CN103513024A - Full-automatic immunochemical instrument and use method thereof - Google Patents
Full-automatic immunochemical instrument and use method thereof Download PDFInfo
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- CN103513024A CN103513024A CN201210206355.7A CN201210206355A CN103513024A CN 103513024 A CN103513024 A CN 103513024A CN 201210206355 A CN201210206355 A CN 201210206355A CN 103513024 A CN103513024 A CN 103513024A
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- 238000000034 method Methods 0.000 title claims abstract description 50
- 230000000984 immunochemical effect Effects 0.000 title abstract description 4
- 239000007788 liquid Substances 0.000 claims abstract description 95
- 238000006243 chemical reaction Methods 0.000 claims abstract description 71
- 239000002699 waste material Substances 0.000 claims abstract description 42
- 239000011159 matrix material Substances 0.000 claims abstract description 9
- 239000012530 fluid Substances 0.000 claims description 34
- 239000000376 reactant Substances 0.000 claims description 19
- 239000003153 chemical reaction reagent Substances 0.000 claims description 17
- 239000006210 lotion Substances 0.000 claims description 14
- 238000005406 washing Methods 0.000 claims description 14
- 238000012545 processing Methods 0.000 claims description 8
- 239000010808 liquid waste Substances 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 4
- 230000005611 electricity Effects 0.000 claims description 4
- 230000008569 process Effects 0.000 abstract description 22
- 230000006378 damage Effects 0.000 abstract description 13
- 238000011160 research Methods 0.000 abstract description 2
- 208000027418 Wounds and injury Diseases 0.000 abstract 1
- 208000014674 injury Diseases 0.000 abstract 1
- 231100000614 poison Toxicity 0.000 abstract 1
- 239000003440 toxic substance Substances 0.000 abstract 1
- 210000004748 cultured cell Anatomy 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 7
- 238000005213 imbibition Methods 0.000 description 7
- SXEHKFHPFVVDIR-UHFFFAOYSA-N [4-(4-hydrazinylphenyl)phenyl]hydrazine Chemical compound C1=CC(NN)=CC=C1C1=CC=C(NN)C=C1 SXEHKFHPFVVDIR-UHFFFAOYSA-N 0.000 description 6
- 238000006073 displacement reaction Methods 0.000 description 5
- 238000007667 floating Methods 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 230000004907 flux Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000001473 noxious effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 238000003364 immunohistochemistry Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000003118 histopathologic effect Effects 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 231100000004 severe toxicity Toxicity 0.000 description 1
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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Abstract
The invention relates to the fields of life science and medical research, and discloses a full-automatic immunochemical instrument and a use method thereof. According to the invention, the full-automatic immunochemical instrument can realize automatic liquid charging and waste liquid suction in the whole process. By means of changing the liquid without changing a vessel hole, damage or position shift to a tissue slice sample due to frequent movement of the sample can be avoided in an operation process. Also, experimenters can avoid suffer body injury caused by direct contact with toxic substances. By automatic instrument operation, the labor intensity is alleviated, the efficiency is improved, artificial error can be avoided, and automation and standardization can be realized. A liquid distribution and liquid discharge structure and a liquid pipeline can move up and down, can drop during liquid charging and liquid suction, and rise during reaction vessel change, so that the operation can be more convenient. The liquid distribution and liquid discharge structure and the liquid pipeline can move around, batch treatment can be carried out on a reaction vessel hole matrix, and the efficiency can be higher.
Description
Technical field
The present invention relates to life science and medical research field, the full-automatic immunocytochemical technique of the particularly full-automatic immunohistochemistry technology of histopathologic slide, and cultured cell.
Background technology
Immunochemistry dyeing comprises immunohistochemistry and immunocytochemistry, it is laboratory work classical, conventional and that requirement is very rigorous, immunochemistry changes very responsive for the albumen of tissue, cell, can locate, again can sxemiquantitative, and be a kind of very valuable detection technique means.Expression, with regard to the existence form of histotomy, mainly contains two kinds of modes: paster method and floating (floating) sheet method.Floating sheet method refers to that histotomy is floating, is free in liquid and hatches, react, and waits the process of hatching to finish and reacts after quality, then histotomy is mounted on glass sheet.The benefit of this method is exactly for compared with slab (can observe body compared with maxicell overall picture or structure), can incubation reaction obtain comparatively thorough, fully, completely, true, be that paster method can not be compared.
At present, paster method has been realized robotization, but floating sheet method and with the immunocytochemical technique of double dish or culture plate cultured cell is still manual operation.Artificial behaviour is wasted time and energy, operate miss, experiment parameter variation etc., easily causes experimental result unstable.
The present inventor's discovery, problem is in the following areas deposited in manual operations:
1, time-consuming taking a lot of work: immunochemistry operating process each time at least needs 7 to 8 hours consuming time, and even more than 1 to 2 day, step is many, dry as dust; Each experiment can only be processed finite sample, as 5 to 7 examples;
2, easily make mistakes: immunochemistry operates nearly tens kinds of antibody used and reagent type each time, manual operations easily mistake adds or forgets and adds, and the reaction time can not accurately be controlled with standard and hold;
3, between different experiments batch, due to multifactorial impact, be difficult to realize and reappear completely and matching, differences between batches are difficult to prevent.
4, histotomy sample easily damages: in manual process, need frequent moving tissue section sample, easily it is caused to damage, and easily with dragging for into pulling action out, cause sample displacement;
5, damage healthy: immunochemistry operates antibody used and reagent severe toxicity a bit, and if diaminobenzidine (DAB) is clear and definite strong carcinogen, in manual process, experimenter directly contacts harmful liquid, damages healthy.
Therefore, need badly now and a kind ofly can realize overall process automatic operation, neither can damage, displacement histotomy sample, can not damage the immunochemical technique healthy, objective, efficient, flux is large and pertinent instruments equipment because of direct contact harmful liquid yet.
Summary of the invention
The object of the present invention is to provide a kind of full-automatic immunochemistry instrument and using method thereof, can avoid damage, displacement histotomy sample in operating process, and, also can avoid experimenter directly contact harmful liquid and health is damaged, simultaneously, the reliability, repeatability and the standardization that improve experimental result, realize large flux, high-level efficiency.
For solving the problems of the technologies described above, embodiments of the present invention disclose a kind of full-automatic immunochemistry instrument, comprising: at least three reagent bottles (2), liquid distribute and liquid discharge structure (3), at least one fluid pipeline (4) and at least one porous reaction ware (5).
Reagent bottle (2) is for holding reactant liquor, washing lotion and liquid waste processing liquid;
Liquid distributes and liquid discharge structure (3) is connected between reagent bottle (2) and fluid pipeline (4), for the liquid of reagent bottle (2) is injected in reaction utensil (5) by fluid pipeline (4), or the waste liquid in reaction utensil (5) is passed through to fluid pipeline (4) sucking-off.
Embodiments of the present invention also disclose a kind of using method of full-automatic immunochemistry instrument, and the full-automatic immunochemistry instrument for as described above, comprises the following steps:
Reactant liquor is joined in reaction utensil, fully after reaction, by waste liquid sucking-off from reaction utensil;
Washing lotion is joined in reaction utensil, after fully cleaning, by waste liquid sucking-off from reaction utensil;
If immunochemistry operation does not all complete yet, next reactant liquor is joined in reaction utensil.
Compared with prior art, the key distinction and effect thereof are embodiment of the present invention:
Compare with pure manual immunochemistry operating process in prior art, this full-automatic immunochemistry instrument can be realized overall process automatic liquid-feeding and sucking-off waste liquid, by changing the mode in Ye Buhuanmin hole, can avoid frequent moving tissue section sample in operating process and it is caused to damage, prevent the displacement of sample between different wares hole, avoid experimenter directly to contact noxious material and health is damaged, improving conventional efficient, increase experiment flux, reduce experimental error.
Further, liquid distributes with liquid discharge structure and fluid pipeline and can move up and down, and when liquid feeding and imbibition, reduces, and when changing reaction utensil, raises, and makes more convenient to operate.
Further, liquid distributes with liquid discharge structure and fluid pipeline and can all around move, and can realize batch processing to sample matrixes such as histotomy or cultured cells, and efficiency is higher.
Further, in general, the toxicity of waste liquid is all very strong, very large to the harm of human body and physical environment, by waste collection and harmless treating apparatus, waste liquid is carried out, after harmless treatment, can realizing qualified discharge, safety and environmental protection.
Further, the environment of 4 degrees Celsius of constant temperature and lucifuge.4 degrees Celsius of constant temperature, can guarantee variously to prepare stand-by hydraulic fluid in a kind of stable state, extend the expiration date; Under the constant condition of this kind of low temperature, can make speed that immune response carries out slowly, in conjunction with special, to reduce background local; Can guarantee that this full-automatic immunochemistry instrument, under any natural conditions, can work for a long time, reactant liquor and sample can keep optimum performance.The environment of lucifuge, is in order to meet when carrying out Immunofluorescence Reactions, the requirement of fluorescent reagent to light.
Further, the albumen in histotomy sample self is electronegative, by reaction utensil is added after positive electricity, and positive and negative attracting each other, thus guarantee that histotomy sample can not be sucked away when sucking-off waste liquid.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of full-automatic immunochemistry instrument in first embodiment of the invention;
Fig. 2 is the schematic flow sheet of a kind of using method of full-automatic immunochemistry instrument in second embodiment of the invention.
Embodiment
In the following description, in order to make reader understand the application better, many ins and outs have been proposed.But, persons of ordinary skill in the art may appreciate that even without these ins and outs and the many variations based on following embodiment and modification, also can realize each claim of the application technical scheme required for protection.
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with accompanying drawing, embodiments of the present invention are described in further detail.
First embodiment of the invention relates to a kind of full-automatic immunochemistry instrument.Fig. 1 is the structural representation of this full-automatic immunochemistry instrument.
Specifically, as shown in Figure 1: this full-automatic immunochemistry instrument comprises: at least three reagent bottles 2, liquid distribute and liquid discharge structure 3, at least one fluid pipeline 4 and at least one porous reaction ware 5.
At least three reagent bottles 2, one of them is used for holding washing lotion, other be used for holding various reactant liquors used in immunochemistry operating process, specifically, various reactant liquors comprise: various antibody is as various primary antibodies, various two anti-, three anti-, and positive, negative controls, reaction substrate---diaminobenzidine (DAB), hydrogen peroxide etc.; Washing lotion is cleaned for samples such as the histotomy to after reactant liquor reaction or cultured cells, and in practical operation, modal washing lotion is exactly phosphate buffer, also can comprise in addition the washing lotion of other kind.
Liquid distributes and liquid discharge structure 3 is connected between reagent bottle 2 and fluid pipeline 4, for the liquid of reagent bottle 2 is injected in reaction utensil 5 by fluid pipeline 4, or the waste liquid in reaction utensil 5 is passed through to fluid pipeline 4 sucking-offs.In operating process, liquid distributes and liquid discharge structure 3 utilizes motor or other power, realizes the operation of accurate liquid feeding or drawing liquid.Whole operating process is by programmed control, and program can be write.
As shown in Figure 1, each reacting hole of reaction utensil 5 is that oral area is circular groove shapes, and mouth diameters is 1 centimetre to 10 centimetres, and reaction utensil can be that bottom and the loins in special ，Min hole is mounted with electrode; Also can use the culture plates such as 6 ,24 holes, ,12 hole, hole or the double dish of standard to replace, thereby make the adaptability of this instrument stronger, better compatible.
Between a plurality of reacting holes of reaction utensil 5, be matrix form and arrange, the line space of this matrix equates with the distance of 4 of each fluid pipelines.
When reaction utensil 5 has when a plurality of, between them, be also matrix form and arrange.
Liquid distributes with liquid discharge structure 3 and fluid pipeline 4 and can move up and down, and when liquid feeding and imbibition, reduces, and when changing reaction utensil, raises, and makes more convenient to operate.
As shown in Figure 1, liquid distribution is fixed together with liquid discharge structure 3 and fluid pipeline 4, and both can move together.By movement, the imbibition mouth of pipe of fluid pipeline 4 ends can be deep in each reacting hole of reaction utensil 5 and carry out liquid feeding and imbibition, also can be lifted away from reaction utensil 5.
Liquid distribution also can all around be moved with liquid discharge structure 3 and fluid pipeline 4, like this, can realize batch processing to reaction utensil hole matrix, and efficiency is higher.
As shown in Figure 1, according to actual needs, fluid pipeline 4 can be one, also can be many. when fluid pipeline 4 is many, they can be arranged in a row equally spacedly, when liquid feeding or imbibition, each fluid pipeline 4 is corresponding one by one with the reacting hole of a certain row in reaction utensil 5 matrixes or each reaction utensil 5 in certain a line.
Certainly, in some other embodiment of the present invention, when the quantity of fluid pipeline 4 is abundant, many fluid pipelines 4 also can be arranged in matrix form, and each fluid pipeline 4 in this matrix is corresponding one by one with the reacting hole of each reaction utensil 5 in reaction utensil 5 matrixes.
In addition, also comprise: waste collection and harmless treating apparatus 6, for collecting from the waste liquid of reaction utensil 5 sucking-offs, and carry out harmless treatment to waste liquid.In general, the toxicity of waste liquid is all very strong, very large to the harm of human body and physical environment, by this waste collection and 6 pairs of waste liquids of harmless treating apparatus, is undertaken, after harmless treatment, can realizing qualified discharge, safety and environmental protection.
In this waste collection and harmless treating apparatus 6, experimental procedure starts the first step and just automatically adds in advance liquid waste processing liquid.In concrete operating process, can, according to different reactant liquor used, in waste collection, carry out harmless treatment with automatically adding in advance corresponding liquid waste processing liquid in harmless treating apparatus 6.For example, when reactant liquor is diaminobenzidine (DAB), in waste collection and harmless treating apparatus 6, add in advance hydrogen peroxide or 84 thimerosals etc. just can realize harmless treatment to hypertoxic DAB containing the material of active oxygen.
Also comprise: casing 1 is the confined space of 4 degrees Celsius of constant temperature and lucifuge in this casing 1, full-automatic immunochemistry instrument is arranged in this confined space.The environment of 4 degrees Celsius of constant temperature and lucifuge, can guarantee that this full-automatic immunochemistry instrument, under any natural conditions, can work for a long time, and the samples such as reactant liquor and histotomy, cultured cell can keep optimum performance.
4 degrees Celsius of constant temperature, can guarantee variously to prepare stand-by hydraulic fluid in a kind of stable state, extend the expiration date; Under the constant condition of this kind of low temperature, can make speed that immune response carries out slowly, in conjunction with special, reduce local background signal; The environment of lucifuge, is in order to meet when carrying out Immunofluorescence Reactions, the requirement of fluorescent reagent to light.
When liquid distributes the waste liquid passing through in fluid pipeline 4 sucking-off reaction utensils 5 with liquid discharge structure 3, can allow to stay a part of waste liquid, do not inhale too dryly, in order to avoid the samples such as histotomy or cultured cell are sucked away, also can prevent that histotomy or cell sample from dehydrating simultaneously, the effect of impact operation, can solve the residual problem of waste liquid by the method for utilizing washing lotion to wash several times more.
In addition, for fear of samples such as histotomy or cultured cells in the process of imbibition, siphoned away simultaneously, when liquid distributes the waste liquid passing through in fluid pipeline 4 sucking-off reaction utensils 5 with liquid discharge structure 3, should inhale at leisure, the imbibition mouth of pipe of fluid pipeline 4 ends also should be tried one's best carefully.
Also comprise: be arranged on reaction utensil 5 top electrodes 7, specifically the bottom in ，Min hole and loins are mounted with electrode 7 that this electrode 7 adds positive electricity to reaction utensil 5 during for waste liquid at sucking-off reaction utensil 5.Albumen in histotomy sample self is electronegative, by reaction utensil 5 is added after positive electricity, and positive and negative attracting each other, thus guarantee that histotomy sample can not be sucked away when sucking-off waste liquid.
Also comprise: shaking table 8, the top panel of this shaking table 8 is established screens, and the corresponding reaction utensil 5 of each screens or Tissue Culture Dish, culture plate, for shaking reaction utensil, Tissue Culture Dish or culture plate.
Above this shaking table 8, there is screens, Tissue Culture Dish, culture plate or special reaction utensil position can be fixed.
By shaking of shaking table 8, can make liquid fully mix, avoid the samples such as a plurality of histotomies in reaction utensil 5 or cultured cell to be pasted together so that the samples such as histotomy or cultured cell and reactant liquor fully, uniform contact, guarantee to hatch balanced consistent.
Whole immunochemistry operating process is by programmed control, and program can be write.
As shown in Figure 1, this full-automatic immunochemistry instrument also comprises program control instrument 9 and display screen 10, pre-set program, also can provide the possibility of newly establishing program, by program control instrument 9, can carry out full automatic working to this full-automatic immunochemistry instrument, accurately, convenient, and avoided experimenter directly to contact the risk that noxious material brings.By display screen 10, can clearly observe state, the reactant liquor of using of immunochemistry operation and react the required information such as time, so that experimenter grasps experiment process.
In addition, be appreciated that program control instrument 9 and display screen 10 can not have yet in some other embodiment of the present invention.
Compare with pure manual immunochemistry operating process in prior art, this full-automatic immunochemistry instrument can be realized overall process automatic liquid-feeding and sucking-off waste liquid, by changing the mode in Ye Buhuanmin hole, can avoid frequent moving tissue section sample in operating process and it is caused to damage, displacement, and, also can avoid experimenter directly contact noxious material and health is damaged, increase experiment flux, improve conventional efficient, reduce experimental error.
Second embodiment of the invention relates to a kind of using method of full-automatic immunochemistry instrument.Fig. 2 is the schematic flow sheet of the using method of this full-automatic immunochemistry instrument.The using method of this full-automatic immunochemistry instrument is for full-automatic immunochemistry instrument mentioned above
Specifically, as shown in Figure 2, the using method of this full-automatic immunochemistry instrument comprises the following steps:
In step 201, liquid waste processing liquid is joined in waste collection and harmless treating apparatus, reactant liquor is joined in reaction utensil.
After this enter step 202, fully after reaction, by waste liquid sucking-off from reaction utensil.
After this enter step 203, washing lotion is joined in reaction utensil, after fully cleaning, by waste liquid sucking-off from reaction utensil.
In the present embodiment, preferably, step 203 is carried out three times.
By waste liquid from reaction utensil during sucking-off, retain a part of waste liquid, do not inhale too dryly, the object of doing like this, can avoid the sample such as histotomy or cultured cell in sucking-off waste liquid to be siphoned away on the one hand simultaneously, also can prevent from the other hand inhaling too dry after, the samples such as histotomy or cultured cell dehydrate, the effect of impact operation.In the present embodiment, can add the method that washing lotion washes several times more by utilization and solve the residual problem of waste liquid, in the present embodiment, preferably, add washing lotion and wash three times.
Certainly, this is of the present invention preferred embodiment a kind of, in some other embodiment of the present invention, also can only clean once, twice, or clean more number of times, as four times, five times etc.
After this enter step 204, judge whether immunochemistry operation all completes.
If so, process ends; If not, enter step 205, next reactant liquor is added in reaction utensil.
If immunochemistry operation does not all complete yet, next reactant liquor is joined in reaction utensil.
After this again get back to step 202.
Present embodiment is the method embodiment corresponding with the first embodiment, present embodiment can with the enforcement of working in coordination of the first embodiment.The correlation technique details of mentioning in the first embodiment is still effective in the present embodiment, in order to reduce repetition, repeats no more here.Correspondingly, the correlation technique details of mentioning in present embodiment also can be applicable in the first embodiment.
Each method embodiment of the present invention all can be realized in modes such as software, hardware, firmwares.No matter the present invention realizes with software, hardware or firmware mode, instruction code can be stored in the storer of computer-accessible of any type (for example permanent or revisable, volatibility or non-volatile, solid-state or non-solid-state, fixing or removable medium etc.).Equally, storer can be for example programmable logic array (Programmable Array Logic, be called for short " PAL "), random access memory (Random Access Memory, be called for short " RAM "), programmable read only memory (Programmable Read Only Memory, be called for short " PROM "), ROM (read-only memory) (Read-Only Memory, be called for short " ROM "), Electrically Erasable Read Only Memory (Electrically Erasable Programmable ROM, be called for short " EEPROM "), disk, CD, digital versatile disc (Digital Versatile Disc, be called for short " DVD ") etc.
Although pass through with reference to some of the preferred embodiment of the invention, the present invention is illustrated and described, but those of ordinary skill in the art should be understood that and can do various changes to it in the form and details, and without departing from the spirit and scope of the present invention.
Claims (10)
1. a full-automatic immunochemistry instrument, is characterized in that, comprising: at least three reagent bottles (2), liquid distribute and liquid discharge structure (3), at least one fluid pipeline (4) and at least one porous reaction ware (5);
Reagent bottle (2) is for holding reactant liquor, washing lotion and liquid waste processing liquid;
Liquid distributes and liquid discharge structure (3) is connected between reagent bottle (2) and fluid pipeline (4), for the liquid of reagent bottle (2) is injected in the reacting hole of reaction utensil (5) by fluid pipeline (4), or the waste liquid in reaction utensil (5) is passed through to fluid pipeline (4) sucking-off.
2. full-automatic immunochemistry instrument according to claim 1, is characterized in that, each reacting hole of described reaction utensil (5) is that oral area is circular groove shapes, and mouth diameters is 1 centimetre to 10 centimetres;
Between a plurality of reacting holes of reaction utensil (5), be matrix form and arrange, the line space of this matrix equates with the distance between each fluid pipeline (4).
3. full-automatic immunochemistry instrument according to claim 2, is characterized in that, described liquid distributes with liquid discharge structure (3) and fluid pipeline (4) and can move up and down.
4. full-automatic immunochemistry instrument according to claim 3, is characterized in that, described liquid distributes with liquid discharge structure (3) and fluid pipeline (4) and can all around move.
5. full-automatic immunochemistry instrument according to claim 4, it is characterized in that, also comprise: waste collection and harmless treating apparatus (6), in this waste collection and harmless treating apparatus (6), automatically add in advance treating fluid, for collecting the waste liquid from reaction utensil (5) sucking-off, and waste liquid is carried out to harmless treatment.
6. full-automatic immunochemistry instrument according to claim 5, is characterized in that, also comprises: casing (1) is the confined space of 4 degrees Celsius of constant temperature and lucifuge in this casing (1), and described full-automatic immunochemistry instrument is arranged in this confined space.
7. full-automatic immunochemistry instrument according to claim 6, is characterized in that, also comprises: be arranged on reaction utensil (5) top electrode (7), this electrode (7) adds positive electricity to reaction utensil (5) during for waste liquid at sucking-off reaction utensil (5).
8. full-automatic immunochemistry instrument according to claim 7, is characterized in that, also comprises: shaking table (8), and the top panel of this shaking table (8) is established screens, and the corresponding reaction utensil (5) of each screens, for shaking reaction utensil (5).
9. a using method for full-automatic immunochemistry instrument, the full-automatic immunochemistry instrument for as described in claim 1 to 8 any one, is characterized in that, comprises the following steps:
Reactant liquor is joined in reaction utensil, fully after reaction, by waste liquid sucking-off from reaction utensil;
Washing lotion is joined in reaction utensil, after fully cleaning, by waste liquid sucking-off from reaction utensil;
If immunochemistry operation does not all complete yet, next reactant liquor is joined in reaction utensil.
10. the using method of full-automatic immunochemistry instrument according to claim 9, is characterized in that, described washing lotion is joined in reaction utensil, after fully cleaning, the step of waste liquid sucking-off from reaction utensil is carried out three times.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2170520Y (en) * | 1993-06-16 | 1994-06-29 | 陆雪龙 | Automatic operating apparatus for determining radioimmunity |
| CN2444230Y (en) * | 2000-05-16 | 2001-08-22 | 陈智 | Immunohistochemistry apparatus |
| JP2002189033A (en) * | 2000-12-22 | 2002-07-05 | Furuno Electric Co Ltd | Method and system for dispensing, and tip stocker device |
| CN2859530Y (en) * | 2005-12-29 | 2007-01-17 | 王维锋 | Protein mark immunological automatic detection |
| CN101865927A (en) * | 2009-04-17 | 2010-10-20 | 武汉医尔特科技有限公司 | Novel full-automatic blood type analyzer |
| JP2011153940A (en) * | 2010-01-28 | 2011-08-11 | Hitachi High-Technologies Corp | Automatic analyzer |
-
2012
- 2012-06-20 CN CN201210206355.7A patent/CN103513024B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2170520Y (en) * | 1993-06-16 | 1994-06-29 | 陆雪龙 | Automatic operating apparatus for determining radioimmunity |
| CN2444230Y (en) * | 2000-05-16 | 2001-08-22 | 陈智 | Immunohistochemistry apparatus |
| JP2002189033A (en) * | 2000-12-22 | 2002-07-05 | Furuno Electric Co Ltd | Method and system for dispensing, and tip stocker device |
| CN2859530Y (en) * | 2005-12-29 | 2007-01-17 | 王维锋 | Protein mark immunological automatic detection |
| CN101865927A (en) * | 2009-04-17 | 2010-10-20 | 武汉医尔特科技有限公司 | Novel full-automatic blood type analyzer |
| JP2011153940A (en) * | 2010-01-28 | 2011-08-11 | Hitachi High-Technologies Corp | Automatic analyzer |
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