CN110514500A - A kind of high throughput automatic sample pre-treatment instrument, application and application method - Google Patents
A kind of high throughput automatic sample pre-treatment instrument, application and application method Download PDFInfo
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- CN110514500A CN110514500A CN201910903132.8A CN201910903132A CN110514500A CN 110514500 A CN110514500 A CN 110514500A CN 201910903132 A CN201910903132 A CN 201910903132A CN 110514500 A CN110514500 A CN 110514500A
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- 238000002203 pretreatment Methods 0.000 title claims abstract description 29
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- 238000009434 installation Methods 0.000 description 1
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 1
- 229940107698 malachite green Drugs 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4022—Concentrating samples by thermal techniques; Phase changes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4022—Concentrating samples by thermal techniques; Phase changes
- G01N2001/4027—Concentrating samples by thermal techniques; Phase changes evaporation leaving a concentrated sample
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention discloses a kind of high-throughput automatic sample pre-treatment instrument, application and application method, its main feature is that XYZ shaft mechanical mould group is arranged on pedestal is arranged mechanical agitator, automatic liquid absorbing arm and automatic liquid-feeding arm；Devil liquor recovery slot is set on pedestal, metal incubation slot is set above devil liquor recovery slot, sample extraction tank is set in metal incubation slot；Solid Phase Extraction pipe holder is set side by side with metal incubation slot, and solid phase extraction tube is arranged in Solid Phase Extraction pipe holder；Imbibition head bracket is set to metal incubation slot side, the disposable fluid suction head of setting in imbibition head bracket；Liquid concentrator is movably arranged between Solid Phase Extraction pipe holder and devil liquor recovery slot by track；Electromagnetism fluid filling pump is set on the base and connect with automatic liquid-feeding arm.The beneficial effects of the invention are as follows the cooperatings for passing through sample extraction tank, solid phase extraction tube, liquid concentrator, avoid sample cross contamination problem, while carrying out the processing of 12 samples, it can be achieved that whole process is unattended, and working efficiency greatly improves.
Description
Technical field
The invention belongs to technical field of food detection more particularly to a kind of high-throughput automatic sample pre-treatment instrument, application
And application method, it is identified for food safety detection, forensic, the sample pre-treatments of toxicological analysis.
Background technique
Sample pre-treatments are usually the first step analyzed, and the result of the step will affect subsequent analytic process.In food
Safety detection, forensic identification, toxicological analysis field, are presently mainly carried out at sample by manual control device
Reason will use a large amount of organic and inorganic reagent and need the cooperation of a variety of pre-treatment instruments in carrying out the course of work,
Large labor intensity has an impact to the health of operator, and especially processing has a peculiar smell, when corrupt sample, to operator's
Basic quality requirement is very high, careless slightly just to will appear operating error, leads to the mistake of testing result.
Summary of the invention
1. technical problems to be solved
Matching using a large amount of organic and inorganic reagent and a variety of pre-treatment instruments when for sample pre-treatments in the prior art
It closes, large labor intensity has an impact to the health of operator, and especially processing has a peculiar smell, when corrupt sample, to operator
Basic quality require it is very high, the defects of being easy to appear operating error, lead to testing result mistake, it is an object of the invention to mention
For a kind of high-throughput automatic sample pre-treatment instrument, application and application method, it is greatly improved the extraction working efficiency of sample, especially
It has peculiar smell for processing, and corrupt sample is, it can be achieved that whole unattended.
2. technical solution
To achieve the above object, the present invention adopts the following technical scheme:
A kind of high throughput automatic sample pre-treatment instrument, its main feature is that including pedestal, XYZ shaft mechanical mould group, metal incubation
Slot, sample extraction tank, Solid Phase Extraction pipe holder, imbibition head bracket, liquid concentrator, devil liquor recovery slot, electromagnetism fluid filling pump and work
Control machine；XYZ shaft mechanical mould group is set on the pedestal, and the XYZ shaft mechanical mould group includes X-axis, Y-axis and Z axis, the X-axis water
Flat to be arranged and connect with pedestal, Y-axis is vertically arranged and is slidably connected with X-axis, and the Z axis and X-axis are horizontally disposed simultaneously in 90 ° of angles
It is slidably connected with Y-axis；Mechanical agitator, automatic liquid absorbing arm and automatic liquid-feeding arm are set on the Z axis；The bottom of the X-direction
Devil liquor recovery slot is set on seat, metal incubation slot is set above the devil liquor recovery slot, the sample extraction tank is incubated set on metal
It educates in slot；The Solid Phase Extraction pipe holder is set side by side with metal incubation slot, and solid phase extraction tube is arranged in Solid Phase Extraction pipe holder；
The imbibition head bracket is set to metal incubation slot side, the disposable fluid suction head of setting in imbibition head bracket；The liquid concentration
Device is movably arranged between Solid Phase Extraction pipe holder and devil liquor recovery slot by track；The electromagnetism fluid filling pump is set on the base simultaneously
It is connect with automatic liquid-feeding arm, the medium of electromagnetism fluid filling pump conveying is sample extracting solution, activating solution, scavenging solution；The XYZ shaft mechanical
Mould group, mechanical agitator, electromagnetism fluid filling pump, automatic liquid-feeding arm, automatic liquid absorbing arm and industrial personal computer are electrically connected, as built in industrial personal computer
Driver control.
Further, the X-axis of the XYZ shaft mechanical mould group includes the first X-axis and the second X-axis being parallel to each other, Y-axis packet
The first Y-axis and the second Y-axis being parallel to each other are included, Z axis includes the first Z axis and the second Z axis being parallel to each other；The first X-axis water
Flat to be arranged and connect with pedestal, the first Y-axis is vertically arranged and is slidably connected with the first X-axis, and first Z axis is in the first X-axis
90 ° of angles are horizontally disposed and are slidably connected with the first Y-axis；Second Z axis is slidably connected with the second X-axis, second Y-axis with
Second Z axis is slidably connected；Second Y-axis is connect with the first Z axis by connecting plate, and mechanical stirring is arranged below the connecting plate
Device；The automatic liquid-feeding arm and automatic liquid absorbing arm being slidably connected are set on first Z axis.
Further, the sample extraction tank includes tank body, setting is separated by filtration membrane cartridge in the tank body the
Upper cover plate is arranged in the top of one cavity and the second cavity, the tank body；Filter membrane is set on the filtration membrane cartridge；The upper cover
Plate is located at the setting of the side above the first cavity transmission shaft, the connection insertion intracorporal stirring spiral shell of the first chamber below the transmission shaft
Bar, the connector that the setting of transmission shaft top is connect with blender；The upper cover plate is located at the setting of the side above the second cavity and adds
Fluid apertures；The tank body is made using the plastic material that resistance to organic reagent corrodes；The plastic material of the resistance to organic reagent corrosion is
PP polypropylene, PE polyethylene；The volume of tank body is 100cm3；The first cavity of several connections and the second chamber are set on filtration membrane cartridge
Filter membrane is arranged on the through-hole in the through-hole of body；The material selection of filter membrane but it is not limited to nylon, cellulose acetate, polypropylene
Grid, glass fibre, polytetrafluoroethylene (PTFE), nitrocellulose, polyamide.In use, upper cover plate is opened, the sample that needs are extracted
It being put into the first cavity, covers upper cover plate, sample extracting solution is added in the second cavity by liquid-adding device by liquid filling hole, due to
The osmosis of filter membrane, sample extracting solution penetrate into the first cavity, carry out sample incubation；Blender is inserted into connector
Interior, blender rotation drives transmission shaft and is located at the intracorporal agitating screw rotation of the first chamber；Make the intracorporal sample of the first chamber and sample
Product extracting solution is sufficiently mixed, and the active principle in sample is discharged into sample extracting solution；Sample extraction dissolved with active principle
Liquid is penetrated into the second cavity by filter membrane, can guarantee to obtain in the second cavity so clear dissolved with active principle
Sample extracting solution, muddy sample mixture stay in the first cavity；Liquid absorption device, which protrudes into liquid filling hole, will be dissolved with active principle
Sample extracting solution draw move into solid phase extraction tube in handled.
Further, the solid phase extraction tube is formed by the plastic material that resistance to organic reagent corrodes by mold injection,
Volume is 6mL, plastic material PP polypropylene, PE polyethylene that resistance to organic reagent corrodes etc.；The solid phase extraction tube inner stuffing
Powder can select and be not limited to HLB hydrophilic-lipophilic balance resin, MCX mixed type cation exchange resin, MAX mixed type yin from
Sub-exchange resin, WCX weak cation exchange resin, WAX weak anion exchange resin, C18 block octadecyl resin, C8 octyl
Resin, Carb-GCB Graphon, Flosisil Fo Luoli tripoli, ALA aluminium oxide, ALN aluminium oxide, ALB aluminium oxide, PSA
Ethylenediamine-N- propyl resin is single or is used in mixed way.
Further, setting liquid concentration pipe and heating device in the liquid concentrator, in the liquid concentration pipe
Hot wind syringe needle is set；The head bending of the hot wind syringe needle, syringe needle hole is opposite with liquid concentration tube wall, so that hot wind is being blown to
On tube wall, liquid splash, sample cross contamination caused by air-flow when avoiding extract liquor；The heating device and hot wind syringe needle and work
Control machine is electrically connected, and industrial personal computer controls heating device and heats up to liquid concentration pipe, while the hot wind needle blow built in liquid concentration pipe
Hot wind out carries out interior external heat to liquid concentration pipe, and liquid volatilizes in liquid concentration pipe after a period of time, liquid concentrator drop
Temperature, hot wind syringe needle stop blowout hot wind.
Further, 12 hole locations are set on the metal incubation slot, for placing 12 sample extraction tanks；It is described solid
Solid phase extraction tube quantity built in phase extracting tube is 12, and liquid concentration pipe quantity is 12, and the hot wind syringe needle quantity is 12
It is a, it is handled while meeting 12 samples.
Further, the high-throughput automatic sample pre-treatment instrument also sets up shell, and the shell is connect with pedestal,
Air inlet fan is set on the shell and air ejector fan, sample place window, the industrial personal computer is arranged on the shell, the air inlet wind
Fan and air ejector fan and industrial personal computer are electrically connected.
The present invention also provides above-mentioned high-throughput automatic sample pre-treatment instrument extract food or raw-food material or in
The application in pesticide, additive, illegal additive, coloring agent, weighting agent, clenbuterol hydrochloride, drug, bacterium in medicinal material.
A kind of application method of high throughput automatic sample pre-treatment instrument, its main feature is that the following steps are included:
S1, sample liquid feeding, are controlled by industrial personal computer, are driven by electromagnetism fluid filling pump, by automatic liquid-feeding arm by sample extraction
Liquid is added in sample extraction tank；
S2, sample incubation, are controlled by industrial personal computer, the heating of metal incubation slot, the solidliquid mixture liter in sample extraction tank
After a certain period of time, active principle in sample is sufficiently discharged into extracting solution for temperature；
S3, sample stirring, are controlled by industrial personal computer, drive XYZ shaft mechanical mould group, the stirring-head of mechanical agitator is inserted into
Into sample extraction tank connecting shaft, agitating screw starts high-speed rotation, enables solid and liquid in sample extraction tank sufficient
Mixing, active principle in sample is sufficiently discharged into sample extracting solution；
S4, sample filtering are completed in sample extraction tank, and clear sample extracting solution can be by installing in sample extraction tank
Sample extraction tank filter membrane, be muddy solidliquid mixture in the first cavity, for dissolved with active principle in the second cavity
Sample extracting solution；
S5, solid-phase extraction column activation, are controlled by industrial personal computer, drive XYZ shaft mechanical mould group, driven by electromagnetism fluid filling pump,
Activating solution is added in solid phase extraction tube by automatic liquid-feeding arm, the liquid of outflow is directly entered devil liquor recovery slot；
S6, sample liquid loading, are controlled by industrial personal computer, drive XYZ shaft mechanical mould group, automatic liquid absorbing arm will be in the second cavity
Sample extracting solution absorption dissolved with active principle is added in solid phase extraction tube, and the liquid of outflow is directly entered devil liquor recovery
Slot；
S7, Solid Phase Extraction column purification, are controlled by industrial personal computer, are driven XYZ shaft mechanical mould group, are driven by electromagnetism fluid filling pump,
Scavenging solution is added in solid phase extraction tube by automatic liquid-feeding arm, the liquid of outflow is directly entered devil liquor recovery slot；
S8, sample liquid recycling, are controlled by industrial personal computer, drive XYZ shaft mechanical mould group, driven, passed through by electromagnetism fluid filling pump
Scavenging solution is added in solid phase extraction tube by automatic liquid-feeding arm, and the liquid of outflow enters in liquid concentrator pipe；
S9, sample liquid concentration, are controlled by industrial personal computer, liquid concentrator heating, while the hot wind built in liquid concentrator
Syringe needle blows out hot wind, carries out interior external heat to the concentration tube in liquid concentrator, liquid volatilizes in concentration tube after a period of time, liquid
The cooling of body inspissator, hot wind syringe needle stop blowout hot wind.
3. beneficial effect
Compared with prior art, the beneficial effects of the present invention are: passing through sample extraction tank, solid phase extraction tube, liquid concentration
The cooperating of device avoids sample cross contamination problem, and sample extraction tank effective component extraction efficiency is high, carries out 12 samples simultaneously
This processing, has peculiar smell for processing, and corrupt sample greatly improves work efficiency, it can be achieved that whole unattended.
Detailed description of the invention
It, below will be to specific reality in order to illustrate more clearly of invention specific embodiment or technical solution in the prior art
Apply mode or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, be described below in it is attached
Figure is some embodiments of invention, for those of ordinary skill in the art, without creative efforts,
It is also possible to obtain other drawings based on these drawings.
Fig. 1 is the right view three-dimensional appearance schematic diagram of the present invention.
Fig. 2 is left view three-dimensional appearance schematic diagram of the present invention.
Fig. 3 is sample extraction jar structure schematic diagram of the present invention.
Fig. 4 is upper cover plate structural schematic diagram of the present invention.
Fig. 5 is tank structure schematic diagram of the present invention.
Fig. 6 is schematic diagram of internal structure of the present invention (one).
Fig. 7 is schematic diagram of internal structure of the present invention (two).
Fig. 8 is schematic perspective view (three) inside the present invention.
Fig. 9 is fluid present invention inspissator structural schematic diagram.
In figure: 1- industrial personal computer 2- air ejector fan 3- air inlet fan 4- sample places window 5- tank body 6- filtration membrane cartridge
7- the first cavity 8- the second cavity 9- upper cover plate 10- through-hole 11-XYZ shaft mechanical mould group 12- metal incubation slot 13- transmission
The disposable fluid suction head 18- liquid concentrator of axis 14- electromagnetism fluid filling pump 15- mechanical agitator 16- solid phase extraction tube 17-
The automatic liquid absorbing arm 21- automatic liquid-feeding arm 22- hot wind syringe needle 23- agitating screw 24- connector of 19- devil liquor recovery slot 20-
25- liquid filling hole 26- the first X-axis 27- the first Y-axis 28- the first Z axis 29- the second X-axis 30- the second Y-axis the second Z axis of 31-
32- shell 33- pedestal.
Specific embodiment
It is clearly and completely described below in conjunction with technical solution of the attached drawing to invention, it is clear that described embodiment
It is invention a part of the embodiment, instead of all the embodiments.Based on the embodiment in invention, those of ordinary skill in the art exist
Every other embodiment obtained under the premise of creative work is not made, the range of invention protection is belonged to.
In the description of invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical", " water
It is flat ", "inner", the instructions such as "outside" orientation or positional relationship be to be based on the orientation or positional relationship shown in the drawings, merely to just
In description invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation, with spy
Fixed orientation construction and operation, therefore should not be understood as the limitation to invention.
In the description of invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, it can understand that above-mentioned term is being sent out with concrete condition
Concrete meaning in bright.
As shown in Figures 1 to 9, a kind of high-throughput automatic sample pre-treatment instrument, its main feature is that including pedestal 33, XYZ axis
Mechanical mould group 11, sample extraction tank, Solid Phase Extraction pipe holder, imbibition head bracket, liquid concentrator 18, is given up at metal incubation slot 12
Liquid accumulator tank 19, electromagnetism fluid filling pump 14 and industrial personal computer 1；XYZ shaft mechanical mould group 11, XYZ shaft mechanical mould group 11 are set on pedestal 33
Include X-axis, Y-axis and Z axis, X-axis is horizontally disposed and connect with pedestal 33, and Y-axis is vertically arranged and is slidably connected with X-axis, Z axis and
X-axis is horizontally disposed in 90 ° of angles and is slidably connected with Y-axis；Mechanical agitator 15, automatic liquid absorbing arm 20 and automatic are set on Z axis
Liquid adding arm 21；Devil liquor recovery slot 19 is set on the pedestal 33 of X-direction, metal incubation slot 12 is set above devil liquor recovery slot 19,
Sample extraction tank is set in metal incubation slot 12；Solid Phase Extraction pipe holder is set side by side with metal incubation slot 12, solid phase extraction tube
Solid phase extraction tube 16 is set in bracket；Imbibition head bracket is set to 12 side of metal incubation slot, and setting is primary in imbibition head bracket
Property fluid suction head 17；Liquid concentrator 18 is movably arranged between Solid Phase Extraction pipe holder and devil liquor recovery slot 19 by track；Electromagnetism
Fluid filling pump 14 is arranged on pedestal 33 and connect with automatic liquid-feeding arm 21, and the medium that electromagnetism fluid filling pump 14 conveys is sample extraction
Liquid, activating solution, scavenging solution；It is XYZ shaft mechanical mould group 11, mechanical agitator 15, electromagnetism fluid filling pump 14, automatic liquid-feeding arm 21, automatic
Liquid absorbing arm 20 and industrial personal computer 1 are electrically connected, as the driver control built in industrial personal computer 1.
The X-axis of XYZ shaft mechanical mould group 11 includes the first X-axis 26 and the second X-axis 29 being parallel to each other, and Y-axis includes mutually flat
Capable the first Y-axis 27 and the second Y-axis 30, Z axis include the first Z axis 28 and the second Z axis 31 being parallel to each other；First X-axis 26 is horizontal
It is arranged and is connect with pedestal 33, the first Y-axis 27 is vertically arranged and is slidably connected with the first X-axis 26, the first Z axis 28 and the first X-axis
26 are horizontally disposed in 90 ° of angles and are slidably connected with the first Y-axis 27；Second Z axis 31 is slidably connected with the second X-axis 29, the second Y-axis
30 are slidably connected with the second Z axis 31；Second Y-axis 29 is connect with the first Z axis 38 by connecting plate, and setting machinery stirs below connecting plate
Mix device 15；The automatic liquid-feeding arm 21 and automatic liquid absorbing arm 20 being slidably connected are set on the first Z axis 28.
Sample extraction tank includes tank body 5, the first cavity 7 and the second cavity that setting is separated by filtration membrane cartridge 6 in tank body 5
8, upper cover plate 9 is arranged in the top of tank body 5；Filter membrane is set on filtration membrane cartridge 6；Upper cover plate 9 is located at the one of 7 top of the first cavity
Transmission shaft 13 is arranged in side, the agitating screw 23 below transmission shaft 13 in the first cavity 7 of connection insertion, above transmission shaft 13 setting with
The connector 24 of blender connection；Upper cover plate 9 is located at the side setting liquid filling hole 25 of 8 top of the second cavity.
Tank body 5 is made using the plastic material that resistance to organic reagent corrodes；The plastic material of resistance to organic reagent corrosion is poly- for PP
Propylene, PE polyethylene；The volume of tank body 5 is 100cm3；The first cavity 7 of several connections and the second chamber are set on filtration membrane cartridge 6
Filter membrane is arranged on through-hole 10 in the through-hole 10 of body 8；The material selection of filter membrane but it is not limited to nylon, cellulose acetate, polypropylene
Grid, glass fibre, polytetrafluoroethylene (PTFE), nitrocellulose, polyamide.In use, upper cover plate 9 is opened, the sample that needs are extracted
Product are put into the first cavity 7, cover upper cover plate 9, and sample extracting solution is added to the second cavity 8 by liquid filling hole 25 by liquid-adding device
Interior, due to the osmosis of filter membrane, sample extracting solution is penetrated into the first cavity 7, carries out sample incubation；Blender is inserted
Enter in connector 24, blender rotation drives transmission shaft 13 and the agitating screw 23 in the first cavity 7 to rotate；Make the first chamber
Sample in body 7 is sufficiently mixed with sample extracting solution, and the active principle in sample is discharged into sample extracting solution；Dissolved with effective
The sample extracting solution of substance is penetrated into the second cavity 8 by filter membrane, can guarantee to obtain in the second cavity 8 so clear
Sample extracting solution dissolved with active principle, muddy sample mixture stay in the first cavity 7；Liquid absorption device protrudes into liquid filling hole
25 will be handled in the solid phase extraction tube of the sample extracting solution absorption immigration next step dissolved with active principle.
Setting liquid concentration pipe and heating device in liquid concentrator 18, liquid concentration pipe is interior to be arranged hot wind syringe needle 22；Heat
The head bending of wind syringe needle 22, syringe needle hole is opposite with liquid concentration tube wall, so that hot wind on blowing to tube wall, avoids extract liquor
When air-flow caused by liquid splash, sample cross contamination；Heating device and hot wind syringe needle 22 and industrial personal computer 1 are electrically connected, industry control
Machine 1 controls heating device and heats up to liquid concentration pipe, while the hot wind needle blow built in liquid concentration pipe goes out hot wind, to liquid concentration
Pipe carries out interior external heat, and liquid volatilizes in liquid concentration pipe after a period of time, and liquid concentrator 18 cools down, and hot wind syringe needle 22 stops
Blow out hot wind.
12 hole locations are set on metal incubation slot 12, for placing 12 sample extraction tanks；Built in solid phase extraction tube 16
16 quantity of solid phase extraction tube is 12, and liquid concentration pipe quantity is 12, and 22 quantity of hot wind syringe needle is 12, meets 12 samples
While handle.
High-throughput automatic sample pre-treatment instrument also sets up shell 32, and shell 32 is connect with pedestal 1, be arranged on shell 32 into
Wind fan 3 and air ejector fan 2, sample place window 4, and industrial personal computer 1 is arranged on shell 32, air inlet fan 3 and air ejector fan 2 and work
1 point of control machine connection of waking up.
Solid phase extraction tube 16 is formed by the plastic material that resistance to organic reagent corrodes by mold injection, volume 6mL, resistance to have
Plastic material PP polypropylene, PE polyethylene that machine reagent corrodes etc.；16 inner stuffing powder of solid phase extraction tube can be selected and not
It is weak to be limited to HLB hydrophilic-lipophilic balance resin, MCX mixed type cation exchange resin, MAX mixed type anion exchange resin, WCX
Cation exchange resin, WAX weak anion exchange resin, C18 block octadecyl resin, C8 octyl resin, Carb-GCB stone
Inkization carbon black, Flosisil Fo Luoli tripoli, ALA aluminium oxide, ALN aluminium oxide, ALB aluminium oxide, PSA ethylenediamine-N- propyl tree
Rouge is single or is used in mixed way.
Embodiment 1: the automation of flesh of fish Malachite Green is extracted
Window 4 is placed referring to figs. 1 to sample shown in Fig. 9, is opened, the flesh of fish sample for taking the homogeneous of 10g to crush is put into preparatory group
The sample extraction tank installed, sample extraction tank is placed in metal incubation slot 12, is closed sample and is placed window 4, clicking operation work
Control machine 1, selection operation program, system pass through XYZ axis according to the preset project procedure of industrial personal computer 1, by automatic liquid-feeding arm 21
Mechanical mould group 11 is moved to above sample extraction tank, by the liquid filling hole 25 of the liquid feeding head insertion sample extraction tank of automatic liquid-feeding arm 21
In, 50mL sample extracting solution (acetonitrile) is drawn from reagent holding vessel by electromagnetism fluid filling pump 14, is added in liquid filling hole 25.
After the completion of sample extracting solution (acetonitrile) liquid feeding, automatic liquid-feeding arm 21 is removed, and mechanical agitator 15 passes through XYZ shaft mechanical
Mould group 11 is moved to above sample extraction tank, and the agitating screw 23 of mechanical agitator 15 is aligned and is inserted into the company of sample extraction tank
In connector 24,15 motor of mechanical agitator is started turning, and drives the agitating screw 23 of sample extraction tank persistently to stir, sample is mentioned
It takes in tank that liquid and solid are sufficiently stirred, liquid can gradually penetrate the filter membrane 7 of sample extraction tank, flows in the second cavity 8.
According to the preset project procedure of industrial personal computer 1, in whipping process, metal incubation slot 12, which starts to warm up, to be heated to
It 37 DEG C, is stirred for after a certain period of time, the liquid level in the first cavity 7 and the second cavity 8 reaches stable and consistent, the extraction of substance
Reach sufficiently, 15 screw rod of mechanical agitator is removed, metal incubation slot 12 starts to be cooled to room temperature.
According to the preset project procedure of industrial personal computer 1, automatic liquid-feeding arm 21 is moved to solid by XYZ shaft mechanical mould group 11
Above phase extracting tube 16 (neutral alumina SPE column), activating solution (distillation is drawn from reagent holding vessel by electromagnetism fluid filling pump 14
Water) it is added in solid phase extraction tube 16 and is activated, the waste liquid that liquid can be automatically drained out to 16 lower part of solid phase extraction tube returns
It receives in slot 19, after the completion of activation processing, automatic liquid absorbing arm 20 is moved to disposable fluid suction head 17, and disposable fluid suction head 17 is inserted into
In automatic liquid absorbing arm 20, the intracorporal sample extracting solution dissolved with active principle of the second chamber is then drawn, Solid Phase Extraction is added to
In pipe 16, liquid can be automatically drained out into the devil liquor recovery slot 19 of 16 lower part of solid phase extraction tube, and after the completion of activation process, liquid is dense
Contracting device 18 is moved to 16 lower part of solid phase extraction tube, and automatic liquid-feeding arm 21 is moved to 16 top of solid phase extraction tube by electromagnetism fluid filling pump 14
It is added in solid phase extraction tube 16 from absorption extract liquor (ethyl acetate) in reagent holding vessel and carries out extraction processing, extract liquor will be certainly
It is dynamic to flow in liquid concentrator pipe.
In extraction process, liquid in liquid concentrator pipe is concentrated blowout hot wind (temperature control is 60 DEG C), is concentrated
Process will continue into liquid concentrator pipe liquid and volatilize completely, and time control is 10 minutes.
After volatilizing completely, industrial personal computer 1 will issue prompt tone, and work is reminded to be completed, and openable sample placement window 4 will at this time
Liquid concentration pipe and sample extraction tank take out in liquid concentrator 18.
During pre-treatment is all, industrial personal computer 1 can control air inlet fan 3 and air ejector fan 2 is opened, and inside be generated useless
Gas is expelled in external draught cupboard or purification device by air ejector fan 2.Prevent toxic gas or peculiar smell from generating.
Embodiment 2: the automation of Furaxone metabolite is extracted in shrimp
Window 4 is placed referring to figs. 1 to sample shown in Fig. 9, is opened, the shrimp meat sample for taking the homogeneous of 10g to crush originally is put into advance
Assembled sample extraction tank, sample extraction tank is placed in metal incubation slot 12, is closed sample and is placed window 4, clicking operation
Automatic liquid-feeding arm 21 is passed through XYZ according to the preset project procedure of industrial personal computer 1 by industrial personal computer 1, selection operation program, system
Shaft mechanical mould group 11 is moved to above sample extraction tank, by the liquid filling hole of the liquid feeding head insertion sample extraction tank of automatic liquid-feeding arm 21
In 25, pipette samples extracting solution (7ml hydrochloric acid, 0.5mL DMSO is added), addition from reagent holding vessel by electromagnetism fluid filling pump 14
Into liquid filling hole 25.
After the completion of sample extracting solution A (7ml hydrochloric acid, 0.5mL DMSO is added) liquid feeding, automatic liquid-feeding arm 21 is removed, and machinery stirs
It mixes device 15 to be moved to above sample extraction tank by XYZ shaft mechanical mould group 11, the agitating screw 23 of mechanical agitator 15 is aligned
And be inserted into the connector 24 of sample extraction tank, 15 motor of mechanical agitator starts turning, and drives the stirring spiral shell of sample extraction tank
Bar 23 persistently stirs, and liquid in sample extraction tank and solid is sufficiently stirred, liquid can gradually penetrate the mistake of sample extraction tank
Filter membrane 7 flows in the second cavity 8.
According to the preset project procedure of industrial personal computer 1, in whipping process, metal incubation slot 12, which starts to warm up, to be heated to
80 DEG C, after being stirred for certain time (10 minutes), the liquid level in the first cavity 7 and the second cavity 8 reaches stable and consistent, object
The extraction of matter reaches sufficiently, and 15 screw rod of mechanical agitator is removed, and metal incubation slot 12 starts to be cooled to room temperature.
It is added after the completion of sample extracting solution B (1ml sodium hydroxide, 10mL PBS buffer solution is added) liquid feeding, automatic liquid-feeding arm
21 remove, and mechanical agitator 15 is moved to above sample extraction tank by XYZ shaft mechanical mould group 11, by stirring for mechanical agitator 15
It mixes screw rod 23 to be aligned and be inserted into the connector 24 of sample extraction tank, 15 motor of mechanical agitator starts turning, and sample is driven to mention
The agitating screw 23 of tank is taken persistently to stir.
According to the preset project procedure of industrial personal computer 1, automatic liquid-feeding arm 21 is moved to solid by XYZ shaft mechanical mould group 11
16 top of phase extracting tube 16 (HLB SPE column), activating solution (methanol) addition is drawn by electromagnetism fluid filling pump 14 from reagent holding vessel
It is activated into solid phase extraction tube 16, liquid can be automatically drained out to the devil liquor recovery slot 19 of 16 lower part of solid phase extraction tube
In, after the completion of activation processing, automatic liquid absorbing arm 20 is moved to disposable fluid suction head 17, and disposable fluid suction head 17 is inserted into automatic suction
In liquid arm 20, the intracorporal liquid of the second chamber is then drawn, is added in solid phase extraction tube 16, liquid, which can be automatically drained out to solid phase, to be extracted
In the devil liquor recovery slot 19 for taking 16 lower part of pipe, after the completion of activation process, liquid concentrator 18 is moved to 16 lower part of solid phase extraction tube,
Automatic liquid-feeding arm 21 is moved to 16 top of solid phase extraction tube and draws extract liquor (ammonification from reagent holding vessel by electromagnetism fluid filling pump 14
Methanol) it is added in solid phase extraction tube 16 and carries out extraction processing, extract liquor will be flow in liquid concentrator pipe automatically.
In extraction process, liquid in liquid concentrator pipe is concentrated blowout hot wind (temperature control is 60 DEG C), is concentrated
Process will continue into liquid concentrator pipe liquid and volatilize completely, and time control is 10 minutes.
After volatilizing completely, industrial personal computer 1 will issue prompt tone, and work is reminded to be completed, and openable sample placement window 4 will at this time
Liquid concentration pipe and sample extraction tank take out in liquid concentrator 18.
During pre-treatment is all, industrial personal computer 1 can control air inlet fan 3 and air ejector fan 2 is opened, and inside be generated useless
Gas is expelled in external draught cupboard or purification device by air ejector fan 2.Prevent toxic gas or peculiar smell from generating.
Embodiment 3: the automation of clenobuterol hydrochloride is extracted in pork
Window 4 is placed referring to figs. 1 to sample shown in Fig. 9, is opened, the pork sample for taking the homogeneous of 5g to crush is put into pre-assembly
Good sample extraction tank, sample extraction tank is placed in metal incubation slot 12, is closed sample and is placed window 4, clicking operation industry control
Machine 1, selection operation program, system pass through XYZ axis machine according to the preset project procedure of industrial personal computer 1, by automatic liquid-feeding arm 21
Tool mould group 11 is moved to above sample extraction tank, by the liquid filling hole 25 of the liquid feeding head insertion sample extraction tank of automatic liquid-feeding arm 21
In, 50mL sample extracting solution (phosphate buffer) is drawn from reagent holding vessel by electromagnetism fluid filling pump 14, is added to liquid filling hole
In 25.
After the completion of sample extracting solution (phosphate buffer) liquid feeding, automatic liquid-feeding arm 21 is removed, and mechanical agitator 15 passes through
XYZ shaft mechanical mould group 11 is moved to above sample extraction tank, and the agitating screw 23 of mechanical agitator 15 is aligned and is inserted into sample
In the connector 24 of extractor, 15 motor of mechanical agitator is started turning, and the agitating screw 23 of sample extraction tank is driven persistently to stir
It mixes, liquid in sample extraction tank and solid is sufficiently stirred, liquid can gradually penetrate the filter membrane 7 of sample extraction tank, flowed to
In second cavity 8.
According to the preset project procedure of industrial personal computer 1, in whipping process, metal incubation slot 12, which starts to warm up, to be heated to
100 DEG C, after being stirred for certain time (10 minutes), the liquid level in the first cavity 7 and the second cavity 8 reaches stable and consistent,
The extraction of substance reaches sufficiently, and 15 screw rod of mechanical agitator is removed, and metal incubation slot 12 starts to be cooled to room temperature.
According to the preset project procedure of industrial personal computer 1, automatic liquid-feeding arm 21 is moved to solid by XYZ shaft mechanical mould group 11
16 top of phase extracting tube 16 (PSA column), is drawn activating solution (2% acetic acid) from reagent holding vessel by electromagnetism fluid filling pump 14 and is added to
It being activated in solid phase extraction tube 16, liquid can be automatically drained out into the devil liquor recovery slot 19 of 16 lower part of solid phase extraction tube,
After the completion of activation processing, automatic liquid absorbing arm 20 is moved to disposable fluid suction head 17, and disposable fluid suction head 17 is inserted into automatic imbibition
In arm 20, the liquid in the second cavity 8 is then drawn, is added in solid phase extraction tube 16, liquid, which can be automatically drained out to solid phase, to be extracted
In the devil liquor recovery slot 19 for taking 16 lower part of pipe, after the completion of activation process, liquid concentrator 18 is moved to 16 lower part of solid phase extraction tube,
Automatic liquid-feeding arm 21 is moved to 16 top of solid phase extraction tube and draws extract liquor (50% from reagent holding vessel by electromagnetism fluid filling pump 14
Ethyl alcohol) it is added in solid phase extraction tube 16 and carries out extraction processing, extract liquor will be flow in liquid concentration pipe automatically.
Liquid can be directly used for detecting in liquid concentrator pipe, does not need progress nitrogen and blows step.
Embodiment 4: the automation of aflatoxin B1 is extracted in corn flour
Window 4 is placed referring to figs. 1 to sample shown in Fig. 9, is opened, the corn flour sample for taking the homogeneous of 5g to crush is put into preparatory group
The sample extraction tank installed, sample extraction tank is placed in metal incubation slot 12, is closed sample and is placed window 4, clicking operation work
Control machine 1, selection operation program, system pass through XYZ axis according to the preset project procedure of industrial personal computer 1, by automatic liquid-feeding arm 21
Mechanical mould group 11 is moved to above sample extraction tank, by the liquid filling hole 25 of the liquid feeding head insertion sample extraction tank of automatic liquid-feeding arm 21
In, it is drawn 50mL sample extracting solution (80% ethyl alcohol), is added in liquid filling hole 25 from reagent holding vessel by electromagnetism fluid filling pump 14.
After the completion of sample extracting solution (80% ethyl alcohol) liquid feeding, automatic liquid-feeding arm 21 is removed, and mechanical agitator 15 passes through XYZ axis
Mechanical mould group 11 is moved to above sample extraction tank, and the agitating screw 23 of mechanical agitator 15 is aligned and is inserted into sample extraction tank
Connector 24 in, 15 motor of mechanical agitator starts turning, drive sample extraction tank agitating screw 23 persistently stir, by sample
Liquid and solid are sufficiently stirred in product extractor, liquid can gradually penetrate the filter membrane 7 of sample extraction tank, flow to the second cavity
In 8.
According to the preset project procedure of industrial personal computer 1, in whipping process, metal incubation slot 12 does not need to heat, temperature
Spending constant is room temperature, and after being stirred for certain time (10 minutes), the liquid level of the first cavity 7 and the second cavity 8 reaches stable
Unanimously, the extraction of substance reaches sufficiently, and 15 screw rod of mechanical agitator is removed.
According to the preset project procedure of industrial personal computer 1, automatic liquid-feeding arm 21 is moved to solid by XYZ shaft mechanical mould group 11
16 top of phase extracting tube 16 (aflatoxin B1 immunoabsorbent column), activation is drawn by electromagnetism fluid filling pump 14 from reagent holding vessel
Liquid (phosphate buffer), which is added in solid phase extraction tube 16, to be activated, and liquid can be automatically drained out to solid phase extraction tube 16
In the devil liquor recovery slot 19 of lower part, after the completion of activation processing, automatic liquid absorbing arm 20 is moved to disposable fluid suction head 17, will be disposable
Fluid suction head 17 is inserted into automatic liquid absorbing arm 20, is then drawn the liquid in the second cavity 8, is added in solid phase extraction tube 16, liquid
Cognition is automatically drained out into the devil liquor recovery slot 19 of 16 lower part of solid phase extraction tube, and after the completion of activation process, liquid concentrator 18 is moved
It moves to 16 lower part of solid phase extraction tube, automatic liquid-feeding arm 21 is moved to 16 top of solid phase extraction tube and is stored up by electromagnetism fluid filling pump 14 from reagent
It deposits and draws extract liquor (methanol) in tank and be added in solid phase extraction tube 16 and carry out extraction processing, it is dense that extract liquor will flow to liquid automatically
In contracting device pipe.
In extraction process, liquid in liquid concentrator pipe is concentrated blowout hot wind (temperature control is 60 DEG C), is concentrated
Process will continue into liquid concentrator pipe liquid and volatilize completely, and time control is 10 minutes.
After volatilizing completely, industrial personal computer 1 will issue prompt tone, and work is reminded to be completed, and openable sample placement window 4 will at this time
Liquid concentration pipe and sample extraction tank take out in liquid concentrator 18.
During pre-treatment is all, industrial personal computer 1 can control air inlet fan 3 and air ejector fan 2 is opened, and inside be generated useless
Gas is expelled in external draught cupboard or purification device by air ejector fan 2.Prevent toxic gas or peculiar smell from generating.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
There is also other identical elements in process, method, article or equipment including the element.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (9)
1. a kind of high throughput automatic sample pre-treatment instrument, it is characterised in that: be incubated for including pedestal, XYZ shaft mechanical mould group, metal
Slot, sample extraction tank, Solid Phase Extraction pipe holder, imbibition head bracket, liquid concentrator, devil liquor recovery slot, electromagnetism fluid filling pump and work
Control machine；XYZ shaft mechanical mould group is set on the pedestal, and the XYZ shaft mechanical mould group includes X-axis, Y-axis and Z axis, the X-axis water
Flat to be arranged and connect with pedestal, Y-axis is vertically arranged and is slidably connected with X-axis, and the Z axis and X-axis are horizontally disposed simultaneously in 90 ° of angles
It is slidably connected with Y-axis；Mechanical agitator, automatic liquid absorbing arm and automatic liquid-feeding arm are set on the Z axis；The bottom of the X-direction
Devil liquor recovery slot is set on seat, metal incubation slot is set above the devil liquor recovery slot, the sample extraction tank is incubated set on metal
It educates in slot；The Solid Phase Extraction pipe holder is set side by side with metal incubation slot, and solid phase extraction tube is arranged in Solid Phase Extraction pipe holder；
The imbibition head bracket is set to metal incubation slot side, the disposable fluid suction head of setting in imbibition head bracket；The liquid concentration
Device is movably arranged between Solid Phase Extraction pipe holder and devil liquor recovery slot by track；The electromagnetism fluid filling pump is set on the base simultaneously
It is connect with automatic liquid-feeding arm；The XYZ shaft mechanical mould group, mechanical agitator, electromagnetism fluid filling pump automatic liquid-feeding arm, automatic liquid absorbing arm
It is electrically connected with industrial personal computer.
2. a kind of high-throughput automatic sample pre-treatment instrument according to claim 1, it is characterised in that: further, institute
The X-axis for stating XYZ shaft mechanical mould group includes the first X-axis and the second X-axis being parallel to each other, Y-axis include the first Y-axis being parallel to each other and
Second Y-axis, Z axis include the first Z axis and the second Z axis being parallel to each other；First X-axis is horizontally disposed and connect with pedestal, the
One Y-axis is vertically arranged and is slidably connected with the first X-axis, and first Z axis and the first X-axis are horizontally disposed in 90 ° of angles and with the
One Y-axis is slidably connected；Second Z axis is slidably connected with the second X-axis, and second Y-axis is slidably connected with the second Z axis；It is described
Second Y-axis is connect with the first Z axis by connecting plate, and mechanical agitator is arranged below the connecting plate；It is arranged on first Z axis
The automatic liquid-feeding arm and automatic liquid absorbing arm being slidably connected.
3. a kind of high-throughput automatic sample pre-treatment instrument according to claim 1, it is characterised in that: the sample extraction tank
Including tank body, the first cavity and the second cavity that setting is separated by filtration membrane cartridge in the tank body, the top of the tank body is set
Set upper cover plate；Filter membrane is set on the filtration membrane cartridge；The upper cover plate is located at the setting transmission of the side above the first cavity
Axis, the transmission shaft lower section connection insertion intracorporal agitating screw of the first chamber, the company that the setting of transmission shaft top is connect with blender
Connector；The upper cover plate is located at the setting of the side above the second cavity liquid filling hole；The tank body is corroded using resistance to organic reagent
Plastic material production；The plastic material of the resistance to organic reagent corrosion is PP polypropylene, PE polyethylene；The volume of tank body is
100cm3；The through-hole of several connection the first cavitys and the second cavity is set on filtration membrane cartridge, filter membrane is set on the through-hole；
The material selection of filter membrane but to be not limited to nylon, cellulose acetate, polypropylene lattice, glass fibre, polytetrafluoroethylene (PTFE), nitric acid fine
Dimension element, polyamide.
4. a kind of high-throughput automatic sample pre-treatment instrument according to claim 1, it is characterised in that: the liquid concentrator
Interior setting liquid concentration pipe and heating device, the liquid concentration pipe is interior to be arranged hot wind syringe needle；It rolls on the head of the hot wind syringe needle
Curved, syringe needle hole is opposite with liquid concentration tube wall；The heating device and hot wind syringe needle and industrial personal computer are electrically connected.
5. a kind of high-throughput automatic sample pre-treatment instrument according to claim 1, it is characterised in that: the metal incubation slot
12 hole locations of upper setting, for placing 12 sample extraction tanks；Solid phase extraction tube quantity built in the solid phase extraction tube is 12
A, liquid concentration pipe quantity is 12, and the hot wind syringe needle quantity is 12.
6. according to claim 1 a kind of high-throughput automatic sample pre-treatment with, it is characterised in that: it is described it is high-throughput entirely from
Dynamic sample pre-treatments instrument also sets up shell, and the shell is connect with pedestal, setting air inlet fan and air ejector fan on the shell,
Sample places window, and the industrial personal computer setting is on the shell.
7. a kind of high-throughput automatic sample pre-treatment instrument according to claim 1, it is characterised in that: the solid phase extraction tube
It is formed by the plastic material that resistance to organic reagent corrodes by mold injection, volume 6mL；The solid phase extraction tube inner stuffing powder
HLB hydrophilic-lipophilic balance resin, MCX mixed type cation exchange resin, MAX mixed type anion can be selected and be not limited in end
Exchanger resin, WCX weak cation exchange resin, WAX weak anion exchange resin, C18 block octadecyl resin, C8 octyl tree
Rouge, Carb-GCB Graphon, Flosisil Fo Luoli tripoli, ALA aluminium oxide, ALN aluminium oxide, ALB aluminium oxide, PSA second
Two amine-n-propyl resins are single or are used in mixed way.
8. a kind of any one of the claim 1-7 high-throughput automatic sample pre-treatment instrument extract food or raw-food material,
Or pesticide in Chinese medicine, additive, illegal additive, coloring agent, weighting agent, clenbuterol hydrochloride, drug, the application in bacterium.
9. a kind of application method of high throughput automatic sample pre-treatment instrument, it is characterised in that the following steps are included:
S1, sample liquid feeding, are controlled by industrial personal computer, are driven by electromagnetism fluid filling pump, are added sample extracting solution by automatic liquid-feeding arm
Enter into sample extraction tank；
S2, sample incubation, are controlled by industrial personal computer, the heating of metal incubation slot, the solidliquid mixture heating one in sample extraction tank
After fixing time, active principle in sample is sufficiently discharged into extracting solution；
S3, sample stirring, are controlled by industrial personal computer, drive XYZ shaft mechanical mould group, the stirring-head of mechanical agitator is inserted into sample
In product extractor connecting shaft, agitating screw starts high-speed rotation, and solid and liquid in sample extraction tank are adequately mixed,
Active principle in sample is sufficiently discharged into sample extracting solution；
S4, sample filtering are completed in sample extraction tank, and clear sample extracting solution can be by the sample installed in sample extraction tank
Product extractor filter membrane, the interior solidliquid mixture for muddiness of the first cavity, interior the second cavity is the sample dissolved with active principle
Extracting solution；
S5, solid-phase extraction column activation, are controlled by industrial personal computer, drive XYZ shaft mechanical mould group, driven, passed through by electromagnetism fluid filling pump
Activating solution is added in solid phase extraction tube by automatic liquid-feeding arm, and the liquid of outflow is directly entered devil liquor recovery slot；
S6, sample liquid loading, are controlled by industrial personal computer, drive XYZ shaft mechanical mould group, and automatic liquid absorbing arm will dissolve in the second cavity
There is the sample extracting solution absorption of active principle to be added in solid phase extraction tube, the liquid of outflow is directly entered devil liquor recovery slot；
S7, Solid Phase Extraction column purification, are controlled by industrial personal computer, are driven XYZ shaft mechanical mould group, are driven, passed through by electromagnetism fluid filling pump
Scavenging solution is added in solid phase extraction tube by automatic liquid-feeding arm, and the liquid of outflow is directly entered devil liquor recovery slot；
S8, sample liquid recycling, are controlled by industrial personal computer, drive XYZ shaft mechanical mould group, driven by electromagnetism fluid filling pump, by automatic
Scavenging solution is added in solid phase extraction tube by liquid adding arm, and the liquid of outflow enters in liquid concentrator pipe；
S9, sample liquid concentration, are controlled by industrial personal computer, liquid concentrator heating, while the hot wind syringe needle built in liquid concentrator
Hot wind is blown out, interior external heat is carried out to the concentration tube in liquid concentrator, liquid volatilizes in concentration tube after a period of time, and liquid is dense
The cooling of contracting device, hot wind syringe needle stop blowout hot wind.
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| CN201910903132.8A CN110514500A (en) | 2019-09-24 | 2019-09-24 | A kind of high throughput automatic sample pre-treatment instrument, application and application method |
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| CN113063648A (en) * | 2021-04-21 | 2021-07-02 | 北京莱伯泰科仪器股份有限公司 | Ice bath sample holder device for homogenization and homogenization platform thereof |
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| CN114152496A (en) * | 2021-12-31 | 2022-03-08 | 河南赛诺特生物技术有限公司 | Automatic immunohistochemical dyeing machine |
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