CN106501539A - A kind of sampling system and sample injection method for reducing dead volume liquid - Google Patents
A kind of sampling system and sample injection method for reducing dead volume liquid Download PDFInfo
- Publication number
- CN106501539A CN106501539A CN201611115962.7A CN201611115962A CN106501539A CN 106501539 A CN106501539 A CN 106501539A CN 201611115962 A CN201611115962 A CN 201611115962A CN 106501539 A CN106501539 A CN 106501539A
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- Prior art keywords
- pump
- sample introduction
- pipeline
- dead volume
- liquid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
Abstract
The invention discloses a kind of sampling system and sample injection method for reducing dead volume liquid, which mainly includes sample introduction pipeline, the sample introduction pipeline sample introduction end is provided with three-way valve, the another two port of the three-way valve is respectively feed liquor port and air inlet port, also include the first pump and the second pump, input duct and drain pipe road is connected with first pump, the drain pipe road and the connection of feed liquor port, second pump includes admission line and outlet pipe, the outlet pipe is connected with air inlet port, the present invention is not on the premise of sample introduction circuit design is affected, the impact for reducing " dead volume " to greatest extent by the means for aiding in sample introduction, achieve the noresidue sample introduction of whole fluids, so that this system can be widely applied to the fields such as analysis test.
Description
Technical field
The present invention relates to a kind of sampling system, more particularly to a kind of sampling system for reducing dead volume liquid and enter sample prescription
Method.
Background technology
Due to the presence of infusion channel in test system, " dead volume " phenomenon, i.e. input test in existing test, occurs
The fluid of passage does not have all through sample, and some is remained in fluid transfer, causes tester to produce mistake
Sentence so that deviation occurs in test result." dead volume " is to test a difficult problem for not obtaining effectively solving in industry at present.
Traditional method shortens duct length, reduces pipe diameter etc. using the volume of transmission channel is reduced reducing " dead volume ", such as
Deng, but this method can bring the difficulty of the complication and processing of structure, while reducing the motility of sample introduction, increase operation difficulty.
As Chinese patent 201010581460.X discloses a kind of column sample injection device without dead volume effect, it is related to one kind
Liquid sample introduction device.There is provided a kind of for fluid sample, without dead volume effect, be suitable for micrometeor clastotype, can letter
Just the column sample injection device without dead volume effect of sampling volume is controlled.The device has simple structure, can pass through Automatic Program
The features such as control sample introduction process and high experimental precision.It is provided with source of the gas, the 1st electromagnetic valve, liquid containing bottle, valve, detached dowel and the 2nd electromagnetism
Valve；The outlet of source of the gas connects liquid containing bottle entrance through the 1st electromagnetic valve, and liquid containing bottle outlet is electric with detached dowel one end and the 2nd respectively through valve
Magnet valve connects, and the external detector of the other end of detached dowel, the outlet of the 2nd electromagnetic valve connect waste liquid discharge pipe, and this kind of structure is each
It is required for excluding unnecessary sample after sampling, so as to cause the waste of experimental liquid.
Content of the invention
Present invention solves the technical problem that being to provide a kind of a kind of sample introduction of the reduction dead volume liquid that can reduce dead volume
System.
The technical solution adopted for the present invention to solve the technical problems is：A kind of sampling system for reducing dead volume liquid,
Including sample introduction pipeline, the sample introduction pipeline sample introduction end is provided with three-way valve, and the another two port of the three-way valve is respectively feed liquor
Port and air inlet port, also include the first pump and the second pump, are connected with input duct and drain pipe road on first pump, described
Drain pipe road and the connection of feed liquor port, second pump include that admission line and outlet pipe, the outlet pipe are led to air inlet
Mouth connection.
It is further：First pump is duplex plunger pump.
It is further：First pump includes two single plunger pumps, the drain pipe road of described two single plunger pumps
Connect with feed liquor port.
The invention also discloses a kind of reduce dead volume Liquid sample introduction method, including：
A, three-way valve is switched to the second pump, the second pump blows air into sample introduction pipeline, and sample introduction pipeline is purged；
B, three-way valve is switched to the first pump, sample introduction pipeline connects sample introduction object, the first pump is according to the speed for setting to entering
Liquid needed for the injection of sample pipeline；
C, three-way valve is switched to the second pump, gas is passed through in sample introduction pipeline by the second pump according to the speed for setting, will pipe
Liquid in road pushes sample introduction object.
It is further：First pump be duplex plunger pump or two drain pipe roads and the single plunger that connects of feed liquor port
Pump；
In step B, required liquid is caused uninterruptedly to sample introduction pipeline according to setting by duplex plunger pump or two single plunger pumps
Fixed speed injection.
The invention has the beneficial effects as follows：The present invention on the premise of do not affect sample introduction circuit design, by aiding in sample introduction
Means reduce the impact of " dead volume " to greatest extent, it is achieved that the noresidue sample introduction of whole fluids so that this system can be extensive
Be applied to analysis test etc. field.
Description of the drawings
Fig. 1 is step A schematic diagram.
Fig. 2 is step B schematic diagram.
Fig. 3 is step C schematic diagram.
Fig. 4 is 1 schematic diagram of embodiment.
Fig. 5 is 2 schematic diagram of embodiment.
Fig. 6 is 3 front view of embodiment.
Fig. 7 is 3 top view of embodiment.
It is labeled as in figure：Sample introduction pipeline 1, three-way valve 2, the first pump 3, the second pump 4, input duct 5, drain pipe road 6, air inlet
Pipeline 7, outlet pipe 8.
Specific embodiment
The present invention is further described with reference to the accompanying drawings and detailed description.
A kind of reduction dead volume Liquid sample introduction system as shown in Figure 1 to Figure 3, including sample introduction pipeline 1, the sample introduction pipeline
1 sample introduction end is provided with three-way valve 2, and the another two port of the three-way valve 2 is respectively feed liquor port and air inlet port, also includes the
One pump 3 and the second pump 4, are connected with input duct 5 and drain pipe road 6 on first pump 3, the drain pipe road 6 and feed liquor are logical
Mouth connection, second pump 4 include that admission line 7 and outlet pipe 8, the outlet pipe 8 are connected with air inlet port；
First pump and the second pump can be the pump housing of the transmission fluid such as plunger displacement pump, dosing pump；
The input duct 5 of first pump 3 connects extraneous liquid storage cylinder, and the sample introduction pipeline 1 connects outside sample introduction object, institute
Stating admission line 7 and connecting extraneous gas receiver, the gas for using is the gas that adverse effect is not produced to sample introduction target, such as lazy
Property gas etc., during work, first using the second pump 4 according to the injection speed and injection volume for setting to 1 injected gas of sample introduction pipeline,
Exclude the excessive gas in sample introduction pipeline 1,2 direction of reconvert three-way valve, the sample size and sample introduction for liquid being controlled using the first pump 3
Speed is passed through liquid in sample introduction pipeline 1, so that liquid is entered in outside sample introduction object, after the completion of feed liquor, adjusts threeway
Gas is passed through sample introduction pipeline 1 by the direction of valve 2, the second pump 4, and the residual liquid in input duct 5 is all pushed feed liquor object
In, the purpose of accurate feed liquor is reached, is greatly reduced due to transmission pipeline and is produced the impact of " dead volume ".
Additionally, first pump 3 is duplex plunger pump or two single plunger pumps, the drain pipe road of described two single plunger pumps is equal
Connect with feed liquor port, when sample size is more than single pump capacity and during needs long-time continuous sample introduction, can cause duplex plunger pump or
Two single plunger pump interval work, are endlessly passed through liquid to water influent pipeline, realize long-time continuous sample introduction.
The invention also discloses a kind of reduce dead volume Liquid sample introduction method, including：
A, three-way valve 2 is switched to the second pump 4, the second pump 4 blows air into sample introduction pipeline 1, sample introduction pipeline 1 is blown
Sweep；
B, three-way valve 2 is switched to the first pump 3, sample introduction pipeline 1 connects sample introduction object, and the first pump 3 is according to the speed for setting
Required liquid is injected to sample introduction pipeline 1；
C, three-way valve 2 is switched to the second pump 4, gas is passed through in sample introduction pipeline 1 by the second pump 4 according to the speed for setting,
Liquid in pipeline is pushed sample introduction object.
Additionally, first pump is duplex plunger pump or two single plunger pumps；
In step B, required liquid is caused uninterruptedly to sample introduction pipeline according to setting by duplex plunger pump or two single plunger pumps
Fixed speed injection.
It is below several embodiments of the method：
Embodiment 1：
When sample size is more than sample introduction 1 pipe capacity of pipeline, first the second pump 4 outwardly gas receiver pumping, 2 turns of three-way valve
To air inlet port is connected, and the second plunger valve outwardly extracts liquid to water influent pipeline ventilation purging, the first pump 3, then three-way valve
2 turn to, and feed liquor port is connected, and the first pump 3 carries out feed liquor operation to sample introduction pipeline 1, and the liquid in sample introduction pipeline 1 enters sample introduction pair
As after the first pump 3 completes quantitative liquid-filling, three-way valve 2 is turned to, and air inlet port is connected, and the second plunger valve is ventilated to water influent pipeline
The dead volume hydrops of residual is all pushed sample introduction object by purging.
Embodiment 2：
When sample size is less than or equal to sample introduction 1 pipe capacity of pipeline, first the second pump 4 outwardly gas receiver pumping, three-way valve 2
Turn to, air inlet port is connected, the second pump outwardly extracts liquid to water influent pipeline ventilation purging, the first pump 3, then three-way valve 2
Turn to, feed liquor port is connected, the first pump 3 carries out quantitative feed liquor operation to sample introduction pipeline 1, after the completion of, three-way valve 2 is turned to, air inlet
Port is connected, and the second pump is blown to water influent pipeline and be pushed into water influent pipeline outlet by liquid, and system carries out beginning sample introduction and points out, and second
Pump 4 changes draft speed carries out constant speed ventilation to water influent pipeline, and gas constant speed all pushes liquid by sample introduction object.
The system and the first pump, the connection of the second pump, can judge liquid by the method for quantitatively injection gas and be pushed into
Water influent pipeline export, or water influent pipeline exit install pressure transducer or photoelectric sensor etc. come judge liquid be pushed into into
Liquid pipe way outlet.
Embodiment 3：
When sample size is more than single pump capacity and needs long-time continuous sample introduction, sampling system, including sample introduction pipeline 1,
1 sample introduction end of the sample introduction pipeline is provided with three-way valve 2, and the another two port of the three-way valve 2 is respectively feed liquor port and air inlet
Port, also includes the first pump 3 and the second pump 4, is connected with input duct 5 and drain pipe road 6 on first pump 3, described go out liquid
Pipeline 6 and the connection of feed liquor port, second pump 4 include that admission line 7 and outlet pipe 8, the outlet pipe 8 are led to air inlet
Mouth connection, first pump 3 are duplex plunger pump,
During work, first the second pump 4 outwardly gas receiver pumping, three-way valve 2 are turned to, and air inlet port is connected, the second plunger valve
To water influent pipeline ventilation purging, then three-way valve 2 is turned to, and feed liquor port is connected, and two pump interval work of the first pump 3 are constantly to entering
Sample pipeline 1 carries out feed liquor operation, and the liquid in sample introduction pipeline 1 enters sample introduction object, after the first pump 3 completes quantitative liquid-filling, three
Port valve 2 is turned to, and air inlet port is connected, and the dead volume hydrops of residual is all pushed by the second plunger valve to water influent pipeline ventilation purging
Sample introduction object.
The sampling device and sample injection method of the application can realize single continuous sample introduction；Fixed Time Interval, quantitative sample injection；
Fixed Time Interval, non-quantitative sample introduction；Not Fixed Time Interval, quantitative sample injection；Not Fixed Time Interval, non-quantitative sample introduction；And
Dead volume can at utmost be reduced, increase sample introduction precision.
Particular embodiments described above, has been carried out to the purpose of the present invention, technical scheme and beneficial effect further in detail
Describe bright, the be should be understood that specific embodiment that the foregoing is only the present invention in detail, be not limited to the present invention, all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements that is done etc., should be included in the guarantor of the present invention
Within the scope of shield.
Claims (5)
1. a kind of reduce dead volume liquid sampling system, including sample introduction pipeline (1), it is characterised in that：Sample introduction pipeline (1)
Sample introduction end is provided with three-way valve (2), and the another two port of three-way valve (2) is respectively feed liquor port and air inlet port, also wraps
Include the first pump (3) and the second pump (4), on the first pump (3), be connected with input duct (5) and drain pipe road (6), described go out liquid
Pipeline (6) and the connection of feed liquor port, described second pump (4) include admission line (7) and outlet pipe (8), the outlet pipe
(8) it is connected with air inlet port.
2. as claimed in claim 1 a kind of reduce dead volume liquid sampling system, it is characterised in that：First pump (3)
For duplex plunger pump.
3. as claimed in claim 1 a kind of reduce dead volume liquid sampling system, it is characterised in that：First pump includes
Two single plunger pumps, the drain pipe road of described two single plunger pumps connect with feed liquor port.
4. one kind reduces dead volume Liquid sample introduction method, including：
A, three-way valve (2) is switched to the second pump (4), the second pump (4) blows air into sample introduction pipeline (1), to sample introduction pipeline (1)
Purged；
B, three-way valve (2) is switched to the first pump (3), sample introduction pipeline (1) connects sample introduction object, the first pump (3) is according to setting
Liquid needed for speed to sample introduction pipeline (1) injection；
C, three-way valve (2) is switched to the second pump (4), gas is passed through sample introduction pipeline (1) according to the speed for setting by the second pump (4)
In, the liquid in pipeline is pushed sample introduction object.
5. one kind as claimed in claim 4 reduces dead volume Liquid sample introduction method, it is characterised in that：First pump is twin columns
Plug pump or two drain pipe roads are and the single plunger pump that connects of feed liquor port；
In step B, required liquid is caused uninterruptedly to sample introduction pipeline according to setting by duplex plunger pump or two single plunger pumps
Speed injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611115962.7A CN106501539B (en) | 2016-12-07 | 2016-12-07 | A kind of sampling system and sample injection method reducing dead volume liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611115962.7A CN106501539B (en) | 2016-12-07 | 2016-12-07 | A kind of sampling system and sample injection method reducing dead volume liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106501539A true CN106501539A (en) | 2017-03-15 |
| CN106501539B CN106501539B (en) | 2019-05-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611115962.7A Active CN106501539B (en) | 2016-12-07 | 2016-12-07 | A kind of sampling system and sample injection method reducing dead volume liquid |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019179300A1 (en) * | 2018-03-21 | 2019-09-26 | 北京市隆福医院 | Dual-chamber suction filtering device and filtering method |
| CN110531100A (en) * | 2019-08-30 | 2019-12-03 | 中国航发北京航空材料研究院 | A kind of liquid feeding method |
| CN110658035A (en) * | 2019-08-30 | 2020-01-07 | 中国航发北京航空材料研究院 | Liquid adding method applied to injection pump |
| CN111830270A (en) * | 2020-07-09 | 2020-10-27 | 迪瑞医疗科技股份有限公司 | Precise sample adding system, in-vitro diagnosis equipment and precise sample adding method |
| CN112545692A (en) * | 2020-11-26 | 2021-03-26 | 深圳素士科技股份有限公司 | Flushing assembly, oral cavity flushing device and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019179300A1 (en) * | 2018-03-21 | 2019-09-26 | 北京市隆福医院 | Dual-chamber suction filtering device and filtering method |
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| CN110658035A (en) * | 2019-08-30 | 2020-01-07 | 中国航发北京航空材料研究院 | Liquid adding method applied to injection pump |
| CN111830270A (en) * | 2020-07-09 | 2020-10-27 | 迪瑞医疗科技股份有限公司 | Precise sample adding system, in-vitro diagnosis equipment and precise sample adding method |
| CN111830270B (en) * | 2020-07-09 | 2023-08-08 | 迪瑞医疗科技股份有限公司 | Precise sample adding system, in-vitro diagnosis equipment and precise sample adding method |
| CN112545692A (en) * | 2020-11-26 | 2021-03-26 | 深圳素士科技股份有限公司 | Flushing assembly, oral cavity flushing device and method |
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| Publication number | Publication date |
|---|---|
| CN106501539B (en) | 2019-05-21 |
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