CN105334332A - Sample analyzer and sample collection and distribution method thereof - Google Patents
Sample analyzer and sample collection and distribution method thereof Download PDFInfo
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- CN105334332A CN105334332A CN201410311060.5A CN201410311060A CN105334332A CN 105334332 A CN105334332 A CN 105334332A CN 201410311060 A CN201410311060 A CN 201410311060A CN 105334332 A CN105334332 A CN 105334332A
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Abstract
A sample analyzer and a sample collection and distribution method thereof are provided. A sample collection and distribution module conducts a first collection on a sample to be tested; and then the collected sample is assigned to a plurality of measurement modules according to a predetermined sequence. Compared to a way of multiple sample collection and multiple sample distribution, the method provided by the invention simplifies the steps of sample collection and distribution, improves the test efficiency of liquid samples, and avoids the risk of sample contamination caused by a plurality of sample collections.
Description
Technical field
The application relates to liquid sample detection field, is specifically related to a kind of sample analyser and sample collection thereof and distribution method.
Background technology
Clinically, often need to utilize a blood sample, detect multiple hematological parameter, such as blood routine parameter and CRP (C-ReactiveProtein, C reactive protein) parameter etc.
On the one hand, because blood count, classification are use dissimilar sample to carry out on different blood analysis instrument from the detection of CRP.Therefore, usually use whole blood sample in the enterprising promoting circulation of blood cell count of blood cell analyzer and classification, use serum to measure CRP on biochemical analysis or Special Protein Analyzer.And blood routine parameter and CRP usually conbined usage clinically, medical worker needs to gather twice sample at patient, tests respectively on different machines.For patient, the misery that twice sample collection causes is comparatively large, and needs to detect on two machines, detects troublesome poeration.
On the other hand, in order to realize detecting routine blood test and CRP parameter rapidly, the measure portion of detecting instrument must can complete routine blood test count measurement and CRP measures.Because routine blood test is all not identical with the measuring method that CRP uses, reagent used, separately at least two passages are therefore needed to measure respectively.Now, each sample gathered is only for a channel measurement, and multiple channel sample then needs corresponding collection and distributes repeatedly.Need after each sample collection is assigned to clean inside and outside sampling needle, must limit to some extent the speed of whole measurement procedure like this, the object detected fast cannot be realized when continuous sample is measured.In addition, sampling needle repeatedly enters sample when gathering, and the risk of by sampling needle extraneous foreign matter being brought into sample is increased, and easily pollutes sample.Please refer to Fig. 1, is the schematic flow sheet of sample collection and distribution.Wherein, the sample of each passage needs to gather separately and distribute.In order to avoid sample cross contamination between passage, after gathering each time, being assigned, need to clean inside and outside sampling needle, the sample of the collection and lower the same passage of carrying out next sample again distributes, thus need to consume a large amount of time, the speed of blood testing is limited to some extent, and easily increases the contaminated risk of sample.
Summary of the invention
The application provides a kind of sample analyser and sample collection thereof and distribution method, solves blood testing inefficiency, the easy contaminated problem of sample.
According to the first aspect of the application, this application provides a kind of sample analyser, comprising:
Multiple measurement module, the liquid sample that each measurement module is used for distributing carries out by the measurement obtained for the purpose of different parameters;
Sample collection distribution module, for collecting sample, and distributes to each measurement module by the sample of collection;
Fluid path support module, for each measurement module and sample collection distribution module provide fluid path support;
Control module, it is coupled to sample collection distribution module, once gathering, then the sample of collection being distributed to each measurement module according to predefined procedure for controlling sample collection distribution module to sample to be detected.
According to the second aspect of the application, this application provides a kind of sample collection and distribution method of sample analyser, described sample analyser comprises:
Multiple measurement module, the liquid sample that each measurement module is used for distributing carries out by the measurement obtained for the purpose of different parameters;
Sample collection distribution module, for collecting sample, and distributes to each measurement module by the sample of collection;
Fluid path support module, for each measurement module and sample collection distribution module provide fluid path support;
Control module, it is coupled to sample collection distribution module;
Described method comprises: the sample collection distribution module of sample analyser once gathers sample to be detected, then the sample of collection is distributed to each measurement module according to predefined procedure.
A kind of sample analyser that the application provides and sample collection thereof and distribution method, sample collection distribution module once gathers sample to be detected, then the sample of collection is distributed to multiple measurement module according to predefined procedure.Compared to the mode that repeatedly sample collection, repeatedly sample distribute, simplify the step of sample collection and distribution, improve the efficiency that liquid sample detects, and avoid the risk that repeatedly sample collection pollutes sample.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of sample collection and distribution method in prior art;
Fig. 2 is the structural representation of blood detector in a kind of embodiment of the application;
Fig. 3 is the structural representation of sample collection distribution module in a kind of embodiment blood detector of the application;
Fig. 4 is the structural representation of sampling needle in a kind of embodiment of the application;
Fig. 5 is the structural representation of sampling needle in the another kind of embodiment of the application;
Fig. 6 is the schematic flow sheet of sample collection and distribution method in a kind of embodiment of the application;
Fig. 7 is the sample view of different phase down-sampling pin inside in a kind of embodiment sample of the application assigning process.
Embodiment
The embodiment of the present application provides a kind of sample analyser and sample collection thereof and distribution method, this sample analyser can be the instrument that blood detector, Biochemical Analyzer etc. need to carry out liquid acquisition and distribution, for the ease of the understanding to the application, be described for blood detector in the embodiment of the present application.
By reference to the accompanying drawings the application is described in further detail below by embodiment.
Embodiment one
Please refer to Fig. 2, present embodiments provide a kind of blood detector, comprise sample feeding module 10, measurement module 30, sample collection distribution module 20, fluid path support module and control module.
Sample feeding module 10 is for loading sample to be tested.
Measurement module 30 has multiple, and each measurement module 30 is for carrying out the liquid sample distributed by the measurement obtained for the purpose of different parameters.In a particular embodiment, as shown in Figure 2, measurement module 30 can comprise WBC category measurement module 301, CRP measurement module 302 and routine blood test count measurement module 303.Liquid sample can be whole blood sample.
The sample of collection for collecting sample, and is distributed to each measurement module 30 by sample collection distribution module 20.
Fluid path support module (accompanying drawing is not shown) provides fluid path support for each measurement module 30 and sample collection distribution module 20.
Control module (accompanying drawing is not shown) is coupled to sample collection distribution module 20, once gathering, then the sample of collection being distributed to each measurement module 30 according to predefined procedure for controlling sample collection distribution module 20 to sample to be detected.
Please refer to Fig. 3, in a particular embodiment, sample collection distribution module 20 comprises fixed support 201, X-direction guide rail 202, X-direction gear train 203, travel(l)ing rest 204, Y-direction guide rail 205, Y-direction gear train 206, sampling needle 207 and swab 208.
Fixed support 201 is fixed on the main body rack of blood detector, travel(l)ing rest 204 is formed by X-direction guide rail 202, X-direction running part 203 and fixed support 201 and is slidably connected, and sliding support 204 and parts mounted thereto can be moved in X direction under the driving of X-direction gear train 203.Sampling needle 207 to be formed with travel(l)ing rest 204 by Y-direction guide rail 205, Y-direction running part 206 and is slidably connected, and sampling needle 207 can be moved as Y-direction by relative motion support 204 under the driving of gear train 206 in the Y direction.Swab 208 is for cleaning sampling needle 207, and when sampling needle 207 carries out Y-direction motion, swab 208 provides liquid rinse sampling needle by fluid path support module, and is taken away by the liquid after cleaning simultaneously.
Sample collection distribution module 20 is when carrying out sample collection, by X-direction gear train 203, travel(l)ing rest 204 is moved to the position at sample feeding module 10 sample place, then by Y-direction gear train 206, sampling needle 207 is moved down into sample feeding module 10 inside.Now, it is inner at sampling needle 207 that the power that sampling needle 207 can be provided by fluid path support module draws quantitative sample storage, completes sample collection action.
Sample collection distribution module 20 is when carrying out sample and distributing, by X-direction gear train 203, travel(l)ing rest 204 is moved to above corresponding measurement module 30, by Y-direction gear train 206, sampling needle 207 is moved down into measure portion 30 inside again, power is provided afterwards by fluid path support module, the sample being stored in sampling needle 207 inside is quantitatively released, completes sample and distribute action.
When sample collection distribution module 20 needs to clean sampling needle 207, by Y-direction gear train 206, sampling needle 207 is moved to swab 208 inside, swab 208 pairs of sampling needles 207 clean.Usually, swab 208 can clean the outer wall of sampling needle 207, if need to clean sampling needle 207 inside, needs to inject liquid by fluid path support module to sampling needle 207 inside, and is collected by swab 208, to realize the cleaning to sampling needle 207 inside.
Preferably, control module also for controlling sample collection distribution module 20 when distributing sample to measurement module 30, under the liquid level first pin hole of sampling needle 207 being stretched into measurement module 30 end liquid, then to injecting sample in measurement module.End liquid in measurement module 30 can be hemolytic agent.
After sample collection distribution module 20 carries out a sample collection, needs carry out rationed continuously in multiple measurement module.For general detecting instrument, the amount of the sample needed for each measurement module is less, large about a few microlitre to tens microliters, when carrying out sample and distributing, the pin hole controlling sampling needle 207 is positioned under the liquid level of liquid at the bottom of measurement module, can ensure that sample can not be stained with sampling needle 207 outer wall in a large number, thus affect rationed.
Because sampling needle 207 needle point enters the problem bringing cross pollution in liquid at the bottom of measurement module, such as end liquid may have influence on the purity of sampling needle 207 inside residue sample.
For solving the problem, further, the sample of preset dose in sampling needle, also for controlling sample collection distribution module 20 after distributing sample to previous measurement module, before distributing sample, is abandoned to next measurement module by control module.After being abandoned by the sample being diluted by end liquid or pollute, this section of sample can be avoided to affect the measurement result of next measurement module, ensure that two adjacent measurement modules sample used does not exist cross pollution.
In addition, please refer to Fig. 4, in a particular embodiment, the diameter D2 of the pin hole 2072 of sampling needle 207 is less than the internal diameter D1 of sampling needle needle tubing 2071.Because end liquid is mainly transmitted by sampling needle pin hole 2072 cross pollution that the sample in sampling needle 207 produces, the diameter D2 therefore reducing sampling needle pin hole 2072 effectively can reduce the degree of cross pollution.
At present, in most of medical diagnostic equipment, in order to avoid pathology sample Infection Action person, the mode that sample adopts sealing to preserve usually, such as, sample is stored in the test tube of band rubber cap after acquisition target gathers.Therefore, the mode of sample collection distribution module 20 collecting sample of blood analyser can be open mode and closed mode two kinds.Open mode refers to that operator is loaded into sample feeding module 10 after being opened by test tube rubber cap, and sampling needle 207 enters in vitro draws sample.Closed mode refers to that test tube rubber cap is not opened, and is directly loaded into sample feeding module 10, and sampling needle 207 enters test tube interior and draws sample after puncturing test tube rubber cap.
Please refer to Fig. 5, for adapting to above-mentioned closed mode, in another embodiment, the needle point 2073 of sampling needle 207 is cone, and the pin hole 2072 of sampling needle 207 is positioned at the sidewall bottom needle tubing 2071.The needle point 2073 of sampling needle 207 is designed to cone, needle point area can be reduced when sampling needle 207 punctures test tube rubber cap, improve pressure, allow sampling needle 207 can wear out test tube rubber cap smoothly, or 2073 also can be that pyramid (triangular pyramid, rectangular pyramid etc.), scarf, roundlet are first-class, as long as the profile that can be conducive to puncturing can.Meanwhile, the pin hole 2072 of sampling needle 207 is positioned at the sidewall bottom needle tubing 2071, and can avoid in piercing process, the rubber scraps that rubber cap comes off blocks sampling needle pin hole 2072.For without the need to puncture, or not with the sample in the test tube of rubber cap, sampling needle 207 can not have needle point 2073.
For closed mode, because test tube interior pressure and ambient pressure there are differences, enter at sampling needle 207, when leaving test tube, the pressure at pin hole 2072 place sudden transformation can occur.And sampling needle 207 and the pipeline that is connected with sampling needle 207 have certain elasticity, when pin hole 2072 place Pressure Step changes, there is certain displacement in the sample stored in sampling needle 207, thus cause carrying out producing error when sample is rationed.
For solving the problem, in a particular embodiment, the sample of preset dose in sampling needle 207, also for controlling sample collection distribution module 20 before carrying out first sample distribution, is abandoned by control module.
Further, control module is also for controlling amount that sample collection distribution module 20 once gathers sample to be detected for the amount sum of the sample that the amount of each measurement module sample and sample collection distribution module are abandoned.To guarantee as each measurement module carries out rationed.
In a particular embodiment, control module also for controlling sample collection distribution module 20 after the measurement module 30 used to all needs distributes sample, is cleaned sampling needle 207.Now, in sampling needle 207, also can remaining sample, when cleaning sampling needle 207, this remaining sample having been abandoned.
It should be noted that, in the blood detector that the present embodiment provides, owing to only once gathering sample, amount sufficiently all measurement module uses of therefore sample collection, also need the loss comprising other, such as sample adhesion is at sampling needle 207 inwall simultaneously.Due to sampling needle 207 latter end sebific duct coupling part there is step, corner characteristics is waited for death in gap, sample can by liquid diluting original in pipeline through these features, causes quantitatively inaccurate, affects measurement result.Therefore, when being temporarily stored in sampling needle 207 inside after sample collection, sample liquid level can not exceed the top latter end of sampling needle 207 needle tubing.
In the blood detector that the present embodiment provides, control module once gathers sample to be detected for controlling sample collection distribution module 20, then the sample of collection is distributed to each measurement module 30 according to predefined procedure.Only carry out a sample collection, collection allocation flow can be had by reduced sample, improve the efficiency of blood testing, sampling needle 207 multiple-contact sample can also be avoided simultaneously, thus bring the problem of pollution.
Embodiment two
Based on the blood detector that above-described embodiment one provides, corresponding sample collection and the distribution method providing a kind of blood detector of the present embodiment, comprise: the sample collection distribution module of blood detector once gathers sample to be detected, then the sample of collection is distributed to each measurement module according to predefined procedure.
Preferably, sample collection distribution module distributes sample to measurement module, is specially: the pin hole of sampling needle first stretches under the liquid level of liquid at the bottom of measurement module by sample collection distribution module, then to injecting sample in measurement module.End liquid in measurement module can be hemolytic agent.
After sample collection distribution module carries out a sample collection, needs carry out rationed continuously in multiple measurement module.For general detecting instrument, the amount of the sample needed for each measurement module is less, large about a few microlitre to tens microliters, when carrying out sample and distributing, the pin hole of sampling needle first stretches under the liquid level of liquid at the bottom of measurement module by sample collection distribution module, again to injecting sample in measurement module, can ensure that sample can not be stained with sampling needle outer wall in a large number, thus affect rationed.
But the pin hole of sampling needle first stretches under the liquid level of liquid at the bottom of measurement module by sample collection distribution module, then to injecting sample in measurement module, can bring the problem of cross pollution, such as end liquid may have influence on the purity of sampling needle inside residue sample.
For solving the problem, further, in sample collection distribution module to after previous measurement module distributes sample, before distributing sample to next measurement module, also comprise: the sample of preset dose in sampling needle is abandoned by sample collection distribution module.After being abandoned by the sample being diluted by end liquid or pollute, this section of sample can be avoided to affect the measurement result of next measurement module, ensure that two adjacent measurement modules sample used does not exist cross pollution.
At present, in most of medical diagnostic equipment, in order to avoid pathology sample Infection Action person, the mode that sample adopts sealing to preserve usually, such as, sample is stored in the test tube of band rubber cap after acquisition target gathers.Therefore, the mode of the sample collection distribution module collecting sample of blood analyser can be open mode and closed mode two kinds.Open mode refers to that operator is loaded into sample feeding module after being opened by test tube rubber cap, and sampling needle enters in vitro draws sample.Closed mode refers to that test tube rubber cap is not opened, and is directly loaded into sample feeding module, and sampling needle enters test tube interior and draws sample after puncturing test tube rubber cap.
For closed mode, because test tube interior pressure and ambient pressure there are differences, enter at sampling needle, when leaving test tube, the pressure at pin hole place sudden transformation can occur.And sampling needle and the pipeline that is connected with sampling needle have certain elasticity, when pin hole place Pressure Step changes, there is certain displacement in the sample stored in sampling needle, thus cause carrying out producing error when sample is rationed.
For solving the problem, in the present embodiment, before sample collection distribution module carries out first sample distribution, also comprise: the sample of preset dose in sampling needle is abandoned by sample collection distribution module.
Further, in the step that sample collection distribution module once gathers sample to be detected, amount that sample collection distribution module once gathers sample to be detected is the amount sum of the sample that the amount of sample required for each measurement module and sample collection distribution module are abandoned.To guarantee as each measurement module carries out rationed.
In a particular embodiment, after sample collection distribution module distributes sample to the measurement module that all needs use, also comprise: the sampling needle of sample collection distribution module is cleaned.Now, when cleaning sampling needle, in sampling needle, also can remain sample (latter end sample), while cleaning, this remaining sample having been abandoned.
It should be noted that, in the method that the present embodiment provides, owing to only once gathering sample, amount sufficiently all measurement module uses of therefore sample collection, also need the loss comprising other, such as sample adhesion is at sampling needle inwall simultaneously.Due to sampling needle latter end sebific duct coupling part there is step, corner characteristics is waited for death in gap, sample can by liquid diluting original in pipeline through these features, causes quantitatively inaccurate, affects measurement result.Therefore, in the step that sample collection distribution module gathers sample, sample liquid level can not exceed the top latter end of sampling needle needle tubing.
Below, composition graphs 6 and Fig. 7, be described further the method that the present embodiment provides, Fig. 6 and Fig. 7 distributes sample for needs to three measurement modules and be described.The schematic flow sheet of the method that Fig. 6 provides for the present embodiment, it specifically can comprise step below:
Step 601: the sample collection distribution module of blood detector once gathers sample to be detected, as shown in Figure 7a, the sample now in sampling needle comprises the amount of its sample once gathered: abandon sample 701, first measurement module for first section and distribute sample 702, first isolation and abandon sample 703, second measurement module and distribute that sample 705 is abandoned in sample 704, second isolation, the 3rd measurement module distributes sample 706 and latter end and abandons sample 707.Should be appreciated that sample 701-707 is in fact the same, using different modes to distinguish it in Fig. 7 is in order to the step that sample distributes is described more intuitively.
Step 602: before sample collection distribution module carries out first sample distribution, the sample of preset dose in sampling needle is abandoned by sample collection distribution module, and after abandoning first section of sample, the sample state in sampling needle as shown in Figure 7b.When sampling needle needs to abandon the sample of inside, sampling needle first rises and makes needle point arrive swab inside, power is provided by fluid path support module, the sample of sampling needle internal preset dosage is released outside pin and abandon, the sample be pushed out can be collected by swab and abandon, and sample has been abandoned rear swab and cleaned sampling needle needle point outer wall.
Step 603: sample collection distribution module distributes sample to measurement module 1, after distributing, the sample state in sampling needle as shown in Figure 7 c.
Step 604: the distance piece sample of preset dose is abandoned by sample collection distribution module, after abandoning, the sample state in sampling needle as shown in figure 7d.
Step 605: sample collection distribution module distributes sample to measurement module 2, after distributing, the sample state in sampling needle as shown in figure 7e.
Step 606: the distance piece sample of preset dose is abandoned by sample collection distribution module, after abandoning, the sample state in sampling needle as depicted in fig. 7f.
Step 607: sample collection distribution module distributes sample to measurement module 3, after distributing, the sample state in sampling needle as shown in figure 7g.
Step 608: to all need use measurement module distribute sample after, sampling needle is cleaned, abandons latter end sample simultaneously.
In the sample collection that the present embodiment provides and distribution method, sample collection distribution module once gathers sample to be detected, then the sample of collection is distributed to each measurement module according to predefined procedure.Only carry out a sample collection, collection allocation flow can be had by reduced sample, improve the efficiency of blood testing, sampling needle multiple-contact sample can also be avoided simultaneously, thus bring the problem of pollution.
It will be appreciated by those skilled in the art that, in above-mentioned embodiment, all or part of step of various method can be carried out instruction related hardware by program and completes, this program can be stored in a computer-readable recording medium, and storage medium can comprise: ROM (read-only memory), random access memory, disk or CD etc.
Above content is the further description done the application in conjunction with concrete embodiment, can not assert that the concrete enforcement of the application is confined to these explanations.For the application person of an ordinary skill in the technical field, under the prerequisite not departing from the present application design, some simple deduction or replace can also be made.
Claims (15)
1. a sample analyser, is characterized in that, comprising:
Multiple measurement module, the liquid sample that each measurement module is used for distributing carries out by the measurement obtained for the purpose of different parameters;
Sample collection distribution module, for collecting sample, and distributes to each measurement module by the sample of collection;
Fluid path support module, for each measurement module and sample collection distribution module provide fluid path support;
Control module, it is coupled to sample collection distribution module, once gathering, then the sample of collection being distributed to each measurement module according to predefined procedure for controlling sample collection distribution module to sample to be detected.
2. sample analyser as claimed in claim 1, it is characterized in that, control module also for controlling sample collection distribution module when distributing sample to measurement module, under the liquid level first pin hole of sampling needle being stretched into liquid at the bottom of measurement module, then to injecting sample in measurement module.
3. sample analyser as claimed in claim 2, it is characterized in that, the sample of preset dose in sampling needle, also for controlling sample collection distribution module after distributing sample to previous measurement module, before distributing sample, is abandoned to next measurement module by control module.
4. sample analyser as claimed in claim 3, it is characterized in that, the sample of preset dose in sampling needle also for controlling sample collection distribution module before carrying out first sample distribution, is abandoned by control module.
5. sample analyser as claimed in claim 4, it is characterized in that, control module is also for controlling amount that sample collection distribution module once gathers sample to be detected for the amount sum of the sample that the amount of each measurement module sample and sample collection distribution module are abandoned.
6. sample analyser as claimed in claim 1, is characterized in that, control module also for controlling sample collection distribution module after the measurement module used to all needs distributes sample, is cleaned sampling needle.
7. the method for claim 1, is characterized in that, described multiple measurement module comprises routine blood test count measurement module and C reactive protein measurement module.
8. the sample analyser as described in any one of claim 1-7, is characterized in that, the diameter of the pin hole of described sampling needle is less than the internal diameter of sampling needle needle tubing.
9. the sample analyser as described in any one of claim 1-7, is characterized in that, the needle point of described sampling needle is cone, and the pin hole of described sampling needle is positioned at the sidewall bottom needle tubing.
10. the sample collection of sample analyser and a distribution method, described sample analyser comprises:
Multiple measurement module, the liquid sample that each measurement module is used for distributing carries out by the measurement obtained for the purpose of different parameters;
Sample collection distribution module, for collecting sample, and distributes to each measurement module by the sample of collection;
Fluid path support module, for each measurement module and sample collection distribution module provide fluid path support;
Control module, it is coupled to sample collection distribution module;
It is characterized in that, described method comprises: the sample collection distribution module of sample analyser once gathers sample to be detected, then the sample of collection is distributed to each measurement module according to predefined procedure.
11. methods as claimed in claim 10, it is characterized in that, sample collection distribution module distributes sample to measurement module, is specially: the pin hole of sampling needle first stretches under the liquid level of liquid at the bottom of measurement module by sample collection distribution module, then to injecting sample in measurement module.
12. methods as claimed in claim 11, it is characterized in that, in sample collection distribution module to after previous measurement module distributes sample, before distributing sample to next measurement module, also comprise: the sample of preset dose in sampling needle is abandoned by sample collection distribution module.
13. methods as claimed in claim 12, is characterized in that, before sample collection distribution module carries out first sample distribution, also comprise: the sample of preset dose in sampling needle is abandoned by sample collection distribution module.
14. methods as described in claim 12 or 13, it is characterized in that, in the step that sample collection distribution module once gathers sample to be detected, amount that sample collection distribution module once gathers sample to be detected is the amount sum of the sample that the amount of sample required for each measurement module and sample collection distribution module are abandoned.
15. methods as claimed in claim 10, is characterized in that, after sample collection distribution module distributes sample to the measurement module that all needs use, also comprise: clean the sampling needle of sample collection distribution module.
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| CN109406801A (en) | 2019-03-01 |
| CN105334332B (en) | 2018-10-16 |
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