CN111518667B - Liquid medium replacing device with low potential pollution - Google Patents
Liquid medium replacing device with low potential pollution Download PDFInfo
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- CN111518667B CN111518667B CN202010363388.7A CN202010363388A CN111518667B CN 111518667 B CN111518667 B CN 111518667B CN 202010363388 A CN202010363388 A CN 202010363388A CN 111518667 B CN111518667 B CN 111518667B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/14—Pressurized fluid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
Abstract
The invention discloses a liquid culture medium replacing device with small potential pollution, which comprises a box body, a replacing box, an incubator and a supercharging mechanism, wherein the replacing box, the incubator and the supercharging mechanism are arranged in the box body from top to bottom, the replacing box is connected with an incubator pipeline, the replacing box is connected with a supercharging mechanism pipeline, the supercharging mechanism enhances the air pressure in the replacing box, the incubator reduces the internal air pressure through gravity, the replacing box and the incubator exchange the culture medium through high pressure and low pressure, the supercharging mechanism accelerates the drying in the replacing box through air flow, the invention is scientific and reasonable, the use is safe and convenient, the incubator contains the culture medium, cells or microorganisms needing to be cultured are cultured in the incubator, and when the culture medium needs to be replaced, the replacing box and the incubator exchange the culture medium through the air pressure action between the high pressure and the low pressure, the method for replacing the culture medium by high pressure and low pressure is simpler and more convenient.
Description
Technical Field
The invention relates to the technical field of liquid culture medium replacement, in particular to a liquid culture medium replacement device with small potential pollution.
Background
Liquid culture medium (liquid culture medium) is a liquid culture medium for microorganisms or animal and plant cells, and the liquid culture medium is used for a period of time, and the properties of the liquid culture medium, such as acidity and alkalinity, are changed due to metabolism of bacteria, and the like, and replacement is required at this time.
This liquid change device realizes the space transfer to new liquid medium through low pressure and high pressure, and the transfer process is gone on in the enclosure space, has realized the change process of zero pollution through low pressure and high pressure to the transfer of liquid medium in the enclosure space, has avoided the pollution risk of liquid medium at the change in-process.
Therefore, there is a need for a liquid medium exchange device with less potential for contamination to solve the above problems.
Disclosure of Invention
The invention aims to provide a liquid culture medium replacing device with small potential pollution so as to solve the problems in the prior art.
In order to achieve the above object, the present invention provides a liquid medium exchange device with less potential contamination, comprising a housing, an exchange box, an incubator, and a pressurizing mechanism, wherein the exchange box, the incubator, and the pressurizing mechanism are provided in the housing from top to bottom, the exchange box is connected to an incubator pipe, the exchange box is connected to a pressurizing mechanism pipe, the pressurizing mechanism increases the air pressure in the exchange box, the incubator reduces the internal air pressure by gravity, the exchange box and the incubator exchange the medium by high pressure and low pressure, and the pressurizing mechanism accelerates the drying of the inside of the exchange box by air flow. The box provides the support for the installation of change case, incubator and booster mechanism, has held the culture medium in the incubator, and the cell or the microorganism that need cultivate are cultivateed in the incubator, when needing to change the culture medium, adds new liquid medium to the change case, and change case and incubator carry out the change of culture medium through the atmospheric pressure effect between high pressure and the low pressure, and the method of carrying out the culture medium through high pressure and low pressure and changing is simpler, convenient.
As a preferred technical scheme, a bearing plate and a transverse plate are arranged in the box body from top to bottom; the replacing box is arranged on the transverse plate, a siphon is arranged on the replacing box, and the other end of the siphon is arranged in the incubator; a liquid discharge pipe is arranged below the incubator, and the other end of the liquid discharge pipe is fixed with a pressurizing mechanism; the supercharging mechanism comprises a warming box and a recycling box, wherein the warming box is arranged below the transverse plate, and the recycling box is arranged in the box body. The loading board is clean water tank, the installation of water pump provides the support, the diaphragm is for changing the case, the installation of incubator provides the support, the space that the change case held for new liquid medium provides the short time, the siphon provides the passageway for changing the culture medium between change case and the incubator, booster mechanism makes the atmospheric pressure reinforcing in the change case, simultaneously for incubator exhaust useless culture medium provides the space of retrieving, the fluid-discharge tube provides the passageway for incubator exhaust useless culture medium, the incubator heats the useless culture medium in the fluid-discharge tube through the heat of generating of friction, carry out high temperature to cell or microorganism in the useless culture medium and kill, the collection box provides the recovery space to useless culture medium.
As a preferred technical scheme, the bearing plate is located above the incubator, the cleaning water tank and the water pump are arranged above the bearing plate, the water inlet end of the water pump is connected with a pipeline of the cleaning water tank, the water outlet end of the water pump is provided with a liquid conveying pipe, the other end of the liquid conveying pipe is of a conical structure, and the liquid conveying pipe is arranged above the replacing box. The clean water tank is filled with water, the clean water tank provides a water source for the clean change case, the water pump provides power for the water entering change case, the transfer line provides a channel for the water entering change case, the transfer line is set to be of a conical structure, the more the water pump is kept away from, the smaller the inner diameter of the transfer line is, the transmission pressure of water in the transfer line can be guaranteed through the setting of the conical structure of the transfer line, and the water in the transfer line can be sprayed into the change case by sufficient water pressure.
As preferred technical scheme, the change case upper end is provided with the feed liquor pipe, is provided with the liquid changing groove on the terminal surface under the change incasement portion, and the terminal surface is for the flute profile terminal surface under the inside of change case, the liquid changing groove is the arc, and the feed liquor end of liquid changing groove is located the minimum of recess terminal surface, and the play liquid end of liquid changing groove is fixed with the siphon, and the minimum of liquid changing groove is provided with clean pipe, the other end of clean pipe runs through the box, and the one end that clean pipe is close to the liquid changing groove is provided with the solenoid valve. The feed liquor pipe provides the passageway for liquid medium gets into to change the case, and the liquid changing groove provides the passageway for the culture medium in changing the case gets into in the siphon, changes the incasement portion down the terminal surface and can make the culture medium in changing the case because the whole flow directions of difference in height change the liquid groove for the recess terminal surface, and the clean pipe provides the passageway for the sewage discharge after the change case is clean, and the solenoid valve carries out the shutoff to clean pipe, when preventing to change the case and having the culture medium, the culture medium is through clean pipe outflow change case.
As a preferred technical scheme, the other end of the liquid discharge pipe is fixed with the recovery box, one end of the liquid discharge pipe, which is close to the incubator, is provided with an electromagnetic valve, the liquid discharge pipe is a spiral pipeline, the liquid discharge pipe is sleeved outside the warming box, a plurality of groups of filter screens are arranged inside the liquid discharge pipe, and the plurality of groups of filter screens are obliquely arranged inside the liquid discharge pipe. The electromagnetic valve plugs the liquid discharge pipe, when the waste culture medium needs to be discharged in the culture box, the electromagnetic valve is opened, the waste culture medium is discharged out of the culture box through the liquid discharge pipe, the heated waste culture medium is stored in the recovery box, the filter screen divides the clustered microorganisms or cells in the culture medium, the microorganisms or the cells are diffused in the heated culture medium, and the microorganisms or the cells are killed through the high-temperature culture medium.
As preferred technical scheme, booster mechanism still includes and rotates the power unit of being connected with the case that heats, power unit includes air machine, rotation case, the air machine sets up on the box, and one side of air machine is provided with the filter screen, the opposite side and the rotation case pipe connection of air machine, it sets up the below at the case that heats to rotate the case, it is provided with the axis of rotation to rotate the incasement, the case that heats is run through to the one end of axis of rotation, the opposite side that rotates the case respectively with collection box, change case pipe connection. The power mechanism provides power for the temperature rise of the temperature increasing box, the air machine provides rotating power for the temperature increase of the temperature increasing box, the rotating box enables air flow to be converted into rotating power, the filter screen filters dust, particles and the like in the air, the rotating shaft obtains the rotating power from the rotating box and rotates in the temperature increasing box and the rotating box, and the air sucked by the air machine enters the recycling box or the replacing box through the rotating box.
According to the preferable technical scheme, the two sides of the rotary box are respectively provided with an air inlet pipe, one group of air inlet pipes are fixed to the air machine, the other group of air inlet pipes are provided with a three-position four-way electromagnetic valve at one end far away from the rotary box, a radiating pipe and a pressurizing pipe are arranged on the three-position four-way electromagnetic valve, the other end of the radiating pipe is arranged in the recycling box, and the other end of the pressurizing pipe is connected with a pipeline of the replacement box. Air inlet tube connection air machine and tribit four-way solenoid valve, tribit four-way solenoid valve divides the route to the exhaust air in the transfer case, the collection box is connected to the cooling tube, after the air that air machine transmitted passes through the cooling tube and gets into in the collection box, accelerate the radiating rate of the useless culture medium of high temperature in the collection box through the air that gets into, the change case is connected to the pressure boost pipe, after the air that air machine transmitted passes through the pressure boost pipe and gets into in the change case, make the atmospheric pressure reinforcing in the change case, make the atmospheric pressure in the change case become the high pressure.
According to the preferred technical scheme, the rotating shaft is rotatably connected with the rotating box, a plurality of groups of magnetic embedding rotating plates and a plurality of groups of rotating plates are sequentially arranged on the rotating shaft from top to bottom, the plurality of groups of magnetic embedding rotating plates are located in the warming box, and the plurality of groups of rotating plates are located in the rotating box. The rotating plate is installed on a rotating shaft in the rotating box, after air transmitted by the air machine enters the rotating box, the rotating plate drives the rotating shaft to rotate under the pushing of air flow, the magnetism embedding rotating plate is installed on the rotating shaft, and when the rotating shaft rotates under the driving of the rotating plate, the rotating shaft drives the magnetism embedding rotating plate to rotate in the temperature increasing box.
According to a preferable technical scheme, the inner end face of the warming box is an embedded magnetic end face, the end faces of the embedded magnetic rotating plate, which are contacted with the inner end face of the warming box, are all embedded magnetic end faces, and the embedded magnetic rotating plate is rotatably connected with the warming box. The inside terminal surface of heating cabinet is for inlaying the magnetism terminal surface, and the terminal surface of inlaying magnetism rotating plate also is for inlaying the magnetism terminal surface, and when inlaying magnetism rotating plate and rotate in the heating cabinet, the heating cabinet utilizes the magnetocaloric effect with inlaying magnetism rotating plate and carries out the frictional heating, makes the box of heating cabinet generate heat to the culture medium in the fluid-discharge tube heats.
As a preferred technical scheme, a plurality of groups of heat dissipation plates are arranged on the outer side of the warming box and are positioned on two sides of the liquid discharge pipe. The heating panel dispels the heat to the high temperature on the case body of the incubator, accelerates the heating to culture medium in the fluid-discharge tube through the setting of heating panel, through diversified heating, accelerates the kill of microorganism or cell in the culture medium.
Compared with the prior art, the invention has the beneficial effects that:
1. the culture medium is contained in the incubator, cells or microorganisms needing to be cultured are cultured in the incubator, when the culture medium needs to be replaced, a new liquid culture medium is added into the replacement box, the replacement box and the incubator carry out replacement of the culture medium through the action of air pressure between high pressure and low pressure, the method for replacing the culture medium through high pressure and low pressure is simpler and more convenient, and the risk that the liquid culture medium is polluted in the transfer process does not exist.
2. After new liquid medium enters the incubator through liquid changing tank and siphon in the change case, water in the water pump extraction clean water tank and through conical transfer line and spray pipe with water injection to change in the case, remaining liquid medium can be washed out, wash on the change case box through spun water in the spray pipe, make remaining liquid medium flow out along with rivers and change the case together, and flow out through clean pipe and change device, avoid remaining liquid culture gene to be detained for a long time in the change case and the phenomenon of growing mould appears, avoid remaining liquid medium to pollute the change case.
3. The moisture in the change case flows out the change case under the effect of the high temperature air current that flows fast to the inside drying process is accelerated under the effect of high temperature air current to the change case, shortens the drying time of change case, cleans and dries through rivers and air current to change incasement portion, avoids changing the case because of remaining the condition that the medium appears mildewing, reduces the potential pollution of liquid medium change in-process.
Drawings
FIG. 1 is a schematic view showing the overall structure of a liquid medium exchange apparatus with less potential contamination according to the present invention;
FIG. 2 is a schematic view of the installation of the overall structural components of a liquid medium exchange device with low potential contamination according to the present invention;
FIG. 3 is a front sectional view showing the overall structure of a liquid medium exchange apparatus with less potential contamination according to the present invention;
FIG. 4 is a schematic view of the area A in FIG. 3 of a liquid medium exchange device with less potential contamination according to the present invention;
FIG. 5 is a schematic view showing the internal structure of a rotary tank of a small-potential contamination liquid medium exchange device according to the present invention;
FIG. 6 is a schematic diagram of the structure of the filter net or the filter net of the liquid culture medium exchange device with less potential pollution.
The reference numbers are as follows: 1. a box body; 2. replacing the box; 3. an incubator; 4. a pressurization mechanism; 1-1, carrying plate; 1-2, a transverse plate; 1-11, cleaning a water tank; 1-12, a water pump; 1-13 parts of infusion tube; 2-1, siphon; 2-2, a liquid changing groove; 2-3, cleaning the tube; 2-4, a liquid inlet pipe; 3-1, a liquid discharge pipe; 3-11 parts of a filter screen; 4-1, a temperature increasing box; 4-2, a recycling bin; 4-3, a power mechanism; 4-11, a heat dissipation plate; 4-31, an air machine; 4-32, a rotating box; 4-33, a filter screen; 4-34, a rotating shaft; 4-35 parts of air inlet pipe; 4-36, three-position four-way electromagnetic valve; 4-37, radiating pipes; 4-38, a pressure increasing pipe; 4-39, embedding a magnetic rotating plate; 4-40, and rotating the plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b): as shown in fig. 1 to 6, a liquid medium exchange device with less potential pollution, the medium exchange device comprises a box body 1, an exchange box 2, an incubator 3, and a pressurization mechanism 4, wherein the exchange box 2, the incubator 3, and the pressurization mechanism 4 are installed in the box body 1 from top to bottom, the exchange box 2 is connected to the incubator 3 through a pipeline, the exchange box 2 is connected to the pressurization mechanism 4 through a pipeline, the pressurization mechanism 4 increases the air pressure in the exchange box 2, the incubator 3 reduces the internal air pressure through gravity, the exchange box 2 and the incubator 3 perform medium exchange through high pressure and low pressure, and the pressurization mechanism 4 accelerates the drying of the inside of the exchange box 2 through air flow.
The replacing device also comprises a control system consisting of a PLC controller, wherein the PLC controller is electrically connected with the water pump 1-12, the three-position four-way electromagnetic valve 4-36, the air machine 4-31 and all the electromagnetic valves through leads, the PLC controller controls the water pump 1-12, the three-position four-way electromagnetic valve 4-36, the air machine 4-31 and the electromagnetic valves, and the air machine 4-31 is a device which is driven by electricity to generate airflow, such as a fan, an exhaust fan and the like.
A bearing plate 1-1 and a transverse plate 1-2 are welded in a box body 1 from top to bottom, a replacing box 2 is fixed on the transverse plate 1-2 through screws, the bearing plate 1-1 is located above an incubator 3, a clean water tank 1-11 and a water pump 1-12 are fixed above the bearing plate 1-1 through screws, the water inlet end of the water pump 1-12 is connected with the clean water tank 1-11 through a pipeline, a liquid conveying pipe 1-13 is fixed at the water outlet end of the water pump 1-12 through screws, the other end of the liquid conveying pipe 1-13 is of a conical structure, the liquid conveying pipe 1-13 is located above the replacing box 2, a water spraying pipe is welded on the end face, in contact with the replacing box 2, of the liquid conveying pipe 1-13, and one end of the water spraying pipe penetrates through.
When the new liquid culture medium in the replacement box 2 completely enters the culture box 3 through the liquid change tank 2-2 and the siphon 2-1, the electromagnetic valve on the siphon 2-1 closes the passage of the siphon 2-1, meanwhile, the electromagnetic valve in the cleaning pipe 2-3 is opened, the pipeline of the cleaning pipe 2-3 is conducted, the water pump 1-12 extracts the water in the cleaning water tank 1-11 and sprays the water into the replacement box 2 through the conical liquid conveying pipe 1-13 and the water spraying pipe, the residual liquid culture medium on the box body of the replacement box 2 can be washed and cleaned through the water sprayed from the water spraying pipe, the residual liquid culture medium flows out of the replacement box 2 along with the water flow, and flows out of the replacement device through the cleaning pipe 2-3, so that the phenomenon that the residual liquid culture genes are retained in the replacement box 2 for a long time and mildewing is avoided, avoiding the residual liquid culture medium from polluting the replacement box 2.
The siphon 2-1 is welded on the replacement box 2, the other end of the siphon 2-1 is welded in the incubator 3, the liquid outlet end of the siphon 2-1 is lower than the liquid inlet end of the replacement box 2, the electromagnetic valve is installed in the middle of the siphon 2-1 and controls the conduction or the closing of the pipeline of the siphon 2-1, when new culture medium is added into the replacement box 2 and the incubator 3 discharges waste culture medium, the electromagnetic valve closes the pipeline of the siphon 2-1, so that the exchange of gas between the replacement box 2 and the incubator 3 cannot be carried out, and when new liquid culture medium in the replacement box 2 needs to enter the incubator 3, the electromagnetic valve conducts the pipeline of the siphon 2-1, so that the new liquid culture medium enters the incubator 3 through the siphon 2-1.
The upper end face of the replacing box 2 is welded with a liquid inlet pipe 2-4, an electromagnetic valve is installed in the liquid inlet pipe 2-4, the electromagnetic valve conducts or closes a pipeline of the liquid inlet pipe 2-4, a liquid replacing groove 2-2 is machined in the lower end face of the inside of the replacing box 2, the lower end face of the inside of the replacing box 2 is a groove-shaped end face, the liquid replacing groove 2-2 is arc-shaped, the liquid inlet end of the liquid replacing groove 2-2 is located at the lowest point of the groove end face, the liquid outlet end of the liquid replacing groove 2-2 is welded with a siphon pipe 2-1, a cleaning pipe 2-3 is welded at the lowest end of the liquid replacing groove 2-2, the other end of the cleaning pipe 2-3 penetrates through the box body 1, and the electromagnetic valve is installed at one end, close to the liquid.
A liquid discharge pipe 3-1 is welded below the incubator 3, an electromagnetic valve is installed at one end, close to the incubator 3, of the liquid discharge pipe 3-1, the middle portion of the liquid discharge pipe 3-1 is a spiral pipeline, the spiral portion of the liquid discharge pipe 3-1 is sleeved on the outer side of the warming box 4-1, the other end of the liquid discharge pipe 3-1 is welded with the recovery box 4-2, a plurality of groups of filter screens 3-11 are installed inside the liquid discharge pipe 3-1, the filter screens 3-11 are installed inside the liquid discharge pipe 3-1 in an inclined mode, and the filter screens 3-11 are used for cutting microorganisms or cells which are clustered in a culture medium.
Further optimization, a liquid level detector can be arranged in the incubator 3 and used for monitoring the liquid level of the incubator 3, when the liquid level of the incubator 3 drops to a set liquid level height, the liquid level detector transmits a signal to a PLC (programmable logic controller), and the PLC closes an electromagnetic valve in a liquid discharge pipe 3-1 to stop the liquid culture medium in the incubator 3 from flowing out; or, the incubator 3 and the box body 1 are made of transparent materials, when the incubator 3 discharges the culture medium, an operator can see the discharge progress of the culture medium at the outer side of the box body 1 and manually transmit a stop signal to the PLC controller, so that the culture medium in the incubator 3 is not discharged.
The supercharging mechanism 4 comprises a warming box 4-1 and a recovery box 4-2, the warming box 4-1 is fixed below a transverse plate 1-2 through screws, a plurality of groups of heat dissipation plates 4-11 are welded on the outer side of the warming box 4-1, the plurality of groups of heat dissipation plates 4-11 are combined in pairs, the heat dissipation plates 4-11 combined in pairs are located on two sides of a liquid discharge pipe 3-1, the heat dissipation plates 4-11 clamp and fix the liquid discharge pipe 3-1, heat on the warming box 4-1 is transferred to the liquid discharge pipe 3-1 when the warming box 4-1 starts to heat, the recovery box 4-2 is fixed in a box body 1 through screws, and a high-temperature pipe is welded on the upper end face of the recovery box 4-2.
Further, the bottom of the recycling box 4-2 can be provided with a waste liquid discharging pipeline, and the waste liquid of the recycling box 4-2 is discharged out of the recycling box 4-2 through the pipeline.
The supercharging mechanism 4 also comprises a power mechanism 4-3 rotationally connected with the warming box 4-1, the power mechanism 4-3 comprises an air machine 4-31 and a rotating box 4-32, the air machine 4-31 is fixed on the box body 1 through screws, one side of the air machine 4-31 is fixed with a filter screen 4-33 through screws, the other side of the air machine 4-31 is fixed with a gas collecting pipe through screws, the other end of the gas collecting pipe is connected with a pipeline of the rotating box 4-32, the rotating box 4-32 is fixed below the warming box 4-1 through screws, two sides of the rotating box 4-32 are respectively welded with gas inlet pipes 4-35, one group of gas inlet pipes 4-35 are welded with the gas collecting pipe on the air machine 4-31, one end of the other group of gas inlet pipes 4-35 far away from the rotating box 4-32 is fixed with a three-position four-way electromagnetic valve, the rotating shaft 4-34 is rotatably arranged in the rotating box 4-32, and one end of the rotating shaft 4-34 penetrates through the warming box 4-1.
The three-position four-way electromagnetic valve 4-36 is provided with a radiating pipe 4-37 and a pressure increasing pipe 4-38, the other end of the radiating pipe 4-37 is arranged in the recycling tank 4-2 and is positioned on the inner lower end face of the recycling tank 4-2, an air outlet is processed on the radiating pipe 4-37, the other end of the pressure increasing pipe 4-38 is connected with the replacing tank 2 through a pipeline, a three-way pipe is arranged on the pressure increasing pipe 4-38 and divides the pressure increasing pipe 4-38 into two sections, the rest end of the three-way pipe is connected with the high temperature pipe, two air inlet ends of the three-way pipe are respectively connected with the pressure increasing pipe 4-38 and the high temperature pipe, electromagnetic valves are arranged in the two air inlet ends, the two air inlet ends are controlled by the electromagnetic valves, and in an initial state, the two electromagnetic.
The rotating shaft 4-34 is rotatably connected with the rotating box 4-32, a plurality of groups of magnetic embedded rotating plates 4-39 and a plurality of groups of rotating plates 4-40 are sequentially arranged on the rotating shaft 4-34 from top to bottom, and the plurality of groups of rotating plates 4-40 are positioned in the rotating box 4-32.
The magnetic embedded rotating plates 4-39 are positioned in the temperature increasing box 4-1, the inner end face of the temperature increasing box 4-1 is a magnetic embedded end face, the end faces of the magnetic embedded rotating plates 4-39, which are contacted with the inner end face of the temperature increasing box 4-1, are magnetic embedded end faces, and the magnetic embedded rotating plates 4-39 are rotatably connected with the temperature increasing box 4-1.
The working principle of the invention is as follows:
when the liquid culture medium in the incubator 3 needs to be replaced, the PLC controls the electromagnetic conduction in the liquid inlet pipe 2-4, an operator adds a new liquid culture medium into the replacement box 2 through the liquid inlet pipe 2-4, after the culture medium is added, the PLC controls the electromagnetic valve to close the channel of the liquid inlet pipe 2-4, meanwhile, the PLC controls the air machine 4-31 to start working, controls the three-position four-way electromagnetic valve 4-36 to be communicated with the pressure pipe 4-38 and the three-way pipe to be communicated with the whole pressure pipe 4-38, air pumped by the air machine 4-31 enters the rotating box 4-32 through the air collecting pipe and the air inlet pipe 4-35, the rotating plate 4-40 rotates together with the rotating shaft 4-34 in the rotating box 4-32 under the pushing of air flow, the rotating shaft 4-34 drives the magnetic rotating plate 4-39 to rotate in the heating box 4-1, the temperature increasing box 4-1 and the magnetic embedded rotating plate 4-39 generate heat through friction by using a magnetic thermal effect, so that a box body of the temperature increasing box 4-1 generates heat, the temperature increasing box 4-1 heats air entering the liquid discharge pipe 3-1 through the heat dissipation plate 4-11, air in the rotating box 4-32 enters from the gas collection pipe, the pressure increasing pipe 4-38 discharges the air, the air extracted by the air machine 4-31 enters the replacement box 2 through the pressure increasing pipe 4-38, and the air pressure in the replacement box 2 is continuously increased through the continuously entering air, so that the air pressure in the replacement box 2 is changed into high pressure.
After the PLC controller controls the air machine 4-31 to work for a period of time (the working time is set by an operator), the PLC controller switches on an electromagnetic valve in the liquid discharge pipe 3-1, the waste culture medium in the culture box 3 flows out of the culture box 3 under the action of self gravity and is discharged into the recovery box 4-2 through the heated liquid discharge pipe 3-1, when the waste culture medium passes through the spiral liquid discharge pipe 3-1, microorganisms or cells in the waste culture medium are killed under the action of high temperature, and because the siphon pipe 2-1 is in a closed state under the action of the electromagnetic valve, the culture box 3 and the replacement box 2 cannot exchange gas and culture medium, so that the air pressure in the culture box 3 is continuously reduced under the condition that the culture medium is continuously reduced, and the air pressure of the culture box 3 is low pressure.
When the culture medium in the incubator 3 is discharged to a certain extent, the PLC closes the electromagnetic valve in the discharge pipe 3-1 and makes the electromagnetic valve in the siphon 2-1 large, new culture medium in the incubator 2 enters the incubator 3 through the siphon 2-1 under the action of high pressure in the incubator 2 and low pressure in the incubator 3, and after all the culture medium enters the incubator 3, gas in the incubator 2 enters the incubator 3 through the siphon 2-1, so that the air pressure in the incubator 3 is in a normal pressure state.
When the new liquid culture medium in the replacement box 2 completely enters the culture box 3 through the liquid change tank 2-2 and the siphon 2-1, the electromagnetic valve on the siphon 2-1 closes the passage of the siphon 2-1, meanwhile, the electromagnetic valve in the cleaning pipe 2-3 is opened, the pipeline of the cleaning pipe 2-3 is conducted, the water pump 1-12 extracts the water in the cleaning water tank 1-11 and sprays the water into the replacement box 2 through the conical liquid conveying pipe 1-13 and the water spraying pipe, the residual liquid culture medium on the box body of the replacement box 2 can be washed and cleaned through the water sprayed from the water spraying pipe, the residual liquid culture medium flows out of the replacement box 2 along with the water flow, and flows out of the replacement device through the cleaning pipe 2-3, so that the phenomenon that the residual liquid culture genes are retained in the replacement box 2 for a long time and mildewing is avoided, avoiding the residual liquid culture medium from polluting the replacement box 2.
And the PLC controller controls the three-way pipe to be communicated with the high-temperature pipe and the pressure increasing pipe 4-38, controls the three-position four-way electromagnetic valve 4-36 to be communicated with the heat radiating pipe 4-37 and the air inlet pipe 4-35, air pumped by the air machine 4-31 enters the recovery box 4-2 through the air inlet pipe 4-35 and the heat radiating pipe 4-37, the high-temperature culture medium in the recovery box 4-2 generates bubbles and accelerates the heat radiation under the injection of air, the heat radiated by the culture medium is taken out of the recovery box 4-2 under the action of the air flow and enters the replacement box 2 through the high-temperature pipe and the pressure increasing pipe 4-38, the moisture in the replacement box 2 flows out of the replacement box 2 under the action of the fast flowing high-temperature air flow, the drying process of the interior of the replacement box 2 is accelerated under the action of the high-temperature air flow, the interior of the replacement box 2 is cleaned and dried through water flow and air flow, so that the mildew condition caused by residual culture medium in the replacement box 2 is avoided, and the potential pollution in the liquid culture medium replacement process is reduced.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (8)
1. A liquid culture medium replacement device with low potential pollution is characterized in that: the culture medium replacing device comprises a box body (1), a replacing box (2), an incubator (3) and a supercharging mechanism (4), wherein the replacing box (2), the incubator (3) and the supercharging mechanism (4) are arranged in the box body (1) from top to bottom, the replacing box (2) is connected with the incubator (3) through a pipeline, the replacing box (2) is connected with the supercharging mechanism (4) through a pipeline, the supercharging mechanism (4) enables the air pressure in the replacing box (2) to be enhanced, the incubator (3) enables the internal air pressure to be reduced through gravity, the replacing box (2) and the incubator (3) carry out culture medium replacement through high pressure and low pressure, and the supercharging mechanism (4) accelerates the drying of the interior of the replacing box (2) through air flow;
a bearing plate (1-1) and a transverse plate (1-2) are arranged in the box body (1) from top to bottom; the replacing box (2) is arranged on the transverse plate (1-2), a siphon (2-1) is arranged on the replacing box (2), and the other end of the siphon (2-1) is arranged in the incubator (3); a liquid discharge pipe (3-1) is arranged below the incubator (3), and the other end of the liquid discharge pipe (3-1) is fixed with a pressurizing mechanism (4); the supercharging mechanism (4) comprises a warming box (4-1) and a recovery box (4-2), wherein the warming box (4-1) is arranged below the transverse plate (1-2), and the recovery box (4-2) is arranged in the box body (1);
the supercharging mechanism (4) further comprises a power mechanism (4-3) rotatably connected with the warming box (4-1), the power mechanism (4-3) comprises an air machine (4-31) and a rotating box (4-32), the air machine (4-31) is arranged on the box body (1), a filter screen (4-33) is arranged on one side of the air machine (4-31), the other side of the air machine (4-31) is connected with the rotating box (4-32) through a pipeline, the rotating box (4-32) is arranged below the warming box (4-1), a rotating shaft (4-34) is arranged in the rotating box (4-32), one end of the rotating shaft (4-34) penetrates through the warming box (4-1), and the other side of the rotating box (4-32) is respectively connected with a recycling box (4-2), The replacement box (2) is connected by pipelines.
2. A liquid medium exchange device with low potential contamination according to claim 1, wherein: the water-saving culture box is characterized in that the bearing plate (1-1) is located above the culture box (3), a clean water tank (1-11) and a water pump (1-12) are arranged above the bearing plate (1-1), the water inlet end of the water pump (1-12) is connected with the clean water tank (1-11) through a pipeline, a liquid conveying pipe (1-13) is arranged at the water outlet end of the water pump (1-12), the other end of the liquid conveying pipe (1-13) is of a conical structure, and the liquid conveying pipe (1-13) is arranged above the replacement box (2).
3. A liquid medium exchange device with low potential contamination according to claim 1, wherein: the upper end of the replacement box (2) is provided with a liquid inlet pipe (2-4), the lower end face inside the replacement box (2) is provided with a liquid replacement groove (2-2), the lower end face inside the replacement box (2) is a groove-shaped end face, the liquid replacement groove (2-2) is arc-shaped, the liquid inlet end of the liquid replacement groove (2-2) is located at the lowest point of the groove end face, the liquid outlet end of the liquid replacement groove (2-2) is fixed with a siphon (2-1), the lowest end of the liquid replacement groove (2-2) is provided with a cleaning pipe (2-3), the other end of the cleaning pipe (2-3) penetrates through the box body (1), and one end, close to the liquid replacement groove (2-2), of the cleaning pipe (2-3) is provided with.
4. A liquid medium exchange device with low potential contamination according to claim 1, wherein: the other end of the liquid discharge pipe (3-1) is fixed with the recovery box (4-2), an electromagnetic valve is arranged at one end, close to the incubator (3), of the liquid discharge pipe (3-1), the liquid discharge pipe (3-1) is a spiral pipeline, the liquid discharge pipe (3-1) is sleeved on the outer side of the warming box (4-1), a plurality of groups of filter screens (3-11) are arranged inside the liquid discharge pipe (3-1), and the plurality of groups of filter screens (3-11) are obliquely arranged inside the liquid discharge pipe (3-1).
5. A liquid medium exchange device with low potential contamination according to claim 1, wherein: the air inlet pipe (4-35) is arranged on each of two sides of the rotary box (4-32), one group of air inlet pipes (4-35) is fixed with the air machine (4-31), one end, far away from the rotary box (4-32), of the other group of air inlet pipes (4-35) is provided with a three-position four-way electromagnetic valve (4-36), the three-position four-way electromagnetic valve (4-36) is provided with a radiating pipe (4-37) and a pressurizing pipe (4-38), the other end of the radiating pipe (4-37) is arranged in the recovery box (4-2), and the other end of the pressurizing pipe (4-38) is connected with the replacement box (2) through a pipeline.
6. A liquid culture medium exchange device with low potential pollution according to claim 5, wherein: the rotating shaft (4-34) is rotatably connected with the rotating box (4-32), a plurality of groups of magnetic embedding rotating plates (4-39) and a plurality of groups of rotating plates (4-40) are sequentially arranged on the rotating shaft (4-34) from top to bottom, the plurality of groups of magnetic embedding rotating plates (4-39) are positioned in the warming box (4-1), and the plurality of groups of rotating plates (4-40) are positioned in the rotating box (4-32).
7. A liquid culture medium exchange device with low potential pollution according to claim 6, wherein: the inner end face of the warming box (4-1) is an embedded magnetic end face, the end faces of the embedded magnetic rotating plates (4-39) contacting with the inner end face of the warming box (4-1) are all embedded magnetic end faces, and the embedded magnetic rotating plates (4-39) are rotatably connected with the warming box (4-1).
8. A liquid culture medium exchange device with low potential pollution according to claim 4, wherein: the outer side of the warming box (4-1) is provided with a plurality of groups of heat dissipation plates (4-11), and the plurality of groups of heat dissipation plates (4-11) are positioned on two sides of the liquid discharge pipe (3-1).
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| CN202010363388.7A CN111518667B (en) | 2020-04-30 | 2020-04-30 | Liquid medium replacing device with low potential pollution |
| CN202110385726.1A CN112899164A (en) | 2020-04-30 | 2020-04-30 | Liquid culture medium replacing device with low potential pollution |
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| CN111518667A (en) | 2020-08-11 |
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