CN106771288A - A kind of Mycobacterium tuberculosis automatic checkout equipment - Google Patents

A kind of Mycobacterium tuberculosis automatic checkout equipment Download PDF

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Publication number
CN106771288A
CN106771288A CN201710045486.4A CN201710045486A CN106771288A CN 106771288 A CN106771288 A CN 106771288A CN 201710045486 A CN201710045486 A CN 201710045486A CN 106771288 A CN106771288 A CN 106771288A
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CN
China
Prior art keywords
fuselage
cylinder
plate
slide
alcohol
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Granted
Application number
CN201710045486.4A
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Chinese (zh)
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CN106771288B (en
Inventor
王晓春
郭冬梅
王建
许礼发
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Anhui University of Science and Technology
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Anhui University of Science and Technology
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Priority to CN201710045486.4A priority Critical patent/CN106771288B/en
Publication of CN106771288A publication Critical patent/CN106771288A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44Sample treatment involving radiation, e.g. heat
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099Characterised by type of test elements
    • G01N2035/00138Slides

Abstract

The present invention relates to medical machinery field, specifically a kind of Mycobacterium tuberculosis automatic checkout equipment, including fuselage, rotary vane device, smear sputum device, dyeing apparatus and flusher;PLC is installed, described rotary vane device is located at waist on described fuselage, smears sputum device positioned at fuselage left part, dyeing apparatus is located at fore-body, and flusher is located at fuselage right part:A kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, during it is detected to mycobacterium tuberculosis, the automation mechanized operation realize and apply phlegm film, dyeed, decolourize and redye process, reduces the time that medical worker contacts germ, is beneficial to the healthy of medical personnel.

Description

A kind of Mycobacterium tuberculosis automatic checkout equipment
Technical field
The present invention relates to medical machinery field, specifically a kind of Mycobacterium tuberculosis automatic checkout equipment.
Background technology
Mycobacterium tuberculosis (M.tuberculosis), is commonly called as tubercle bacillus, is to cause pathogen lungy.Can invade Each organ of whole body, but with pulmonary tuberculosis as most common.Tuberculosis is still important infectious disease so far.According to WHO, there are about every year 8000000 new cases occur, and at least 3,000,000 people die from the disease.
Mycobacterium tuberculosis is the elongated bacillus for slightly bending, 1~4X0.4 μm of size.Mycobacterium bovis then compares tubbiness. The bacteria cell wall lipid content of Mycobacterium is higher, accounts for the 60% of dry weight, particularly there is a large amount of mycolic acids (mycolic acid) is enclosed in the outside of peptidoglycan layer, can influence penetrating for dyestuff.Mycobacteria is typically with neat Buddhist nun (Ziehl- Neelsen) Ziehi-Neelsen stain, can be caught after the heating dyeing of 5% carbolfuchsin, but is difficult to decolourize with 3% acidic alcohol. If being redyed again plus with methylene blue, mycobacteria takes on a red color, and the material in other bacteriums and background is blueness.
Existing Mycobacterium tuberculosis test experience is:
1st, experiment equipment:Sputum bottle, first dye liquor, decolorising agent, redye liquid, bamboo stick, slide, alcolhol burner, staining rack, heating rod, Slide conserving case, microscope and mirror oil etc.
2nd, experimental procedure:
(1) patient's sputum is gathered, is collected into sputum bottle;
(2) sequence number is marked on slide with pencil or glass icking tool;
(3) mark on verification sputum bottle and laboratory test report;
(4) choose phlegm with bamboo stick to smear on slide, uniform application ovalisation;
(5) slide stands, and spontaneously dries, and is placed on staining rack after drying;
(6) heating rod is placed in alcoholic solution, then heating rod is lighted with alcolhol burner;
(7) just dye liquor, the full slide phlegm film of lid are added;
(8) just dye liquor is heated to slide with heating rod and steam occurs, then slide is departed from flame, keep dyeing 5min;
(9) light and slow current rinse slide, then add decolorising agent, are cleaned with water to redfree;
(10) add and redye liquid, dye 30s, then rinsed with clear water;
(11) determine whether the thickness that phlegm film is smeared is qualified by the light transmittance of phlegm film;
(12) the upper mirror oil of slide drop after will be qualified, then the number with micro- sem observation mycobacterium tuberculosis within the specific limits Amount.
Above-mentioned experimentation is the existing general detecting step of mycobacterium tuberculosis, and in experimentation, people will be for a long time Contact mycobacterium tuberculosis, hidden danger is brought to the safety of medical worker, and in the prior art, there has been no one kind can be right Mycobacterium tuberculosis avoids manually-operated automation equipment as far as possible during being detected, in consideration of it, the invention provides A kind of experimental facilities, it has the advantages that:
(1) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, it can be realized by smearing sputum device It is automatic on slide to smear phlegm film;
(2) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, it can be realized by dyeing apparatus Operation is heated, decolourizes and redyes in automatic just dye, the alcohol of carrying out of the slide for having smeared phlegm film;
(3) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, it can be realized by flusher Automatic cleaning to liquid on slide in dyeing course;
(4) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, four pieces of swivel plates on its rotary vane device Design is rotated so that the present invention can carry out continual operation, when reducing the stop between the different sputum samples of detection Between, improve efficiency;
(5) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, the swingable design of its swivel plate is caused Just dye liquor, decolorising agent of the invention, redye liquid and clear water and can more easily be recovered;
(6) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, it disposably at least can be to 9 samples Detected, detection efficiency is high;
(7) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, its mistake to mycobacterium tuberculosis detection Cheng Zhong, the automation mechanized operation realize and apply phlegm film, dyeed, decolourize and redye process, reduce medical worker contact germ when Between, it is beneficial to the healthy of medical personnel.
The content of the invention
In order to make up the deficiencies in the prior art, a kind of heretofore described Mycobacterium tuberculosis automatic checkout equipment, its Can realize applying phlegm film, dye, decolourize and redye the automation mechanized operation of process during being detected to mycobacterium tuberculosis.
The technical solution adopted for the present invention to solve the technical problems is:A kind of Mycobacterium tuberculosis automatic checkout equipment, Including fuselage, rotary vane device, smear sputum device, dyeing apparatus and flusher;PLC, PLC are installed on described fuselage Controller is used to control the automatic job of whole electrical equipment of the invention and pneumatic equipment, and described rotary vane device is located in fuselage Portion, smears sputum device positioned at fuselage left part, and dyeing apparatus is located at fore-body, and flusher is located at fuselage right part:
Described rotary vane device is used to place slide, and to rotate and smear sputum device, dyeing so that slide to be sequentially passed through Device and flusher;
Described smears sputum device for disposing sputum bottle, and by the phlegm uniform application in sputum bottle on slide;
Described dyeing apparatus is used to that the slide for wiping phlegm to be carried out just to contaminate, decolourize and redye;
Slide during described flusher is used for just contaminating, decolourizing and redye carries out clear water flushing.
Described rotary vane device includes electronic rotation disk, swivel plate and oscillating cylinder;Described swivel plate quantity is four, four Individual swivel plate is flat cube plate, and four swivel plates are uniform equally spaced horizontally hinged on electronic rotation disk top;Respectively At least three row's hollow out groups are evenly arranged with swivel plate, at least three hollow out film perforations are evenly arranged with each row's hollow out group, respectively engraved Empty film perforation surrounding is provided with square stop frame, and slide can just be embedded in stop inframe, each to stop that outer frame end is provided with leak-off chute;Institute The oscillating cylinder stated is evenly arranged in electronic rotation disk surrounding, and oscillating cylinder bottom is hinged on electronic rotation disk, swings gas Cylinder top is hinged on swivel plate.
Under original state, each swivel plate is in horizontality, during work, first, clean slide is placed on into four rotations In each stop frame in flap on last sidespin flap, then, electronic rotation disk rotates to each swivel plate so that carry glass The swivel plate of piece is turned to successively to be smeared at sputum device, dyeing apparatus and flusher;Meanwhile, during dyeing and flushing, Liquid will be stayed on slide, now, can shrink oscillating cylinder, so that swivel plate is rotated down, and then be caused on slide Liquid from stop frame each leak-off chute in flow out;Four swivel plates repeat the above-mentioned course of work in turn successively.
Described sputum device of smearing includes shelf, puts bottle plate and Tu Tan mechanisms;Described shelf quantity is two, and two are put Thing plate is symmetrically vertically installed at fuselage left part, and two shelf lower ends are provided with the flange for extending internally;
Described puts bottle plate for flat square plate, puts bottle plate and can be placed on two flanges of shelf, puts on bottle plate The even hollow cylinder that is provided with puts a bottle storehouse, put put on bottle plate bottle storehouse quantity it is equal with the quantity of hollow out film perforation on each swivel plate, Put a bottle storehouse inner side and be surrounded with elastic sheet rubber;Respectively put a bottle storehouse right-hand member and be provided with square painting phlegm groove, a bottle plate is put when swivel plate is located at During underface, a painting phlegm groove is correspond to directly over each hollow out film perforation;
Described Tu Tan mechanisms include dynamic plate cylinder, apply the dynamic plate of phlegm, apply mental disorder cylinder and contiguous block, move plate cylinder from left to right It is horizontally arranged on the fuselage directly over shelf, the dynamic plate level of described painting phlegm is arranged on dynamic plate cylinder, applies under the dynamic plate of phlegm End face vertical is provided with painting mental disorder cylinder, applies the quantity of mental disorder cylinder and to put quantity that bottle storehouse is respectively put on bottle plate equal, and respectively put a bottle storehouse Surface corresponds to a painting mental disorder cylinder;Each phlegm cylinder bottom that applies is fixed with contiguous block, and contiguous block middle part is provided with cylinder Elastic rubber column, elastic rubber column middle part is provided with elongated cylindrical inserted link hole, and the design of elastic rubber column is easy to bamboo stick to insert In inserted link hole.
During work, the bamboo for dipping sputum will be plugged in each inserted link hole of the elastic rubber column of each painting mental disorder cylinder first Sign, when needing to detect different sputums, then the sputum bottle that will be equipped with different sputums is put into respectively the putting in bottle storehouse of bottle plate, and It is quick to open each phlegm bottle cap mouth, then quickly will be equipped with bottle plate of putting of sputum bottle and be put into shelf, then, the work of Tu Tan mechanisms, Dynamic plate cylinder drive applies the dynamic plate motion of phlegm, to cause that each painting mental disorder cylinder is placed exactly in the surface of sputum bottle opening, then, applies mental disorder Cylinder stretches out so that the bamboo stick being connected with contiguous block dips in upper sputum, then, then by the motion of Tu Tan mechanisms so that dip in full sputum Bamboo stick be removed from sputum bottle and extend into bamboo stick and apply immediately below phlegm groove on the slide of hollow out film perforation so that in different sputum bottles Sputum can be applied to respectively on the different slides of swivel plate;After completing above-mentioned steps, manually sputum bottle and each bamboo stick are carried out With changing, phlegm operation is smeared with carry out slide on next swivel plate.
Described dyeing apparatus includes heater and dropper;Described heater integral installation is before fuselage Portion, and heater includes that alcohol spray groove, alcohol nozzle, alcohol go out groove, hot pin cylinder, heating rod, flame groove, door and moves lid Cylinder;Alcohol spray groove is arranged on fuselage right part upper end, and alcohol spray groove lower end is uniformly provided with the spray of at least three alcohol from front to back Mouth, alcohol goes out groove immediately below alcohol nozzle, and alcohol goes out groove is used for the alcohol that recovery section is sprayed from alcohol nozzle;
Described hot pin cylinder is horizontally arranged at underbelly from left to right, and heating rod is arranged on hot pin gas by cylinder block At least three heating heads are provided with cylinder, and on heating rod, when heating rod is located at immediately below the hollow out film perforation of swivel plate, are respectively engraved A heating head is corresponded to immediately below empty film perforation, when heating rod is located at immediately below alcohol nozzle, immediately below each alcohol nozzle One heating head of correspondence;
Described flame groove is arranged on fuselage left part, and flame groove is provided with electronics and beats built with alcohol, and flame groove sidewall Firearm, is horizontally installed with shifting lid cylinder from left to right on the fuselage above flame groove, move and flat square gear is provided with lid cylinder Lid, when door is located at directly over flame groove, door completely encloses flame groove.
During work, when the slide that sputum has been spread in painting is together moved at dyeing apparatus with swivel plate, and need to slide When being heated, then heater work, hot pin cylinder drives heating rod to move to immediately below alcohol spray groove, and alcohol spray groove passes through Alcohol nozzle to three heating head sprinkling alcohol of heating rod, in the presence of hot pin cylinder transport again in sprinkling by the heating rod of alcohol Flame groove is moved, now, the door of flame groove upper end departs from flame groove, then, electronic lighter in the presence of lid cylinder is moved Strike sparks to cause the alcohol burn in flame groove, so as to light the heating rod above flame groove;Then door is reclosed Flame groove, the heating rod of burning is moved to below each slide of swivel plate successively under the drive of hot pin cylinder, so as to each glass Piece is heated.
Described dropper includes dropping liquid cylinder, dropping liquid slide plate, storage chamber, No. two storage chambers, No. three storage chambers, a dropping liquids Pipe, liquid-dropping valve, dropping liquid nozzle and dropping liquid collection box, described dropping liquid cylinder are arranged on back, and dropping liquid slide plate is arranged on drop On liquid cylinder, a storage chamber, No. two storage chambers and No. three storage chambers are installed in dropping liquid slide plate upper surface, and storage chamber, No. two storages Chamber is connected with row's dropping liquid nozzle with No. three storage chamber lower ends each via minim pipette, and the quantity of each row's dropping liquid nozzle and position are equal Hollow out film perforation with each row's hollow out group on swivel plate is corresponding, and described liquid-dropping valve is arranged on minim pipette, and described dropping liquid is returned Case is received to be arranged on fuselage and be located at dropping liquid nozzle lower right, dropping liquid collection box be used to reclaiming from swivel plate updip fall down it is first Dye liquor, decolorising agent and redye liquid.Just dye liquor, de- is sequentially arranged with a described storage chamber, No. two storage chambers and No. three storage chambers respectively Toner and redye liquid.
, it is necessary to first first dye liquor, now, dropper work will be added on slide before heater is heated to slide Make, dropping liquid cylinder drives dropping liquid slide plate to move forward and backward, and dropping liquid skateboarding drives each dropping liquid nozzle being connected with a storage chamber Each slide on swivel plate is sequentially passed through from front to back, during corresponding each dropping liquid nozzle sequentially passes through each slide, one First dye liquor in number storage chamber is flowed in dropping liquid nozzle in the presence of liquid-dropping valve from minim pipette, then flows out to slide from dropping liquid nozzle On so that the sputum coating on just dye liquor covering slide, the first dye liquor on slide is arranged by the inclination of swivel plate again then Go out, and slide is together turned into flusher with swivel plate and cleaned so that the first dye liquor on slide will be remained in, will then wash again Net slide is gone back at dyeing apparatus, repeats above-mentioned identical step, using the decolorising agent in No. two storage chambers and No. three storage chambers Slide is decolourized successively and is redyed treatment with liquid is redyed;Wherein, slide will up to be covered first dye liquor is covered into slide First dye liquor rotate to the interstage of flusher, it is necessary to be heated by heating rod to slide, be heated on slide Untill there is steam in first dye liquor.
Described flusher includes flushing tank, flush pipe, flushing valve and sewerage recovering box, and described flushing tank is arranged on Fuselage right part upper end, flushing tank lower end sets and is communicated with flush pipe, the quantity of hollow out film perforation in the quantity and swivel plate of flush pipe It is equal, and when swivel plate is located at immediately below flusher, it is corresponding directly over each hollow out film perforation to be disposed with a flush pipe, respectively Flushing valve is mounted in flush pipe, described sewerage recovering box is arranged on fuselage and positioned at flushing tank lower rear.
During work, the slide for covering just dye liquor, decolorising agent and redying liquid turns to flusher from dyeing apparatus, rinses dress Work is put, flushing valve is opened, and the clear water in flushing tank is flowed out on slide from flush pipe, cleaned with to slide, wherein, quilt The sewage for rinsing is flowed obliquely into sewerage recovering box by swivel plate.
Specifically, as a kind of preferred technical scheme of the invention, flowing flux is additionally provided with described swivel plate, hinder Gear frame is connected with flowing flux along being extended out at leak-off chute, described flowing flux right-hand member relative to flowing flux left end closer to Fuselage outer side, the design causes that liquid flows out from the side of flowing flux all the time, consequently facilitating the centralized recovery of liquid;From stop frame Leak-off chute liquid out swivel plate is flowed out by flowing flux.
Specifically, as a kind of preferred technical scheme of the invention, the fuselage left side is provided with and can will put a bottle plate and enter Enter shelf and the opening of the bamboo stick insertion Tu Tan mechanisms of phlegm will be applied;Fuselage front side is provided with and is available for alcohol to go out groove and dropping liquid The opening of liquid outflow in collection box, and the woven hose that alcohol can be added to flame groove;Fuselage rear side is provided with and is available for sewage The opening of sewage outflow in collection box, and it is put into swivel plate and the access aperture from swivel plate taking-up for slide;Back Being provided with can spray the woven hose that liquid is added in grooves and flushed channels to a storage chamber, No. two storage chambers, No. three storage chambers, alcohol respectively; Other positions of fuselage are in sealing state;The purpose of above-mentioned design is to prevent the diffusion of bacterium, reduces outworker's Work risk.
Specifically, as a kind of preferred technical scheme of the invention, each painting mental disorder cylinder lower end of Tu Tan mechanisms can be with The electromagnet of one monoblock flat square of common connection, the adsorbable flat iron block in electromagnet lower section, iron block lower surface is provided with Contiguous block, can be inserted into bamboo stick in each contiguous block, so as to every time with changing during bamboo stick, need to only power off electromagnet, make flat iron block whole Block is taken out, and concentration replacing is carried out to the bamboo stick on flat iron block, after more finishing changing, then iron block is placed on into electromagnet lower section, is made Electromagnet is obtained to be powered to adsorbing iron block;So as to avoid people by hand stretch into apply mental disorder cylinder bottom bamboo stick is carried out one by one with The trouble changed and bring, while reducing the possibility that operating personnel are infected by bacterial.
Beneficial effect:
(1) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, it can be realized by smearing sputum device It is automatic on slide to smear phlegm film;
(2) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, it can be realized by dyeing apparatus Operation is heated, decolourizes and redyes in automatic just dye, the alcohol of carrying out of the slide for having smeared phlegm film;
(3) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, it can be realized by flusher Automatic cleaning to liquid on slide in dyeing course;
(4) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, four pieces of swivel plates on its rotary vane device Design is rotated so that the present invention can carry out continual operation, when reducing the stop between the different sputum samples of detection Between, improve efficiency;
(5) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, the swingable design of its swivel plate is caused Just dye liquor, decolorising agent of the invention, redye liquid and clear water and can more easily be recovered;
(6) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, it disposably at least can be to 9 samples Detected, detection efficiency is high;
(7) a kind of Mycobacterium tuberculosis automatic checkout equipment that the present invention is provided, its mistake to mycobacterium tuberculosis detection Cheng Zhong, the automation mechanized operation realize and apply phlegm film, dyeed, decolourize and redye process, reduce medical worker contact germ when Between, it is beneficial to the healthy of medical personnel.
Brief description of the drawings
The present invention is further described with implementation method below in conjunction with the accompanying drawings.
Fig. 1 is overall schematic of the invention;
Fig. 2 is another visual angle schematic diagram of Fig. 1;
Fig. 3 is the structural representation of rotary vane device of the present invention;
Fig. 4 is the upward view of rotary vane device of the present invention;
Fig. 5 is the top view of swivel plate of the present invention;
Fig. 6 is the structural representation of swivel plate of the present invention;
Fig. 7 is the structural representation that the present invention smears sputum device;
Fig. 8 is that the present invention smears the schematic diagram that sputum device is in contact with swivel plate;
Fig. 9 is the schematic diagram at the contiguous block of present invention painting mental disorder cylinder lower end;
Figure 10 is the structural representation of dyeing apparatus of the present invention;
Figure 11 is another visual angle schematic diagram of Figure 10;
Figure 12 is the front view of Figure 10;
Figure 13 is the sketch of heating rod of the present invention;
In figure:Fuselage 1, rotary vane device 2, smear sputum device 3, dyeing apparatus 4, flusher 5, electronic rotation disk 21, rotation Plate 22, oscillating cylinder 23, hollow out group 221, hollow out film perforation 222, stop frame 224, flowing flux 225, shelf 31, put bottle plate 32, Tu Tan mechanisms 33, put bottle storehouse 321, apply phlegm groove 322, dynamic plate cylinder 331, apply the dynamic plate 332 of phlegm, apply mental disorder cylinder 333, contiguous block 334, Rubber column 335, heater 41, dropper 42, alcohol spray groove 411, alcohol nozzle 412, alcohol go out groove 413, hot pin cylinder 414th, heating rod 415, flame groove 416, door 417, shifting lid cylinder 418, heating first 4155, dropping liquid cylinder 421, dropping liquid slide plate 422nd, the storage chamber 424, three of storage chamber 423, two storage chamber 425, minim pipette 426, dropping liquid nozzle 427, dropping liquid collection box 428, Flushing tank 51, flush pipe 52, sewerage recovering box 53.
Specific embodiment
In order that technological means, creation characteristic, reached purpose and effect that the present invention is realized are easy to understand, tie below Implementation method is closed, the present invention is expanded on further.
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7, Fig. 8, Fig. 9, Figure 10, Figure 11, Figure 12 and Figure 13, the present invention A kind of described Mycobacterium tuberculosis automatic checkout equipment, including fuselage 1, rotary vane device 2, smear sputum device 3, the and of dyeing apparatus 4 Flusher 5;PLC is installed on described fuselage 1, PLC be used to controlling whole electrical equipments of the invention and The automatic job of pneumatic equipment, described rotary vane device 2 is located at the middle part of fuselage 1, smears sputum device 3 positioned at the left part of fuselage 1, dyeing dress Put 4 and be located at the front portion of fuselage 1, flusher 5 is located at the right part of fuselage 1:
Described rotary vane device 2 is used to place slide, and to rotate and smear sputum device 3, dye so that slide to be sequentially passed through Color device 4 and flusher 5;
Described smears sputum device 3 for disposing sputum bottle, and by the phlegm uniform application in sputum bottle on slide;
Described dyeing apparatus 4 is used to that the slide for wiping phlegm to be carried out just to contaminate, decolourize and redye;
Slide during described flusher 5 is used for just contaminating, decolourizing and redye carries out clear water flushing.
Described rotary vane device 2 includes electronic rotation disk 21, swivel plate 22 and oscillating cylinder 23;Described swivel plate 22 is counted It is four to measure, and four swivel plates 22 are flat cube plate, and four swivel plates 22 are uniform equally spaced horizontally hinged electronic The top of rotating disk 21;At least three row's hollow out groups 221 are evenly arranged with each swivel plate 22, are uniformly arranged in each row's hollow out group 221 There are at least three hollow out film perforations 222, each surrounding of hollow out film perforation 222 is provided with square stop frame 224, and slide can just be embedded in stop It is each to stop that the outer end of frame 224 is provided with leak-off chute in frame 224;Described oscillating cylinder 23 is evenly arranged in electronic rotation disk 21 4 Week, and the bottom of oscillating cylinder 23 is hinged on electronic rotation disk 21, the top of oscillating cylinder 23 is hinged on swivel plate 22.
Under original state, each swivel plate 22 is in horizontality, during work, first, clean slide is placed on into four In each stop frame 224 in swivel plate 22 on last sidespin flap 22, then, 21 pairs of each swivel plates 22 of electronic rotation disk revolve Turn so that the swivel plate 22 of carrying slide is turned to successively to be smeared at sputum device 3, dyeing apparatus 4 and flusher 5;Meanwhile, in dye Color and rinse during, liquid will be stayed on slide, now, can shrink oscillating cylinder 23 so that swivel plate 22 to Lower rotation, and then cause that the liquid on slide flows out from each leak-off chute for stopping frame 224;Four swivel plates 22 are weighed in turn successively The multiple above-mentioned course of work.
Described sputum device 3 of smearing includes shelf 31, puts bottle plate 32 and Tu Tan mechanisms 33;The described quantity of shelf 31 is Two, two shelfs 31 are symmetrical to be vertically installed at the left part of fuselage 1, and two lower ends of shelf 31 are provided with extend internally convex Edge;
Described puts bottle plate 32 for flat square plate, puts bottle plate 32 and can be placed on two flanges of shelf 31, puts bottle Hollow cylinder is evenly arranged with plate 32 and puts bottle storehouse 321, put to be put on bottle plate 32 and engraved on the quantity in bottle storehouse 321 and each swivel plate 22 The quantity of empty film perforation 222 is equal, puts the inner side of bottle storehouse 321 and is surrounded with elastic sheet rubber;Respectively put the right-hand member of bottle storehouse 321 be provided with it is square Phlegm groove 322 is applied, when swivel plate 22 is located at puts bottle 32 underface of plate, a painting phlegm is correspond to directly over each hollow out film perforation 222 Groove 322;
Described Tu Tan mechanisms 33 include dynamic plate cylinder 331, apply the dynamic plate 332 of phlegm, apply mental disorder cylinder 333 and contiguous block 334, move Plate cylinder 331 is horizontally arranged on the fuselage 1 directly over shelf 31 from left to right, and the dynamic plate 332 of described painting phlegm is horizontally mounted On dynamic plate cylinder 331, the dynamic lower surface of plate 332 of painting phlegm is vertically-mounted painting mental disorder cylinder 333, applies the quantity of mental disorder cylinder 333 and puts The quantity that bottle storehouse 321 is respectively put on bottle plate 32 is equal, and respectively puts corresponding painting mental disorder cylinder 333 directly over bottle storehouse 321;It is each to apply phlegm The bottom of cylinder 333 is fixed with contiguous block 334, and the middle part of contiguous block 334 is provided with Elastic Cylindrical rubber column 335, elastic caoutchouc The middle part of post 335 is provided with elongated cylindrical inserted link hole.
During work, will be plugged for dipping phlegm in each inserted link hole of the elastic rubber column 335 of each painting mental disorder cylinder 333 first The bamboo stick of liquid, when needing to detect different sputums, then the sputum bottle that will be equipped with different sputums is put into respectively putting for bottle plate 32 In bottle storehouse 321, and it is quick each phlegm bottle cap mouth is opened, then quickly will be equipped with bottle plate 32 of putting of sputum bottle and be put into shelf 31, connect , Tu Tan mechanisms 33 work, dynamic plate cylinder 331 drives the dynamic plate 332 of painting phlegm to move, to cause that each painting mental disorder cylinder 333 is placed exactly in The surface of sputum bottle opening, then, applies mental disorder cylinder 333 and stretches out so that the bamboo stick being connected with contiguous block 334 dips in upper sputum, then, Again by the motion of Tu Tan mechanisms 33 so that the bamboo stick for dipping in full sputum is removed from sputum bottle and bamboo stick is extend into painting phlegm groove On the slide of 322 underface hollow out film perforations 222 so that the sputum in different sputum bottles can respectively be applied to the difference of swivel plate 22 On slide;After completing above-mentioned steps, manually sputum bottle and each bamboo stick are carried out with changing, to carry out slide on next swivel plate 22 Smear phlegm operation.
Described dyeing apparatus 4 includes heater 41 and dropper 42;The described integral installation of heater 41 exists Fuselage 1 is anterior, and heater 41 includes that alcohol spray groove 411, alcohol nozzle 412, alcohol go out groove 413, hot pin cylinder 414, add Hot pin 415, flame groove 416, door 417 and shifting lid cylinder 418;Alcohol spray groove 411 is arranged on the right part upper end of fuselage 1, alcohol spray The lower end of groove 411 is uniformly provided with least three alcohol nozzles 412 from front to back, alcohol go out groove 413 positioned at alcohol nozzle 412 just under Side, alcohol goes out groove 413 is used for the alcohol that recovery section is sprayed from alcohol nozzle 412;
Described hot pin cylinder 414 is horizontally arranged at the bottom of fuselage 1 from left to right, and heating rod 415 is installed by cylinder block At least three heating first 4155 are provided with hot pin cylinder 414, and on heating rod 415, when heating rod 415 is located at swivel plate 22 Hollow out 222 underface of film perforation when, a heating first 4155 is corresponded to immediately below each hollow out film perforation 222, when heating rod 415 is located at During alcohol 412 underface of nozzle, a heating first 4155 is corresponded to immediately below each alcohol nozzle 412;
Described flame groove 416 is arranged on the left part of fuselage 1, and flame groove 416 is built with alcohol, and the side wall peace of flame groove 416 Equipped with electronic lighter, shifting lid cylinder 418 is horizontally installed with the fuselage 1 of the top of flame groove 416 from left to right, moves lid cylinder Flat square door 417 is installed, when door 417 is located at flame 416 surface of groove, door 417 is by flame groove 416 on 418 It is completely enclosed.
During work, when the slide that sputum has been spread in painting is together moved at dyeing apparatus 4 with swivel plate 22, and need to glass When piece is heated, then heater 41 works, under hot pin cylinder 414 drives heating rod 415 to move to alcohol spray groove 411 just Side, the first 4155 sprinkling alcohol of three heating of the alcohol spray groove 411 by alcohol nozzle 412 to heating rod 415, alcohol in sprinkling Heating rod 415 moves to flame groove 416 in the presence of hot pin cylinder 414 again, and now, the door 417 of the upper end of flame groove 416 exists Disengaging flame groove 416 in the presence of lid cylinder 418 is moved, then, electronic lighter sparking is with so that the alcohol in flame groove 416 fires Burn, so as to light the heating rod 415 positioned at the top of flame groove 416;Then door 417 recloses flame groove 416, burning plus Hot pin 415 is moved to below each slide of swivel plate 22 successively under the drive of hot pin cylinder 414, so as to be added to each slide Heat.
Described dropper 42 include dropping liquid cylinder 421, the storage chamber 423, two of a dropping liquid slide plate 422, storage chamber 424, No. three storage chambers 425, minim pipette 426, liquid-dropping valve, dropping liquid nozzle 427 and dropping liquid collection boxes 428, described dropping liquid cylinder 421 are installed Portion on the fuselage 1, dropping liquid slide plate 422 is arranged on dropping liquid cylinder 421,423, No. two storage chambers 424 of a storage chamber and No. three storage chambers 425 are installed in the upper surface of dropping liquid slide plate 422, and 423, No. two storage chambers 424 of a storage chamber are respective with No. three lower ends of storage chamber 425 One row's dropping liquid nozzle 427 is connected with by minim pipette 426, the quantity of each row's dropping liquid nozzle 427 and position with swivel plate 22 on The hollow out film perforation 222 of each row's hollow out group 221 is corresponding, and described liquid-dropping valve is arranged on minim pipette 426, and described dropping liquid is reclaimed Case 428 is installed on the fuselage 1 and is located at the lower right of dropping liquid nozzle 427, and dropping liquid collection box 428 is used to reclaim from the updip of swivel plate 22 The first dye liquor that falls down, decolorising agent and redye liquid.Divide in described 423, No. two storage chambers 424 of a storage chamber and No. three storage chambers 425 Just dye liquor, decolorising agent are not sequentially arranged with and redye liquid.
, it is necessary to first first dye liquor, now, dropper will be added on slide before heater 41 is heated to slide 42 work, dropping liquid cylinder 421 drives dropping liquid slide plate 422 to move forward and backward, and the motion of dropping liquid slide plate 422 drives and a phase of storage chamber 423 Each dropping liquid nozzle 427 of connection sequentially passes through each slide on swivel plate 22 from front to back, corresponding each dropping liquid nozzle 427 according to It is secondary by each slide during, the first dye liquor in a storage chamber 423 flows to dropping liquid in the presence of liquid-dropping valve from minim pipette 426 In nozzle 427, then flowed out on slide from dropping liquid nozzle 427 so that the sputum coating on just dye liquor covering slide, then again will First dye liquor on slide is discharged by the inclination of swivel plate 22, and slide is together turned into flusher 5 with swivel plate 22 Cleaned so that the first dye liquor on slide will be remained in, then again go back at dyeing apparatus 4 clean slide, repeated above-mentioned identical The step of, slide is decolourized and redyed successively with liquid is redyed using the decolorising agent in No. two storage chambers 424 and No. three storage chambers 425 Treatment;Wherein, first dye liquor is being covered into the scala media that the first dye liquor for covering slide is up to rotated to slide flusher 5 Section, it is necessary to heated by heating rod 415 to slide, untill there is steam in the first dye liquor being heated on slide.
Described flusher 5 includes flushing tank 51, flush pipe 52, flushing valve and sewerage recovering box 53, described flushing Pond 51 is arranged on the right part upper end of fuselage 1, and the lower end of flushing tank 51 sets and is communicated with flush pipe 52, the quantity and swivel plate of flush pipe 52 The quantity of hollow out film perforation 222 is equal on 22, and when swivel plate 22 is located at 5 underface of flusher, each hollow out film perforation 222 is just gone up Side is corresponded to and is disposed with a flush pipe 52, and flushing valve is mounted in each flush pipe 52, and described sewerage recovering box 53 is installed On the fuselage 1 and positioned at the lower rear of flushing tank 51.
During work, the slide for covering just dye liquor, decolorising agent and redying liquid turns to flusher 5 from dyeing apparatus 4, rinses Device 5 is worked, and flushing valve is opened, and the clear water in flushing tank 51 is flowed out on slide from flush pipe 52, cleaned with to slide, Wherein, it is rinsed sewage the flowing obliquely into sewerage recovering box 53 by swivel plate 22 got off.
Specifically, as a kind of preferred technical scheme of the invention, flowing flux is additionally provided with described swivel plate 22 225, stop that frame 224 is connected with flowing flux 225 along being extended out at leak-off chute, the described right-hand member of flowing flux 225 relative to The left end of flowing flux 225 is closer to the outside of fuselage 1;Rotation is flowed out by flowing flux 225 from the leak-off chute liquid out for stopping frame 224 Flap.
Specifically, used as a kind of preferred technical scheme of the invention, the left side of the fuselage 1 is provided with and can will put bottle plate 32 Into shelf 31 and the opening of the bamboo stick insertion Tu Tan mechanisms 33 that will apply phlegm;The front side of fuselage 1 is provided with and is available for alcohol to go out The opening of liquid outflow in groove 413 and dropping liquid collection box 428, and the woven hose that alcohol can be added to flame groove 416;Fuselage 1 Rear side is provided with the opening for being available for the outflow of sewage in sewerage recovering box 53, and is put into swivel plate 22 and from swivel plate 22 for slide The access aperture of taking-up;The top of fuselage 1 is provided with can respectively to a storage chamber 425, wine of storage chamber 424, three of storage chamber 423, two The woven hose of liquid is added in essence spray groove 411 and flushed channel;Other positions of fuselage 1 are in sealing state.
The basic principles, principal features and advantages of the present invention have been shown and described above.The technical staff of the industry should Understand, the present invention is not limited to the above embodiments, the description in above-described embodiment and specification is to illustrate of the invention Principle, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, and these change and change Enter all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle. It is fixed.

Claims (9)

1. a kind of Mycobacterium tuberculosis automatic checkout equipment, it is characterised in that:Including fuselage (1), rotary vane device (2), smear phlegm dress Put (3), dyeing apparatus (4) and flusher (5);PLC, described rotary vane device are installed on described fuselage (1) (2) positioned at fuselage (1) middle part, sputum device (3) is smeared positioned at fuselage (1) left part, dyeing apparatus (4) is anterior positioned at fuselage (1), rinses Device (5) is positioned at fuselage (1) right part;Wherein:
Described rotary vane device (2) to rotate and smear sputum device (3), dye so that slide to be sequentially passed through for placing slide Color device (4) and flusher (5);
Described smears sputum device (3) for disposing sputum bottle, and by the phlegm uniform application in sputum bottle on slide;
Described dyeing apparatus (4) to the slide for wiping phlegm for carrying out just contaminating, decolourize and redying;
Described flusher (5) carries out clear water flushing for the slide during to just contaminating, decolourizing and redye.
2. a kind of Mycobacterium tuberculosis automatic checkout equipment according to claim 1, it is characterised in that:Described blade dress Putting (2) includes electronic rotation disk (21), swivel plate (22) and oscillating cylinder (23);
Described swivel plate (22) quantity is four, and four swivel plates (22) are flat cube plate, and four swivel plates (22) It is uniform equally spaced horizontally hinged on electronic rotation disk (21) top;
At least three row's hollow outs group (221) are evenly arranged with each swivel plate (22), be evenly arranged with each row's hollow out group (221) to Few three hollow out film perforations (222), each hollow out film perforation (222) surrounding is provided with square stop frame (224), and slide can just be embedded in resistance In gear frame (224), respectively stop that frame (224) outer end is provided with leak-off chute;
Described oscillating cylinder (23) is evenly arranged in electronic rotation disk (21) surrounding, and oscillating cylinder (23) bottom is hinged on electricity In dynamic rotating disk (21), oscillating cylinder (23) top is hinged on swivel plate (22).
3. a kind of mycobacterium tuberculosis automatic checkout equipment according to claim 1, it is characterised in that:Described smears phlegm dress Put (3) including shelf (31), put a bottle plate (32) and Tu Tan mechanisms (33);
Described shelf (31) quantity is two, and two shelfs (31) are symmetrically vertically installed at fuselage (1) left part, and two are put Thing plate (31) lower end is provided with the flange for extending internally;
A described bottle plate (32) of putting is flat square plate, puts a bottle plate (32) and can be placed on two flanges of shelf (31), is put Hollow cylinder is evenly arranged with bottle plate (32) and puts a bottle storehouse (321), put the quantity and each rotation that a bottle storehouse (321) is put on a bottle plate (32) The quantity of hollow out film perforation (222) is equal on flap (22), puts a bottle storehouse (321) inner side and is surrounded with elastic sheet rubber;
Respectively put a bottle storehouse (321) right-hand member and be provided with square painting phlegm groove (322), when swivel plate (22) is positioned at putting immediately below a bottle plate (32) When, one is correspond to directly over each hollow out film perforation (222) and applies phlegm groove (322);
Described Tu Tan mechanisms (33) include dynamic plate cylinder (331), the dynamic plate (332) of painting phlegm, painting mental disorder cylinder (333) and contiguous block (334), move plate cylinder (331) to be horizontally arranged at from left to right on the fuselage (1) directly over shelf (31), described painting phlegm is moved Plate (332) is horizontally arranged on dynamic plate cylinder (331), and dynamic plate (332) lower surface of painting phlegm is vertically-mounted painting mental disorder cylinder (333), Apply the quantity of mental disorder cylinder (333) and to put quantity that a bottle storehouse (321) is respectively put on a bottle plate (32) equal, and respectively put a bottle storehouse (321) and just go up Side corresponds to one and applies mental disorder cylinder (333);
Each mental disorder cylinder (333) bottom that applies is fixed with contiguous block (334), and contiguous block (334) middle part is provided with Elastic Cylindrical rubber Glue post (335), elastic rubber column (335) middle part is provided with elongated cylindrical inserted link hole.
4. a kind of mycobacterium tuberculosis automatic checkout equipment according to claim 1, it is characterised in that:Described dyeing dress Putting (4) includes heater (41) and dropper (42);
Described heater (41) integral installation is anterior in fuselage (1), and heater (41) including alcohol spray groove (411), Alcohol nozzle (412), alcohol go out groove (413), hot pin cylinder (414), heating rod (415), flame groove (416), door (417) and Move lid cylinder (418);
Alcohol sprays groove (411) installed in fuselage (1) right part upper end, alcohol spray groove (411) lower end be uniformly provided with from front to back to Few three alcohol nozzles (412), alcohol goes out groove (413) immediately below alcohol nozzle (412);
Described hot pin cylinder (414) is horizontally arranged at fuselage (1) bottom from left to right, and heating rod (415) is pacified by cylinder block At least three heating head (4155) are provided with hot pin cylinder (414), and on heating rod (415), when heating rod (415) position A heating head is corresponded to when immediately below the hollow out film perforation (222) of swivel plate (22), immediately below each hollow out film perforation (222) (4155), when heating rod (415) is immediately below alcohol nozzle (412), one is corresponded to immediately below each alcohol nozzle (412) Heating head (4155);
, installed in fuselage (1) left part, flame groove (416) is built with alcohol, and flame groove (416) side for described flame groove (416) Wall is provided with electronic lighter, is horizontally installed with shifting lid cylinder (418) on the fuselage (1) above flame groove (416) from left to right, Move on lid cylinder (418) and flat square door (417) is installed, when door (417) is directly over flame groove (416), gear Lid (417) is completely enclosed by flame groove (416).
5. a kind of Mycobacterium tuberculosis automatic checkout equipment according to claim 4, described dropper (42) includes Dropping liquid cylinder (421), dropping liquid slide plate (422), storage chamber (423), No. two storage chambers (424), No. three storage chambers (425), a minim pipettes (426), liquid-dropping valve, dropping liquid nozzle (427) and dropping liquid collection box (428), described dropping liquid cylinder (421) is installed in fuselage (1) Top, dropping liquid slide plate (422) on dropping liquid cylinder (421), storage chamber (423), No. two storage chambers (424) and No. three storage chambers (425) dropping liquid slide plate (422) upper surface, and storage chamber (423), No. two storage chambers (424) and No. three storage chambers (425) are installed in Lower end is connected with row's dropping liquid nozzle (427) each via minim pipette (426), respectively the quantity of row's dropping liquid nozzle (427) and position The hollow out film perforation (222) put with each row's hollow out group (221) on swivel plate (22) is corresponding, and described liquid-dropping valve is arranged on dropping liquid On pipe (426), described dropping liquid collection box (428) is on fuselage (1) and positioned at dropping liquid nozzle (427) lower right.
6. a kind of mycobacterium tuberculosis automatic checkout equipment according to claim 1, it is characterised in that:Described flushing dress Putting (5) includes flushing tank (51), flush pipe (52), flushing valve and sewerage recovering box (53), and described flushing tank (51) is arranged on Fuselage (1) right part upper end, flushing tank (51) lower end sets and is communicated with flush pipe (52), the quantity and swivel plate of flush pipe (52) (22) quantity of hollow out film perforation (222) is equal on, and when swivel plate (22) is immediately below flusher (5), each hollow out piece Corresponded to directly over hole (222) and be disposed with a flush pipe (52), flushing valve, described dirt are mounted on each flush pipe (52) Water Sproading case (53) is on fuselage (1) and positioned at flushing tank (51) lower rear.
7. a kind of mycobacterium tuberculosis automatic checkout equipment according to claim 2, it is characterised in that:Described swivel plate (22) it is additionally provided with flowing flux (225) on, stops that frame (224) is connected with flowing flux (225) along being extended out at leak-off chute, Described flowing flux (225) right-hand member is relative to flowing flux (225) left end closer to fuselage (1) outside.
8. a kind of mycobacterium tuberculosis automatic checkout equipment according to claim 1, it is characterised in that:The fuselage (1) Left side is provided with to put a bottle plate (32) and enter shelf (31) and will apply bamboo stick insertion Tu Tan mechanism (33) of phlegm and opens Mouthful;Fuselage (1) front side is provided with the opening for being available for alcohol to go out groove (413) and the interior liquid outflow of dropping liquid collection box (428), Yi Jike The woven hose of alcohol is added to flame groove (416);Fuselage (1) rear side is provided with and is available for the interior sewage outflow of sewerage recovering box (53) Opening, and it is put into swivel plate (22) and the access aperture from swivel plate (22) taking-up for slide;Fuselage (1) top is provided with can A storage chamber (423), No. two storage chambers (424), No. three storage chambers (425), alcohol are sprayed and liquid is added in groove (411) and flushed channel respectively The woven hose of body;Other positions of fuselage (1) are in sealing state.
9. a kind of mycobacterium tuberculosis automatic checkout equipment according to claim 4, it is characterised in that:A described storage Just dye liquor, decolorising agent are sequentially arranged with chamber (423), No. two storage chambers (424) and No. three storage chambers (425) respectively and liquid is redyed.
CN201710045486.4A 2017-01-20 2017-01-20 A kind of Mycobacterium tuberculosis automatic checkout equipment Active CN106771288B (en)

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CN111323285A (en) * 2020-03-11 2020-06-23 桂林医学院 Automatic change dyeing decoloration device

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CN112113818A (en) * 2020-10-09 2020-12-22 嘉兴晶铸生物科技有限公司 Constant-temperature full-automatic cell slide-making integrated device and slide-making method

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CN111323285B (en) * 2020-03-11 2022-12-06 桂林医学院 Automatic change dyeing decoloration device

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