CN112945801B - Transdermal diffusion cell for simulating gargling process and transdermal diffusion experimental instrument - Google Patents
Transdermal diffusion cell for simulating gargling process and transdermal diffusion experimental instrument Download PDFInfo
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- CN112945801B CN112945801B CN202110137054.2A CN202110137054A CN112945801B CN 112945801 B CN112945801 B CN 112945801B CN 202110137054 A CN202110137054 A CN 202110137054A CN 112945801 B CN112945801 B CN 112945801B
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- 238000009792 diffusion process Methods 0.000 title claims abstract description 79
- 238000000034 method Methods 0.000 title claims abstract description 16
- 230000005540 biological transmission Effects 0.000 claims abstract description 45
- 238000007664 blowing Methods 0.000 claims abstract description 33
- 238000012377 drug delivery Methods 0.000 claims abstract description 27
- 238000002474 experimental method Methods 0.000 claims abstract description 22
- 239000003814 drug Substances 0.000 claims abstract description 20
- 230000000149 penetrating effect Effects 0.000 claims abstract description 5
- 238000005070 sampling Methods 0.000 claims description 56
- 210000002200 mouth mucosa Anatomy 0.000 claims description 28
- 230000033001 locomotion Effects 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 9
- 230000001681 protective effect Effects 0.000 claims description 6
- 239000000243 solution Substances 0.000 description 18
- 210000000214 mouth Anatomy 0.000 description 16
- 238000001647 drug administration Methods 0.000 description 7
- 230000003028 elevating effect Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 210000004400 mucous membrane Anatomy 0.000 description 4
- 238000010276 construction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000001097 facial muscle Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
- G01N13/04—Investigating osmotic effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
- G01N2013/003—Diffusion; diffusivity between liquids
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dental Tools And Instruments Or Auxiliary Dental Instruments (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The invention discloses a transdermal diffusion cell and a transdermal diffusion experimental instrument for a gargling process, and belongs to the technical field of transdermal experiments. The transdermal diffusion cell comprises a receiving cell and a drug delivery cell, wherein the upper end of the drug delivery cell is provided with an air blowing device, and the air blowing device comprises a base, a first power source, a first transmission unit, a second transmission unit, a lifting table, an air inlet unit and a control module; the air inlet unit comprises a second power source and an air inlet rod, an air inlet through hole penetrating axially is formed in the air inlet rod, an air basket is arranged at one end of the air inlet rod, and the air inlet through hole is communicated with the air basket; the other end is connected with the output end of the second power source through the gas receiving component; the air inlet rod penetrates through the base, so that the air basket is located in the medicine feeding tank, an air inlet interface used for being connected with an external air source is arranged on the air receiving component, and when the external air source supplies air, air can enter the air basket through the air inlet through hole and is blown into the medicine feeding tank through the air basket. The invention can make the environment in the diffusion pool more approximate to the oral environment in gargling, so that the transdermal experimental result is more accurate.
Description
Technical Field
The invention belongs to the technical field of transdermal experiments, and particularly relates to a transdermal diffusion cell and a transdermal diffusion experiment instrument for simulating a gargling process.
Background
The transdermal diffusion device is used for testing the skin penetration rate of medicines, the skin to be tested is clamped in a diffusion cell, the diffusion cell is placed in the transdermal diffusion device, the medicine to be tested on the upper side of the skin penetrates through the skin of the diffusion cell in a heating mode of the diffusion cell, the medicine to be tested is soaked into physiological saline on the lower side of the skin, the concentration in a detection solvent is periodically extracted, the amount of the medicine penetrating through the skin is analyzed, experimental data recording is carried out, and finally the average data are obtained through multiple evaluations.
At present, when transdermal diffusion experiments are carried out, franz diffusion cells are often adopted, the structure of the diffusion cells of the type is shown in fig. 1, the diffusion cells comprise a receiving cell and a drug administration cell, when diffusion experiments are carried out, the drug administration cell is buckled on the receiving cell and communicated with the receiving cell, sampling holes are formed in the receiving cell, sampling is convenient, the upper part of the drug administration cell is communicated with the atmosphere, and a membrane or skin for experiments is clamped between the receiving cell and the drug administration cell and then clamped by a pipe clamp. However, in the transdermal diffusion test, there is a test to simulate the transdermal diffusion process during rinsing, and it is known from life experience that during rinsing, the rinse is continuously rolled in the mouth, the rinse has a certain impact force on the mucous membrane of the mouth, and because the transdermal diffusion test is simulated in vitro, more factors need to be considered when simulating the transdermal diffusion of the mucous membrane of the mouth during rinsing in vitro, for example, how to ensure that water flow can stably impact the mucous membrane, prevent the mucous membrane from being damaged, etc. Experimental procedures find that when the existing diffusion cell is used for simulating the transdermal diffusion process of the oral mucosa during rinsing, a better experimental effect is difficult to achieve.
Disclosure of Invention
Technical problems: aiming at the problem that the existing transdermal diffusion cell is difficult to effectively simulate the transdermal diffusion process of the oral mucosa in the process of rinsing, the invention provides the transdermal diffusion cell for simulating the rinsing process, and the oral environment can be more closely contacted by the transdermal diffusion cell in the process of rinsing, so that the transdermal diffusion process of the oral mucosa in the process of rinsing can be more accurately simulated; further, a transdermal diffusion experiment instrument capable of more accurately simulating the transdermal diffusion process of oral mucosa in the mouth rinsing process is provided.
The technical scheme is as follows: the invention relates to a transdermal diffusion cell for simulating a gargling process, which is used for oral mucosa transdermal diffusion experiments and comprises a receiving cell and a drug delivery cell, wherein the drug delivery cell is buckled at the upper end of the receiving cell and is communicated with the receiving cell, and oral mucosa is arranged between the drug delivery cell and the receiving cell, and the transdermal diffusion cell is characterized in that the upper end of the drug delivery cell is provided with a blowing device, and the blowing device comprises:
the base is buckled on the drug delivery tank;
the first power source is arranged on the base;
the first transmission unit is connected with the output end of the first power source;
the second transmission unit is arranged on the base and connected with the first transmission unit, and the power of the first power source is transmitted to the second transmission unit through the first transmission unit;
the lifting platform is arranged on the second transmission unit, and the first power source provides power for the up-and-down movement of the lifting platform;
the air inlet unit comprises a second power source and an air inlet rod, and the second power source is arranged on the lifting platform; an air inlet through hole penetrating axially is formed in the air inlet rod, an air basket is arranged at one end of the air inlet rod, and the air inlet through hole is communicated with the air basket; the other end is connected with the output end of the second power source through the gas receiving component; the air inlet rod penetrates through the base, so that the air basket is positioned in the medicine feeding tank, an air inlet interface for connecting an external air source is arranged on the air receiving component, and when the external air source supplies air, air can enter the air basket through the air inlet through hole and is blown into the medicine feeding tank through the air basket;
and the control module is connected with the first power source and the second power source.
Further, the air receiving part comprises an outer shell and a connecting sleeve rotatably arranged in the outer shell, one end of the connecting sleeve is connected with the air inlet rod, and the other end of the connecting sleeve is connected with the output end of the second power source; a closed air chamber is formed between the connecting sleeve and the outer shell, and a plurality of air holes are formed in the connecting sleeve; the air inlet interface is arranged on the outer shell and is communicated with the air chamber, and air can enter the air inlet through hole through the air hole.
Further, the second transmission unit comprises a driving gear arranged on the base, and at least two driven gears which are arranged around the periphery of the driving gear and meshed with the driving gear, a screw rod is arranged on the driven gears, the lifting table is arranged on the screw rod through a screw rod nut, and the driven gears drive the screw rod to rotate so as to drive the lifting table to move up and down.
Further, a first through hole is formed in the base, a first rotating shaft is formed in the lower end face of the driving gear, a first bearing is sleeved on the first rotating shaft, and the first rotating shaft is arranged in the first through hole through the first bearing.
Further, a second through hole is formed in the center of the driving gear, the second through hole penetrates through the first rotating shaft, and the air inlet rod penetrates through the driving gear from the second through hole.
Further, the flow rate of outward blowing of the air basket is constant, the air basket rotates at a constant speed, the distance between the air basket and the oral mucosa is H cm, the upward or downward movement speed of the air basket is V cm/s, and V=kh and k is 0.3-0.96 in numerical value.
Further, the first transmission unit comprises a driving wheel arranged at the output end of the second power source and a driven wheel arranged on the driving gear, the driving wheel and the driven wheel are transmitted through a transmission belt, a third through hole is formed in the center of the driven wheel, and the air inlet rod penetrates through the third through hole.
Further, the stirring device further comprises a protective shell arranged on the base, and the first transmission unit and the second transmission unit are both positioned inside the protective shell.
Further, be provided with first sampling tube and first back flow on the pond of dosing, first sampling tube one end and pond intercommunication of dosing, the other end is opened there is first sampling port, is provided with first appearance chamber on the first sampling tube, and the one end of first back flow communicates with the pond of dosing, and the other end communicates with first appearance chamber.
Further, a second sampling tube is arranged on the receiving pool, one end of the second sampling tube is communicated with the receiving pool, a second sampling port is formed in the other end of the second sampling tube, and a second containing cavity is formed in the second sampling tube.
Further, the receiving pool up end is provided with a plurality of annular grooves, and the lower terminal surface of dosing pool corresponds to be provided with a plurality of annular bulge, when dosing pool detains on the receiving pool, annular bulge embedding is in corresponding annular groove.
Further, a pressure detection sensor is arranged at the lower end of the drug delivery tank, and the pressure detection sensor is connected with the control module.
The transdermal diffusion instrument for simulating the gargling process comprises the transdermal diffusion cell.
The beneficial effects are that: compared with the prior art, the invention has the following advantages:
(1) Compared with the prior art, the diffusion tank is provided with the stirring device, the base, the first power source, the first transmission unit, the second transmission unit, the lifting table, the blowing unit and the control module at the upper end of the administration tank, and the administration tank can be blown through the rotation and the up-and-down movement of the blowing unit, so that when oral mucosa transdermal diffusion experiments are carried out, the solution in the administration tank is blown, the environment of the solution in the administration tank is more similar to the environment in an oral cavity when mouth is rinsed, and the results of the oral mucosa transdermal diffusion experiments are more accurate.
(2) By utilizing the air receiving component, air can be introduced into the air basket from the air inlet rod in the rotating process of the air blowing unit, so that the air moves along with the movement of the air inlet unit, and the air inlet unit is more compact in structure.
(3) The second transmission unit comprises a driving gear and at least two driven gears meshed with the driving gear, and the driven gears are provided with screw rods, so that the lifting table can be driven to move up and down through the at least two screw rods, the stability of the up-and-down movement of the air blowing unit is further improved, the air blowing stability is further improved, and the environment in a medicine feeding pool is more similar to the environment in an oral cavity during mouth rinsing.
(4) The first sampling tube is arranged on the drug administration pond, so that the solution in the drug administration pond can be conveniently sampled and tested; furthermore, a first containing cavity is arranged on the first sampling tube, and the diffusion cell can be inclined when a proper sampling needle is lacked, so that sampling is facilitated; and a first return pipe is arranged, so that the solution can flow back during blowing, and overflow is avoided.
(5) The receiving pool is provided with the second sampling tube, and the second sampling tube is provided with the second containing cavity communicated with the second sampling tube, so that the solution in the receiving pool can be conveniently sampled and tested.
(6) The upper end surface of the receiving tank is provided with a plurality of annular grooves, the lower end surface of the feeding tank is correspondingly provided with a plurality of annular protrusions, and when the feeding tank is buckled on the receiving tank, the annular protrusions are embedded into the corresponding annular grooves, so that the mutual positioning of the feeding tank and the receiving tank is facilitated, the sealing is facilitated, and the leakage of a solution is avoided.
(7) The pressure detection sensor is arranged at the lower part of the drug delivery tank, so that the impact force born by the oral mucosa can be monitored in real time, and the oral mucosa is prevented from being impacted and damaged during transdermal diffusion experiments.
(8) The transdermal diffusion instrument comprises the diffusion cell provided by the invention, and can simulate the environment in the oral cavity during rinsing, so that the transdermal diffusion experiment of the oral mucosa during rinsing can be effectively performed, and the result has higher accuracy.
Drawings
FIG. 1 is a cross-sectional view of a prior art diffusion cell;
FIG. 2 is a cross-sectional view of a diffusion cell of the present invention (without a blowing device);
FIG. 3 is a front view of a diffusion cell of the present invention;
FIG. 4 is an exploded view of a diffusion cell of the present invention;
fig. 5 is a structural view (without a protective case) of the blowing device of the present invention;
fig. 6 is an exploded construction view of the blowing device of the present invention;
fig. 7 is an exploded construction view of the blowing device of the present invention;
FIG. 8 is a block diagram of an intake rod and basket of the present invention;
FIG. 9 is a block diagram of the air receiving member of the present invention;
FIG. 10 is a cross-sectional view of a receiving well of the present invention;
FIG. 11 is a cross-sectional view of a drug delivery reservoir of the present invention.
The drawings are as follows: 100. a receiving pool; 110. a second sampling tube; 111. a second sampling port; 120. a second cavity; 130. an annular groove;
200. a dosing tank; 210. a first sampling tube; 211. a first sampling port; 220. a first cavity; 230. an annular protrusion; 240. a first return pipe;
300. oral mucosa;
400. a magnetic stirrer;
500. an air blowing device;
510. a base; 511. a first through hole; 512. a mounting hole; 513. a protruding portion;
520. a first power source;
530. a first transmission unit; 531. a driving wheel; 532. a transmission belt; 533. driven wheel; 5331. a third through hole;
540. a second transmission unit; 541. a drive gear; 5411. a first rotating shaft; 5412. a first bearing; 5413. a second through hole; 542. a driven gear; 5421. a second rotating shaft; 5422. a second bearing; 543. a screw rod;
550. a lifting table; 551. a screw nut;
560. an air intake unit; 561. a second power source; 562. an air inlet rod; 5621. an air inlet through hole; 563. an air basket; 564. an air receiving component; 5641. an outer housing; 5642. connecting sleeves; 5643. a gas chamber; 5644. air holes; 5645. an air inlet interface; 565. a support frame;
570. a protective shell;
600. a pressure detection sensor.
Detailed Description
The invention is further described below in conjunction with the embodiments and the appended drawings, wherein the terms "first," "second," "third," etc. are used for ease of description only and are not to be construed as indicating or implying relative importance.
Referring to fig. 2, the transdermal diffusion cell of the present invention includes a receiving cell 100 and a delivery cell 200, the delivery cell 200 being fastened to the upper end of the receiving cell 100 and communicating with the receiving cell 100, and when performing a transdermal test, an oral mucosa 300 is disposed between the delivery cell 200 and the receiving cell 100, and a magnetic stirrer 400 is placed in the receiving cell 100. Compared with the prior art, the invention is different in that the upper end of the administration pond 200 is provided with the air blowing device 400 as shown in fig. 3 and 4, so that the solution in the administration pond 200 can be disturbed by blowing air into the administration pond 200, and a certain impact force is generated on the oral mucosa 300, so that the oral mucosa is closer to the environment in the oral cavity when the mouth is rinsed, and the result obtained by a transdermal diffusion experiment is more accurate. According to life experience, when the mouth is rinsed, the rinse in the mouth is disturbed by facial muscle movements, and the rinse is disturbed by air flow, so that the environment in the dosing tank 200 can be more approximate to the mouth environment by blowing air into the dosing tank 200. Specifically, as shown in fig. 5, 6 and 7, the blowing device 400 of the present invention includes: the device comprises a base 510, a first power source 520, a first transmission unit 530, a second transmission unit 540, a lifting platform 550, an air inlet unit 560 and a control module.
In the embodiment of the present invention, an annular protrusion 513 is provided on the lower side of the base 510, and the annular protrusion 513 can be embedded into the drug delivery tank 200, so that the blowing device 500 can be fixed on the drug delivery tank 200, and the solution in the drug delivery tank 200 can be prevented from overflowing from the upper end of the drug delivery tank 200 when the blowing device 500 is used for blowing the solution in the drug delivery tank 200.
The first power source 520 is disposed on the base 510, and in the embodiment of the present invention, the first power source 520 is a micro motor, and the micro motor is used as the power source to meet the size requirement because the size of the specific transdermal diffusion cell is relatively small.
The first transmission unit 530 is connected to an output end of the first power source 520, thereby transmitting power output from the first power source 520.
The second transmission unit 540 is disposed on the base 510 and connected to the first transmission unit 530, and the power of the first power source 520 is transmitted to the second transmission unit 540 through the first transmission unit 530. Specifically, in the embodiment of the present invention, the second transmission unit 540 includes a driving gear 541, at least two driven gears 542 surrounding the driving gear 541 and engaged with the driving gear 541, and a screw rod 543 is provided on the driven gears 542. Specifically, in the embodiment of the present invention, a first through hole 511 is formed in the base 510, a first rotating shaft 5411 is formed on the lower end surface of the driving gear 541, a first bearing 5412 is sleeved on the first rotating shaft 5411, and the first rotating shaft 5411 is disposed in the first through hole 511 through the first bearing 5412, so that the driving gear can rotate better. Also, in the embodiment of the present invention, in order to mount the driven gear 542 and enable good rotation of the driven gear 542, the mount hole 512 for mounting the driven gear 542 may be formed on the base 510, the second rotation shaft 5421 is formed on the lower end surface of the driven gear 542, the second rotation shaft 5421 is sleeved with the second bearing 5422, and the second rotation shaft 5421 is disposed in the corresponding mount hole 512 through the second bearing 5422.
The lifting platform 550 is arranged on the screw rod 543 through the screw rod nut 551, and the driven gear 542 rotates to drive the screw rod 543 to rotate, so that the lifting platform 550 can be driven to move up and down.
Specifically, the power output from the first power source 520 is transmitted to the second transmission unit 540 through the first transmission unit 530, and then the elevating platform 550 is driven to ascend or descend through the second transmission unit 540.
In the embodiment of the present invention, the first transmission unit 530 includes a driving wheel 531 provided at an output end of the first power source 520, a driven wheel 533 provided on the driving gear 541, and the driving wheel 531 and the driven wheel 533 are transmitted through a transmission belt 532. When the first power source 520 works, the driving wheel 531 is driven to rotate, then the driven wheel 533 is driven to rotate through the transmission belt 532, the driven wheel 533 drives the driving gear 541 to rotate, the driving gear 541 is meshed with the driven gear 542, so that the driven gear 542 is driven to rotate, when the driven gear 542 rotates, the screw rod 543 arranged on the driven gear 542 is driven to rotate, and when the screw rod 543 rotates, the rotation is converted into linear motion of the screw rod nut 551, so that the lifting table 550 is driven to move up and down.
In the embodiment of the invention, at least two driven gears 542 are provided, so that at least two screw rods 543 are provided, and all driven gears 542 are driven by one driving gear 541, so that all driven gears 542 rotate synchronously, so that all screw rods 543 still rotate synchronously, thereby ensuring the stability of the up-and-down movement process of the lifting platform 550 and further ensuring the stability of the blowing process.
For example, in one embodiment of the present invention, as shown in fig. 5 to 7, 4 driven gears 542 are uniformly provided along the outer circumference of the driving gear 541, so that the lifting table 550 is driven to move up and down by having 4 screw rods 543 synchronously rotated, so that the stability of the lifting table is better when the lifting table is left to move, and the stability of the lifting table when the blowing operation is performed is further ensured.
The air intake unit 560 includes a second power source 561 and an air intake rod 562, and the second power source 561 is provided on the elevating platform 550. As shown in fig. 8, an air inlet through hole 5621 penetrating in the axial direction is formed in the air inlet rod 562, an air basket 563 is arranged at one end of the air inlet rod 562, and the air inlet through hole 5621 is communicated with the air basket 563; the other end is connected with the output end of the second power source 561 through the gas receiving component 564; the air inlet rod 562 penetrates through the base 510, so that the air basket 563 is located in the medicine feeding tank 200, the air receiving part 564 is provided with an air inlet port 5645 for connecting an external air source, and when the external air source supplies air, air can enter the air basket 563 through the air inlet through hole 5621 and blow into the medicine feeding tank 200 through the air basket 563.
Specifically, in the embodiment of the present invention, as shown in fig. 9, the air receiving part 564 includes an outer housing 5641, a connection sleeve 5642 rotatably provided in the outer housing 5641, one end of the connection sleeve 5642 is connected to the air intake rod 562, and the other end is connected to the output end of the second power source 561; a closed air chamber 5643 is formed between the connecting sleeve 5642 and the outer shell 5641, a plurality of air holes 5644 are arranged on the connecting sleeve 5642, the air inlet interface 5645 is arranged on the outer shell 5641 and is communicated with the air chamber 5643, and air can enter the air inlet through hole 5621 through the air holes 5644.
In the embodiment shown in fig. 5, the air receiving member 564 is located on the upper side of the elevating platform 550, and at this time, the air receiving member 564 may be adhered to the upper side of the elevating platform 550, and then the second power source 561 may be disposed on the elevating platform 550 through the support frame 565. When the second power source 561 works, the second power source 561 drives the air inlet rod 562 to rotate through the connecting sleeve 5642, so that the air basket 563 is driven to rotate, meanwhile, air enters the air chamber 5643, then enters the air inlet through hole 5621 from the air hole 5644, then enters the air basket 563, finally, the air basket 563 blows into the drug delivery tank 200, and therefore the air basket 563 moves and meanwhile, the air enters. Therefore, when the lifting platform 550 moves up and down, the air blowing unit 560 is driven to move up and down, so that the position of the air basket 563 in the medicine feeding tank 200 can be adjusted, and meanwhile, the air basket 563 can also move up and down when rotating, so that the environment in the medicine feeding tank 200 is closer to the environment in the oral cavity when rinsing, and the transdermal diffusion experiment result has higher accuracy.
In order to reduce the volume of the blowing device 500, so that the structure of the blowing device 500 is more compact and has better structural stability, in the embodiment of the present invention, the driving gear 541 is centrally provided with a second through hole 5413, the second through hole 5413 penetrates through the first rotating shaft 5411, and the driven wheel 533 of the first transmission unit 530 is centrally provided with a third through hole 5331, and the air intake rod 562 can penetrate through the driving gear 541 from the second through hole 5413 and penetrate through the driven wheel 533 from the third through hole 5331, i.e., the air intake rod 562, the driving gear 541 and the driven wheel 533 are in a coaxial state.
The control module is connected to the first power source 520 and the second power source 561 so that the first power source 520 and the second power source 561 can be controlled. And for the control module, it may be disposed on the base 510, or be a separate external module, in which two power sources are connected when performing experiments. The control module can comprise a controller and a touch screen, so that the actions of the two power sources can be controlled according to experimental requirements.
Further, when the air blowing device 500 is provided to the medicine feeding pond 200, there is a problem in that it is inconvenient to sample the medicine feeding pond 200, and thus in order to solve the sampling problem, a first sampling tube 210 is provided to the medicine feeding pond 200, one end of the first sampling tube 210 is communicated with the medicine feeding pond 200, and the other end is provided with a first sampling port 211, specifically, as shown in fig. 2, the first sampling tube 210 is inclined upward, and the first sampling port 211 is higher than the upper end surface of the medicine feeding pond 200. In the existing diffusion cell structure, the size of the sampling tube is relatively long, which also results in a long needle of the required sampler to sample, so that in order to facilitate sampling, a first cavity 220 is provided on the first sampling tube 210, the first cavity 220 is communicated with the first sampling tube 210, when the needle of the sampler is short, the diffusion cell can be inclined during sampling, so that the solution in the drug administration cell 200 enters the first cavity 220, and then the sampling distance of the sampler is shortened, thereby facilitating sampling. However, when the air is blown into the dosing tank 200, if the air flow is excessively large, the solution is blown out from the sampling port, and thus, in the embodiment of the present invention, the first return pipe 240 is provided on the dosing tank 200, one end of the first return pipe 240 communicates with the dosing tank 200, and the other end communicates with the first containing chamber 220, so that the solution can flow back when the air is blown, avoiding overflowing.
Based on the same purpose, in the embodiment of the invention, the receiving tank 100 is provided with the second sampling tube 110, one end of the second sampling tube 110 is communicated with the receiving tank 100, the other end of the second sampling tube 110 is provided with the second sampling port 111, the second sampling tube 110 is inclined upwards, and the position of the second sampling port 111 is higher than the upper end of the receiving tank 100, so that the overflow of the solution from the sampling port in the experiment is avoided. In order to facilitate sampling, a second receiving chamber 120 is provided on the second sampling tube 110, and the second receiving chamber 120 is in communication with the second sampling tube 110.
Further, with the current diffusion cell, the drug delivery cell 200 is directly fastened to the receiving cell 100 and then clamped by a pipe clamp, but such a structure has many problems in that, on one hand, the drug delivery cell 200 must be accurately fastened to the receiving cell 100 to ensure that the positions of the drug delivery cell 200 and the receiving cell 100 are prevented from being greatly changed when clamped by the pipe clamp, and high requirements are put on the operation of experimenters; on the other hand, the solution easily leaks out from the gap between the drug delivery well 200 and the receiving well 100, and particularly when the solution in the drug delivery well 200 is disturbed, the solution more easily leaks out. Therefore, in order to solve the above-mentioned problems, in the embodiment of the present invention, as shown in fig. 10, the upper end surface of the receiving well 100 is provided with a plurality of annular grooves 130, and as shown in fig. 11, the lower end surface of the administration well 200 is correspondingly provided with a plurality of annular protrusions 230, and when the administration well 200 is fastened to the receiving well 100, the annular protrusions 230 are embedded in the corresponding annular grooves 130, as shown in fig. 2. By providing the annular protrusion 230 and the annular groove 130, on the one hand, the annular protrusion 230 and the annular groove 130 are mutually positioned, so that the administration tank 200 can be quickly buckled on the receiving tank 100 in the experiment, and the relative positions of the administration tank 200 and the receiving tank 100 cannot be changed; on the other hand, when clamped by the pipe clamp, the presence of the annular protrusion 230 and the annular groove 130 prevents the leakage of the solution, and particularly when the number of the annular protrusion 230 and the annular groove 130 exceeds 1, a labyrinth seal structure is formed, and the leakage is further reduced.
Further, in order to better simulate the environment in the oral cavity during rinsing, in the embodiment of the present invention, the air basket 563 can realize the following actions, the flow rate of the air basket 563 blowing outwards is constant, the air basket 563 rotates at a constant speed, the distance between the air basket 563 and the lower end surface of the drug delivery tank 200 is H cm, the upward or downward movement speed of the air basket 563 is V cm/s, v=kh in numerical value, wherein H > 0, k is 0.3-0.95, and the air basket 563 is uniformly accelerated during upward movement and uniformly decelerated during downward movement.
Further, since the impact pressure that the oral mucosa 300 can withstand during the ex vivo experiment is limited, in order to avoid disturbance of the solution in the administration reservoir 200 during the experimentMay cause excessive pressure on the oral mucosa 300, in order to avoid breakage of the oral mucosa 300, in the embodiment of the present invention, a pressure detection sensor 600 is disposed at the lower portion of the administration pond, and the pressure detection sensor 600 is connected to the control module. During the experiment, the maximum impact force P bearable by the oral mucosa can be tested in advance max The impact threshold of the oral mucosa is then determined based on the maximum impact force, e.g., in embodiments of the present invention, 0.5P is taken max ～0.8P max As the impact force threshold. In the experiment, if the pressure detected by the pressure detecting sensor 600 exceeds the impact force threshold value, the pressure is fed back to the control module, and the control module controls the first power source 520 and the second power source 561 to act, so that the air basket 563 is decelerated or far away from the oral mucosa 300, and the oral mucosa 300 is prevented from being damaged.
Based on the diffusion cell provided by the invention, a transdermal diffusion instrument is also provided, the experimental instrument comprises the diffusion cell and a water bath box, the transdermal diffusion process of oral mucosa during simulated rinsing can be performed by using the diffusion instrument, and compared with the existing experimental instrument, more accurate experimental results can be obtained.
The above examples are only preferred embodiments of the present invention, it being noted that: it will be apparent to those skilled in the art that several modifications and equivalents can be made without departing from the principles of the invention, and such modifications and equivalents fall within the scope of the invention.
Claims (13)
1. Transdermal diffusion cell for simulating a gargling process, for oral mucosa transdermal diffusion experiments, comprising a receiving cell (100) and a dosing cell (200), wherein the dosing cell (200) is buckled at the upper end of the receiving cell (100) and is communicated with the receiving cell (100), and an oral mucosa (300) is arranged between the receiving dosing cell (200) and the receiving cell (100), and the oral mucosa transdermal diffusion cell is characterized in that an air blowing device (500) is arranged at the upper end of the dosing cell (200), and the air blowing device (500) comprises:
a base (510) fastened on the drug delivery tank (200);
a first power source (520) provided on the base (510);
the first transmission unit (530) is connected with the output end of the first power source (520);
the second transmission unit (540) is arranged on the base (510) and connected with the first transmission unit (530), and the power of the first power source (520) is transmitted to the second transmission unit (540) through the first transmission unit (530);
the lifting table (550) is arranged on the second transmission unit (540), and the first power source (520) provides power for the up-and-down movement of the lifting table (550);
an air inlet unit (560) comprising a second power source (561) and an air inlet rod (562), wherein the second power source (561) is arranged on the lifting platform (550); an air inlet through hole (5621) penetrating along the axial direction is formed in the air inlet rod (562), an air basket (563) is arranged at one end of the air inlet rod (562), and the air inlet through hole (5621) is communicated with the air basket (563); the other end is connected with the output end of the second power source (561) through the gas receiving component (564); the air inlet rod (562) penetrates through the base (510) so that the air basket (563) is positioned in the medicine feeding pool (200), an air inlet interface (5645) used for being connected with an external air source is arranged on the air receiving component (564), and when the external air source supplies air, air can enter the air basket (563) through the air inlet through hole (5621) and blow into the medicine feeding pool (200) through the air basket (563);
and the control module is connected with the first power source (520) and the second power source (561).
2. The transdermal diffusion cell according to claim 1, wherein the gas receiving member (564) comprises an outer housing (5641), a connecting sleeve (5642) rotatably provided in the outer housing (5641), the connecting sleeve (5642) having one end connected to the gas intake rod (562) and the other end connected to the output of the second power source (561); a closed air chamber (5643) is formed between the connecting sleeve (5642) and the outer shell (5641), and a plurality of air holes (5644) are formed in the connecting sleeve (5642); the air inlet interface (5645) is arranged on the outer shell (5641) and is communicated with the air chamber (5643), and air can enter the air inlet through hole (5621) through the air hole (5644).
3. The transdermal diffusion cell according to claim 1, wherein the second transmission unit (540) comprises a driving gear (541) disposed on the base (510), at least two driven gears (542) disposed around the periphery of the driving gear (541) and meshed with the driving gear (541), a screw rod (543) is disposed on the driven gears (542), and the lifting table (550) is disposed on the screw rod (543) through a screw rod nut (551), and the driven gears (542) drive the screw rod (543) to rotate, so as to drive the lifting table (550) to move up and down.
4. A transdermal diffusion cell according to claim 3, wherein the base (510) is provided with a first through hole (511), a first rotating shaft (5411) is formed on the lower end surface of the driving gear (541), a first bearing (5412) is sleeved on the first rotating shaft (5411), and the first rotating shaft (5411) is disposed in the first through hole (511) through the first bearing (5412).
5. A transdermal diffusion cell according to claim 4, wherein the driving gear (541) has a second through hole (5413) formed in the center thereof, the second through hole (5413) penetrates the first rotary shaft (5411), and the air intake rod (562) penetrates the driving gear (541) from the second through hole (5413).
6. The transdermal diffusion cell according to claim 5, wherein the flow rate of the outward blowing of the air basket (563) is constant, the air basket (563) rotates at a constant speed, the distance from the air basket (563) to the oral mucosa (300) is H cm, the upward or downward moving speed of the air basket (563) is V cm/s, and V = kH, k is 0.3 to 0.96 in numerical value.
7. The transdermal diffusion cell according to claim 1, wherein the first transmission unit (530) includes a driving wheel (531) provided at an output end of the second power source (561), a driven wheel (533) provided on the driving gear (541), the driving wheel (531) and the driven wheel (533) are transmitted by a transmission belt (532), a third through hole (5331) is formed in a center of the driven wheel (533), and the air intake rod (562) passes through the third through hole (5331).
8. The transdermal diffusion cell of claim 1, wherein the insufflation device (500) further comprises a protective housing (570) disposed on the base (510), the first transmission unit (530) and the second transmission unit (540) being both located inside the protective housing (570).
9. The transdermal diffusion cell according to any one of claims 1-8, wherein a first sampling tube (210) and a first return tube (240) are provided on the drug delivery cell (200), one end of the first sampling tube (210) is communicated with the drug delivery cell (200), the other end of the first sampling tube is provided with a first sampling port (211), a first cavity (220) is provided on the first sampling tube (210), one end of the first return tube (240) is communicated with the drug delivery cell (200), and the other end of the first return tube is communicated with the first cavity (220).
10. The transdermal diffusion cell according to claim 9, wherein a second sampling tube (110) is provided on the receiving cell (100), one end of the second sampling tube (110) is communicated with the receiving cell (100), the other end of the second sampling tube is provided with a second sampling port (111), and a second containing cavity (120) is provided on the second sampling tube (110).
11. The transdermal diffusion cell according to claim 9, wherein the upper end face of the receiving cell (100) is provided with a plurality of annular grooves (130), the lower end face of the administration cell (200) is correspondingly provided with a plurality of annular protrusions (230), and when the administration cell (200) is fastened to the receiving cell (100), the annular protrusions (230) are embedded in the corresponding annular grooves (130).
12. The transdermal diffusion cell according to claim 9, wherein the drug delivery cell (200) is provided with a pressure detection sensor (600) at a lower end thereof, the pressure detection sensor (600) being connected to a control module.
13. A transdermal diffusion device for simulating a rinsing process, comprising a transdermal diffusion cell according to any one of claims 1 to 12.
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