CN101923096A - Automatic westem blot analyzer - Google Patents
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- CN101923096A CN101923096A CN200910052971XA CN200910052971A CN101923096A CN 101923096 A CN101923096 A CN 101923096A CN 200910052971X A CN200910052971X A CN 200910052971XA CN 200910052971 A CN200910052971 A CN 200910052971A CN 101923096 A CN101923096 A CN 101923096A
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Abstract
The invention discloses an automatic westem blot analyzer, comprising an incubating swing bed, a liquid adding arm component, a displacement motor, a liquid sucking arm component, a liquid sucking motor, an alarm-carrying negative pressure liquid sucking pump and a plurality of liquid adding digital pumps, wherein, the incubating swing bed is provided with a plurality of incubating grooves; the liquid adding arm component and the liquid sucking arm component are arranged on a gliding mechanical device and respectively add test solution and suck waste liquid to the incubating grooves in the incubating swing bed; the liquid adding arm component is connected with the displacement motor and the liquid adding digital pump; the liquid sucking arm component is connected with the liquid sucking motor and the alarm-carrying negative pressure liquid sucking pump. The analyzer of the invention can automatically complete a series of operations, such as preparing liquid, sucking liquid, incubating, washing and adding substrate liquid and the like, processes each westem blot detection and analysis in batch, and can reduce the working intensity of operators and save the time.
Description
Technical field
The present invention relates to a kind of protein blot experiment device, particularly relate to a kind of automatic westem blot (Western Blot) analyser.
Background technology
Detected by Western blot is a kind of effective ways by specific antibody evaluation antigen, and this method is a kind of new immune biochemical technology that grows up on gel electrophoresis and solid-phase immunoassay technical foundation.Detected by Western blot is that the protein component of electrophoretic separation is transferred to a kind of solid support from gel, detect by antibody and the epitope generation specific immune response that target protein presented that is attached to solid support, whole process comprises: protein electrophorese separates, electrotransfer, immunology detection and input four parts.At present, immune detection partly comprises following process in the existing protein blot experiment: sealing, and the first antibody combination is cleaned, and the second antibody combination is cleaned.The reagent that needs to handle in this part experiment flow is confining liquid, first antibody dilution, cleaning buffer solution, second antibody dilution, cleaning buffer solution.What adopt at present is that manual method is changed different reagent, frequent change the liquid operation and has increased experimenter's working strength and lose time.
Simultaneously, it is to adopt by hand each operation steps is become complete detection continuously that Western blot detects classic method, and shortcoming is loaded down with trivial details and complicated, and the people is many with contacting of tool latent infection blood article, manual operation difference and environmental change and the test findings difference that causes, the working strength height.
Summary of the invention
The technical problem to be solved in the present invention is in order to overcome the defective of prior art, a kind of automatic westem blot analyzer is provided, this analyser can automatically finish dosing, imbibition, hatch, wash and add sequence of operations such as substrate solution, each Western blot check and analysis of batch processing can reduce operator's working strength and save time.
The present invention solves above-mentioned technical matters by following technical proposals: a kind of automatic westem blot analyzer, it is characterized in that, it comprises hatches shaking table, the liquid adding arm parts, the displacement motor, the liquid absorbing arm parts, the imbibition motor, band warning negative pressure suction pumps and a plurality of liquid feeding digital pump, hatch shaking table and have a plurality of incubation slots, liquid adding arm parts and liquid absorbing arm parts are installed on the sliding mechanical device and respectively the incubation slot of hatching in the shaking table are added test solution and inhales waste liquid, liquid adding arm parts and displacement motor, the liquid feeding digital pump connects, liquid absorbing arm parts and imbibition motor, band warning negative pressure suction pumps connects.
Preferably, described automatic westem blot analyzer also comprises a cage, and this cage covers shaking table parts, liquid adding arm parts, liquid absorbing arm parts and sliding mechanical device fully.
Preferably, described liquid feeding digital pump is connected with a bottle for handling liquid toilet or cosmetic substance or a reagent bottle.
Preferably, described liquid adding arm parts comprise that liquid feeding chews and the liquid feeding sebific duct, and liquid feeding is chewed an end that connects the liquid feeding sebific duct, and the other end of liquid feeding sebific duct is connected with digital fluid filling pump.
Positive progressive effect of the present invention is: automatic westem blot analyzer of the present invention is automatically each operation steps to be become complete check and analysis continuously under computer control, simple to operate, report to the police the automaticity height automatically, but unmanned, the western blot analysis standardization.And, the present invention is reduced to a minimum the people with contacting of tool latent infection blood article, make the immunoblotting assay standardization, reduced the analysis result difference that causes because of the laboratory environment variation effectively, its accurate dosing and imbibition system guarantee the consistance of analysis result, and prevent the cross pollution between sample cell.
Description of drawings
Fig. 1 is the module frame chart of automatic westem blot analyzer of the present invention.
Fig. 2 is the structural representation of automatic westem blot analyzer of the present invention.
Fig. 3 is the structural representation of hatching shaking table among the present invention.
Fig. 4 is the liquid adding arm parts among the present invention and the structural representation of liquid absorbing arm parts.
Fig. 5 is the structural representation of the sliding mechanical device among the present invention.
Fig. 6 is the structural representation of the liquid feeding digital pump among the present invention.
Embodiment
Provide preferred embodiment of the present invention below in conjunction with accompanying drawing, to describe technical scheme of the present invention in detail.
As shown in Figure 1, automatic westem blot analyzer of the present invention mainly comprises hatches shaking table 1, liquid adding arm parts 4, displacement motor 5, liquid absorbing arm parts 6, imbibition motor 7, band warning negative pressure suction pumps 8 and a plurality of liquid feeding digital pump 9 (adopting six among the present invention), hatch shaking table 1 for having the shaking table of 36 incubation slots, it comprises interconnective shaking table motor 2 and shaking table parts 3, the function of hatching shaking table 1 is under software control, evenly wave applying the examination film bar that in the disposable incubation slot in shaking table surface, adds test solution, so that test solution evenly covers examination film bar in the groove, the shaking table plane has the temperature function of adding.Liquid adding arm parts 4 are six passage liquid adding arm parts, it has, and 36 incubation slots (disposable) divide groove to add the function of test solution in the shaking table 1 to hatching, liquid adding arm parts 4 are connected with six liquid feeding digital pump 9 and make up, under software control, have at most to reach digital quantitative liquid feeding selection within six kinds of different test solutions selections and the 50-3000uL.Liquid adding arm parts 4 comprise that liquid feeding support arm, liquid feeding chew and the liquid feeding sebific duct, and the liquid feeding sebific duct can be connected with liquid feeding digital pump 9, and liquid adding arm parts 4 are connected with displacement motor 5, and provide power by displacement motor 5 to liquid adding arm parts 4.The function of liquid absorbing arm parts 6 is to after the test solution reaction that examination film bar adds in 36 incubation slots in the shaking table, under software control, the waste liquid of the negative pressure liquid sucking device that liquid absorbing arm parts 6 and band warning negative pressure suction pumps 8 are formed after to the groove internal reaction inhaled rapidly and gone, and imbibition motor 7 is connected with liquid absorbing arm parts 6 and power is provided.Liquid adding arm parts 4 and liquid absorbing arm parts 6 are mounted on the sliding mechanical device, under software control, can accurately aim at and hatch dish 36 grooves, so that add test solution and inhale waste liquid.
As shown in Figure 2, the present invention also comprises liquid feeding digital pump motor 10, bottle for handling liquid toilet or cosmetic substance 11, reagent bottle 12, waste liquid bottle 13 and null pick-up 14, each liquid feeding digital pump motor 10 corresponding liquid feeding digital pump 9 also provides power for liquid feeding digital pump 9, a liquid feeding digital pump 9 is connected with bottle for handling liquid toilet or cosmetic substance 11, all the other five liquid feeding digital pump 9 are connected with reagent bottle 12 respectively and extract reagent, waste liquid is sucked in the waste liquid bottle 13 by band warning negative pressure suction pumps 8 by liquid absorbing arm parts 6, and null pick-up 14 is used to detect the rotational angle of imbibition motor 7.Certainly, the present invention can also comprise a cage, and this cage can cover shaking table parts, liquid adding arm parts, liquid absorbing arm parts and sliding mechanical device fully, play workplace and external security and isolate, but the cage folding.
As shown in Figure 3, shaking table parts 3 comprise shaker platform 31, heating module 32, sensor 33, eccentric stiffener 34, platform bracket and eccentric stent constitute, shaker platform 31 is fixed on the platform bracket, heating module 32 is clamped in the centre of shaker platform 31 and platform bracket, heating module 32 can be shaker platform 31 three kinds of thermostat temperatures is provided, platform bracket two ends are installed on respectively on two shaker stents, shaker stent is fixed on the large platform, shaker platform 31 can swing along shaker stent, eccentric stent is fixed in platform bracket bottom one end, eccentric stent lower end Y mouth inserts the eccentric stiffener 34 at the large platform back side, eccentric stiffener 34 provides kinetic energy by shaking table motor 2, shaking table motor 2 rotates and drives eccentric stiffener 34 rotations, eccentric stiffener 34 rotates and drives the bent axle motion up and down of eccentric stent Y mouth do, shaker platform 31 is swung, realize the shaking table function.1 covers disposable 36 grooves (moulding system) of hatching on shaker platform 3, all Western blots detections, liquid feeding, hatch, combination, application of sample, cleaning etc. all carry out in groove, can carry out 36 sample detection simultaneously.
As shown in Figure 4 and Figure 5, liquid adding arm parts 4 and liquid absorbing arm parts 6 are installed on the sliding mechanical device, the sliding mechanical device comprise slip major axis 81, long axis frame, mobile motor 82, synchronizing wheel, movable support bracket parts, synchronously with 83, mobile motor 82 sides are positioned at first sensor 84 and are positioned at second sensor 85 of liquid absorbing arm parts 6 sides.Has axle sleeve in the movable support bracket parts, axle sleeve is in the slip major axis, the movable support bracket parts can slide in the slip major axis, liquid adding arm parts 4 and liquid absorbing arm parts 6 are to be fixed on the movable support bracket parts, long axis frame is supported on major axis on the table top, be inserted in mobile motor 82 shafts at major axis two ends synchronizing wheel, load onto and be with 83 synchronously, be with 83 to fix with the movable support bracket parts synchronously on one side, when control mobile motor 82 rotates, promptly drive synchronizing wheel and be with 83 to move synchronously, the carriage member straight line is moved around, just liquid adding arm parts 4 and liquid absorbing arm parts 6 move around, thereby realize dividing the groove positioning function to hatching dish one to 36 groove liquid feeding and imbibition, first sensor 84 and second sensor, 85 feedback positioning states.Liquid adding arm parts 4 comprise that the liquid feeding that liquid feeding support arm 41, liquid feeding sebific duct and six being aligneds are arranged chews 42, liquid adding arm parts 4 are fixed on the movable support bracket parts of sliding mechanical device, liquid feeding is chewed 42 top can connect liquid feeding sebific duct one end, the liquid feeding sebific duct other end is connected with digital fluid filling pump 9, chew 42 by digital fluid filling pump 9 to liquid feeding the setting test solution is provided, it is liquid outlet that liquid feeding is chewed 42 bottom, and six liquid feedings are chewed 42 and formed six passages and add the test solution passage.Liquid absorbing arm parts 6 comprise that the imbibition support arm 61 that cooperatively interacts and chute support arm 62 and imbibition chew and the imbibition sebific duct, the axis sliding sleeve of imbibition support arm 61 is in the slip major axis 81 of sliding mechanical device, can go up lower edge axle core and move, as stretching into incubation slot imbibition job requirement.An end that is fixed in imbibition support arm 61 is chewed in imbibition, and top is chewed in imbibition can connect imbibition sebific duct one end, and the imbibition sebific duct other end communicates with the negative pressure waste liquid bottle.The chute support arm 62 of liquid absorbing arm parts is fixed on the shaft of imbibition motor 7 by central shaft hole, imbibition motor 7 turns an angle, driving chute support arm 62 rotates, make at chute support arm 62 upper hopper chutes, driving the slip major axis 81 of imbibition support arm 61 axis with matching along sliding sleeve in the sliding mechanical device moves up and down, control imbibition motor 7 rotational angles, so just control the movement locus of liquid absorbing arm parts 6.Null pick-up 14 is fixed in chute support arm 62 1 ends, is used to detect the rotational angle of imbibition motor 7.
As shown in Figure 6, liquid feeding digital pump 9 comprises pump impeller, pressure arm 91, elasticity button 92, pump line 93, feed tube 94, drain pipe, installing plate and pump motor, pump impeller is inserted in the pump motor shaft, pump motor is fixed on the installing plate, and pressure arm 91 1 ends are connected in the installing plate bottom righthand side with connecting shaft, and pressure arm 91 can be rotated along connecting shaft, at the installing plate left end, with elasticity button 92 1 ends in addition part be fixed on the installing plate, elasticity button 92 other ends can about move along the upper end so that the opening of pressure arm 91 1 ends incision location.When pump line 93 is put on the pump impeller, with locking in the pressure arm 91 1 end openings incision elasticity button 92, pump line 93 is crushed on pump impeller under pressure arm 91 effects, pump line 93 right-hand members put feed tube 94 1 ends, feed tube 94 other ends stretch into reagent bottle 12, pump line 93 left ends put drain pipe one end, the other end is chewed with the liquid feeding of liquid adding arm and is connected, when the control pump motor rotates, drive the extruding of pump impeller to pump line 93, producing negative pressure, suct test solution from reagent bottle 12 by pipeline, from fluid B mouth the liquid feeding that test solution is transported to liquid adding arm is chewed, pump motor is the digitizing stepper motor, and the control pump motor rotates, make the liquid outlet quantity of pump, by software control, the liquid feeding digital pump can be set arbitrarily from 1-3000uL in theory, and its degree of accuracy can be demarcated respectively by every pump.The liquid feeding digital pump not only can up time be changeed, as operate as normal, but also inverse time commentaries on classics, as test solution in the reclaim line.
The present invention has automatic liquid matching, imbibition, hatch, flushing, add substrate solution, add bond and automatic cleaning function, when the present invention carries out the detection of immune protein trace detection (Western Blotting) method, the present invention can be to the dosing of Western blot detection, imbibition, hatch, flushing, add substrate solution, add bond, functions such as cycle index and automatic cleaning, by configuring application software of the present invention, by the indicate parameter of operator according to various Western blots (Western Blotting) kit, import in the configuring application software of the present invention, work out various test events, various test events can standing storage, also can revise.Test event comprises 20 independent processes at most, can be to incubation time in each independent process, additional examination liquid measure, inhale not imbibition, work step warning and cycle index and the step title is set.The present invention is furnished with the liquid adding arm parts, by programming Control, according to Western blot (Western Blotting) testing requirements, in same test, can add six kinds of different test solutions at most separately respectively.After each Western blot examination film bar and the reaction of certain test solution, its useless test solution need be collected in spy of the present invention joins in the waste liquid device, and the present invention is furnished with special imbibition and waste collection device.Operating platform of the present invention is shaking table parts, and the shaking table parts have three kinds and wave rate selection, by software control of the present invention.The present invention is covered on shaking table and is moulded disposable 36 incubation slots of system, and test solution and ProBloot membrane bar all add or put into incubation slot hatches and react, and the shaking table parts have three kinds of differences and add temperature function.Liquid adding arm parts of the present invention and liquid absorbing arm parts are installed on the same sliding mechanical device, displacement motor on the control sliding mechanical device, just can make liquid adding arm and liquid absorbing arm aim at the disposable any groove position of hatching in dish one to 36 groove of shaking table surface coverage, and the installation site only differs from a groove position before and after liquid adding arm and the liquid absorbing arm, liquid absorbing arm is preceding, liquid adding arm after, a groove position after aligning is chewed in the suction of liquid absorbing arm, then the liquid feeding of liquid adding arm is chewed and is aimed at previous groove position, when test solution was discarded in P1 reaction back in one groove position, suction back is chewed in the liquid absorbing arm suction, liquid adding arm was set the P2 test solution to previous groove position adding program simultaneously.In sum, the present invention is made of shaking table parts, liquid adding arm parts, imbibition and waste collection part device, the mechanical part device of displacement and electronic software system etc.Liquid adding arm parts and liquid absorbing arm parts are installed on the sliding mechanical device, only need the Digital Control stepper motor, just can accurately control liquid adding arm assembly and liquid absorbing arm assembly location, suit individually, select arbitrarily in batches, carry out simultaneously before and after imbibition and the liquid feeding, shorten operating process.The liquid adding arm parts are made up of six independent liquid feeding digital pump and liquid adding arm and sebific duct, and the liquid feeding digital pump is the Electromechanical Design parts, extract digitizing, easy cleaning.Imbibition and waste collection are under full closeding state, and be pollution-free.
After the present invention enters the washing lotion step, displacement motor on the software control sliding mechanical, making it liquid adding arm on the sliding mechanical moves and aims on the shaking table on the 36 incubation slot setting slots, one of them sets the rotation of liquid feeding digital pump software control, and the liquid feeding digital pump quantitatively pumps the setting test solution in the P1 reagent bottle of setting, be transported on the liquid adding arm by connecting line and set liquid feeding in six passages and chew, add on the shaking table and set in the incubation slot, add test solution, the liquid feeding digital pump stops operating.Software continues control sliding mechanical superior displacement motor, make it liquid adding arm on the sliding mechanical and move to and hatch on next groove position of dish, software is controlled the liquid feeding digital pump again and is rotated, and the liquid feeding digital pump is in the P1 reagent bottle of setting, quantitatively pump the setting test solution, add shaking table and hatch in next groove of dish.So add test solution, till setting the N groove in addition.If the work step setting need be in the discharge opeing of step end of run in this step, then shaking table wave hatch after, displacement motor on the software control sliding mechanical, making it liquid absorbing arm on the sliding mechanical moves and aims on the shaking table in the 36 incubation slot setting slots, start band warning negative pressure suction pumps simultaneously, make to be attached thereto and set up negative pressure in the waste liquid bottle, make on the liquid absorbing arm to inhale and chew mouth and be in negative pressure state, waste liquid after the reaction in the incubation slot is sucked in the waste liquid bottle.Characteristics of the present invention are in the imbibition process, carry out the liquid feeding work step of next step simultaneously, this is because liquid absorbing arm and liquid adding arm are installed on the same axle sliding mechanical, and only differs from a groove position, so after liquid absorbing arm was inhaled and removed this step waste liquid, liquid adding arm was carried out next step liquid feeding work step simultaneously following closely.These two work steps carry out simultaneously, and this step cycle number of times can be set.
After the present invention entered the sample step, the software control buzzing was reported to the police, and cage on the shaking table is opened in prompting " adding sample ", added blood sample.By " YES " key, prompting " closing cage ", again by " YES " key, shaking table drives hatches dish, waving under the speed of setting, enters incubation time, and the shaking table plane is by the software control temperature.When incubation time finishes, displacement motor on the software control sliding mechanical, 6 make it liquid absorbing arm on the sliding mechanical moves and aims on the shaking table in the 36 incubation slot setting slots, start negative pressure pump simultaneously, make to be attached thereto and set up negative pressure in the waste liquid bottle, make on the liquid absorbing arm to inhale and chew mouth and be in negative pressure state, waste liquid after the reaction in the incubation slot is sucked in the waste liquid bottle.In imbibition simultaneously, liquid-adding device starts execution next step liquid feeding work step.
After the present invention enters the bond step, displacement motor on the software control sliding mechanical, making it liquid adding arm on the sliding mechanical moves and aims on the shaking table on the 36 incubation slot setting slots, software control is set the liquid feeding digital pump and is rotated, and the liquid feeding digital pump quantitatively pumps the setting test solution in the P5 reagent bottle of setting, be transported on the liquid adding arm by connecting line and set liquid feeding in six passages and chew, add on the shaking table and set in the incubation slot, add test solution, the liquid feeding digital pump stops operating.Software continues control sliding mechanical superior displacement motor, make it liquid adding arm on the sliding mechanical and move to and hatch on next groove position of dish, software is controlled the liquid feeding digital pump again and is rotated, and the liquid feeding digital pump is in the P5 reagent bottle of setting, quantitatively pump the setting test solution, add shaking table and hatch in next groove of dish.So add test solution, till setting the N groove in addition.Shaking table wave hatch after, displacement motor on the software control sliding mechanical, making it liquid absorbing arm on the sliding mechanical moves and aims on the shaking table in the 36 incubation slot setting slots, start negative pressure pump simultaneously, make to be attached thereto and set up negative pressure in the waste liquid bottle, make on the liquid absorbing arm to inhale and chew mouth and be in negative pressure state, waste liquid after the reaction in the incubation slot is sucked in the waste liquid bottle.In imbibition simultaneously, liquid-adding device starts execution next step liquid feeding work step.
After the present invention enters the substrate step, displacement motor on the software control sliding mechanical, making it liquid adding arm on the sliding mechanical moves and aims on the shaking table on the 36 incubation slot setting slots, software control is set the liquid feeding digital pump and is rotated, and the liquid feeding digital pump quantitatively pumps the setting test solution in the P3 reagent bottle of setting, be transported in last six passages of liquid adding arm by connecting line and set liquid feeding and chew, add on the shaking table and set in the incubation slot, add test solution, the liquid feeding digital pump stops operating.Software continues control sliding mechanical superior displacement motor, make it liquid adding arm on the sliding mechanical and move to and hatch on next groove position of dish, software is controlled the liquid feeding digital pump again and is rotated, and the liquid feeding digital pump is in the P3 reagent bottle of setting, quantitatively pump the setting test solution, add shaking table and hatch in next groove of dish.So add test solution, till setting the N groove in addition.Shaking table wave hatch after, displacement motor on the software control sliding mechanical, making it liquid absorbing arm on the sliding mechanical moves and aims on the shaking table in the 36 incubation slot setting slots, start negative pressure pump simultaneously, make to be attached thereto and set up negative pressure in the waste liquid bottle, make on the liquid absorbing arm to inhale and chew mouth and be in negative pressure state, waste liquid after the reaction in the incubation slot is sucked in the waste liquid bottle.In imbibition simultaneously, liquid-adding device starts execution next step liquid feeding work step.
After the present invention enters H2O (water) step, displacement motor on the software control sliding mechanical, making it liquid adding arm on the sliding mechanical moves and aims on the shaking table on the 36 incubation slot setting slots, software control is set the liquid feeding digital pump and is rotated, and the liquid feeding digital pump quantitatively pumps the setting test solution in the P2 reagent bottle of setting, be transported on the liquid adding arm by connecting line and set liquid feeding in six passages and chew, add on the shaking table and set in the incubation slot, add test solution, the liquid feeding digital pump stops operating.Software continues control sliding mechanical superior displacement motor, make it liquid adding arm on the sliding mechanical and move to and hatch on next groove position of dish, software is controlled the liquid feeding digital pump again and is rotated, and the liquid feeding digital pump is in the P2 reagent bottle of setting, quantitatively pump the setting test solution, add shaking table and hatch in next groove of dish.So add test solution, till setting the N groove in addition.Shaking table wave hatch after, displacement motor on the software control sliding mechanical, making it liquid absorbing arm on the sliding mechanical moves and aims on the shaking table in the 36 incubation slot setting slots, start negative pressure pump simultaneously, make to be attached thereto and set up negative pressure in the waste liquid bottle, make on the liquid absorbing arm to inhale and chew mouth and be in negative pressure state, waste liquid after the reaction in the incubation slot is sucked in the waste liquid bottle.According to work step in the H2O step of setting, can set cycle index and finish the prompting warning.
The present invention hatches one to cover the disposable frid of hatching on the shaking table, press the step of Western Blotting western blot analysis, editing protein engram analysis parameter request on analyser, the disposable ProBloot membrane bar of hatching in the frid adds various different reagent automatically on the shaking table to hatching by the liquid adding arm parts, quantitative liquid separating reagent is controlled by a plurality of liquid feeding digital pump, by the editing parameter requirement, respectively by adding quantitative WASH (washing) reagent by a plurality of liquid feeding digital pump follow procedures from reagent bottle, CONJ1 (enzyme conjugates 1conjugation) reagent, CONJ2 (enzyme conjugates 2) reagent, SUBST (substrate solution Substrate) reagent and DIH2O (distilled water), by the immersion-washing-Jia sample-sample to the ProBloot membrane bar hatch-enzyme combination-Jia substrate-biochemical reactions such as washing after, to the qualitative analysis of protein molecular.The present invention at most once can carry out the check and analysis simultaneously of 36 samples, and minimum interval 3S adds reagent and inhales waste liquid.Hatch shaking table and can select high, normal, basic three kinds of different shaking table swing speed, protect whole ProBloot membrane bar and in whole Western Blotting western blot analysis process, keep soaking, do not produce the dry film phenomenon, make the background of ProBloot membrane clear.The shaking table of hatching of the present invention provides four kinds of incubation temperature (room temperature, 25 ℃, 30 ℃, 37 ℃) to select.Liquid adding arm parts of the present invention can be provided with six reagent passage mouths at most.A plurality of liquid feeding digital pump of the present invention is made up of six digital pump, has digital quantitative liquid feeding selection function within the 50-3000uL.The present invention produces reaction back waste liquid and is handled by liquid absorbing arm parts and band warning negative pressure suction pumps in western blot analysis, waste liquid produces negative pressure by the liquid absorbing arm parts by the suction pumps parts and sucks in the totally-enclosed waste liquid bottle of P1, and waste liquid overflow warning device is arranged in the P1 waste liquid bottle.The present invention works out special software, and software function has project editor, operation automatically, pipeline cleans, calibrates, is provided with function automatically.The present invention can carry out 36 pattern detection analyses, suitable batch standardization western blot analysis simultaneously.
In sum, principal feature of the present invention comprises as follows:
(1) the present invention adopts digital pipe pump technology, test solution liquid feeding technology is easily controlled, accurately, liquid feeding is stepless from 50uL～3000uL numeral, can positive and negative suction controlling plumbing fixtures pump, be much better than existing application malleation pressure control test solution liquid feeding technology, malleation pressure control test solution liquid feeding technology is subject to pressure surge and disturbs, particularly stablize the restriction that is based upon air container shape size, seal degree and time and sensing element of malleation, these factors will influence the precision of test solution liquid feeding, and the control of prior art also needs solenoid valve.
(2) the present invention adopts digital pipe pump test solution liquid feeding technology, and its piping system easy cleaning is easily changed, and no dead angle is convenient to robotization and is cleaned.And existing malleation pressure control liquid feeding technique device also needs solenoid valve owing to there is pressure vessel to exist with its control, and easy cleaning more is not easy to robotization and cleans, and has the cleaning dead angle.
(3) liquid adding arm of the present invention and liquid absorbing arm are installed on the sliding mechanical device, under software control, can be to any groove position liquid feeding and the imbibition of 1 on the shaking table～36 incubation slots, and suitable automatic batch is disposed sample simultaneously.And the liquid adding arm of prior art and liquid absorbing arm are installed on the fixed position, only are suitable for single sample disposal.
(4) shaking table technology of the present invention adopts eccentric wheel and controllable motor structure, can provide 3 kinds to wave rate selection, and the shaking table temperature control technology adopts the heating of low-voltage safety film, and 3 kinds of temperature control temperature are provided, and adapts to need of work.Prior art adopts resistance wire and technology such as damp and hot heating usually, and volume is big, and temperature control is difficult for.Wave speed single-rate, adaptability deficiency generally only are provided.
(5) the present invention adopts novel imbibition to chew structure, and this novel imbibition is chewed structure in the imbibition process, and the experimental film bar that is in groove is never adsorbed, and only inhales test solution, and in whole test, cross pollution is close to 0 between each sample, and is safe and reliable.Prior art is in imbibition, and for the experimental film bar of light weight, its possibility that is adsorbed is about 1-10%.To make indivedual sample test failures, cause cross pollution between sample.
(6) dish is hatched in the present invention's disposable use that 1 16 grooved put in new design on shaking table, workbench for steps such as the hatching of Western blot sample, washing lotion, combination, substrates, the reliability of cross pollution and Western blot sample between sample is avoided in disposable use.This is different from the reliability that prior art is reused cross pollution and detection between the sample bring.
Though more than described the specific embodiment of the present invention, it will be understood by those of skill in the art that these only illustrate, under the prerequisite that does not deviate from principle of the present invention and essence, can make numerous variations or modification to these embodiments.Therefore, protection scope of the present invention is limited by appended claims.
Claims (4)
1. automatic westem blot analyzer, it is characterized in that, it comprises hatches shaking table, liquid adding arm parts, displacement motor, liquid absorbing arm parts, imbibition motor, band warning negative pressure suction pumps and a plurality of liquid feeding digital pump, hatch shaking table and have a plurality of incubation slots, liquid adding arm parts and liquid absorbing arm parts are installed on the sliding mechanical device and respectively the incubation slot of hatching in the shaking table are added test solution and inhales waste liquid, the liquid adding arm parts are connected with displacement motor, liquid feeding digital pump, and the liquid absorbing arm parts are connected with imbibition motor, band warning negative pressure suction pumps.
2. automatic westem blot analyzer as claimed in claim 1 is characterized in that described automatic westem blot analyzer also comprises a cage, and this cage covers shaking table parts, liquid adding arm parts, liquid absorbing arm parts and sliding mechanical device fully.
3. automatic westem blot analyzer as claimed in claim 1 is characterized in that, described liquid feeding digital pump is connected with a bottle for handling liquid toilet or cosmetic substance or a reagent bottle.
4. automatic westem blot analyzer as claimed in claim 1 is characterized in that, described liquid adding arm parts comprise that liquid feeding chews and the liquid feeding sebific duct, and liquid feeding is chewed an end that connects the liquid feeding sebific duct, and the other end of liquid feeding sebific duct is connected with digital fluid filling pump.
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| CN 200910052971 CN101923096B (en) | 2009-06-12 | 2009-06-12 | Automatic westem blot analyzer |
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| CN 200910052971 CN101923096B (en) | 2009-06-12 | 2009-06-12 | Automatic westem blot analyzer |
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| CN105628936A (en) * | 2016-03-22 | 2016-06-01 | 苏州捷美电子有限公司 | Western Blot laboratory instrument |
| CN106733886A (en) * | 2016-12-20 | 2017-05-31 | 上海长海医院 | A kind of full automatic high efficiency western blot wash membranous system |
| EP3218112A4 (en) * | 2014-11-14 | 2018-05-09 | Cytoskeleton, Inc. | Modular fluid dispensing devices |
| CN109187990A (en) * | 2018-08-30 | 2019-01-11 | 莫纳（苏州）生物科技有限公司 | A kind of immunoblotting assay instrument and control system |
| US10288636B2 (en) | 2014-11-14 | 2019-05-14 | Cytoskeleton, Inc. | Modular fluid dispensing devices |
| CN110514500A (en) * | 2019-09-24 | 2019-11-29 | 江苏中朗宏泰科学仪器有限公司 | A kind of high throughput automatic sample pre-treatment instrument, application and application method |
| CN112666354A (en) * | 2020-12-15 | 2021-04-16 | 韦克斯科技(北京)有限公司 | Full-automatic western blot processing system |
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| CN2859530Y (en) * | 2005-12-29 | 2007-01-17 | 王维锋 | Protein mark immunological automatic detection |
| CN201094106Y (en) * | 2007-08-28 | 2008-07-30 | 东南大学 | Automatic egg albumen immune print eluting instrument |
| CN101153874A (en) * | 2007-08-28 | 2008-04-02 | 东南大学 | Automatic protein hybridization elution instrument |
| CN201417275Y (en) * | 2009-06-12 | 2010-03-03 | 上海迅达医疗仪器有限公司 | Automatic Western blot analyzer |
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2009
- 2009-06-12 CN CN 200910052971 patent/CN101923096B/en not_active Expired - Fee Related
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| EP3218112A4 (en) * | 2014-11-14 | 2018-05-09 | Cytoskeleton, Inc. | Modular fluid dispensing devices |
| US10288636B2 (en) | 2014-11-14 | 2019-05-14 | Cytoskeleton, Inc. | Modular fluid dispensing devices |
| CN105628936A (en) * | 2016-03-22 | 2016-06-01 | 苏州捷美电子有限公司 | Western Blot laboratory instrument |
| CN106733886A (en) * | 2016-12-20 | 2017-05-31 | 上海长海医院 | A kind of full automatic high efficiency western blot wash membranous system |
| CN109187990A (en) * | 2018-08-30 | 2019-01-11 | 莫纳（苏州）生物科技有限公司 | A kind of immunoblotting assay instrument and control system |
| CN110514500A (en) * | 2019-09-24 | 2019-11-29 | 江苏中朗宏泰科学仪器有限公司 | A kind of high throughput automatic sample pre-treatment instrument, application and application method |
| CN112666354A (en) * | 2020-12-15 | 2021-04-16 | 韦克斯科技(北京)有限公司 | Full-automatic western blot processing system |
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| CN101923096B (en) | 2013-01-02 |
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