CN102213655B - Full-automatic microbial staining film production equipment and staining method - Google Patents
Full-automatic microbial staining film production equipment and staining method Download PDFInfo
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- CN102213655B CN102213655B CN 201110060794 CN201110060794A CN102213655B CN 102213655 B CN102213655 B CN 102213655B CN 201110060794 CN201110060794 CN 201110060794 CN 201110060794 A CN201110060794 A CN 201110060794A CN 102213655 B CN102213655 B CN 102213655B
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Abstract
The invention discloses full-automatic microbial staining film production equipment and a staining method. The equipment is characterized in that the inner cavity of a cabinet is provided with a wash trough; a wash trough hoisting mechanism is connected between a motor and a wash trough; a water inlet pipe and a water outlet pipe stretch into the wash trough from the exterior of the cabinet, and a water inlet pipe solenoid valve and a water outlet pipe solenoid valve are respectively arranged on the water inlet pipe and the water outlet pipe; a micro liquid lever sensor is arranged on the inside wall of the wash trough; an annular ultraviolet tube is arranged at the top of the inner cavity of the cabinet and a pair of dust collection electrodes are arranged in the inside surface of the cabinet; a corona electrode in a hollow structure is positioned at the center of the pair of dust collection electrodes, a rotating disc type glass slide placing platform and a porous dropping dyeing plate which are coaxial and aligned are arranged right above the wash trough in sequence; an output pipeline containing a micro solenoid valve is evenly communicated at the bottom of each funnel-shaped reagent container; sterilization, ventilation and dust removal in the cabinet can be achieved; and the dyeing scheme and steps are programmed in advance and written into an automatic controller, the whole process of dyeing production is completely stimulated, different dyeing procedures are achieved through programming, each dyeing time and each washing time are precisely controlled, the unity and high quality dyeing effect are achieved.
Description
Technical field
The present invention relates to a kind of stained preparation technology, refer in particular to microorganism is dyeed and film-making, for detection of the high precision microorganism.
Background technology
Traditional microorganism detection method is to observe sampling after microorganism is carried out Gram’s staining or adopts capsule stain under optical microscope or fluorescent microscope at present.Because the microorganism of no dyeing and surrounding environment index of refraction difference are very little, therefore in the extremely difficult observation of microscopically.After dyeing, microorganism and environment form sharp contrast, can clearly observe form, arrangement and some architectural feature of microorganism, in order to classification and active the detection.
Most of dyeing and film-making work are all to adopt manually to complete at present, and whole dyeing course continues about ten minutes approximately, expend a large amount of manpower and materials, if it is many to observe in addition sample, dyeing time is long, and the work fatigue degree increases, and certainly will affect the working specification degree, thereby cause slice-making quality poor, affect accuracy of detection.China Patent No. is the automatic staining device that CN00106925.X discloses the dyeing use that is placed on the micro-censorship thing on slide glass, this device is furnished with a plurality of tandem reagent containers, the censorship phase continues and passes through reagent container, load a plurality of slide glasses with a transport basket, transport basket mentions and transports forward a stepping to enter in adjacent reagent container from corresponding reagent container by an electronic conveyer with lifting gear simultaneously, although this dyeing apparatus has been realized the batch dyeing of microslide, make dyeing efficient greatly improve, but this apparatus structure is complicated, and do not contain sterilization in device, the measures such as dirt of going out, make this device and be not suitable for the high precision microorganism detection such as bacterium, this device is not to be full-automatic in addition, lack the fixedly link to stained preparation, still have certain labor workload before use.
Summary of the invention
The objective of the invention is for overcoming above-mentioned the deficiencies in the prior art, provide a kind of simple in structure, aseptic, dustless, ultra-clean
AutomaticallyThe microbial staining pelletizer is simulated the overall process of stained preparation fully.The present invention also provides this simultaneously
MicroorganismColouring method
,Dye when can carry out the multi-disc slide glass simultaneously, and realize the accurate dyeing of coloured differently scheme by simple programming.
The technical scheme that full automatic microorganism stained preparation device of the present invention adopts is: have a cabinet, the cabinet inner chamber has kitchen sink, cabinet is fixed with motor outside the top, be connected with the kitchen sink hoisting gear between motor and kitchen sink, the kitchen sink hoisting gear has square support top, and affixed four links in four jiaos of bottoms of support top, four links pass respectively the kitchen sink of four perforation affixed bottoms at cabinet top, below, support top center sets firmly turbine, is connected motor with the worm screw that turbine matches; Water inlet pipe and water outlet pipe stretches into kitchen sink and sets up the inlet tube and outlet tube solenoid valve separately at water inlet pipe and water outlet pipe from the cabinet outside; The kitchen sink madial wall is provided with miniature liquid level sensor; The cabinet inner cavity top is provided with the ring-type ultraviolet lamp tube and medial surface is provided with a pair of dust collecting electrode, is the corona discharge electrode of hollow-core construction in the middle of a pair of dust collecting electrode, and corona discharge electrode top is connected with the arbor that stepper motor and gap cover have stepper motor; That the rotating disc type microslide mounting table of coaxial centering and porous are dripped and dyed dish successively directly over kitchen sink; Porous is dripped and is dyed dish and connects the cabinet top by link, and porous is dripped and dyed the dish gap and overlap corona discharge electrode is arranged; Porous is dripped and is dyed the rim it circumferentially is evenly equipped with 8 circular holes, and an infundibulate reagent container respectively is housed on each circular hole; Each infundibulate reagent container bottom all is communicated with the output pipe that miniature electromagnetic valve is housed, and reflective photoelectric sensor is equipped with on the miniature electromagnetic valve side; The affixed stepper motor of rotating disc type microslide mounting table and circumferentially be evenly equipped with 8 microslide standing grooves corresponding with infundibulate reagent container center; Motor, stepper motor, ultraviolet lamp tube, dust collecting electrode, corona discharge electrode, inlet tube and outlet tube solenoid valve are connected with miniature electromagnetic valve with control line connection self-actuated controller.
Full automatic microorganism colouring method of the present invention is: the scheme that will dye and step programming in advance write self-actuated controller, comprise the steps: A, open ultraviolet lamp tube to sterilizing in cabinet, opening fan keeps ventilating in cabinet, adopt a pair of dust collecting electrode and corona discharge electrode to dedusting in cabinet, required dyeing liquor is added in 8 infundibulate reagent containers successively, will put into respectively the microslide standing groove of microslide mounting table until the bacterium drop of microscopy after the microslide middle position.B, open No. 1 miniature electromagnetic valve until corresponding No. 1 dyeing liquor is closed after dropping on microslide, stepper motor drives the rotation of microslide mounting table, stepper motor stops the rotation after corresponding reflective photoelectric sensor detects next microslide signal, by this repetition until stop after the stepper motor rotating 360 degrees.C, motor rotation and kitchen sink being promoted by the kitchen sink hoisting gear is washed after clear water in it floods the microslide mounting table, motor reverse rotation afterwards makes kitchen sink be down to original position, opening the rising pipe solenoid valve discharges water, close when miniature liquid level sensor can't detect signal and open the water inlet pipe solenoid valve after the rising pipe solenoid valve and send water to kitchen sink, direct current generator drives fan and rotates by heating wire and send hot blast to make microslide dry in cabinet simultaneously.D, No. 2 miniature electromagnetic valves are opened automatically until close after dropping in corresponding No. 2 dyeing liquors on microslide, stepper motor drives the rotation of microslide mounting table, after detecting next microslide signal, corresponding reflective photoelectric sensor stops the rotation, continue to open miniature electromagnetic valve No. 2, close after dropping in corresponding No. 2 dyeing liquors on microslide, wash and change water by this repetition until rerun after the stepper motor rotating 360 degrees.
Compared with the prior art the present invention has following advantage:
1, simulate the overall process of stained preparation fully, can accurately control the time of each dyeing and washing, can realize unified and high-quality Color.
2, adopted rotating disc type to drip and dyed structure, dyeing when can carry out the multi-disc slide glass simultaneously.And realize the accurate dyeing of coloured differently scheme by simple programming.
3, have the removable kitchen sink that changes in real time water, thereby guarantee that washing quality reduces pollution.
4, have heating and ventilation unit, the indoor temperature of assurance device, thereby the time of the drying after each washing in the minimizing dyeing course.
5, have ultraviolet virus killing device and electrostatic precipitator in the device, guarantee the working environment of aseptic, the dustless and ultra-clean stained preparation that whole device is indoor, thereby significantly improve the microscopy precision.
6, apparatus of the present invention are applicable to any dyeing course, can realize different staining procedures by programming.
7, set up good working interface, can realize simply and easily the full automatic working of various dyeing courses.
Description of drawings
The main TV structure figure of Fig. 1 apparatus of the present invention;
Fig. 2 is the structure enlarged drawing of kitchen sink hoisting gear 4 in Fig. 1;
Fig. 3 is the structure enlarged drawing of electrostatic precipitator in Fig. 1;
Fig. 4 is that in Fig. 1, porous is dripped the vertical view that dyes dish 8 amplifications;
Fig. 5 is infundibulate reagent container 9 and associated components syndeton enlarged diagram thereof in Fig. 1;
Fig. 6 is the vertical view that Fig. 1 transfer disc type microslide mounting table 11 amplifies;
Fig. 7 is the Gram’s staining method flow diagram; Wherein, Fig. 7 (a) is that device shown in Figure 1 is operated in the working routine main flow chart under the Gram’s staining state; Fig. 7 (b) is the specific works flow process figure of wash module; Fig. 7 (c) is the specific works flow process figure under drying regime:
In Fig. 1-6: 1. cabinet; 2. bleeder vent; 3. ring-type ultraviolet lamp tube; 4. kitchen sink hoisting gear; 5. motor; 6. stepper motor; 7. dust collecting electrode; 8. porous is dripped and is dyed dish; 9. infundibulate reagent container; 10. corona discharge electrode; 11. rotating disc type microslide mounting table; 12. water inlet pipe and water outlet pipe; 13. kitchen sink; 14. heating wire; 15. fan; 16. direct current generator; 17. microslide standing groove; 18. rising pipe solenoid valve; 19. water inlet pipe solenoid valve; 20. miniature liquid level sensor; 21. bleeder vent; 22. spiral cover; 23. revolve mouth; 24. miniature electromagnetic valve; 25. output pipe; 26. self-actuated controller; 27. turbine; 28. reflective photoelectric sensor; 29. worm screw; 30. support top; 31. link; 32. circular hole; 33. arbor; 34. circular hole; 35. metal link; 36. perforation.
Embodiment
Referring to Fig. 1, the outer body of full-automatic rapid dyeing pelletizer of the present invention is a cabinet 1, cabinet 1 is comprised of tempered glass, can facilitate like this user to watch the duty of cabinet 1 inside, the top of cabinet 1 and bottom are provided with bleeder vent 2, bleeder vent 2 is communicated with cabinet 1 inner chamber, is convenient to the circulation of room air.Ring-type ultraviolet lamp tube 3 is equipped with at top at cabinet 1 inner chamber, to carrying out disinfection in cabinet 1 inner chamber, thereby makes inner chamber keep aseptic state.Be provided with heating wire 14, fan 15, kitchen sink 13, rotating disc type microslide mounting table 11 etc. in cabinet 1 inner chamber.Direct current generator 16 is equipped with in bottom at cabinet 1 inner chamber, direct current generator 16 output shafts connect the fan 15 on top, the top of fan 15 is heating wire 14 fixedly, driving fan 15 by direct current generator 16 rotates, fan 15 acts on heating wire 14, send hot blast by heating wire 14 to cabinet 1 inner chamber, cavity temperature in being used for improving, the oven dry link after accelerating to wash.The top of heating wire 14 is kitchen sinks 13, kitchen sink 13 tops are fixedly connected with kitchen sink hoisting gear 4, be fixed with motor 5 outside the top of cabinet 1, be connected with kitchen sink hoisting gear 4 between motor 5 and kitchen sink 13, motor 5 can promote or reduce the position of kitchen sink 13 by kitchen sink hoisting gear 4, thereby controls the time of washing.The water inlet pipe and water outlet pipe 12 of kitchen sink 13 stretches into kitchen sink 13 from cabinet 1 outside, be installed into respectively water solenoid valve 19 and rising pipe solenoid valve 18 on water inlet pipe and water outlet pipe 12, miniature liquid level sensor 20 is housed on the madial wall of kitchen sink 13, be used for controlling solenoid valve 19 and rising pipe solenoid valve 18, thereby realize the automatic water-replacing function.
Referring to Fig. 1 and 2, kitchen sink hoisting gear 4 mainly is comprised of square support top 30, turbine 27, worm screw 29 and link 31, wherein square support top 30 is positioned at topmost, be welded with four steel links 31 under its four angle, these four steel links 31 pass respectively four perforation 36 at cabinet 1 top, fix with four welded corner joints on the kitchen sink 13 of bottom.Turbine 27 is fixedly welded under support top 30 centres, the arbor fixed connection of worm screw 29 and motor 5.When motor 5 drives worm screw 29 rotation, because the effect of clamping down on of four perforation 36 at cabinet 1 top makes support top 30 and turbine 27 all keep static, make so whole support top 30 because the relative motion of turbine 27 and worm screw 29 is lifted or descends, thereby drive rising and the decline of kitchen sink 13.
Referring to Fig. 1 and 3, in cabinet 1, electrostatic precipitator is housed, electrostatic precipitator comprises a pair of dust collecting electrode 7, corona discharge electrode 10 and stepper motor 6, a pair of dust collecting electrode 7 is arranged on cabinet 1 medial surface, be corona discharge electrode 10 in the middle of a pair of dust collecting electrode 7, the horizontal level of a pair of dust collecting electrode 7 is roughly parallel with corona discharge electrode 10.The top of corona discharge electrode 10 connects stepper motor 6, and corona discharge electrode 10 is hollow-core construction, and the gap is enclosed within arbor 33 outsides of stepper motor 6, and the base of stepper motor 6 is fixed on its top.The diameter of corona discharge electrode 10 is much larger than the diameter of arbor 33, so corona discharge electrode 10 is not subjected to the impact of its rotation not with arbor 33 rotations of stepper motor 6 yet.Dust collecting electrode 7 and corona discharge electrode 10 are worked together, are used for electrostatic precipitation.Corona discharge electrode 10 is connected to the positive pole of high voltage direct current, a pair of dust collecting electrode 7 is connected to the negative pole of high voltage direct current, keep an electrostatic field that is enough to make gas ionization, when dusty gas forms inhomogeneous field by corona discharge electrode 10 and a pair of dust collecting electrode 7, ionize under the highfield effect around discharge electrode, form gaseous ion and electronics and make powder dust particle charged, particle after charged is moving and is depositing on a pair of dust collecting electrode 7 to dust collector pole under the electric field force effect, thereby reaches separating of dust and gas.A pair of dust collecting electrode 7 is designed to waveform, is conducive to grit deposition, and secondary fly-up is few, can effectively capture the powder dust particle of submicron order, therefore can guarantee dustfree environment in the dyeing chamber of cabinet 1.
Referring to Fig. 1,4 and 5, Fig. 4 is that porous is dripped the vertical view that dyes dish 8, porous drip dye the dish 8 be positioned at kitchen sink 13 directly over, it is circular holes 34 that porous is dripped the centre of dying dish 8, the diameter of circular hole 34 is greater than the diameter of corona discharge electrode 10, corona discharge electrode 10 passes circular hole 34, porous is dripped dye dish 8 gaps to be placed in outside corona discharge electrode 10.Cabinet 1 top is fixedly connected with metal link 35 downwards, and link 35 bottoms are fixedly connected with porous and drip and dye dish 8, and therefore, porous is dripped and dyed dish 8 and there is no annexation with corona discharge electrode 7, just is suspended in middle part in cabinet 1 by 35 welding of metal link.Porous is dripped and is dyed dish and 8 circumferentially be evenly equipped with 8 circular holes 32 along it, is separately installed with infundibulate reagent container 9 on 8 circular holes 32.Be the structural representation of infundibulate reagent container 9 as Fig. 5, the top of infundibulate reagent container 9 is spiral covers 22, and spiral cover 22 is provided with bleeder vent 21, and infundibulate reagent container 9 lateral surfaces are provided with and revolve mouthfuls 23, is used for that it is arranged on porous and drips and dye dish 8.The bottom of infundibulate reagent container 9 is communicated with output pipe 25, miniature electromagnetic valve 24 is housed on output pipe 25, be used for controlling the break-make of output pipe 25, in the bottom of every infundibulate reagent container 9, miniature electromagnetic valve 24 is installed, reflective photoelectric sensor 28 is equipped with on the side of miniature electromagnetic valve 24, is used for finding the exact position of the microslide that is placed on infundibulate reagent container 9 bottoms.
Referring to Fig. 1,4 and 6, Fig. 6 is the vertical view of rotating disc type microslide mounting table 11, directly over rotating disc type microslide mounting table 11 be porous drip dye the dish 8, be rotating disc type microslide mounting table 11 be arranged on porous drip dye the dish 8 and kitchen sink 13 between, rotating disc type microslide mounting table 11 is welded and fixed together with the end of the arbor 33 of stepper motor 6, and rotating disc type microslide mounting table 11 and porous are dripped to dye and coiled 8 coaxial centerings and be connected.Rotating disc type microslide mounting table 11 circumferentially is evenly equipped with 8 microslide standing grooves 17 along it, the center of this microslide standing groove 17 is corresponding with the center of infundibulate reagent container 9, thereby guarantees that dripping of each infundibulate reagent container 9 dye the middle position that the position is microslide.Rotating disc type microslide mounting table 11 drips with porous and dyes dish 8 with stepper motor 6 rotations, can accurately rotate a certain angle, and guarantees the accuracy of dying the position of dripping of each infundibulate reagent container 9.
Referring to Fig. 1, at the self-actuated controller 26 of the outer setting of cabinet 1, self-actuated controller 26 connects respectively a motor 5, stepper motor 6, another motor 16 with control line, ultraviolet lamp tube 3, dust collecting electrode 7, corona discharge electrode 10, heating wire 14, solenoid valve 18,19 and 24.Self-actuated controller 26 output steering orders are controlled the rotation of a motor 5, stepper motor 6 and another motor 16; Turn on and off the power supply of ultraviolet lamp tube 3, dust collecting electrode 7, corona discharge electrode 10 and heating wire 14; Drive to control solenoid valve 18,19 and 24 turn on and off.Self-actuated controller 26 just can be realized the dyeing of this dyeing scheme by order and time according to various dyeing scheme defineds to the corresponding control program of control object enforcement.Commonly used have Gram’s staining and a capsule stain.Their dyeing scheme and staining procedure programming in advance can be write self-actuated controller 26, the user only need just can directly start this dyeing course by the button of clicking on self-actuated controller 26, also can modify to dyeing course by the key-press programmable on self-actuated controller 26.
Referring to Fig. 1 and Fig. 7, when using, the present invention can at first according to the dyeing scheme, staining procedure be set, comprise: fix, just dye, mordant dyeing, decolour and redye five steps, wherein each step also comprises washing link and the front dry link of dyeing of changing in real time water, and, can realize the dyeing of other dyeing scheme by programming.The present invention introduces a specific embodiment of the present invention as an example of Gram’s staining commonly used example, and all the other staining procedures and the present embodiment are identical.Fig. 7 (a) is that device shown in Figure 1 is operated in the working routine main flow chart under the Gram’s staining state, at first open ultraviolet lamp tube 3, carry out indoor sterilizing, open the fan 15 of cabinet 1 bottom, keep cabinet 1 room ventilation, high voltage direct current is connected to a pair of dust collecting electrode 7 and corona discharge electrode 10, to the indoor dust removal process of carrying out of cabinet 1.After sterilization and dust removal process, required dyeing liquor is dripped according to porous the label that dyes on dish 8 add successively in infundibulate reagent container 9, No. 1 is ammonium oxalate crystal violet, and No. 2 is iodine liquid, and No. 3 is decolorant, and No. 4 is luxuriant red.To treat the middle position of the bacterium drop of microscopy at microslide, afterwards microslide is put into respectively the microslide standing groove 17 of microslide mounting table 11.Start the Gram’s staining pattern of self-actuated controller 26, at first device can start fixedly link, i.e. the fan 15 of cabinet 1 bottom and heating wire 14, and the set time is 1 minute.Then start and just dyes link: automatically opened No. 1 miniature electromagnetic valve 24 approximately 1 second, be about to the ammonium oxalate crystal violet dyeing liquor and drop in rear shut electromagnetic valve 24 on microslide, stepper motor 6 drives 11 rotations of microslide mounting table, stop the rotation the reflected signal of next microslide detected when the reflective photoelectric sensor 28 on No. 1 solenoid valve 24 sides after, then continued to open No. 1 miniature electromagnetic valve 24 approximately 1 second, be about to close miniature electromagnetic valve 24 after ammonium oxalate crystal violet drops on microslide, repeat by this step, after stepper motor 6 accumulative total rotating 360 degrees, quit work approximately 1 minute.afterwards, drive motor 5 rotations are also flooded microslide mounting table 11 by kitchen sink hoisting gear 4 with the clear water that kitchen sink 13 is promoted in kitchen sink 13, approximately 20s washing complete after, motor 5 reverse rotations also drop to original position by kitchen sink hoisting gear 4 with kitchen sink 13, open rising pipe solenoid valve 18 kitchen sink water is bled off this moment, closing rising pipe solenoid valve 18 after miniature liquid level sensor 20 can't detect signal opens water inlet pipe solenoid valve 19 and send water to kitchen sink 13, after miniature liquid level sensor 20 promptings reach normal water level, water inlet pipe solenoid valve 19 autoshutdowns.When kitchen sink 13 changed water, direct current generator 16 drove fan 15 and rotates by heating wire 14 to installing the indoor hot blast that send, acceleration microslide drying.Approximately after 1 minute, direct current generator 16 and heating wire 14 quit work.after this start the mordant dyeing link: No. 2 miniature electromagnetic valve 24 was opened approximately 1 second automatically, the iodine drop is closed miniature electromagnetic valve 24 No. 2 after on microslide, stepper motor 6 drives 11 rotations of microslide mounting table, stop the rotation the reflected signal of next microslide detected when the reflective photoelectric sensor 28 on No. 2 miniature electromagnetic valve 24 sides after, then continued to open No. 2 solenoid valves 24 approximately 1 second, the iodine drop is closed miniature electromagnetic valve 24 No. 2 after on microslide, repeat by this step, until after stepper motor 6 accumulative total rotating 360 degrees, quit work approximately 1 minute, rerun afterwards and wash and change the water program.Decolouring is with to redye for two steps basic identical with the front, and just after dripping off No. 3 decolorants, stepper motor 6 need not be shut down one minute, but directly starts washing procedure, and the pause time-delay of redying at last in addition after the sarranine dyeing of link is 2 minutes.
Claims (4)
1. full automatic microorganism stained preparation device, has a cabinet (1), it is characterized in that: cabinet (1) inner chamber has kitchen sink (13), cabinet (1) is fixed with motor (5) outside the top, be connected with kitchen sink hoisting gear (4) between motor (5) and kitchen sink (13), kitchen sink hoisting gear (4) has square support top (30), (30) four jiaos of affixed four links in bottom of support top (31), four links (31) pass respectively the kitchen sink (13) of four perforation (36) affixed bottom at cabinet (1) top, below, support top (30) center sets firmly turbine (27), the described motor (5) that is connected described support top (30) below with the worm screw (29) that turbine (27) matches, water inlet pipe and water outlet pipe (12) stretches into kitchen sink (13) and sets up inlet tube and outlet tube solenoid valve (19,18) separately at water inlet pipe and water outlet pipe (12) from cabinet (1) outside, kitchen sink (13) madial wall is provided with miniature liquid level sensor (20), cabinet (1) inner cavity top is provided with ring-type ultraviolet lamp tube (3) and medial surface is provided with a pair of dust collecting electrode (7), be the corona discharge electrode (10) of hollow-core construction in the middle of a pair of dust collecting electrode (7), corona discharge electrode (10) top is connected with the arbor (33) that stepper motor (6) and gap cover have stepper motor (6), that the rotating disc type microslide mounting table (11) of coaxial centering and porous are dripped and dyed dish (8) successively directly over kitchen sink (13), porous is dripped and is dyed dish (8) and described corona discharge electrode (10) without annexation, and porous is dripped and dyed dish (8) and be suspended in cabinet (1) interior middle part by metal link (35) welding, and described corona discharge electrode (10) passes described porous and drips the circular hole that dyes dish (8) centre, porous is dripped and is dyed dish (8) and circumferentially be evenly equipped with 8 circular holes (32) along it, and an infundibulate reagent container (9) respectively is housed on each circular hole (32), each infundibulate reagent container (9) bottom all is communicated with is equipped with the output pipe (25) of miniature electromagnetic valve (24), and reflective photoelectric sensor (28) is equipped with on miniature electromagnetic valve (24) side, the affixed stepper motor of rotating disc type microslide mounting table (11) (6) and circumferentially be evenly equipped with 8 microslide standing grooves (17) corresponding with infundibulate reagent container (9) center, described motor (5), stepper motor (6), ultraviolet lamp tube (3), dust collecting electrode (7), corona discharge electrode (10), inlet tube and outlet tube solenoid valve (19,18) are connected 24 with miniature electromagnetic valve) connect self-actuated controller (26) with control line respectively.
2. a kind of full automatic microorganism stained preparation device according to claim 1 is characterized in that: infundibulate reagent container (9) lateral surface be provided with revolve mouthful (23), the top is spiral cover (22), spiral cover (22) is provided with bleeder vent (21).
3. a kind of full automatic microorganism stained preparation device according to claim 1, it is characterized in that: cabinet (1) intracavity bottom is provided with direct current generator (16), direct current generator (16) top is connected fan (15), fan (15) top is heating wire (14), heating wire (14) top is kitchen sink (13), and described heating wire (14) is connected 16 with direct current generator) connect self-actuated controller (26) with control line respectively.
4. a kind of full automatic microorganism stained preparation device according to claim 1, is characterized in that: corona discharge electrode (10) connection high voltage direct current positive pole, a pair of dust collecting electrode (7) connection high voltage direct current negative pole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110060794 CN102213655B (en) | 2011-03-15 | 2011-03-15 | Full-automatic microbial staining film production equipment and staining method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110060794 CN102213655B (en) | 2011-03-15 | 2011-03-15 | Full-automatic microbial staining film production equipment and staining method |
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| CN102213655A CN102213655A (en) | 2011-10-12 |
| CN102213655B true CN102213655B (en) | 2013-06-12 |
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| CN 201110060794 Expired - Fee Related CN102213655B (en) | 2011-03-15 | 2011-03-15 | Full-automatic microbial staining film production equipment and staining method |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102519775A (en) * | 2011-11-15 | 2012-06-27 | 深圳市科软科技有限公司 | Automatic slide stainer |
| CN102768141B (en) * | 2012-07-19 | 2014-08-13 | 广州市达诚医疗技术有限公司 | Dyeing bin |
| CN107255585A (en) * | 2017-06-30 | 2017-10-17 | 柳州市全品贸易有限公司 | Gram's staining glass slide dyeing drying integral machine |
| CN109342156B (en) * | 2018-10-29 | 2021-01-15 | 重庆英特力科技有限公司 | Automatic slice dyeing device |
| CN109283033A (en) * | 2018-12-04 | 2019-01-29 | 天津市康婷生物工程集团有限公司 | A kind of HE dyeing apparatus suitable for Various Tissues batch dyeing |
| CN110031280B (en) * | 2019-05-24 | 2024-02-27 | 遵义医科大学 | Spore dyeing device |
| CN114207403B (en) * | 2020-03-11 | 2022-11-22 | 贵州美鑫达医疗科技有限公司 | Devices and assemblies for automated tissue processing and staining and uses thereof |
| WO2021180108A1 (en) * | 2020-03-11 | 2021-09-16 | Guizhou Meixinda Medical Technologies Co., Ltd. | Devices and components for automated tissue processing and staining and uses thereof |
| CN111487110B (en) * | 2020-04-28 | 2023-01-31 | 太原市罗塞塔石生物技术有限公司 | Gas circuit and liquid circuit integrated module for attaching glass slide |
| CN112763294B (en) * | 2020-12-29 | 2023-09-08 | 广东金泉医疗科技有限公司 | Heat treatment module and automatic drop dyeing sealing piece equipment with same |
| CN113515077A (en) * | 2021-04-23 | 2021-10-19 | 重庆德方信息技术有限公司 | System and method for monitoring human body cell staining process |
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| CN2543056Y (en) * | 2002-05-20 | 2003-04-02 | 余向东 | Immunohistochemistry dyeing automatic controller |
| WO2003031943A1 (en) * | 2001-10-06 | 2003-04-17 | Leica Mikrosysteme Gmbh | Device and method for rapid tissue preparations for histological investigations |
| CN2711719Y (en) * | 2004-05-28 | 2005-07-20 | 付志祥 | Automatic specimen coloring machine |
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- 2011-03-15 CN CN 201110060794 patent/CN102213655B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003031943A1 (en) * | 2001-10-06 | 2003-04-17 | Leica Mikrosysteme Gmbh | Device and method for rapid tissue preparations for histological investigations |
| CN2543056Y (en) * | 2002-05-20 | 2003-04-02 | 余向东 | Immunohistochemistry dyeing automatic controller |
| CN2711719Y (en) * | 2004-05-28 | 2005-07-20 | 付志祥 | Automatic specimen coloring machine |
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