CN216718450U - Multichannel slide specimen cleaning mechanism and immunohistochemical specimen processor - Google Patents
Multichannel slide specimen cleaning mechanism and immunohistochemical specimen processor Download PDFInfo
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- CN216718450U CN216718450U CN202123032063.XU CN202123032063U CN216718450U CN 216718450 U CN216718450 U CN 216718450U CN 202123032063 U CN202123032063 U CN 202123032063U CN 216718450 U CN216718450 U CN 216718450U
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Abstract
The utility model discloses a multichannel slide specimen cleaning mechanism and an immunohistochemical specimen processor, wherein the multichannel slide specimen cleaning mechanism comprises an integrated base, at least one liquid inlet branch and a plurality of liquid adding heads, an integrated cavity is arranged in the integrated base, a filtering device is arranged on the liquid inlet branch and communicated with the integrated cavity, the plurality of liquid adding heads are arranged on the integrated base side by side and communicated with the integrated cavity, and the plurality of liquid adding heads arranged side by side correspond to a row of slide assemblies of a slide support one by one. It has the following advantages: the filtered cleaning solution can be used for filling a row of slide glass components at one time, so that the filling efficiency is improved, the cleaning efficiency is improved, the testing time of the immunohistochemical whole flow is greatly shortened, the accurate control requirement of the immunohistochemical key link time is ensured, and a good dyeing result is easier to obtain.
Description
Technical Field
The utility model relates to the technical field of diagnostic medical instruments, in particular to a multi-channel slide specimen cleaning mechanism and an immunohistochemical specimen processor.
Background
The immunohistochemical specimen treatment is a complex process, and relates to multiple steps and links such as dewaxing and hydration, antigen retrieval, primary antibody incubation, synergism, secondary antibody incubation, color development, counterstaining, acidification, blue returning and the like. In the instrument for processing the immunohistochemical specimen in the market at present, a liquid adding device is shared in each step of sample adding and cleaning, the resource occupation conflict of each step on the liquid adding device is serious, when the resource of the liquid adding device is occupied, other steps can be carried out by sample adding operation of other steps and links only after the current link is finished, on one hand, the test time of one immunohistochemical flow is prolonged, and the efficiency is reduced; on the other hand, the accurate control of the time of the key links of immunohistochemistry, especially the key links of tissue repair, antibody incubation and the like, is influenced, and the excessive time causes over-test, thereby influencing the test effect.
SUMMERY OF THE UTILITY MODEL
The utility model provides a multi-channel slide specimen cleaning mechanism and an immunohistochemical specimen processor, which overcome the defects of the immunohistochemical specimen processor in the background art.
One of the technical schemes adopted by the utility model for solving the technical problems is as follows: multichannel slide glass sample wiper mechanism, its characterized in that: including an integrated seat, an at least feed liquor branch road and a plurality of liquid feeding head, establish the integrated chamber in this integrated seat, establish filter equipment and switch-on integrated chamber on this feed liquor branch road, this a plurality of liquid feeding heads set up side by side at integrated seat and all switch-on integrated chamber, a plurality of liquid feeding heads that set up side by side and a plurality of slide glass subassembly one-to-one of a row of slide glass support.
In one embodiment: the liquid inlet branch circuits are arranged in a plurality and are connected in parallel to the integrated cavity.
In one embodiment: a plurality of liquid outlets arranged side by side are arranged at the bottom of the integrated base, and the plurality of liquid adding heads are respectively arranged at the plurality of liquid outlets.
In one embodiment: the top of the integrated seat is provided with a plurality of liquid inlets which are arranged side by side, and each liquid inlet is provided with the filtering device.
The second technical scheme adopted by the utility model for solving the technical problems is as follows: the immunohistochemical specimen processor comprises a cleaning device, and the cleaning device comprises the multichannel slide specimen cleaning mechanism.
In one embodiment: still include the frame, incubate storehouse module and the control unit, should incubate the storehouse module and include a plurality of sample treatment area, every sample treatment area includes at least one above-mentioned storehouse of incubating, should incubate the storehouse including incubating the seat, should incubate the seat and establish the reservoir of stock solution body medium, the heater of heating fluid medium and the temperature sensor who detects the liquid medium temperature, the heater and the temperature sensor in each storehouse and each sample treatment area of independent control are hatched the subregion and are handled the sample in this the control unit connection.
Compared with the background technology, the technical scheme has the following advantages:
the wiper mechanism includes a plurality of liquid feeding heads, filter equipment is established to the feed liquor branch road, a plurality of liquid feeding heads set up side by side at integrated seat and all put through integrated chamber, a plurality of liquid feeding heads that set up side by side and a plurality of slide glass subassembly one-to-one of a row of slide glass support, the cleaning solution after the filtration can once annotate a row of slide glass subassembly promptly, improve the filling efficiency, the cleaning efficiency is improved, greatly shorten the test time of immunohistochemical full flow, the immunohistochemical sample treatment effeciency has not only been effectively improved, the control accuracy of immunohistochemical step to the time has been improved simultaneously, the dyeing quality of sample has been improved.
The feed liquor branch road establishes a plurality of parallelly connected settings, promotes feed liquor speed, promotes the cleaning efficiency.
Drawings
The utility model is further described with reference to the following figures and detailed description.
Fig. 1 is a schematic perspective view of an immunohistochemical specimen processor according to an embodiment.
Fig. 2 is a second perspective view of the immunohistochemical specimen processor according to the embodiment.
Fig. 3 is a schematic perspective view of a feeding mechanism according to an embodiment.
Fig. 4 is a schematic perspective view of an embodiment of a slide holder.
Fig. 5 is a schematic diagram of a cleaning liquid path of the cleaning apparatus according to the embodiment.
FIG. 6 is a schematic structural view of a cleaning mechanism according to an embodiment.
Detailed Description
Referring to fig. 1 to 6, the multi-channel slide specimen cleaning apparatus includes a control board 107, a liquid inlet pump 105, a pressure control valve 104, an electromagnetic valve 106 and a cleaning mechanism, wherein an inlet of the liquid inlet pump 105 is connected to a liquid storage barrel 108 for storing a cleaning liquid, the electromagnetic valve 106 is disposed between an outlet of the liquid inlet pump 105 and the cleaning mechanism, one port of the pressure control valve 104 is connected between the outlet of the liquid inlet pump 105 and the electromagnetic valve 106, and the other port is connected to the liquid storage barrel 108. This wiper mechanism includes integrated seat 102, a plurality of feed liquor branch circuits and a plurality of liquid feeding head 103 that connect in parallel and set up, establishes the integrated chamber in this integrated seat 102, and this a plurality of feed liquor branch circuits link up and all establish filter equipment 101 between solenoid valve 106 export and integrated chamber and on each feed liquor branch circuit, and this a plurality of liquid feeding heads 103 set up side by side at integrated seat 102 and all switch on the integrated chamber, a plurality of liquid feeding head 103 that set up side by side and a plurality of slide glass assembly 201 one-to-one of a row of slide glass support. The control board 107 controls the starting and stopping of the liquid inlet pump 105 and controls the opening and closing of the electromagnetic valve 106; the pressure control valve 104 is used for limiting the highest pressure of a pipeline between the outlet of the liquid inlet pump 105 and the inlet of the electromagnetic valve 106; the filtering device 101 is installed on a liquid filling port at the top of the integration seat 102 and is used for filtering the cleaning liquid entering the integration seat 102 and ensuring the cleanliness of the liquid entering the integration seat 102. In the concrete structure: the top wall of the integrated seat 102 is provided with a plurality of liquid inlets which are connected in parallel with an electromagnetic valve 106 through pipelines; the bottom of the integrated base 102 is provided with a plurality of liquid outlets arranged side by side, and the plurality of liquid adding heads 103 are respectively arranged at the plurality of liquid outlets.
Adopt immunohistochemical sample treater of paraffin section mode, which comprises a frame, hatch a storehouse module, feed mechanism, application of sample scanning component and control unit, should hatch a storehouse module, feed mechanism all installs in the frame, feed mechanism is connected to this application of sample scanning component, this hatch a storehouse module and includes a plurality of sample treatment areas, each sample treatment area corresponds one and hatches the storehouse, this a plurality of storehouse structure of hatching are the same and including hatching seat 502, should hatch seat 502 and establish the reservoir of liquid body medium, the heater of liquid body medium and the temperature sensor who detects the liquid medium temperature, the built-in slide glass support of putting the tape carrier subassembly of this reservoir. The control unit is connected with the heaters and the temperature sensors of the incubation bins and independently controls the specimen processing areas to process specimens in a partitioning mode, each area can select processing procedures according to pathological slide specimens, and processing steps of different processing procedures or/and temperature control temperature or/and heating time and the like are different so as to ensure that each specimen is processed according to required procedures to obtain an optimal test result, (1) partitioning cycle management is adopted, the normal use requirement of immunohistochemistry can be met, the specimens can be processed in a partitioning mode and in batches, and the immunohistochemistry specimen processing efficiency is effectively improved; (2) the slide glass specimens which need different operation flows can be processed in different environment areas, and the test result is improved; (3) after operation of a certain region is finished, secondary specimen loading can be carried out in the region, and the treatment capacity of the immunohistochemical specimen is greatly improved. The sample-loading scanning component comprises the multi-channel slide specimen cleaning device and a scanner mechanism.
The slide glass assembly 201 comprises a slide glass cover plate and slide glasses (slide glass) attached to the surface of the slide glass cover plate, wherein the slide glasses are provided with first identification information, each slide glass is provided with one-to-one corresponding first identification information, and the first identification information is a bar code or a two-dimensional code which is adhered to the cover plate and used for indicating a pathology number (such as a hospital department and a case number) of the slide glass and the position of the slide glass; according to the requirement, a plurality of slide glass brackets can be placed in each liquid storage tank, and the slide glass brackets are arranged. The heater, such as a heating plate, is mounted to the bottom surface of the reservoir to heat the liquid medium, and the temperature sensor is mounted within the reservoir to sense the temperature of the liquid medium in real time. The control unit detects the temperature in real time according to the temperature sensor of each incubation bin, adjusts the heating power output of the heater of each incubation bin, and can increase the liquid medium to cool the temperature of the liquid medium according to the requirement.
The controller unit comprises a plurality of control sheets, the control sheets correspond to the sample processing areas one by one, and the control sheets control the processing procedures of the sample processing areas respectively. Among this embodiment, establish four control pieces 107, four control pieces 107 set firmly and respectively independent control A at the base member and incubate the storehouse, B incubate the storehouse, C incubate the storehouse, D incubates the storehouse, the control panel realizes accurate control to four regional temperatures, thereby for immunohistochemical sample provides independent test environment, and can select different processes (regulations) to every region, usually immunohistochemical sample can be according to required processing standard originally selection operation flow, and this problem can be well solved to subregion circulation management system, four regional mutual noninterferences. The multiple specimen processing areas share the feeding mechanism and the sample adding scanning component, the control unit is connected with the feeding mechanism and the sample adding scanning component, and the sample adding scanning component is conveyed to the required position of each incubation bin through the feeding mechanism, so that the equipment cost is reduced, the occupied space is reduced, and the energy consumption is reduced. The control unit is provided with a plurality of processing protocols which are respectively suitable for different pathological tissue sections, such as A1, A2, A3 and A4.
The scanner mechanism comprises a scanner which can identify first identification information of a slide glass arranged on the slide glass module and second identification information arranged on the reagent bottle, wherein the scanner scans the identification information to identify the type of a reagent, the position of the reagent, the pathological number of the slide glass and the position of the slide glass; the scanner is connected with the control panel, transmits the scanning result to the corresponding control panel, and the control panel calls the corresponding processing rule, adjusts the power output of the heating plate according to the processing rule and the detection data, selects the rule as required, and has high testing precision.
The frame is provided with a reference platform 7, the incubation bin module is arranged on the reference platform 7, and the feeding mechanism takes the reference platform as a reference so as to improve the feeding precision. The feeding mechanism comprises an X-axis module 10, a Y-axis module 8 and a Z-axis module 9. The X-axis module 10 comprises an X-axis guide rail, an X-axis driving motor 11 and a belt transmission mechanism, the X-axis guide rail is horizontally arranged on a base body fixedly arranged on the reference platform 7, the belt transmission mechanism comprises a driving wheel, a driven wheel and a belt connected with the driving wheel and the driven wheel, the X-axis driving motor is arranged on the base body, the X-axis driving motor is connected with the driving wheel, the driven wheel is rotatably supported below a Y-axis guide rail of the Y-axis module 8, and the Y-axis guide rail can be driven to slide along the X axis through the X-axis driving motor. The Y-axis module 8 comprises a Y-axis guide rail and a Y-axis stepping driving mechanism 81, the Z-axis module is connected with the Y-axis guide rail in a sliding manner, and the Y-axis stepping driving mechanism is connected with the Z-axis module so as to drive the Z-axis guide rail of the Z-axis module to slide along the Y axis. The Z-axis module 9 comprises a Z-axis guide rail and a Z-axis stepping control mechanism, and the Z-axis stepping control mechanism is assembled and connected with the Z-axis guide rail. The integrated seat 102 of the multi-channel slide specimen cleaning device is mounted on a Y-axis guide rail so as to drive the integrated seat 102 to move to a cleaning position through an X-axis module and a Y-axis module; the scanner mechanism is slidably connected to the Z-axis guide rail, and the Z-axis step control mechanism drives the scanner mechanism to move up and down along the Z-axis, so as to drive the integration seat 102 to move to the working position through the X-axis module, the Y-axis module and the Z-axis module.
Under the control of the feeding mechanism, the integrated base 102 and the liquid adding head 103 move to the upper part of the slide glass assembly 201, the electromagnetic valve 106 and the liquid inlet pump 105 are simultaneously opened, the cleaning liquid with preset pressure is filtered by the filtering device 101 and then is divided into multiple paths by the integrated base 102, the cleaning liquid is simultaneously added to the whole column of the slide glass assembly 201 through the liquid adding head 103, and the adding time is precisely controlled by the control board 107.
In the whole immunohistochemical test process, the cleaning of the slide glass specimen is independently and accurately controlled by the control board 107, and manual operation is not needed. The device has realized that the immunohistochemistry washs fast between different link steps, has solved the resource conflict problem of washing to the liquid feeding device between each step application of sample of immunohistochemistry and different link steps, has greatly shortened the test time of immunohistochemistry overall flow, has guaranteed the accurate control requirement of immunohistochemistry key link time simultaneously, changes in obtaining good dyeing result.
The cleaning method of the immunohistochemical specimen processor comprises the following steps:
step 1, receiving a cleaning instruction;
step 2, the feeding mechanism drives the integrated seat to move to a cleaning position, and the integrated seat of the cleaning device moves to the position above the row of slide specimen assemblies;
step 3, the terminal sends a command to the control panel to control the liquid inlet pump and the electromagnetic valve to be opened simultaneously, cleaning liquid passes through the liquid inlet pump, the electromagnetic valve, the liquid inlet branch, the filtering device, the liquid inlet cavity and the liquid adding heads, and the cleaning liquid sprayed by the liquid adding heads respectively fills the cleaning liquid into the slide glass assemblies;
and 4, after the filling, controlling the liquid inlet pump and the electromagnetic valve to stop by the control panel, moving the integrated base to the position above the next target column slide specimen along with the feeding mechanism, and repeating the steps 2-3.
According to the specific implementation mode, rapid cleaning among different immunohistochemical link steps is realized, the problem of resource conflict of the liquid adding device caused by sample adding of each immunohistochemical step and cleaning among different link steps is solved, the testing time of the whole immunohistochemical process is greatly shortened, the accurate control requirement of the immunohistochemical key link time is guaranteed, and a good dyeing result is easier to obtain.
The above description is only a preferred embodiment of the present invention, and therefore should not be taken as limiting the scope of the utility model, which is defined by the appended claims and their equivalents.
Claims (6)
1. Multichannel slide glass sample wiper mechanism, its characterized in that: including an integrated seat, an at least feed liquor branch road and a plurality of liquid feeding head, establish the integrated chamber in this integrated seat, establish filter equipment and switch-on integrated chamber on this feed liquor branch road, this a plurality of liquid feeding heads set up side by side at integrated seat and all switch-on integrated chamber, a plurality of liquid feeding heads that set up side by side and a plurality of slide glass subassembly one-to-one of a row of slide glass support.
2. The multi-channel slide specimen cleaning mechanism of claim 1, wherein: the liquid inlet branch circuits are arranged in a plurality and are connected in parallel to the integrated cavity.
3. The multi-channel slide specimen cleaning mechanism of claim 1, wherein: a plurality of liquid outlets arranged side by side are arranged at the bottom of the integrated base, and the plurality of liquid adding heads are respectively arranged at the plurality of liquid outlets.
4. The multi-channel slide specimen cleaning mechanism of claim 1, wherein: the top of the integrated base is provided with a plurality of liquid inlets which are arranged side by side, and each liquid inlet is provided with the filtering device.
5. Immunohistochemical sample treater which characterized in that: comprising a cleaning device comprising the multi-channel slide specimen cleaning mechanism of claim 1.
6. The immunohistochemical specimen processor of claim 5, wherein: still include the frame, incubate storehouse module and the control unit, should incubate the storehouse module and include a plurality of sample treatment area, every sample treatment area includes at least one above-mentioned storehouse of incubating, should incubate the storehouse including incubating the seat, should incubate the seat and establish the reservoir of stock solution body medium, the heater of heating fluid medium and the temperature sensor who detects the liquid medium temperature, the heater and the temperature sensor in each storehouse and each sample treatment area of independent control are hatched the subregion and are handled the sample in this the control unit connection.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8758707B2 (en) * | 2008-06-16 | 2014-06-24 | Leica Biosystems Melbourne Pty Ltd | Staining instruments and methods |
| CN102794273B (en) * | 2012-08-10 | 2014-09-24 | 深圳德夏科技发展有限公司 | Plate washing machine cleaning head and plate washing machine assembly |
| CN105675369B (en) * | 2016-03-21 | 2019-02-01 | 厦门通灵生物医药科技有限公司 | A kind of device and method handling biological slide sample |
| CN106623236B (en) * | 2016-09-19 | 2019-03-29 | 嘉兴科瑞迪医疗器械有限公司 | A kind of board-washing machine |
| CN106733827B (en) * | 2016-12-07 | 2019-05-28 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of ELIAS microplate washer Anti-overflow device |
| CN108254235B (en) * | 2018-01-10 | 2020-03-17 | 福州大学 | Array type sample injector |
| CN209680659U (en) * | 2019-02-13 | 2019-11-26 | 广州华银健康科技有限公司 | A kind of high efficiency plate cleaning machine cleaning device |
| CN213085463U (en) * | 2020-09-11 | 2021-04-30 | 深圳市奥美顿科技有限公司 | Automatic multichannel liquid separation appearance of changing |
| CN213715249U (en) * | 2020-11-16 | 2021-07-16 | 河南新网检测服务有限公司 | Sample adding equipment for component measurement |
| CN213793361U (en) * | 2020-11-17 | 2021-07-27 | 漳州卫生职业学院 | Small cover glass cleaning device |
| CN214585491U (en) * | 2021-03-22 | 2021-11-02 | 无锡华卫德朗仪器有限公司 | Modular plate washing machine of full-automatic enzyme-linked immunoassay analyzer |
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