CN214211537U - Immunoblotting test system gel glass board belt cleaning device - Google Patents
Immunoblotting test system gel glass board belt cleaning device Download PDFInfo
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- CN214211537U CN214211537U CN202023257904.2U CN202023257904U CN214211537U CN 214211537 U CN214211537 U CN 214211537U CN 202023257904 U CN202023257904 U CN 202023257904U CN 214211537 U CN214211537 U CN 214211537U
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Abstract
The device for cleaning the rubber-made glass plate for the immunoblotting test comprises an ultrasonic cleaning machine and a hot air blower, wherein a cover body is arranged at the top of the ultrasonic cleaning machine, one side of the cover body is connected with the ultrasonic cleaning machine in a hinged mode, the other side of the cover body is connected with the ultrasonic cleaning machine through a lock catch, and a cavity is formed in the cover body; the bottom surface of the cover body is provided with a plurality of air outlet holes, and an air outlet of the hot air blower is connected with the cover body through a hot air pipe; a plurality of partition pieces are arranged in the ultrasonic cleaning machine side by side, each partition piece consists of two partition plates, one partition plate is fixed on the upper part of a front wall plate of the ultrasonic cleaning machine, the other partition plate is fixed on the upper part of a rear wall plate of the ultrasonic cleaning machine, and the two partition plates are arranged in a front-back corresponding mode; a slot is formed between two adjacent separating pieces, and a glass plate clamping piece is inserted into each slot; the ultrasonic cleaning machine is provided with a water inlet joint and a water outlet joint. To sum up, the utility model is suitable for a vast scientific research worker relates to the face extensively, can realize automatic washing stoving, and a large amount of samples are washd in the concurrent operation.
Description
Technical Field
The utility model belongs to the technical field of the medical treatment, concretely relates to experimental system of immunoblotting glass board belt cleaning device.
Background
Western Blot, Western immunoblotting (immunoblot assay), is a commonly used assay in molecular biology, biochemistry and immunogenetics. The basic principle is to stain the cell or biological tissue sample treated by gel electrophoresis through specific antibody and obtain the information of the expression condition of specific protein in the analyzed cell or tissue by analyzing the stained position and the stained depth. Western Blot detects the expression level of protein in tissues or cells, and is widely applied to the aspects of gene expression research on protein level, antibody activity detection, early disease diagnosis and the like. Western Blot was performed by polyacrylamide gel electrophoresis, and the test substance was a protein, "probe" was an antibody, "and" secondary antibody for color development "was labeled. SDS-PAGE allows protein samples to be separated and transferred to a solid support, such as nitrocellulose membrane (NC). The solid phase carrier can adsorb protein and keep the type of polypeptide separated by electrophoresis and the biological activity of the polypeptide unchanged. The transferred NC membrane is called a blot (blot) and treated with a protein solution (e.g. 5% BSA or skim milk powder solution) to block the hydrophobic binding sites on the NC membrane. Taking protein or polypeptide on a solid phase carrier as an antigen, carrying out immunoreaction with a corresponding antibody, then carrying out reaction with a second antibody labeled by enzyme or isotope, and carrying out substrate chromogenic or autoradiography to detect the protein component expressed by the specific target gene separated by electrophoresis.
With the development of experimental technology, Western blot is more and more widely applied, and most scientific researches and molecular experiments need the verification and detection of Western blot. The experiment technology is complex in process and long in time consumption, and comprises the steps of protein extraction, protein quantification, gel preparation, electrophoresis, membrane conversion, blocking, antibody incubation, development and the like, so that the condition of shortening the early preparation time is a condition which needs to be improved urgently. The preparation of the gel is a key step of success or failure of operation, the problems of nonuniform gel, impurity content and the like directly influence the finished strip product, and the guarantee of the cleanness degree of the glue-making glass plate is a basic problem which must be solved by all researchers. The current cleaning method for the glue-making glass plate is mostly manual, and the basic operation steps are that the glass plate is brushed by using cleaning agents such as detergent or washing powder and the like, then the glass plate is washed by ddH2O, finally, the side of the glass plate, which is in contact with the glue, is placed on a plate frame in an inclined downward mode, and the glass plate is naturally aired or placed in a drying box for 1 hour. The process is long in time consumption, manpower and material resources which are not in direct proportion to the obtained value are consumed, the glass plate is easily cleaned by manual brushing, and some fine depressions can coagulate glue particles which cannot be distinguished by naked eyes, so that uneven glue blocks can be caused; on the other hand, if the scrubbing tool material is hard or the scrubbing force is too big, the glass plate will produce the mar and can't reuse, has increased the consumptive material volume.
SUMMERY OF THE UTILITY MODEL
The utility model discloses a solve the weak point among the prior art, provide a cleaning efficiency height, sanitization, can carry out the experimental system of air-dried immunoblotting glass board belt cleaning device to the glass board.
In order to solve the technical problem, the utility model adopts the following technical scheme: the device for cleaning the rubber-making glass plate for the immunoblotting test comprises an ultrasonic cleaning machine and an air heater, wherein a cover body is arranged at the top of the ultrasonic cleaning machine, one side of the cover body is connected with the ultrasonic cleaning machine in a hinged mode, the other side of the cover body is connected with the ultrasonic cleaning machine through a lock catch, a cavity is formed in the cover body, a plurality of air outlet holes are formed in the bottom surface of the cover body, and an air outlet of the air heater is connected with the cover body through an air heating pipe;
a plurality of partition pieces are arranged in the ultrasonic cleaning machine side by side, each partition piece consists of two partition plates, one partition plate is fixed on the upper part of a front wall plate of the ultrasonic cleaning machine, the other partition plate is fixed on the upper part of a rear wall plate of the ultrasonic cleaning machine, and the two partition plates are arranged in a front-back corresponding mode; a slot is formed between two adjacent separating pieces, and a glass plate clamping piece is inserted into each slot; the ultrasonic cleaning machine is provided with a water inlet joint and a water outlet joint.
The glass board holder includes the mounting panel of rectangular shape, the top of mounting panel is provided with the handle, the both ends support at ultrasonic cleaner's preceding wallboard and back wallboard top of mounting panel, the bottom surface rear side of mounting panel is fixed with fixed grip block, the bottom surface front side of mounting panel is provided with movable grip block, the slot hole has been seted up to the front portion of mounting panel, the upper end of activity grip block is fixed with the bolt, the bolt upwards passes the slot hole perpendicularly, the upper portion threaded connection of bolt has set nut, the top surface roof pressure contact of set nut bottom surface and mounting panel.
The opposite sides of the movable clamping plate and the fixed clamping plate are provided with rubber cushions.
Adopt above-mentioned technical scheme, the utility model discloses following beneficial effect has: the utility model is suitable for the vast researchers, has wide relating range, can realize automatic washing and drying, and simultaneously operates and cleans a large number of samples; compared with the traditional manual cleaning, the device realizes automatic ultrasonic cleaning and clean hot air drying, greatly improves the experimental efficiency, reduces the manual error, and is a high-quality achievement combining scientific research and modern technology.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic view of the structure of the glass sheet holder.
Detailed Description
As shown in fig. 1 and 2, the device for cleaning a rubber-made glass plate for an immunoblotting test of the present invention comprises an ultrasonic cleaning machine 1 and an air heater 5, wherein a cover body 2 is provided at the top of the ultrasonic cleaning machine 1, one side of the cover body 2 is connected with the ultrasonic cleaning machine 1 in a hinged manner, the other side of the cover body 2 is connected with the ultrasonic cleaning machine 1 through a latch 3, a cavity is provided inside the cover body 2, a plurality of air outlets 4 are provided on the bottom surface of the cover body 2, and an air outlet of the air heater 5 is connected with the cover body 2 through a hot air pipe 6;
a plurality of partition pieces are arranged in the ultrasonic cleaning machine 1 side by side, each partition piece is composed of two partition plates 7, one partition plate 7 is fixed on the upper portion of a front wall plate 8 of the ultrasonic cleaning machine 1, the other partition plate 7 is fixed on the upper portion of a rear wall plate 9 of the ultrasonic cleaning machine 1, and the two partition plates 7 are arranged in a front-rear corresponding mode; a slot is formed between two adjacent separating pieces, and a glass plate clamping piece is inserted into each slot; the ultrasonic cleaning machine 1 is provided with a water inlet joint 10 and a water outlet joint 11.
The glass plate clamping piece comprises a long-strip-shaped mounting plate 12, a handle 13 is arranged at the top of the mounting plate 12, two end parts of the mounting plate 12 are supported at the top of a front wall plate 8 and a rear wall plate 9 of the ultrasonic cleaning machine 1, a fixed clamping plate 14 is fixed at the rear side of the bottom surface of the mounting plate 12, a movable clamping plate 15 is arranged at the front side of the bottom surface of the mounting plate 12, a long hole 16 is formed in the front part of the mounting plate 12, a bolt 17 is fixed at the upper end of the movable clamping plate 15, the bolt 17 vertically upwards penetrates through the long hole 16, a positioning nut 18 is in threaded connection with the upper part of the bolt 17, and the bottom surface of the positioning nut 18 is in top pressure contact with the top surface of the mounting plate 12.
The opposite sides of the movable clamping plate 15 and the fixed clamping plate 14 are provided with rubber cushions 19.
The utility model discloses when using, clip the glass board with the glass board holder earlier, the concrete operation is, contradict one side of glass board on fixed clamping plate 14, then remove movable clamping plate 15, carry out the centre gripping to the glass board another side, then revolve wrong set nut 18, make set nut 18 and the top surface roof pressure contact of mounting panel 12, accomplish the fixing to bolt 17 through set nut 18, restriction bolt 17 removes in slot hole 16, the final completion is fixed to movable clamping plate 15, realize the centre gripping to the glass board through fixed clamping plate 14 and movable clamping plate 15, the setting of rubber cushion 19 is used for protecting the glass board, prevent that it from receiving the damage; secondly, the position of the movable clamping plate 15 is changed by adjusting the position of the bolt 17 in the long hole 16, so as to clamp glass plates with different sizes;
then lifting the lifting handle 13, inserting the glass plate on the glass plate clamping piece into the slot, supporting two end parts of the mounting plate 12 at the tops of the front wall plate 8 and the rear wall plate 9 of the ultrasonic cleaning machine 1, and limiting the glass plate by arranging the partition piece to prevent the glass plate from shaking back and forth in the ultrasonic cleaning process; then pour into water liquid into in to ultrasonic cleaner 1 through water supply connector 10, and guarantee that the liquid level is higher than the top of glass board, then utilize ultrasonic cleaning, realize the circulation water injection that drains through water supply connector 10 and water connectors 11, wash many times the glass board, after finishing washing, start air heater 5, air heater 5 sends into the inner chamber of lid 2 with hot-blast through hot-blast main 6, have exhaust vent 4 to blow to ultrasonic cleaner 1's inner chamber again, hot-blast air is air-dried glass board surface water liquid.
The present embodiment is not intended to limit the shape, material, structure, etc. of the present invention in any form, and all of the technical matters of the present invention belong to the protection scope of the present invention to any simple modification, equivalent change and modification made by the above embodiments.
Claims (3)
1. Experimental system of immunoblotting glass board belt cleaning device which characterized in that: the ultrasonic cleaning machine comprises an ultrasonic cleaning machine and an air heater, wherein a cover body is arranged at the top of the ultrasonic cleaning machine, one side of the cover body is connected with the ultrasonic cleaning machine in a hinged mode, the other side of the cover body is connected with the ultrasonic cleaning machine through a lock catch, a cavity is formed in the cover body, a plurality of air outlet holes are formed in the bottom surface of the cover body, and an air outlet of the air heater is connected with the cover body through a hot air pipe;
a plurality of partition pieces are arranged in the ultrasonic cleaning machine side by side, each partition piece consists of two partition plates, one partition plate is fixed on the upper part of a front wall plate of the ultrasonic cleaning machine, the other partition plate is fixed on the upper part of a rear wall plate of the ultrasonic cleaning machine, and the two partition plates are arranged in a front-back corresponding mode; a slot is formed between two adjacent separating pieces, and a glass plate clamping piece is inserted into each slot; the ultrasonic cleaning machine is provided with a water inlet joint and a water outlet joint.
2. The immunoblotting test gel-making glass plate washing apparatus according to claim 1, wherein: the glass board holder includes the mounting panel of rectangular shape, the top of mounting panel is provided with the handle, the both ends support at ultrasonic cleaner's preceding wallboard and back wallboard top of mounting panel, the bottom surface rear side of mounting panel is fixed with fixed grip block, the bottom surface front side of mounting panel is provided with movable grip block, the slot hole has been seted up to the front portion of mounting panel, the upper end of activity grip block is fixed with the bolt, the bolt upwards passes the slot hole perpendicularly, the upper portion threaded connection of bolt has set nut, the top surface roof pressure contact of set nut bottom surface and mounting panel.
3. The immunoblotting test gel-making glass plate washing apparatus according to claim 2, wherein: the opposite sides of the movable clamping plate and the fixed clamping plate are provided with rubber cushions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023257904.2U CN214211537U (en) | 2020-12-30 | 2020-12-30 | Immunoblotting test system gel glass board belt cleaning device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023257904.2U CN214211537U (en) | 2020-12-30 | 2020-12-30 | Immunoblotting test system gel glass board belt cleaning device |
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| Publication Number | Publication Date |
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| CN214211537U true CN214211537U (en) | 2021-09-17 |
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| CN202023257904.2U Active CN214211537U (en) | 2020-12-30 | 2020-12-30 | Immunoblotting test system gel glass board belt cleaning device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114871197A (en) * | 2022-04-07 | 2022-08-09 | 安徽高芯众科半导体有限公司 | Ultrasonic oscillation cleaning machine for semiconductor precision parts |
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2020
- 2020-12-30 CN CN202023257904.2U patent/CN214211537U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114871197A (en) * | 2022-04-07 | 2022-08-09 | 安徽高芯众科半导体有限公司 | Ultrasonic oscillation cleaning machine for semiconductor precision parts |
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