CN203878153U - Culture solution replaceable type long time interval cell microgravity rotation device - Google Patents
Culture solution replaceable type long time interval cell microgravity rotation device Download PDFInfo
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- CN203878153U CN203878153U CN201420260537.7U CN201420260537U CN203878153U CN 203878153 U CN203878153 U CN 203878153U CN 201420260537 U CN201420260537 U CN 201420260537U CN 203878153 U CN203878153 U CN 203878153U
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- cell
- exchange liquid
- cell exchange
- culture fluid
- cell culture
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Abstract
The utility model relates to a culture solution replaceable type long time interval cell microgravity rotation device which is characterized by at least comprising a cell culture liquid unit, a cell culture liquid replacing unit and a control unit, wherein the control unit is used for firstly recording an initial position of a cell replacing liquid outlet or cell replacing liquid inlet, used for controlling a first motor in the cell culture liquid unit to operate in an analog microgravity working state at frequency f, and used for recording a real-time working position of the cell replacing liquid outlet or cell replacing liquid inlet through output step length. The device, in the state that a gyrator rotates continuously and the microgravity is maintained, is a culture solution replaceable type long time interval cell microgravity rotation system which is capable of automatically replacing a cell culture liquid and ensuring that a cell is sufficient in nutrition and is prevented from being interfered by metabolite when the cell rotates in a long time interval.
Description
Technical field
The utility model relates to a kind of biomedicine experiment equipment, particularly has the long time-histories cell of the nutrient solution exchangeable microgravity turning device of cell culture fluid recycling replacing ability.
Background technology
Earthnik's class be not subject to the effect of gravity all the time.In long-term evolutionary process, the mankind have adapted to the 1G gravity environment of earth surface.Yet under space flight weightlessness, hydrostatic pressure disappears, body fluid head is to distribution, cause each physiological system of body and occur a series of adaptations, cardiovascular systems, musculoskeletal system, vestibular nervous system, blood system, immunity system etc. are produced to detrimentally affect, have a strong impact on completing of space travel personnel Space-Work ability and aerial mission, return simultaneously occur that orthostatic tolerance declines behind ground, the degradation symptom of maladjustment again under motor capacity.Along with survey of deep space project in countries in the world is launched, the long time-histories travelling of interplanetary becomes a reality gradually, carries out the correlative studys such as impact of microgravity environment on organism state, significant.
Cell gyrator (Clinostat) is a kind of simulated microgravity device that carries out cell cultures of in recent years developing.Generally, cell gyrator is comprised of several revolutions cabin and related Control System.Revolution cabin can be controlled and at the uniform velocity to rotate around transverse axis according to certain speed through Controlling System, can fixed number piece special slide glass in cabin, and can contain into cell culture fluid.While carrying out the research of cell simulated weightlessness, first culturing cell in Tissue Culture Flask, makes cell climb fully loaded slide, then slide glass is fixed in the revolution cabin of the cell culture fluid that contains fresh configuration, according to fixed speed rotation revolution cabin.In rotary course, keep revolution cabin to be full of completely by cell culture fluid, can not contain any bubble, prevent the impact of bubble to slide surface cell in rotary course.Continue in rotary course, gravity vector constantly changes, and cell can not continue to experience the gravity vector of same direction.It is generally acknowledged that this state and microgravity state produce identical biological effect.This is also to adopt gyrator as the theoretical foundation of simulated weightlessness device.On space station or spaceship, the intensity of gravity is about 10
-4g, cell gyrator, when 30 revs/min of rotations, can produce 10
-3g.This is enough to carry out the correlative study of gravity cytobiology, inquires into the physiological and pathological of organism under microgravity environment and changes and related mechanism, for long time-histories spaceflight provides prevention policies.
Continue in rotary course, revolution cabin is an airtight complete body that is full of cell culture fluid.According to research, need, often need to continue 24h to 48h and turn round, in order to study the impact of long time-histories microgravity state on physiological function, even need the lasting revolution of 72h or longer time.Culturing cell is the nutrition nutrient such as attract proteins, amino acid, glucose, somatomedin in nutrient solution, discharges meta-bolites in nutrient solution simultaneously, and the composition of nutrient solution and pH value etc. are changed.Within 1-2 days, will change cell culture fluid under normal circumstances, to keep culturing cell to have enough nutrients, guarantee that cell state avoids meta-bolites and disturb.When carrying out correlative study, the thorny problem running into is: under cell turn state, in order to keep microgravity state, prevent that gas from entering nutrient solution simultaneously, cannot carry out the replacing of nutrient solution.When observing long time-histories microgravity state (72h or longer) on the affecting of organism function, culturing cell can not obtain necessary nutrient in time, will be subject to the impact of self meta-bolites again, the deciphering that this will interference experiment result.
Utility model content
The purpose of this utility model is to provide a kind of under the lasting revolution of gyrator, maintenance microgravity state, can automatically change cell culture fluid, while guaranteeing long time-histories cell revolution, cell has enough nutrients, avoids the long time-histories cell of the nutrient solution exchangeable microgravity turning device that meta-bolites disturbs.
The purpose of this utility model realizes in the following manner: the long time-histories cell of nutrient solution exchangeable microgravity turning device, is characterized in that: at least comprise: cell culture fluid unit, cell culture fluid permute unit and control unit; First control unit records the starting position that cell exchange liquid relief outlet or cell exchange liquid enter mouthful, the first motor that control unit is controlled in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, by output step-length, records the work real time position that cell exchange liquid relief outlet or cell exchange liquid enter mouthful;
While changing cell culture fluid, control unit records cell exchange liquid in cell culture fluid permute unit and goes out the starting position that pipe and cell exchange liquid enter pipe; Control unit is controlled the second motor in cell culture fluid permute unit and is rotated with acceleration, by speed governing, make cell exchange liquid relief outlet, cell exchange liquid go out pipe, cell exchange liquid enters mouthful and cell exchange liquid enters pipe and carries out exactitude position, aligning accuracy depends on step-length design, make cell exchange liquid relief outlet and cell exchange liquid go out pipe with one heart, cell exchange liquid enters mouth and cell exchange liquid enters pipe with one heart, works under simulated microgravity working order simultaneously;
Control unit is by controlled in wireless hydraulic work system, by the first oil-pressure pump, suck the oil of the first oil storage chamber to the oiling of hydro-cylinder cavity of resorption, by moving on cylinder body, make cell exchange liquid go out pipe insertion cell exchange liquid relief outlet, make cell exchange liquid enter pipe insertion cell exchange liquid and enter mouth; By cell exchange liquid oil-pressure pump, cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity, and long cell culture fluid of time is put out to putting out cell exchange sap cavity, until changed long cell culture fluid of time, then, cell culture fluid permute unit departs from cell culture fluid unit.
Described cell culture fluid unit comprises: the first motor, cell culture fluid housing, cell rack, cell culture fluid, cell exchange liquid relief outlet, cell exchange liquid enter mouth, the first motor shaft, the first motor is fixed in cell culture fluid housing one end, the first motor-shaft extending of the first motor is in cell culture fluid housing, the first motor shaft sleeve is connected to cell rack, cell rack is placed with cell sample, cell sample is invaded in cell culture fluid, cell culture fluid housing lower end has cell exchange liquid relief outlet and cell exchange liquid to enter mouth, cell exchange liquid enters mouth for inputting cell exchange liquid, cell exchange liquid relief outlet is for the long cell culture fluid of efflux time, the first motor adopts stepper-motor, during rotation by control unit by step-length record, storage cell exchange liquid relief outlet or cell exchange liquid enter the position of mouth, while working asynchronously for cell culture fluid permute unit, use.
Described cell rack has the cell frame of a plurality of placement cell samples simultaneously, and cell frame is in cell culture fluid, and during the first motor shaft work, cell frame and cell rack together rotate.
Described cell culture fluid permute unit comprises: cell exchange liquid housing, hydro-cylinder cavity of resorption, oil-pressure pump, the first oil storage chamber, the second motor shaft, the second motor, the second oil storage chamber, external slideway, hydro-cylinder epicoele, put out cell exchange sap cavity, cell exchange sap cavity, inner slide, cell exchange liquid and go out pipe, cell exchange liquid relief outlet, cell exchange liquid and enter that pipe, cell exchange liquid enter mouthful, the first motor shaft; Cell exchange liquid housing is limited in external slideway and inner slide, cell exchange liquid housing has symmetrical cell exchange sap cavity and the cell exchange sap cavity put out, cell exchange sap cavity upper end fixed cell displacement liquid oil-pressure pump, the outlet of cell exchange liquid oil-pressure pump connects cell exchange liquid and enters pipe; Putting out cell exchange sap cavity upper end has cell exchange liquid to go out pipe; Cell exchange liquid goes out pipe and cell exchange liquid, and to enter pipe symmetrical around the second motor shaft.
Put out cell exchange sap cavity and there is a hydraulic efficiency system cell exchange sap cavity lower end, hydro-cylinder cavity of resorption in hydraulic efficiency system and hydro-cylinder epicoele communicate with the first oil storage chamber and the second oil storage chamber by the first oil-pressure pump, the second oil-pressure pump respectively, by hydro-cylinder cavity of resorption and the oiling of hydro-cylinder epicoele and emptying, make to put out cell exchange sap cavity and cell exchange sap cavity moves up and down along external slideway and inner slide, keep putting out cell exchange sap cavity and cell exchange sap cavity is steadily changed cell culture fluid.
Described hydro-cylinder cavity of resorption and hydro-cylinder epicoele, the first oil storage chamber and the second oil storage chamber, hydro-cylinder cavity of resorption and hydro-cylinder epicoele are near the second motor shaft.
The cell exchange liquid of described cell exchange sap cavity is the more than 2 times of cell culture fluid, so that nutrient solution is changed completely.
The utility model has the advantages that: owing to thering is the cell culture fluid system of recycling replacing ability, comprise: cell culture fluid unit, cell culture fluid permute unit and control unit, cell culture fluid unit is controlled and is driven the first motor to sway by control unit, cell culture fluid permute unit is controlled the second motor by control unit and is rotated with frequency coordination around axle and the first motor coaxle, when the second motor around axle and the first motor coaxle when coordination rotates frequently, making cell exchange liquid go out pipe and cell exchange liquid enters pipe and aims at cell exchange liquid simultaneously and enter mouth and cell exchange liquid relief outlet, under hydraulic pressure unit drives, making cell exchange liquid go out pipe and cell exchange liquid enters pipe and inserts cell exchange liquid simultaneously and enter mouth and cell exchange liquid relief outlet, by cell exchange liquid oil-pressure pump, cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity, and long cell culture fluid of time is put out to putting out cell exchange sap cavity, until changed long cell culture fluid of time, then, cell culture fluid permute unit departs from cell culture fluid unit.It can continue revolution at gyrator, keep, under microgravity state, can automatically changing cell culture fluid, and while guaranteeing long time-histories cell revolution, cell has enough nutrients, avoids the cell simulated microgravity rotary system that meta-bolites disturbs.
Accompanying drawing explanation
Below in conjunction with embodiment, the utility model is specifically described:
Fig. 1 is the utility model embodiment schematic diagram;
Fig. 2 is the hydraulic cylinder structure figure of Fig. 1.
In figure, 1, housing; 2, the first motor; 3, cell culture fluid housing; 4, cell rack; 5, cell culture fluid; 6, cell exchange liquid oil-pressure pump; 7, cell exchange liquid housing; 8, hydro-cylinder cavity of resorption; 9, the first oil-pressure pump; 10, the first oil storage chamber; 11, the second motor shaft; 12, the second motor; 13, the second oil storage chamber; 14, the second oil-pressure pump; 15, external slideway; 16, hydro-cylinder epicoele; 17, put out cell exchange sap cavity; 18, cell exchange sap cavity; 19, inner slide; 20, cell exchange liquid goes out pipe; 21, cell exchange liquid relief outlet; 22, cell exchange liquid enters pipe; 23; Cell exchange liquid enters mouth; 24, the first motor shaft; 25, control unit.
Embodiment
As shown in Figure 1, there is the cell culture fluid system of recycling replacing ability, it is characterized in that: at least comprise: cell culture fluid unit, cell culture fluid permute unit and control unit 25; First control unit 25 records cell exchange liquid relief outlet 21 or cell exchange liquid and enters mouthfuls 23 starting position, the first motor that control unit 25 is controlled in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, records cell exchange liquid relief outlet 21 or cell exchange liquid enter mouthfuls 23 work real time position by output step-length;
While changing cell culture fluid, control unit 25 records cell exchange liquid in cell culture fluid permute unit and goes out the starting position that pipe 20 and cell exchange liquid enter pipe 22; Control unit 25 is controlled the second motor 12 in cell culture fluid permute unit and is rotated with acceleration, by speed governing, make cell exchange liquid relief outlet 21, cell exchange liquid go out pipe 20, cell exchange liquid enter mouthfuls 23 and cell exchange liquid enter pipe 22 and carry out exactitude position, aligning accuracy depends on step-length design, make cell exchange liquid relief outlet 21 and cell exchange liquid go out pipe 20 concentric, cell exchange liquid enter mouthfuls 23 and cell exchange liquid to enter pipe 22 concentric, under simulated microgravity working order, work simultaneously;
Control unit 25 is by controlled in wireless hydraulic work system, the oil that sucks the first oil storage chamber 10 by the first oil-pressure pump 9 is to 8 oilings of hydro-cylinder cavity of resorption, by moving on cylinder body, make cell exchange liquid go out pipe 20 insertion cell exchange liquid relief outlets 21, make cell exchange liquid enter pipe 22 insertion cell exchange liquid and enter mouth 23; By cell exchange liquid oil-pressure pump 6, cell exchange liquid is inserted to cell culture fluid unit from cell exchange sap cavity 18, and long cell culture fluid 5 of time is put out to putting out cell exchange sap cavity 17, until changed long cell culture fluid of time, then, cell culture fluid permute unit departs from cell culture fluid unit.Control unit is known technology, is not just described in detail here.
Cell culture fluid unit comprises: the first motor 2, cell culture fluid housing 3, cell rack 4, cell culture fluid 5, cell exchange liquid relief outlet 21, cell exchange liquid enter mouthful 23, first motor shaft 24, the first motor 2 is fixed in cell culture fluid housing 3 one end, the first motor shaft 24 of the first motor 2 stretches in cell culture fluid housing 3, the first motor shaft 24 is socketed with cell rack 4, cell rack 4 is placed with cell sample, cell sample is invaded in cell culture fluid 5, cell culture fluid housing 3 lower ends have cell exchange liquid relief outlet 21 and cell exchange liquid to enter mouth 23, cell exchange liquid enters mouth 23 for inputting cell exchange liquid, cell exchange liquid relief outlet 21 is for the long cell culture fluid 5 of efflux time, the first motor 2 adopts stepper-motor, during rotation by control unit 25 by step-length record, storage cell exchange liquid relief outlet 21 or cell exchange liquid enter the position of mouth 23, while working asynchronously for cell culture fluid permute unit, use.
Cell rack 4 has the cell frame of a plurality of placement cell samples simultaneously, and cell frame is in cell culture fluid 5, and during cell culture fluid cell operation, cell frame and cell rack 4 together rotate.
During 6 work of cell exchange liquid oil-pressure pump, produce and have pressure, pressure to make cell culture fluid can not produce foam.
Cell culture fluid permute unit comprises: cell exchange liquid housing 7, hydro-cylinder cavity of resorption 8, oil-pressure pump 9, the first oil storage chamber 10, the second motor shaft 11, the second motor 12, the second oil storage chamber 13, external slideway 15, hydro-cylinder epicoele 16, put out cell exchange sap cavity 17, cell exchange sap cavity 18, inner slide 19, cell exchange liquid and go out that pipe 20, cell exchange liquid relief outlet 21, cell exchange liquid enter pipe 22, cell exchange liquid enters mouthful 23, first motor shaft 24; Cell exchange liquid housing 7 is limited in external slideway 15 and inner slide 19, cell exchange liquid housing 7 has symmetrical cell exchange sap cavity 17 and the cell exchange sap cavity 18 put out, cell exchange sap cavity 18 upper end fixed cell displacement liquid oil-pressure pumps 6,6 outlets of cell exchange liquid oil-pressure pump connect cell exchange liquid and enter pipe 22; Putting out cell exchange sap cavity 17 upper ends has cell exchange liquid to go out pipe 20; Cell exchange liquid goes out pipe 20 and cell exchange liquid, and to enter pipe 22 symmetrical around the second motor shaft 11.
As shown in Figure 2, put out cell exchange sap cavity 17 and there is a hydraulic efficiency system cell exchange sap cavity 18 lower ends, hydro-cylinder cavity of resorption 8 in hydraulic efficiency system and hydro-cylinder epicoele 16 communicate with the first oil storage chamber 10 and the second oil storage chamber 13 by the first oil-pressure pump 9, the second oil-pressure pump 14 respectively, by hydro-cylinder cavity of resorption 8 and 16 oilings of hydro-cylinder epicoele and emptying, make to put out cell exchange sap cavity 17 and cell exchange sap cavity 18 and move up and down along external slideway 15 and inner slide 19, keep putting out steadily replacing cell culture fluid of cell exchange sap cavity 17 and cell exchange sap cavity 18.
In order to make its cell exchange liquid housing 7 stability of rotation, hydro-cylinder cavity of resorption 8 and hydro-cylinder epicoele 16, the first oil storage chambers 10 and the second oil storage chamber 13, near the second motor shaft 11.
Cell culture fluid permute unit is by the powered battery in housing 1, and cell culture fluid permute unit is electrically connected to and is communicated by wireless with control unit 25, and independent while making the work of cell culture fluid permute unit, physical condition is not subject to other structure influence.
The cell exchange liquid of cell exchange sap cavity 18 is the more than 2 times of cell culture fluid 5, so that nutrient solution is changed completely.
The replacing time can be passed through displacement detecting or working time control.
Hydro-cylinder is distributed in around the second motor shaft, steady while making cell culture fluid displacement.
The parts that the present embodiment does not describe in detail and structure belong to well-known components and common structure or the conventional means of the industry, here not narration one by one.
Claims (7)
1. the long time-histories cell of nutrient solution exchangeable microgravity turning device, is characterized in that: at least comprise: cell culture fluid unit, cell culture fluid permute unit and control unit; First control unit records the starting position that cell exchange liquid relief outlet or cell exchange liquid enter mouthful, the first motor that control unit is controlled in cell culture fluid unit rotates under simulated microgravity working order with a certain frequency f, by output step-length, records the work real time position that cell exchange liquid relief outlet or cell exchange liquid enter mouthful.
2. the long time-histories cell of nutrient solution exchangeable according to claim 1 microgravity turning device, is characterized in that: described cell culture fluid unit comprises: the first motor, cell culture fluid housing, cell rack, cell culture fluid, cell exchange liquid relief outlet, cell exchange liquid enter mouth, the first motor shaft, the first motor is fixed in cell culture fluid housing one end, the first motor-shaft extending of the first motor is in cell culture fluid housing, the first motor shaft sleeve is connected to cell rack, cell rack is placed with cell sample, cell sample is invaded in cell culture fluid, cell culture fluid housing lower end has cell exchange liquid relief outlet and cell exchange liquid to enter mouth, cell exchange liquid enters mouth for inputting cell exchange liquid, cell exchange liquid relief outlet is for the long cell culture fluid of efflux time, the first motor adopts stepper-motor, during rotation by control unit by step-length record, storage cell exchange liquid relief outlet or cell exchange liquid enter the position of mouth, while working asynchronously for cell culture fluid permute unit, use.
3. nutrient solution exchangeable according to claim 2 is grown time-histories cell microgravity turning device, it is characterized in that: described cell rack has the cell frame of a plurality of placement cell samples simultaneously, cell frame is in cell culture fluid, during the first motor shaft work, cell frame and cell rack together rotate.
4. the long time-histories cell of nutrient solution exchangeable according to claim 1 microgravity turning device, is characterized in that: described cell culture fluid permute unit comprises: cell exchange liquid housing, hydro-cylinder cavity of resorption, oil-pressure pump, the first oil storage chamber, the second motor shaft, the second motor, the second oil storage chamber, external slideway, hydro-cylinder epicoele, put out cell exchange sap cavity, cell exchange sap cavity, inner slide, cell exchange liquid and go out that pipe, cell exchange liquid relief outlet, cell exchange liquid enter pipe, cell exchange liquid enters mouth, the first motor shaft; Cell exchange liquid housing is limited in external slideway and inner slide, cell exchange liquid housing has symmetrical cell exchange sap cavity and the cell exchange sap cavity put out, cell exchange sap cavity upper end fixed cell displacement liquid oil-pressure pump, the outlet of cell exchange liquid oil-pressure pump connects cell exchange liquid and enters pipe; Putting out cell exchange sap cavity upper end has cell exchange liquid to go out pipe; Cell exchange liquid goes out pipe and cell exchange liquid, and to enter pipe symmetrical around the second motor shaft.
5. nutrient solution exchangeable according to claim 1 is grown time-histories cell microgravity turning device, it is characterized in that: put out cell exchange sap cavity and there is a hydraulic efficiency system cell exchange sap cavity lower end, hydro-cylinder cavity of resorption in hydraulic efficiency system and hydro-cylinder epicoele are respectively by the first oil-pressure pump, the second oil-pressure pump communicates with the first oil storage chamber and the second oil storage chamber, by hydro-cylinder cavity of resorption and the oiling of hydro-cylinder epicoele and emptying, make to put out cell exchange sap cavity and cell exchange sap cavity moves up and down along external slideway and inner slide, cell exchange sap cavity is put out in maintenance and cell exchange sap cavity is steadily changed cell culture fluid.
6. the long time-histories cell of nutrient solution exchangeable according to claim 1 microgravity turning device, is characterized in that: described hydro-cylinder cavity of resorption and hydro-cylinder epicoele, the first oil storage chamber and the second oil storage chamber, near the second motor shaft.
7. the long time-histories cell of nutrient solution exchangeable according to claim 1 microgravity turning device, is characterized in that: the cell exchange liquid of described cell exchange sap cavity is the more than 2 times of cell culture fluid, so that nutrient solution is changed completely.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420260537.7U CN203878153U (en) | 2014-05-21 | 2014-05-21 | Culture solution replaceable type long time interval cell microgravity rotation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420260537.7U CN203878153U (en) | 2014-05-21 | 2014-05-21 | Culture solution replaceable type long time interval cell microgravity rotation device |
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| CN203878153U true CN203878153U (en) | 2014-10-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420260537.7U Withdrawn - After Issue CN203878153U (en) | 2014-05-21 | 2014-05-21 | Culture solution replaceable type long time interval cell microgravity rotation device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031836A (en) * | 2014-05-21 | 2014-09-10 | 中国人民解放军第四军医大学 | Culture liquid replaceable type long-term cell microgravity rotation system |
-
2014
- 2014-05-21 CN CN201420260537.7U patent/CN203878153U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031836A (en) * | 2014-05-21 | 2014-09-10 | 中国人民解放军第四军医大学 | Culture liquid replaceable type long-term cell microgravity rotation system |
| CN104031836B (en) * | 2014-05-21 | 2015-12-16 | 中国人民解放军第四军医大学 | Nutrient solution exchangeable long time-histories cell microgravity rotary system |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20141015 Effective date of abandoning: 20151216 |
|
| C25 | Abandonment of patent right or utility model to avoid double patenting |