WO2022104730A1

WO2022104730A1 - Method and apparatus for testing sample absorption of sample analyzer

Info

Publication number: WO2022104730A1
Application number: PCT/CN2020/130565
Authority: WO
Inventors: 王兴红; 邹海涛
Original assignee: 深圳市科曼医疗设备有限公司
Priority date: 2020-11-20
Filing date: 2020-11-20
Publication date: 2022-05-27

Abstract

A method and apparatus for testing sample absorption of a sample analyzer, which method and apparatus relate to the technical field of blood sample testing. The method comprises: a sampling needle entering a WBC pool by means of the movement of a moving component, so as to discharge blood in a head section, and an instrument absorbing a sample; issuing a collection instruction to a hemoglobin concentration measurement apparatus, and reading a liquid voltage signal AD value in the current WBC pool; determining whether the AD value is within a pre-set range; and if the AD value is within the pre-set range, a test instrument executing a test time sequence and outputting a test result. In the method, the instrument cost is not increased; and according to whether an instrument absorbs a sample normally being detected, determination can be made in a timely manner, and a called test time sequence is adjusted, such that unnecessary actions and reagent consumption are prevented, and the working efficiency is improved.

Description

A kind of method and device for detecting sample aspirating by analyzer

technical field

The present invention relates to the technical field of blood sample detection, and in particular, to a method and a device for detecting sample suction by a sample analyzer.

Background technique

In recent years, my country's laboratory medicine business has developed rapidly, and various blood analyzers have become popular in China. It also provides more reliable test indicators for clinical practice, and plays an important role in the diagnosis and identification of diseases. The blood cell analyzer, hence the name Siyi, is an instrument that draws blood samples for analysis, so to ensure the accuracy of the analysis results, the first step is to ensure that the instrument normally draws blood samples from the test tube containing the blood sample.

At present, only a small number of blood cell analyzers provide the function of detecting whether the sample is aspirated, and most of them use photoelectric sensors. By detecting the change of the photoelectric signal passing through the sensor, it is judged whether the sample is aspirated or normal. EN The 107687963 A patent adopts this method. As shown in Figure 1, the sampling syringe is lowered, and enough samples are drawn to make the sample at least exceed the position of the photoelectric sensor assembly set on the light-transmitting pipeline, and then pass the detection signal according to the feedback of the sensor. to determine whether the suction is normal.

technical problem

However, this method has its limitations. As shown in Figure 1, when aspirating the sample, the sampling syringe is pulled down. Under the action of negative pressure, the sample is sucked into the sampling needle, and then passes through the transparent pipeline of the photoelectric sensor. However, since the sampling needle is made of stainless steel, it is opaque to light. If the photoelectric sensor is to function, the sample liquid level must be pulled to the transparent pipe, beyond the sensor position. At present, the general volume of domestic puncture sampling needle is 30ul. If it is to be pulled to the position of the photoelectric sensor, it needs at least 40ul. However, the general detection mode of blood routine five categories plus CRP or SAA blood sample does not need to exceed 30ul, so unless this method is used On a comprehensive instrument that consumes a lot of blood, it is difficult to trigger the photoelectric sensor model.

Therefore, how to provide a method for detecting sample suction by a sample analyzer and a time sequence scheduling method to reduce instrument cost and improve work efficiency has become an urgent technical problem to be solved.

technical solutions

The technical problem to be solved by the present invention is how to provide a method and a device for detecting sample suction by a sample analyzer, so as to reduce the cost of the instrument and improve the work efficiency.

To this end, according to the first aspect, an embodiment of the present invention discloses a method for detecting sample suction by a sample analyzer, including: step a: moving the sampling needle into the sample pool to collect samples in the sample pool; The sampling needle is moved to the detection pool, and the blood of the head is spit out; step c: obtaining the detection value in the current detection pool; step d: judging whether the detection value belongs to the preset range value according to the obtained detection value; step e: if the detection value Within the preset range value, the sample analyzer executes the detection sequence.

According to a second aspect, an embodiment of the present invention discloses a device for detecting sample suction by a sample analyzer, comprising: a sampling module for moving a sampling needle into a sample pool to collect samples in the sample pool; a sample injection module for moving the sampling needle to the detection pool, and spitting out the blood in the head; an acquisition module, used for acquiring the detection value in the current detection pool; a judgment module, used for judging whether the detection value belongs to a preset range value according to the acquired detection value; The detection module is used for the sample analyzer to execute the detection sequence when the detection value is within the preset range value.

beneficial effect

The present invention has the following beneficial effects: compared with the prior art, the embodiment of the present invention moves the sampling needle to the detection cell, spit out the blood in the head, and obtains the detection value of the liquid in the current detection cell through the detection device only in the analyzer ; Determine whether the detection value is within the preset range value; if the detection value is within the preset range value, the sample analyzer executes the detection sequence. The present invention does not increase the cost of the instrument; the components of the sample analyzer itself are used to detect whether the instrument absorbs the sample normally, make a judgment in time, adjust the calling test sequence, save unnecessary actions and reagent consumption, and improve work efficiency.

Description of drawings

In order to illustrate the specific embodiments of the present invention or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the specific embodiments or the prior art. Obviously, the accompanying drawings in the following description The drawings are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without creative efforts.

FIG. 1 is a schematic structural diagram of the existing blood cell analyzer disclosed in this embodiment;

FIG. 2 is a flowchart of a method for detecting a sample aspirating by a sample analyzer disclosed in the present embodiment;

FIG. 3 is a schematic structural diagram of a device for detecting sample suction by a sample analyzer disclosed in this embodiment;

Fig. 4 is the structural representation of blood sample segmentation;

5 is a schematic diagram of the method for detecting hemoglobin concentration disclosed in the present embodiment;

Fig. 6 is the detection and timing call flow chart disclosed in the present embodiment;

FIG. 7 is a schematic diagram of the voltage of the non-spitting blood test disclosed in the present embodiment;

Fig. 8 is the voltage schematic diagram of the spitting 1ul head blood test disclosed in the present embodiment;

Fig. 9 is the voltage schematic diagram of the spitting 2ul head blood test disclosed in the present embodiment;

Fig. 10 is the voltage schematic diagram of the spit 3ul head blood test disclosed in the present embodiment;

FIG. 11 is a schematic diagram of the voltage of the 4ul head blood test disclosed in this embodiment.

BEST MODE FOR CARRYING OUT THE INVENTION

Optionally, the step e includes: the sampling needle moves to the corresponding detection channel according to the time sequence command, completes the specific blood separation action, the sample analyzer performs the detection of each item, and finally outputs the detection result.

Optionally, if the detection value exceeds the preset range value, the sample analyzer calls the cleaning sequence, and cleans the inner and outer walls of the sampling needle and the detection cell through the cleaning sequence, so that the sampling needle and the detection cell are restored to the preparation state before detection.

Optionally, after the cleaning sequence is completed, the actions of step a to step e are performed again.

Optionally, if the second detection value exceeds the preset range value again, the sample analyzer calls the cleaning sequence, and the interface of the sample analyzer pops up a fault prompt box to terminate the detection sequence operation.

Optionally, the detection value is an AD value, the preset range value is an AD preset range value, the detection cell is a wbc cell, and the AD value is collected by a hemoglobin concentration detection device in the sample analyzer.

Optionally, the AD preset range value is 3100-3350.

Optionally, a cleaning module is also included for cleaning the inner and outer walls of the sampling needle and the detection cell when the detection value exceeds the preset range value, so as to restore the sampling needle and the detection cell to the preparation state before detection.

Optionally, it also includes a calling module, which is used to sequentially call the sampling module, the sampling module, the acquisition module, the judgment module and the detection module to work after the sampling needle and the detection cell are restored to the preparation state before detection. If the detected value exceeds the preset range value again, the sample analyzer will call the cleaning module again to work, and the sample analyzer interface will pop up a fault prompt box to terminate the detection sequence operation.

Embodiments of the present invention

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation or a specific orientation. construction and operation, and therefore should not be construed as limiting the invention. Furthermore, the terms "first", "second", and "third" are used for descriptive purposes only and should not be construed to indicate or imply relative importance.

In the description of the present invention, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense, unless otherwise expressly specified and limited, for example, it may be a fixed connection or a detachable connection connection, or integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal connection of two components, which can be a wireless connection or a wired connection connect. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific situations.

In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

The embodiment of the present invention discloses a method for detecting sample suction by a sample analyzer, comprising:

Step a: move the sampling needle into the sample pool, and collect the samples in the sample pool;

Step b: move the sampling needle to the detection pool, and spit out the blood in the head;

Step c: obtain the detection value in the current detection pool;

Step d: according to the acquired detection value, determine whether the detection value belongs to the preset range value;

Step e: If the detection value is within the preset range value, the sample analyzer executes the detection sequence.

It should be noted that, compared with the prior art, in the embodiment of the present invention, the sampling needle is moved to the detection tank, the blood in the head is spit out, and the detection value of the liquid in the current detection tank is obtained through the detection device only in the analyzer; Whether the detection value is within the preset range value; if the detection value is within the preset range value, the sample analyzer executes the detection sequence. The present invention will not increase the cost of the instrument; the components of the sample analyzer itself are used to detect whether the instrument normally absorbs the sample, make a judgment in time, adjust the calling test sequence, save unnecessary actions and reagent consumption, and improve work efficiency.

Specifically, as shown in Figure 2, it includes: S101. The sampling needle enters the wbc pool under the movement of the sampling assembly in the analyzer, and spit out the blood in the head; S102. Issue a collection instruction to the hemoglobin concentration detection device, read The current AD value of the liquid voltage signal in the wbc cell; S103. Determine whether the AD value is within the preset range; S104. If the AD value is within the preset range, the sample analyzer executes the detection sequence. The invention does not increase the cost of the instrument; the hemoglobin concentration detection device itself belongs to the device only carried by the existing analyzer. By detecting whether the instrument normally absorbs the sample, it can make a judgment in time, adjust the calling test sequence, and save unnecessary actions and reagents consumption and improve work efficiency.

Optionally, step e includes: moving the sampling needle to the corresponding detection channel according to the time sequence command to complete the specific blood separation action, the sample analyzer performs the detection of each item, and finally outputs the detection result.

Optionally, the AD preset range value is 3100~3350.

As shown in FIG. 3 , an embodiment of the present invention discloses a device for detecting sample suction by a sample analyzer, including: a sampling module 101 for moving a sampling needle into a sample pool to collect samples in the sample pool; a sampling module 102 , used to move the sampling needle to the detection pool and spit out the blood in the head; the acquisition module 103 is used to acquire the detection value in the current detection pool; the judgment module 104 is used to determine whether the detection value belongs to the preset range according to the acquired detection value The detection module 105 is used for, when the detection value is within the preset range value, the sample analyzer executes the detection sequence.

Optionally, a cleaning module 106 is also included, for cleaning the inner and outer walls of the sampling needle and the detection cell when the detection value exceeds the preset range value, so as to restore the sampling needle and the detection cell to the preparation state before detection.

Optionally, it also includes a calling module 107, which is used to sequentially call the sampling module, the sampling module, the acquisition module, the judgment module and the detection module to work after the sampling needle and the detection cell are restored to the preparation state before detection. If the second detection value exceeds the preset range value again, the sample analyzer will call the cleaning module again to work, and the interface of the sample analyzer will pop up a fault prompt box to terminate the detection sequence operation.

As shown in Figure 4, a is the head blood, and the syringe eliminates the stroke difference from suction to discharge by throwing the head blood; then the sample blood volume required for wbc counting; then throw away the blood sample b to avoid channel crossing Contamination, then the blood volume required for crp counting; then throw away the blood sample of part c to avoid cross-contamination of the channel; then the blood sample required for diff classification; and finally the tail blood d is cleaned and removed together with the inside of the cleaning sampling needle.

The blood thrown away by the normal instrument, such as a, b, c, and d in Figure 4, is recovered inside the swab, collected and discharged under the negative pressure of the waste liquid module connected to the swab assembly.

The method for detecting whether the instrument is normally aspirating the sample provided by this application is to divide the blood from the head into the wbc pool instead of throwing it in the swab, and then test the background voltage at this time through the device for detecting the concentration of hemoglobin in the instrument itself. Whether it is within the preset range to judge whether the suction is normal.

The present application will be described in detail below with specific embodiments.

As shown in Figure 5, the hemoglobin concentration is measured by colorimetry. At one end of the detection cell, the LED light tube emits light with a center wavelength of 525 nm to illuminate the solution in the detection cell, and the other end receives the projected light through the photocell, and then the light signal is converted into a voltage. The signal is compared with the background voltage signal tested at the beginning of the detection cell (the detection cell only has diluent at the beginning, and the transmitted light signal is the strongest), and the concentration of the sample hemoglobin is calculated.

FIG. 6 is a flow chart of detection and timing invocation disclosed in this embodiment.

The range of AD value is obtained through many experiments. Figure 7 is the voltage of the test without spitting blood in the head; Figure 8 is the voltage in the test of vomiting 1ul blood in the head; Figure 9 is the voltage in the test with blood in the head of 2ul; 10 is the voltage of spit 3ul head blood test; Figure 11 is the voltage of 4ul head blood test.

Combined with the test data, and considering that the acquisition voltage is instantaneously completed within 250ms, and the instrument motor injector is always working during the signal acquisition, there is a vibration of individual instruments that causes the liquid level to fluctuate and thus the voltage AD value. There will be a slight deviation in the blood volume, so set the AD value within 3100~3350, which means the blood separation is normal, that is, the instrument suction is normal. Of course, the embodiments of the present application only provide a way, and a more accurate AD value can be obtained by using a higher-precision measurement and detection device.

Working principle: Compared with the prior art, in the embodiment of the present invention, the sampling needle enters the wbc pool under the movement of the sampling assembly in the analyzer, and spit out the blood in the head; Take the AD value of the liquid voltage signal in the current wbc cell; determine whether the AD value is within the preset range; if the AD value is within the preset range, the sample analyzer executes the detection sequence. The present invention does not increase the cost of the instrument; by detecting whether the instrument normally draws samples, judgment is made in time, the calling test sequence is adjusted, unnecessary actions and reagent consumption are saved, and work efficiency is improved.

In another embodiment, the head blood can be spit into the crp counting pool, and the AD value in the crp pool can be detected by the crp detection device in the sample analyzer to determine whether the AD value is within the preset range; Within the set range, the sample analyzer executes the detection sequence, that is, through the sequence command, the samples in the sampling needle are sequentially injected into the crp pool, the wbc pool and the diff classification pool for detection.

Obviously, the above-mentioned embodiments are only examples for clear description, and are not intended to limit the implementation manner. For those of ordinary skill in the art, changes or modifications in other different forms can also be made on the basis of the above description. There is no need and cannot be exhaustive of all implementations here. And the obvious changes or changes derived from this are still within the protection scope of the present invention.

Industrial Applicability

The present application has industrial applicability.

Claims

A method for detecting sample suction by a sample analyzer, comprising:

Step a: move the sampling needle into the sample pool, and collect the samples in the sample pool;

Step b: move the sampling needle to the detection pool, and spit out the blood in the head;

Step c: obtain the detection value in the current detection pool;

Step d: according to the acquired detection value, determine whether the detection value belongs to the preset range value;

Step e: If the detection value is within the preset range value, the sample analyzer executes the detection sequence.
The method for detecting sample suction by a sample analyzer according to claim 1, wherein the step e comprises:

The sampling needle moves to the corresponding detection channel according to the sequence command, completes the specific blood separation action, the sample analyzer performs the detection of each item, and finally outputs the detection result.
The method according to claim 1, wherein if the detected value exceeds the preset range value, the sample analyzer calls the cleaning sequence, and cleans the inner and outer walls of the sampling needle and the detection pool through the cleaning sequence. , to restore the sampling needle and detection cell to the preparation state before detection.
The method according to claim 3, characterized in that, after the cleaning sequence is completed, the actions of step a to step e are performed again.
The method for detecting sample suction by a sample analyzer according to claim 4, wherein if the second detected value exceeds the preset range value again, the sample analyzer invokes the cleaning sequence, and a fault prompt pops up on the sample analyzer interface box to terminate the detection sequence operation.
The method according to any one of claims 1-5, wherein the detection value is an AD value, the preset range value is an AD preset range value, and the detection cell is an AD value. For the wbc pool, the AD value is collected by the hemoglobin concentration detection device in the sample analyzer.
The method according to claim 6, wherein the AD preset range value is 3100-3350.
A device for detecting sample suction of a sample analyzer, characterized in that it comprises:

The sampling module is used to move the sampling needle into the sample pool and collect the samples in the sample pool;

a sample injection module, used to move the sampling needle to the detection cell and spit out the blood in the head segment;

The acquisition module is used to acquire the detection value in the current detection pool;

a judgment module, used for judging whether the detection value belongs to the preset range value according to the obtained detection value;

The detection module is used for the sample analyzer to execute the detection sequence when the detection value is within the preset range value.
The device for detecting sample suction by an analyzer of claim 8, further comprising a cleaning module for cleaning the inner and outer walls of the sampling needle and the detection pool when the detected value exceeds the preset range value, so that the The sampling needle and detection cell are restored to the pre-test preparation state.
The device for sampling aspirating by a detection sample analyzer according to claim 9, further comprising a calling module for sequentially calling the sampling module and the sampling module after the sampling needle and the detection cell are restored to the preparation state before detection , the acquisition module, the judgment module and the detection module work. If the second detection value exceeds the preset range value again, the sample analyzer will call the cleaning module again to work, and the sample analyzer interface will pop up a fault prompt box to terminate the detection sequence. operate.