CN203798733U - POCT (Point of Care Testing) specific protein analysis system - Google Patents
POCT (Point of Care Testing) specific protein analysis system Download PDFInfo
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- CN203798733U CN203798733U CN201420144229.8U CN201420144229U CN203798733U CN 203798733 U CN203798733 U CN 203798733U CN 201420144229 U CN201420144229 U CN 201420144229U CN 203798733 U CN203798733 U CN 203798733U
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- specific protein
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- poct
- light path
- detector tube
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 46
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 46
- 238000012123 point-of-care testing Methods 0.000 title claims abstract description 37
- 238000004458 analytical method Methods 0.000 title abstract description 5
- 238000006243 chemical reaction Methods 0.000 claims abstract description 51
- 210000004369 blood Anatomy 0.000 claims abstract description 28
- 239000008280 blood Substances 0.000 claims abstract description 27
- 238000001514 detection method Methods 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 10
- 230000008569 process Effects 0.000 claims abstract description 9
- 238000005534 hematocrit Methods 0.000 claims abstract description 3
- 102100032752 C-reactive protein Human genes 0.000 claims description 27
- 238000003756 stirring Methods 0.000 claims description 27
- 239000000376 reactant Substances 0.000 claims description 24
- 238000012360 testing method Methods 0.000 claims description 23
- 108010074051 C-Reactive Protein Proteins 0.000 claims description 14
- 230000035484 reaction time Effects 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 2
- 230000005540 biological transmission Effects 0.000 abstract description 7
- 238000003745 diagnosis Methods 0.000 abstract description 3
- 208000015181 infectious disease Diseases 0.000 abstract 2
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000002331 protein detection Methods 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 description 16
- 230000000694 effects Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 102000017011 Glycated Hemoglobin A Human genes 0.000 description 1
- 108010014663 Glycated Hemoglobin A Proteins 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004397 blinking Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 239000003219 hemolytic agent Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000003541 multi-stage reaction Methods 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000001141 propulsive effect Effects 0.000 description 1
- 230000018612 quorum sensing Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a POCT (Point of Care Testing) specific protein analysis system, which is used for determining the content of C reaction protein, namely CRP in whole human blood. The POCT specific protein analysis system comprises a plurality of detection units and a control system for automatically controlling the C reaction protein detection process in whole human blood, and each detection unit consists of a reaction station, an incident light path system, a transmission light path system and a scattering light path system. The transmission light path system and the scattering light path system which are arranged are used for respectively detecting the hematocrit and the concentration of C reaction protein in the whole blood, the detection process is convenient and simple, the errors existing in the complicated operation in the prior art can be decreased, a plurality of samples can be detected simultaneously, the POCT specific protein analysis system is simple in volume, high in sensitivity, and reliable and accurate in detection result, and can be widely used for evaluating clinical fast diagnosis infection and infection afterhealing of large and medium-sized and small-sized hospitals and outpatient departments.
Description
Technical field
The utility model relates to a kind of POCT specific protein analytic system.
Background technology
POCT (point of care testing) is called instant detection, be a kind of volume little, be convenient for carrying, can be on-the-spot to system that in people's whole blood, c reactive protein detects.Glycosylated hemoglobin, high-sensitive C-reactive protein etc. are effective test items of a lot of chronic diseases, common disease, frequently-occurring disease, have recently occurred the detecting instrument that some are small-sized, make the detection simple and convenient more of c reactive protein in whole blood.
Chinese patent CN102478519B discloses a kind of specific protein analytic system, it comprises at least one composite reaction cup, a combined heated system, the self-propelled machine structure of reagent more than one and a transmission scattering combined optical path system, manually reagent is put into the system after station of detecting automatically complete heat, the overall process of stirring, haemolysis, the time-sharing automatic interpolation of many reagent, test reaction curve, result of calculation value and printing.When this specific protein analytic system detects, first draw quantitative hemolytic agent and put into reaction cup, the blood sample of the quantitative collection tester third finger splashes into reaction cup again, the socket of reaction cup and honeycomb fashion reagent bottle is combined and put into reaction position, then by reagent propulsive mechanism, suitable ratio color bits is delivered to in reaction position and carry out the detection of specific protein.
Above-mentioned specific protein analytic system can realize the robotization of step except sample of blood, drawn, but this system is applicable to many reagent and measures specific protein, and need through self-propelled machine structure, reaction reagent to be delivered to successively than color bits, can only detect a sample at every turn.So, be necessary to design a kind of single reaction reagent, operation of being applicable to easier and can detect the specific protein analytic system of multiple samples simultaneously.
Utility model content
In order to overcome the problems referred to above, the utility model discloses a kind of POCT specific protein analytic system, be the content of CRP for measuring people's whole blood c reactive protein, this system is applicable to single reagent CRP reactant liquor, has easy and simple to handle, highly sensitive, reproducible, low cost and other advantages.
The concrete technical scheme that the utility model adopts is to provide a kind of POCT specific protein analytic system, be the content of CRP for measuring people's whole blood c reactive protein, it is characterized in that, described POCT specific protein analytic system comprises some detecting units and for realizing the control system of the automatic control to people's whole blood c reactive protein testing process, described detecting unit comprises:
A reaction station, for placing the detector tube that is preinstalled with CRP reactant liquor;
Input path system, comprises incident light source, and described incident light source is corresponding with described reaction station;
Transmitted light path system, comprises transmitted light receiver and is arranged at the green color filter of described transmitted light receiver front portion, for identifying the adding of people's whole blood, start detection and detecting the erythrocytic hematocrit of whole blood;
Scattering light path system, comprises scattered light receiver, for detection of the extent of reaction of reactant liquor in described detector tube and detect the concentration of c reactive protein.
Further, described POCT specific protein analytic system is also provided with automaton, described automaton is connected with described transmitted light path system signal, when have whole blood add detector tube time, described transmitted light path system is to automaton transmitted signal, and automaton starts corresponding detecting unit and starts testing.
Further, described automaton is provided with timer, described reaction timer is for setting the reaction time of corresponding detecting unit, in the time having whole blood to add detector tube, described reaction timer starts timing, timing arrives when setting-up time, and described automaton stops the testing of this corresponding unit, and carries out with one or several in issuing orders: show, print, storage or transmit the testing result of corresponding unit.
Further, described incident light system, transmitted light path system and scattering light path system also arrange respectively incident light through hole, transmitted light through hole and scattered light through hole.
Further, described POCT specific protein analytic system also comprises reactant liquor stirring apparatus, described reactant liquor stirring apparatus comprises the magnetic stir bar that is placed in detector tube, stir timer and be arranged at the electromagnet of detector tube outside, and described electromagnet and described stirring timer are electrically connected with described control system.
Further, described detector tube below arranges detector tube inductor, for responding to packing into and signal being transferred to described control system of detector tube.
Further, described scattering light path system and described transmitted light path system are 15～25 degree angles.
Further, described input path system is single input path, and the wavelength of described single input path is 500～800 nanometers.
Further, the wavelength-filtered of described green color filter is 500～600 nanometers.
Further, the wavelength-filtered of described green color filter is 540～570 nanometers.
Further, described POCT specific protein analytic system also comprises a housing, and described several detecting units and set-up of control system are in described housing.
Further, described housing arranges LCDs, and described LCDs is used for showing actions menu, inquiry test result.
The utility model compared with prior art has significant progress and advantage, pass through technique scheme, adopt single reagent CRP reactant liquor as reaction reagent, transmission scattering double beam system is set to be detected the concentration of packed cell volume in whole blood and c reactive protein respectively, overall process is realized and is added the robotization control step except reaction reagent and whole blood by control system, realizing robotization stirs, timing and detection, testing process is convenient and simple, can reduce the error that in prior art, troublesome operation exists, specific protein reactive system of the present utility model can detect several different samples simultaneously in addition, greatly improve the service efficiency of machine, reduce the time that user waits for testing result, and specific protein reactive system volume of the present utility model is little, simple in structure, highly sensitive, testing result is reliably accurate, can be widely used for large, middle and small hospital, evaluation after the clinical quick diagnosis of outpatient service infects and infect more.
Brief description of the drawings
In order to be illustrated more clearly in the technical scheme in the utility model embodiment, below the accompanying drawing of required use during embodiment is described is briefly described, apparently, accompanying drawing in the following describes is only embodiment more of the present utility model, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the structural representation of a detecting unit embodiment 1 of the utility model;
Fig. 2 is the structural representation of a detecting unit embodiment 4 of the utility model;
Fig. 3 is the structural representation of a detecting unit embodiment 5 of the utility model;
Fig. 4 is the structural representation of reactant liquor stirring apparatus in the utility model;
Fig. 5 is the schematic appearance of a kind of POCT specific protein of the utility model analysis system.
Wherein, 1, reaction station; 2, incident light source; 21, incident light through hole; 3, electromagnet; 31, magnetic stir bar; 4, transmitted light receiver; 41, transmitted light through hole; 42, green color filter; 5, scattered light receiver; 51, scattered light through hole; 6; Base; 7, housing; 71, LCDs; 72, reaction cabin; 8, control device
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail.According to following explanation and claims, advantage of the present utility model and feature will be clearer, it should be noted that, accompanying drawing all adopts form and the non-ratio accurately of simplification, only for convenient, the object of aid illustration the utility model embodiment lucidly.
The structural representation that is illustrated in figure 1 the embodiment 1 of a detecting unit in the utility model, this detecting unit is arranged on base 6, comprises a reaction station 1, input path system, transmitted light path system, scattering light path system; Reaction station 1 is arranged on the middle part of base 6, and input path system is arranged on a side of base 6, comprises incident light source 2, incident light source 2 is LED light emitting diode, be fixedly installed on base 6, the outer setting outer cover of incident light source 2, incident light wavelength is 500～800 nanometers; Transmitted light path system and scattering light path system are arranged on the opposite side of base, transmitted light path system comprises transmitted light receiver 4, green color filter 42, the front of transmission receiver 4 arranges green color filter 42, the wavelength-filtered of green color filter 42 is 500～600 nanometers, the wavelength-filtered of preferred green color filter 42 is 540～570 nanometers, green color filter 42 passes through ruddiness for filtering green glow, be convenient to transmitted light receiver 4 and receive ruddiness, transmitted light receiver 4 receives ruddiness, and control system just starts to calculate packed cell volume; Scattering light path system comprises scattered light receiver 5, and when scattered light receiver 5 receives scattering light source, control system starts to detect the concentration of c reactive protein.
In order further to optimize the automatic control effect of a kind of POCT specific protein of the utility model analytic system, in the second embodiment of utility model, POCT specific protein analytic system is also provided with automaton, this automaton is connected with transmitted light path system signal, in the time having whole blood to add detector tube, transmitted light path system is to automaton transmitted signal, and automaton starts corresponding detecting unit and starts testing.
In order further to optimize the automatic control effect of a kind of POCT specific protein of the utility model analytic system, in the third embodiment of the present utility model, be provided with reaction timer at the automaton of the second embodiment of the present utility model, described reaction timer is for setting the reaction time of corresponding detecting unit, when have whole blood add detector tube time, described reaction timer starts timing, when timing arrives setting-up time, described automaton stops the testing of this corresponding unit, and carry out with one or several in issuing orders: show, print, storage, or the testing result of transmission corresponding unit.
Detect effect for fear of light disturbing effect between each detecting unit of a kind of POCT specific protein of the utility model analytic system, as shown in Figure 2, in the 4th kind of embodiment of the present utility model, in input path system, incident light through hole 21 is set, incident light source arrives reaction station by incident light through hole; Transmitted light path system arranges transmitted light through hole 41, and reaction station 1 connects transmitted light receiver 4 through transmitted light through hole 41; Scattering light path system arranges scattered light through hole 51, and scattered light through hole 51 is arranged on counterclockwise 15～25 degree directions of transmitted light through hole 41, and reaction station 1 connects scattered light receiver 5 through scattered light through hole 51.For the light further reducing between each detecting unit disturbs, also can between each detecting unit, light barrier be set.
In order to realize the full and uniform mixing of reactant liquor in detector tube in a kind of POCT specific protein of the utility model analytic system, as shown in Figure 3, in the 5th kind of embodiment of the present utility model, reactant liquor stirring apparatus is set, the below of reaction station 1 arranges detector tube inductor (not shown), for responding to the position of detector tube and signal being transferred to control system, control system starts reactant liquor stirring apparatus after receiving signal, as shown in Figure 4, this device comprises the magnetic stir bar 31 being placed in detector tube, stir timer and the electromagnet 3 that is arranged at detector tube outside, electromagnet 3 and stirring timer are electrically connected with control system 8, the signal that receives detector tube inductor when control system 8 starts electromagnet 3 and stirs timer, under the effect of electromagnet 3, the magnetic stir bar 31 of detector tube moves up and down in reactant liquor, reactant liquor is stirred, after stirring 5～10s, stirring apparatus quits work.
Carry out, when c reactive protein detects, the detector tube of splendid attire single reagent CRP reactant liquor is positioned over to reaction position upper, the startup of reactant liquor stirring apparatus mixes reactant liquor, and the light that input path system incident light source sends arrives detector tube through incident light through hole; In detector tube, splash into a certain amount of people's whole blood, the color of reactant liquor starts to redden, transmitted light is transmitted optical receiver through green color filter and receives, start reaction timer, start to calculate packed cell volume simultaneously, and scattered light is scattered optical receiver reception through scattered light through hole, control system starts to detect the concentration of CRP albumen, after 30～900s, detect and stop, detector tube can being taken out, carry out the detection of next sample.
A detecting unit of the present utility model matches with control system and can complete fast and accurately the detection of CRP albumen, in order to be convenient for carrying, 1～5 detecting unit and control system to be accommodated in and in a housing, just to form a kind of POCT specific protein of the utility model analytic system; If use and more detecting units can be set in specific protein analytic system in laboratory, the number of detecting unit can and need to arrange according to use occasion, does not limit herein.Multiple detecting units are set and can detect several different samples simultaneously, greatly improve the service efficiency of machine, minimizing user waits for the time of testing result, as shown in Figure 5, housing 7 arranges some reaction cabins 72, and the position of reaction cabin 72 is corresponding with detecting unit reaction work bit position; Housing 7 arranges LCDs 71 simultaneously, for showing actions menu and inquiry test result.
The operation steps of a kind of POCT specific protein of the utility model analytic system is as follows:
Step 1, start preheating, makes system reach working temperature;
Step 2, the interior blinking red lamp of screen sash that reaction cabin is corresponding, shows that this reaction station is ready, can detect;
Step 3, is placed in detector tube in the reaction station of reaction cabin, and signal is transferred to control system by detector tube inductor, and the interior green light flicker of screen sash that reaction cabin is corresponding, starts reactant liquor stirring apparatus simultaneously and stir timer, mixes the reactant liquor in reaction cup.
Step 4, draw 10 microlitre whole bloods with kapillary, add in the detector tube mixing, the variation (reddening) of transmitted light receiver Quorum Sensing liquid color, start reaction timer, the control system content of haemoglobin that automatically converts, and convert packed cell volume to, converse the actual amount that adds serum in reaction cup.
Step 5, reactant liquor stirring apparatus stopped afterwards automatically at 5～10 seconds, and the green light in corresponding screen sash stops flicker, the concentration of scattering light path system start detection CRP.
Step 6, reacted after 30～900 seconds, the green light lamp that reddens in screen sash, and optical system stops measuring, and in the concentration of liquid crystal display screen display CRP.
Step 7, takes out and detects complete detector tube cup, and now in screen sash, red light starts flicker again, and expression can be carried out the mensuration of next sample.
The utility model compared with prior art has significant progress and advantage, adopt single reagent CRP reactant liquor as reaction reagent, transmission scattering double beam system is set to be detected the concentration of packed cell volume in whole blood and c reactive protein respectively, overall process is realized and is added the robotization control step except reaction reagent and whole blood by control system, testing process is convenient and simple, can reduce the error that in prior art, troublesome operation exists, specific protein reactive system of the present utility model can detect several different samples simultaneously in addition, greatly improve the service efficiency of machine, reduce the time that user waits for testing result, and specific protein reactive system volume of the present utility model is little, simple in structure, highly sensitive, testing result is reliably accurate, can be widely used for large, middle and small hospital, the clinical quick diagnosis of outpatient service infects and infects the evaluation of prognosis.
Above-described specific embodiment; the purpose of this utility model, technical scheme and beneficial effect are further described; institute is understood that; the foregoing is only specific embodiment of the utility model; be not limited to the utility model; all within spirit of the present utility model and principle, any amendment of making, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.
Claims (10)
1. a POCT specific protein analytic system, be the content of CRP for measuring people's whole blood c reactive protein, it is characterized in that, described POCT specific protein analytic system comprises at least one detecting unit and for realizing the control system of the automatic control to people's whole blood c reactive protein testing process, described detecting unit comprises:
A reaction station, for placing the detector tube that is preinstalled with CRP reactant liquor;
Input path system, comprises incident light source, and described incident light source is corresponding with described reaction station;
Transmitted light path system, comprises transmitted light receiver and is arranged at the green color filter of described transmitted light receiver front portion, for identifying the adding of people's whole blood, start detection and detecting the erythrocytic hematocrit of whole blood;
Scattering light path system, comprises scattered light receiver, for detection of the extent of reaction of reactant liquor in described detector tube the concentration of testing c reactive protein.
2. POCT specific protein analytic system according to claim 1, it is characterized in that: described POCT specific protein analytic system is also provided with automaton, described automaton is connected with described transmitted light path system signal, in the time having whole blood to add detector tube, described transmitted light path system is to automaton transmitted signal, and automaton starts corresponding detecting unit and starts testing.
3. POCT specific protein analytic system according to claim 2, it is characterized in that: described automaton is provided with reaction timer, described reaction timer is for setting the reaction time of corresponding detecting unit, in the time having whole blood to add detector tube, described reaction timer starts timing, when timing arrives setting-up time, described automaton stops the testing of this corresponding unit, and carries out with one or several in issuing orders: show, print, storage or transmit the testing result of corresponding unit.
4. POCT specific protein analytic system according to claim 3, is characterized in that: described incident light system, transmitted light path system and scattering light path system also arrange respectively incident light through hole, transmitted light through hole and scattered light through hole.
5. POCT specific protein analytic system according to claim 4, it is characterized in that: described detecting unit also comprises reactant liquor stirring apparatus, described reactant liquor stirring apparatus comprises the magnetic stir bar that is placed in detector tube, stir timer and be arranged at the electromagnet of detector tube outside, and described electromagnet and described stirring timer are electrically connected with described control system.
6. POCT specific protein analytic system according to claim 5, is characterized in that: described detector tube below arranges detector tube inductor, for responding to packing into and signal being transferred to described control system of detector tube.
7. want the POCT specific protein analytic system described in 6 according to right, it is characterized in that: described scattering light path system and described transmitted light path system are 15～25 degree angles.
8. POCT specific protein analytic system according to claim 7, is characterized in that: described input path system is single input path, the wavelength of described single input path is 500～800 nanometers.
9. POCT specific protein analytic system according to claim 8, is characterized in that: the wavelength-filtered of described green color filter is 500～600 nanometers.
10. POCT specific protein analytic system according to claim 1, is characterized in that: described POCT specific protein analytic system also comprises a housing, and described detecting unit and set-up of control system are in described housing.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420144229.8U CN203798733U (en) | 2014-03-27 | 2014-03-27 | POCT (Point of Care Testing) specific protein analysis system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420144229.8U CN203798733U (en) | 2014-03-27 | 2014-03-27 | POCT (Point of Care Testing) specific protein analysis system |
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| CN203798733U true CN203798733U (en) | 2014-08-27 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483237A (en) * | 2015-01-06 | 2015-04-01 | 陈玉梅 | Multi-sample blood pressure product measuring method and device based on transmissivity changing |
| CN104949942A (en) * | 2014-03-27 | 2015-09-30 | 苏州德沃生物技术有限公司 | POCT (point-of-care testing) special protein analysis system |
-
2014
- 2014-03-27 CN CN201420144229.8U patent/CN203798733U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104949942A (en) * | 2014-03-27 | 2015-09-30 | 苏州德沃生物技术有限公司 | POCT (point-of-care testing) special protein analysis system |
| CN104483237A (en) * | 2015-01-06 | 2015-04-01 | 陈玉梅 | Multi-sample blood pressure product measuring method and device based on transmissivity changing |
| CN104483237B (en) * | 2015-01-06 | 2017-02-22 | 陈玉梅 | Multi-sample blood pressure product measuring method and device based on transmissivity changing |
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