CN203133096U - Full-automatic liquid sample collecting and feeding system without mixing and dead volume - Google Patents
Full-automatic liquid sample collecting and feeding system without mixing and dead volume Download PDFInfo
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- CN203133096U CN203133096U CN 201320131211 CN201320131211U CN203133096U CN 203133096 U CN203133096 U CN 203133096U CN 201320131211 CN201320131211 CN 201320131211 CN 201320131211 U CN201320131211 U CN 201320131211U CN 203133096 U CN203133096 U CN 203133096U
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Abstract
The utility model discloses a full-automatic liquid sample collecting and feeding system without mixing and dead volume. A microcontroller controls all electronic devices to automatically work, a special pipeline connection method is capable of realizing independent sample collection without interrupting a constant-flow injection analysis process, an injection pump is used for accurately collecting and feeding a sample, a sample collecting method including an air-isolation section is adopted and excessive 10 microliters of samples are collected when the sample is collected so as to realize the purpose that the sample is effectively protected, and the problem that the pressing on the volume of an air isolation section in a pipeline is reduced is solved by using a compensating means; and in addition, the full-automatic liquid collecting and feeding system is capable of realizing that the purpose that a pipeline and a sampling needle are automatically cleaned. According to the full-automatic liquid collecting and feeding system, the sample is prevented from being diluted by and mixed with a buffer solution or other liquids in the pipeline in a sample collecting and feeding process, the required sample can be accurately injected, no sample is remained in a pipeline system, and accurate and reliable sample collecting and feeding are provided for micro flow injection analysis.
Description
Technical field
The utility model relates to analysis to measure control technology field, relates in particular to a kind of Full Automatic Liquid sampler body sampling system that mixes no dead volume that do not have.
Background technology
The micro-amounts of liquids analytical technology is applied to multiple field, as environmental sample detection, Food Safety Analysis, clinical diagnosis, drug screening, biochemical research etc.Multiple fluid sample analysis instrument such as chromatographic analyzer of liquid phase, surface plasma resonance analyser etc. have high sensitivity for analysis, be fit to Accurate Analysis and detection to fluid sample, thereby also very high to the requirement of its liquid sample sampling system, not so can have a strong impact on analysis result.These analytical instrument, especially surface plasma resonance analyser, its analytic process and method are micro-Flow Injection Analysis, and be few to the sample consumption of required analysis, is about tens to one hectolambdas, thereby very high to the requirement of sampling sampling system.
Traditional fluid analysis sampling system generally is that constant flow pump and hand sampling valve are formed, and sample size is subjected to quantitative ring restriction and fixing immutable, and sample loads and injects and needs manual injection operation, brings the manual operation error, and labor intensive and time.Thereby produced automatic sampling sampling system, can control automatic collected specimens and sample injection by operation system of software, though automaticity has improved, and reduced the manual operation error, but systematic error still exists, particularly in the microanalysis process, systematic error is very big has influenced correctness and the stability of analysis result.
The factor that causes systematic error mainly is because the pipeline volume of sampling sampling system is generally the milliliter level, sample is cushioned liquid mixed diluting in various degree when entering before the detecting device in the sampling sampling system operation, perhaps because the pipe system complexity of sampling sampling system, there is dead volume, sample by in various degree remain in the sampling sampling system in, influence sample introduction analysis next time simultaneously.Therefore, present liquid sample sampling system, especially micro liquid sampling sampling system exist the more serious mixed dilution of sample and dead volume situation in the course of the work, cause for the analyzing and testing result and have a strong impact on.
The utility model content
The utility model provides a kind of Full Automatic Liquid sampler body sampling system that mixes no dead volume that do not have, especially be fit to micro liquid sampling and sample introduction automatically, the mixed dilution of sample and residual degree have greatly been reduced, for highly sensitive analyzing and testing device provides accurate and stable sampling and sample introduction.
For achieving the above object, the utility model is taked following technical scheme:
A kind of Full Automatic Liquid sampler body sampling system that does not have the no dead volume of mixing, comprise for the sampling mechanism of gathering testing sample, the damping fluid bottle that is used for the splendid attire damping fluid and first syringe pump and second syringe pump that is communicated with described damping fluid bottle respectively, also be provided with two six-way valves, these two six-way valves have first interface, second interface, the 3rd interface, the 4th interface, the 5th interface, the 6th interface, and two working positions of these two six-way valves are corresponding first connected state and second connected state respectively;
In described first connected state:
Sampling mechanism is communicated with successively with first interface, second interface, the 5th interface, the 6th interface, first syringe pump, damping fluid bottle, and second syringe pump is communicated with successively with the 4th interface, the 3rd interface, fluid sample analysis instrument;
In described second connected state:
Sampling mechanism is communicated with successively with first interface, the 6th interface, first syringe pump, damping fluid bottle, and second syringe pump is communicated with successively with the 4th interface, the 5th interface, second interface, the 3rd interface, fluid sample analysis instrument.
The utility model passes through specific pipeline connecting mode, and passes through the switching of two kinds of working positions of two six-way valves, realizes that sample mixes with the nothing of buffer solution, and can inject required sample size accurately.
As preferably, described sampling mechanism comprises:
Specimen holder;
Be used for driving the rotary drive mechanism of described specimen holder rotation;
Be erected at described specimen holder top and sampling needle that can lifting.
But the different testing sample of splendid attire in the specimen holder, when not sampling, sampling needle is away from specimen holder, when needs are sampled, only needs a control sampling needle drop to the sample place, rises after extracting required sample again; When needs are gathered the sample of diverse location, then utilize rotary drive mechanism to rotate specimen holder to desired location, the repeated sampling process gets final product.
As preferably, have on the described specimen holder and wash the pin mouth.After once sampling finishes, the pipeline that sample is flowed through and sampling needle need to clean: specimen holder is rotated to washing the pin mouth, the sampling needle insertion is washed in the pin mouth, in the container of first syringe pump with the damping fluid suction in the damping fluid bottle self, by pipeline fast injection damping fluid repeatedly, reach the effect of pipe blow-through and sampling needle then.
The lifting of described sampling needle needs an elevating mechanism to control, and as preferably, described sampling mechanism also is provided with for the lift drive mechanism that drives the sampling needle lifting, and this lift drive mechanism comprises:
The vertical screw mandrel of arranging;
Be used for driving first stepper motor that described screw mandrel rotates;
The crane that cooperates with described wire rod thread, and described sampling needle is fixed on this crane.
Wherein, the rotation of first stepper motor can drive screw mandrel, and then the lifting of control crane drive sampling needle, samples.
Further preferred, also be provided with the control system for control lift drive mechanism, rotary drive mechanism, first syringe pump, second syringe pump, two six-way valves in the utility model device.Above each electron device co-ordination of this control system control, as control first syringe pump, second syringe pump work, control two six-way valves switching, carry out position probing according to Hall element, and then stepper motor driver controlled etc., and then realize microsampling and the sample introduction work of fluid sample.
Be the accurately height of control sampling needle, as preferably, described sampling mechanism also comprises first Hall element that detects the sampling needle height, described control system according to the signal of this first Hall element to control first stepper motor.
Further preferred, described first Hall element is two, the extreme position that the respectively corresponding sampling needle in its locus rises and descends, the extreme position of sampling needle when namely not sampling and sampling.
In like manner, be accurately to gather the sample of diverse location, as preferably, described rotary drive mechanism is for by second stepper motor of second web member with the coaxial setting of specimen holder.
Further preferred, described sampling mechanism also comprises second Hall element that the test sample chord position is put, described control system according to the signal of this second Hall element to control second stepper motor.
Control system receives the signal of second Hall element, requires control second stepper motor to rotate according to sampling, and then drives the specimen holder rotation, makes sampling needle aim at the sample of ad-hoc location on the specimen holder, samples.
The principle of work of the utility model Full Automatic Liquid sampler body sampling system is as follows:
During holding state, in the container of second syringe pump with the damping fluid suction in the damping fluid bottle self, enter the 4th interface of two six-way valves then by pipeline, and flow out through the 3rd interface, to sample analyser constant current injection damping fluid; Gather the sample of required analysis as need, by second stepper motor specimen holder is rotated, make the sample of required collection rotate below to sampling needle, by first stepper motor sampling needle is moved up and down, sampling needle is inserted in the sample, first syringe pump is gathered sample to pipeline by first interface, second interface, the 5th interface, the 6th interface of two six-way valves successively, by switching two six-way valve positions, the sample in the pipeline is delivered to by second syringe pump and is carried out analyzing and testing in the sample analyser.
When the holding state of sample analyser, generally monitor background signal in constant flow rate running buffer mode, all be full of damping fluid in the pipe system this moment, can contact with damping fluid when sample is gathered to the pipe system and walk simultaneously, and sample can be cushioned the liquid diluted mixture.Therefore, before collected specimens, at first sampling needle is in and gathers air 5 microlitres in the air, insert collected specimens 5 microlitres in the sample then, be in again and gather air 5 microlitres in the air, carry out real sample collection again after finishing above three steps, the sample size of its collection adds 10 microlitres again on the sample size basis of required analysis, after finishing the sample collection, gather the work of each 5 microlitre of air-sample-air again, protecting the sample of middle required analysis to avoid contacting damping fluid by this mode moves in pipeline, in sample enters pipeline between second interface and the 5th interface, and keep in the pipeline of the unnecessary sample of 5 microlitres between sampling needle and first interface; Can begin sample introduction work by second syringe pump by switching two six-way valve positions, second syringe pump guarantees the accurate of injected sample amount according to accurately controlling the step number of advancing.
Because during sample injection, have the air insulated section to move after sample in the pipeline advances thereupon, air is because being subjected to the pipeline inner pressure volume compression, during this period of time, the sample travelling speed is lower than the setting travelling speed, till air section is compressed to degree of stability, therefore makes second syringe pump move minimum step number at a relatively high speed before real injection, make air section fast compressed and reach steady state (SS), begin to carry out real sample injection again.
The nothing that the utility model provides is mixed the Full Automatic Liquid sampler body sampling system of no dead volume; automatically controlling each electron device by control system works automatically; and specific pipeline is selected for use and connected mode; and employing contains fluid sample collection and the input mode of air insulated protection; avoid sample in collection and sample introduction process, in pipeline, to be cushioned liquid or the mixing of other liquid dilutings; and can inject required sample size accurately; n.s. residues in the pipe system, for micro-Flow Injection Analysis provides accurate and reliable sampling and sample introduction.
Description of drawings
Fig. 1 is the structural representation of Full Automatic Liquid sampler body sampling system of the present utility model;
A partly is two internal communication synoptic diagram under a kind of location status of six-way valve among Fig. 2;
B partly is the internal communication synoptic diagram under the another kind of location status of two six-way valves among Fig. 2;
Fig. 3 is the microcomputer control synoptic diagram of Full Automatic Liquid sampler body sampling system of the present utility model;
Fig. 4 is the sample mode synoptic diagram that utilizes the air insulated of the utility model Full Automatic Liquid sampler body sampling system.
Embodiment
Fig. 1 shows the structural representation that the utility model does not have the Full Automatic Liquid sampler body sampling system that mixes no dead volume, and its physical construction comprises:
Sampling mechanism, damping fluid bottle 1, first syringe pump 2 that is communicated with the damping fluid bottle respectively and second syringe pump 3, also be provided with two six-way valves 4, these two six-way valves have the first interface 4-1, the second interface 4-2, the 3rd interface 4-3, the 4th interface 4-4, the 5th interface 4-5, the 6th interface 4-6.
Wherein, sampling mechanism comprises:
Be erected at the sampling needle 9 of specimen holder top;
Sample for ease of sampling needle 9, also be provided with the lift drive mechanism that drives sampling needle 9 liftings, this lift drive mechanism comprises:
The vertical screw mandrel of arranging 10;
Drive first stepper motor 11 that described screw mandrel rotates; Link to each other by first web member 12 between screw mandrel 10 and first stepper motor 11;
With the crane 13 of screw mandrel 10 threaded engagement, sampling needle 9 is fixed on this crane.
This sampling mechanism also comprises and is individually fixed in screw mandrel by two 14, two first Hall elements 14 of first Hall element that detect the sampling needle height 10 about two positions, also comprises second Hall element 15 that the test sample chord position is put, and is fixed in below the specimen holder.
Also be provided with the control system for control first stepper motor 11, second stepper motor 8, first syringe pump 2, second syringe pump 3, two six-way valves 4 in the utility model device.This control system according to the signal of first Hall element controlling first stepper motor, according to the signal of second Hall element to control second stepper motor.
All pipelines all adopt the Teflon pipe in the said apparatus, and specification is: 0.03 inch of first pipeline, 16 internal diameter, and 1/16 inch of external diameter, length gets final product at the bottom of only needing enough to insert the damping fluid bottle; 0.03 inch of second pipeline, 17 internal diameter, 1/16 inch of external diameter, length is tried one's best to lack under the situation that enough connects two parts and is got final product; 0.03 inch of the 3rd pipeline 18 internal diameter, 1/16 inch of external diameter, length is 280mm; 0.02 inch of the 4th pipeline 19 internal diameter, 1/16 inch of external diameter, length is tried one's best to lack under the situation that enough connects two parts and is got final product; 0.006 inch of the 5th pipeline 20 internal diameter, 1/16 inch of external diameter, length is tried one's best to lack under the situation that enough connects two parts and is got final product.
For ease of washing the collection of waste liquid behind the pin, to wash pin mouth place in the specimen holder aligned beneath and be provided with Drainage pipe 22, this Drainage pipe can adopt internal diameter 3mm, the plastics of external diameter 6mm or silicone tube, length only need insert waste liquid bottle 23 and get final product.
As shown in Figure 2, communicate in twos between six interfaces of two six-way valves, have two kinds of location statuss, can be by the control system location status that automaticallyes switch.
As shown in Figure 3, control system is microcomputerized controller, is master chip with C8051F020, comprises serial expanded circuit, IO mouth circuit and PCA circuit, is used for control syringe pump, two six-way valves, stepper motor work automatically and detects the Hall element position.
When the holding state that biosensor analysis detects, generally monitor background signal in constant flow rate running buffer mode, all be full of damping fluid in the pipe system this moment, this moment i.e. first working position of two six-way valves: sampling mechanism is communicated with successively with the first interface 4-1, the second interface 4-2, the 5th interface 4-5, the 6th interface 4-6, first syringe pump 2, damping fluid bottle 1, and second syringe pump 3 is communicated with successively with the 4th interface 4-4, the 3rd interface 4-3, sample analyser 21.
Can contact with damping fluid when sample is gathered to the pipe system and walking simultaneously, sample can be cushioned the liquid diluted mixture.Therefore, before collected specimens, at first sampling needle is in and gathers air 5 microlitres in the air, insert collected specimens 5 microlitres in the sample then, be in again and gather air 5 microlitres in the air, carry out real sample collection again after finishing above three steps, the sample size of its collection adds 10 microlitres again on the sample size basis of required analysis, after finishing the sample collection, gather the work of each 5 microlitre of air-sample-air again, as shown in Figure 4, protect the sample of middle required analysis to avoid contacting damping fluid by this mode and in pipeline, move, in sample enters the 3rd pipeline, and keep the unnecessary sample of 5 microlitres in the 4th pipeline; By switching a working position, two six-way valve positions to the second: namely sampling mechanism is communicated with successively with the first interface 4-1, the 6th interface 4-6, first syringe pump 2, damping fluid bottle 1, and second syringe pump 3 is communicated with successively with the 4th interface 4-4, the 5th interface 4-5, the second interface 4-2, the 3rd interface 4-3, sample analyser 21.At this moment, can begin the sample introduction work by second syringe pump 3, second syringe pump 3 guarantees the accurate of injected sample amount according to accurately controlling the step number of advancing.
Because during sample injection, have the air insulated section to move after sample in the pipeline advances thereupon, air is because being subjected to the pipeline inner pressure volume compression, during this period of time, the sample travelling speed is lower than the setting travelling speed, till air section is compressed to degree of stability, therefore makes second syringe pump 3 move minimum step number at a relatively high speed before real injection, make air section fast compressed and reach steady state (SS), begin to carry out real sample injection again.
Finishing sample injection back the 3rd pipeline 18, the 4th pipeline 19 and sampling needle 9 needs to clean, with specimen holder 5 rotations, sampling needle 9 insertions are washed in the pin mouth 6, first syringe pump 2 is by in the container of first pipeline 16 with the damping fluid suction in the damping fluid bottle self, pass through second pipeline 17 and two six-way valve 4 fast injection damping fluids repeatedly then, reach the purpose of cleaning the 3rd pipeline 18 and the 4th pipeline 19 and sampling needle 9.
Claims (9)
1. one kind is not had the Full Automatic Liquid sampler body sampling system that mixes no dead volume, comprise for the sampling mechanism of gathering testing sample, the damping fluid bottle that is used for the splendid attire damping fluid and first syringe pump and second syringe pump that is communicated with described damping fluid bottle respectively, it is characterized in that, also be provided with two six-way valves, these two six-way valves have first interface, second interface, the 3rd interface, the 4th interface, the 5th interface, the 6th interface, and two working positions of these two six-way valves are corresponding first connected state and second connected state respectively;
In described first connected state:
Sampling mechanism is communicated with successively with first interface, second interface, the 5th interface, the 6th interface, first syringe pump, damping fluid bottle, and second syringe pump is communicated with successively with the 4th interface, the 3rd interface, sample analyser;
In described second connected state:
Sampling mechanism is communicated with successively with first interface, the 6th interface, first syringe pump, damping fluid bottle, and second syringe pump is communicated with successively with the 4th interface, the 5th interface, second interface, the 3rd interface, sample analyser.
2. nothing according to claim 1 is mixed the Full Automatic Liquid sampler body sampling system of no dead volume, it is characterized in that described sampling mechanism comprises:
Specimen holder;
Be used for driving the rotary drive mechanism of described specimen holder rotation;
Be erected at described specimen holder top and sampling needle that can lifting.
3. nothing according to claim 2 is mixed the Full Automatic Liquid sampler body sampling system of no dead volume, it is characterized in that, has on the described specimen holder and washes the pin mouth.
4. nothing according to claim 2 is mixed the Full Automatic Liquid sampler body sampling system of no dead volume, it is characterized in that, described sampling mechanism also is provided with for the lift drive mechanism that drives the sampling needle lifting, and this lift drive mechanism comprises:
The vertical screw mandrel of arranging;
Be used for driving first stepper motor that described screw mandrel rotates;
The crane that cooperates with described wire rod thread, and described sampling needle is fixed on this crane.
5. nothing according to claim 4 is mixed the Full Automatic Liquid sampler body sampling system of no dead volume, it is characterized in that, also is provided with the control system for control lift drive mechanism, rotary drive mechanism, first syringe pump, second syringe pump, two six-way valves.
6. nothing according to claim 5 is mixed the Full Automatic Liquid sampler body sampling system of no dead volume, it is characterized in that, described sampling mechanism also comprises first Hall element that detects the sampling needle height, described control system according to the signal of this first Hall element to control first stepper motor.
7. nothing according to claim 6 is mixed the Full Automatic Liquid sampler body sampling system of no dead volume, it is characterized in that described first Hall element is two, the extreme position that the respectively corresponding sampling needle in its locus rises and descends.
8. nothing according to claim 7 is mixed the Full Automatic Liquid sampler body sampling system of no dead volume, it is characterized in that, described rotary drive mechanism is by second stepper motor of second web member with the coaxial setting of specimen holder.
9. nothing according to claim 8 is mixed the Full Automatic Liquid sampler body sampling system of no dead volume, it is characterized in that, described sampling mechanism also comprises second Hall element that the test sample chord position is put, described control system according to the signal of this second Hall element to control second stepper motor.
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| CN105823727A (en) * | 2016-05-05 | 2016-08-03 | 北京纳迅科技股份有限公司 | Microfluid control detecting device and system |
| CN108067156A (en) * | 2018-01-04 | 2018-05-25 | 四川汉辉盛世医疗科技有限公司 | A kind of intelligence liquid mixing system and method |
| CN108614033A (en) * | 2017-06-14 | 2018-10-02 | 中国科学院过程工程研究所 | A kind of moveable mobile phase control system and its processing method and purposes with double gradient regulatory functions |
| CN109827804A (en) * | 2019-01-16 | 2019-05-31 | 深圳供电局有限公司 | Injection device and collection system |
| CN110462398A (en) * | 2017-04-04 | 2019-11-15 | 奥加诺株式会社 | Method and analytical equipment for quantitative urea |
| CN110531100A (en) * | 2019-08-30 | 2019-12-03 | 中国航发北京航空材料研究院 | A kind of liquid feeding method |
| CN110658035A (en) * | 2019-08-30 | 2020-01-07 | 中国航发北京航空材料研究院 | Liquid adding method applied to injection pump |
| CN111830270A (en) * | 2020-07-09 | 2020-10-27 | 迪瑞医疗科技股份有限公司 | Precise sample adding system, in-vitro diagnosis equipment and precise sample adding method |
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| CN112379032A (en) * | 2020-10-26 | 2021-02-19 | 安徽皖仪科技股份有限公司 | Automatic sample injector, liquid chromatograph and sample injection method of liquid chromatograph |
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| CN105823727A (en) * | 2016-05-05 | 2016-08-03 | 北京纳迅科技股份有限公司 | Microfluid control detecting device and system |
| CN105823727B (en) * | 2016-05-05 | 2018-10-23 | 北京纳迅科技股份有限公司 | A kind of micro-fluidic detection device and system |
| CN110462398A (en) * | 2017-04-04 | 2019-11-15 | 奥加诺株式会社 | Method and analytical equipment for quantitative urea |
| CN108614033A (en) * | 2017-06-14 | 2018-10-02 | 中国科学院过程工程研究所 | A kind of moveable mobile phase control system and its processing method and purposes with double gradient regulatory functions |
| CN108067156A (en) * | 2018-01-04 | 2018-05-25 | 四川汉辉盛世医疗科技有限公司 | A kind of intelligence liquid mixing system and method |
| CN109827804A (en) * | 2019-01-16 | 2019-05-31 | 深圳供电局有限公司 | Injection device and collection system |
| CN110531100A (en) * | 2019-08-30 | 2019-12-03 | 中国航发北京航空材料研究院 | A kind of liquid feeding method |
| CN110658035A (en) * | 2019-08-30 | 2020-01-07 | 中国航发北京航空材料研究院 | Liquid adding method applied to injection pump |
| CN112748253B (en) * | 2019-10-31 | 2024-06-07 | 深圳市帝迈生物技术有限公司 | Sample adding device, sample adding method thereof and computer storage medium |
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| CN111856055A (en) * | 2020-07-27 | 2020-10-30 | 长沙开元仪器有限公司 | Feeding mechanism |
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| CN114441236A (en) * | 2020-11-03 | 2022-05-06 | 深圳市帝迈生物技术有限公司 | Sampling device and sample analyzer |
| CN112362794A (en) * | 2020-11-10 | 2021-02-12 | 山东悟空仪器有限公司 | Sample feeder, full-loop sample feeding method, liquid analysis method and liquid chromatography system |
| CN114088648A (en) * | 2021-12-07 | 2022-02-25 | 广东盈峰科技有限公司 | Gas-liquid dual isolation method for multi-ported valve micro-reagent sampling |
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