CN107686812A - A kind of incubator of the automatically replaceable nutrient solution of medical experiment - Google Patents
A kind of incubator of the automatically replaceable nutrient solution of medical experiment Download PDFInfo
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- CN107686812A CN107686812A CN201710993956.XA CN201710993956A CN107686812A CN 107686812 A CN107686812 A CN 107686812A CN 201710993956 A CN201710993956 A CN 201710993956A CN 107686812 A CN107686812 A CN 107686812A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
Abstract
The present invention provides a kind of incubator of the automatically replaceable nutrient solution of medical experiment, including fluid reservoir, culturing room, lowering or hoisting gear, liquid changing device, bogey, and PLC；The lowering or hoisting gear includes column, fixed plate, lifting motor, guide pillar, slide, the block that is spirally connected, screw rod and bearing；The liquid changing device includes discharging tube, liquid discharge head, positive displacement pump, liquid-feeding tube, liquid feeding head, charging valve, electronic flowmeter and drip plate；The bogey is located at the bottom of liquid changing device；The bogey includes motor, train of reduction gears, conical gear group, base, bearing, support plate, culture dish, locating piece and locating slot；The setting that the present invention passes through lowering or hoisting gear, liquid changing device and bogey, realize the automatic replacing of cell cultivation process nutrient solution, avoid artificial nutrient solution of changing to hold the time inaccurate and change in liquid the problem of easily causing mistake, significantly reduce and change the liquid time, reduce and change liquid difficulty.
Description
Technical field
The invention belongs to medical experiment equipment technical field, more particularly to a kind of automatically replaceable nutrient solution of medical experiment
Incubator.
Background technology
With the horizontal continuous development of modern science and technology, the everyway applied to medical science realizes intellectuality, but at present
The nutrient solution addition commonly used in medical experiment and substitute mode are still using the method for manually changing liquid so that experimental implementation seems
It is relatively complicated, and easily cause to change the situations such as liquid mistake, nutrient solution pollution in the process, this obvious hair with modern science and technology
Exhibition does not match.Therefore, seem very urgent for the intelligentized research of cell culture.
The Chinese patent that notification number is CN203200259U discloses a kind of cell based on semi-automatic speed governing and changes liquid dress
To put, including change liquid part and speed-regulating switch, the liquid part that changes includes nutrient solution draw-off pump and waste liquid draw-off pump that switching starts,
The import and export of the nutrient solution draw-off pump is connected to the first conduit and the second conduit, the import and export point of the waste liquid draw-off pump respectively
The 3rd conduit and the 4th conduit are not connected to, and the speed-regulating switch is electrically connected by wire and nutrient solution draw-off pump and waste liquid draw-off pump
Connect.The present invention is extracted in fresh medium to culture dish by nutrient solution draw-off pump, and waste liquid draw-off pump is by waste liquid after the completion of culture
Extract out, so as to instead of the artificial mode of operation for changing liquid, improve operating efficiency, in addition nutrient solution draw-off pump and waste liquid draw-off pump
Flow can be adjusted by the rotating speed of speed-regulating switch controlling pump so that change that liquid operation is simpler, changes liquid accuracy
It is higher.Present invention can apply to biological cell culture.
Inventor has found that a kind of cell liquid changing device based on semi-automatic speed governing realizes the artificial mesh for changing liquid of substitution
, but its nutrient solution draw-off pump and waste liquid draw-off pump are only capable of being directed to single culture dish and used, and cause the culture of cell
It is less efficient, limit use.
Notification number is that CN206308374U Chinese patent discloses a kind of fully-automatic intelligent cell culture apparatus, and it includes
Controller, storage chamber, culture chamber, operating cavity and observation chamber, the culture chamber is interior to include the first governor motion and culturing rack,
The cell culture apparatus also includes an isolation ward, first be arranged between culture chamber and isolation ward control door and setting
Second between external environment and isolation ward controls door, also includes the first pipetting device, the isolation ward in affiliated culture chamber
Inside include the second governor motion, the Tissue Culture Flask brought for the first pipetting device of crawl and stretched out by the second control door
The second pipetting device outside to isolation ward, by setting an isolation ward again in fully-automatic intelligent cell culture apparatus, it is used for
The medium that culture chamber is contacted with external environment, thus can avoid staff from directly taking out cultured cell carefully
Outside born of the same parents' culture apparatus, cell culture environment is not interfered with, improves operating efficiency, also improves the training quality of cell.
Inventor has found that a kind of replacing of the fully-automatic intelligent cell culture apparatus for nutrient solution needs the first liquid relief machine
The compounding practices such as structure, the second pipetting device and the second governor motion could be completed to change liquid, and operation is complex, also, it is still
Single culture dish can only be directed to use, limit its practicality.
Then, in view of this, improvement is studied for existing structure and missing, there is provided a kind of medical experiment is used can be certainly
The dynamic incubator for changing nutrient solution, to reach the purpose for having more more practical value.
The content of the invention
In order to solve the above-mentioned technical problem, the present invention provides a kind of culture of the automatically replaceable nutrient solution of medical experiment
Case, to solve existing artificial to change that liquid is cumbersome and easy error, and existing cell culture apparatus operate complex, cell
The problem of efficiency of culture is low.
The purpose and effect of the medical experiment of the present invention incubator of automatically replaceable nutrient solution, by technology hand in detail below
Section is reached：
A kind of incubator of the automatically replaceable nutrient solution of medical experiment, including casing, heat-insulation layer, upper lid, dividing plate, liquid storage
Room, culturing room, heating wire, temperature sensor, waste tank, liquid feeding tank, lowering or hoisting gear, column, fixed plate, lifting motor, lead
Post, slide, be spirally connected block, screw rod, bearing, liquid changing device, discharging tube, liquid discharge head, positive displacement pump, liquid-feeding tube, liquid feeding head, charging valve,
Electronic flowmeter, drip plate, bogey, motor, train of reduction gears, conical gear group, base, bearing, support plate, culture
Ware, locating piece, locating slot, chamber door, observation window and PLC；The madial wall of the casing is provided with heat-insulation layer, the insulation
Layer is heatproof rock wool material；The inside of the casing is separated to form two chambers above and below fluid reservoir and culturing room by dividing plate；It is described
Waste tank and liquid feeding tank are in fluid reservoir；The top of the waste tank and liquid feeding tank is provided with upper lid in casing；Institute
State the bottom that temperature sensor is installed on dividing plate；The outside of the culturing room by hinge on casing in being hinged with chamber door；It is described
Chamber door is provided with observation window；The PLC is on the outer wall of casing；The lowering or hoisting gear is located in culturing room；It is described
Lowering or hoisting gear includes column, fixed plate, lifting motor, guide pillar, slide, the block that is spirally connected, screw rod and bearing；The fixed plate fixes peace
Top side loaded on column；The lifting motor is installed on the top of fixed plate；The clutch end and screw rod of the lifting motor
It is connected；The bottom of the fixed plate is provided with guide pillar at two in the both sides of screw rod；The slide is sheathed on guide pillar；It is described to be spirally connected
Block is sheathed on screw rod；The block that is spirally connected is integral type structure with slide；The right-hand member of the slide is provided with the bearing of integral type；Institute
Liquid changing device is stated to be installed on bearing；The liquid changing device include discharging tube, liquid discharge head, positive displacement pump, liquid-feeding tube, liquid feeding head, plus
Liquid valve, electronic flowmeter and drip plate；The discharging tube and liquid-feeding tube are arranged on bearing；The discharging tube and liquid-feeding tube
Bottom is respectively equipped with liquid discharge head and liquid feeding head；The top of the discharging tube and liquid-feeding tube is connected with waste tank and liquid feeding tank respectively
Connect；The discharging tube is provided with positive displacement pump；The liquid-feeding tube is provided with charging valve and electronic flowmeter；The bogey is located at
The bottom of liquid changing device；The bogey include motor, train of reduction gears, conical gear group, base, bearing, support plate,
Culture dish, locating piece and locating slot；The motor is connected by train of reduction gears with conical gear group；The base
Top is rotatablely connected by bearing and conical gear group；The support plate is fixedly installed on the top of conical gear group；It is described to do
Top array locating piece everywhere is distributed with；The bottom of the culture dish is provided with locating slot；The culture dish is determined by bottom
It is in mating connection that position groove is installed on locating piece.
Further, liquid discharge head and the liquid feeding head is semicircular arc structure.
Further, the bottom of liquid discharge head and the liquid feeding head is equipped with drip plate.
Further, the culture dish is right against liquid discharge head in the position on support plate and liquid feeding head is set.
Further, the observation window is right against culture dish setting on horizontal level.
Further, the discharging tube and liquid-feeding tube be located at holder top one section are the flexible pipe that helical form is set, position
In one section of rest base be plastic rigid pipe.
Further, the S-type structure of the heating wire is wound on the inwall of culturing room.
Further, the charging valve is electric-controlled type valve.
Compared with prior art, the present invention has the advantages that：
1. the present invention realizes cell cultivation process training by the setting of lowering or hoisting gear, liquid changing device and bogey
The automatic replacing of nutrient solution, avoid artificial nutrient solution of changing and the time is held inaccurate and change in liquid and easily cause mistake and ask
Topic, significantly reduces and changes the liquid time, reduce and change liquid difficulty.
2. being provided with culture dish everywhere on the bogey of the present invention altogether, the culture of four groups of cell samples can be realized simultaneously, is carried
The high efficiency of cell culture, strengthen the practicality of the present invention.
3. the culture dish of the present invention is engaged by the groove of bottom with the locating slot on support plate, you can is realized to culture dish
Good fixation, when picking and placeing, and can is conveniently taken.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is external structure schematic diagram of the present invention；
Fig. 3 is lifting device structure schematic diagram；
Fig. 4 is liquid changing device structural representation；
Fig. 5 is liquid discharge head structural representation；
Fig. 6 is bogey structural representation；
Fig. 7 is carrying board structure schematic diagram；
Fig. 8 is culture dish structural representation.
In figure：1- casings, 2- heat-insulation layers, cover on 3-, 4- dividing plates, 5- fluid reservoirs, 6- culturing room, 7- heating wire, 8- temperature
Sensor, 9- waste tanks, 10- liquid feeding tanks, 11- lowering or hoisting gears, 111- columns, 112- fixed plates, 113- lifting motors, 114-
Guide pillar, 115- slides, 116- are spirally connected block, 117- screw rods, 118- bearings, 12- liquid changing devices, 121- discharging tubes, 122- liquid discharge heads,
123- positive displacement pumps, 124- liquid-feeding tubes, 125- liquid feeding heads, 126- charging valves, 127- electronic flowmeters, 128- drip plates, 13- carryings
Device, 131- motors, 132- train of reduction gears, 133- conical gear groups, 134- bases, 135- bearings, 136- support plates,
137- culture dishes, 138- locating pieces, 139- locating slots, 14- chamber doors, 15- observation windows, 16-PLC controllers.
Embodiment
The present invention is described further below in conjunction with accompanying drawing：
Embodiment：
As shown in accompanying drawing 1 to accompanying drawing 8：
A kind of incubator of the automatically replaceable nutrient solution of medical experiment of present invention offer, including casing 1, heat-insulation layer 2, on
Lid 3, dividing plate 4, fluid reservoir 5, culturing room 6, heating wire 7, temperature sensor 8, waste tank 9, liquid feeding tank 10, lowering or hoisting gear 11,
Column 111, fixed plate 112, lifting motor 113, guide pillar 114, slide 115, be spirally connected block 116, screw rod 117, bearing 118, changes liquid
Device 12, discharging tube 121, liquid discharge head 122, positive displacement pump 123, liquid-feeding tube 124, liquid feeding first 125, charging valve 126, electronic flowmeter
127, drip plate 128, bogey 13, motor 131, train of reduction gears 132, conical gear group 133, base 134, bearing
135, support plate 136, culture dish 137, locating piece 138, locating slot 139, chamber door 14, observation window 15 and PLC 16；The case
The madial wall of body 1 is provided with heat-insulation layer 2, and the heat-insulation layer is heatproof rock wool material；The inside of the casing 1 is separated by dividing plate 4
Form fluid reservoir 5 and culturing room two chambers about 6；The waste tank 9 and liquid feeding tank 10 are in fluid reservoir 5；It is described
The top of waste tank 9 and liquid feeding tank 10 is provided with upper lid 3 in casing 1；The temperature sensor 8 is installed on the bottom of dividing plate 4；
The outside of the culturing room 6 by hinge on casing 1 in being hinged with chamber door 14；The chamber door 14 is provided with observation window 15；It is described
PLC 16 is on the outer wall of casing 1；The lowering or hoisting gear 11 is located in culturing room 6；The lowering or hoisting gear 11 includes vertical
Post 111, fixed plate 112, lifting motor 113, guide pillar 114, slide 115, the block 116 that is spirally connected, screw rod 117 and bearing 118；
The fixed plate 112 is fixedly installed in the top side of column 111；The lifting motor 13 is installed on fixed plate 112
Top；The clutch end of the lifting motor 13 is connected with screw rod 117；The bottom of the fixed plate 112 is in screw rod 117
Both sides are provided with guide pillar 114 at two；The slide 115 is sheathed on guide pillar 114；The block 116 that is spirally connected is sheathed on screw rod 117；
The block that is spirally connected is integral type structure with slide 115；The right-hand member of the slide 115 is provided with the bearing 118 of integral type；It is described to change liquid
Device 12 is installed on bearing 118；The liquid changing device 12 includes discharging tube 121, liquid discharge head 122, positive displacement pump 123, liquid-feeding tube
124th, liquid feeding is first 125, charging valve 126, electronic flowmeter 127 and drip plate 128；The discharging tube 121 and liquid-feeding tube 124 are worn
On bearing 118；The bottom of the discharging tube 121 and liquid-feeding tube 124 is respectively equipped with liquid discharge head 122 and liquid feeding first 125；It is described
The top of discharging tube 121 and liquid-feeding tube 124 is connected with waste tank 9 and liquid feeding tank 10 respectively；The discharging tube 121 is provided with
Positive displacement pump 123；The liquid-feeding tube 124 is provided with charging valve 126 and electronic flowmeter 127；The bogey 13 is located at and changes liquid
The bottom of device 12；The bogey 13 includes motor 131, train of reduction gears 132, conical gear group 133, base
134th, bearing 135, support plate 136, culture dish 137, locating piece 138 and locating slot 139；The motor 131 passes through reducing gear
Wheel group 132 is connected with conical gear group 133；The top of the base 134 is rotated by bearing 135 and conical gear group 133
Connection；The support plate 136 is fixedly installed on the top of conical gear group 133；It is described to be distributed with everywhere in the top array for doing 136
Locating piece 138；The bottom of the culture dish 137 is provided with locating slot 139；The culture dish 137 is pacified by the locating slot 139 of bottom
It is in mating connection loaded on locating piece 138.
Wherein, the liquid discharge head 122 and liquid feeding first 125 are semicircular arc structure, and the structure of semicircular arc is in liquid discharge head
122 and liquid feeding it is first 125 disengaging culture dish when, nutrient solution can freely slide, and avoid being trapped on liquid discharge head 122 and liquid feeding first 125.
Wherein, the bottom of the liquid discharge head 122 and liquid feeding first 125 is equipped with drip plate 128, and drip plate 128 is in liquid feeding
Play a part of buffering, can avoid impacting the cell in culture dish in liquid feeding.
Wherein, the culture dish 137 is right against liquid discharge head 122 in the position on support plate 136 and liquid feeding first 125 is set, training
Ware 137 is supported under the rotation of bogey 13, turns to the bottom of liquid discharge head 122 and liquid feeding first 125, the He of liquid discharge head 122 successively
Liquid feeding first 125 does vertical movement up and down in the presence of lowering or hoisting gear 11, so as to realize discharge opeing and change liquid.
Wherein, the observation window 15 is right against culture dish 137 on horizontal level and set, and can be observed through observation window 15
The information such as the state of nutrient solution in culture dish 137, the color by observing nutrient solution can substantially judge cultivation stage and culture
Whether liquid goes bad.
Wherein, the discharging tube 121 and liquid-feeding tube 124 be located at the top of bearing 118 one section be helical form set it is soft
Pipe, one section positioned at the bottom of bearing 118 is plastic rigid pipe, and when bearing 118 moves up and down, the flexible pipe that helical form is set is stretched
Contracting, keep the normal connection of discharging tube 121 and liquid-feeding tube 124 and liquid discharge head 122 and liquid feeding first 125.
Wherein, the 7 S-type structure of heating wire is wound on the inwall of culturing room 6, and heating wire 7 is uniformly layed in
On the inwall of culturing room 6 so that homogeneous heating, gradient of temperature are steady.
Wherein, the charging valve 126 is electric-controlled type valve, and in liquid feeding, PLC 16 controls charging valve 26 to open,
Electronic flowmeter 127 monitors flow, and communicates information to PLC 16, and when completing liquid feeding, the control of PLC 16 adds
Liquid valve 126 is closed.
The specifically used mode of the present embodiment and effect：
In the use of the present invention, the nutrient solution changed and the nutrient solution newly added are temporarily stored in the waste liquid storage in fluid reservoir 5
In tank 9 and liquid feeding tank 10, lid 3 can be changed and added to waste tank 9 and liquid feeding tank 10 in opening, when needing to culture dish
137 when change liquid and addition, and the motion of bogey 13 is controlled by PLC 16, culture dish 137 is turned to and changes liquid dress
12 bottom is put, specifically, PLC 16 sends control signal, motor 131 passes through train of reduction gears 132 and circular cone
The rotation of support plate 136 is realized in the transmission of gear train 133, and array distribution has culture dish 137 everywhere on support plate 136, and support plate 136 is each
An angle of 90 degrees is rotated, culture dish 137 is turned to the bottom of discharging tube 121 and liquid-feeding tube 124, liquid is changed in the now control of lowering or hoisting gear 11
The motion of device 12, liquid and addition are changed by what liquid changing device 12 realized nutrient solution, specifically, lifting motor 113 passes through screw rod
117 with the transmission of block 116 of being spirally connected, and realize under the guide effect of guide pillar 114 and slide 115 and be vertically moved up or down up and down, by discharge opeing
First 122 and liquid feeding first 125 stretch into culture dish 137, positive displacement pump 123 is controlled by the culture in culture dish 137 by PLC 16
Liquid is extracted and kept in waste tank 9, and then PLC 16 controls charging valve 26 to open, the nutrient solution in liquid feeding tank 10
It is flowed into by liquid-feeding tube 124 in culture dish 137, electronic flowmeter 127 monitors flow, and communicates information to PLC
16, when completing liquid feeding, PLC 16 controls charging valve 126 to close, so as to realize the replacing of nutrient solution；The present invention is cultivating
It is equipped with heating wire 7 on the inwall of room 6, and is controlled by temperature sensor 8 by PLC 16 come monitoring temperature information
The work of heating wire 7 carrys out regulation culture temperature, and the heat-insulation layer 2 of rock wool material is provided with the inwall of casing 1, is advantageous to keep culture
Temperature it is constant, and reduce heat and scatter and disappear, reduce energy resource consumption.
Using technical scheme of the present invention, or those skilled in the art is under the inspiration of technical solution of the present invention, if
Similar technical scheme is counted out, and reaches above-mentioned technique effect, is to fall into protection scope of the present invention.
Claims (8)
- A kind of 1. medical experiment incubator of automatically replaceable nutrient solution, it is characterised in that：The medical experiment is used can automatically more Changing the incubator of nutrient solution includes casing, heat-insulation layer, upper lid, dividing plate, fluid reservoir, culturing room, heating wire, temperature sensor, gives up Liquid storage tank, liquid feeding tank, lowering or hoisting gear, column, fixed plate, lifting motor, guide pillar, slide, be spirally connected block, screw rod, bearing, changes liquid dress Put, discharging tube, liquid discharge head, positive displacement pump, liquid-feeding tube, liquid feeding head, charging valve, electronic flowmeter, drip plate, bogey, drive Motor, train of reduction gears, conical gear group, base, bearing, support plate, culture dish, locating piece, locating slot, chamber door, observation window and PLC；The madial wall of the casing is provided with heat-insulation layer, and the heat-insulation layer is heatproof rock wool material；The casing it is interior Portion is separated to form two chambers above and below fluid reservoir and culturing room by dividing plate；The waste tank and liquid feeding tank are located at fluid reservoir It is interior；The top of the waste tank and liquid feeding tank is provided with upper lid in casing；The temperature sensor is installed on the bottom of dividing plate； The outside of the culturing room by hinge on casing in being hinged with chamber door；The chamber door is provided with observation window；The PLC On the outer wall of casing；The lowering or hoisting gear is located in culturing room；The lowering or hoisting gear includes column, fixed plate, lifting electricity Machine, guide pillar, slide, the block that is spirally connected, screw rod and bearing；The fixed plate is fixedly installed in the top side of column；The lifting motor peace Top loaded on fixed plate；The clutch end of the lifting motor is connected with screw rod；The bottom of the fixed plate is in screw rod Both sides be provided with two at guide pillar；The slide is sheathed on guide pillar；The block that is spirally connected is sheathed on screw rod；Block and the cunning of being spirally connected Seat is integral type structure；The right-hand member of the slide is provided with the bearing of integral type；The liquid changing device is installed on bearing；It is described to change Liquid device includes discharging tube, liquid discharge head, positive displacement pump, liquid-feeding tube, liquid feeding head, charging valve, electronic flowmeter and drip plate；The row Liquid pipe and liquid-feeding tube are arranged on bearing；The bottom of the discharging tube and liquid-feeding tube is respectively equipped with liquid discharge head and liquid feeding head；Institute The top for stating discharging tube and liquid-feeding tube is connected with waste tank and liquid feeding tank respectively；The discharging tube is provided with positive displacement pump；Institute State liquid-feeding tube and be provided with charging valve and electronic flowmeter；The bogey is located at the bottom of liquid changing device；The bogey Including motor, train of reduction gears, conical gear group, base, bearing, support plate, culture dish, locating piece and locating slot；It is described Motor is connected by train of reduction gears with conical gear group；The top of the base is turned by bearing and conical gear group Dynamic connection；The support plate is fixedly installed on the top of conical gear group；It is described that locating piece everywhere is distributed with the top array done； The bottom of the culture dish is provided with locating slot；It is in mating connection that the culture dish by the locating slot of bottom is installed on locating piece.
- 2. the medical experiment as claimed in claim 1 incubator of automatically replaceable nutrient solution, it is characterised in that：The liquid discharge head It is semicircular arc structure with liquid feeding head.
- 3. the medical experiment as claimed in claim 1 or 2 incubator of automatically replaceable nutrient solution, it is characterised in that：The row The bottom of liquid head and liquid feeding head is equipped with drip plate.
- 4. the medical experiment as claimed in claim 1 incubator of automatically replaceable nutrient solution, it is characterised in that：The culture dish Liquid discharge head is right against in the position on support plate and liquid feeding head is set.
- 5. the incubator of the automatically replaceable nutrient solution of medical experiment as claimed in claim 1, it is characterised in that：The observation window Culture dish setting is right against on horizontal level.
- 6. the incubator of the automatically replaceable nutrient solution of medical experiment as claimed in claim 1, it is characterised in that：The discharging tube It is flexible pipe that helical form is set to be located at one section of holder top with liquid-feeding tube, and one section positioned at rest base is plastic rigid pipe.
- 7. the medical experiment as claimed in claim 1 incubator of automatically replaceable nutrient solution, it is characterised in that：The heating wire S-type structure is wound on the inwall of culturing room.
- 8. the medical experiment as claimed in claim 1 incubator of automatically replaceable nutrient solution, it is characterised in that：The charging valve For electric-controlled type valve.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710993956.XA CN107686812A (en) | 2017-10-23 | 2017-10-23 | A kind of incubator of the automatically replaceable nutrient solution of medical experiment |
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| CN201710993956.XA CN107686812A (en) | 2017-10-23 | 2017-10-23 | A kind of incubator of the automatically replaceable nutrient solution of medical experiment |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108267395A (en) * | 2018-04-13 | 2018-07-10 | 佛山市恩正生物医药科技有限公司 | A kind of stream type cell analyzer |
| CN108441421A (en) * | 2018-04-02 | 2018-08-24 | 郭庆 | A kind of magnetotactic bacteria culture instrument |
| CN108913602A (en) * | 2018-10-10 | 2018-11-30 | 山西正康生物科技有限公司 | A kind of cell cultivation equipment |
| CN109554287A (en) * | 2018-12-21 | 2019-04-02 | 华中科技大学同济医学院附属协和医院 | A kind of medical experiment culture apparatus |
| CN110004053A (en) * | 2019-05-14 | 2019-07-12 | 内江师范学院 | A kind of semi-automatic controllable temperature culture solution racking machine |
| CN110090586A (en) * | 2019-04-30 | 2019-08-06 | 黄国宁 | A kind of culture dish automatic liquid matching equipment |
| CN111518667A (en) * | 2020-04-30 | 2020-08-11 | 戴知 | Liquid medium replacing device with low potential pollution |
| CN111996115A (en) * | 2020-07-15 | 2020-11-27 | 吴晨凯 | Living cell microscopic imaging equipment |
| CN113817607A (en) * | 2021-10-27 | 2021-12-21 | 谢贤河 | Sealed cell culture box capable of timely replacing water and culture solution |
| CN114085773A (en) * | 2021-11-26 | 2022-02-25 | 李剑平 | Automatic stem cell culture equipment |
| CN114231413A (en) * | 2021-12-16 | 2022-03-25 | 潘烨 | A isolated culture equipment that is used for hematology branch of academic or vocational study marrow mesenchymal stem cell |
-
2017
- 2017-10-23 CN CN201710993956.XA patent/CN107686812A/en not_active Withdrawn
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441421A (en) * | 2018-04-02 | 2018-08-24 | 郭庆 | A kind of magnetotactic bacteria culture instrument |
| CN108267395A (en) * | 2018-04-13 | 2018-07-10 | 佛山市恩正生物医药科技有限公司 | A kind of stream type cell analyzer |
| CN108913602A (en) * | 2018-10-10 | 2018-11-30 | 山西正康生物科技有限公司 | A kind of cell cultivation equipment |
| CN109554287A (en) * | 2018-12-21 | 2019-04-02 | 华中科技大学同济医学院附属协和医院 | A kind of medical experiment culture apparatus |
| CN110090586A (en) * | 2019-04-30 | 2019-08-06 | 黄国宁 | A kind of culture dish automatic liquid matching equipment |
| CN110004053A (en) * | 2019-05-14 | 2019-07-12 | 内江师范学院 | A kind of semi-automatic controllable temperature culture solution racking machine |
| CN111518667A (en) * | 2020-04-30 | 2020-08-11 | 戴知 | Liquid medium replacing device with low potential pollution |
| CN111518667B (en) * | 2020-04-30 | 2021-05-04 | 苏州乐米凡电气科技有限公司 | Liquid medium replacing device with low potential pollution |
| CN111996115A (en) * | 2020-07-15 | 2020-11-27 | 吴晨凯 | Living cell microscopic imaging equipment |
| CN113817607A (en) * | 2021-10-27 | 2021-12-21 | 谢贤河 | Sealed cell culture box capable of timely replacing water and culture solution |
| CN113817607B (en) * | 2021-10-27 | 2024-03-22 | 山东大华凯特生物集团有限公司 | Sealed cell incubator capable of timely replacing water and culture solution |
| CN114085773A (en) * | 2021-11-26 | 2022-02-25 | 李剑平 | Automatic stem cell culture equipment |
| CN114231413A (en) * | 2021-12-16 | 2022-03-25 | 潘烨 | A isolated culture equipment that is used for hematology branch of academic or vocational study marrow mesenchymal stem cell |
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