CN111323285A - Automatic change dyeing decoloration device - Google Patents

Automatic change dyeing decoloration device Download PDF

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Publication number
CN111323285A
CN111323285A CN202010165865.9A CN202010165865A CN111323285A CN 111323285 A CN111323285 A CN 111323285A CN 202010165865 A CN202010165865 A CN 202010165865A CN 111323285 A CN111323285 A CN 111323285A
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box
dyeing
ring
liquid
adapter
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CN111323285B (en
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尹铁球
彭鹏
任碧琼
杨雪娇
吴超利
杨欢
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Guilin Medical University
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Guilin Medical University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Fiber Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

An automatic dyeing and decoloring device comprises a main body, a fixed liquid box, a silver dyeing liquid box, a developing liquid box, a washing liquid box, a partition plate, a switching ring, a dyeing bin, a dyeing box, a connecting shaft, an air dispersing box, a liquid discharging box, a first handle, a through flow cover, an upright post, a supporting leg, an upper cover, a liquid inlet, a supporting plate, an air vent, a groove, a convex shoulder, a shaking table, a switching sleeve, a supporting piece, a control valve, a motor, a connecting rod, an upper clamping ring and a lower clamping ring; wherein the upper cover is provided with an upper hole and a guide plate; the adapter sleeve comprises a liquid discharge ring, a spacer ring, a recess and a second handle; the main part is internally provided with a supporting plate, four clapboards are arranged above the supporting plate, and four spaces separated by the four clapboards are respectively used for accommodating the fixed liquid box, the silver staining liquid box, the developing liquid box and the washing liquid box.

Description

Automatic change dyeing decoloration device
Technical Field
The invention relates to the field of medical equipment, in particular to an automatic dyeing and decoloring device.
Background
The polyacrylamide gel electrophoresis can separate protein into a plurality of zones according to different mobilities generated by the difference of charges of different biological macromolecules such as protein molecules and the difference of molecular sizes, and if a separated and purified sample only contains the same protein, only one zone is separated after the protein sample is electrophoresed. After electrophoresis, the separated zones need to be stained with a dye for observation, and commonly used dyes include silver nitrate, Coomassie Brilliant blue R250 or G250, and other areas of the gel where no sample is present are also stained during staining, and are easily eluted by an organic solvent, so that the gel needs to be decolorized with a decolorizing solution.
The general dyeing and decoloring process comprises the following steps: placing the gel after electrophoresis in a box, adding a reagent for fixing for a certain time, and pouring out the reagent in the box; washing for a certain time, and pouring out the reagent in the box; dyeing for a certain time, and pouring out the reagent in the box; then repeatedly eluting several times, pouring out the reagent in the box, taking out the gel and storing. When dyeing and decoloring, the box is always placed on a shaking table or an oscillator, on one hand, reagents are uniformly mixed, and on the other hand, the shaking can ensure that the dyeing and decoloring effects are better.
However, in practical use, the following problems exist:
1. in the dyeing and decoloring device in the prior art, the dyeing liquid or the decoloring liquid needs to be continuously replaced for a long time in the dyeing and decoloring processes, the liquid is easily scattered outside the recovery bottle to cause pollution, and the gel is easily cracked due to repeated moving in the liquid replacing process. And the gel, the dyeing solution and the decoloring solution contain a large amount of toxic and harmful substances, and the harmful substances or harmful gases (such as formaldehyde commonly used in the developing solution) are contacted to volatilize and cause harm to human bodies, thereby influencing the body health of operators.
The dyeing and decoloring device in the prior art has many technical difficulties which are not overcome, and the cost is very high due to the fact that a large number of remote control type electromagnetic valves such as a plurality of liquid inlet remote control valves and a plurality of liquid outlet remote control valves are used.
The dyeing and decoloring device of the prior art requires a lot of pipes due to frequent and large flow path circulation, and only considers the functionality such as designing a pipe structure with complicated design inside the device for circulating various dyeing and decoloring liquids, which results in very high manufacturing cost, or very difficult installation due to the communication inside the device through a hose.
In the dyeing and decoloring device in the prior art, a shaking table is required to be used in one process, however, the shaking table and the dyeing and decoloring device are difficult to integrate together, even if some inventions integrate the shaking table together, a wish is only a wish or an assumption cannot be realized at all, and when the shaking table shakes, the shaking table drives a dyeing box and a large number of connected pipelines to shake together, which is not a practical and reliable technical scheme at all.
The dyeing and decoloring device in the prior art has many problems caused by integration, and one problem is that the dyeing box has insufficient space to realize shaking when a shaking table is used.
The dyeing and decoloring device in the prior art only considers the discharge of liquid and does not consider the dispersion of waste gas, and the concentrated waste gas influences the health of operators when being manually opened.
The dyeing and decoloring device in the prior art has no effective flowing power structure when liquid flows, so that the liquid flows unsmoothly.
Disclosure of Invention
In order to overcome the above problems, the present invention proposes a solution to solve the above problems simultaneously.
The technical scheme adopted by the invention for solving the technical problems is as follows: an automatic dyeing and decoloring device comprises a main body, a fixed liquid box, a silver dyeing liquid box, a developing liquid box, a washing liquid box, a partition plate, a switching ring, a dyeing bin, a dyeing box, a connecting shaft, an air dispersing box, a liquid discharging box, a first handle, a through flow cover, an upright post, a supporting leg, an upper cover, a liquid inlet, a supporting plate, an air vent, a groove, a convex shoulder, a shaking table, a switching sleeve, a supporting piece, a control valve, a motor, a connecting rod, an upper clamping ring and a lower clamping ring; wherein the upper cover is provided with an upper hole and a guide plate; the adapter sleeve comprises a liquid discharge ring, a spacer ring, a recess and a second handle;
the main body is internally provided with a supporting plate, four clapboards are arranged above the supporting plate, four spaces separated by the four clapboards are respectively used for accommodating the fixed liquid box, the silver staining liquid box, the developing liquid box and the washing liquid box, the bottom surfaces of the four boxes do not completely cover the supporting plate, the supporting plate is also provided with the adapter ring, the four boxes surround the adapter ring, the inner sides of the four boxes are respectively provided with a liquid inlet, the outer wall of the adapter ring is provided with an opening, and the opening is sequentially communicated with the four liquid inlets through the rotation of the adapter ring; a first handle is arranged above the adapter ring, the through-flow cover is connected to the inner side of the adapter ring, the inner side of the adapter ring is in fluid communication with the inner side of the through-flow cover, an upright post is arranged below the through-flow cover, a support leg is arranged below the upright post, the height of the upper surface of the edge of the through-flow cover is the same as that of the upper surface of the support leg, the support leg is supported on the upper cover, and the guide plate is arranged below the upper hole of the upper cover;
the gas diffusion box is arranged below the supporting plate, surrounds the dyeing bin, is provided with the vent hole on the inner side and is internally provided with the dyeing box; the control valve is arranged below the dyeing box and controls the fluid communication between the inside and the outside of the dyeing box; the dyeing box is supported on the rocking bed, a convex shoulder and a groove are arranged on the upper surface of the rocking bed, and liquid flows into the groove through the convex shoulder after flowing out of the dyeing box;
the periphery of the shaking table surrounds the adapter sleeve, the periphery of the adapter sleeve surrounds the drainage box, the upper part of the adapter sleeve is provided with the spacer ring to separate the drainage ring from the lower part of the adapter sleeve, a recess is arranged below the adapter sleeve, the second handle is arranged in the recess, and the adapter sleeve is driven to move by the second handle, so that the on-off of fluid between the drainage ring and the drainage box is realized;
the circumferential surface of the dyeing box comprises two planes and two cambered surfaces; the inner wall of the dyeing box is provided with a plurality of supporting pieces, and the supporting pieces are distributed on the three height surfaces and used for placing pieces to be dyed;
one part of the lower surface of the adapter ring is supported on the support plate, the other part of the lower surface of the adapter ring is connected with the adapter sleeve through four connecting rods, and the connecting rods are arranged in a cavity between the gas dispersing box and the dyeing bin; the outer surface of the adapter ring is provided with an upper clamping ring which is clamped into the four boxes in a sliding manner, the outer surface of the adapter sleeve is provided with a lower clamping ring which is clamped into the drainage box in a sliding manner; the dyeing machine is characterized in that the motor is arranged above the through-flow cover, the through-flow cover and the switching ring are driven to rotate through the rotation of the motor, the switching sleeve is driven to rotate through the connecting rod, the gas diffusion box can be taken out of the main body, the dyeing bin is provided with an opening, and after the gas diffusion box is taken out, the dyeing box can be placed into the dyeing bin through the opening from a gap of the connecting rod.
Preferably, the drainage box has four drainage boxes.
Preferably, partition plates are arranged among the four liquid discharge boxes.
Preferably, the height of the upper surface of the through-flow cover is the same as the height of the upper surface of the adapter ring.
Preferably, the upper cover is detachably connected with the dyeing bin.
Preferably, the first handle and the second handle are cylindrical structures.
Preferably, the dyeing box is connected with the dyeing bin through a connecting shaft.
Preferably, the connecting shafts are positioned at two sides of the dyeing box.
Preferably, the dyeing box can swing around the connecting shaft.
Preferably, the body is of circular configuration.
The invention has the beneficial effects that:
1. aiming at the 1 st point of the background technology, the integrated design is adopted, a plurality of processes are integrated into one device, and a dyeing box, a shaking table, a liquid storage box, a liquid discharge box, an exhaust box and the like are integrated into one device and operate organically and efficiently.
Point 2 of the background art
1) Through the design of the adapter ring, the adapter ring is sequentially connected with different liquid storage boxes, so that a plurality of groups of passages capable of controlling on-off are constructed, the use of a large number of remote control valves is avoided, only one valve is used in the whole device, and the cost is greatly reduced.
) Through the design of adapter sleeve, adapter sleeve divide into two parts, and one part is the through-flow part, and another part is the rotation part, cuts off each other between the two, and the through-flow part is connected the other end with the recess and is connected with the flowing back box, realizes the circulation break-make of flow path.
The 3 rd point that proposes to the background art, through simple but effectual flow path structure, for example the complete but succinct flow path of constituteing such as guide plate, the inlet of conversion, feed liquor space, avoided a large amount of problems that complicated flow path structure brought.
And the 4 th point that proposes to the background art, through shaking table upper surface design faying face and recess, be connected through faying face and dyeing box, realize the height drop so that the through-flow through the recess, also make the size of faying face and dyeing box more match simultaneously.
And the 5 th point proposed for the background technology, the cross section of the dyeing box is designed to be the combination of two straight lines and two arc lines, so that the space in one direction is reduced, and enough space is reserved for shaking.
And the 6 th point proposed for the background technology, a removable gas diffusion box is designed, and the waste gas is dispersed and taken out for cleaning after the experiment is finished.
And aiming at the 7 th point of the background technology, designs such as a guide plate, a groove and the like are adopted, and gradient difference flow and buffer flow are provided.
Note: the foregoing designs are not sequential, each of which provides a distinct and significant advance in the present invention over the prior art.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a top view of the present invention
FIG. 2 is a front view structural diagram of the present invention
FIG. 3 is a bottom view of the present invention
FIG. 4 is a three-dimensional schematic view of the staining box of the present invention
In the figures, the reference numerals are as follows:
1. the dyeing machine comprises a main body 2, a fixed liquid box 3, a silver dyeing liquid box 4, a developing liquid box 5, a washing liquid box 6, a partition plate 7, an adapter ring 8, a dyeing bin 9, a dyeing box 10, a connecting shaft 11, an air dispersing box 12, a liquid discharging box 13, a first handle 14, a through flow cover 15, an upright post 16, a support leg 17, an upper cover 18, a guide plate 19, a liquid inlet 20, an upper hole 21, a support plate 22, an air vent 23, a groove 24, a shoulder 25, a liquid discharging ring 26, a second handle 27, a shaking table 28, a recess 29, a spacer ring 30, an adapter sleeve 31, a plane 32, an arc surface 33, a support piece 34, a control valve 35, a connecting rod 36, a motor 37, an upper clamping ring.
Detailed Description
As shown in the figure: an automatic dyeing and decoloring device comprises a main body, a fixed liquid box, a silver dyeing liquid box, a developing liquid box, a washing liquid box, a partition plate, a switching ring, a dyeing bin, a dyeing box, a connecting shaft, an air dispersing box, a liquid discharging box, a first handle, a through flow cover, an upright post, a supporting leg, an upper cover, a liquid inlet, a supporting plate, an air vent, a groove, a convex shoulder, a shaking table, a switching sleeve, a supporting piece, a control valve, a motor, a connecting rod, an upper clamping ring and a lower clamping ring; wherein the upper cover is provided with an upper hole and a guide plate; the adapter sleeve comprises a liquid discharge ring, a spacer ring, a recess and a second handle;
the main body is internally provided with a supporting plate, four clapboards are arranged above the supporting plate, four spaces separated by the four clapboards are respectively used for accommodating the fixed liquid box, the silver staining liquid box, the developing liquid box and the washing liquid box, the bottom surfaces of the four boxes do not completely cover the supporting plate, the supporting plate is also provided with the adapter ring, the four boxes surround the adapter ring, the inner sides of the four boxes are respectively provided with a liquid inlet, the outer wall of the adapter ring is provided with an opening, and the opening is sequentially communicated with the four liquid inlets through the rotation of the adapter ring; a first handle is arranged above the adapter ring, the through-flow cover is connected to the inner side of the adapter ring, the inner side of the adapter ring is in fluid communication with the inner side of the through-flow cover, an upright post is arranged below the through-flow cover, a support leg is arranged below the upright post, the height of the upper surface of the edge of the through-flow cover is the same as that of the upper surface of the support leg, the support leg is supported on the upper cover, and the guide plate is arranged below the upper hole of the upper cover;
as shown in the figure: the gas diffusion box is arranged below the supporting plate, surrounds the dyeing bin, is provided with the vent hole on the inner side and is internally provided with the dyeing box; the control valve is arranged below the dyeing box and controls the fluid communication between the inside and the outside of the dyeing box; the dyeing box is supported on the rocking bed, a convex shoulder and a groove are arranged on the upper surface of the rocking bed, and liquid flows into the groove through the convex shoulder after flowing out of the dyeing box;
the periphery of the shaking table surrounds the adapter sleeve, the periphery of the adapter sleeve surrounds the drainage box, the upper part of the adapter sleeve is provided with the spacer ring to separate the drainage ring from the lower part of the adapter sleeve, a recess is arranged below the adapter sleeve, the second handle is arranged in the recess, and the adapter sleeve is driven to move by the second handle, so that the on-off of fluid between the drainage ring and the drainage box is realized;
the circumferential surface of the dyeing box comprises two planes and two cambered surfaces; the inner wall of the dyeing box is provided with a plurality of supporting pieces, and the supporting pieces are distributed on the three height surfaces and used for placing pieces to be dyed;
as shown in the figure: one part of the lower surface of the adapter ring is supported on the support plate, the other part of the lower surface of the adapter ring is connected with the adapter sleeve through four connecting rods, and the connecting rods are arranged in a cavity between the gas dispersing box and the dyeing bin; the outer surface of the adapter ring is provided with an upper clamping ring which is clamped into the four boxes in a sliding manner, the outer surface of the adapter sleeve is provided with a lower clamping ring which is clamped into the drainage box in a sliding manner; the dyeing machine is characterized in that the motor is arranged above the through-flow cover, the through-flow cover and the switching ring are driven to rotate through the rotation of the motor, the switching sleeve is driven to rotate through the connecting rod, the gas diffusion box can be taken out of the main body, the dyeing bin is provided with an opening, and after the gas diffusion box is taken out, the dyeing box can be placed into the dyeing bin through the opening from a gap of the connecting rod.
As shown in the figure: the drainage box has four. And partition plates are arranged among the four liquid discharge boxes. The height of the upper surface of the through-flow cover is the same as that of the upper surface of the adapter ring. The upper cover is detachably connected with the dyeing bin. The first handle and the second handle are of cylindrical structures. The dyeing box is connected with the dyeing bin through a connecting shaft. The connecting shafts are positioned on two sides of the dyeing box. The dyeing box can swing around the connecting shaft. The main body is of a circular structure.
The working principle is as follows: the supporting pieces are perpendicular to the circumferential surface of the dyeing box in a working state, the supporting pieces can rotate upwards to expand the internal space of the dyeing box, and three pieces of pieces to be dyed are placed corresponding to three height surfaces and are supported on the corresponding supporting pieces; taking the gas diffusion box out of the main body, placing the dyeing box into a dyeing bin through the opening from the gap of the four connecting rods, overlapping the guide plate on the dyeing box due to the action of gravity, and finally placing the gas diffusion box back to the main body;
the liquid feeding method comprises the following steps: the motor is operated to rotate to drive the through-flow cover and the adapter ring to rotate, so that the through ports on the adapter ring are sequentially communicated with the four liquid inlets, different liquids are sequentially introduced into the dyeing box, and the liquids sequentially flow through the liquid inlets, the through ports, the through-flow cover, the upper hole and the guide plate to enter the dyeing box;
the liquid discharging method comprises the following steps: the motor drives the adapter ring to rotate, and simultaneously drives the adapter sleeve to synchronously rotate through the connecting rod, so that the liquid discharge ring is communicated with the liquid discharge box; secondly, controlling the control valve to be opened, and enabling liquid in the dyeing box to flow through the convex shoulder, the groove and the liquid discharge ring in sequence to enter the liquid discharge box;
the exhaust method comprises the following steps: after the control valve is controlled to be opened, waste gas enters the air dispersing box through the opening and the vent hole, and the air dispersing box is taken out from the main body for cleaning after the work is finished.
The above detailed description is specific to possible embodiments of the present invention, and the embodiments are not intended to limit the scope of the present invention, and all equivalent implementations or modifications that do not depart from the scope of the present invention are intended to be included within the scope of the present invention.

Claims (10)

1. The utility model provides an automatic change dyeing decoloration device which characterized in that: the dyeing machine comprises a main body, a fixed liquid box, a silver dyeing liquid box, a developing liquid box, a washing liquid box, a partition plate, a switching ring, a dyeing bin, a dyeing box, a connecting shaft, an air diffusing box, a liquid discharging box, a first handle, a through flow cover, an upright post, a support leg, an upper cover, a liquid inlet, a support plate, an air vent, a groove, a convex shoulder, a table, a switching sleeve, a support piece, a control valve, a motor, a connecting rod, an upper clamping ring and a lower clamping ring; wherein the upper cover is provided with an upper hole and a guide plate; the adapter sleeve comprises a liquid discharge ring, a spacer ring, a recess and a second handle;
the main body is internally provided with a supporting plate, four clapboards are arranged above the supporting plate, four spaces separated by the four clapboards are respectively used for accommodating the fixed liquid box, the silver staining liquid box, the developing liquid box and the washing liquid box, the bottom surfaces of the four boxes do not completely cover the supporting plate, the supporting plate is also provided with the adapter ring, the four boxes surround the adapter ring, the inner sides of the four boxes are respectively provided with a liquid inlet, the outer wall of the adapter ring is provided with an opening, and the opening is sequentially communicated with the four liquid inlets through the rotation of the adapter ring; a first handle is arranged above the adapter ring, the through-flow cover is connected to the inner side of the adapter ring, the inner side of the adapter ring is in fluid communication with the inner side of the through-flow cover, an upright post is arranged below the through-flow cover, a support leg is arranged below the upright post, the height of the upper surface of the edge of the through-flow cover is the same as that of the upper surface of the support leg, the support leg is supported on the upper cover, and the guide plate is arranged below the upper hole of the upper cover;
the gas diffusion box is arranged below the supporting plate, surrounds the dyeing bin, is provided with the vent hole on the inner side and is internally provided with the dyeing box; the control valve is arranged below the dyeing box and controls the fluid communication between the inside and the outside of the dyeing box; the dyeing box is supported on the rocking bed, a convex shoulder and a groove are arranged on the upper surface of the rocking bed, and liquid flows into the groove through the convex shoulder after flowing out of the dyeing box;
the periphery of the shaking table surrounds the adapter sleeve, the periphery of the adapter sleeve surrounds the drainage box, the upper part of the adapter sleeve is provided with the spacer ring to separate the drainage ring from the lower part of the adapter sleeve, a recess is arranged below the adapter sleeve, the second handle is arranged in the recess, and the adapter sleeve is driven to move by the second handle, so that the on-off of fluid between the drainage ring and the drainage box is realized;
the circumferential surface of the dyeing box comprises two planes and two cambered surfaces; the inner wall of the dyeing box is provided with a plurality of supporting pieces, and the supporting pieces are distributed on the three height surfaces and used for placing pieces to be dyed;
one part of the lower surface of the adapter ring is supported on the support plate, the other part of the lower surface of the adapter ring is connected with the adapter sleeve through four connecting rods, and the connecting rods are arranged in a cavity between the gas dispersing box and the dyeing bin; the outer surface of the adapter ring is provided with an upper clamping ring which is clamped into the four boxes in a sliding manner, the outer surface of the adapter sleeve is provided with a lower clamping ring which is clamped into the drainage box in a sliding manner; the dyeing machine is characterized in that the motor is arranged above the through-flow cover, the through-flow cover and the switching ring are driven to rotate through the rotation of the motor, the switching sleeve is driven to rotate through the connecting rod, the gas diffusion box can be taken out of the main body, the dyeing bin is provided with an opening, and after the gas diffusion box is taken out, the dyeing box can be placed into the dyeing bin through the opening from a gap of the connecting rod.
2. The automatic dyeing and decoloring device according to claim 1, wherein: the drainage box has four.
3. The automatic dyeing and decoloring device according to claim 2, wherein: and partition plates are arranged among the four liquid discharge boxes.
4. The automatic dyeing and decoloring device according to claim 1, wherein: the height of the upper surface of the through-flow cover is the same as that of the upper surface of the adapter ring.
5. The automatic dyeing and decoloring device according to claim 1, wherein: the upper cover is detachably connected with the dyeing bin.
6. The automatic dyeing and decoloring device according to claim 1, wherein: the first handle and the second handle are of cylindrical structures.
7. The automatic dyeing and decoloring device according to claim 1, wherein: the dyeing box is connected with the dyeing bin through a connecting shaft.
8. The automatic dyeing and decoloring device according to claim 7, wherein: the connecting shafts are positioned on two sides of the dyeing box.
9. The automatic dyeing and decoloring device according to claim 7, wherein: the dyeing box can swing around the connecting shaft.
10. The automatic dyeing and decoloring device according to claim 1, wherein: the main body is of a circular structure.
CN202010165865.9A 2020-03-11 2020-03-11 Automatic change dyeing decoloration device Active CN111323285B (en)

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