CN104031835B - The replaceable nutrient solution cell micro-gravity system of three-dimensional revolution - Google Patents
The replaceable nutrient solution cell micro-gravity system of three-dimensional revolution Download PDFInfo
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Abstract
The present invention relates to the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution, it comprises: cell culture fluid unit, cell culture fluid permute unit and control unit; Cell culture fluid unit controls driving first motor by control unit and sways; Cell culture fluid permute unit controls the second motor by control unit and rotates with frequency coordination around axle and the first motor coaxle; When the second motor rotates with frequency coordination around axle and the first motor coaxle, make cell exchange liquid go out pipe and cell exchange liquid to enter pipe and aim at cell exchange liquid simultaneously and enter mouth and cell exchange liquid relief outlet, under hydraulic pressure unit drives, make cell exchange liquid go out pipe and cell exchange liquid to enter pipe and insert cell exchange liquid simultaneously and enter mouth and cell exchange liquid relief outlet, then, cell culture fluid permute unit departs from cell culture fluid unit.It automatically can change nutrient solution, the cell simulated microgravity rotary system that when ensureing that long time-histories is turned round, cell has enough nutrients, disturbs from meta-bolites.
Description
Technical field
The present invention relates to a kind of biomedicine experiment equipment, particularly the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution.
Background technology
In recent years along with the fast development of Space Science and Technology, the step that the mankind explore to deep space strides forward day by day.Meanwhile, around various biological effect and the physiological processes of (particularly weightlessness/microgravity environment) Living organism in space environment, the demand more furtherd investigate from cell levels, molecular biology horizontal development is also in continuous increase.But realistic problem is, spaceship, space shuttle and space station technical qualification are limited, and cell biological sample carries with high costs, spacefarer is also usually needed to coordinate in midway manual operation, just can obtain ideal experimental data, so implement difficulty greatly, feasibility is not high.So only rely in true space environment and carry out Cell Biology Experiment, the impact of research weightlessness/microgravity is unpractical, and we need searching and develop can at the mode of ground simulation weightlessness/microgravity environment culturing cell and plant and instrument.
Based on such demand, gyrator (Clinostat) is this ancient and simple laboratory apparatus is subject to people's attention again.Traditional gyrator rotates around own level axle, and is furnished with and can loads by the platform of test agent (plate-like or cylindrical shape).First gyrator invented (VonSachsFGJR.UeberAusschliessungdergeotropischenundhelio troposchenKr ü mmungenw renddesWachsthums.W ü rzburgerArbeiten by German botanist Feng saxophone in 1879 in the world, 2:209-225,1879), " geotropism " phenomenon studying plant roots and stems is mainly used at first.Afterwards in order to can better the demand of base simulated weightlessness/microgravity experiment contentedly, also there is a series of differentiation in the structure of gyrator, function and manufacture craft: from early stage manual, gear drive to electronic, from single shaft to twin shaft, from 2 d-to-3 d, from constant speed to speed change, from without on-line monitoring to can microscopic examination etc., of a great variety, pattern is different.On gyrator, although biological specimen is still in gravity field, be subject to constant gravitational vector effect.But due to the rotation of gyrator, the direction of motion of the biological specimen that it carries constantly changes, have little time all the time to make response (often kind of biology has a minimum impression time or title time of response threshold value to gravity) to the gravity on certain orientation.Gravitational vector shows as equalization, and vector, close to " zero ", i.e. so-called " zero gravity ", thus shows the biological effect of simulated weightlessness/microgravity.
Three-dimensional gyrator structures is designed with two separate pivoted arms, drives inside casing and housing to rotate respectively.In in working process, the rotating speed of housing, turning direction are all random variation, and the biological specimen being placed on inside casing has little time to respond gravity, and the effect of similar spaces microgravity environment appears in process of growth.In the middle of practice, on three-dimensional gyrator, comparatively single shaft two dimension gyrator is more random, so think that the simulated weightlessness effect of this instrument is better for the process of biological specimen gravitational vector equalization.The equivalent universal gravity constant of general three-dimensional gyrator can reach 10
-5~ 10
-3g.Early 1990s, the municipal university of Osaka, Japan protects the grand professor of enjoying of honor and takes the lead in using three-dimensional gyrator simulated microgravity effect (HosonT, KamisakaS, MasudaY, YamashitaM.Changesinplantgrowthprocessesundermicrogravit yconditionssimulatedbyathree-dimentionalclinostat.BotMag, 105:53-70,1992).Three-dimensional gyrator through European Space Agency's improvement in 1997, also known as " random position instrument (RandomPositioningMachine; RPM) ", formally to come into operation (MeslandDA.Novelground-basedfacilitiesforresearchintheeff ectsofweight.ESAMicrogravityNews at Dutch spacelab, 9:5-10,1996), studied by the cell biocompatibility of multinational laboratory applications in simulated weightlessness gradually subsequently.Be numbered in " double-shaft driving frame type gyroscope " patent application of CN101021517 disclosed in China in August, 2007 and also describe intimate device with RPM.
Three-dimensional gyrator is simple to operate, stable performance, gravitational vector meaning process evenly, and adherent or suspension cell can be cultivated in enormous quantities.But cell culture medium can not be changed in the revolution cabin of this gyrator in experimentation, limit its range of application in simulated weightlessness research.Under normal cultivation conditions, cell obtains the nutritive substances such as protein, amino acid, glucose, somatomedin from nutrient solution, also can constantly be discharged in nutrient solution by the meta-bolites of cell itself simultaneously, the composition of nutrient solution and pH value etc. are changed.So, in order to maintain the good growth conditions of cell, generally need 2 ~ 3d to change fresh culture for it.And utilize existing three-dimensional gyrator stimulated microgravity culturing cell, loading the revolution cabin of cell sample needs to be airtight, to be full of a nutrient solution complete body.In order to can microgravity state be remained in turning course, prevent outside air from overflowing into, avoid bubble shearing force to affect cellularity, nutrient solution in cabin can not be changed in experimentation.Be in this situation for a long time, the nutritional status of the hydrostaticpressure in culture vessel and Growth of Cells will be caused to change.Searching document is not difficult to find, the view-point of cell levels revolution simulation weightless test is only selected in 24h, 48h, 72h, the time-histories that these incubation times are relatively short usually.To the longer time-histories microgravity state of research on the impact of organism function, the restriction can not will changed by nutrient solution, cell not only can not get necessary nutrient, also to be easy to by meta-bolites and long-time anoxic affect, and the comprehensive action of these unfavorable factors certainly will the interpretation of interference experiment result.But, there is not yet the method that effectively can solve this problem up to now both at home and abroad and be disclosed report.
Summary of the invention
The object of this invention is to provide a kind of gyrator continue rotate, keep microgravity state under, automatically can change cell culture fluid, when ensureing that long time-histories is turned round, cell has enough nutrients, turns round replaceable nutrient solution cell micro-gravity system from the three-dimensional of meta-bolites interference.
Object of the present invention realizes in the following manner, and the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution, is characterized in that: at least comprise: the integrated support frame that cell culture fluid unit, cell culture fluid permute unit, tape spool rotate and control unit; First control unit records the starting position that cell exchange liquid relief outlet or cell exchange liquid enter mouth, the first motor that control unit controls in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, is entered the real time position of mouth by output step-length record cell exchange liquid relief outlet or cell exchange liquid; Control unit makes cell culture fluid unit and cell culture fluid permute unit pivot by the overall drive motor controlling tape spool rotation, makes cell culture fluid permute unit form Three dimensional rotation;
When changing cell culture fluid, in control unit record cell culture fluid permute unit, cell exchange liquid goes out pipe and cell exchange liquid enters the starting position of pipe; Control unit controls the second motor in cell culture fluid permute unit and rotates with acceleration, cell exchange liquid relief outlet and cell exchange liquid is made to go out to manage by speed governing, cell exchange liquid enters mouth and cell exchange liquid and enters pipe and carry out exactitude position, aligning accuracy depends on that step-length designs, cell exchange liquid relief outlet and cell exchange liquid is made to go out pipe with one heart, cell exchange liquid enters mouth and cell exchange liquid enters pipe with one heart, works under simulated microgravity working order simultaneously;
Control unit is by controlled in wireless hydraulic work system, the oil of the first oil storage chamber is sucked to the oiling of hydro-cylinder cavity of resorption by the first oil-pressure pump, make cell exchange liquid go out pipe insertion cell exchange liquid relief outlet by cylinder body moves, make cell exchange liquid enter pipe insertion cell exchange liquid and enter mouth; By cell exchange liquid oil-pressure pump, cell exchange liquid is inserted cell culture fluid unit from cell exchange sap cavity, and the cell culture fluid that the time is long is put out to putting out cell exchange sap cavity, until the cell culture fluid that the time of having changed is longer, then, cell culture fluid permute unit departs from cell culture fluid unit.
Described cell culture fluid unit comprises: the first motor, cell culture fluid housing, cell rack, cell culture fluid, cell exchange liquid relief outlet, cell exchange liquid enter mouth, the first motor shaft; Cell culture fluid shell one end fixes the first motor, and the first motor-shaft extending, in cell culture fluid housing, axle is socketed with cell rack, and cell rack is placed with cell sample, and cell sample is immersed in cell culture fluid; Cell culture fluid housing lower end has cell exchange liquid relief outlet and cell exchange liquid to enter mouth, enters mouth for inputting cell exchange liquid, and relief outlet is used for the longer cell culture fluid of efflux time; First motor adopts stepper-motor, is entered the position of mouth, use when working asynchronously for cell culture fluid permute unit during rotation by control unit by step-length record, storage cell exchange liquid relief outlet or cell exchange liquid.
Described cell rack has the cell frame of multiple placement cell sample simultaneously, and cell frame is in cell culture fluid, and during the first motor shaft work, cell frame and cell rack together rotate.
Described cell culture fluid permute unit comprises: cell exchange liquid housing, hydro-cylinder cavity of resorption, oil-pressure pump, the first oil storage chamber, the second motor shaft, the second motor, the second oil storage chamber, external slideway, hydro-cylinder epicoele, put out cell exchange sap cavity, cell exchange sap cavity, inner slide, cell exchange liquid go out pipe, cell exchange liquid relief outlet, cell exchange liquid enter pipe, cell exchange liquid enters mouth, the first motor shaft; Cell exchange liquid housing is limited in external slideway and inner slide, cell exchange liquid housing has symmetrical puts out cell exchange sap cavity and cell exchange sap cavity, cell exchange sap cavity upper end fixed cell displacement liquid oil-pressure pump, the outlet of cell exchange liquid oil-pressure pump connects cell exchange liquid and enters pipe; Putting out cell exchange sap cavity upper end has cell exchange liquid to go out pipe; Cell exchange liquid goes out pipe and cell exchange liquid, and to enter pipe symmetrical around the second motor shaft.
Described put out cell exchange sap cavity and there is a hydraulic efficiency system cell exchange sap cavity lower end, hydro-cylinder cavity of resorption in hydraulic efficiency system and hydro-cylinder epicoele communicate with the first oil storage chamber and the second oil storage chamber respectively by the first oil-pressure pump, the second oil-pressure pump, by hydro-cylinder cavity of resorption and epicoele oiling and emptying, make to put out cell exchange sap cavity and cell exchange sap cavity moves up and down along external slideway and inner slide, guarantee to put out cell exchange sap cavity, cell exchange sap cavity steadily changes cell culture fluid.
Described hydro-cylinder cavity of resorption and hydro-cylinder epicoele, the first oil storage chamber and the second oil storage chamber, all near the second motor shaft.
The cell exchange liquid of described cell exchange sap cavity is more than 2 times of cell culture fluid, changes completely to make nutrient solution.
The integrated support frame that described tape spool rotates comprises: the 3rd motor shaft, integrated support frame, bearing, back shaft; Housing is fixedly connected with back shaft, and back shaft is fixed on integrated support frame by left and right bearing, and integrated support frame and basis are fixed, and the output shaft of the 3rd motor shaft is coaxially connected with back shaft; 3rd motor shaft works under control unit controls, and the output shaft of the 3rd motor shaft drives back shaft to rotate, and back shaft drives housing into rotation, and the cell culture fluid unit on housing, cell culture fluid permute unit rotate around back shaft with housing; The first motor that control unit controls in cell culture fluid unit simultaneously rotates under simulated microgravity working order with a certain frequency f, makes cell culture fluid permute unit form Three dimensional rotation.
Advantage of the present invention is: the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution comprises: cell culture fluid unit, cell culture fluid permute unit and control unit, cell culture fluid unit controls driving first motor by control unit and sways, cell culture fluid permute unit controls the second motor by control unit and rotates with frequency coordination around axle and the first motor coaxle, when the second motor rotates with frequency coordination around axle and the first motor coaxle, make cell exchange liquid go out pipe and cell exchange liquid to enter pipe and aim at cell exchange liquid simultaneously and enter mouth and relief outlet, under hydraulic pressure unit drives, make cell exchange liquid go out pipe and cell exchange liquid to enter pipe and insert cell exchange liquid simultaneously and enter mouth and relief outlet, by cell exchange liquid oil-pressure pump, cell exchange liquid is inserted cell culture fluid unit from cell exchange sap cavity, and the cell culture fluid that duration of service is long is put out to putting out cell exchange sap cavity, until changed the cell culture fluid of length duration of service, then, cell culture fluid permute unit departs from cell culture fluid unit.This invention can under gyrator continues the state of rotating, and automatically change cell culture fluid, when ensureing that long time precise integration microgravity is turned round, cell has enough nutrients, disturbs from meta-bolites.
Accompanying drawing explanation
Below in conjunction with embodiment, the present invention is specifically described:
Fig. 1 is embodiment of the present invention schematic diagram;
Fig. 2 is the hydraulic system structure figure of Fig. 1.
In figure, 1, housing; 2, the first motor; 3, cell culture fluid housing; 4, cell rack; 5, cell culture fluid; 6, cell exchange liquid oil-pressure pump; 7, cell exchange liquid housing; 8, hydro-cylinder cavity of resorption; 9, the first oil-pressure pump; 10, the first oil storage chamber; 11, the second motor shaft; 12, the second motor; 13, the second oil storage chamber; 14, external slideway; 15, hydro-cylinder epicoele; 16, cell exchange sap cavity is put out; 17, cell exchange sap cavity; 18, inner slide; 19, cell exchange liquid goes out pipe; 20, cell exchange liquid relief outlet; 21, cell exchange liquid enters pipe; 22, cell exchange liquid enters mouth; 23; First motor shaft; 24, control unit; 25, the second oil-pressure pump; 26, the 3rd motor shaft; 27, integrated support frame; 28, bearing; 29, back shaft.
Embodiment
As shown in Figure 1, the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution, at least comprises: the integrated support frame that cell culture fluid unit, cell culture fluid permute unit, tape spool rotate and control unit 24; First control unit 24 records the starting position that cell exchange liquid relief outlet 20 or cell exchange liquid enter mouth 22, the first motor that control unit 24 controls in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, is entered the work real time position of mouth 22 by output step-length record cell exchange liquid relief outlet 20 or cell exchange liquid; Control unit 24 makes cell culture fluid unit and cell culture fluid permute unit pivot by the overall drive motor controlling tape spool rotation, makes cell culture fluid permute unit form Three dimensional rotation.
When changing cell culture fluid, control unit 24 records cell exchange liquid in cell culture fluid permute unit and goes out the starting position that pipe 19 and cell exchange liquid enter pipe 21; Control unit 24 controls the second motor 12 in cell culture fluid permute unit and rotates with acceleration, cell exchange liquid relief outlet 20 and cell exchange liquid is made to go out pipe 19 by speed governing, cell exchange liquid enters mouth 22 and cell exchange liquid and enters pipe 21 and carry out exactitude position, aligning accuracy depends on that step-length designs, cell exchange liquid relief outlet 20 and cell exchange liquid is made to go out pipe 19 concentric, cell exchange liquid enters mouth 22 and cell exchange liquid enters pipe 21 with one heart, simultaneously under simulated microgravity working order;
Control unit 24 is by controlled in wireless hydraulic work system, the oil of the first oil storage chamber 10 is sucked to hydro-cylinder cavity of resorption 8 oiling by the first oil-pressure pump 9, make cell exchange liquid go out pipe 19 to insert cell exchange liquid relief outlet 20 by cylinder body moves, make cell exchange liquid enter pipe 21 and insert cell exchange liquid and enter mouth 22; By cell exchange liquid oil-pressure pump 6, cell exchange liquid is inserted cell culture fluid unit from cell exchange sap cavity 17, and the cell culture fluid 5 that duration of service is long is put out to putting out cell exchange sap cavity 16, until changed the cell culture fluid of length duration of service, then, cell culture fluid permute unit departs from cell culture fluid unit.
Cell culture fluid unit comprises: the first motor 2, cell culture fluid housing 3, cell rack 4, cell culture fluid 5, cell exchange liquid relief outlet 20, cell exchange liquid enter mouth 22, first motor shaft 23, the first motor 2 is fixed in cell culture fluid housing 3 one end, first motor shaft 23 of the first motor 2 stretches in cell culture fluid housing 3, first motor shaft 23 is socketed with cell rack 4, cell rack 4 is placed with cell sample, cell sample is immersed in cell culture fluid 5, cell culture fluid housing 3 lower end has cell exchange liquid relief outlet 20 and cell exchange liquid to enter mouth 22, cell exchange liquid enters mouth 22 for inputting cell exchange liquid, cell exchange liquid relief outlet 20 is for discharging the cell culture fluid 5 of length duration of service, first motor 2 adopts stepper-motor, during rotation by control unit 24 by step-length record, storage cell exchange liquid relief outlet 20 or cell exchange liquid enter the position of mouth 22, use when working asynchronously for cell culture fluid permute unit.
Cell rack 4 has the cell frame of multiple placement cell sample simultaneously, and cell frame is in cell culture fluid 5, and when the first motor shaft 23 works, cell frame and cell rack 4 together rotate.
When cell exchange liquid oil-pressure pump 6 works, produce and have pressure, pressure makes cell culture fluid produce foam.
Cell culture fluid permute unit comprises: cell exchange liquid housing 7, hydro-cylinder cavity of resorption 8, oil-pressure pump 9, first oil storage chamber 10, second motor shaft 11, second motor 12, second oil storage chamber 13, external slideway 14, hydro-cylinder epicoele 15, put out cell exchange sap cavity 16, cell exchange sap cavity 17, inner slide 18, cell exchange liquid go out pipe 19, cell exchange liquid relief outlet 20, cell exchange liquid enter pipe 21, cell exchange liquid enters mouth 22, first motor shaft 23; Cell exchange liquid housing 7 is limited in external slideway 14 and inner slide 18, what cell exchange liquid housing 7 had a positional symmetry puts out cell exchange sap cavity 16 and cell exchange sap cavity 17, cell exchange sap cavity 17 upper end fixed cell displacement liquid oil-pressure pump 6, the outlet of cell exchange liquid oil-pressure pump 6 connects cell exchange liquid and enters pipe 21; Putting out cell exchange sap cavity 16 upper end has cell exchange liquid to go out pipe 19; Cell exchange liquid goes out pipe 19 and cell exchange liquid, and to enter pipe 21 symmetrical around the second motor shaft 11.
As shown in Figure 2, put out cell exchange sap cavity 16 and there is a hydraulic efficiency system cell exchange sap cavity 17 lower end, hydro-cylinder cavity of resorption 8 in hydraulic efficiency system and hydro-cylinder epicoele 15 communicate with the first oil storage chamber 10 and the second oil storage chamber 13 respectively by the first oil-pressure pump 9, second oil-pressure pump 25, by hydro-cylinder cavity of resorption 8 and hydro-cylinder epicoele 15 oiling and emptying, make to put out cell exchange sap cavity 16 and cell exchange sap cavity 17 moves up and down along external slideway 14 and inner slide 18, keep putting out cell exchange sap cavity 16 and cell exchange sap cavity 17 steadily changes cell culture fluid.
In order to make its cell exchange liquid housing 7 stability of rotation, hydro-cylinder cavity of resorption 8 and hydro-cylinder epicoele 15, first oil storage chamber 10 and the second oil storage chamber 13, all near the second motor shaft 11.
Cell culture fluid permute unit is by the powered battery in housing 1, and cell culture fluid permute unit is communicated by dedicated radio link with control unit 24, independent when cell culture fluid permute unit is worked, and physical condition is not by other structure influence.Control unit is known technology, is not just described in detail here.
The cell exchange liquid of cell exchange sap cavity 17 is more than 2 times of cell culture fluid 5, changes completely to make nutrient solution.
Replacing construction is by displacement detecting or working time control.
Hydro-cylinder is distributed in around the second motor shaft, steady when cell culture fluid is replaced.
The integrated support frame that tape spool rotates comprises: the 3rd motor shaft 26, integrated support frame 27, bearing 28, back shaft 29; Housing 1 is fixedly connected with back shaft 29, and back shaft 29 is fixed on integrated support frame 27 by left and right bearing 28, and integrated support frame 27 and basis are fixed, and the output shaft of the 3rd motor shaft 26 is coaxially connected with back shaft 29; 3rd motor shaft 26 works under control unit 24 controls, the output shaft of the 3rd motor shaft 26 drives back shaft 29 to rotate, back shaft 29 drives housing 1 to rotate around back shaft 29, and the cell culture fluid unit on housing 1, cell culture fluid permute unit rotate around back shaft 29 with housing 1; The first motor that control unit 24 controls in cell culture fluid unit simultaneously rotates under simulated microgravity working order with a certain frequency f, makes cell culture fluid permute unit form Three dimensional rotation.
The parts that the present embodiment does not describe in detail and structure belong to the well-known components of the industry and common structure or conventional means, do not do here and describe one by one.
Claims (8)
1. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution, is characterized in that: at least comprise: the integrated support frame that cell culture fluid unit, cell culture fluid permute unit, tape spool rotate and control unit; First control unit records the starting position that cell exchange liquid relief outlet or cell exchange liquid enter mouth, the first motor that control unit controls in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, is entered the work real time position of mouth by output step-length record cell exchange liquid relief outlet or cell exchange liquid; Control unit makes cell culture fluid unit and cell culture fluid permute unit pivot by the overall drive motor controlling tape spool rotation, makes cell culture fluid permute unit form Three dimensional rotation;
When changing cell culture fluid, in control unit record cell culture fluid permute unit, cell exchange liquid goes out pipe and cell exchange liquid enters the starting position of pipe; Control unit controls the second motor in cell culture fluid permute unit and rotates with acceleration, cell exchange liquid relief outlet and cell exchange liquid is made to go out to manage by speed governing, cell exchange liquid enters mouth and cell exchange liquid and enters pipe and carry out exactitude position, aligning accuracy depends on that step-length designs, cell exchange liquid relief outlet and cell exchange liquid is made to go out pipe with one heart, cell exchange liquid enters mouth and cell exchange liquid enters pipe with one heart, simultaneously under simulated microgravity working order;
Control unit is by controlled in wireless hydraulic work system, the oil of the first oil storage chamber is sucked to the oiling of hydro-cylinder cavity of resorption by the first oil-pressure pump, make cell exchange liquid go out pipe insertion cell exchange liquid relief outlet by cylinder body moves, make cell exchange liquid enter pipe insertion cell exchange liquid and enter mouth; By cell exchange liquid oil-pressure pump, cell exchange liquid is inserted cell culture fluid unit from cell exchange sap cavity, and the cell culture fluid that duration of service is long is put out to putting out cell exchange sap cavity, until changed the cell culture fluid of length duration of service, then, cell culture fluid permute unit departs from cell culture fluid unit.
2. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, is characterized in that: described cell culture fluid unit comprises: the first motor, cell culture fluid housing, cell rack, cell culture fluid, cell exchange liquid relief outlet, cell exchange liquid enter mouth, the first motor shaft; Cell culture fluid shell one end fixes the first motor, first motor-shaft extending of the first motor is in cell culture fluid housing, first motor shaft sleeve is connected to cell rack, cell rack is placed with cell sample, cell sample is immersed in cell culture fluid, cell culture fluid housing lower end has cell exchange liquid relief outlet and cell exchange liquid to enter mouth, and cell exchange liquid enters mouth for inputting cell exchange liquid, and cell exchange liquid relief outlet is for discharging the cell culture fluid of length duration of service; First motor adopts stepper-motor, is entered the position of mouth, use when working asynchronously for cell culture fluid permute unit during rotation by control unit by step-length record, storage cell exchange liquid relief outlet or cell exchange liquid.
3. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 2, it is characterized in that: described cell rack has the cell frame of multiple placement cell sample simultaneously, cell frame is in cell culture fluid, and during the first motor shaft work, cell frame and cell rack together rotate.
4. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, is characterized in that: described cell culture fluid permute unit comprises: cell exchange liquid housing, hydro-cylinder cavity of resorption, the first oil-pressure pump, the first oil storage chamber, the second motor shaft, the second motor, the second oil storage chamber, external slideway, hydro-cylinder epicoele, put out cell exchange sap cavity, cell exchange sap cavity, inner slide, cell exchange liquid go out pipe, cell exchange liquid relief outlet, cell exchange liquid enter to manage, cell exchange liquid enters mouth, the first motor shaft; Cell exchange liquid housing is limited in external slideway and inner slide, cell exchange liquid housing has symmetrical puts out cell exchange sap cavity and cell exchange sap cavity, cell exchange sap cavity upper end fixed cell displacement liquid oil-pressure pump, the outlet of cell exchange liquid oil-pressure pump connects cell exchange liquid and enters pipe; Putting out cell exchange sap cavity upper end has cell exchange liquid to go out pipe; Cell exchange liquid goes out pipe and cell exchange liquid, and to enter pipe symmetrical around the second motor shaft.
5. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 4, it is characterized in that: described put out cell exchange sap cavity and there is a hydraulic efficiency system cell exchange sap cavity lower end, hydro-cylinder cavity of resorption in hydraulic efficiency system and hydro-cylinder epicoele are respectively by the first oil-pressure pump, second oil-pressure pump communicates with the first oil storage chamber and the second oil storage chamber, by hydro-cylinder cavity of resorption and the oiling of hydro-cylinder epicoele and emptying, make to put out cell exchange sap cavity and cell exchange sap cavity moves up and down along external slideway and inner slide, cell exchange sap cavity is put out in maintenance and cell exchange sap cavity steadily changes cell culture fluid.
6. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 5, is characterized in that: described hydro-cylinder cavity of resorption and hydro-cylinder epicoele, the first oil storage chamber and the second oil storage chamber, all near the second motor shaft on position.
7. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, is characterized in that: the cell exchange liquid of described cell exchange sap cavity be cell culture fluid doubly more than, change completely to make nutrient solution.
8. the replaceable nutrient solution cell micro-gravity system of three-dimensional revolution according to claim 1, is characterized in that: the integrated support frame that described tape spool rotates comprises: the 3rd motor shaft, integrated support frame, bearing, back shaft; Housing outside cell culture fluid unit, cell culture fluid permute unit is fixedly connected with back shaft, and back shaft is fixed on integrated support frame by left and right bearing, and integrated support frame and basis are fixed, and the output shaft of the 3rd motor shaft is coaxially connected with back shaft; 3rd motor shaft works under control unit controls, and the output shaft of the 3rd motor shaft drives back shaft to rotate, and back shaft drives housing to rotate around back shaft, and the cell culture fluid unit on housing, cell culture fluid permute unit rotate around back shaft with housing; The first motor that control unit controls in cell culture fluid unit simultaneously rotates under simulated microgravity working order with a certain frequency f, makes cell culture fluid permute unit form Three dimensional rotation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410215232.9A CN104031835B (en) | 2014-05-21 | 2014-05-21 | The replaceable nutrient solution cell micro-gravity system of three-dimensional revolution |
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| CN201410215232.9A CN104031835B (en) | 2014-05-21 | 2014-05-21 | The replaceable nutrient solution cell micro-gravity system of three-dimensional revolution |
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