CN212128190U

CN212128190U - Liquid changing device for multi-cell co-culture simulated weightlessness experiment

Info

Publication number: CN212128190U
Application number: CN202020065913.2U
Authority: CN
Inventors: 胡泽兵; 张舒; 曹新生; 石菲
Original assignee: Fourth Military Medical University FMMU
Current assignee: Fourth Military Medical University FMMU
Priority date: 2020-01-13
Filing date: 2020-01-13
Publication date: 2020-12-11
Anticipated expiration: 2030-01-13

Abstract

The utility model discloses a liquid changing device for a multi-cell co-culture simulated weightlessness experiment, which comprises a gear, a gear rod, a spring stay wire, a spring, a piston, a second transmission mechanism driving the pistons in two liquid changing devices to move synchronously and a second power unit providing power for the second transmission mechanism and the gear rotation; the gear is connected with the gear rod; the piston is arranged in the inner cavity of the cell culture device needing to change liquid; one end of the spring is pressed against the piston, and the other end of the spring is fixed in the inner cavity of the cell culture device needing to change liquid; one end of the spring stay wire is wound on the gear rod, and the other end of the spring stay wire penetrates through the spring to be connected with the piston. Solves the difficult problems of circulation of culture solution and cell secretion in the co-culture of cells growing in different environments and the problem of difficult rotary simulated weightlessness for a long time caused by the consumption of nutrient substances of a culture medium and the accumulation of cell excretion waste in the rotary simulated weightlessness experiment.

Description

Liquid changing device for multi-cell co-culture simulated weightlessness experiment

Technical Field

The utility model belongs to biomechanics experimental facilities field, concretely relates to liquid changing device of simulation weightlessness experiment is cultivateed altogether to cell altogether.

Background

The microgravity environment generated in the aerospace flight can cause a series of changes of a human cardiovascular system, a skeletal muscle system and the like, and the physical health of astronauts is seriously harmed. Therefore, it is very important to study the characteristics and rules of human physiological changes under microgravity, especially to study the mechanism of occurrence at cellular level and molecular level. In view of the limitation of the space flight vehicle and the cost, the micro-gravity effect of the gyrator on the ground level is widely adopted at home and abroad at present. On the gyroscope, the biological sample is still in the gravitational field and is subjected to a constant gravity vector. However, the rotator rotates around the horizontal shaft, so that the moving direction of the biological sample carried by the rotator is changed continuously, and the rotator cannot respond to the gravity in a certain direction all the time, thereby simulating the microgravity biological effect of cells under the condition of aviation flight. The gyrator provides an economic and efficient mode for developing a cell level biological effect and a generation mechanism under a simulated microgravity condition on the ground, but the existing gyrator is limited when being used for a simulated weightlessness experiment under the condition of multi-cell co-culture.

Mutual regulation and control of cells of the same tissue or different tissues through paracrine or long-distance secretion is an important way for the body to exert physiological functions and maintain homeostasis, for example, in the aspect of maintaining bone homeostasis, an interactive regulation and control relationship exists among neovascular endothelial cells, osteoblasts and osteoclasts, and the regulation and control relationship is significantly changed in a microgravity environment. When the main change or source change of which kind of cells has occurred under the microgravity environment is specifically researched so as to influence the functions of other tissues and cells, one kind of cells need to be respectively placed in the simulated weightlessness environment for culturing, and then the influence of cell secretion on the function of another kind of cells which are normally cultured is detected, but the research process cannot be synchronously realized by the existing gyrator. Therefore, a device capable of transferring cell fluid in one simulated environment to another simulated environment was designed.

Disclosure of Invention

For solving exist not enough among the prior art, the utility model aims to provide a liquid changing device of simulation weightless experiment of multicellular coculture has solved two kinds of problems of changing liquid when normal gravity and simulation microgravity coculture.

In order to solve the technical problem, the utility model discloses a following technical scheme realizes:

a liquid changing device for a multi-cell co-culture simulated weightlessness experiment comprises gears, gear rods, spring pull wires, springs, pistons, a second transmission mechanism driving the pistons in two liquid changing devices to synchronously move and a second power unit providing power for the second transmission mechanism and the gears to rotate;

the gear is connected with the gear rod; the piston is arranged in the inner cavity of the cell culture device needing to change liquid; one end of the spring is pressed against the piston, and the other end of the spring is fixed in the inner cavity of the cell culture device needing to change liquid; one end of the spring stay wire is wound on the gear rod, and the other end of the spring stay wire penetrates through the spring and is connected with the piston.

Specifically, the second transmission mechanism comprises a fourth bevel gear, two gear type bearings, a third transmission rod, a fourth transmission rod and a fifth bevel gear;

the gear type bearing is formed by a bearing, a conical surface arranged on an outer ring of the bearing, straight teeth on the conical surface and straight teeth on the bottom surface of the outer ring of the bearing; the gear can be meshed with straight teeth on the bottom surface of the outer ring of the gear type bearing;

fifth bevel gears are respectively arranged at two ends of the third transmission rod, and the fifth bevel gears at two ends of the third transmission rod are respectively meshed with the fourth bevel gear and the straight teeth on the conical surface of one gear type bearing;

the two ends of the fourth transmission rod are respectively provided with a fifth bevel gear, and the fifth bevel gears at the two ends of the fourth transmission rod are respectively meshed with the fourth bevel gear and the straight teeth on the conical surface of the other gear type bearing; and the fourth bevel gear is connected with the second power unit.

Specifically, the gear rod is anchored outside the cell culture device needing liquid replacement through an anchoring shaft sleeve.

Furthermore, the gear brake device also comprises a gear brake rod which generates a braking effect on the gear, one end of the gear brake rod is anchored through a bushing structure, and the other end of the gear brake rod rotates around an anchoring point.

Compared with the prior art, the beneficial effects of the utility model are that:

(1) the utility model discloses a liquid system is traded in the setting for fresh medium flows to the cell culture cavity from the inner tube of a cell culture ware gradually, and old medium gradually returns the inner tube from the cell culture room cavity of another cell culture ware, has solved and has been difficult to carry out the problem of gyration simulation weightlessness for a long time that leads to because of culture medium nutrient substance consumption and cell excretion waste accumulation in the gyration simulation weightlessness experiment.

(2) The cell culture device of the utility model can be respectively arranged on the rotary simulated weightlessness unit and the driven normal gravity unit to respectively culture different cells, and the rotary simulated weightlessness unit generates simulated weightlessness effect by rotating around a horizontal shaft at uniform speed; the normal gravity unit and the rotary simulated weightlessness unit reversely rotate around the vertical shaft at the same speed under the driving of the transmission device, and the gravity condition borne by the cells is not changed; the automatic liquid changing device can slowly control the circulation of a culture medium between the cell culture device on the rotary simulated weightlessness unit and the cell culture device on the normal gravity unit, and solves the problem that two different cells are difficult to co-culture under different gravities (normal gravity and simulated microgravity) in microgravity molecular biology research.

(3) The cell culture chamber partition board is arranged in the cell culture device, so that the cell culture environment is isolated into relatively independent micro spaces, and on one hand, the problem that the conventional gyrator has large volume and few cultured cells, and the cell secretion substances are easily diluted and can not exert normal physiological effect on co-cultured cells is solved; on the other hand, the relatively independent micro-space can minimize the problem that the culture gene in the gyrator has air bubbles to easily generate fluid shear force.

Drawings

Fig. 1 is a schematic view of a liquid changer according to an embodiment of the present invention.

Fig. 2 is a schematic view of the overall structure of the experimental apparatus according to the embodiment of the present invention.

FIG. 3 is a schematic view showing the structure of a cell culture apparatus according to an embodiment of the present invention.

FIG. 4 is a schematic view showing the connection between the inner tube of the cell culture apparatus and the liquid exchange device according to the embodiment of the present invention.

FIG. 5 is a plan view of a cell culture apparatus according to an embodiment of the present invention.

Description of individual reference symbols in the drawings:

1-a rotary simulated weightlessness unit, 2-a normal gravity unit, 3-a cell culture device, 4-a liquid changing device, 5-a first power unit, 6-a first transmission mechanism, 7-a Y-shaped liquid through pipe, 8-a hose, 9-a second transmission mechanism, 10-a second power unit, 11-a bracket, 12-a bearing, 13-a controller, 14-a supporting shaft sleeve and 15-a supporting rod;

101-a first bevel gear, 102-a first mount;

201-second bevel gear, 202-second fixing frame;

301-outer cylinder, 302-inner cylinder, 303-cylinder cover, 304-partition plate, 305-cell climbing sheet, 306-first cavity, 307-second cavity, 308-liquid through hole;

401-gear, 402-gear lever, 403-spring pull, 404-spring, 405-piston, 406-anchoring bushing, 407-gear brake lever;

601-a first transmission rod, 602-a second transmission rod, 603 a third bevel gear;

901-a fourth bevel gear, 902-a gear type bearing, 903-a third transmission rod, 904-a fourth transmission rod, 905-a fifth bevel gear and 906-a fixed rod;

1101-base, 1102-side panel, 1103-top panel.

Detailed Description

The following embodiments of the present invention are given, and it should be noted that the present invention is not limited to the following embodiments, and all the equivalent transformations made on the basis of the technical solution of the present application all fall into the protection scope of the present invention.

Example 1

As shown in fig. 1 and fig. 4, the present embodiment discloses a fluid exchange device for a simulated weightlessness test of multi-cell co-culture, which includes a gear 401, a gear rod 402, a spring pull wire 403, a spring 404, a piston 405, and a second transmission mechanism 9 for driving two fluid exchange devices to move synchronously. The gear rod 402 is supported on the cell culture device 3 needing to change liquid, specifically: both ends of the gear lever 402 are anchored to the cylinder cover 303 of the cell incubator 3 in the above-described embodiment by means of anchoring bosses 406. The gear 401 is connected with a gear rod 402, a piston 405 and a spring 404 are arranged in the first cavity 306, one end of a spring pull wire 403 is wound on the gear rod 402, the other end of the spring pull wire penetrates through the spring 404 to be connected with the piston 405, the piston 405 can slide along the axial direction of the inner cylinder 302 without a gap, and the piston 405 presses the spring 404 when moving towards the gear rod 402. When the gear rod 402 rotates, the spring wire 403 can be wound or unwound, and the piston 405 is controlled to slide in the axial direction of the inner cylinder 302 with the aid of the elastic force of the spring 404.

Through the second conventional mechanism 9, during the liquid changing process, the piston 405 on the liquid outflow side pushes the liquid to flow out, and the piston 405 on the liquid inflow side synchronously pumps the liquid to enter, so that the internal pressure of the liquid is not changed. Specifically, the second transmission mechanism 9 includes a fourth bevel gear 901, two gear type bearings 902, a third transmission rod 903, a fourth transmission rod 904 and a fifth bevel gear 905; the gear bearing 902 is formed by a bearing, a conical surface provided on the outer race of the bearing, straight teeth on the conical surface, and straight teeth on the bottom surface of the outer race of the bearing. The gear 401 can mesh with spur teeth on the bottom surface of the outer ring of the gear bearing 902. The gear bearing 902 of the present embodiment is generally fitted around the cell culture container 3 without interference between the gear bearing 902 and the cell culture container 3, and therefore, the gear bearing 902 has an inner diameter larger than the outer cylinder 301. In the present embodiment, inner rings of two gear type bearings 902 are mounted on the side plate 1102 and the top plate 1103 by fixing rods 906, respectively.

Fifth bevel gears 905 are respectively arranged at two ends of the third transmission rod 903, and the fifth bevel gears 905 at two ends of the third transmission rod 903 are respectively meshed with the fourth bevel gear 901 and bevel teeth on one gear type bearing 902.

Fifth bevel gears 905 are respectively arranged at two ends of the fourth transmission rod 904, and the fifth bevel gears 905 at the two ends of the fourth transmission rod 904 are respectively meshed with the fourth bevel gear 901 and the bevel gear on the other gear type bearing 902. The fourth bevel gear 901 is connected to the second power unit 10, specifically, the second power unit 10 is a motor, and the motor is installed on the bracket 11. Two liquid changing devices are driven to move by a motor. Avoiding the use of multiple motors and increasing control complexity.

By the liquid changing device of the embodiment, fresh culture medium flows from the first cavity 306 of one cell culture device to the cell culture chamber of the second cavity 307 gradually, and old culture medium flows from the second cavity 307 of another cell culture device to the first cavity 306 gradually, so that the metabolic process of the in vivo environment is effectively simulated.

Example 2

The embodiment discloses a cell culture device, as shown in fig. 3 and fig. 5, comprising an outer cylinder 301, an inner cylinder 302, a cylinder cover 303, a partition plate 304 and a slide clamping groove arranged on the partition plate 304 for clamping a cell climbing sheet 305; one end of the outer cylinder 301 is closed, and the other end is open; one end of the inner cylinder 302 is open, the other end is sealed with the cylinder cover 303, and the diameter of the cylinder cover 303 is larger than that of the outer cylinder 301; the inner cylinder 302 is sleeved in the outer cylinder 301, a first cavity 306 is formed in the inner cavity of the inner cylinder 302, a second cavity 307 is formed in the cavity between the inner cylinder 302 and the outer cylinder 301, and the axial length of the inner cylinder 302 is smaller than that of the outer cylinder 301 along the axial direction thereof, so that the outer cylinder 301 and the inner cylinder 302 are communicated; a plurality of partition plates 304 are arranged along the circumference of the outer wall of the inner cylinder 302, and the plurality of partition plates 304 divide the cavity between the outer cylinder 301 and the inner cylinder 302 into a plurality of independent cell culture cavities; the outer wall of the inner barrel 302 is substantially seamless with the cell slide 305.

The cell culture device in the embodiment is divided into a culture solution cavity for placing culture solution and a cell culture cavity for culturing cells, and the culture solution is provided for the cell culture cavity through the culture solution cavity; the cell culture chamber partition plate 304 is arranged in the cell culture device to separate the cell culture environment into relatively independent micro spaces, so that the problem that the conventional gyrator has large volume and few cultured cells and is easy to cause cell secretion substances to be diluted so as not to exert normal physiological effect on co-cultured cells is solved; on the other hand, the relatively independent micro-space can minimize the problem that the culture gene in the gyrator has air bubbles to easily generate fluid shear force.

Example 3

As shown in fig. 1, the embodiment discloses a liquid-changing type simulated weightlessness test device for multi-cell co-culture, which includes a rotary simulated weightlessness unit 1 capable of rotating around a horizontal axis, a normal gravity unit 2 capable of rotating around a vertical axis, a cell culture apparatus 3, a liquid-changing device 4, and a first power unit 5 for driving the rotary simulated weightlessness unit 1 and the normal gravity unit 2 to rotate; wherein, the rotary simulated weightlessness unit 1 generates simulated weightlessness effect, the normal gravity unit 2 does not change the gravity condition of the cells, and the first power unit 5 is a motor.

Both the rotary simulated weightlessness unit 1 and the normal gravity unit 2 are provided with cell culture devices 3, and specifically, the cell culture devices 3 can be installed in the rotary simulated weightlessness unit 1 and the normal gravity unit 2 in a pluggable manner. A first cavity 306 for storing cell culture solution and a second cavity 307 for culturing cells are arranged in the cell culture device 3, and the first cavity 306 is communicated with the second cavity 307; the second cavity 307 on the cell culture vessel 3 in the rotary simulated weight loss unit 1 is in communication with the second cavity 307 on the cell culture vessel in the normal gravity unit 2.

The liquid changing device 4 is arranged in the first cavity 306 of the cell culture device 3, and the liquid changing device 4 can push the cell culture liquid in the first cavity 306 to move, so as to drive the liquid in the cell culture device 3 in the rotary simulated weight loss unit 1 to circulate with the liquid in the cell culture device 3 in the normal gravity unit 2. The liquid changing device 4 can slowly control the circulation of the culture medium between the cell culture device on the rotary simulated weightlessness unit 1 and the cell culture device on the normal gravity unit 2. For example, the culture solution in the rotary simulated weightlessness unit 1 flows to the normal gravity unit 2, so that the cell factors secreted by the cells under simulated weightlessness flow to the cells cultured under normal gravity along with the culture solution, thereby observing the influence on the functions of the cells, and solving the problem that two different cells are difficult to co-culture under different gravities (normal gravity and simulated microgravity) in microgravity molecular biology research;

meanwhile, the liquid changing device 4 enables fresh culture medium to gradually flow to the cell culture chamber from the inner cylinder of one cell culture device, and old culture medium gradually flows back to the inner cylinder from the cell culture chamber of the other cell culture device, so that the problem that the rotary simulated weightlessness is difficult to carry out for a long time due to the consumption of nutrient substances of the culture medium and the accumulation of cell excretion waste in the rotary simulated weightlessness experiment is solved.

In this embodiment, the rotary simulated weight loss unit 1 includes a first bevel gear 101 for driving the cell culture device to rotate and a first fixing frame 102 for supporting and fixing the cell culture device 3, wherein the first bevel gear 101 is a straight bevel gear, and the first fixing frame 102 is a circular frame. The axis of the first bevel gear 101 is along the horizontal direction, and the first bevel gear 101 is connected with the first fixing frame 102, specifically, in this embodiment, the first bevel gear 101 is connected with the first fixing frame 102 by selecting a clamping groove and a fastening manner. Preferably, the rotation simulation weightlessness unit 1 is supported by a bracket 11, a bearing 12 is sleeved outside the first fixing frame 102, and the bearing 12 is fixed on the bracket 11, so that the first fixing frame 102 can rotate around a horizontal shaft on the bracket 11.

In this embodiment, the normal gravity unit 2 includes a second bevel gear 201 for driving the cell culture device 3 to rotate and a second fixing frame 202 for supporting and fixing the cell culture device 3, the axis of the second bevel gear 201 is along the vertical direction, the second bevel gear 201 is connected with the second fixing frame 202, and specifically, the cell culture device 3 is also clamped with the second fixing frame 202 in the form of a clamping groove and a buckle. In the present embodiment, the second holder 202 has the same shape as the first holder 102, and the first bevel gear 201 and the second bevel gear 201 also have the same shape. The normal gravity unit 2 is supported at another position of the bracket 11, and a bearing 12 is sleeved outside the second fixing frame, and the bearing 12 is fixed on the bracket 11, so that the second fixing frame 102 can rotate around a vertical shaft on the bracket 11.

Preferably, the second cavity 307 in the rotation simulated weightlessness unit 1 is communicated with the second cavity 307 in the normal gravity unit 2 through a hose 8, and two ends of the hose 8 are respectively sleeved at the common ends of the Y-shaped liquid through pipes 7 on the sides of the rotation simulated weightlessness unit 1 and the normal gravity unit 2, so that two ends of the hose 8 are respectively positioned on the rotation axes of the rotation simulated weightlessness unit 1 and the normal gravity unit 2, thereby ensuring that the hose 8 only has rotation movement without displacement change in operation.

This embodiment can drive synchronous antiport of gyration simulation weightlessness unit 1 and normal gravity unit 2 respectively through two motors, nevertheless the utility model discloses preferably following scheme:

a first transmission mechanism 6 is connected between the rotary simulated weightlessness unit 1 and the normal gravity unit 2, so that the rotary simulated weightlessness unit 1 and the normal gravity unit 2 reversely rotate at the same speed. Specifically, in this embodiment, as shown in fig. 1, the first transmission mechanism 6 includes a first transmission rod 601, a second transmission rod 602, and a third bevel gear 603, the third bevel gear 603 is respectively disposed at two ends of the first transmission rod 601, the third bevel gear 603 is respectively disposed at two ends of the second transmission rod 602, the first transmission rod 601 is disposed along a horizontal direction, and the second transmission rod 602 is disposed along a vertical direction. The first transmission rod 601 and the second transmission rod 602 are meshed through a third bevel gear 603, and the third bevel gear 603 on the first transmission rod 601 is meshed with the rotation simulation weightlessness unit 1 through a gear, specifically, the first bevel gear 101 is meshed with the third bevel gear 603 on the first transmission rod 601. The third bevel gear 603 on the second transmission rod 602 is in gear engagement with the normal gravity unit 2, in particular the second bevel gear 201 is in gear engagement with the third bevel gear 603 on the second transmission rod 602. The first power unit 5 is connected with a first bevel gear 101 of the rotary simulated weight loss unit 1.

The first driving rod 601 is provided with a supporting sleeve 14, the supporting sleeve 14 is connected with one end of a supporting rod 15, and the other end of the supporting rod 15 is fixed on a top plate 1103 of the bracket 11 or other components for supporting the device. Likewise, the second driving lever 602 is also supported in the side plate 1102 of the bracket 11 by the support boss 14 and the support bar 15. The first power unit 5 is supported on the base 1101 by a support rod 15.

In the embodiment of the present invention, as shown in fig. 2 and 4, the cell culture device 3 includes an outer tube 301, an inner tube 302, a tube cover 303, a partition plate 304, and a slide groove provided on the partition plate 304 for clamping the cell slide 305. One end of the outer cylinder 301 is closed, and the other end is open; the cylinder cover 303 is annular, one end of the inner cylinder 302 is open, the other end of the inner cylinder is sealed with the inner ring of the cylinder cover 303, the diameter of the outer ring of the cylinder cover 303 is larger than that of the outer cylinder 301, the cylinder cover 303 can be detachably connected with the outer cylinder 301, specifically, threads are arranged on the inner wall of the outer ring of the cylinder cover 303, and one end of the outer wall of the outer cylinder 301, which is connected with the cylinder cover 303, is provided with threads, so that the outer ring of the cylinder cover 303 is connected with the outer wall of.

The inner cylinder 302 is sleeved in the outer cylinder 301, a first cavity 306 is formed in an inner cavity of the inner cylinder 302, a second cavity 307 is formed in a cavity between the inner cylinder 302 and the outer cylinder 301, and the axial length of the inner cylinder 302 is smaller than that of the outer cylinder 301 along the axial direction thereof, so that a certain interval exists between the open end of the inner cylinder 302 and the closed end of the outer cylinder 301 after the inner cylinder 302 is sleeved in the outer cylinder 301, and the interval ensures the communication between the first cavity 306 and the second cavity 307.

A plurality of partition plates 304 are arranged on the circumference of the outer wall of the inner cylinder 302, and the cavity between the outer cylinder 301 and the inner cylinder 302 is divided into a plurality of relatively independent cell culture cavities by the plurality of partition plates 304, so that the problem that the conventional gyrator has large volume and few cultured cells, and is easy to cause cell secretion substances to be diluted and cannot exert normal physiological effect is solved; on the other hand, the relatively independent micro-space can minimize the problem that the culture gene in the gyrator has air bubbles to easily generate fluid shear force. The cell slide 305 can be placed in the second cavity 307, and as shown in fig. 4, no gap is formed between the outer wall of the inner cylinder 302 and the cell slide 305, so that a cell culture chamber is formed between the cell slide 305 and the inner wall of the outer cylinder 301.

The cylinder cover 303 is provided with a liquid through hole 308 communicating with the second cavity 307. Based on the utility model discloses a trade structure of liquid device 4, the symmetry sets up two through holes 308 on cover 303, and two through holes 308 are connected with two symmetrical ends of Y type through pipe 7, as shown in fig. 3. The common end of the Y-shaped liquid through pipe 7 on the side of the rotary simulated weightlessness unit 1 is communicated with the common end of the Y-shaped liquid through pipe 7 on the side of the normal gravity unit 2 through a hose 8. The connecting line of the two liquid through holes 308 is in cross distribution with the gear rod, so that the interference between the positions of the gear rod 402 and the liquid through holes 308 is avoided; simultaneously because the second cavity 307 in the simulated weightlessness unit of gyration 1 and the second cavity 307 in the normal gravity unit 2 communicate through a hose 8, through the utility model discloses a structure setting makes the both ends of hose 8 be located the rotation axis of simulated weightlessness unit of gyration 1 and normal gravity unit 2 respectively, and guarantees the simulated weightlessness unit of gyration 1 and normal gravity unit 2 counter-rotation with fast, like this, avoids the hose winding, influences the logical liquid between the two.

In an embodiment, as shown in fig. 2 and 3, the liquid changing device 4 comprises a gear 401, a gear rod 402, a spring pull wire 403, a spring 404 and a piston 405; the gear rod 402 is supported on the cell culture device 3, specifically: both ends of the gear lever 402 are anchored to the cylinder cover 303 of the cell incubator 3 in the above-described embodiment by means of anchoring bosses 406. A piston 405 and a spring 404 are disposed in the first cavity 306, with one end of the spring 404 bearing against the piston 405 and the other end secured to the inner wall of the inner barrel 302. One end of a spring pull wire 403 is wound on the gear rod 402, the other end of the spring pull wire penetrates through a spring 404 and is connected with a piston 405, the piston 405 can slide along the axial direction of the inner cylinder 302 without a gap, and the spring 404 is extruded when the piston 405 moves towards the gear rod 402; when the gear rod 402 rotates, the spring wire 403 can be wound or unwound, and the piston 405 is controlled to slide in the axial direction of the inner cylinder 302 with the aid of the elastic force of the spring 404. Gear 401 is connected with gear lever 402, drives gear 401 rotation through second power unit 10, and under gear 401 drove, gear lever 402 can be rotatory around self center pin.

This embodiment can drive gear 401 in the gear 401 of gyration simulated weightlessness unit 1 and the normal gravity unit 2 respectively through two motors and rotate, nevertheless the utility model discloses preferred following scheme:

a second transmission mechanism 9 is arranged between the liquid changing device 4 on the side of the rotary simulated weightlessness unit 1 and the liquid changing device 4 on the side of the normal gravity unit 2, so that the two gears 401 can rotate at the same speed. In this embodiment the second transmission 9 comprises a fourth bevel gear 901, a gear bearing 902, a third transmission rod 903, a fourth transmission rod 904 and a fifth bevel gear 905.

The gear bearing 902 is formed by a bearing, preferably a deep groove ball bearing, provided on a tapered surface of a bearing outer ring, straight teeth on the tapered surface, and straight teeth on a bottom surface of the bearing outer ring. The gear type bearings 902 are sleeved outside the cell culture device 3, the inner diameter of the gear type bearings 902 is larger than the diameter of the outer cylinder 301, and the inner rings of the two gear type bearings 902 are respectively arranged on the side plates 1102 and the top plate 1103 through fixing rods 906; the center of the circle is coaxial with the center of a bearing 12 at the cell culture device at the side of the rotary simulated weightlessness unit 1 and the center of the circle of the bearing 12 at the cell culture device at the side of the normal gravity unit 2, the inner ring of the gear type bearing 902 is not in contact with the cell culture device 3, and the gear 401 can be meshed with the straight teeth on the bottom surface of the outer ring of the gear type bearing 902; fifth bevel gears 905 are respectively arranged at two ends of the third transmission rod 903, and the fifth bevel gears 905 at two ends of the third transmission rod 903 are respectively meshed with the fourth bevel gear 901 and straight teeth on conical surfaces of the gear type bearing 902 on the side of the rotary simulated weightlessness unit 1; the fifth bevel gears 905 are respectively arranged at two ends of the fourth transmission rod 904, and the fifth bevel gears 905 at two ends of the fourth transmission rod 904 are respectively meshed with the fourth bevel gear 901 and the straight teeth on the conical surfaces of the gear type bearing 902 at the side of the normal gravity unit 2. It should be noted that, since the whole liquid changing device 4 rotates with the cell culture device 3, the rotation speed of the outer ring of the gear bearing 902 needs to be set different from the rotation speed of the rotary simulated weightlessness unit 1 at any time or at any timing, so that the gear 401 engaged with the gear needs to drive the spring 404 to pull the piston 405 to move. At this time, only one power system is needed, that is, the fourth bevel gear 901 is connected with the second power unit 10. Specifically, the second power unit 10 is a motor, the motor is mounted on the bracket 11, and the fourth bevel gear 901 is connected with the motor. Avoiding the use of multiple motors and increasing control complexity.

Preferably, in this embodiment, as shown in fig. 3, a gear brake lever 407 is mounted on the outer side of the top wall of the cylinder cover 303 near the gear 401, and the gear brake lever 407 can rotate around its own anchor point through a bushing structure, so that the free end of the gear brake lever 407 can be engaged on the insection of the gear 401 to exert a braking action. Specifically, in the experiment preparation stage, when the cell culture solution is filled into the cell culture device 3, the gear 401 can be braked by using the gear brake lever 407, so that the piston 405 can be stably stopped at a certain set position of the inner cylinder 302; when the two cell culture devices 3 are prepared and connected, and are inserted into the rotary simulated weightlessness unit 1 and the normal gravity unit 2, and the gear 401 is meshed with the straight teeth on the bottom surface of the outer ring of the gear type bearing 902, the gear brake lever 407 can be removed.

The bracket 11 of this embodiment includes a base 1101, a side plate 1102 and a top plate 1103, the top plate 1103 is provided with a first mounting hole for mounting the normal gravity unit 2, and the side plate 1101 is provided with a second mounting hole for mounting the rotation simulated weightlessness unit 1.

As an optional embodiment of the present invention, the testing apparatus is further provided with a controller 13, the controller 13 can control the rotation speeds of the first power unit 5 and the second power unit 10 respectively, so that the rotation speed can be synchronized or differentially rotated between the outer ring of the gear type bearing 902 on the side of the rotation simulation weightless unit 1 and the first bevel gear 101, and between the outer ring of the gear type bearing 902 on the side of the normal gravity unit 2 and the second bevel gear 201. As long as the rotation speed difference between the first power unit 5 and the second power unit 10 is controlled, the gear autorotation speed on the cell culture device can be accurately controlled, and the slow flow of the cell culture medium between the rotary simulated weightlessness unit and the normal gravity unit can be automatically controlled at the liquid changing speed or interval required by the experiment; in the liquid changing process, the piston 405 on the liquid outflow side can be set to push the liquid to flow out, and the piston 405 on the liquid inflow side synchronously sucks the liquid to enter, so that the internal pressure of the liquid is not changed.

It should be noted that the present invention is not limited to the above embodiments, and based on the technical solutions disclosed in the present invention, those skilled in the art can make some substitutions and modifications to some technical features without creative efforts according to the disclosed technical contents, and these substitutions and modifications are all within the protection scope of the present invention.

Claims

1. A liquid changing device for a multi-cell co-culture simulated weightlessness experiment is characterized by comprising a gear (401), a gear rod (402), a spring pull wire (403), a spring (404), pistons (405), a second transmission mechanism (9) driving the pistons (405) in two liquid changing devices to synchronously move and a second power unit (10) providing power for the rotation of the second transmission mechanism (9) and the gear (401);

the gear (401) is connected with a gear rod (402); the piston (405) is arranged in the inner cavity of the cell culture device needing to change liquid; one end of the spring (404) is pressed against the piston (405), and the other end of the spring is fixed in the inner cavity of the cell culture device needing to change liquid; one end of the spring pull wire (403) is wound on the gear rod (402), and the other end of the spring pull wire passes through the spring (404) and is connected with the piston (405).

2. The liquid changing device for the simulated weightlessness test in multi-cell co-culture according to claim 1, wherein the second transmission mechanism (9) comprises a fourth bevel gear (901), two gear bearings (902), a third transmission rod (903), a fourth transmission rod (904) and a fifth bevel gear (905);

the gear type bearing (902) is formed by a bearing, a conical surface arranged on the outer ring of the bearing, straight teeth on the conical surface and straight teeth on the bottom surface of the outer ring of the bearing; the gear (401) can be meshed with straight teeth on the bottom surface of an outer ring of the gear type bearing (902);

fifth bevel gears (905) are respectively arranged at two ends of the third transmission rod (903), and the fifth bevel gears (905) at two ends of the third transmission rod (903) are respectively meshed with the fourth bevel gear (901) and straight teeth on conical surfaces of one gear type bearing (902);

fifth bevel gears (905) are respectively arranged at two ends of the fourth transmission rod (904), and the fifth bevel gears (905) at two ends of the fourth transmission rod (904) are respectively meshed with straight teeth on conical surfaces of the fourth bevel gear (901) and the other gear type bearing (902); the fourth bevel gear (901) is connected with the second power unit (10).

3. The apparatus for exchanging liquid in simulated weightlessness test in multi-cell co-culture according to claim 1, wherein said gear rod (402) is anchored outside the cell culture apparatus requiring liquid exchange through an anchoring bushing.

4. The fluid exchange device for the multi-cell co-culture simulated weightlessness test according to claim 1, further comprising a gear brake lever (407) for braking the gear (401), wherein one end of the gear brake lever (407) is anchored by a bushing structure, and the other end rotates around the anchor point.