CN209311186U - A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing - Google Patents

A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing Download PDF

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Publication number
CN209311186U
CN209311186U CN201822019251.0U CN201822019251U CN209311186U CN 209311186 U CN209311186 U CN 209311186U CN 201822019251 U CN201822019251 U CN 201822019251U CN 209311186 U CN209311186 U CN 209311186U
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China
Prior art keywords
liquid
objective table
dyeing
storage bottle
shunting disk
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Expired - Fee Related
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CN201822019251.0U
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Chinese (zh)
Inventor
杨柳
马洁
楼敏铭
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Tianjin Kangting Biological Engineering Group Co Ltd
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Tianjin Kangting Biological Engineering Group Co Ltd
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Abstract

The utility model relates to it is a kind of suitable for Various Tissues batch dyeing HE dyeing apparatus, including stainless steel multilayer support frame, be protected from light liquid storage bottle, washing lotion storage bottle, rounded porous shunting disk, dropping liquid conduit, support under casing, angle rotatable objective table.The utility model goes out rounded porous shunting disk is arranged in liquid communication conduit, is similar to bathing head shower head formula structure, adds a plurality of drainage tube, drainage tube is equipped with switch, can be according to experiment needs, the quantity of appropriate regulating switch, batch dyeing is carried out simultaneously, reduces the operating time;Angle rotatable objective table is installed in pulley, and the adjusting of position and angle can be carried out by handle, glass slide card slot and waste liquid drainage channel is arranged on objective table, while can be good at fixed glass slide, solves the problems, such as the mutual pollution between glass slide.

Description

A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing
Technical field
The utility model belongs to the experimental apparatus for research equipment technical field such as histopathology and molecular biology, mainly It is related to HE dye experimental provision, is especially adapted for use in Various Tissues mass and carries out HE dyeing.
Background technique
With commonly used, the processing and higher and higher, the group of dyeing quality requirement to tissue pathological slice of pathological diagnosis It knits dicing method to gradually extend to teaching and in scientific experiment, have evolved into a kind of universal experimental method, HE, which is dyed, is then Most common colouring method during making slice, HE staining technique are one kind based on form, connected applications chemistry The experimental technique that technology dyes tissue, various cells, is the fields such as histology, embryology, pathology and biology Teaching and most basic, most popular technical method in scientific research, for paraffin section, the frozen section being attached on glass slide And the specimen such as cell are dyed, for studying histiocytic physiology, pathology and chemical structure, in pathologic finding and teaching section Very crucial effect is played in grinding.
HE decoration method, i.e. hematoxylin eosin staining method, the basic principle is that: hematoxylin H dye liquor is alkalinity, molten in alkalinity Blue in liquid, there are many type for the amino acid that the protein in tissue constitutes protein, they have different isoelectric points, cell The chromatin of interior acidic materials such as nucleus, intracytoplasmic nucleic acid, gland cell and rough surfaced endoplasmic reticulum (RER) in nerve cell and thoroughly The basophillas substance such as bright cartilage matrix, with positively charged hematoxylin basic dye with ionic bond in conjunction with and be dyed to hyacinthine, Indigo plant is shown after being broken up with acidic ethanol, when processing is suitable for, can make nucleus clearly navy blue, other ingredients decolorations such as endochylema. Eosin Y is a kind of chemically synthesized acid dyes, is dissociated into negatively charged anion in water, the amino positive electricity with protein The cation of lotus, which combines, dyes endochylema, the hemoglobin in other oroteins such as red blood cell in cytoplasm, muscle, connective group Knit, the particle of eosinophils and the acidophilus type substance such as collagenous fibres and muscle fibre show the different pink of the depth, with indigo plant The nucleus of color forms sharp contrast, makes the gross morphology design feature of various tissues or cell component and lesion can be clearly Show.
In HE staining technique, paraffin section experiment include material acquisition and segmentation, fixation, dehydration, transparent, waxdip, It embeds, repairs block, slice, bonding die, dewaxing, dyeing, is dehydrated transparent and mounting, material is cut into very thin piece, can reach 2~8 Microns, and continuous piece can be made keep the integrality of tissue, thus become in the tabletting technology of optical microscopy compared with One of common method, dyeing time in dyeing course, dyeing temperature, the pH value of dyeing liquor and dyeing liquor effective concentration can Dyeing effect is influenced, there has been no HE to dye dedicated unit at present, in existing Cytobiology and molecular biology scientific experiment, usually Draw dyeing liquor using pasteur pipet or rubber head dropper, carry out sample dyeing one by one, cannot apparatus for placing everywhere, it is existing not Good dyeing apparatus can increase experimenter's operation difficulty, extend the operating time, when carrying out batch sample dyeing, between dyeing time Every longer, it is easy that sample is made to cross dye, leads to undesirable dyeing effect.
In conclusion need gradually to improve HE dyeing apparatus in clinical application and scientific experiment, improve sample film-making and Dyeing quality improves experiment condition, reduces time cost, ensures experimenter's personal safety, improves HE staining technique, examines for clinic Disconnected and scientific experiment interpretation of result research provides important evidence, to overcome deficiency existing for existing experimental provision, the utility model A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing is provided.
Utility model content
It is a kind of suitable for Various Tissues batch the purpose of the utility model is to overcome providing in place of the deficiencies in the prior art The HE dyeing apparatus of dyeing is able to carry out the dyeing of mass tissue samples, changes the existing apparatus manually status of liquid feeding one by one, existing Scientific experiment in use rubber head dropper or pasteur pipet, the uneven liquid volume added of liquid feeding is big, and device can not be placed arbitrarily, It can solve the inhomogenous problem of liquid feeding using the utility model, while liquid-adding device can be placed independently, be convenient for experimental implementation.
The utility model technical scheme applied to solve the technical problem is:
A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing, it is characterised in that: supported including stainless steel multilayer Frame, be protected from light liquid storage bottle, washing lotion storage bottle, rounded porous shunting disk, dropping liquid conduit, support under casing, angle rotatable objective table, its Specific structure is as follows:
The stainless steel multilayer support frame is three ply board setting up and down, is embedded in multiple keep away on the top of support frame top plate Light liquid storage bottle and a washing lotion storage bottle, are protected from light liquid storage bottle and the equal lower part of washing lotion storage bottle is formed with outlet tube, pacify on outlet tube Solenoid valve is filled, multiple rounded porous shunting disks are installed in support frame mesosphere board, rounded porous shunting disk top is formed with inlet, Rounded porous shunting disk lower part is evenly distributed with multiple liquid droping ports, and inlet, rounded porous shunting disk is arranged in rounded porous shunting disk top Inlet is respectively communicated with by drainage catheter connects the outlet tube for being protected from light liquid storage bottle and washing lotion storage bottle, under rounded porous shunting disk Multiple liquid droping ports in portion are separately connected a dropping liquid conduit, and plastic conical head is arranged in dropping liquid end of conduit, in support frame lower plywood Interior setting supports under casing, supports sliding setting angle rotatable objective table under casing, the setting of angle rotatable objective table upper surface Waste liquid collecting box is arranged in glass slide card slot and objective table waste liquid drainage channel, support under casing inside.
Moreover, the support surface of bottom case surrounding is formed with slideway, pulley is installed in quadrangle under angle rotatable objective table, Pulley slides in slideway, and then the position of mobile angle rotatable objective table, corresponding under different rounded porous shunting disks Portion, support under casing middle part are arranged liquid outlet, waste liquid collecting box are arranged in support under casing cabinet, waste liquid collecting box corresponds to liquid outlet.
Moreover, angle rotatable objective table upper surface interval is formed with glass slide card slot, be embedded in glass slide card slot to Stained slide is formed with waste liquid drainage trough in the angle rotatable objective table upper surface of glass slide card slot front.The utility model Be a little with good effect:
A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing of the utility model, goes out to be arranged in liquid communication conduit Rounded porous shunting disk is similar to bathing head shower head formula structure, adds a plurality of drainage tube, and drainage tube is equipped with switch, Ke Yigen Needs, the quantity of appropriate regulating switch are factually tested, while carrying out batch dyeing, reduces the operating time;Angle rotatable objective table It is installed in pulley, the adjusting of position and angle can be carried out by handle, glass slide card slot is set on objective table and waste liquid draws Circulation road solves the problems, such as the mutual pollution between glass slide while can be good at fixed glass slide.
The utility model uses rounded porous shunting disk, can be according to experiment demand, while dyeing to multiple prints, Batch carries out, and operation efficiently, greatlys save experimental period;2, rounded porous shunts pan bottom and designs multiple plastic conical head dropping liquids Conduit, each dropper set up switch separately again, can select the number turned on the switch according to experiment needs, meet most experiments demand;
The utility model increases pulley and round slide using the bearing-type design in physics, can be realized objective table cross To the adjusting of position and the adjusting of angle, when cleaning, cleaning solution residual is reduced, cleaning solution is avoided vertically to be added dropwise in tissue surface, Reduce cleaning solution to the impact force of tissue, avoids washing lotion from causing to impact to sample, reduce the flake of sample;Reagent is out of liquid storage bottle It is added directly on glass slide by conduit, without other liquid shifting equipments, effectively avoids the secondary pollution of reagent;It is protected from light Liquid storage bottle bottle body uses the material with shading anti-corrosion function, meets high shading, puts burn into volatilization prevention and hygienic needs, can grow Phase stores dyestuff;Increase waste liquid collecting box in package unit end, by the unified recycling of all experimental waste liquids, convenient for keeping environment clean Cleanliness.
Due to being related to chemical reagent during whole operation, to schneiderian membrane and respiratory tract there are certain harm, make After being dyed with the method, operator can be avoided as far as possible and chemical reagent by controlling the program setting and operation that be tested Contact, reduce harm to body;Experimental record is automatically saved, program is set on operation panel, can be automatically recorded whole A experimentation according to coloration result, is concluded dyeing condition, is arranged convenient for filing after dyeing, confirms best experiment item Part;Each control function is respectively provided with sound alarm function, the operation to operator, and system can be carried out judgement, and system is automatically not The operation of mistake is executed, so as to operator's correction;Control panel closed type touches panel, operates reliable, dust-proof, waterproof, It can be controlled at any time in experimental implementation, be shown in state of a control all on liquid crystal display, be convenient for Germicidal efficacy.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Fig. 2 is the structural schematic diagram for supporting under casing and angle rotatable objective table.
Fig. 3 is angle rotatable objective table surface structure schematic diagram.
Fig. 4 is the structural schematic diagram of rounded porous shunting disk.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawing and by specific embodiment, and following embodiment is only retouched The property stated, it is not restrictive, protection scope of this utility model cannot be limited by this.
A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing, including stainless steel multilayer support frame 3, it is protected from light liquid storage Bottle 1, washing lotion storage bottle 2, drainage catheter 4-1, rounded porous shunting disk 4, dropping liquid conduit 5, support under casing 7, angle rotatable carry Object platform 6, its specific structure are as follows:
The stainless steel multilayer support frame is three ply board setting up and down, is embedded in multiple keep away on the top of support frame top plate Light liquid storage bottle and a washing lotion storage bottle, are protected from light liquid storage bottle and the equal lower part of washing lotion storage bottle is formed with outlet tube, pacify on outlet tube Solenoid valve is filled, multiple rounded porous shunting disks are installed in support frame mesosphere board, rounded porous shunting disk top is formed with inlet, Rounded porous shunting disk lower part is evenly distributed with multiple liquid droping ports, and inlet, rounded porous shunting disk is arranged in rounded porous shunting disk top Inlet is respectively communicated with by drainage catheter connects the outlet tube for being protected from light liquid storage bottle and washing lotion storage bottle, under rounded porous shunting disk Multiple liquid droping ports in portion are separately connected a dropping liquid conduit, and plastic conical head is arranged in dropping liquid end of conduit, in support frame lower plywood Interior setting supports under casing, supports sliding setting angle rotatable objective table under casing, the setting of angle rotatable objective table upper surface Waste liquid collecting box is arranged in glass slide card slot 12 and objective table waste liquid drainage channel 11, support under casing inside.
The support surface of bottom case surrounding is formed with slideway 10, and pulley 8 is installed in quadrangle under angle rotatable objective table, sliding Wheel slides in slideway, and then the position of mobile angle rotatable objective table, corresponding in different rounded porous shunting disk lower parts. It supports and liquid outlet 9 is set in the middle part of under casing, for collecting the dyeing liquor dripped on objective table and washing lotion, setting in support under casing cabinet Waste liquid collecting box 13, waste liquid collecting box corresponds to liquid outlet, for dyeing liquor and washing lotion to be uniformly processed.
Angle rotatable objective table upper surface interval is formed with glass slide card slot, and load to be dyed is embedded in glass slide card slot Slide is formed with waste liquid drainage trough in the angle rotatable objective table upper surface of glass slide card slot front, for draining dyeing and washing Liquid, stream dyeing and washing lotion are low to support surface of bottom case, are finally collected into waste liquid collecting box.
It is protected from light liquid storage bottle in the application using anti-corrosion material is protected from light, light-exposed easy decomposed substance and corrosive goods can be held For HE dyeing liquor volatilization prevention and anti-decomposition, and liquid storage bottle sealing cover is arranged in matter, is equipped with liquid storage bottle liquid in liquid storage bottle bottom and leads Liquid storage bottle connect with rounded porous shunting disk using drainage catheter, utilizes drainage catheter switch control liquid communication by head piece. Plastic conical head dropping liquid conduit is sequentially distributed in rounded porous shunting disk, is convenient for dropping liquid.It will be washed when not in use in equipment Liquid storage bottle is covered with washing lotion storage bottle sealing cover, avoids dirt contamination bottle for handling liquid toilet or cosmetic substance.In this dyeing apparatus, stainless steel multilayer is set Support frame, by liquid storage bottle as first layer, drainage catheter is extended to the second layer by aperture in place, and rounded porous shunts Disk is located at the second layer, and angle rotatable objective table and support under casing are located at third layer.By exports coding display, computer monitor Print out equipment is connect with host computer, and last experimental result and experimental arrangement are exported by print out equipment, and output is compiled Time control switch is set in code display, also can recorde experiment number, experiment sum and experimental period.
The application can also have CPU, connect solenoid valve, and the time controls sensor, exports coding display, computer monitor And host computer, control the time that each solenoid valve is opened, come adjust dyeing and washing lotion time and successive program.Before experiment, Time control program software, print out equipment and host computer in opening exports coding display, computer monitor.It will Soviet Union The device for storing liquid that lignin dyeing liquor is dyed with two kinds of eosin stains liquid is identical, and ammonium hydroxide is identical with the device for storing liquid of acidic ethanol, makes Haematoxylin dyeing liquid, eosin stains liquid, ammonium hydroxide and acidic ethanol are first respectively charged into and are protected from light in liquid storage bottle by the used time, are stored up in washing lotion The suitable washing lotion of injection in bottle is deposited, liquid storage bottle is in turn secured on stainless steel multilayer support frame, connects liquid storage with drainage catheter Bottle and rounded porous shunting disk, according to the number of glass slide in experiment, successively according to print number demand by dropping liquid catheter switch It opens, is being placed in it just on angle rotatable objective table, objective table is currently at below haematoxylin dyeing drop pipe, starts to contaminate The glass slide print prepared is placed within glass slide card slot when color, the time control clicked on exports coding display is opened It closes, controlling sensor by the time and open drainage catheter and switch makes dyeing liquor drip to glass slide along plastic conical head dropping liquid conduit On, timing is carried out, when dyeing stops, closing drainage catheter switch, manipulation objective table controls handle, makes objective table pulley along circle Shape slideway laterally moves under washing lotion storage bottle dropper, and rotatable stage controls handle, makes loading using threaded nut formula structure Platform is tilted a certain angle, and is opened drainage catheter switch, is flowed to washing lotion on glass slide, dyeing liquor is cleaned, mixed liquor is along load Object platform waste liquid drainage channel enters in waste liquid collecting box, then objective table is rotated back to horizontal position, then in the same way according to It is secondary to cross ammonium hydroxide, acidic ethanol and eosin stains liquid.
After experiment, support under casing sliding door is opened, the waste liquid in waste liquid collecting box is taken out, is rationally handled.Time The time conditions entirely tested can be shown in control program software, are printed experimental result by print out equipment, carried out Experimental summary, convenient for searching out optimal experiment condition.
Above-described embodiment is the explanation to the utility model, is not the restriction to the utility model, and any pair of sheet is practical new Scheme after type simple transformation belongs to the protection scope of the utility model.

Claims (3)

1. it is a kind of suitable for Various Tissues batch dyeing HE dyeing apparatus, it is characterised in that: including stainless steel multilayer support frame, It is protected from light liquid storage bottle, washing lotion storage bottle, rounded porous shunting disk, dropping liquid conduit, support under casing, angle rotatable objective table, its tool Body structure is as follows:
The stainless steel multilayer support frame is three ply board setting up and down, is embedded on the top of support frame top plate and multiple is protected from light storage Liquid bottle and a washing lotion storage bottle, are protected from light liquid storage bottle and the equal lower part of washing lotion storage bottle is formed with outlet tube, install electricity on outlet tube Magnet valve installs multiple rounded porous shunting disks in support frame mesosphere board, and rounded porous shunting disk top is formed with inlet, round Porous shunting disk lower part is evenly distributed with multiple liquid droping ports, and inlet, rounded porous shunting disk feed liquor is arranged in rounded porous shunting disk top Mouth is respectively communicated with the outlet tube that connection is protected from light liquid storage bottle and washing lotion storage bottle by drainage catheter, rounded porous shunting disk lower part Multiple liquid droping ports are separately connected a dropping liquid conduit, and dropping liquid end of conduit is arranged plastic conical head, sets in support frame lower plywood Support under casing is set, sliding setting angle rotatable objective table under casing is supported, the setting of angle rotatable objective table upper surface carries glass Waste liquid collecting box is arranged in piece card slot and objective table waste liquid drainage channel, support under casing inside.
2. the HE dyeing apparatus according to claim 1 suitable for Various Tissues batch dyeing, it is characterised in that: the branch Support surface of bottom case surrounding is formed with slideway, and pulley is installed in quadrangle under angle rotatable objective table, and pulley slides in slideway, into And the position of angle rotatable objective table is moved, and it is corresponding in different rounded porous shunting disk lower parts, support setting in the middle part of under casing Liquid outlet, support under casing cabinet is interior to be arranged waste liquid collecting box, and waste liquid collecting box corresponds to liquid outlet.
3. it is according to claim 1 suitable for Various Tissues batch dyeing HE dyeing apparatus, it is characterised in that: it is described can Rotation angle objective table upper surface interval is formed with glass slide card slot, glass slide to be dyed is embedded in glass slide card slot, in glass slide The angle rotatable objective table upper surface of card slot front is formed with waste liquid drainage trough.
CN201822019251.0U 2018-12-04 2018-12-04 A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing Expired - Fee Related CN209311186U (en)

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CN201822019251.0U CN209311186U (en) 2018-12-04 2018-12-04 A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109283033A (en) * 2018-12-04 2019-01-29 天津市康婷生物工程集团有限公司 A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing
CN111323285A (en) * 2020-03-11 2020-06-23 桂林医学院 Automatic change dyeing decoloration device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109283033A (en) * 2018-12-04 2019-01-29 天津市康婷生物工程集团有限公司 A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing
CN111323285A (en) * 2020-03-11 2020-06-23 桂林医学院 Automatic change dyeing decoloration device
CN111323285B (en) * 2020-03-11 2022-12-06 桂林医学院 Automatic change dyeing decoloration device

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