CN211856603U - Integrated immunofluorescence antibody incubation, film washing and drying automation instrument - Google Patents
Integrated immunofluorescence antibody incubation, film washing and drying automation instrument Download PDFInfo
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- CN211856603U CN211856603U CN202020138224.XU CN202020138224U CN211856603U CN 211856603 U CN211856603 U CN 211856603U CN 202020138224 U CN202020138224 U CN 202020138224U CN 211856603 U CN211856603 U CN 211856603U
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Abstract
The utility model relates to a medical detection apparatus technical field particularly relates to an integrated form immunofluorescence antibody is hatched, is washed piece, is dried by the fire piece automation appearance. The utility model relates to an integrated form immunofluorescence antibody is hatched, the developing, dry by the fire automatic appearance of piece, which comprises an outer shell, antibody hatching module, developing module and control cabinet, antibody hatching module include at least one deck constant temperature mechanism, constant temperature mechanism includes at least one thermostatic chamber, is equipped with antibody hatching box in the thermostatic chamber, and developing module includes at least one deck developing mechanism, and developing mechanism includes developing chamber and stock solution room, is equipped with the developing box in the developing chamber, is equipped with the feed liquor pipe on the stock solution room, and the feed liquor pipe passes the lateral wall of developing chamber and stock solution room, and the one end of feed liquor pipe is located the stock solution room, and the other end is located the top of developing box. The utility model can replace scientific research personnel to automatically complete a plurality of operations in batches at the same time, which is helpful to improve the experimental efficiency and save manpower and material resources; and the stability is strong, and the damage of instrument equipment can not be caused.
Description
Technical Field
The utility model relates to a medical detection apparatus technical field particularly relates to an integrated form immunofluorescence antibody is hatched, is washed piece, is dried by the fire piece automation appearance.
Background
Immunofluorescence is the earliest developed one of the marker-based immunology technologies, and is a technology established on the basis of immunology, biochemistry and microscopy. Since the antigen-antibody reaction has high specificity, when an antigen-antibody reaction occurs, one factor can be found out by knowing the other factor. Therefore, the basic principle of immunofluorescence technique is to label the antibody (or antigen) with a fluorescent dye that does not affect the activity of the antigen-antibody, and then to bind with the corresponding antigen (or antibody), to show a specific fluorescence reaction under a fluorescence microscope. The method has wide application in scientific research in various fields and has an extremely important position. Although the basic principle of the immunofluorescence technique is easy to understand, the actual operation steps are complicated, the cost is long, especially in the processes of taking materials, freezing slices, using confining liquid, adding antibodies for incubation, repeatedly washing the slices with buffer solution and drying the slices by drying, people are required to continuously operate on one side, a large amount of unnecessary time is consumed, and the efficiency is relatively low. In daily scientific research, an independent constant temperature box is commonly used for sealing at 37 ℃ or incubating for secondary antibodies, the constant temperature box is simple in structure, but large in size, and is opened, if different subject groups perform experiments, the constant temperature box is frequently opened and closed, mutual interference is easy to perform, effective independent automatic timing cannot be achieved, and experimental results are affected. In the washing stage, the slide glass is put on a staining rack, then put in a staining jar with buffer solution, and rinsed on a shaking table, so that the operation is simple and convenient, but a specially-assigned person is required to replace the washing buffer solution at regular time, the time and the labor are wasted, and batch operation cannot be performed. In the drying stage of the sheet, the method may be different in each laboratory, but the sheet is mainly wiped by a paper towel to be naturally dried, or the sheet is dried by blowing air by an upper fan in midway.
The existing instruments mainly comprise a water bath incubator, a dyeing rack and a dyeing cylinder, and the MERGIITE is an automatic cleaning instrument for indirect immunofluorescence sheets. Wherein the water bath thermostated container is traditional antibody incubation and closed stage constant temperature equipment, and the use is extensive, convenient operation, and accommodation space is big. However, the device has the defects of large volume, single opening and poor sealing performance, and if experiments are carried out on different subject groups, the constant temperature boxes are frequently opened and closed, so that mutual interference is easy to carry out, effective independent automatic timing cannot be realized, and the experimental results are influenced.
The staining rack and the staining jar have the advantages that the slide glass is flexibly placed and taken in and out in the washing stage, the operation is simple, and the fine adjustment can be carried out on the rinsing fold part of the slide glass in real time. However, the special person is needed to replace the cleaning buffer solution at regular time, which wastes time and labor and can not be operated in batch. Compared with the traditional manual cleaning mode, the German research and development product is independently and synchronously cleaned by the 050 channel, has better cleaning quality and is not easy to damage the tissue obtained from the material. The film washing machine has the advantages of being not expensive in selling price, not easy to popularize, single in function, only capable of taking the film for washing, incapable of continuously performing multi-step operation and not greatly improved in the aspect of saving time.
Therefore, an integrated immunofluorescence antibody incubation, washing and drying automation instrument is needed at present to replace scientific research personnel to automatically complete a plurality of operations in batches at the same time.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem, the utility model adopts the following technical scheme:
the utility model provides an integrated form immunofluorescence antibody is hatched, is washed piece, dries by fire piece automatic appearance, includes the shell, antibody incubation module, and washing piece module and control cabinet, antibody incubation module include at least one deck constant temperature mechanism, constant temperature mechanism include at least one thermostatic chamber, the thermostatic chamber in be equipped with antibody incubation box, the washing piece module include at least one deck washing piece mechanism, washing piece mechanism include washing piece room and stock solution room, the washing piece indoor be equipped with the washing piece box, the stock solution room on be equipped with the feed liquor pipe, the feed liquor pipe pass the lateral wall of washing piece room and stock solution room, the one end of feed liquor pipe is located the stock solution room on, the other end is located the top of washing piece box.
The utility model discloses a set up multilayer constant temperature mechanism and multilayer developing mechanism, can hatch simultaneously in batches, developing, dry by the fire the piece operation like this, help improving experimental efficiency, and antibody hatching box can continuously carry out constant temperature operation, need not often to open and shut and help improving the precision of experiment, has set up the stock solution room simultaneously, is convenient for directly carry out the developing to the injection buffer solution in the developing box, has improved the efficiency of developing.
Preferably, the bottom of the thermostatic chamber is provided with a heating system, the heating system comprises a water bath heating pipe, the water bath heating pipe is electrically connected with the console, and a steel wire mesh is arranged above the water bath heating pipe.
The utility model discloses a water bath heating pipe heats, can guarantee the temperature stability in the constant temperature chamber, has guaranteed the utility model discloses a stability of use, simultaneously the utility model discloses a wire netting structure can play the supporting role to antibody hatching box, strengthens its stability.
Preferably, the developing box is provided with a liquid outlet pipe, one end of the liquid outlet pipe is fixedly connected with the bottom of the developing box, and the other end of the liquid outlet pipe penetrates through the side wall of the developing box and is positioned on the outer side of the shell.
Through this structure can be convenient for the change of liquid in the developing cartridge, improved the utility model discloses an availability factor has just prevented to take out the developing cartridge and has changed and cause the buffer solution to flow to the developing cartridge outside and cause the pollution to the developing chamber indoor.
Preferably, the bottom of the film developing chamber is provided with a shaking table.
The utility model discloses a structure of shaking table waves the developing solution box through the shaking table structure, has strengthened the utility model discloses an efficiency and the effect of developing solution, simultaneously, can wash the slide glass in the developing solution box automatically, have reduced experimenter's operation, have practiced thrift the manpower.
Preferably, the top of the film developing chamber is fixedly connected with a heating device, the heating device comprises a fan shell, a heating wire and a fan, the heating wire and the fan are arranged in the fan shell, an air outlet is formed in the fan shell, and the heating wire and the fan and the air outlet are positioned on the same straight line.
The utility model discloses in the use, can be through the buffer liquid of drain pipe in with the film developing box, then blow in hot-blast film developing box through heater strip and fan in, air-dry the slide glass, improved the utility model discloses an availability factor.
Preferably, fixing grooves are formed in two sides of the antibody incubation box, and fixing blocks matched with the fixing grooves are arranged on the inner wall of the thermostatic chamber.
The utility model discloses set up the fixed slot in the both sides of antibody hatching box, when needing to take out antibody hatching box, can slide antibody hatching box for it breaks away from the inner wall of thermostatic chamber, can go into the fixed slot with the fixed block card when fixed in, makes it accomplish firm fixed.
Preferably, the liquid inlet pipe and the liquid outlet pipe are both provided with a micro water pump, and the micro water pumps are electrically connected with the console.
The utility model adopts the micro water pump to control the liquid in the liquid inlet pipe and the liquid outlet pipe to pass in and out, which can improve the operation efficiency of the utility model, thereby being convenient for the use of the utility model, and simultaneously, the utility model is operated by the control console, simple and practical,
preferably, the shaking table is provided with a shaking rod, and the shaking rod is connected with the film developing box.
The utility model discloses a for small-size shaking table, be equipped with single rocking pole on it and be connected with the film developing box, this rocking pole can drive the film developing box and rock, has the efficient advantage of rocking.
Preferably, the bottom of the film washing box is provided with a rocker fixing groove, one end of the rocker fixing groove is provided with an opening, the top of the rocker rod is provided with a rocker fixing block, and the rocker is connected in the rocker fixing groove in a high-bandwidth sliding manner.
Because the utility model discloses every film washing box's space is less, the in-process that rocks at the film washing box collides with the lateral wall of film washing box easily, because the slide glass is cracked more easily, consequently this scheme that this application adopted can strengthen the fixed effect of film washing box and rocking rod, prevents that the film washing box from taking place to remove and leads to the slide glass to receive vibrations and take place cracked.
The beneficial effects of the utility model reside in that: 1. the device can replace scientific research personnel to automatically complete a plurality of operations in a plurality of batches at the same time, thereby improving the experimental efficiency and being beneficial to saving manpower and material resources; 2. a plurality of subject groups can be subjected to experiments simultaneously; 3. the device has the advantages of strong stability and no damage to instruments and equipment.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural view of a thermostatic chamber;
FIG. 3 is a schematic structural view of a film processing mechanism;
FIG. 4 is a schematic structural view of a heating device;
FIG. 5 is a schematic view of the bottom of the cassette.
Detailed Description
The present invention will be further explained with reference to the following embodiments.
Examples
The utility model provides an integrated form immunofluorescence antibody is hatched, the developing, dry by the fire piece automatic appearance, including shell 1, antibody hatching module 2, developing module 3 and control cabinet 4, antibody hatching module 2 includes four layers of constant temperature mechanism 21, each layer of constant temperature mechanism 21 is including two thermostats 211 that parallel, be equipped with antibody hatching box 5 in the thermostats 211, developing module 3 includes four layers of developing mechanism 31, every layer of developing mechanism 31 includes developing room 311 and stock solution room 312, is equipped with developing box 6 in developing room 311, is equipped with feed liquor pipe 7 on the stock solution room 312, stock solution room 312 swing joint have sealed lid 3121, the utility model discloses can be through opening sealed lid and directly supply the buffer solution to every layer of stock solution room, feed liquor pipe 7 passes the lateral wall of developing room 311 and stock solution room 312, the one end of feed liquor pipe 7 is located stock solution room 312, the other end is located developing box 6's top. The bottom of the thermostatic chamber 211 is provided with a heating system 2111, the heating system 2111 comprises a water bath heating pipe 21111, the water bath heating pipe 21111 is electrically connected with the console 4, and a steel wire mesh 21112 is arranged above the water bath heating pipe 21111. Be equipped with drain pipe 8 on the film processing box 6, the one end fixed connection of drain pipe 8 is in the bottom of film processing box 6, and the other end of drain pipe 8 passes the outside that film processing room 311 lateral wall is located shell 1 to be connected with the waste liquid jar. The bottom of the film washing chamber 311 is provided with a shaker 3112. The top of the film washing compartment 311 is fixedly connected with a heating device 3111, the heating device 3111 comprises a fan housing 31111, a heating wire 31112 and a fan 31113, the heating wire 31112 and the fan 31113 are arranged in the fan housing 31111, an air outlet is arranged on the fan housing 31112, and the heating wire 31112, the fan 31113 and the air outlet are arranged on the same straight line. Fixing grooves 51 are formed in two sides of the antibody incubation box 5, two ends of each fixing groove 51 are open, fixing blocks 2112 matched with the fixing grooves 51 are arranged on the inner wall of the constant-temperature chamber 211, and the fixing blocks 2112 are connected in the fixing grooves 51 in a sliding mode. The liquid inlet pipe 7 and the liquid outlet pipe 8 are both provided with a micro water pump 9, and the micro water pump 9 is electrically connected with the control console 4. The shaking table 3112 is provided with a shaking rod 31121, the bottom of the film washing box 6 is provided with a shaking bar fixing groove 61, one end of the shaking bar fixing groove 61 is provided with an opening, the top of the shaking bar 31121 is provided with a shaking bar fixing block 311211, and the shaking bar fixing block 311211 is slidably connected in the shaking bar fixing groove 61.
In the use process of the utility model, each subject group can use one layer of constant temperature mechanism 21 and one layer of developing mechanism 31, each layer of constant temperature mechanism 21 and developing mechanism 31 operate independently, when in use, the antibody incubation box 5 can be inserted into the constant temperature chamber 211, the fixing block 2112 is clamped into the fixing groove 51 to realize the fixation of the antibody incubation box 5, then the switch of the water bath heating pipe 21111 is controlled through the control console 4 to control the temperature in the constant temperature chamber 211, meanwhile, the glass slide in the antibody incubation box 5 can be cleaned in the developing chamber 311, the micro water pump 9 is controlled through the control console 4 to inject the buffer solution into the developing box 6, the shaking table 3112 is opened to clean, then the micro water pump 9 can be controlled by the control console 4 to discharge the buffer solution, then the developing box 6 can be taken out, then the glass slide is taken out, then the rocker fixing block 311211 is clamped into the rocker fixing groove 61 at the bottom of the developing box 6, accomplish the installation, realize the utility model discloses a use. The utility model discloses have and can replace scientific research personnel to accomplish a great deal of operation simultaneously many batches automatically, improving experimental efficiency, help practicing thrift the manpower, the advantage of material resources.
Claims (9)
1. The utility model provides an integrated form immunofluorescence antibody is hatched, is washed piece, is dried by the fire piece automation equipment, includes the shell, and antibody hatching module, washing piece module and control cabinet, its characterized in that: the antibody incubation module include at least one deck constant temperature mechanism, constant temperature mechanism include at least one constant temperature room, the constant temperature room in be equipped with antibody incubation box, the developing module include at least one deck developing mechanism, developing mechanism include developing room and stock solution room, the developing room in be equipped with the developing box, the stock solution room on be equipped with the feed liquor pipe, the feed liquor pipe pass the lateral wall of developing room and stock solution room, the one end of feed liquor pipe is located the stock solution room on, the other end is located the top of developing box.
2. The integrated immunofluorescence antibody incubation, washing and drying automation instrument according to claim 1, wherein: the bottom of thermostatic chamber be equipped with heating system, heating system include the water bath heating pipe, the water bath heating pipe with the control cabinet electricity be connected, water bath heating pipe top be equipped with the wire net.
3. The integrated immunofluorescence antibody incubation, washing and drying automation instrument according to claim 2, wherein: the developing box on be equipped with the drain pipe, the one end fixed connection of drain pipe in the bottom of developing box, the other end of drain pipe pass developing box lateral wall be located the outside of shell.
4. The integrated immunofluorescence antibody incubation, washing, drying automation instrument according to claim 3, wherein: and a shaking table is arranged at the bottom of the film developing chamber.
5. The integrated immunofluorescence antibody incubation, washing, drying automation instrument according to claim 4, wherein: the top fixedly connected with heating device of film developing room, heating device include fan housing, heater strip and fan be in fan housing in, fan housing on be equipped with the air outlet, the heater strip with fan and air outlet be located same straight line.
6. The integrated immunofluorescence antibody incubation, washing, drying automation instrument according to claim 5, wherein: the two sides of the antibody incubation box are provided with fixing grooves, and the inner wall of the thermostatic chamber is provided with fixing blocks matched with the fixing grooves.
7. The integrated immunofluorescence antibody incubation, washing, drying automation instrument according to claim 6, wherein: the liquid inlet pipe and the liquid outlet pipe are both provided with a micro water pump, and the micro water pumps are electrically connected with the control console.
8. The integrated immunofluorescence antibody incubation, washing, drying automation instrument according to claim 4, wherein: the shaking table is provided with a shaking rod, and the shaking rod is connected with the film developing box.
9. The integrated immunofluorescence antibody incubation, washing, drying automation instrument according to claim 8, wherein: the bottom of the film washing box is provided with a rocker fixing groove, one end of the rocker fixing groove is provided with an opening, the top of the rocker rod is provided with a rocker fixing block, and the rocker is connected in the rocker fixing groove in a high-bandwidth sliding manner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020138224.XU CN211856603U (en) | 2020-01-21 | 2020-01-21 | Integrated immunofluorescence antibody incubation, film washing and drying automation instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020138224.XU CN211856603U (en) | 2020-01-21 | 2020-01-21 | Integrated immunofluorescence antibody incubation, film washing and drying automation instrument |
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| CN211856603U true CN211856603U (en) | 2020-11-03 |
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| CN202020138224.XU Active CN211856603U (en) | 2020-01-21 | 2020-01-21 | Integrated immunofluorescence antibody incubation, film washing and drying automation instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112747991A (en) * | 2020-12-23 | 2021-05-04 | 山东骏腾医疗科技有限公司 | Full-automatic drop dyeing device for tissue slices |
-
2020
- 2020-01-21 CN CN202020138224.XU patent/CN211856603U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112747991A (en) * | 2020-12-23 | 2021-05-04 | 山东骏腾医疗科技有限公司 | Full-automatic drop dyeing device for tissue slices |
| CN112747991B (en) * | 2020-12-23 | 2023-04-07 | 山东骏腾医疗科技有限公司 | Full-automatic drop dyeing device for tissue slices |
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