CN106199032B - A kind of sample injection method of no cross contamination - Google Patents
A kind of sample injection method of no cross contamination Download PDFInfo
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- CN106199032B CN106199032B CN201610495365.5A CN201610495365A CN106199032B CN 106199032 B CN106199032 B CN 106199032B CN 201610495365 A CN201610495365 A CN 201610495365A CN 106199032 B CN106199032 B CN 106199032B
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- reagent
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- way valve
- syringe pump
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of sample injection method of no cross contamination, the sample injection method is equipped with syringe pump, multiple-way valve and soda liquor container, reagent container and waste fluid container：The syringe pump is equipped with first mouth of pipe and second mouth of pipe connected with syringe pump cavity volume, and first mouth of pipe is connected by the first pipeline equipped with control valve with soda liquor container, and second mouth of pipe is connected by the main interface of the second pipeline and multiple-way valve；The stub interface of the multiple-way valve includes an air inlet, a waste liquid outlet, a liquid outlet being connected with external container and some injection ports, the injection port reagent container connection.Sample injection method of the present invention separates multiple-way valve spool and the solution in reagent cartridge by gas, it may be implemented in during sample introduction, spool groove is avoided to be contacted with retaining reagent in each injection port, cause the situation of cross contamination, the effective accuracy for improving analysis of experiments, and the present invention is novel in design, it is easy to operate, it is easy to implement, easy to promote the use of.
Description
Technical field
The present invention relates to chemical field, is specially a kind of sample injection method of no cross contamination.
Background technology
Injector is a kind of chemical apparatuses, can be inserted sample to be tested in analyzing container by it, medical medicine analyze,
It is widely used in the multiple fields such as environment monitoring, inspection and quarantine.In the case of more than sample to be analysed or reagent type, sample introduction
Device usually with leading to more（Commutation）Valve is used cooperatively, but multiple-way valve, during inlet is switched, valve core rotation, connects main interface
Can have an opportunity to contact with the reagent solution retained in non-targeted branch's inlet with the groove of branch inlet, easily make each reagent
Cross contamination is produced during main interface is shared, have impact on the accuracy of analysis of experiments to a certain extent.
The content of the invention
The technical purpose of the present invention is to provide a kind of sample injection method of no cross contamination, existing in the prior art to solve
Problem.
To realize above-mentioned technical purpose, technical solution provided by the invention is：
A kind of sample injection method of no cross contamination, it is characterised in that equipped with syringe pump, multiple-way valve and soda liquor container, examination
Agent container and waste fluid container：
The syringe pump is equipped with first mouth of pipe and second mouth of pipe connected with syringe pump cavity volume, and first mouth of pipe is by setting
The first pipeline for having the first control valve is connected with soda liquor container, the master that second mouth of pipe passes through the second pipeline and multiple-way valve
Interface connects；
The multiple-way valve is provided around being distributed in multiple stub interfaces around main interface, and the multiple stub interface includes
One air inlet, a waste liquid outlet being connected with waste fluid container, a liquid outlet being connected with external container, remaining branch
Interface is injection port, is each connected one by one with corresponding reagent container；
The sample injection method comprises the following steps and the preprocessing process using following steps, sample introduction process：
（A）It is full of in the second pipeline under cleaning solution situation, multiple-way valve is switched into air inlet, operation injection pumping one
Step in quantitative gas to the second pipeline；
（B）Under the situation full of reagent in the reagent cartridge of connection injection port and corresponding reagent container, multiple-way valve is cut
The injection port is changed to, operation syringe pump is by the second pipeline in step（A）The gas release of middle suction is a certain amount of to arrive the reagent cartridge
In, the step of making by gas to separate between the reagent solution in the reagent cartridge and the injection port；
（C）Multiple-way valve switches to the injection port of setting, corresponding reagent is aspirated a certain amount of to described the by operation syringe pump
In two pipelines, make the step of connection injection port in the reagent cartridge of corresponding reagent container with being full of reagent, the second pipeline pilot scale
Agent passes through step with cleaning solution（A）The gas partition of middle suction；Multiple-way valve is switched into waste liquid outlet, operation syringe pump is by the
The step of gas in two pipelines is discharged into waste fluid container, makes to be full of cleaning solution in the second pipeline with reagent；
The preprocessing process includes：
First control valve is opened, multiple-way valve is switched into reset position, closes all passages of multiple-way valve, operation note
Penetrate pump and a certain amount of cleaning solution is aspirated out of soda liquor container, close the first control valve afterwards, multiple-way valve is switched into waste liquid
Outlet, operation syringe pump make cleaning solution enter in second pipeline, and leave one end of the second pipeline connection main interface
Certain gas or the gas in the second pipeline perform step after draining（A）；Circulation performs step in order afterwards（C）, step
（A）, step（B）, until one end of each bar reagent cartridge connection injection port is equipped with partition gas；
The sample introduction process includes：
（D）Multiple-way valve is switched into setting injection port, operation syringe pump by the reagent cartridge of the corresponding injection port every
The reagent of body and set amount of dying is pumped in second pipeline, multiple-way valve is switched to liquid outlet, operation syringe pump is outside
Injection meets the reagent of use demand in portion's container, and multiple-way valve is switched to waste liquid outlet, operates syringe pump by the second pipeline
In remaining gas or reagent from waste liquid outlet discharge the step of；Step is performed successively for the injection port afterwards（A）, step
Suddenly（B）.
On the basis of such scheme, further improve or preferable scheme further includes：
The air inlet is connected with the air inlet pipeline equipped with filter, prevents the impurity in gas from polluting reagent.
First control valve preferably uses two three-way electromagnetic valves.
During sample introduction, make the step（B）It is exterior that partition gas volume in middle push-in reagent pipeline is more than connection
The infusion pipeline volume of container and the liquid outlet, the step（D）In, will be a certain amount of when injecting reagent to external container
Partition gas is together pushed in external container with reagent, avoids reagent from being remained in infusion pipeline.
When the port of export of the infusion pipeline of connection liquid outlet and external container is arranged on the bottom of external container, the outside
Container is sealing container, is connected by being arranged on the vent line above external container with exterior space, on the vent line
Equipped with the second control valve, during the sample introduction, when injecting reagent to external container, second control valve is opened,
Make air pressure balance；After injection process, second control valve is momentarily turned off, prevents the reagent in the external container from returning
Drop down onto in woven hose.
Sample injection method of the present invention separates multiple-way valve spool with the solution in reagent cartridge, it can be achieved that in sample introduction mistake by gas
Cheng Zhong, avoids spool groove from being contacted with retaining reagent in each injection port, causes the situation of cross contamination, effective to improve experiment point
The accuracy of analysis, and the present invention is novel in design, it is easy to operate, it is easy to implement, easy to promote the use of.
Brief description of the drawings
Fig. 1 is the structure diagram of one embodiment of the invention；
Fig. 2 is the schematic diagram that gas separates cleaning solution and reagent solution in the second pipeline.
Embodiment
For the technical solution and design principle that the present invention is furture elucidated, below in conjunction with the accompanying drawings with specific embodiment to this
Invention is described further.
A kind of sample injection method of no cross contamination, holds equipped with syringe pump, multiple-way valve, soda liquor container, reagent container, waste liquid
Device, connecting line etc., as shown in Figure 1.
The syringe pump is double end syringe pump, equipped with liquid reservoir, piston, drive reciprocating motion of the pistons driving mechanism with
And first mouth of pipe and second mouth of pipe for liquid in-out, first mouth of pipe, second mouth of pipe are connected with syringe pump cavity volume, the first pipe
Mouth is connected by the first pipeline with soda liquor container, and first pipeline is equipped with the first control valve of control piper break-make,
It is preferred that use two three-way electromagnetic valves.The pure water as cleaning solution is loaded with the soda liquor container.Second mouth of pipe passes through
The main interface of two pipelines and multiple-way valve connects, and in the present embodiment, the multiple-way valve uses eight ways valve, and the driving mechanism is advanced
When, syringe pump is pushed, and when driving mechanism retracts, syringe pump is aspirated.
The multiple-way valve is equipped with main interface and uniform ring around eight stub interfaces being distributed in around main interface, eight branches
Interface include an air inlet, a waste liquid outlet being connected with waste fluid container, one be connected with external container go out liquid
Mouthful, remaining stub interface is injection port, each reagent container corresponding with one（Reagent 1- reagents 5）Connection.The waste liquid
Outlet, air inlet are located at the left and right sides of liquid outlet respectively.The air inlet pipeline of connection air inlet is equipped with air filter, can
Avoid the contaminants reagent in air.
In the present embodiment, first pipeline, the second pipeline, connection container and the branch mouth of pipe pipeline, be connected main interface
Internal diameter with the infusion pipeline of external container is between 1mm-2.5mm.
The sample injection method of the no cross contamination, including preprocessing process and sample introduction process：
（One）The preprocessing process is as follows：
First, first control valve is opened, multiple-way valve is switched into reset position（Between the Ji Liangge branches mouth of pipe
Position）, all passages of multiple-way valve are closed, according to flow scheme design, operation syringe pump aspirates enough pure water out of soda liquor container
Cleaning solution, closes the first control valve afterwards, multiple-way valve is switched to waste liquid outlet, operation syringe pump makes clear in its cavity volume
Washing lotion enters in second pipeline, retains a certain amount of gas or in the second pipeline in one end of the second pipeline connection main interface
Gas drain after（There is cleaning solution to enter in the pipeline of waste liquid outlet connection waste fluid container）Perform step（A）, the step
Suddenly（A）To be full of in the second pipeline under cleaning solution situation, multiple-way valve is switched into air inlet, operation injection pumping is a certain amount of
Step in gas to the second pipeline.
Secondly, step is performed（C）, multiple-way valve is switched into a certain injection port, exemplified by corresponding to the injection port of reagent 1, behaviour
Make syringe pump to aspirate reagent 1 in second pipeline, make the reagent cartridge of the connection injection port and 1 container of reagent
In be full of reagent 1（Gas is discharged）, reagent 1 passes through step with cleaning solution in the second pipeline（A）The gas partition of middle suction, such as
Shown in Fig. 2.Multiple-way valve is switched into waste liquid outlet afterwards, operates syringe pump by the partition gas in the second pipeline and reagent 1
It is discharged into waste fluid container, the second pipeline is again filled with cleaning solution.
And then perform step（A）；
After again, step is performed for 1 injection port of reagent（B）, the step（B）To hold in connection injection port and corresponding reagent
In the reagent cartridge of device under the situation full of reagent, multiple-way valve is switched to the injection port, operation syringe pump exists the second pipeline
Step（A）The gas release of middle suction is a certain amount of into the reagent cartridge, makes reagent solution and the sample introduction in the reagent cartridge
The step of being separated between mouthful by gas.
Next, arriving reagent 5 for reagent 2, execution step is circulated using same operating method in order（C）, step
（A）, step（B）, until one end that five reagent cartridges connect injection port is respectively formed partition gas.It is more logical then during formal sample introduction
Valve core connects main interface when being aligned with the groove of stub interface with injection port in rotation process is switched, on spool, groove and
Reagent solution in reagent cartridge does not contact, and can avoid cross contamination.
（Two）The sample introduction process includes：
Step（D）, multiple-way valve is switched into setting injection port, exemplified by injecting reagent 1 to external container, operates syringe pump
Partition gas in the reagent cartridge of corresponding 1 injection port of reagent is pumped to second pipeline with the reagent 1 more than demand
In.Multiple-way valve is switched into liquid outlet, operation syringe pump injects the reagent for meeting use demand into external container, then will lead to more
Vavle switching to waste liquid outlet, operation syringe pump discharges the remaining gas or reagent in the second pipeline from waste liquid outlet.
Afterwards, step is performed successively for 1 injection port of reagent（A）, step（B）, make reagent in 1 reagent cartridge of reagent with
Multiple-way valve spool separates yet by gas.
Same operating method, can be used in the sample introduction process that reagent 2 arrives reagent 5 by the requirement of root experimental analysis afterwards
In.
In step（D）In, when aspirating reagent into the second pipeline, injecting reagent calculating demand to external container, need to calculate
Enter the error that inevitable lower tape comes in multiple-way valve groove and pipeline.Or in step（D）In, according to 1 demand of reagent,
The reagent 1 of equivalent demand is pumped in the second pipeline by operation syringe pump, when injecting reagent to external container, by the second pipeline
In partition gas and reagent 1 be together pushed in external container, reagent 1 is squeezed out into infusion pipeline using gas is separated, is kept away
It is excused from an examination the agent error that lower tape comes in infusion pipeline, the step at this time（B）Partition gas body in middle push-in reagent pipeline
Product need to be more than the woven hose volume of connection external container and the liquid outlet, make reagent completely into after in external container, and second
Cleaning solution in pipeline need not enter in infusion pipeline.
In above-described embodiment, if the port of export of the woven hose of connection liquid outlet and external container is arranged on the bottom of external container
Portion.The external container need to be set to sealing container, a blow vent connected with outside is set in the top of external container, it is described
Vent line is equipped with the second control valve（It is not shown）.During above-mentioned sample introduction, when injecting reagent to external container, open
Second control valve, makes the air pressure balance in external container, and after injection process, second control need to be momentarily turned off
Valve processed, avoids reagent from flowing back.
In the present patent application file, " a certain amount of " described in many places simultaneously refers not to identical parameter, also refers not to specifically join
Number, it will be appreciated that to be set according to demand, and have no effect on skilled artisan understands that this programme.
Basic principle, main feature and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, the present invention
Claimed scope is delineated by the appended claims, the specification and equivalents thereof from the appended claims.
Claims (5)
1. a kind of sample injection method of no cross contamination, it is characterised in that equipped with syringe pump, multiple-way valve and soda liquor container, reagent
Container and waste fluid container：
The syringe pump is equipped with first mouth of pipe and second mouth of pipe that are connected with syringe pump cavity volume, and first mouth of pipe passes through equipped with the
First pipeline of one control valve is connected with soda liquor container, and second mouth of pipe passes through the second pipeline and the main interface of multiple-way valve
Connection；
The multiple-way valve is provided around being distributed in multiple stub interfaces around main interface, and the multiple stub interface includes one
Air inlet, a waste liquid outlet being connected with waste fluid container, a liquid outlet being connected with external container, remaining stub interface
It is injection port, is each connected one by one with corresponding reagent container；
The sample injection method comprises the following steps and the preprocessing process using following steps, sample introduction process：
（A）It is full of in the second pipeline under cleaning solution situation, multiple-way valve is switched into air inlet, operation injection pumping is a certain amount of
Step in gas to the second pipeline；
（B）Under the situation full of reagent in the reagent cartridge of connection injection port and corresponding reagent container, multiple-way valve is switched to
The injection port, operation syringe pump is by the second pipeline in step（A）The gas release of middle suction is a certain amount of into the reagent cartridge, makes
The step of being separated between reagent solution and the injection port in the reagent cartridge by gas；
（C）Multiple-way valve is switched to the injection port of setting, corresponding reagent is aspirated second pipe by operation syringe pump
Lu Zhong, the step of making be full of reagent in the reagent cartridge of the connection injection port and corresponding reagent container, in the second pipeline reagent and
Cleaning solution passes through step（A）The gas partition of middle suction；Multiple-way valve is switched into waste liquid outlet, operation syringe pump is managed second
The step of gas in road is discharged into waste fluid container, makes to be full of cleaning solution in the second pipeline with reagent；
The preprocessing process includes：
First control valve is opened, multiple-way valve is switched into reset position, closes all passages of multiple-way valve, operates syringe pump
A certain amount of cleaning solution is aspirated out of soda liquor container, closes the first control valve afterwards, multiple-way valve is switched into waste liquid discharge
Mouthful, operation syringe pump makes cleaning solution enter in second pipeline, and one end of the second pipeline connection main interface is left necessarily
Gas or the gas in the second pipeline perform step after draining（A）；Circulation performs step in order afterwards（C）, step（A）、
Step（B）, until one end of each bar reagent cartridge connection injection port is equipped with partition gas；
The sample introduction process includes：
（D）Multiple-way valve is switched into setting injection port, operates syringe pump by the partition gas in the reagent cartridge of the corresponding injection port
The reagent of body and set amount is pumped in second pipeline, multiple-way valve is switched to liquid outlet, operation syringe pump holds to outside
Injection meets the reagent of use demand in device, multiple-way valve is switched to waste liquid outlet, operation syringe pump is by the second pipeline
Remaining gas or reagent from waste liquid outlet discharge the step of；Step is performed successively for the injection port afterwards（A）, step
（B）.
2. the sample injection method of a kind of no cross contamination according to claim 1, it is characterised in that the air inlet is with being equipped with
The air inlet pipeline connection of filter.
A kind of 3. sample injection method of no cross contamination according to claim 1, it is characterised in that first control valve
For two three-way electromagnetic valves.
4. the sample injection method of a kind of no cross contamination according to claim 1, it is characterised in that during sample introduction, make
The step（B）Partition gas volume in middle push-in reagent pipeline is more than the woven hose of connection external container and the liquid outlet
The appearance of a street is accumulated, the step（D）In, when injecting reagent to external container, a certain amount of partition gas is together pushed to reagent
In external container.
5. the sample injection method of a kind of no cross contamination according to claim 1, it is characterised in that connection liquid outlet and outside
The port of export of the infusion pipeline of container is arranged on the bottom of external container；The external container is sealing container, by being arranged on
Vent line above external container is connected with exterior space, and the vent line is equipped with the second control valve, it is described into
During sample, when injecting reagent to external container, second control valve is opened, after injection process, be momentarily turned off institute
State the second control valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610495365.5A CN106199032B (en) | 2016-06-30 | 2016-06-30 | A kind of sample injection method of no cross contamination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610495365.5A CN106199032B (en) | 2016-06-30 | 2016-06-30 | A kind of sample injection method of no cross contamination |
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| CN106199032A CN106199032A (en) | 2016-12-07 |
| CN106199032B true CN106199032B (en) | 2018-05-08 |
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Denomination of invention: Cross pollution-free sample introduction method Effective date of registration: 20191119 Granted publication date: 20180508 Pledgee: Nanjing Bank Co., Ltd. Chengnan Branch Pledgor: NANJING RUNZE FLUID CONTROLEQUIPMENT CO., LTD. Registration number: Y2019320000291 |
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