carrying out pretreatment on a large number of samples, and can prevent cross contamination of two or more reagents and save the reagents under the condition of ensuring high efficiency.
Description
Technical Field
The invention discloses a liquid adding method applied to an injection pump, and belongs to the technical field of measurement and testing.
Background
In various chemical and biological experiments, most of experimental samples need to be pretreated, and the development of the sample pretreatment technology slowly restricts the development of analytical chemistry. With the increasing nature of the samples and the complexity of the steps, the test period is longer. Meanwhile, the current sample pretreatment device mainly adopts an injection pump to dispense liquid, and reagents remain on the inner wall of a pipeline when the liquid is dispensed by the injection pump, so that the result is finally deviated.
In addition, when a large number of samples are pretreated, different reagents need to be added, and the liquid inlet pipe, the liquid outlet pipe and the injection needle pipe of the injection pump need to be rinsed once each reagent injection pump is added, so that the workload of the injection pump is increased, and the efficiency is reduced; and wastes reagents, resulting in resource waste and increased costs.
Disclosure of Invention
In order to solve the problems, the invention provides a liquid adding method applied to an injection pump, which can solve the problem of liquid adding error caused by liquid residual in a liquid outlet pipe while carrying out pretreatment on a large number of samples, and can prevent cross contamination of two or more reagents and save the reagents under the condition of ensuring high efficiency.
The purpose of the invention is realized by the following technical scheme:
the liquid adding method applied to the injection pump is characterized in that: the method comprises the following steps:
step one, preparation
The vessel used in the method comprises a mother liquid bottle 1, a sample bottle 2, a waste liquid cup 3, a pure water reagent bottle 4 and an injection pump 5, wherein a Y-shaped valve 6 is arranged on the injection pump 5, and a Y-shaped valve 24 is respectively connected with a liquid inlet pipe 8, a liquid outlet pipe 9 and an injection needle tube 15;
step two, pure water cleaning
Placing a liquid inlet pipe 8 in a pure water reagent bottle 4, placing a liquid outlet pipe 9 above a waste liquid cup 3, pumping pure water from the pure water reagent bottle 4 through the liquid inlet pipe 8 by an injection pump 5, and sequentially cleaning the liquid inlet pipe 8, the liquid outlet pipe 9 and an injection needle tube 15 by switching the liquid outlet direction of a Y-shaped valve 24;
step three, air cleaning
Placing the liquid inlet pipe 8 in air or an empty reagent bottle, pumping air by the injection pump 5 through the liquid inlet pipe 8, and sequentially switching the air outlet direction of the Y-shaped valve 24 to completely discharge residual pure water in the liquid inlet pipe 8, the liquid outlet pipe 9 and the injection needle tube 15;
step four, adding a reagent
Inserting a liquid outlet pipe 9 into a mother liquor bottle 1, pumping required amount of reagent by using an injection pump 5, then inserting the liquid outlet pipe 9 into a sample bottle 2, injecting the reagent in the liquid outlet pipe 9 into the sample bottle 2, then pumping air by using the injection pump 5 through a liquid inlet pipe 8 and injecting the air in the liquid inlet pipe 8 into the sample bottle 2 through the liquid outlet pipe 9 so as to discharge the residual reagent in the liquid outlet pipe 9;
step five, adding other reagents
Repeating the operation of the second step and the third step, wiping the outer wall of the liquid outlet pipe 9, and repeating the operation of the fourth step to finish adding other reagents;
step six, finishing and cleaning
And repeating the operation of the second step and the third step, and then placing the liquid outlet pipe 9 above the waste liquid cup 3.
In one implementation, the operation of step four is: after air is pumped through the liquid outlet pipe 9 by the injection pump 5, required amount of reagent is pumped from the mother liquid bottle 1, and then the air and the reagent in the liquid outlet pipe 9 are injected into the sample bottle 2.
In one implementation, a reagent rinsing step is added between the third step and the fourth step, in which the injection pump 5 pumps the reagent from the mother liquor bottle 1 through the liquid outlet pipe 9, then the reagent in the liquid outlet pipe 9 is discharged into the waste liquid cup 3, and the third step is repeated, so that the residual reagent in the liquid outlet pipe 9 is discharged into the waste liquid cup 3.
In one implementation, the syringe 15 on the syringe pump 5 is replaced to accommodate different volumes, ranging from 100 microliters to 25 milliliters.
In one implementation, the amount of air drawn in step four cannot be less than 600 microliters.
6. The priming method applied to the injection pump according to claim 2, characterized in that: the amount of extracted air cannot be less than 200 microliters.
In one implementation, the number of times of the pure water washing is 1 to 3 times.
In one implementation, the number of air purges is 2 to 3.
In one implementation, the number of rinses of the reagent is 1 to 3.
Drawings
FIG. 1 is a schematic view of a syringe pump
FIG. 2 is a schematic view of a liquid feeding structure of a syringe pump
FIG. 3 is a schematic view of an automatic device for filling liquid by a syringe pump
Detailed Description
The technical scheme of the invention is further detailed in the following by combining the drawings and the embodiment:
as shown in fig. 3, the automatic device for filling liquid by using the syringe pump in this embodiment includes a syringe pump 5, a wiping device 6, a liquid inlet tube 8, a liquid outlet tube 9, a syringe tube 15, a three-dimensional motion platform, a C-axis 11, a work platform 17, a peristaltic pump 22, and a control system 12, where the three-dimensional motion platform includes an X-axis 13, a Y-axis, and a Z-axis, the liquid outlet tube 9 is fixed on the X-axis 13, the liquid inlet tube 8 is fixed on the C-axis 11, the control system 12 controls the three-dimensional motion platform 10 to drive the liquid outlet tube 9 to move up and down and left and right, the control system 12 controls the C-axis 11 to drive the liquid inlet tube 8 to move up and down, the work platform 17 in fig. 2 is a 4 × 5 platform, the number and positions of mother liquid bottles and sample bottles with different sizes can be arranged according to actual needs, and the waste liquid cup 3 occupies; the top ends of the two holding arms 20 of the drying device 6 are internally fixed with water-absorbing materials, such as sponge.
As shown in fig. 1 and 2, the filling method applied to the syringe pump in this embodiment includes the following steps:
1) preparing, respectively placing a mother liquid bottle 1, a sample bottle 2 and a waste liquid cup 3 on a working platform 17, placing a pure water reagent bottle 4 on the side of an injection pump 5, and placing a clear water reagent bottle 23 on the side of a peristaltic pump 22; the injection pump 5 is provided with a Y-shaped valve 24, and the Y-shaped valve 24 is respectively connected with a liquid inlet pipe 8, a liquid outlet pipe 9 and an injection needle tube 15;
2) scrubbing the outer wall, controlling the X-axis 13 to drive the liquid outlet pipe 9 to move above the waste liquid bottle 3 through the control system 12, extracting clean water from a clean water reagent bottle 23 by the peristaltic pump 22 to clean the outer wall of the liquid outlet pipe 9, and then controlling the two clamping arms 20 of the scrubbing device 6 to be opened and closed by the control system 12 to scrub the clean water on the outer wall of the liquid outlet pipe 9 through the sponge;
3) pure water cleaning, the control system 12 controls the X-axis 13 to drive the liquid outlet pipe 9 to move above the waste liquid cup 3, the control system 12 controls the C-axis 11 to drive the liquid inlet pipe 8 to be inserted into the pure water reagent bottle 4, the injection pump 5 pumps pure water through the liquid inlet pipe 8, the Y-shaped valve 24 is switched to the liquid outlet end 9, the injection needle tube 15 discharges pure water through the liquid outlet pipe 9 into the waste liquid cup 3, and the liquid inlet pipe 8, the liquid outlet pipe 9 and the injection needle tube 15 are cleaned;
4) air cleaning, namely switching the Y-shaped valve 24 to the liquid inlet end 8, driving the liquid inlet pipe 8 to move upwards through the C shaft 11, so that the liquid inlet pipe 8 leaves the pure water reagent bottle 4, pumping air through the liquid inlet pipe 8 by the injection pump 5, switching the Y-shaped valve 24 to the liquid outlet end 9, and discharging air through the liquid outlet pipe 9 by the injection pump 5 to completely discharge residual pure water in the liquid outlet pipe 9;
5) adding a reagent, method one: the Y-shaped valve 24 stays at the end of the liquid outlet pipe 9, the liquid outlet pipe 9 is driven to move into the mother liquid bottle 1 through the X-axis 13, the injection pump 5 extracts a certain amount of required reagent through the liquid outlet pipe (9), then the liquid outlet pipe 9 is driven to move into the sample bottle (2) through the X-axis 13, and the reagent in the liquid outlet pipe 9 is injected into the sample bottle 2; switching the Y-shaped valve 24 to the end of the liquid inlet pipe 8, then pumping air through the liquid inlet pipe 8 by the injection pump 5, switching the Y-shaped valve 24 to the end of the liquid outlet pipe 9, discharging air through the liquid outlet pipe 9 by the injection pump 5, and discharging residual reagent in the liquid outlet pipe 9 to the sample bottle 2;
the second method comprises the following steps: the Y-shaped valve 24 stays at the end of the liquid outlet pipe 9, the liquid outlet pipe 9 is driven to move into the mother liquid bottle 1 through the X shaft 13, the injection pump 5 extracts a certain amount of air through the liquid outlet pipe 9, then the X shaft 13 drives the liquid outlet pipe (9) to be inserted into the mother liquid bottle 1 to extract a required amount of reagent, then the X shaft 13 drives the liquid outlet pipe 9 to move into the sample bottle 2, the injection pump 5 empties the injection needle tube 15 through the injection pump 5 to inject the air and the reagent in the liquid outlet pipe 9 into the sample bottle 2,
6) adding a plurality of reagents, and after one reagent is added, repeating the steps 2), 3), 4) and 5) until all the reagents are added;
7) finishing and cleaning, and repeating the steps 2), 3) and 4), wherein the liquid outlet pipe (9) moves to and stays above the waste liquid cup (3).
Step 3) and 4) between the added reagent rinse step, switch Y type valve 24 extremely 9 ends of drain pipe, will drain pipe 9 moves to extract a quantitative reagent in the mother liquor bottle 1, will again drain pipe 9 moves to waste liquid cup 3 top, will through injection pump 5 in the drain pipe 9 reagent discharge to in the waste liquid cup 3, repeat step 3 again, will remaining reagent discharges in the drain pipe 9 to in the waste liquid cup 3, the number of times of reagent rinse is 1 to 3 times. The rinsing step is used for when the required height of washing and data require the degree of accuracy to be high, the rinsing step only rinses the drain pipe, has both practiced thrift reagent and has prevented two kinds or multiple reagent cross contamination again.
In the step 2), the injection pump 5 pumps pure water to clean the liquid inlet pipe 8, the liquid outlet pipe 9 and the injection needle tube 15, so that cross infection among reagents is effectively avoided; residual pure water can be effectively discharged by air cleaning in the step 3), and liquid adding errors are avoided; in the first method in step 4), the liquid outlet pipe 9 firstly extracts and discharges the reagent, the liquid inlet pipe 8 then extracts air, and the residual reagent in the liquid outlet pipe 9 is discharged by using the discharged air; in the second method, the liquid outlet pipe 9 extracts the required reagent after extracting the air, and the liquid outlet pipe 9 firstly adds the required reagent and then exhausts the air during liquid adding, so that the reagent is completely exhausted, and a small amount of reagent is prevented from remaining on the inner wall of the liquid outlet pipe 9.
Claims (9)
1. A liquid adding method applied to a syringe pump is characterized in that: the method comprises the following steps:
step one, preparation
The vessel used in the method comprises a mother liquor bottle (1), a sample bottle (2), a waste liquor cup (3), a pure water reagent bottle (4) and an injection pump (5), wherein a Y-shaped valve (6) is arranged on the injection pump (5), and a Y-shaped valve (24) is respectively connected with a liquid inlet pipe (8), a liquid outlet pipe (9) and an injection needle tube (15);
step two, pure water cleaning
A liquid inlet pipe (8) is placed in a pure water reagent bottle (4), a liquid outlet pipe (9) is placed above a waste liquid cup (3), an injection pump (5) extracts pure water from the pure water reagent bottle (4) through the liquid inlet pipe (8), and the liquid inlet pipe (8), the liquid outlet pipe (9) and an injection needle tube (15) are cleaned sequentially by switching the liquid outlet direction of a Y-shaped valve (24);
step three, air cleaning
The liquid inlet pipe (8) is placed in the air or an empty reagent bottle, the injection pump (5) pumps the air through the liquid inlet pipe (8), and residual pure water in the liquid inlet pipe (8), the liquid outlet pipe (9) and the injection needle tube (15) is discharged completely by sequentially switching the air outlet direction of the Y-shaped valve (24);
step four, adding a reagent
Inserting a liquid outlet pipe (9) into a mother liquor bottle (1), pumping required amount of reagent by using an injection pump (5), inserting the liquid outlet pipe (9) into a sample bottle (2), injecting the reagent in the liquid outlet pipe (9) into the sample bottle (2), then pumping air by using the injection pump (5) through a liquid inlet pipe (8) and injecting the air in the liquid inlet pipe (8) into the sample bottle (2) through the liquid outlet pipe (9) so as to discharge the residual reagent in the liquid outlet pipe (9);
step five, adding other reagents
Repeating the operation of the second step and the third step, wiping the outer wall of the liquid outlet pipe (9), and repeating the operation of the fourth step to finish adding other reagents;
step six, finishing and cleaning
And repeating the operation of the second step and the third step, and then placing the liquid outlet pipe (9) above the waste liquid cup (3).
2. The priming method applied to the injection pump according to claim 1, characterized in that: the operation of the fourth step is as follows: after air is pumped by the injection pump (5) through the liquid outlet pipe (9), required amount of reagent is pumped from the mother liquid bottle (1), and then the air and the reagent in the liquid outlet pipe (9) are injected into the sample bottle (2).
3. The priming method applied to the syringe pump according to claim 1 or 2, characterized in that: and adding a reagent for rinsing between the third step and the fourth step, wherein the injection pump (5) pumps the reagent from the mother liquor bottle (1) through the liquid outlet pipe (9), then the reagent in the liquid outlet pipe (9) is discharged into the waste liquid cup (3), and the third step is repeated, so that the residual reagent in the liquid outlet pipe (9) is discharged into the waste liquid cup (3).
4. The priming method applied to the syringe pump according to claim 1 or 2, characterized in that: the injection needle tube (15) on the injection pump (5) is replaced to adapt to the requirements of different volumes, and the volume ranges from 100 microliters to 25 milliliters.
5. The priming method applied to the injection pump according to claim 1, characterized in that: the amount of air drawn in said fourth step cannot be less than 600 microlitres.
6. The priming method applied to the injection pump according to claim 2, characterized in that: the amount of extracted air cannot be less than 200 microliters.
7. The priming method applied to the syringe pump according to claim 1 or 2, characterized in that: the number of times of the pure water washing is 1 to 3 times.
8. The priming method applied to the syringe pump according to claim 1 or 2, characterized in that: the number of air purges is 2 to 3.
9. The priming method applied to the injection pump according to claim 3, characterized in that: the number of rinses of the reagent is from 1 to 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910821122.XA CN110658035A (en) | 2019-08-30 | 2019-08-30 | Liquid adding method applied to injection pump |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910821122.XA CN110658035A (en) | 2019-08-30 | 2019-08-30 | Liquid adding method applied to injection pump |
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| Publication Number | Publication Date |
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| CN110658035A true CN110658035A (en) | 2020-01-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910821122.XA Pending CN110658035A (en) | 2019-08-30 | 2019-08-30 | Liquid adding method applied to injection pump |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114455528A (en) * | 2021-12-30 | 2022-05-10 | 靖州玉华泉饮料有限公司 | Filling process of fruity mineral water beverage |
Citations (5)
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| US20110120213A1 (en) * | 2008-08-01 | 2011-05-26 | Shiseido Company, Ltd. | Sample injector, sample injecting method, and liquid chromatograph |
| CN203133096U (en) * | 2013-03-21 | 2013-08-14 | 浙江大学 | Full-automatic liquid sample collecting and feeding system without mixing and dead volume |
| CN106199032A (en) * | 2016-06-30 | 2016-12-07 | 南京润泽流体控制设备有限公司 | The sampling device of no cross contamination and control method thereof |
| CN106501539A (en) * | 2016-12-07 | 2017-03-15 | 苏州阿洛斯环境发生器有限公司 | A kind of sampling system and sample injection method for reducing dead volume liquid |
| CN109115579A (en) * | 2018-10-16 | 2019-01-01 | 湖北工业大学 | A kind of high throughput sample pre-treatments platform and liquid feeding method |
-
2019
- 2019-08-30 CN CN201910821122.XA patent/CN110658035A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110120213A1 (en) * | 2008-08-01 | 2011-05-26 | Shiseido Company, Ltd. | Sample injector, sample injecting method, and liquid chromatograph |
| CN203133096U (en) * | 2013-03-21 | 2013-08-14 | 浙江大学 | Full-automatic liquid sample collecting and feeding system without mixing and dead volume |
| CN106199032A (en) * | 2016-06-30 | 2016-12-07 | 南京润泽流体控制设备有限公司 | The sampling device of no cross contamination and control method thereof |
| CN106501539A (en) * | 2016-12-07 | 2017-03-15 | 苏州阿洛斯环境发生器有限公司 | A kind of sampling system and sample injection method for reducing dead volume liquid |
| CN109115579A (en) * | 2018-10-16 | 2019-01-01 | 湖北工业大学 | A kind of high throughput sample pre-treatments platform and liquid feeding method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114455528A (en) * | 2021-12-30 | 2022-05-10 | 靖州玉华泉饮料有限公司 | Filling process of fruity mineral water beverage |
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Application publication date: 20200107 |
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