CN105910962B - The vertical diffusion test device of full-automatic transdermal test in vitro - Google Patents
The vertical diffusion test device of full-automatic transdermal test in vitro Download PDFInfo
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- CN105910962B CN105910962B CN201610350816.6A CN201610350816A CN105910962B CN 105910962 B CN105910962 B CN 105910962B CN 201610350816 A CN201610350816 A CN 201610350816A CN 105910962 B CN105910962 B CN 105910962B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
- G01N13/04—Investigating osmotic effects
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
- G01N2013/003—Diffusion; diffusivity between liquids
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a kind of full-automatic vertical diffusion test devices of transdermal test in vitro, it includes four-way rotation valve, heat block and controller, it is connected with supplement solution bottle on four-way rotation valve, vertical diffusion batteries, multichannel injection pump group and sample divider are simultaneously respectively equipped with connection flexible pipe, supplement solution bottle has thermostat, vertical diffusion cell in vertical diffusion batteries is each provided in the heating tank on heat block, heat block is equipped with stepper motor, temperature sensor, heating plate and magnetic stirrer, thermostat, four-way rotates valve, stepper motor, temperature sensor, heating plate, magnetic stirrer, multichannel injection pump group, electric connection line is respectively equipped between sample divider and controller.The present invention has being capable of automatic time sampling, supplement medium, exhaust, the advantages of collecting sample, detergent line, completing full-automatic transdermal test in vitro vertical diffusion test.
Description
Technical field
The invention belongs to percutaneous drug administration preparation In vitro penetration technical field of test equipment, more particularly to a kind of full-automatic
The vertical diffusion test device of transdermal test in vitro.
Background technology
The main method that the experiment of percutaneous drug administration preparation In vitro penetration uses is diffusion cell method.This method can reflect medicine
Object can screen Transdermal absorption drug preparation prescription by the speed and degree of living animal Cutaneous permeation, be that the current world is recognized
Can Drug Percutaneous Absorption experiment standard detecting method.Diffusion cell applied to In vitro penetration experiment mainly has 2 kinds, list
Room diffusion cell and dual chamber diffusion cell.Single chamber diffusion cell is mainly Franz diffusion cells and the Franz diffusion cells of improvement.Franz expands
Scattered pond is a kind of vertical diffusion cell, and there are two parts to form, acceptance pool and supply pool, and skin can be clamped between two half Room of glass
Sample, the supply chamber on upper layer is usually added with drug with carrier, and receiving chamber is generally equipped with accepting medium.When being tested
When, heated constant temperature is kept for 32 DEG C or 37 DEG C while acceptance pool and supply pool stir respectively.
The no automatic sampling of percutaneous dispersion test instrument of domestic production at present and the function of being automatically replenished medium, need to lean on
Artificial treatment.General penetrating absorption uses 6 groups of diffusion cells, and sampling number is up to more than ten times, and test period is up to tens hours, sample
Quantity is even more up to tens.Therefore, manual sampling, which exists, expends that energy is big, the uncontrollable defect of human error, especially
It is that there is take the problems such as liquid fluid infusion is inconvenient, exhaust is difficult because sampling due to pipe diameter is small for vertical diffusion cell.
Invention content
It provides one kind the purpose of the present invention is overcoming the deficiencies in the prior art and changing traditional vertical expansion
Under the premise of the test method for dissipating pond, can automatic time sampling, be automatically replenished medium, automatic exhaust, automatic collection sample, automatically
The vertical diffusion test device of full-automatic transdermal test in vitro of detergent line.
The object of the present invention is achieved like this：It includes that four-way rotation valve, heat block and controller, four-way rotate on valve
Be connected with supplement solution bottle, vertical diffusion batteries, multichannel injection pump group and sample divider, four-way rotate valve and supplement solution bottle it
Between be equipped with replenisher connection flexible pipe；Four-way, which rotates, is equipped with sampling connection flexible pipe between valve and vertical diffusion batteries；Four-way rotates valve
Syringe pump connection flexible pipe is equipped between multichannel injection pump group；Four-way, which rotates, is equipped with collector company between valve and sample divider
Connect hose, the supplement solution bottle has a thermostat, the vertical diffusion batteries at least by a vertical diffusion batteries at,
All vertical diffusion cells are each provided in the heating tank on heat block, and heat block is equipped with stepper motor, temperature sensor, heating
Piece and magnetic stirrer, the thermostat, four-way rotation valve, stepper motor, temperature sensor, heating plate, magnetic stirrer,
It is respectively equipped with electric connection line between multichannel injection pump group, sample divider and controller.
The vertical diffusion cell includes vertical supply pool, vertical acceptance pool and diffusion cell sample tap, each vertical diffusion
The diffusion cell sample tap that pond is equipped with is both connected in sampling connection flexible pipe.
The multichannel injection pump group is made of multichannel injection pump identical with vertical diffusion cell quantity, each mostly logical
Road syringe pump is both connected in syringe pump connection flexible pipe, is respectively equipped with and is electrically connected between each multichannel injection pump and controller
Line.
The sample divider includes the sample bottle group that sampling probe group and sampling probe group have, the sampling probe group
It is made of sampling probe identical with vertical diffusion cell quantity, all sampling probes are commonly connected in collector connection flexible pipe, institute
Some sampling probes are each provided in sampling probe fixed plate, and sampling probe fixed plate is located on horizontal stand, and horizontal stand and sampling probe are solid
Mobile screw and horizontal step motor are equipped between fixed board, horizontal stand is located on vertical support frame, and vertical support frame is located at horizontal straight
On line guide rail, vertical support frame stepper motor is equipped between vertical support frame and horizontal linear guide rail；The sample bottle group is at least wrapped
Two rows of sample bottle single groups are included, sample bottle single group is often arranged and is made of sample bottle identical with sampling probe quantity, all sample bottles are equal
Be located on sample tray, sample tray be equipped with waste liquid outlet, the vertical support frame stepper motor, horizontal step motor with
Electric connection line is respectively equipped between controller.
Due to the adoption of the above technical scheme, a kind of full-automatic vertical diffusion test device tool of transdermal test in vitro provided by the invention
It has the following advantages：Can automatic time sampling, be automatically replenished medium, automatic exhaust, automatic collection sample, automatic detergent line,
Complete the vertical diffusion test of full-automatic transdermal test in vitro.
Description of the drawings
Structural schematic diagram when Fig. 1 is vertical diffusion cell there are one the present invention is set.
In figure：1. vertical supply pool, 2. vertical acceptance pools, 3. stepper motors, 4. heating plates, 5. electromagnetic agitations
Device, 6. temperature sensors, 7. heat blocks, 8. diffusion cell sample taps, 9. thermostats, 10. supplement solution bottles, 11. four-ways turn
Dynamic valve, 12. multichannel injection pumps, 13. vertical support frame stepper motors, 14. vertical support frames, 15. horizontal stands, 16. movements
Screw rod and horizontal step motor, 17. collector connection flexible pipes, 18. sampling probes, 19. sampling probe fixed plates, 20. sampling bottles.
21. sample tray, 22. waste liquid outlets, 23, replenisher connection flexible pipe, 24, sampling connection flexible pipe, 25, syringe pump connection it is soft
Pipe.
Specific implementation mode
As shown in Figure 1, the vertical diffusion test device of full-automatic transdermal test in vitro provided by the invention include four-way rotation valve 11,
Heat block 7 and controller, four-way rotation valve 11 on be connected with supplement solution bottle 10, vertical diffusion batteries, multichannel injection pump group and
Sample divider, four-way, which rotates, is equipped with replenisher connection flexible pipe 23 between valve 11 and supplement solution bottle 10；Four-way rotates valve 11 and stands
Sampling connection flexible pipe 24 is equipped between formula diffusion batteries；Four-way, which rotates, is equipped with syringe pump company between valve 11 and multichannel injection pump group
Connect hose 25；Four-way rotates and is equipped with collector connection flexible pipe 17 between valve 11 and sample divider, under the supplement solution bottle 10
Equipped with thermostat 9, the vertical diffusion batteries are at least by a vertical diffusion batteries at all vertical diffusion cells are each provided at
In heating tank on heat block 7, heat block 7 is equipped with stepper motor 3, temperature sensor 6, heating plate 4 and magnetic stirrer 5,
The thermostat 9, four-way rotation valve 11, stepper motor 3, temperature sensor 6, heating plate 4, magnetic stirrer 5, multichannel note
It penetrates between pump group, sample divider and controller and is respectively equipped with electric connection line.
The vertical diffusion cell includes vertical supply pool 1, vertical acceptance pool 2 and diffusion cell sample tap 8, each vertical expansion
The diffusion cell sample tap 8 that scattered pond is equipped with is both connected in sampling connection flexible pipe 24.
The multichannel injection pump group is made of multichannel injection pump identical with vertical diffusion cell quantity 12, Mei Geduo
Channel injection pump 12 is both connected in syringe pump connection flexible pipe 25, is respectively equipped between each multichannel injection pump 12 and controller
Electric connection line.
The sample divider includes the sample bottle group that sampling probe group and sampling probe group have, the sampling probe group
It is made of sampling probe identical with vertical diffusion cell quantity 18, all sampling probes 18 are commonly connected to collector connection flexible pipe 17
On, all sampling probes 18 are each provided in sampling probe fixed plate 19, and sampling probe fixed plate 19 is located on horizontal stand 15, level branch
Mobile screw and horizontal step motor 16 are equipped between frame 15 and sampling probe fixed plate 19, horizontal stand 15 is located at vertical support frame 14
On, vertical support frame 14 is located on horizontal linear guide rail, and vertical support frame stepping is equipped between vertical support frame 14 and horizontal linear guide rail
Motor 13；The sample bottle group includes at least two rows of sample bottle single groups, often arrange sample bottle single group by with 18 quantity phase of sampling probe
Same sample bottle 20 forms, and all sample bottles 20 are each provided on sample tray 21, and sample tray 21 is equipped with waste liquid outlet
22, it is respectively equipped with electric connection line between the vertical support frame stepper motor 13, horizontal step motor 16 and controller.
It is first that vertical diffusion cell adds replenisher by controller, then from vertical diffusion cell to taking when specifically used
Sample bottle 20 samples, the finally detergent line between vertical diffusion cell and sampling probe 18.
Work step is as follows：The first step is that vertical diffusion cell adds replenisher by controller.Controller rotary four-way turns
Dynamic valve 11 connects replenisher connection flexible pipe 23 and sampling connection flexible pipe 24 between multichannel injection pump 12 and supplement solution bottle, mostly logical
Road syringe pump 12 simultaneously first suck 2mL replenishers, controller rotary four-way rotate valve 11 connect multichannel injection pump 12 with it is vertical
Sampling connection flexible pipe 24 between the diffusion cell sample tap 8 of diffusion cell and syringe pump connection flexible pipe 25, multichannel injection pump 12 push away
Enter in replenisher to the diffusion cell sample tap 8 of vertical diffusion cell.Then controller control multichannel injection pump 12 passes through replenisher
Connection flexible pipe 23 and sampling connection flexible pipe 24 suck 2mL replenishers again from supplement solution bottle 10, to the diffusion cell of vertical diffusion cell
8 inner push-in replenisher of sample tap, when being pushed into half, stepper motor 3 starts to rotate heat block 7, makes vertical diffusion cell sample tap 8
It upwardly-directed rotates, i.e., the air of the vertical diffusion cell first half is gradually moved to diffusion cell sample tap 8, while being pushed further into replenisher
Replenisher is filled while air is discharged in vertical diffusion cell.
Second step is sampling.Controller rotation four-way rotation valve 11 connect the diffusion cell sample tap 8 of vertical diffusion cell with it is more
Syringe pump connection flexible pipe 25 between channel injection pump 12 and sampling connection flexible pipe 24, multichannel injection pump 12 is from vertical diffusion cell
Diffusion cell sample tap 8 suck 1mL or so diffusion liquid, controller be rotated further by four-way rotation valve 11 connect multichannel injection pump 12 with
Syringe pump connection flexible pipe 25 between sampling probe 18 and collector connection flexible pipe 17, while controller controls vertical support frame 14 in water
It is moved on flat line guide rail and the sampling in the sampling probe fixed plate 19 and sampling probe fixed plate 19 being located on horizontal stand 15
Needle 18 moves on to 21 first row sampling bottle of sample tray, 20 top, and controller rotary screw makes sampling probe fixed plate 19 move down, samples
Needle 18 punctures the lid of sampling bottle 20 and enters in bottle, and controller manipulates multichannel injection pump 12 and is pushed into diffusion liquid into sampling bottle 20, most
Controller control vertical support frame stepper motor 13 again moves on to sampling probe 18 in the linear guide movement in the rectangle of sample tray 21 afterwards
Above slot, terminate sampling operation.
Third step is detergent line.Four-way, which is rotated, by controller rotates the connection multichannel injection pump 12 of valve 11 and replenisher
Syringe pump connection flexible pipe 25 between bottle 10 and replenisher connection flexible pipe 23, the sucking out of supplement solution bottle 10 of multichannel injection pump 12
After 0.5mL or so replenishers, then by controller rotate four-way rotate valve 11 connect multichannel injection pump 12 and sampling probe 18 it
Between syringe pump connection flexible pipe 25 and collector connection flexible pipe 17, the replenisher sucked out of supplement solution bottle 10 is discharged to sample
Pallet 21 is discharged from the waste liquid outlet 22 of sample tray 21, this step repeats 2~3 times.
The first step of reruning after third the end of the step add replenisher, magnitude of recruitment is equal with sampling amount, repeatedly above step into
Row repeatedly sampling.
Claims (4)
1. a kind of vertical diffusion test device of full-automatic transdermal test in vitro, it is characterised in that：It include four-way rotation valve, heat block and
Controller, four-way rotate and are connected with supplement solution bottle, vertical diffusion batteries, multichannel injection pump group and sample divider on valve, and four
It is equipped with replenisher connection flexible pipe between logical rotation valve and supplement solution bottle；Four-way, which rotates, is equipped with sampling between valve and vertical diffusion batteries
Connection flexible pipe；Four-way, which rotates, is equipped with syringe pump connection flexible pipe between valve and multichannel injection pump group；Four-way rotates valve and is received with sample
Collector connection flexible pipe is equipped between storage, the supplement solution bottle has thermostat, and the vertical diffusion batteries are at least
By a vertical diffusion batteries at all vertical diffusion cells are each provided in the heating tank on heat block, and heat block is equipped with step
Stepper motor, temperature sensor, heating plate and magnetic stirrer, the thermostat, four-way rotation valve, stepper motor, temperature pass
It is respectively equipped with electric connection line between sensor, heating plate, magnetic stirrer, multichannel injection pump group, sample divider and controller.
2. the vertical diffusion test device of full-automatic transdermal test in vitro according to claim 1, it is characterised in that：Described is vertical
Diffusion cell includes vertical supply pool, vertical acceptance pool and diffusion cell sample tap, the diffusion cell sampling that each vertical diffusion cell is equipped with
Mouth is both connected in sampling connection flexible pipe.
3. the vertical diffusion test device of full-automatic transdermal test in vitro according to claim 1, it is characterised in that：Described is mostly logical
Injection pump group in road is made of multichannel injection pump identical with vertical diffusion cell quantity, and each multichannel injection pump is both connected to note
It penetrates in pump connection flexible pipe, electric connection line is respectively equipped between each multichannel injection pump and controller.
4. the vertical diffusion test device of full-automatic transdermal test in vitro according to claim 1, it is characterised in that：The sample
Collector includes the sample bottle group that sampling probe group and sampling probe group have, the sampling probe group by with vertical diffusion cell quantity
Identical sampling probe composition, all sampling probes are commonly connected in collector connection flexible pipe, and all sampling probes, which are each provided at, to be taken
In sample needle fixed plate, sampling probe fixed plate is located on horizontal stand, and mobile spiral shell is equipped between horizontal stand and sampling probe fixed plate
Bar and horizontal step motor, horizontal stand are located on vertical support frame, and vertical support frame is located on horizontal linear guide rail, vertical support frame and
Vertical support frame stepper motor is equipped between horizontal linear guide rail；The sample bottle group includes at least two rows of sample bottle single groups, often
Row's sample bottle single group is made of sample bottle identical with sampling probe quantity, and all sample bottles are each provided on sample tray, sample
Pallet is equipped with waste liquid outlet, vertical support frame stepper motor, mobile screw and the horizontal step motor and controller it
Between be respectively equipped with electric connection line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610350816.6A CN105910962B (en) | 2016-05-25 | 2016-05-25 | The vertical diffusion test device of full-automatic transdermal test in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610350816.6A CN105910962B (en) | 2016-05-25 | 2016-05-25 | The vertical diffusion test device of full-automatic transdermal test in vitro |
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| Publication Number | Publication Date |
|---|---|
| CN105910962A CN105910962A (en) | 2016-08-31 |
| CN105910962B true CN105910962B (en) | 2018-08-14 |
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| CN201610350816.6A Expired - Fee Related CN105910962B (en) | 2016-05-25 | 2016-05-25 | The vertical diffusion test device of full-automatic transdermal test in vitro |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10201802032TA (en) * | 2018-03-12 | 2019-10-30 | Skinetrate Pte Ltd | Apparatus for determination of diffusion or permeation through a specimen, method of forming the same and method for controlling the same |
| CN112213237A (en) * | 2020-09-01 | 2021-01-12 | 中国辐射防护研究院 | Device and method for researching diffusion of radioactive nuclide in mudstone core |
| CN112945801B (en) * | 2021-02-01 | 2024-02-09 | 南京海关工业产品检测中心 | Transdermal diffusion cell for simulating gargling process and transdermal diffusion experimental instrument |
| CN113376062A (en) * | 2021-07-06 | 2021-09-10 | 深圳市华溶分析仪器有限公司 | Transdermal experimental device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201063030Y (en) * | 2007-06-15 | 2008-05-21 | 阮卫明 | Experimental device for testing penetration of skin by medicament |
| CN103115852A (en) * | 2013-01-15 | 2013-05-22 | 禄根仪器(镇江)有限公司 | Transdermal diffusion apparatus |
| CN205991937U (en) * | 2016-05-25 | 2017-03-01 | 延边大学 | Full-automatic transdermal test in vitro vertical diffusion test device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0755686A (en) * | 1993-08-10 | 1995-03-03 | Sumitomo Chem Co Ltd | Method and device for evaluating physical property of film |
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2016
- 2016-05-25 CN CN201610350816.6A patent/CN105910962B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201063030Y (en) * | 2007-06-15 | 2008-05-21 | 阮卫明 | Experimental device for testing penetration of skin by medicament |
| CN103115852A (en) * | 2013-01-15 | 2013-05-22 | 禄根仪器(镇江)有限公司 | Transdermal diffusion apparatus |
| CN205991937U (en) * | 2016-05-25 | 2017-03-01 | 延边大学 | Full-automatic transdermal test in vitro vertical diffusion test device |
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| CN105910962A (en) | 2016-08-31 |
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Granted publication date: 20180814 Termination date: 20190525 |