CN2859530Y - Protein mark immunological automatic detection - Google Patents
Protein mark immunological automatic detection Download PDFInfo
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- CN2859530Y CN2859530Y CN 200520048182 CN200520048182U CN2859530Y CN 2859530 Y CN2859530 Y CN 2859530Y CN 200520048182 CN200520048182 CN 200520048182 CN 200520048182 U CN200520048182 U CN 200520048182U CN 2859530 Y CN2859530 Y CN 2859530Y
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- liquid storage
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- reactive tank
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Abstract
The utility model discloses a protein mark immunological automatic detector. The device can automatically complete the change of the reagent in the immunological detecting portion of the protein mark experiment, which reduces the operator's working strength and saves working time. The device comprises a single-chip, a micropump, an electromagnetic valve, a liquid store bottle, a reaction groove and pipelines. The micropump comprises a booster pump and a vacuum pump. The electromagnetic valve comprises a group of verse electromagnetic valves and a group of reverse electromagnetic valves. The verse electromagnetism valves connect with the booster pump. The liquid store bottle comprises at least two enclosed liquid store bottles and the pipelines comprise windpipes and liquid pipes. The windpipes connect with the liquid store pump, the electromagnetic valve as well as the electromagnetic valve and the micropump. The liquid pipes are used to connect the reaction groove with the liquid store bottle. The singlechip controls the actions of the electromagnetic valve and the micropump which connects with the liquid store groove through the electromagnetic valve. The solid phase support adhered by the target protein is mounted in the reaction groove.
Description
Technical field
The utility model relates to a kind of protein immunoblot experiment device, relates in particular to a kind of Western blot immunity automatic tester.
Background technology
Detected by Western blot is that the protein component of electrophoretic separation is transferred to a kind of solid support from gel, detect by antibody and the epitope generation specific immune response that target protein presented that is attached to solid support, whole process comprises: protein electrophorese separates, electrotransfer, immunology detection and input four parts.At present, immune detection part in the known protein blot experiment comprises following process: sealing, and the first antibody combination is cleaned, and the second antibody combination is cleaned.The reagent that needs to handle in this part experiment flow is confining liquid, first antibody dilution, cleaning buffer solution, second antibody dilution, cleaning buffer solution.What adopt at present is that manual method is changed different reagent.Frequent change the liquid operation and increased experimenter's working strength and lose time.
The utility model content
Technical problem to be solved in the utility model provides a kind of Western blot immunity automatic tester, and this instrument can automatically be finished the reagent of immune detection part in the protein blot experiment and change, and can reduce operator's working strength and save time.
In order to solve above technical matters, the utility model provides a kind of Western blot immunity automatic tester, wherein, it is by single-chip microcomputer, micropump, solenoid valve, liquid storage bottle, reactive tank and pipeline constitute, described micropump comprises supercharge pump and vacuum pump, described solenoid valve comprises one group of forward solenoid valve and one group of reverse solenoid valve, described forward solenoid valve links to each other with supercharge pump, described reverse solenoid valve links to each other with vacuum pump, described liquid storage bottle comprises plural sealing liquid storage bottle, described pipeline comprises tracheae and liquid pipe, tracheae connects liquid storage pump and solenoid valve and solenoid valve and micropump, the liquid pipe is used for coupled reaction groove and liquid storage bottle, described single-chip microcomputer is by the action of control electromagnetic valve and micropump, and micropump links to each other with reservoir by solenoid valve, and the solid support that adheres to target protein is housed in the described reactive tank, also comprise a shaking table under the described reactive tank, described reactive tank shakes and shakes with shaking table.
Described reactive tank and or liquid storage bottle dispose temperature control equipment.
Because the utility model adopts the Single-chip Controlling solenoid valve, and then the control supercharge pump and the vacuum pump that link to each other with solenoid valve, pressurization and depressurization by supercharge pump and vacuum pump, the liquid that can control liquid storage bottle flow in the reactive tank with the target protein reaction or cleans, and the liquid after using can be drawn in the liquid storage bottle, so just can finish the work of the part of immune detection in the protein blot experiment automatically, so, time of operator's working strength and saving can be reduced.Because the effect of shaking table is arranged, can make reaction more even in addition, make the reagent in the reactive tank reach abundant mixing purpose, and can monitor the temperature of reactive tank and liquid storage bottle by temperature control equipment.
Description of drawings
Below in conjunction with the drawings and specific embodiments the utility model is further elaborated.
Accompanying drawing is a control synoptic diagram of the present utility model.
Embodiment
Accompanying drawing is a control synoptic diagram of the present utility model.It is made of single-chip microcomputer, micropump, solenoid valve, liquid storage bottle, reactive tank, shaking table and pipeline, wherein, micropump comprises supercharge pump and vacuum pump, supercharge pump and vacuum pump can or be split for one, liquid storage bottle comprises waste liquid liquid storage bottle, first antibody dilution liquid storage bottle, second antibody dilution liquid storage bottle, cleaning fluid liquid storage bottle, confining liquid liquid storage bottle, single-chip microcomputer has several control mouths to link to each other with solenoid valve and pump, by the built-in experiment flow module controls solenoid valve and the action of pump.This module is divided into several separate steps, and each step can be finished one and change the liquid operation, and the user can be to the time of each step, and the volume and the multiplicity of agents useful for same are set.Solenoid valve is divided into two groups: one group is that (forward is the direction of air-flow from the pump to the liquid storage bottle to the forward solenoid valve, shown in reference arrow), can reach the purpose that the liquid storage bottle internal pressure is raise, it also comprises an air release, is used to reach the purpose of balance liquid storage bottle internal gas pressure and ambient atmosphere pressure balance; Another group (is reversed the direction of air-flow from the liquid storage bottle to the pump for reverse solenoid valve, shown in reference arrow), can reach the purpose that the liquid storage bottle internal pressure is reduced, it also comprises an air release, is used to reach the purpose of balance liquid storage bottle internal gas pressure and ambient atmosphere pressure balance.Supercharge pump (can pressure boost) links to each other with one group of forward solenoid valve by several joints and plastic tube, and vacuum pump (can reduce pressure) passes through several joints and plastic tube links to each other with one group of reverse solenoid valve.Liquid storage bottle is the good plastic bottle of sealing, bores two holes thereon, all is positioned on the liquid level.One of them links to each other with solenoid valve by joint and plastic tube, reaches the purpose that air-flow is entered or leave liquid storage bottle.Another links to each other with reactive tank by joint and plastic tube, reaches to make liquid in the liquid storage bottle be pressed into reactive tank or make liquid in the reactive tank suck the purpose of liquid storage bottle.Reactive tank is a without leakage container, links to each other with each liquid storage bottle by some joints and plastic tube.Reactive tank places on the shaking table, and shaking table can make the reagent in the reactive tank reach abundant mixing purpose.
After beginning to seal step, the Single-chip Controlling supercharge pump starts opens the forward solenoid valve that links to each other with the confining liquid liquid storage bottle simultaneously, pressure in this liquid storage bottle is raise, thereby confining liquid is pressed into the reactive tank that is arranged on the shaking table, after extruding the confining liquid of certain volume, supercharge pump quits work, but the state that the maintenance of the forward solenoid valve that links to each other with the confining liquid liquid storage bottle is opened, opening air release in the forward solenoid valve simultaneously makes this pressure of the inside of a bottle identical with ambient atmosphere pressure and stop to extrude reagent, utilize shaking table adequate closure a period of time (can be set by the user), after reaching setting-up time, the Single-chip Controlling vacuum pump starts opens the reverse solenoid valve that links to each other with the waste liquid liquid storage bottle simultaneously, make this bottle be in the negative pressure state, thereby the confining liquid in the reactive tank is sucked the waste liquid liquid storage bottle, and after suction finished, vacuum pump quit work, the reverse solenoid valve that links to each other with the waste liquid liquid storage bottle keeps the state opened, opens air release in the reverse solenoid valve simultaneously and makes this pressure of the inside of a bottle identical with ambient atmosphere pressure and stop to suck reagent.
After the beginning first antibody integrating step, the forward solenoid valve that links to each other with first antibody dilution liquid storage bottle is opened in the startup of Single-chip Controlling supercharge pump simultaneously, and (forward is the direction of air-flow from the pump to the liquid storage bottle, shown in reference arrow), pressure in the first antibody dilution liquid storage bottle is raise, thereby dilution is pressed into the reactive tank that is arranged on the shaking table, after extruding the first antibody dilution of certain volume, air pump quits work but state that the maintenance of the forward solenoid valve that links to each other with first antibody dilution liquid storage bottle is opened is opened air release simultaneously and made this pressure of the inside of a bottle identical with ambient atmosphere pressure and stop to extrude reagent, utilize shaking table fully in conjunction with a period of time (can be set by the user), after reaching setting-up time, the startup of Single-chip Controlling vacuum pump is opened the reverse solenoid valve that links to each other with first antibody dilution liquid storage bottle simultaneously and (is reversed the direction of air-flow from the liquid storage bottle to the pump, shown in reference arrow), make this bottle be in the negative pressure state, thereby the first antibody dilution is sucked first antibody dilution liquid storage bottle.After suction finished, vacuum pump quit work, and the reverse solenoid valve that links to each other with first antibody dilution liquid storage bottle keeps the state opened, opened air release in the reverse solenoid valve simultaneously and made this pressure of the inside of a bottle identical with ambient atmosphere pressure and stop to suck reagent.
Behind the beginning cleaning step, the Single-chip Controlling supercharge pump starts opens the forward solenoid valve that links to each other with the cleaning fluid liquid storage bottle simultaneously, pressure in this liquid storage bottle is raise, thereby cleaning fluid is pressed into the reactive tank that is arranged on the shaking table, after extruding the cleaning fluid of certain volume, supercharge pump quits work but the forward solenoid valve that links to each other with the cleaning fluid liquid storage bottle keeps the state opened, opening air release in the forward solenoid valve simultaneously makes this pressure of the inside of a bottle identical with ambient atmosphere pressure and stop to extrude reagent, utilize shaking table fully to clean a period of time (can be set by the user), after reaching setting-up time, the Single-chip Controlling vacuum pump starts opens the solenoid valve that links to each other with the waste liquid liquid storage bottle simultaneously, make this bottle be in the negative pressure state, thereby cleaning fluid is sucked the waste liquid liquid storage bottle.After suction finished, vacuum pump quit work, and the reverse solenoid valve that links to each other with the waste liquid liquid storage bottle keeps the state opened, opened air release in the reverse solenoid valve simultaneously and made this pressure of the inside of a bottle identical with ambient atmosphere pressure and stop to suck reagent.The user can set cycle index in this step.
After the beginning second antibody integrating step, the forward solenoid valve that links to each other with second antibody dilution liquid storage bottle is opened in the startup of Single-chip Controlling supercharge pump simultaneously, and (forward is the direction of air-flow from the pump to the liquid storage bottle, shown in reference arrow), pressure in this liquid storage bottle is raise, thereby dilution is pressed into the reactive tank that is arranged on the shaking table, after extruding the second antibody dilution of certain volume, air pump quits work, but the state that the forward solenoid valve that links to each other with second antibody dilution liquid storage bottle maintenance is opened, opening air release in the forward solenoid valve simultaneously makes this pressure of the inside of a bottle identical with ambient atmosphere pressure and stop to extrude reagent, utilize shaking table fully in conjunction with a period of time (can be set by the user), after reaching setting-up time, the startup of Single-chip Controlling micropump is opened the reverse solenoid valve that links to each other with second antibody dilution liquid storage bottle simultaneously and (is reversed the direction of air-flow from the liquid storage bottle to the pump, shown in reference arrow), make this bottle be in the negative pressure state, thereby the second antibody dilution is sucked second antibody dilution liquid storage bottle.After suction finished, vacuum pump quit work, and the reverse solenoid valve that links to each other with second antibody dilution liquid storage bottle keeps the state opened, opened air release in the reverse solenoid valve simultaneously and made this pressure of the inside of a bottle identical with ambient atmosphere pressure and stop to suck reagent.
In addition, the utility model is equipped with the temperature control equipment (not shown) on reactive tank and/or liquid storage bottle, it can be installed in position and go up, bottom, middle part or top such as reactive tank and/or liquid storage bottle, this can set as required, and temperature control equipment can be the more common accessory of existing market, can or carry control system by Single-chip Controlling of the present utility model.
More than be exactly workflow of the present utility model, certainly increase liquid storage bottle as required, but its workflow be similar to the above, repeats no more.
Claims (10)
1, a kind of Western blot immunity automatic tester, it is characterized in that, it is by single-chip microcomputer, micropump, solenoid valve, liquid storage bottle, reactive tank and pipeline constitute, described micropump comprises supercharge pump and vacuum pump, described solenoid valve comprises one group of forward solenoid valve and one group of reverse solenoid valve, described forward solenoid valve links to each other with supercharge pump, described reverse solenoid valve links to each other with vacuum pump, described liquid storage bottle comprises plural sealing liquid storage bottle, described pipeline comprises tracheae and liquid pipe, tracheae connects liquid storage pump and solenoid valve and solenoid valve and micropump, the liquid pipe is used for coupled reaction groove and liquid storage bottle, the action of described Single-chip Controlling solenoid valve and micropump, micropump links to each other with reservoir by solenoid valve, the solid support that adheres to target protein is housed in the described reactive tank, also comprises a shaking table under the described reactive tank, described reactive tank shakes and shakes with shaking table.
2, Western blot as claimed in claim 1 immunity automatic tester is characterized in that, described reactive tank and or liquid storage bottle dispose temperature control equipment.
3, Western blot immunity automatic tester as claimed in claim 1 or 2 is characterized in that one of them of described sealing liquid storage bottle is the waste liquid liquid storage bottle, and it links to each other with reactive tank with reverse solenoid valve respectively by pipeline.
4, Western blot immunity automatic tester as claimed in claim 1 or 2 is characterized in that one of them of described sealing liquid storage bottle is the confining liquid liquid storage bottle, and it links to each other with reactive tank with the forward solenoid valve respectively by pipeline.
5, Western blot immunity automatic tester as claimed in claim 3 is characterized in that one of them of described sealing liquid storage bottle is the confining liquid liquid storage bottle, and it links to each other with reactive tank with the forward solenoid valve respectively by pipeline.
6, Western blot immunity automatic tester as claimed in claim 1 or 2 is characterized in that one of them of described sealing liquid storage bottle is the cleaning fluid liquid storage bottle, and it links to each other with reactive tank with the forward solenoid valve respectively by pipeline.
7, Western blot immunity automatic tester as claimed in claim 3 is characterized in that one of them of described sealing liquid storage bottle is the cleaning fluid liquid storage bottle, and it links to each other with reactive tank with the forward solenoid valve respectively by pipeline.
8, as claim 1 or 2 or 5 or 7 described Western blot immunity automatic testers, it is characterized in that, one of them of described sealing liquid storage bottle is the antibody diluent liquid storage bottle, a port of described antibody diluent reservoir links to each other with reactive tank by pipeline, and the another port is with described forward solenoid valve and oppositely solenoid valve is in parallel.
9, Western blot immunity automatic tester as claimed in claim 3, it is characterized in that, one of them of described sealing liquid storage bottle is the antibody diluent liquid storage bottle, a port of described antibody diluent reservoir links to each other with reactive tank by pipeline, and the another port is with described forward solenoid valve and oppositely solenoid valve is in parallel.
10, Western blot immunity automatic tester as claimed in claim 4, it is characterized in that, one of them of described sealing liquid storage bottle is the antibody diluent liquid storage bottle, a port of described antibody diluent reservoir links to each other with reactive tank by pipeline, and the another port is with described forward solenoid valve and oppositely solenoid valve is in parallel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520048182 CN2859530Y (en) | 2005-12-29 | 2005-12-29 | Protein mark immunological automatic detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520048182 CN2859530Y (en) | 2005-12-29 | 2005-12-29 | Protein mark immunological automatic detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2859530Y true CN2859530Y (en) | 2007-01-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520048182 Expired - Fee Related CN2859530Y (en) | 2005-12-29 | 2005-12-29 | Protein mark immunological automatic detection |
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| Country | Link |
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| CN (1) | CN2859530Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101833007A (en) * | 2010-02-25 | 2010-09-15 | 上海迅达医疗仪器有限公司 | Protein blot detection device |
| CN101923096B (en) * | 2009-06-12 | 2013-01-02 | 上海迅达医疗仪器有限公司 | Automatic westem blot analyzer |
| CN103513024A (en) * | 2012-06-20 | 2014-01-15 | 上海市东方医院 | Full-automatic immunochemical instrument and use method thereof |
| CN105628936A (en) * | 2016-03-22 | 2016-06-01 | 苏州捷美电子有限公司 | Western Blot laboratory instrument |
| CN110361538A (en) * | 2019-07-08 | 2019-10-22 | 西安交通大学 | It is a kind of can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application |
-
2005
- 2005-12-29 CN CN 200520048182 patent/CN2859530Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101923096B (en) * | 2009-06-12 | 2013-01-02 | 上海迅达医疗仪器有限公司 | Automatic westem blot analyzer |
| CN101833007A (en) * | 2010-02-25 | 2010-09-15 | 上海迅达医疗仪器有限公司 | Protein blot detection device |
| CN101833007B (en) * | 2010-02-25 | 2013-04-03 | 上海迅达医疗仪器有限公司 | Protein blot detection device |
| CN103513024A (en) * | 2012-06-20 | 2014-01-15 | 上海市东方医院 | Full-automatic immunochemical instrument and use method thereof |
| CN103513024B (en) * | 2012-06-20 | 2015-08-26 | 上海市东方医院 | Full-automatic immunochemical instrument and using method thereof |
| CN105628936A (en) * | 2016-03-22 | 2016-06-01 | 苏州捷美电子有限公司 | Western Blot laboratory instrument |
| CN110361538A (en) * | 2019-07-08 | 2019-10-22 | 西安交通大学 | It is a kind of can the full-automatic western antibody incubation Film washer that remotely controls of cell phone application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070117 Termination date: 20101229 |