CN108456627A - A kind of cell culture medium automatic fermenter with high dispersive sterilizing - Google Patents
A kind of cell culture medium automatic fermenter with high dispersive sterilizing Download PDFInfo
- Publication number
- CN108456627A CN108456627A CN201810630983.5A CN201810630983A CN108456627A CN 108456627 A CN108456627 A CN 108456627A CN 201810630983 A CN201810630983 A CN 201810630983A CN 108456627 A CN108456627 A CN 108456627A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- pedestal
- cell culture
- liquid
- pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Abstract
The invention discloses a kind of cell culture medium automatic fermenters with high dispersive sterilizing,Including pedestal,The upper surface left end of pedestal is equipped with liquid storing barrel,Positioning column is equipped in the middle part of the upper surface of liquid storing barrel,The upper surface of positioning column is equipped with steam generating box,The medial surface lower end of steam generating box is equipped with electric heating sheets,The upper surface of steam generating box is equipped with liquid pump,The inlet of liquid pump is connected by conduit with the lateral surface lower end of liquid storing barrel,This has the cell culture medium automatic fermenter of high dispersive sterilizing,It is compact-sized,It is easy to operate,It occupies little space when use,Can high-temperature sterilization processing quickly very easily be carried out to culture medium,The setting of storage tank facilitates the storage of tool,Stirring blade can uniformly stir culture medium,Be conducive to culture medium to come into full contact with high-temperature steam,The dust contained in air can be filtered by filtering dust net.
Description
Technical field
The present invention relates to cell culture apparatus technical field, specially a kind of cell culture medium with high dispersive sterilizing is complete
Fermentation.
Background technology
The sterilizing in batches of cell culture medium is exactly that prepared culture medium is placed in fermentation tank or other devices, is passed through steaming
Culture medium and device therefor are carried out the process of heat sterilization by vapour together, and also commonly referred to as tank sterilizes.Nowadays going out in the market
Bacterium fermentation tank mostly cleans inconvenience, and at the same time existing disinfection fermentation tank cannot carry out clearly rapid draing after the completion of cleaning, together
Shi Xianyou disinfection fermentation tanks when sterilizing to culture medium often due to culture medium cannot fully with high-temperature steam contact, from
And cause medium sterilization not thorough enough.
Invention content
The technical problem to be solved by the present invention is to overcome the existing defects, provides a kind of cell training with high dispersive sterilizing
Base automatic fermenter is supported, quickly fermentation tank can be cleaned, be dried, brought just to the cleaning use of fermentation tank
Profit, and uniform stirring sterilization treatment can be carried out to cell culture medium, the service efficiency of the present invention is further improved, it can
Effectively to solve the problems in background technology.
To achieve the above object, the present invention provides the following technical solutions：A kind of cell culture medium with high dispersive sterilizing
The upper surface left end of automatic fermenter, including pedestal, the pedestal is equipped with liquid storing barrel, and it is fixed to be equipped in the middle part of the upper surface of liquid storing barrel
The upper surface of position column, positioning column is equipped with steam generating box, and the medial surface lower end of steam generating box is equipped with electric heating sheets, steam generation
The upper surface of case is equipped with liquid pump, and the inlet of liquid pump is connected by conduit with the lateral surface lower end of liquid storing barrel, the liquid outlet of liquid pump
It is connected with the upper surface of steam generating box by the 4th diversion pipe, the lateral surface of the 4th diversion pipe is close to steam generating box upper surface
One end be equipped with the 4th water conservancy diversion tube valve, the surface of pedestal is equipped with fermentation tank, isocon is equipped on the left of the upper surface of fermentation tank,
The lower face of isocon extends to the medial surface bottom of fermentation tank by runner pipe, is led by third in the middle part of the lateral surface of isocon
It is connected in the middle part of flow tube and the lateral surface of the 4th diversion pipe, third diversion pipe is equipped with third water conservancy diversion tube valve, steam generating box
Right side is equipped with aspiration pump, and the air inlet of aspiration pump is connected by conduit with the right side upper end of steam generating box, aspiration pump
Gas outlet is connected by the second diversion pipe with the lateral surface lower end of isocon, and the second diversion pipe is equipped with the second water conservancy diversion tube valve,
Stirring motor is equipped in the middle part of the upper surface of fermentation tank, the output shaft of stirring motor is equipped with agitating shaft, and the lateral surface of agitating shaft is equipped with
The medial surface upper end of stirring blade, fermentation tank is equipped with temperature sensor, and supporting rack, support are equipped on rear side of the upper surface middle part of pedestal
The upper surface of frame is equipped with Rose Box, and the upper surface of Rose Box is equipped with blower pump, and the air inlet of blower pump passes through conduit and Rose Box
Leading flank be connected, the gas outlet of blower pump is connected by the 5th diversion pipe with the lateral surface upper end of isocon, the 5th diversion pipe
It is equipped with the 5th water conservancy diversion tube valve, is equipped with drying box, microcontroller and air pump on the left of the upper surface of pedestal, drying box is internally provided with
The leading flank of drier, drying box is equipped with seal cover board, and the left side of drying box is equipped with the upper end of the first diversion pipe and fermentation tank
Face is connected, and the first diversion pipe is equipped with the first water conservancy diversion tube valve, the right side phase that the air inlet of air pump passes through conduit and drying box
Even, the input terminal of microcontroller is electrically connected with the output end of external power supply, and the output end of microcontroller is electric with aspiration pump, stirring respectively
The input terminal electrical connection of machine, aspiration pump, liquid pump, electric heating sheets and blower pump, temperature sensor and microcontroller are bi-directionally connected.
As a preferred technical solution of the present invention, the pedestal is socketed with by being disposed thereon the location hole on surface
Screw rod, and the quantity of screw rod is four, four screw rods are evenly distributed on the upper surface surrounding of pedestal, and the lower face of screw rod, which is equipped with, prevents
Sliding block.
As a preferred technical solution of the present invention, the threaded one end of the lateral surface of the screw rod close to base upper surface
It is connected with the first nut, the lateral surface of screw rod is connected with the second nut close to the threaded one end of base lower surface.
As a preferred technical solution of the present invention, the lateral surface front end of the liquid storing barrel be equipped with observation window of liquid level mouth and
Liquid-feeding tube, liquid-feeding tube are equipped with liquid feeding tube valve.
As a preferred technical solution of the present invention, locating ring, locating ring are equipped in the middle part of the lateral surface of the fermentation tank
Lower surface be connected with the upper surface of pedestal by supporting rod.
As a preferred technical solution of the present invention, the quantity of the supporting rod is three, and three supporting rods uniformly divide
Lower surface surrounding of the cloth in locating ring.
As a preferred technical solution of the present invention, charge pipe is equipped on rear side of the upper surface of the fermentation tank, and feed
The quantity of pipe is three, and charging tube valve is equipped on three charge pipes.
As a preferred technical solution of the present invention, it is equipped with probe tube on front side of the lateral surface lower end of the fermentation tank, takes
Sample pipe is equipped with sampling tube valve, and the lower face of fermentation tank is equipped with drainage conduit, and drainage conduit is equipped with discharge tube valve.
As a preferred technical solution of the present invention, the medial surface of the Rose Box is equipped with filtering dust net, Rose Box
Left side be equipped with maintenance window, the trailing flank of Rose Box is equipped with air-inlet grille.
As a preferred technical solution of the present invention, the upper surface left end of the pedestal is equipped with storage tank, and storage tank is logical
It crosses the hinge being arranged in its leading flank right end and is rotatably connected to front shroud, left end is equipped with handle in the middle part of the leading flank of front shroud.
Compared with prior art, the beneficial effects of the invention are as follows：This has the cell culture medium of high dispersive sterilizing full-automatic
Fermentation tank, compact-sized, easy to operate, when use, occupies little space, and very easily quickly can carry out high temperature to culture medium and go out
Bacterium is handled, and the setting of storage tank facilitates the storage of tool, and stirring blade can uniformly stir culture medium, be conducive to
Culture medium comes into full contact with high-temperature steam, can be filtered to the dust contained in air by filtering dust net.
Description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is front view of the present invention；
Fig. 3 is left view of the present invention.
In figure：1 pedestal, 2 non-slipping blocks, 3 screw rods, 4 observation window of liquid level mouths, 5 supporting rods, 6 fermentation tanks, 7 probe tubes, 8 sealings
Cover board, 9 microcontrollers, 10 aspiration pumps, 11 front shrouds, 12 storage tanks, 13 drying boxes, 14 first diversion pipes, 15 stirring motors, 16 add
Expects pipe, 17 locating rings, 18 steam generating box, 19 liquid storing barrels, 20 agitating shafts, 21 temperature sensors, 22 isocons, 23 second water conservancy diversion
Pipe, 24 aspiration pumps, 25 third diversion pipes, 26 the 4th diversion pipes, 27 liquid pumps, 28 electric heating sheets, 29 drainage conduits, 30 stirring blades,
31 the 5th diversion pipes, 32 blower pumps, 33 filtering dust nets, 34 Rose Boxes, 35 supporting racks.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
- 3 are please referred to Fig.1, the present invention provides a kind of technical solution：A kind of cell culture medium with high dispersive sterilizing is complete certainly
Dynamic fermentation tank, including pedestal 1, pedestal 1 is socketed with screw rod 3 by being disposed thereon the location hole on surface, and the quantity of screw rod 3 is
Four, four screw rods 3 are evenly distributed on the upper surface surrounding of pedestal 1, and the lower face of screw rod 3 is equipped with non-slipping block 2, screw rod 3 it is outer
Threaded one end of the side close to 1 upper surface of pedestal is connected with the first nut, and the lateral surface of screw rod 3 is close to the one of 1 lower surface of pedestal
End is threaded with the second nut, and the second nut combines the setting of the first nut that non-slipping block 2 is very easily risen
The upper surface left end of falling tone section, pedestal 1 is equipped with liquid storing barrel 19, and the lateral surface front end of liquid storing barrel 19 is equipped with 4 He of observation window of liquid level mouth
Liquid-feeding tube, liquid-feeding tube are equipped with liquid feeding tube valve, are equipped with positioning column in the middle part of the upper surface of liquid storing barrel 19, the upper surface of positioning column is set
There are steam generating box 18, the medial surface lower end of steam generating box 18 to be equipped with electric heating sheets 28, electric heating sheets 28 facilitate high temperature steaming
The generation of vapour, the upper surface of steam generating box 18 are equipped with liquid pump 27, and the inlet of liquid pump 27 is outer by conduit and liquid storing barrel 19
Side lower end is connected, and the liquid outlet of liquid pump 27 is connected by the 4th diversion pipe 26 with the upper surface of steam generating box 18, and the 4th leads
The lateral surface of flow tube 26 is equipped with the 4th water conservancy diversion tube valve close to one end of 18 upper surface of steam generating box, and the surface of pedestal 1 is set
There is fermentation tank 6, charge pipe 16 is equipped on rear side of the upper surface of fermentation tank 6, and the quantity of charge pipe 16 is three, three charge pipes 16
On be equipped with charging tube valve, the setting of charge pipe 16 facilitates the charging of raw material, is equipped on front side of the lateral surface lower end of fermentation tank 6
Probe tube 7, probe tube 7 are equipped with sampling tube valve, and the lower face of fermentation tank 6 is equipped with drainage conduit 29, and drainage conduit 29 is equipped with row
The lateral surface middle part of expects pipe valve, fermentation tank 6 is equipped with locating ring 17, and the lower surface of locating ring 17 passes through supporting rod 5 and pedestal 1
Upper surface is connected, and the quantity of supporting rod 5 is three, and three supporting rods 5 are evenly distributed on the lower surface surrounding of locating ring 17, fermentation
Isocon 22 is equipped on the left of the upper surface of tank 6, the lower face of isocon 22 extends to the medial surface bottom of fermentation tank 6 by runner pipe
Portion, by being connected in the middle part of the lateral surface of third diversion pipe 25 and the 4th diversion pipe 26, third is led at the lateral surface middle part of isocon 22
Flow tube 25 is equipped with third water conservancy diversion tube valve, and the setting of third diversion pipe 25 facilitates the cleaning of fermentation tank 6, steam generating box 18
Right side be equipped with aspiration pump 24, the air inlet of aspiration pump 24 is connected by conduit with the right side upper end of steam generating box 18,
The gas outlet of aspiration pump 24 is connected by the second diversion pipe 23 with the lateral surface lower end of isocon 22, and the second diversion pipe 23 is equipped with
The upper surface middle part of second water conservancy diversion tube valve, fermentation tank 6 is equipped with stirring motor 15, and the output shaft of stirring motor 15 is equipped with agitating shaft
20, the lateral surface of agitating shaft 20 is equipped with stirring blade 30, and stirring blade 30 can uniformly stir culture medium, be conducive to
Culture medium comes into full contact with high-temperature steam, and the medial surface upper end of fermentation tank 6 is equipped with temperature sensor 21, in the upper surface of pedestal 1
Supporting rack 35 is equipped on rear side of portion, the upper surface of supporting rack 35 is equipped with Rose Box 34, and the upper surface of Rose Box 34 is equipped with blower pump 32,
The air inlet of blower pump 32 is connected by conduit with the leading flank of Rose Box 34, and the gas outlet of blower pump 32 passes through the 5th diversion pipe
31 are connected with the lateral surface upper end of isocon 22, and the 5th diversion pipe 31 is equipped with the 5th water conservancy diversion tube valve, the inside of Rose Box 34
Face is equipped with filtering dust net 33, can be filtered to the dust contained in air by filtering dust net 33, Rose Box 34
Left side is equipped with maintenance window, and the trailing flank of Rose Box 34 is equipped with air-inlet grille, can will be in fermentation tank 6 by blower pump 32
The upper surface left side of portion's Quick-air-drying, pedestal 1 is equipped with drying box 13, microcontroller 9 and air pump 10, and being internally provided with for drying box 13 is dry
The leading flank of drying prescription, drying box 13 is equipped with seal cover board 8, and the left side of drying box 13 is equipped with the first diversion pipe 14 and fermentation tank 6
Upper surface be connected, the first diversion pipe 14 is equipped with the first water conservancy diversion tube valve, and the air inlet of air pump 10 passes through conduit and drying box
13 right side is connected, and the upper surface left end of pedestal 1 is equipped with storage tank 12, and storage tank 12 is by being arranged in its leading flank right end
Hinge is rotatably connected to front shroud 11, and left end is equipped with handle in the middle part of the leading flank of front shroud 11, and the setting of storage tank 12 facilitates
The storage of tool, the input terminal of microcontroller 9 are electrically connected with the output end of external power supply, the output end of microcontroller 9 respectively with air pump
10, the input terminal electrical connection of stirring motor 15, aspiration pump 24, liquid pump 27, electric heating sheets 28 and blower pump 32, temperature sensor 21
It is bi-directionally connected with microcontroller 9, microcontroller 9 controls air pump 10, stirring motor 15, aspiration pump 24, liquid pump 27, electric heating sheets 28 and blows
Air pump 32 is all made of method commonly used in the prior art, and microcontroller 9 is using in Intel Company's MCS-51 series monolithics
8051, this has cell culture medium automatic fermenter of high dispersive sterilizing, compact-sized, easy to operate, occupies when use empty
Between it is small, can very easily quickly to culture medium carry out high-temperature sterilization processing.
When in use：External power supply is connected, the 4th water conservancy diversion tube valve is closed, third water conservancy diversion tube valve is opened, by microcontroller 9
It controls liquid pump 27 to work, be pumped to the liquid in liquid storing barrel 19 in fermentation tank 6 by liquid pump 27, while being passed through by stirring motor 15
Agitating shaft 20 drives the rotation of stirring blade 30 to clean fermentation tank 6, after the completion of cleaning, opens discharge tube valve by fermentation tank
Liquid discharge in 6, while hot outside being pumped in fermentation tank 6 by blower pump 32 and it is dried processing, it is dry
After the completion of processing, discharge tube valve is closed, culture medium, antifoaming agent and lye are filled into fermentation tank 6 by charge pipe 16, together
When open the 4th water conservancy diversion tube valve, close third water conservancy diversion tube valve, the liquid in liquid storing barrel 19 is pumped into steam by liquid pump 27
Occur in case 18, carrying out heating to liquid by electrical heating 28 makes it generate high-temperature steam, is pumped high-temperature steam by aspiration pump 24
Culture medium is stirred into fermentation tank 6, while by stirring blade 30 so that it can be come into full contact with high-temperature steam, simultaneously
The tail gas in fermentation tank 6 is extracted out by air pump 10.
The present invention can be operated easily, and occupied space is few when use, easy to operate and use；It can be quickly to training
It supports base and carries out sterilization treatment, improve ease of use；The setting of electric heating sheets 28 facilitates the generation of high-temperature steam, improves
Ease of use.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (10)
1. a kind of cell culture medium automatic fermenter with high dispersive sterilizing, including pedestal (1), it is characterised in that：It is described
The upper surface left end of pedestal (1) is equipped with liquid storing barrel (19), is equipped with positioning column in the middle part of the upper surface of liquid storing barrel (19), positioning column it is upper
End face is equipped with steam generating box (18), and the medial surface lower end of steam generating box (18) is equipped with electric heating sheets (28), steam generating box
(18) upper surface is equipped with liquid pump (27), the lateral surface lower end phase that the inlet of liquid pump (27) passes through conduit and liquid storing barrel (19)
Even, the liquid outlet of liquid pump (27) is connected by the 4th diversion pipe (26) with the upper surface of steam generating box (18), the 4th diversion pipe
(26) lateral surface is equipped with the 4th water conservancy diversion tube valve, the surface of pedestal (1) close to one end of steam generating box (18) upper surface
Equipped with fermentation tank (6), it is equipped with isocon (22) on the left of the upper surface of fermentation tank (6), the lower face of isocon (22) passes through circulation
Pipe extends to the medial surface bottom of fermentation tank (6), passes through third diversion pipe (25) and the 4th in the middle part of the lateral surface of isocon (22)
It is connected in the middle part of the lateral surface of diversion pipe (26), third diversion pipe (25) is equipped with third water conservancy diversion tube valve, steam generating box (18)
Right side be equipped with aspiration pump (24), the right side upper end that the air inlet of aspiration pump (24) passes through conduit and steam generating box (18)
It is connected, the gas outlet of aspiration pump (24) is connected by the second diversion pipe (23) with the lateral surface lower end of isocon (22), and second leads
Flow tube (23) is equipped with the second water conservancy diversion tube valve, and stirring motor (15), stirring motor are equipped in the middle part of the upper surface of fermentation tank (6)
(15) output shaft is equipped with agitating shaft (20), and the lateral surface of agitating shaft (20) is equipped with stirring blade (30), the inside of fermentation tank (6)
Face upper end is equipped with temperature sensor (21), is equipped with supporting rack (35) on rear side of the upper surface middle part of pedestal (1), supporting rack (35) it is upper
End face is equipped with Rose Box (34), and the upper surface of Rose Box (34) is equipped with blower pump (32), and the air inlet of blower pump (32) is by leading
Pipe is connected with the leading flank of Rose Box (34), and the gas outlet of blower pump (32) passes through the 5th diversion pipe (31) and isocon (22)
Lateral surface upper end is connected, and the 5th diversion pipe (31) is equipped with the 5th water conservancy diversion tube valve, and drying is equipped on the left of the upper surface of pedestal (1)
Case (13), microcontroller (9) and air pump (10), drying box (13) are internally provided with drier, and the leading flank of drying box (13) is equipped with
The left side of seal cover board (8), drying box (13) is connected equipped with the first diversion pipe (14) with the upper surface of fermentation tank (6), and first
Diversion pipe (14) is equipped with the first water conservancy diversion tube valve, the right side phase that the air inlet of air pump (10) passes through conduit and drying box (13)
Even, the input terminal of microcontroller (9) is electrically connected with the output end of external power supply, the output end of microcontroller (9) respectively with air pump (10),
The input terminal electrical connection of stirring motor (15), aspiration pump (24), liquid pump (27), electric heating sheets (28) and blower pump (32), temperature
Sensor (21) and microcontroller (9) are bi-directionally connected.
2. a kind of cell culture medium automatic fermenter with high dispersive sterilizing according to claim 1, feature exist
In：The pedestal (1) is socketed with screw rod (3) by being disposed thereon the location hole on surface, and the quantity of screw rod (3) is four,
Four screw rods (3) are evenly distributed on the upper surface surrounding of pedestal (1), and the lower face of screw rod (3) is equipped with non-slipping block (2).
3. a kind of cell culture medium automatic fermenter with high dispersive sterilizing according to claim 2, feature exist
In：Threaded one end of the lateral surface of the screw rod (3) close to pedestal (1) upper surface is connected with the first nut, the outside of screw rod (3)
Threaded one end of the face close to pedestal (1) lower surface is connected with the second nut.
4. a kind of cell culture medium automatic fermenter with high dispersive sterilizing according to claim 1, feature exist
In：The lateral surface front end of the liquid storing barrel (19) is equipped with observation window of liquid level mouth (4) and liquid-feeding tube, and liquid-feeding tube is equipped with liquid feeding pipe valve
Door.
5. a kind of cell culture medium automatic fermenter with high dispersive sterilizing according to claim 1, feature exist
In：Be equipped with locating ring (17) in the middle part of the lateral surface of the fermentation tank (6), the lower surface of locating ring (17) by supporting rod (5) with
The upper surface of pedestal (1) is connected.
6. a kind of cell culture medium automatic fermenter with high dispersive sterilizing according to claim 5, feature exist
In：The quantity of the supporting rod (5) is three, and three supporting rods (5) are evenly distributed on the lower surface surrounding of locating ring (17).
7. a kind of cell culture medium automatic fermenter with high dispersive sterilizing according to claim 1, feature exist
In：Charge pipe (16) is equipped on rear side of the upper surface of the fermentation tank (6), and the quantity of charge pipe (16) is three, three chargings
It is equipped with charging tube valve on pipe (16).
8. a kind of cell culture medium automatic fermenter with high dispersive sterilizing according to claim 1, feature exist
In：Probe tube (7) is equipped on front side of the lateral surface lower end of the fermentation tank (6), probe tube (7) is equipped with sampling tube valve, fermentation
The lower face of tank (6) is equipped with drainage conduit (29), and drainage conduit (29) is equipped with discharge tube valve.
9. a kind of cell culture medium automatic fermenter with high dispersive sterilizing according to claim 1, feature exist
In：The medial surface of the Rose Box (34) is equipped with filtering dust net (33), and the left side of Rose Box (34) is equipped with maintenance window, mistake
The trailing flank of filter box (34) is equipped with air-inlet grille.
10. a kind of cell culture medium automatic fermenter with high dispersive sterilizing according to claim 1, feature exist
In：The upper surface left end of the pedestal (1) is equipped with storage tank (12), and storage tank (12) is by being arranged the hinge in its leading flank right end
Chain is rotatably connected to front shroud (11), and left end is equipped with handle in the middle part of the leading flank of front shroud (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810630983.5A CN108456627B (en) | 2018-06-19 | 2018-06-19 | Full-automatic fermentation tank of cell culture medium with high dispersion sterilization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810630983.5A CN108456627B (en) | 2018-06-19 | 2018-06-19 | Full-automatic fermentation tank of cell culture medium with high dispersion sterilization |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108456627A true CN108456627A (en) | 2018-08-28 |
| CN108456627B CN108456627B (en) | 2023-05-09 |
Family
ID=63214882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810630983.5A Active CN108456627B (en) | 2018-06-19 | 2018-06-19 | Full-automatic fermentation tank of cell culture medium with high dispersion sterilization |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108456627B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112300899A (en) * | 2020-11-10 | 2021-02-02 | 衡阳市凯信化工试剂股份有限公司 | Fermentation device for processing chemical reagent |
| CN112941046A (en) * | 2021-02-09 | 2021-06-11 | 珠海高新区维得力生物工程有限公司 | Production process and production equipment of high-activity and high-specificity fructosyltransferase |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2032732C1 (en) * | 1992-02-19 | 1995-04-10 | Валерий Васильевич Григорьев | Device for cleaning waste gas from fermenter |
| US5569605A (en) * | 1993-12-16 | 1996-10-29 | The Chubu Electric Power Co., Inc. | Organic waste disposal system |
| CN203593758U (en) * | 2013-12-20 | 2014-05-14 | 湖北民族学院 | Efficient bioreactor |
-
2018
- 2018-06-19 CN CN201810630983.5A patent/CN108456627B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2032732C1 (en) * | 1992-02-19 | 1995-04-10 | Валерий Васильевич Григорьев | Device for cleaning waste gas from fermenter |
| US5569605A (en) * | 1993-12-16 | 1996-10-29 | The Chubu Electric Power Co., Inc. | Organic waste disposal system |
| CN203593758U (en) * | 2013-12-20 | 2014-05-14 | 湖北民族学院 | Efficient bioreactor |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112300899A (en) * | 2020-11-10 | 2021-02-02 | 衡阳市凯信化工试剂股份有限公司 | Fermentation device for processing chemical reagent |
| CN112941046A (en) * | 2021-02-09 | 2021-06-11 | 珠海高新区维得力生物工程有限公司 | Production process and production equipment of high-activity and high-specificity fructosyltransferase |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108456627B (en) | 2023-05-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206578106U (en) | The glassware cleaning means of Physical Chemistry Experiment | |
| CN209006268U (en) | A kind of medical instrument circulation cleaning device | |
| CN208098824U (en) | A kind of test tube clearing and drying device | |
| CN108456627A (en) | A kind of cell culture medium automatic fermenter with high dispersive sterilizing | |
| CN211035916U (en) | Degassing unit of fermentation cylinder | |
| CN209784111U (en) | full-automatic fiber component analyzer | |
| CN208667675U (en) | A kind of cell culture medium automatic fermenter with high dispersive sterilizing | |
| CN215992273U (en) | Plant tissue culture rotating tube apparatus disinfection device | |
| CN111281803A (en) | Pretreatment method of traditional Chinese medicinal materials | |
| CN212999941U (en) | Cross-linked sodium hyaluronate reaction extraction tank | |
| CN205362154U (en) | High school student uses chemistry experiment test tube washing processing apparatus | |
| CN212976234U (en) | Slide glass cleaning and sterilizing device for biological teaching | |
| CN210728227U (en) | Chinese-medicinal material pretreatment equipment | |
| CN209442782U (en) | A kind of textile printing and dyeing wastewater processing equipment | |
| CN207721169U (en) | A kind of water-bath cleaning machine for fruit cleaning | |
| CN208238454U (en) | Cleaning drying integral machine is used in a kind of production of desiccant | |
| CN213060791U (en) | Sterilizing equipment is used in muddy wine processing | |
| CN212320312U (en) | Reagent tube drying device for gene detection | |
| CN206728925U (en) | A kind of cultivation apparatus with automatic cleaning function filter | |
| CN210226861U (en) | Control by temperature change fodder curing device | |
| CN219309598U (en) | Colorimetric tube cleaning and drying integrated device | |
| CN214321088U (en) | Medical instrument belt cleaning device | |
| CN220846018U (en) | Low-temperature fermentation tank for yellow rice wine | |
| CN217726444U (en) | Combined type citrus fruit residue drying box | |
| CN220507533U (en) | Deodorization type kitchen waste drying mechanism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |