CN114323833A - Immunohistochemical specimen processing device and method adopting paraffin section mode - Google Patents
Immunohistochemical specimen processing device and method adopting paraffin section mode Download PDFInfo
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Abstract
The invention discloses an immunohistochemical specimen processing device and a processing method adopting a paraffin section mode, wherein the processing device comprises a rack, an incubation bin module, a feeding mechanism, a sample-adding scanning component and a control unit, the incubation bin module comprises a plurality of incubation bins, the incubation bins are divided into a plurality of specimen processing areas, each specimen processing area comprises at least one incubation bin, each incubation bin comprises an incubation seat, the incubation seat is provided with a liquid storage tank, a heater and a temperature sensor, a slide glass module with a slide glass is placed in the liquid storage tank, and the control unit is connected with the heater and the temperature sensor of each incubation bin and independently controls each specimen processing area to process the specimen in a subarea manner; the incubation cabin module has a plurality of sample processing areas sharing a feeding mechanism and a sample adding scanning component. It has the following advantages: each zone operates independently without interference, can process in a partitioned and batched manner, and can complete the test at different time, in different environments and under different test conditions.
Description
Technical Field
The invention relates to the technical field of diagnostic medical instruments, in particular to an immunohistochemical specimen processing device and method adopting a paraffin section mode.
Background
The immunohistochemical specimen treatment device adopting the paraffin section mode comprises a rack, an incubator, a feeding mechanism, a sample-adding scanning component and a control unit, wherein the incubator and the feeding mechanism are arranged on the rack, the sample-adding scanning component is connected with the feeding mechanism, the incubator comprises a liquid storage tank for liquid storage medium, a heater for heating the liquid storage medium and a temperature sensor for detecting the temperature of the liquid storage medium, a slide glass module with a slide glass is arranged in the liquid storage tank, and the slide glass module is heated through the liquid storage medium. Because all slide glass modules are placed in an incubator and in a processing flow (heating time, temperature, all working procedures and the like are shared), the following defects exist:
(1) the immunohistochemical specimen treatment is a complete flow, the flow cannot be interrupted after the start, otherwise, the final result is influenced, once the immunohistochemical specimen treatment instrument on the current market starts to execute a specified flow, the second specimen treatment can be carried out only after the flow is finished, one of the two is that the duration of the flow is long (more than 3 hours), a large amount of time is wasted, and the detection efficiency of pathological sections in hospital departments is seriously influenced; secondly, the immunohistochemical specimen processing only supports one-time specimen processing, the specimen cannot be loaded midway, and if the specimen is forcibly suspended and added, the test result is influenced, so that the test result is wrong;
(2) all samples can only be processed in the same environment (heating time, temperature, all working procedures and the like are shared), different environments for different samples cannot be met, and one of the environments needs more extra equipment or instruments to ensure the integrity of the flow of each sample; secondly, when immunohistochemical testing is carried out, the required repairing temperatures of different pathological tissues are slightly different, and when different pathological tissues are treated by one repairing temperature and are treated by one same testing program, the final effect of the immunohistochemical testing can be influenced.
Disclosure of Invention
The invention provides an immunohistochemical specimen processing device and a immunohistochemical specimen processing method adopting a paraffin section mode, which overcome the defects of an immunohistochemical specimen processing device adopting a paraffin section mode in the background technology.
One of the technical schemes adopted by the invention for solving the technical problems is as follows: the immunohistochemical specimen processing device adopting the paraffin section mode comprises a rack, an incubation bin module, a feeding mechanism, a sample adding scanning component and a control unit, wherein the incubation bin module and the feeding mechanism are arranged on the rack, and the sample adding scanning component is connected with the feeding mechanism; the incubation bin module comprises a plurality of specimen processing areas, each specimen processing area comprises at least one incubation bin, each incubation bin comprises an incubation seat, each incubation seat is provided with a liquid storage tank for storing liquid medium, a heater for heating the liquid medium and a temperature sensor for detecting the temperature of the liquid medium, a slide glass module with a slide glass is placed in the liquid storage tank, and the control unit is connected with the heater and the temperature sensor of each incubation bin and independently controls each specimen processing area to process the specimen in a partitioned mode; the incubation bin module has a plurality of sample processing areas sharing a feeding mechanism and a sample adding scanning component, and the control unit is connected with the feeding mechanism and the sample adding scanning component and conveys the sample adding scanning component to the required position of each incubation bin through the feeding mechanism.
In one embodiment: this hatch a plurality of storehouses of hatching of storehouse module and arrange and hatch storehouse side by side and can pull connection frame.
In one embodiment: the controller unit includes a plurality of control pieces that correspond to the plurality of sample processing areas one-to-one and control the heaters and the temperature sensors of the respective sample processing areas, respectively.
In one embodiment: each sample processing region includes an incubation compartment.
In one embodiment: the sample-loading scanning component comprises a sample-loading needle unit and a scanner mechanism, the scanner mechanism comprises a scanner which can at least identify the identification information of a slide glass arranged on the slide glass module, and the control unit controls the corresponding sample processing area according to the information obtained by scanning the identification information by the scanner.
In one embodiment: the slide module comprises a slide bracket, a slide assembly is placed on the slide bracket, and the slide assembly comprises a slide and a slide cover plate; the slide glass bracket is placed in the liquid storage tank, two ends of the slide glass bracket are supported on the incubation seat, the slide glass bracket is provided with a plurality of obliquely arranged insertion grooves, and the slide glass assembly is inserted along the slide glass bracket insertion grooves.
In one embodiment: the heater is installed on the bottom surface of the liquid storage tank, the temperature sensor is installed in the liquid storage tank, and the control unit adjusts the heating power output of the incubation bin heater according to the real-time detection temperature of the incubation bin temperature sensor.
In one embodiment: the device comprises a control unit, a sample loading scanning component, a sample loading groove, a sample loading micro-pump, a sample loading scanning component, a sample loading groove and a sample unloading groove, wherein the sample loading micro-pump is connected with the sample loading needle unit of the sample loading scanning component through a pipeline, the control unit controls a feeding mechanism and conveys the sample loading needle unit to the sample loading groove top of the slide glass component of each incubation bin slide glass module, and the control unit is connected with the sample loading micro-pump and controls the volume and the frequency of sampling and lofting through the control unit.
In one embodiment: the frame is provided with a reference platform, the incubation bin module is arranged on the reference platform, and the feeding mechanism takes the reference platform as a reference.
The second technical scheme adopted by the invention for solving the technical problems is as follows: the immunohistochemical specimen processing method adopts a paraffin section mode, the immunohistochemical specimen processing device comprises an incubation bin module, a sample adding scanning component and a control unit, the incubation bin module comprises a plurality of incubation bins, the incubation bins are divided into a plurality of specimen processing areas, each specimen processing area comprises at least one incubation bin, and the control unit is connected with each incubation bin and independently controls each specimen processing area to process specimens in a partition mode; the control unit stores a plurality of treatment protocols which are respectively suitable for different pathological tissue sections, the treatment protocols comprise heating temperature and heating time, and the control unit is connected with the sample-adding scanning component;
the processing method comprises the following steps:
and 3, processing the sample processing area by the control unit according to the called processing rule until the processing is finished, and executing the step 1 in the execution process of the step 3.
In one embodiment: the immunohistochemical sample processing device comprises a feeding mechanism, wherein the sample adding scanning component is connected with the feeding mechanism, a plurality of sample processing areas of the incubation cabin module share the feeding mechanism and the sample adding scanning component, and the control unit is connected with the feeding mechanism and the sample adding scanning component and conveys the sample adding scanning component to the required position of each incubation cabin through the feeding mechanism;
and (3) in the process of executing the steps 2 and 3, if a sample-adding scanning component is to be applied to a certain sample processing area according to the processing protocol, judging whether the application of the sample-adding scanning component is in a standby state, if so, applying, otherwise, waiting until the application is in the standby state, and marking the application as the standby state after the application is finished.
In one embodiment: setting a priority procedure for the treatment procedure; in the process of executing steps 2 and 3, if a sample-loading scanning component is applied to a certain sample processing area according to a processing protocol, executing the following steps: step A, judging whether the application of the sample-adding scanning component is in a standby state, if so, applying, otherwise, executing step B; step B, judging whether the sample processing area applies the sample adding scanning component to be a priority process, if so, executing step C1, otherwise, executing step C2; step C1, the sample processing area applies the sample-adding scanning component according to the processing rule, and continues to execute the original application after the use; step C2, wait until in standby state and mark as standby state after application is completed.
Compared with the background technology, the technical scheme has the following advantages:
divide a plurality of sample processing districts, every sample processing district includes at least one and incubates the storehouse, incubates the storehouse and establishes heater and temperature sensor, and the control unit connects each heater and the temperature sensor of incubating the storehouse and each sample processing district of independent control and handles the sample with the subregion, consequently overcomes the not enough and production following technological effect that the background art exists: (1) each zone operates independently without interference, can select rules according to needs, controls temperature independently, can process in a subarea and batch mode, and can complete tests in different time, different environments and different test conditions; (2) when one area starts to work, even if the area is not finished, the specimen can be loaded on other areas and the regulation can be operated, so that the purpose of loading a large amount of specimens for the second time (for example, more than 32 specimens) is achieved; (3) each area is independently controlled by temperature, reliable environmental factors can be provided for experiments, and test results are improved.
The plurality of control pieces correspond to the plurality of sample processing areas one by one, and the plurality of control pieces respectively control the heaters and the temperature sensors of the sample processing areas, so that the processing speed is improved.
The control unit selects a processing rule according to the information obtained by scanning the identification information by the scanner, controls the corresponding specimen processing area according to the processing rule, and improves the test result.
Drawings
The invention is further described with reference to the following figures and detailed description.
Fig. 1 is one of schematic perspective views of a treatment apparatus according to an embodiment.
Fig. 2 is a second perspective view of the processing apparatus according to the embodiment.
Fig. 3 is one of the schematic three-dimensional structures of the incubation chamber of the processing device according to the embodiment.
FIG. 4 is a schematic perspective view of an incubation seat of the treatment apparatus according to the embodiment.
Fig. 5 is a second schematic perspective view of the incubation chamber of the processing apparatus according to the embodiment.
Fig. 6 is a schematic perspective view of a slide module of the processing apparatus according to an embodiment.
Fig. 7 is a schematic diagram of a control flow of an immunohistochemical device.
Detailed Description
Referring to fig. 1 to 7, an immunohistochemical specimen processing apparatus using a paraffin section method includes a rack, an incubation chamber module, a feeding mechanism, a sample-adding scanning unit and a control unit, wherein the incubation chamber module and the feeding mechanism are both mounted on the rack, the sample-adding scanning unit is connected to the feeding mechanism, the incubation chamber module includes a plurality of specimen processing regions, each specimen processing region corresponds to an incubation chamber, the incubation chambers have the same structure and include an incubation seat 502, the incubation seat 502 is provided with a liquid storage tank 507 for storing a liquid medium, a heater for heating the liquid medium and a temperature sensor 503 for detecting the temperature of the liquid medium, and a slide module with a slide 509 is placed in the liquid storage tank 507. The control unit is connected with the heaters and the temperature sensors of the incubation bins and independently controls the specimen processing areas to process specimens in a partitioning mode, each area can select processing procedures according to pathological slide specimens, and processing steps of different processing procedures or/and temperature control temperature or/and heating time and the like are different so as to ensure that each specimen is processed according to required procedures to obtain an optimal test result, (1) partitioning cycle management is adopted, the normal use requirement of immunohistochemistry can be met, the specimens can be processed in a partitioning mode and in batches, and the immunohistochemistry specimen processing efficiency is effectively improved; (2) the slide glass specimens which need different operation flows can be processed in different environment areas, and the test result is improved; (3) after operation of a certain region is finished, secondary specimen loading can be carried out in the region, and the treatment capacity of the immunohistochemical specimen is greatly improved.
In the specific embodiment, four incubation bins are taken as an example for explanation, the four incubation bins are respectively an incubation bin a 5, an incubation bin B4, an incubation bin C3 and an incubation bin D2, and the four incubation bins 2, 3, 4 and 5 can be connected to a reference platform 7 of a rack in a pulling manner, so that the incubation bins can be pulled, a slide glass support can be conveniently placed and taken out, and the test convenience and stability are improved; establish buckle mechanism in order to realize the buckle after hatching the storehouse and pushing in the target place as required between hatching storehouse and frame. The incubation seat 502 of the incubation bin is provided with a liquid inlet 504, a liquid outlet 505 and a overflowing liquid port 506 which are communicated with a liquid storage tank 507; the liquid inlet 504 is used for filling liquid into the liquid storage tank 507; a liquid discharge port 505 for discharging liquid from the reservoir 507 as necessary; the overflow port 506 is used to overflow excess liquid in the reservoir 507 as necessary. The slide glass module comprises a slide glass bracket 501 arranged in a liquid storage groove 507, the slide glass bracket 501 is arranged in the liquid storage groove 507, two ends of the slide glass bracket 501 are supported on an incubation seat 502, the slide glass bracket 501 is provided with a plurality of obliquely arranged insertion grooves, the number of the inclined insertion grooves is 16, the slide glass assembly is inserted along the insertion grooves of the slide glass bracket 501, the slide glass assembly comprises a slide glass cover plate 50 and slide glasses 509 (slide glass) attached to the surface of the slide glass cover plate 50, the slide glasses 509 are provided with first identification information, each slide glass 509 is provided with one-to-one corresponding first identification information, and the first identification information is a bar code or a two-dimensional code adhered to the cover plate and used for indicating the pathological number of the slide glass (such as a hospital department and a case number) and the position of the slide glass; as desired, a plurality of slide holders 501 may be disposed in each reservoir, with the plurality of slide holders 501 arranged in an array. Such as a heater plate, mounted to the bottom surface of the reservoir 507 to heat the liquid medium, and the temperature sensor 503 mounted within the reservoir 507 to sense the liquid medium temperature in real time. The control unit adjusts the heating power output of the heaters of the respective incubation bins according to the real-time detection temperature of the temperature sensor 503 of the respective incubation bin, and meanwhile, the liquid medium can be added to cool the temperature of the liquid medium according to the requirement.
The frame is provided with a reference platform 7, the incubation bin module is arranged on the reference platform 7, and the feeding mechanism takes the reference platform 7 as a reference so as to improve the feeding precision. The feeding mechanism comprises an X-axis module 10, a Y-axis module 8 and a Z-axis module 9. The X-axis module 10 comprises an X-axis guide rail, an X-axis driving motor and a belt transmission mechanism, the X-axis guide rail is horizontally arranged on a base body fixedly arranged on the reference platform 7, the belt transmission mechanism comprises a driving wheel, a driven wheel and a belt connected with the driving wheel and the driven wheel, the X-axis driving motor is arranged on the base body, the X-axis driving motor is connected with the driving wheel, the driven wheel is rotatably supported below a Y-axis guide rail of the Y-axis module 8, and the Y-axis guide rail can be driven to slide along the X axis through the X-axis driving motor. The Y-axis module 8 comprises a Y-axis guide rail and a Y-axis stepping driving mechanism, the Z-axis module is connected with the Y-axis guide rail in a sliding mode, and the Y-axis stepping driving mechanism is connected with the Z-axis module so as to drive the Z-axis guide rail of the Z-axis module to slide along the Y axis. The Z-axis module 9 comprises a Z-axis guide rail and a Z-axis stepping control mechanism, the Z-axis stepping control mechanism is connected with the Z-axis guide rail and connected with a sample-adding scanning component which is connected with the Z-axis guide rail in a sliding manner, and the Z-axis stepping control mechanism drives the sample-adding scanning component to move up and down along the Z axis.
The controller unit comprises a plurality of control sheets, the control sheets correspond to the sample processing areas one by one, and the control sheets control the processing procedures of the sample processing areas respectively. In the present embodiment, four control sheets 101, 102, 103, and 104 are provided, the four control sheets 101, 102, 103, and 104 are fixedly disposed on the base body and independently control the a incubation chamber 5, the B incubation chamber 4, the C incubation chamber 3, and the D incubation chamber 2, and the control panels (101 to 104) precisely control the temperatures of the four regions, so as to provide independent test environments for immunohistochemical specimens, and select different procedures (protocols) for each region, usually, the immunohistochemical specimens can select operation procedures according to the required treatment specimen, and the partition cycle management system can solve the problem well, and the four regions do not interfere with each other. The multiple specimen processing areas share the feeding mechanism and the sample adding scanning component, the control unit is connected with the feeding mechanism and the sample adding scanning component, and the sample adding scanning component is conveyed to the required position of each incubation bin through the feeding mechanism, so that the equipment cost is reduced, the occupied space is reduced, and the energy consumption is reduced.
The sample-adding scanning component comprises a sample-adding needle unit 1 and a scanner mechanism. This application of sample needle unit 1 includes an application of sample needle or a plurality of application of sample needle, feeds the application of sample needle through feed mechanism and can reciprocate in order to adjust application of sample needle height promptly with 7 distances of benchmark platform, can control the back-and-forth movement in order to can the automatically move application of sample groove top to slide glass subassembly. The sample adding micropump is also arranged, the sample adding micropump is connected with the sample adding needle unit 1 through a pipeline, the control piece controls the feeding mechanism and conveys the sample adding needle unit to the upper part of a sample adding groove of a slide glass component of each incubation bin slide glass module, the control piece is connected with the sample adding micropump and controls the sample adding micropump through the control unit so as to control the volume and the frequency of sampling and lofting, the sample adding amount of each time of the reagent and one of the multiple samples after one time of reagent is taken and is added or taken, the sample adding precision is high, and the testing precision is high. The scanner mechanism comprises a scanner 6 which can identify first identification information of a slide arranged on a slide module and second identification information of a reagent bottle, wherein the scanner 6 scans the identification information to identify the type of a reagent, the position of the reagent, the pathological number of the slide and the position of the slide so as to control a sample adding needle to extract the reagent and inject the reagent into a sample adding groove; the scanner 6 is connected with the control panel, transmits the scanning result to the corresponding control panel (101-104), and the corresponding processing rule is transferred to the control panel (101-104), and according to the processing rule and according to the detection data, the power output of the heating plate 508 is adjusted, and the rule is selected as required, so that the test precision is high.
The control unit is provided with a plurality of processing protocols which are respectively suitable for different pathological tissue sections, such as A1, A2, A3 and A4.
The immunohistochemical specimen processing method adopting a paraffin section mode comprises the following steps:
and 3, processing the sample processing area by the control unit according to the called processing rule until the processing is finished, and executing the step 1 in the execution process of the step 3.
After a slide rack to be tested is placed in the incubation bin A, the feeding mechanism starts to move, the bar code information on the slide specimen in the incubation bin A is identified, then a test procedure in the control unit is selected according to the type and the requirement of the pathological slide specimen (for example, the procedure of A1 is selected), and the step of running the procedure of A1 is started, which is called as thread 1: dewaxing, hydration, antigen retrieval, endogenous enzyme blocking, primary anti-incubation, secondary antibody incubation, color development, hematoxylin counterstaining, transparence and mounting, if the slide is processed, a group of slide supports to be tested are loaded in an incubation bin B, after the operation is started, a thread 2 starts to operate, a feeding mechanism can be switched to be controlled by the thread 2 at the interval of the operation step of the thread 1 (namely the time for waiting for the reagent to act), the task of identifying the bar code information on the slide specimen in the incubation bin B is executed, then a test procedure in control software is selected according to the type and the requirement of the pathological slide specimen (such as selection of an A2 procedure), the step of operating an A2 procedure is started, the feeding mechanism is arranged between the two sub-areas of the incubation bin A and the incubation bin B, and the step of one procedure is executed in the waiting time for finishing the execution of the other procedure. All the steps of the A1 protocol and the A2 protocol corresponding to the A incubation chamber and the B incubation chamber are performed in sequence.
The treatment protocol is provided with a priority procedure, such as color development and secondary antibody incubation; in the process of executing steps 2 and 3, if a sample loading scanning component is applied to a certain sample processing area (supposing A) according to the processing protocol, the following steps are executed: step A, judging whether the application of the sample-adding scanning component is in a standby state, if so, applying, otherwise, executing step B; step B, judging whether the sample processing area A is subjected to a color development or secondary antibody incubation process by using a sample adding scanning component, if so, executing step C1, otherwise, executing step C2; step C1, applying the sample-adding scanning component in the sample processing area A according to the processing rule, and continuing to execute the original application after the sample-adding scanning component is used; step C2, wait until in standby state and mark as standby state after application is completed.
The above description is only a preferred embodiment of the present invention, and therefore should not be taken as limiting the scope of the invention, which is defined by the appended claims and their equivalents.
Claims (12)
1. The immunohistochemical specimen processing device adopting the paraffin section mode comprises a rack, an incubation bin module, a feeding mechanism, a sample adding scanning component and a control unit, wherein the incubation bin module and the feeding mechanism are arranged on the rack, and the sample adding scanning component is connected with the feeding mechanism; the method is characterized in that: the incubation bin module comprises a plurality of specimen processing areas, each specimen processing area comprises at least one incubation bin, each incubation bin comprises an incubation seat, each incubation seat is provided with a liquid storage tank for storing liquid medium, a heater for heating the liquid medium and a temperature sensor for detecting the temperature of the liquid medium, a slide glass module with a slide glass is placed in the liquid storage tank, and the control unit is connected with the heater and the temperature sensor of each incubation bin and independently controls each specimen processing area to process the specimen in a partitioned mode; the incubation bin module has a plurality of sample processing areas sharing a feeding mechanism and a sample adding scanning component, and the control unit is connected with the feeding mechanism and the sample adding scanning component and conveys the sample adding scanning component to the required position of each incubation bin through the feeding mechanism.
2. The immunohistochemical specimen processing device according to claim 1, wherein: this hatch a plurality of storehouses of hatching of storehouse module and arrange and hatch storehouse side by side and can pull connection frame.
3. The immunohistochemical specimen processing device according to claim 1, wherein: the controller unit includes a plurality of control pieces that correspond to the plurality of sample processing areas one-to-one and control the heaters and the temperature sensors of the respective sample processing areas, respectively.
4. The immunohistochemical specimen processing device according to claim 1, wherein: each sample processing region includes an incubation compartment.
5. The immunohistochemical specimen processing device according to claim 1, wherein: the sample-loading scanning component comprises a sample-loading needle unit and a scanner mechanism, the scanner mechanism comprises a scanner which can at least identify the identification information of a slide glass arranged on the slide glass module, and the control unit controls the corresponding sample processing area according to the information obtained by scanning the identification information by the scanner.
6. The immunohistochemical specimen processing device according to claim 1, wherein: the slide module comprises a slide bracket, a slide assembly is placed on the slide bracket, and the slide assembly comprises a slide and a slide cover plate; the slide glass bracket is placed in the liquid storage tank, two ends of the slide glass bracket are supported on the incubation seat, the slide glass bracket is provided with a plurality of obliquely arranged insertion grooves, and the slide glass assembly is inserted along the slide glass bracket insertion grooves.
7. The immunohistochemical specimen processing device according to claim 1, wherein: the heater is installed on the bottom surface of the liquid storage tank, the temperature sensor is installed in the liquid storage tank, and the control unit adjusts the heating power output of the incubation bin heater according to the real-time detection temperature of the incubation bin temperature sensor.
8. The immunohistochemical specimen processing device according to claim 1, wherein: the device comprises a control unit, a sample loading scanning component, a sample loading groove, a sample loading micro-pump, a sample loading scanning component, a sample loading groove and a sample unloading groove, wherein the sample loading micro-pump is connected with the sample loading needle unit of the sample loading scanning component through a pipeline, the control unit controls a feeding mechanism and conveys the sample loading needle unit to the sample loading groove top of the slide glass component of each incubation bin slide glass module, and the control unit is connected with the sample loading micro-pump and controls the volume and the frequency of sampling and lofting through the control unit.
9. The immunohistochemical specimen processing device according to claim 1, wherein: the frame is provided with a reference platform, the incubation bin module is arranged on the reference platform, and the feeding mechanism takes the reference platform as a reference.
10. The immunohistochemical specimen processing method adopting the paraffin section mode is characterized by comprising the following steps of:
the immunohistochemical specimen processing device comprises an incubation bin module, a sample adding scanning component and a control unit, wherein the incubation bin module comprises a plurality of specimen processing areas, each specimen processing area comprises at least one incubation bin, and the control unit is connected with each incubation bin and independently controls each specimen processing area to process a specimen in a partition manner; the control unit stores a plurality of treatment protocols which are respectively suitable for different pathological tissue sections, the treatment protocols comprise heating temperature and heating time, and the control unit is connected with the sample-adding scanning component;
the processing method comprises the following steps:
step 1, judging whether a specimen processing area needs to be processed, if so, executing step 2, otherwise, continuing to execute the step;
step 2, the sample-adding scanning component scans the identification information of the slide glass assembly in the sample processing area, and the control unit calls a corresponding processing rule according to the identification information and then executes step 3;
and 3, processing the sample processing area by the control unit according to the called processing rule until the processing is finished, and executing the step 1 in the execution process of the step 3.
11. The method for processing an immunohistochemical specimen by paraffin section according to claim 10, wherein: the immunohistochemical sample processing device comprises a feeding mechanism, wherein the sample adding scanning component is connected with the feeding mechanism, a plurality of sample processing areas of the incubation cabin module share the feeding mechanism and the sample adding scanning component, and the control unit is connected with the feeding mechanism and the sample adding scanning component and conveys the sample adding scanning component to the required position of each incubation cabin through the feeding mechanism;
and (3) in the process of executing the steps 2 and 3, if a sample-adding scanning component is to be applied to a certain sample processing area according to the processing protocol, judging whether the application of the sample-adding scanning component is in a standby state, if so, applying, otherwise, waiting until the application is in the standby state, and marking the application as the standby state after the application is finished.
12. The method for processing an immunohistochemical specimen using a paraffin section method according to claim 11, wherein:
setting a priority procedure for the treatment procedure; in the process of executing steps 2 and 3, if a sample-loading scanning component is applied to a certain sample processing area according to a processing protocol, executing the following steps:
step A, judging whether the application of the sample-adding scanning component is in a standby state, if so, applying, otherwise, executing step B;
step B, judging whether the sample processing area applies the sample adding scanning component to be a priority process, if so, executing step C1, otherwise, executing step C2;
step C1, the sample processing area applies the sample-adding scanning component according to the processing rule, and continues to execute the original application after the use;
step C2, wait until in standby state and mark as standby state after application is completed.
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