CN114152496A - Automatic immunohistochemical dyeing machine - Google Patents
Automatic immunohistochemical dyeing machine Download PDFInfo
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- CN114152496A CN114152496A CN202111667389.1A CN202111667389A CN114152496A CN 114152496 A CN114152496 A CN 114152496A CN 202111667389 A CN202111667389 A CN 202111667389A CN 114152496 A CN114152496 A CN 114152496A
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- 238000004043 dyeing Methods 0.000 title claims abstract description 23
- 230000002055 immunohistochemical effect Effects 0.000 title claims abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 175
- 239000007788 liquid Substances 0.000 claims abstract description 80
- 238000011534 incubation Methods 0.000 claims abstract description 57
- 230000007246 mechanism Effects 0.000 claims abstract description 15
- 238000005086 pumping Methods 0.000 claims abstract description 10
- 238000012545 processing Methods 0.000 claims description 32
- 238000011532 immunohistochemical staining Methods 0.000 claims description 28
- 238000010438 heat treatment Methods 0.000 claims description 13
- 238000005485 electric heating Methods 0.000 claims description 6
- 238000005057 refrigeration Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 16
- 230000008569 process Effects 0.000 abstract description 12
- 239000000523 sample Substances 0.000 description 56
- 239000011521 glass Substances 0.000 description 31
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- 230000002349 favourable effect Effects 0.000 description 7
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- 239000000427 antigen Substances 0.000 description 3
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- 239000003795 chemical substances by application Substances 0.000 description 2
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- 241000274582 Pycnanthus angolensis Species 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
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- 238000010586 diagram Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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Abstract
The invention relates to an automatic immunohistochemical dyeing machine which comprises a rack, wherein a mechanical arm is arranged on the rack, a reagent bin and an incubation bin are arranged on the rack, the mechanical arm comprises a sample adding needle, an auxiliary reagent liquid flow vehicle is arranged on the rack, the auxiliary reagent liquid flow vehicle comprises a liquid flow vehicle moving seat which is assembled on the rack in a back-and-forth moving mode, and the liquid flow vehicle moving seat is positioned on one side of the incubation bin in the left-and-right direction; an auxiliary reagent filling needle is arranged on the liquid flow vehicle moving seat and connected with an auxiliary reagent pumping device arranged on the rack; the frame is provided with a liquid flow vehicle moving seat driving mechanism for driving the liquid flow vehicle moving seat to move back and forth; at least two incubation bins are arranged along the left and right direction, each incubation bin is correspondingly provided with an auxiliary reagent liquid flow vehicle, and the at least two incubation bins share one mechanical arm; the mechanical arm and the auxiliary reagent liquid flow vehicle can independently operate, different steps in the dyeing process can be synchronously carried out, the waiting time is shortened, and the treatment efficiency is improved.
Description
Technical Field
The invention relates to the technical field of equipment for preparing samples, in particular to immunohistochemical dyeing equipment, and particularly relates to an automatic immunohistochemical dyeing machine.
Background
Immunohistochemistry, which is a basic principle of antigen-antibody specific binding in immunology, is a research on the localization, characterization and relative quantification of an antigen in a tissue cell by developing a color-developing agent of a labeled antibody through a chemical reaction, and is widely used in the fields of cell biology, microbiology, clinical diagnosis and the like.
When a biological sample is processed, the biological sample needs to be placed on a glass slide, a very thin reaction cavity is formed by matching a liquid cover film and the glass slide, and reaction liquid is added into one end of the reaction cavity to enable the biological sample to be fully reacted with the reaction liquid, and the operations can be realized by biological sample processing equipment at present, for example, a top cover plate assembly and the biological sample processing equipment disclosed by a patent document with an authorization publication number of CN111896350B, the glass slide and the liquid cover film carrying the biological sample are placed on a slide carrying disc, and after the carrying disc is placed into the equipment, the liquid cover film is pressed by a pressing unit on the top cover plate, so that the slide is pressed, and the subsequent sample processing process can be carried out.
In the immunohistochemical staining process, operations such as dewaxing, antigen retrieval, antigen-antibody reaction or biological sample incubation need to be performed on a biological sample, the steps are complicated, and especially in the case of a large-flux experiment with a large number of samples, the whole staining process consumes a long time.
In order to reduce time consumption, there is a technology for dyeing a biological sample by a full-automatic immunohistochemical staining machine, for example, a full-automatic immunohistochemical staining machine disclosed in patent document No. CN208705146U, in which a mechanical arm drives a sample adding mechanism and a spray air-drying mechanism to move freely in a three-dimensional space, the sample adding mechanism includes a sample adding needle, and the spray air-drying mechanism includes a spray needle, and can automatically add, spray or air-dry a pathological tissue on a slide in the immunohistochemical staining machine, so that the immunohistochemical staining machine can realize functions of automatic dewaxing and antigen retrieval, and the automation degree of the immunohistochemical staining machine is improved; for example, in the fully-automatic immunohistochemical staining apparatus disclosed in patent application publication No. CN111323287A, a double-needle pipetting arm can move in an X/Y/Z free space, a sample injection needle accurately moves right above a glass slide to perform reagent drip irrigation, and two sample injection needles are installed and arranged on a mechanical arm, so that the sampling and sample injection efficiencies are improved; an immunohistochemical staining machine and an immunohistochemical staining method are disclosed in patent application publication No. CN110411810A, which move a tissue slide and reagents used by arranging a three-dimensional mechanical arm and can realize the ready preparation of the required reagents in the tissue slide staining process.
Although the full-automatic immunohistochemical staining machine can reduce time consumption to a certain extent, in actual use, the mechanical arm of the machine is required to be used for adding an antibody, a staining agent and an auxiliary reagent, the auxiliary reagent is a reagent for auxiliary staining such as a dewaxing reagent, a repairing reagent and a cleaning solution for cleaning slides, which is required in sample treatment and staining processes, when the slides are treated one by one, a large amount of waiting time is generated in the repeated action process of the mechanical arm, the whole staining operation time is still long, and the efficiency is low.
Disclosure of Invention
The invention aims to provide an automatic immunohistochemical dyeing machine to solve the problem of low efficiency of the conventional automatic immunohistochemical dyeing machine.
The technical scheme of the automatic immunohistochemical staining machine is as follows:
an automatic immunohistochemical dyeing machine comprises a rack, wherein a mechanical arm is arranged on the rack, the mechanical arm extends in the front-back direction and is movably arranged on the rack in the left-right direction, a reagent bin for storing a reagent box and an incubation bin for placing a slide are arranged on the rack, the mechanical arm comprises a sample adding needle for adding a reagent in the reagent bin onto the slide, an auxiliary reagent liquid flow vehicle is arranged on the rack, the auxiliary reagent liquid flow vehicle comprises a liquid flow vehicle moving seat which is assembled on the rack in a front-back movement mode, and the liquid flow vehicle moving seat is positioned on one side of the incubation bin in the left-right direction; an auxiliary reagent filling needle is arranged on the liquid flow vehicle moving seat and connected with an auxiliary reagent pumping device arranged on the rack; the frame is provided with a liquid flow vehicle moving seat driving mechanism for driving the liquid flow vehicle moving seat to move back and forth; incubate the storehouse and be provided with two at least along left right direction, each is incubated the storehouse and all is correspondingly furnished with auxiliary reagent liquid flow car, and two at least arms of incubating the storehouse sharing are hatched to the storehouse.
Has the advantages that: the auxiliary reagent liquid flow vehicle is configured for each incubation bin and is specially used for adding auxiliary reagents, so that the mechanical arm does not need to add the auxiliary reagents, the mechanical arm and the auxiliary reagent liquid flow vehicle can independently operate, different steps in the dyeing process can be synchronously performed, waiting time caused by repeated use of the mechanical arm can be greatly reduced, the total operation time is greatly shortened, and the processing efficiency is improved; simultaneously, through setting up two at least hatching storehouses, can improve the dyeing flux of automatic immunohistochemical dyeing machine.
Further, the mechanical arms are arranged at least two in the left and right, the reagent bin is arranged between the two adjacent mechanical arms, and the incubation bins sharing one mechanical arm are arranged on the same side of the reagent bin.
Has the advantages that: by arranging at least two mechanical arms for liquid adding, more samples can be processed in the same time, and the processing speed is improved; moreover, each incubation bin positioned on the same side of the reagent bin shares one mechanical arm, so that the mutual interference of actions among the mechanical arms can be avoided, and the improvement of the operation efficiency is facilitated.
Furthermore, a mechanical arm moving guide rail extending in the left-right direction is arranged on the rack, the mechanical arm comprises a mechanical arm moving seat, the mechanical arm moving seat is mounted on the mechanical arm moving guide rail, a mechanical arm driving mechanism driving the mechanical arm moving seat to move left and right on the mechanical arm moving guide rail is arranged on the rack, and the mechanical arms share the mechanical arm moving guide rail.
Has the advantages that: each arm sharing arm removes the guide rail, and the convenient setting is favorable to reducing equipment manufacturing cost.
Furthermore, the front end and the rear end of the mechanical arm are both assembled on the frame in a left-right guiding and moving mode.
Has the advantages that: the gravity of the mechanical arm is shared and borne by the two ends, and the reliability and the stability of the operation of the mechanical arm are improved.
Further, the liquid flow vehicle moving seat comprises a seat body and a lifting seat arranged on the seat body, and the auxiliary reagent filling needle is arranged on the lifting seat.
Has the advantages that: the auxiliary reagent filling needle can be lifted, so that when the auxiliary reagent is required to be filled, the auxiliary reagent filling needle can be lowered to be close to the slide, the auxiliary reagent is accurately added onto the slide, and splashing around is avoided.
Further, the auxiliary reagent pumping device is also connected with the sample adding needle on the mechanical arm.
Has the advantages that: make the application of sample needle on the arm not only can add on the slide by the reagent in the reagent storehouse like this, can also add auxiliary reagent to the slide through auxiliary reagent pumping device, conveniently allot the operation action of arm, be favorable to make full use of arm, shorten latency.
Further, the bottom of the reagent cabin is provided with a refrigeration module.
Has the advantages that: the inside of the whole reagent bin can be in a low-temperature state for a long time, so that the reagent can be kept fresh for a long time without going bad, and the dyeing effect is ensured.
Furthermore, a layered sample processing chamber and an auxiliary reagent supply chamber positioned on the lower side of the sample processing chamber are arranged on the rack, the mechanical arm, the reagent chamber and the incubation chamber are all arranged in the sample processing chamber, and the auxiliary reagent pumping device is arranged in the auxiliary reagent supply chamber.
Has the advantages that: the storehouse is arranged on the rack in a layering mode, the arrangement is compact, the occupied area of equipment is favorably reduced, and meanwhile, the sample processing storehouse is arranged above the storehouse, so that the operation of personnel is facilitated.
Furthermore, a sample processing chamber is arranged on the rack, a heating constant temperature module used for keeping the temperature of the sample processing chamber is arranged in the sample processing chamber, and the heating constant temperature module comprises an electric heating assembly and a fan used for sending heat generated by the electric heating assembly into the sample processing chamber.
Has the advantages that: keep warm through heating constant temperature module, also can keep each incubation storehouse ambient temperature in chilly winter, guarantee the activity of reagent, guarantee that reagent dyeing quality is not influenced by external environment temperature.
Furthermore, a slide glass carrying disc is arranged in the incubation bin, the slide glass carrying disc is a single-row slide glass carrying disc, and slide glass mounting positions on the single-row slide glass carrying disc are arranged along the front-back direction so as to enable the slide glasses to be arranged in a row along the front-back direction after the slide glasses are placed on the slide glass carrying disc.
Has the advantages that: set the slide tray into single slide tray, thereby make each slide along fore-and-aft direction single row arrangement on the slide tray, the auxiliary reagent liquid flow car that the back-and-forth movement set up like this, just can satisfy the demand that corresponds the interpolation auxiliary reagent of the slide in hatching the storehouse through the back-and-forth movement, do not need auxiliary reagent liquid flow car to move about, need not set up the guide rail that supplies auxiliary reagent liquid flow car to move about in the frame, be favorable to simplifying the structure, and simultaneously, auxiliary reagent liquid flow car need not move about, can reduce occupation space, compact structure, be favorable to reducing the equipment volume.
Drawings
FIG. 1 is a schematic view showing the overall structure of an automatic immunohistochemical staining machine in example 1 of the present invention;
FIG. 2 is a schematic view of the internal structure of FIG. 1 with the top plate, the left outer plate and the front side plate of the box body removed;
FIG. 3 is a schematic view of the robotic arm of FIG. 2 and a mounting structure on the frame;
FIG. 4 is a schematic view of the mounting structure of the auxiliary reagent flow cart in FIG. 2 on a rack;
FIG. 5 is a schematic view of the auxiliary reagent flow cart of FIG. 4 with the housing removed;
FIG. 6 is a first schematic structural view of a reagent cartridge;
fig. 7 is a schematic structural diagram of the reagent bin.
In the figure: 1. a frame; 11. a left outer plate of the box body; 12. a box top plate; 13. a sample processing chamber front side plate; 14. a cover plate for the inlet and outlet of the slide glass carrying tray; 15. a sample processing chamber left side panel; 16. a front-side mechanical arm moving guide rail; 17. a rear-side mechanical arm moving guide rail; 18. the liquid flow vehicle moves the guide rail; 19. a front cover plate of the auxiliary reagent supply chamber; 110. a driving belt wheel of the liquid flow vehicle; 111. a liquid-flow vehicle drive belt; 1011. a first robot arm; 1012. a second mechanical arm; 1021. a first incubation bin; 1022. a second incubation bin; 1023. a third incubation bin; 1024. a fourth incubation bin; 1031. a first fluid flow vehicle; 1032. a second fluid vehicle; 1033. a third fluid flow vehicle; 1034. a fourth fluid flow vehicle; 1041. a reagent bin; 1051. carrying a slide tray; 1061. a first heating constant temperature module; 1062. a second heating constant temperature module; 1071. an injection pump; 1081. an auxiliary reagent tank; 1091. heating the constant-temperature air inlet hole; 1101. an inlet and an outlet of the reagent bin; 1102. an auxiliary reagent bin; 21. an arm body; 22. a front mechanical arm moving seat; 23. a rear mechanical arm moving seat; 24. a sample adding moving seat; 25. a sample adding needle; 31. a cover plate; 311. avoiding the mouth; 41. a refrigeration module; 42. a kit; 51. a lifting seat; 52. a lifting drive motor; 53. and (4) filling a needle with an auxiliary reagent.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
It is noted that relational terms such as "first" and "second," and the like, which may be present in embodiments of the present invention, are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, terms such as "comprises," "comprising," or any other variation thereof, which may be present, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the recitation of "comprising an … …" or the like that may occur does not exclude the presence of additional like elements in a process, method, article, or apparatus that comprises the element.
In the description of the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" when they are used are to be construed broadly, e.g., as meaning a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; either directly or indirectly through intervening media, or may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those skilled in the art from specific situations.
In the description of the present invention, unless otherwise specifically stated or limited, the term "provided" may be used in a broad sense, for example, the object of "provided" may be a part of the body, or may be arranged separately from the body and connected to the body, and the connection may be detachable or non-detachable. The specific meaning of the above terms in the present invention can be understood by those skilled in the art from specific situations.
The present invention will be described in further detail with reference to examples.
Example 1 of the automatic immunohistochemical staining machine of the present invention:
as shown in fig. 1 and 2, the automated immunohistochemical dyeing machine includes a frame 1, and the frame 1 is a rectangular box including a frame body and each box plate fixed to the frame body, and each box plate forms a closed space in the frame 1. There are upper and lower two-layer bin in the frame 1, and the upper strata bin is the sample processing bin, is equipped with arm, reagent storehouse 1041 in the sample processing bin, incubates storehouse, auxiliary reagent liquid stream car to carry out dyeing treatment to the tissue sample on the slide, and the lower floor bin is auxiliary reagent supply bin, is provided with auxiliary reagent jar 1081 and syringe pump 1071 in it in order to supply auxiliary reagent to auxiliary reagent liquid stream car and arm.
The box board on the frame 1 comprises a box left outer board 11, a box right outer board, a box bottom board, a box top board 12, a box back board and a box front door, the sample processing chamber is provided with a sample processing chamber left side board 15 and a sample processing chamber right side board, and the sample processing chamber left side board 15 and the sample processing chamber right side board are respectively arranged on the inner sides of the box left outer board 11 and the box right outer board at intervals.
The number of the incubation bins is four, the incubation bins are respectively a first incubation bin 1021, a second incubation bin 1022, a third incubation bin 1023 and a fourth incubation bin 1024, each incubation bin is independently arranged, a slide glass carrying tray 1051 is arranged in each incubation bin, each slide glass carrying tray 1051 is a single-row slide glass carrying tray, slide glass mounting positions on each single-row slide glass carrying tray are arranged in the front-back direction, and therefore slides are arranged in a row in the front-back direction after each slide glass is placed on the slide glass carrying tray. The slide glass tray 1051 is provided with 15 slide glass mounting positions, so that each incubation bin has 15 independent slide glass positions, and the total number of the 4 groups of the slide glass positions is 60, thereby greatly improving the dyeing flux.
Four are hatched the storehouse and are arranged in proper order in sample treatment warehouse bottom along left right direction, the glass slide year dish 1051 that carries the slide is placed in hatching the storehouse, first hatch storehouse 1021, storehouse 1022 is hatched to the second and is hatched a storehouse front bezel of hatching in common, storehouse 1023 is hatched to the third, storehouse 1024 is hatched in the fourth and is hatched a storehouse front bezel of hatching in common, it supplies the slide to carry the glass slide to carry dish 1051 to pass in and out to be equipped with on the storehouse front bezel to hatch, the front side of storehouse front bezel is equipped with slide and carries a dish business turn over mouth apron 14 respectively, slide carries a dish business turn over mouth apron 14 and articulates on the frame body of frame 1 and can open and close. A sample processing chamber front side plate 13 is arranged above the slide glass carrying plate inlet and outlet cover plate 14, and the sample processing chamber front side plate 13 is fixed on the frame body and seals the upper spaces of the reagent chamber 1041 and each incubation chamber.
The front opening of the auxiliary reagent supply chamber at the lower layer of the frame 1 is provided with an openable auxiliary reagent supply chamber front cover plate 19. The front side plate 13 of the sample processing chamber, the cover plate 14 of the slide tray inlet and outlet, and the front cover plate 19 of the auxiliary reagent supply chamber together form the front door of the box body of the frame 1.
The sample processing chamber left side plate 15, sample processing chamber right side plate are equipped with first heating constant temperature module 1061, second heating constant temperature module 1062 respectively on, and the heating constant temperature module includes electric heating element and sends the fan in the sample processing chamber with electric heating element production heat. Heating constant temperature air inlets 1091 are arranged on the left outer plate 11 and the right outer plate of the box body at positions corresponding to the heating constant temperature modules. Even in cold winter or in cold regions, the temperature around the 4 incubation bins can be controlled to be 20-30 ℃, the activity of the reagent is ensured, and the dyeing quality of the reagent is not influenced by the external environment temperature.
As shown in fig. 2 and 3, a front arm moving guide 16 and a rear arm moving guide 17 are provided on the top of the frame body of the rack 1, two arms are provided, which are a first arm 1011 and a second arm 1012, respectively, the two arms are arranged side by side in the left-right direction, the arm includes an arm body 21 extending in the front-rear direction, a arm moving base is provided on the arm body 21, the arm moving base includes a front arm moving base 22 and a rear arm moving base 23 provided at both ends of the arm body 21, respectively, the front arm moving base 22 is mounted on the front arm moving guide 16, the rear arm moving base 23 is mounted on the rear arm moving guide 17, so that the arms are arranged on the rack 1 in the left-right direction movement, the rack 1 is provided with a arm driving mechanism for driving the rear arm moving base 23 to move left and right on the arm moving guide, each arm is operated independently, do not interfere with each other. The mechanical arm is provided with the test distance sensor, so that the collision condition of the two mechanical arms can be avoided. The front arm movement guide 16 and the rear arm movement guide 17 are common to both arms. The guide rails at the two ends of the mechanical arm bear the load of the mechanical arm together, so that the reliability and the stability of the operation of the mechanical arm are improved.
The arm body 21 of arm goes up the back and forth direction and removes and dispose application of sample removal seat 24, reagent adds and is equipped with application of sample needle 25 on the removal seat, application of sample needle 25 reciprocates the configuration and removes on application of sample removal seat 24, be provided with the application of sample needle actuating mechanism that drive application of sample needle 25 reciprocated on the arm body 21, application of sample needle actuating mechanism includes the transfer line that extends about and installs the driving motor at arm body 21 rear end, the dead rotation disposes the gear on the transfer line, the gear is the configuration of sliding from beginning to end on the transfer line, and be equipped with the limiting plate that carries out front and back spacing to the gear on application of sample removal seat 24 in order to drive the gear and slide on the transfer line when application of sample removes seat 24 back-and-forth movement, be provided with the rack with gear engagement on application of sample needle 25, driving motor drives the gear rotation through the drive transfer line, and then drive application of sample needle 25 up-and-down motion.
As shown in fig. 2, 4 and 5, the rack 1 is provided with four auxiliary reagent flow carts, which are respectively a first flow cart 1031, a second flow cart 1032, a third flow cart 1033 and a fourth flow cart 1034, each auxiliary reagent flow cart corresponds to one incubation bin, so that each incubation bin is correspondingly provided with an auxiliary reagent flow cart, and the auxiliary reagent flow carts are located on the right side of the corresponding incubation bin. The auxiliary reagent liquid flow vehicle comprises a liquid flow vehicle moving seat which is arranged on the frame 1 in a back-and-forth moving mode, a cover is arranged on the liquid flow vehicle moving seat, the liquid flow vehicle moving seat comprises a seat body and a lifting seat 51 arranged on the seat body, a lifting driving motor 52 for driving the lifting seat 51 to lift is arranged on the seat body of the liquid flow vehicle moving seat, and an auxiliary reagent filling needle 53 for adding an auxiliary reagent to a slide is arranged on the lifting seat 51.
The cover plate 31 is arranged on the incubation bin, the avoidance ports 311 which are in one-to-one correspondence with the slide glass are arranged on the cover plate 31, and when the auxiliary reagent needs to be filled, the auxiliary reagent filling needle 53 can be lowered to penetrate through the avoidance ports 311 to be close to the slide glass, so that the auxiliary reagent is accurately filled on the slide glass, and splashing is avoided.
The rack 1 is provided with a liquid flow vehicle moving guide rail 18, a base body of the liquid flow vehicle moving base is arranged on the liquid flow vehicle moving guide rail 18, the rack 1 is provided with a liquid flow vehicle moving base driving mechanism for driving the liquid flow vehicle moving base to move back and forth on the liquid flow vehicle moving guide rail 18, the liquid flow vehicle moving base driving mechanism comprises a liquid flow vehicle driving motor arranged at the rear end of the liquid flow vehicle moving guide rail 18 and a liquid flow vehicle driving belt wheel 110 in transmission connection with the motor, a liquid flow vehicle driving belt 111 is arranged on the liquid flow vehicle driving belt wheel 110, and the liquid flow vehicle driving belt 111 is fixed with the base body of the liquid flow vehicle moving base to drive an auxiliary reagent liquid flow vehicle to move back and forth, so that auxiliary reagents are added to slides which are arranged in a row in the front-back direction.
As shown in fig. 2, 6 and 7, the reagent bin 1041 is of a drawer-type structure, and includes a reagent box frame capable of being drawn out, reagent slots for accommodating the reagent box 42 are provided on the reagent box frame, reagents are stored in the reagent box 42, 5 rows of reagent slots are provided in the reagent bin 1041, each row of reagent slots has 14 reagent slots, 70 reagent slots are provided, and the reagent bin has a large capacity, so that the simultaneous staining of various tissues can be satisfied. The reagent magazine 1041 has an upper cover plate 31, and the upper cover plate 31 is provided with perforations corresponding to the respective placement grooves, so that the sample injection needle 25 can enter the reagent cartridge 42 to aspirate the reagent. The bottom of the reagent bin 1041 is provided with a high-power high-efficiency refrigeration module 41, so that the inside of the whole reagent bin 1041 is in a low-temperature state for a long time, the reagent is kept fresh for a long time and is not deteriorated, and the dyeing effect is ensured.
As shown in fig. 2, the auxiliary reagent supply chamber at the lower layer of the rack 1 is divided into an upper space and a lower space, the lower space is an auxiliary reagent chamber 1102 for accommodating each auxiliary reagent tank 1081, and the auxiliary reagent tank 1081 contains an auxiliary reagent, which is a cleaning solution in this embodiment. The lower layer space in the auxiliary reagent supply cabin is an injection pump cabin, a high-precision injection pump 1071 is placed in the injection pump cabin, the auxiliary reagent can be pumped accurately and quantitatively, an auxiliary reagent tank 1081 is connected with the injection pump 1071 through a pipeline, the auxiliary reagent tank 1081 and the injection pump 1071 form an auxiliary reagent pumping device, the injection pump 1071 has six pumps, two pumps respectively feed liquid quantitatively to two mechanical arms, the other four pumps feed liquid quantitatively to four liquid flow vehicles, an auxiliary reagent feeding needle 53 is connected with the corresponding injection pump 1071 through a pipeline, a sample feeding needle 25 on the mechanical arm is also connected with the corresponding injection pump 1071 through a pipeline, and the two mechanical arms and the four liquid flow vehicles can be ensured to run completely and independently, so that the sample feeding needle 25 on the mechanical arm not only can add the reagent in the reagent cabin 1041 onto the slide, but also can add the auxiliary reagent onto the slide through the auxiliary reagent pumping device, the operation action of the mechanical arm is convenient to allocate, the mechanical arm is beneficial to being fully utilized, and the waiting time is shortened.
When the device is used, a slide with a tissue sample is placed on the slide tray 1051, the slide tray 1051 is placed in an incubation bin, the mechanical arms act, a reagent is sucked from the reagent bin 1041 through the sample adding needle 25 and is dripped on the slide, and the two mechanical arms work simultaneously to sequentially add samples to the slides in the incubation bins; when the slide needs to be cleaned, the auxiliary reagent liquid flow cart acts to add cleaning liquid on the slide through the auxiliary reagent adding needle 25 for cleaning, and each auxiliary reagent liquid flow cart independently acts respectively to add cleaning liquid to the slide in each incubation bin.
Through the auxiliary reagent liquid flow car of configuration to each hatching storehouse, be exclusively used in and add auxiliary reagent, the arm need not to go to add auxiliary reagent again like this, and the arm can independent operation separately with auxiliary reagent liquid flow car, and different steps can go on in step in the dyeing process, can significantly reduce the arm used repeatedly and the latency who produces like this, and to a great extent has shortened total operating time, improves the treatment effeciency. And, through setting the slide tray into single slide tray, thereby make each slide along fore-and-aft direction single row arrangement on the slide tray, the auxiliary reagent liquid flow car that the back-and-forth movement set up like this, just can satisfy the demand that corresponds the supplementary reagent of the slide in the incubation storehouse through the back-and-forth movement, do not need the auxiliary reagent liquid flow car to remove about, need not set up the guide rail that supplies the auxiliary reagent liquid flow car to remove about in the frame, be favorable to simplifying the structure, and simultaneously, the auxiliary reagent liquid flow car need not remove about, can reduce occupation space, moreover, the steam generator is compact in structure, be favorable to reducing the equipment volume.
Example 2 of the automated immunohistochemical staining machine of the present invention:
this example differs from example 1 in that the auxiliary reagent flow cart in example 1 is located to the right of the corresponding incubation well. In this embodiment, the auxiliary reagent flow cart is disposed on the left side of the corresponding incubation bin.
Example 3 of the automated immunohistochemical staining machine of the present invention:
the difference between the embodiment and embodiment 1 is that in embodiment 1, the driving motor of the liquid flow cart drives the driving pulley of the liquid flow cart, and the driving pulley of the liquid flow cart drives the auxiliary reagent liquid flow cart to move through the transmission of the liquid flow cart. In the embodiment, the screw rod nut mechanism is used for transmission, the liquid flow moving seat is screwed on the screw rod, so that the motor drives the screw rod to rotate, and the auxiliary reagent liquid flow vehicle is driven to move.
Example 4 of the automated immunohistochemical staining machine of the present invention:
this embodiment is different from embodiment 1 in that the auxiliary reagent filling needle in embodiment 1 is used for adding a cleaning reagent. In this embodiment, the auxiliary reagent filling needle is used for filling a repair reagent or a dewaxing reagent, and an auxiliary reagent tank containing a corresponding reagent is placed in the auxiliary reagent chamber.
Example 5 of the automated immunohistochemical staining machine of the present invention:
this example is different from example 1 in that four incubation chambers are provided in example 1. In this embodiment, the number of the incubation chambers is two. In other embodiments, the incubation bins can be arranged in three or more than five.
Example 6 of the automated immunohistochemical staining machine of the present invention:
this example differs from example 1 in that there are two robotic arms in example 1, and two incubation chambers share a single robotic arm. In the embodiment, the incubation bins are arranged into six incubation bins, and the three incubation bins share one mechanical arm. In other embodiments, the number of the incubation bins may be two, and the mechanical arm is only provided corresponding to the two incubation bins.
Example 7 of the automated immunohistochemical staining machine of the present invention:
the present embodiment is different from embodiment 1 in that the two robot arms in embodiment 1 share the robot arm movement guide. In this embodiment, the two robot arms do not share the robot arm moving guide rail, and the rack is respectively provided with the first robot arm moving guide rail and the second robot arm moving guide rail which are independent, so that the two robot arms can be respectively moved and assembled.
Example 8 of the automated immunohistochemical staining machine of the present invention:
the present embodiment is different from embodiment 1 in that the robot arm in embodiment 1 is mounted on the frame so that both front and rear ends thereof are guided to move left and right. In the present embodiment, only the rear end of the robot arm is mounted on the frame so as to be movable in the left-right direction.
Finally, it should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and not intended to limit the present invention, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and equivalents can be made in the technical solutions described in the foregoing embodiments without inventive effort, or some technical features of the present invention may be substituted with equivalents. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. An automatic immunohistochemical dyeing machine comprises a rack, wherein a mechanical arm is arranged on the rack, the mechanical arm extends in the front-back direction and is movably arranged on the rack in the left-right direction, a reagent bin for storing a reagent box and an incubation bin for placing a slide are arranged on the rack, and the mechanical arm comprises a sample adding needle for adding a reagent in the reagent bin onto the slide; an auxiliary reagent filling needle is arranged on the liquid flow vehicle moving seat and connected with an auxiliary reagent pumping device arranged on the rack; the frame is provided with a liquid flow vehicle moving seat driving mechanism for driving the liquid flow vehicle moving seat to move back and forth; incubate the storehouse and be provided with two at least along left right direction, each is incubated the storehouse and all is correspondingly furnished with auxiliary reagent liquid flow car, and two at least arms of incubating the storehouse sharing are hatched to the storehouse.
2. The automated immunohistochemical staining machine of claim 1, wherein at least two robotic arms are arranged side-to-side, the reagent chamber is located between two adjacent robotic arms, and each incubation chamber sharing one robotic arm is located on the same side of the reagent chamber.
3. The automated immunohistochemical dyeing machine according to claim 2, wherein the frame is provided with a robot arm moving guide rail extending in the left-right direction, the robot arm comprises a robot arm moving base mounted on the robot arm moving guide rail, the frame is provided with a robot arm driving mechanism for driving the robot arm moving base to move left and right on the robot arm moving guide rail, and the robot arms share the robot arm moving guide rail.
4. The automated immunohistochemical staining machine of claim 1 wherein the robotic arm is movably mounted to the frame at both the front and rear ends thereof in a left-right guided manner.
5. The automated immunohistochemical staining machine of any one of claims 1 to 4, wherein the moving base of the liquid flow carriage comprises a base body and a lifting base disposed on the base body, the auxiliary reagent filling needle being disposed on the lifting base.
6. The automated immunohistochemical staining machine of any one of claims 1-4 wherein the auxiliary reagent pumping device is further connected to a sample application needle on the robotic arm.
7. An automatic immunohistochemical staining machine according to any one of claims 1 to 4 wherein the bottom of the reagent chamber is provided with a refrigeration module.
8. The automated immunohistochemical staining machine of any one of claims 1 to 4, wherein the rack is provided with a layered sample processing chamber and an auxiliary reagent supply chamber at a lower side of the sample processing chamber, the mechanical arm, the reagent chamber and the incubation chamber are disposed in the sample processing chamber, and the auxiliary reagent pumping means is disposed in the auxiliary reagent supply chamber.
9. The automated immunohistochemical staining machine of any one of claims 1 to 4 wherein the rack is provided with a sample treatment chamber, the sample treatment chamber is provided with a heating thermostat module for maintaining the temperature of the sample treatment chamber, the heating thermostat module comprises an electric heating element and a fan for supplying heat generated by the electric heating element to the sample treatment chamber.
10. The automated immunohistochemical staining machine of any one of claims 1 to 4, wherein the incubation chamber has a slide tray, the slide tray being a single row slide tray, the slide mounting locations on the single row slide tray being arranged in a front-to-back direction for aligning the slides in the front-to-back direction after the slides are placed on the slide tray.
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| CN202111667389.1A CN114152496A (en) | 2021-12-31 | 2021-12-31 | Automatic immunohistochemical dyeing machine |
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Cited By (1)
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| CN116793796A (en) * | 2022-11-07 | 2023-09-22 | 广州泰立生物科技有限公司 | Automatic immunohistochemical dyeing machine and immunohistochemical dyeing process |
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