CN109342156B - Automatic slice dyeing device - Google Patents
Automatic slice dyeing device Download PDFInfo
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- CN109342156B CN109342156B CN201811271684.3A CN201811271684A CN109342156B CN 109342156 B CN109342156 B CN 109342156B CN 201811271684 A CN201811271684 A CN 201811271684A CN 109342156 B CN109342156 B CN 109342156B
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- 238000004043 dyeing Methods 0.000 title claims abstract description 210
- 239000007788 liquid Substances 0.000 claims abstract description 89
- 239000011521 glass Substances 0.000 claims abstract description 64
- 238000002347 injection Methods 0.000 claims abstract description 22
- 239000007924 injection Substances 0.000 claims abstract description 22
- 238000004140 cleaning Methods 0.000 claims abstract description 15
- 238000007599 discharging Methods 0.000 claims abstract description 10
- 238000010186 staining Methods 0.000 claims description 57
- 230000007246 mechanism Effects 0.000 claims description 43
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 34
- 229910052742 iron Inorganic materials 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 16
- 238000001514 detection method Methods 0.000 claims description 10
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 230000001960 triggered effect Effects 0.000 claims description 3
- 230000036541 health Effects 0.000 abstract description 2
- 230000002572 peristaltic effect Effects 0.000 description 17
- 230000008569 process Effects 0.000 description 12
- 239000000243 solution Substances 0.000 description 12
- 230000033001 locomotion Effects 0.000 description 9
- 239000012192 staining solution Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 239000000975 dye Substances 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000005389 magnetism Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses an automatic dyeing device, which relates to the field of medical and health clinical examination equipment and comprises a vertically arranged dyeing tank with an opening at the upper part, wherein the dyeing tank is communicated with a liquid inlet pipe for injecting dyeing liquid and a liquid injection pipe for injecting cleaning liquid, the dyeing tank is also communicated with a liquid discharge pipe for discharging liquid, and an electromagnetic valve is arranged in the liquid discharge pipe; vertical plates are arranged on two sides of the dyeing tank, and sliding grooves are formed in the positions, opposite to the two vertical plates, of the two vertical plates; the sliding groove is connected with a sliding rod in a sliding mode, the lower portion of the sliding rod is provided with a reticular dyeing frame for glass slides to insert, the upper portion of the dyeing frame is provided with an opening, the dyeing frame can extend into the dyeing tank, and the bottom of the dyeing frame is provided with a rubber pad in sliding connection with the inner wall of the dyeing tank; the liquid inlet pipe, the liquid injection pipe and the liquid discharge pipe are all communicated with the side wall of the dyeing pond; the bottom of the dyeing tank is communicated with an air inlet pipe, and one end, far away from the dyeing tank, of the air inlet pipe is communicated with an air outlet of an air heater.
Description
Technical Field
The invention relates to the field of medical and health clinical examination equipment, in particular to an automatic staining device.
Background
In medical testing and biological research, there is often a need to make cell samples for better visualization by sectioning, staining, washing, and drying the cell samples. For dyeing, the existing biological and medical sample dyeing equipment comprises two main types of spray dyeing and dip dyeing. The spray dyeing is to spray a dyeing solution to the sample glass slide through a spray head to realize the dyeing of the sample; the dip dyeing is to place the glass slide carrying the sample in a dyeing bath filled with a dyeing solution to realize the dyeing of the sample.
The existing dip dyeing device generally comprises: the micro staining pool is used for storing staining solution and a single slide glass carrying a sample and is provided with a liquid injection port and a liquid discharge port; the liquid injection pipe is connected with the liquid injection port and the dyeing liquid container or the flushing liquid container; the liquid discharge pipe is connected with the liquid discharge port and the outside of the dyeing device; the peristaltic pump is arranged on the liquid injection pipe, and the control end of the peristaltic pump is connected with the main control module; the dyeing liquid container is used for storing the dyeing liquid; a rinse liquid container for storing a rinse liquid; the light sensing device is arranged on the side surface of the micro staining pool and used for detecting the thickness of a sample carried on the glass slide and providing a detection signal to the main control module; the main control module is used for analyzing a detection signal of the thickness of the sample, controlling the dyeing time and controlling the operation of the peristaltic pump; and the display part of the display and key module is used for displaying the running state information of the main control module, and the key part of the display and key module is used for inputting control signals to the main control module.
Although the existing dip dyeing device can automatically change the required dyeing time according to the thickness of a sample, the following problems still exist:
1. when the glass slide is taken out of the micro staining pool manually, due to the fact that poor strength and direction are mastered, the glass slide is not guaranteed to be taken out of the micro staining pool vertically and upwards, the glass slide is prone to inclining and is scraped to the inner wall of the micro staining pool, and therefore a sample is prone to being scraped.
2. Utilize the glass slide washing after the flush fluid will dye, then with the flush fluid discharge back, can remain moisture in the trace dyeing pond, when next time need dye the glass slide, remaining moisture can dilute the concentration of the dyeing liquid of discharging again to can influence the dyeing effect.
Disclosure of Invention
The invention aims to provide an automatic staining device which can dry a staining pool after a glass slide is stained, so that the concentration of staining solution discharged next time is prevented from being diluted by residual moisture in the staining pool, and the staining effect is ensured.
In order to solve the technical problems, the basic scheme provided by the invention is as follows:
the automatic dyeing device comprises a vertically arranged dyeing tank with an opening at the upper part, the dyeing tank is communicated with a liquid inlet pipe for injecting dyeing liquid and a liquid injection pipe for injecting cleaning liquid, the dyeing tank is also communicated with a liquid discharge pipe for discharging liquid, and an electromagnetic valve is arranged in the liquid discharge pipe; vertical plates are arranged on two sides of the dyeing tank, and sliding grooves are formed in the positions, opposite to the two vertical plates, of the two vertical plates; the sliding groove is connected with a sliding rod in a sliding mode, the lower portion of the sliding rod is provided with a reticular dyeing frame for glass slides to insert, the upper portion of the dyeing frame is provided with an opening, the dyeing frame can extend into the dyeing tank, and the bottom of the dyeing frame is provided with a rubber pad in sliding connection with the inner wall of the dyeing tank; the liquid inlet pipe, the liquid injection pipe and the liquid discharge pipe are all communicated with the side wall of the dyeing pond; the bottom of the dyeing tank is communicated with an air inlet pipe, and one end, far away from the dyeing tank, of the air inlet pipe is communicated with an air outlet of an air heater.
The basic scheme has the beneficial effects that: inserting the carrier plate with the fixed sample into the dyeing frame, extending the dyeing frame into the dyeing tank, injecting a dyeing solution into the dyeing tank through the liquid inlet pipe, stopping injecting the dyeing solution when the injected dyeing solution reaches a certain amount, and opening the liquid discharge pipe to discharge the dyeing solution after the sample is dyed; then injecting a cleaning solution into the staining pool through a liquid injection pipe, and discharging the cleaning solution after cleaning the glass slide; then the hot air blower is started, hot air enters the space between the bottom of the dyeing frame and the bottom of the dyeing tank through the air inlet pipe, because the bottom of the dyeing frame is provided with the rubber pad which is in sliding connection with the inner wall of the dyeing tank, the dyeing frame and the dyeing tank form a structure similar to a cylinder, the rubber pad is equivalent to a piston, thereby the air current pushes the whole dyeing frame to move upwards, namely the dyeing frame and the sliding rod integrally move upwards along the sliding groove on the vertical plate, the sliding groove plays a guiding role, in the process of upward movement of the dyeing frame, the wind not only plays a role of driving the dyeing frame to move upward, but also can dry the inner wall of the dyeing tank, so as to avoid the concentration of the dyeing liquid discharged next time from being diluted by the residual water in the dyeing tank, compared with the method that the dyeing frame is pushed to move upwards directly by using the driving mechanism, the method that wind is used as power to push the dyeing frame to move upwards saves an additional driving mechanism and saves additional electric energy consumption; and secondly, the dyeing frame is slowly pushed upwards by wind power, so that compared with the situation that the dyeing frame is quickly pushed upwards by a driving mechanism, the damage to the glass slide and the sample caused by the violent shaking of the dyeing frame can be avoided. And finally, taking the glass slide out of the staining frame to realize microscopic examination of the glass slide, wherein in the process of directly taking the glass slide out of the staining pool, the staining frame is in a net shape, so that part of water remained on the glass slide can be drained.
Furthermore, arc-shaped grooves are formed in the positions, opposite to the two vertical plates, of the two vertical plates, one ends of the arc-shaped grooves are connected with the top ends of the sliding grooves, a disc coaxial with the arc-shaped grooves is arranged on one side of each vertical plate, the device further comprises a driving mechanism for driving the disc to rotate by a preset angle, and the driving mechanism is in signal connection with a controller; a cavity is formed in the vertical plate above the arc-shaped groove and is communicated with an air outlet of the air heater through an air supply pipe, and a plurality of downward-inclined through holes are formed in the cavity and one side of the vertical plate, facing the dyeing tank; a strip-shaped hole is formed in one side, facing the circle center, of the arc-shaped groove, an electromagnet extending into the arc-shaped groove through the strip-shaped hole is fixedly connected to the peripheral surface of the disc, a counting touch switch is arranged at the top of the sliding groove, and an iron block capable of being in contact with the counting touch switch is fixedly connected to one end, extending into the sliding groove, of the sliding rod; the counting touch switch and the electromagnet are in signal connection with the controller, the controller is further in signal connection with a timer, a time threshold value is preset by the controller, when the counting touch switch is triggered for an odd number of times, the controller simultaneously controls the electromagnet to be powered on and the driving mechanism to be started, when the driving mechanism rotates for a preset angle, the controller simultaneously controls the timer to start timing and the driving mechanism to stop rotating, when the timer counts the time threshold value, the controller controls the driving mechanism to be reversed to the original position, and then the electromagnet is controlled to be powered off and the driving mechanism is controlled to be closed.
In the process that the airflow pushes the dyeing frame to move upwards, the sliding rod is connected with the sliding groove in a sliding mode through the iron block, when the iron block touches the counting touch switch, the controller simultaneously controls the electromagnet to be powered on and the driving mechanism to be started, the powered electromagnet has magnetism, the electromagnet and the iron block are attracted together, the driving mechanism drives the disc to rotate, the disc drives the electromagnet to do circular motion along the strip-shaped hole, the electromagnet drives the iron block to do circular motion along the arc-shaped groove, the iron block drives the sliding rod to do circular motion around the circle center of the disc, so that the whole dyeing frame and the glass slide do circular motion around the circle center of the disc, in the process that the dyeing frame rotates along with the disc, hot air is always blown out from the through hole which the glass slide is inclined downwards, the hot air can be directly blown onto the glass slide, the drying of the upper portion of the glass slide and a sample is realized, and before the dyeing, the hot air blown upwards from the staining pool can be blown to the bottom surface of the glass slide, so that the bottom surface of the glass slide is dried.
After actuating mechanism rotates the preset angle, the controller controls the time-recorder to begin timing and actuating mechanism stall simultaneously, the hypothesis preset angle is 90 °, the dyeing frame that vertical setting was in the horizontality this moment before, in order to make things convenient for the staff to take out the slide glass from the dyeing frame, this section of time of timing time threshold value, be for enough time to supply the staff to take the slide glass in the dyeing frame, after the time threshold value was counted to the time-recorder, controller control actuating mechanism reversal to the normal position, namely let the disc turn around to the normal position, let the dyeing frame be in vertical state again, then control electro-magnet outage and actuating mechanism close, so that the dyeing frame gets back to the next slide glass in the dyeing pond again and dyes.
Furthermore, the electromagnetic valve and the air heater are in signal connection with the controller, and when the electromagnetic valve is closed, the controller controls the air heater to be started; when the driving mechanism is closed, the controller controls the hot air blower to be closed.
When the electromagnetic valve is closed, the cleaning liquid in the dyeing tank is completely discharged, and at the moment, the hot air blower is started to push the dyeing frame to move upwards by utilizing the airflow; when the driving mechanism is closed, the dyeing frame just rotates to a vertical state from a horizontal state at the moment, the controller controls the hot air blower to be closed, and air allowance is remained in the dyeing pond, so that the dyeing frame can slowly descend along the sliding groove to enter the dyeing pond.
Further, the staining frame comprises three concave frames with the same shape, a gap for inserting the glass slide is formed in the concave part of each concave frame, one end of each concave frame is connected with the sliding rod, and the other concave frame is connected between the other ends of the two concave frames.
The dyeing frame adopts the structure, when the glass slide is inserted into or taken out of the dyeing frame, the glass slide only extends into the crack of the concave frame, and the sample on the glass slide is not contacted with the dyeing frame, so that the sample on the glass slide is not scraped off due to the contact with the dyeing frame, and the dyeing frame vertically moves upwards under the pushing of the air flow, and in the moving process of the dyeing frame, the glass slide is clamped by the concave frame and cannot shake or incline, so the glass slide cannot contact with the inner wall of the dyeing pool, and the sample cannot be scraped off due to the contact with the inner wall of the dyeing pool.
Further, the electromagnetic valve is in signal connection with the controller; the side surface of the staining pool is provided with a light sensing device, the light sensing device can be aligned with a sample on a glass slide inserted into the staining pool, the light sensing device is used for detecting the thickness of the sample and sending a detection signal to the controller, the controller analyzes the detection signal to obtain staining time, the controller controls the timer to start timing after the liquid inlet pipe stops liquid inlet, and the controller controls the electromagnetic valve to be opened after the timing staining time is finished.
By adopting the design, for samples with different thicknesses, the thickness of the sample can be automatically detected through the light sensation detection device, the required dyeing time of the sample is changed according to the thickness of the sample, and the dyeing effect on the sample is enhanced.
Further, the dyeing pond is equipped with the overflow protection groove that surrounds the dyeing pond outward, and overflow protection tank bottom is equipped with the overflow hole, and the overflow protection groove passes through overflow hole and fluid-discharge tube intercommunication.
The setting of overflow protection groove can catch the liquid that overflows the dyeing pond, avoids liquid to spill outward and leak.
Drawings
FIG. 1 is a vertical cross-sectional view of an embodiment of the automatic slide dyeing apparatus of the present invention;
FIG. 2 is a schematic view of the structure of the chute and the arc-shaped slot of the embodiment of the automatic slide dyeing device of the invention shown in FIG. 1;
FIG. 3 is a cross-section of an arc-shaped slot of the embodiment of the automatic slide dyeing apparatus of the present invention shown in FIG. 1.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the device comprises a dyeing tank 1, a liquid inlet pipe 2, a liquid injection pipe 3, a liquid discharge pipe 4, an electromagnetic valve 5, a vertical plate 6, a sliding groove 7, a sliding rod 8, a dyeing frame 9, a rubber pad 91, a concave frame 92, an air inlet pipe 10, a hot air blower 11, an arc-shaped groove 12, a disc 13, a cavity 14, a through hole 15, a strip-shaped hole 16, an electromagnet 17, an iron block 18, a light sensing device 19 and an overflow protection groove 20.
As shown in figure 1, the automatic dyeing device comprises a vertical plate 6, wherein the vertical plate 6 is connected with a dyeing tank 1 through a connecting rod, the upper part of the dyeing tank 1 is opened, the dyeing tank 1 is communicated with a liquid inlet pipe 2 for injecting dyeing liquid and a liquid injection pipe 3 for injecting cleaning liquid, the dyeing tank 1 is also communicated with a liquid discharge pipe 4 for discharging liquid, and the liquid inlet pipe 2, the liquid injection pipe 3 and the liquid discharge pipe 4 are all communicated with the side wall of the dyeing tank 1; peristaltic pumps are arranged in the liquid inlet pipe 2 and the liquid injection pipe 3, and an electromagnetic valve 5 is arranged in the liquid discharge pipe 4. An air heater 11 is arranged below the dyeing tank 1, the dyeing tank 1 is communicated with an air outlet of the air heater 11 through an air inlet pipe 10, the electromagnetic valve 5, the peristaltic pump and the air heater 11 are in signal connection with a controller, the controller is also in signal connection with a timer, the controller in the embodiment adopts an STM-32 series single chip microcomputer, a timer with the model of PC-530, a peristaltic pump with the model of A-60-G and an electromagnetic valve with the model of ZCB-1/600. The dyeing pond 1 is externally provided with an overflow protection groove 20 surrounding the dyeing pond 1, the bottom of the overflow protection groove 20 is provided with an overflow hole, and the overflow protection groove 20 is communicated with the liquid discharge pipe 4 through the overflow hole.
As shown in fig. 1, a light sensing device 19 is disposed on a side surface of the staining pool 1, the light sensing device 19 can be aligned with a sample on a slide glass inserted into the staining pool 1, specifically, the light sensing device 19 in this embodiment is a transmission light sensing device 19, a transmitting end and a receiving end of transmission light are respectively located on front and rear side walls of the staining pool 1, the light sensing device 19 is configured to detect a thickness of the sample and send a detection signal to a controller, the controller analyzes the detection signal to obtain a staining time, when the liquid inlet pipe 2 stops liquid inlet, the controller controls a timer to start timing, and when the timing staining time is over, the controller controls the electromagnetic valve 5 to open.
As shown in fig. 1, the two vertical plates 6 are provided with sliding grooves 7 at opposite positions, the sliding grooves 7 are connected with sliding rods 8 in a sliding manner, the sliding rods 8 are provided with iron blocks 18 extending into the sliding grooves 7, the cross sections of the sliding grooves 7 are in a T shape, and the connecting structure of the sliding rods 8 and the iron blocks 18 is also in a T shape matched with the sliding grooves 7. The lower part of the sliding rod 8 is provided with a reticular staining frame 9 for glass slides to be inserted, the upper part of the staining frame 9 is provided with an opening, the staining frame 9 comprises three concave frames 92 with the same shape, a gap for glass slides to be inserted is formed in the concave part of each concave frame 92, one end of each concave frame 92 is connected with the sliding rod 8, the other concave frame 92 is connected between the other end of each concave frame 92, the staining frame 9 can extend into the staining pool 1, and the bottom of the staining frame 9 is provided with a rubber pad 91 which is in sliding connection with the inner wall of the staining pool 1.
As shown in fig. 1 and 2, the two vertical plates 6 are further provided with arc-shaped grooves 12 at opposite positions, one ends of the arc-shaped grooves 12 are connected with the top ends of the sliding grooves 7, discs 13 coaxial with the arc-shaped grooves 12 are arranged on both sides of the two vertical plates 6 away from the dyeing tank 1, and the dyeing tank further comprises a driving mechanism for driving the discs 13 to rotate by a preset angle, the driving mechanism is in signal connection with a controller, and the driving mechanism of the embodiment is a motor; a strip-shaped hole 16 is formed in one side, facing the circle center, of the arc-shaped groove 12, an electromagnet 17 extending into the arc-shaped groove 12 through the strip-shaped hole 16 is fixedly connected to the peripheral surface of the disc 13, as shown in fig. 3, the upper part of the cross section of the arc-shaped groove 12 is in a T shape, the lower part of the cross section of the arc-shaped groove 12 is the strip-shaped hole 16 into which the electromagnet 17 extends, and the strip-shaped hole 16 is communicated with the upper part of the arc-shaped groove 12 so that the electromagnet 17 extending into the arc-shaped groove 12 and an iron block 18 positioned in the arc-shaped groove; the top of spout 7 is equipped with the count touch switch, and iron plate 18 can contact with the count touch switch, and count touch switch and electro-magnet 17 and controller signal connection.
As shown in fig. 1, a cavity 14 is opened on the vertical plate 6 above the arc-shaped groove 12, the cavity 14 is communicated with an air outlet of the air heater 11 through an air supply pipe, and a plurality of downward-inclined through holes 15 are opened on one side of the cavity 14 and the vertical plate 6 facing the dyeing tank 1.
The controller is preset with a time threshold, when the counting touch switch is triggered for an odd number of times, the controller simultaneously controls the electromagnet 17 to be electrified and the driving mechanism to be started, when the driving mechanism rotates for a preset angle, the controller simultaneously controls the timer to start timing and the driving mechanism to stop rotating, when the timer counts the time threshold, the controller controls the driving mechanism to reversely rotate to the original position, then controls the electromagnet 17 to be powered off and the driving mechanism to be closed, and then controls the air heater 11 to be closed.
In this embodiment, the time for injecting the staining solution into the staining bath 1, discharging the staining solution, injecting the cleaning solution into the staining bath 1, and discharging the cleaning solution is set in advance, and assuming that the time for injecting the staining solution into the staining bath 1 is T1, the time for discharging the staining solution is T2, the time for injecting the cleaning solution into the staining bath 1 is T3, and the time for discharging the cleaning solution is T4. That is, after the peristaltic pump in the liquid inlet pipe 2 is started, the controller controls the peristaltic pump in the liquid inlet pipe 2 to be closed after the time T1; after the peristaltic pump in the liquid inlet pipe 2 is closed, the controller controls the timer to start timing, after the timing dyeing time is over, the controller controls the electromagnetic valve 5 to be opened, and after the electromagnetic valve 5 is opened, the controller controls the electromagnetic valve 5 to be closed after the time T2; when the peristaltic pump in the liquid injection pipe 3 is started, the controller controls the peristaltic pump in the liquid injection pipe 3 to be closed after the time T3; when the peristaltic pump in the liquid injection pipe 3 is closed, the controller controls the electromagnetic valve 5 to be started, and after the time T4, the controller simultaneously controls the electromagnetic valve 5 to be closed and the hot air blower 11 to be started.
The specific implementation process of the automatic slice dyeing device is as follows:
the carrier piece that will fix the good sample injects in dyeing frame 9, stretch into dyeing pond 1 with dyeing frame 9 again, start the peristaltic pump in feed liquor pipe 2, inject the dyeing liquor into dyeing pond 1 through feed liquor pipe 2, the rubber pad 91 of dyeing frame 9 bottom is stopped up intake pipe and dyeing pond intercommunication department this moment, so in the dyeing liquor can not get into the intake pipe, because the lateral wall intercommunication of feed liquor pipe 2 and dyeing pond 1, so get into the space on the dyeing liquor is located rubber pad 91 in the dyeing pond, through time T1, also be exactly that the dyeing liquor that injects reaches a certain amount after, the peristaltic pump in feed liquor pipe 2 is closed to the controller, stop injecting the dyeing liquor.
Slide glass is located staining pond 1 this moment, the thickness of sample on the light sense detection device automated inspection slide glass on the 1 lateral wall of staining pond, when sample thickness when thicker, the light that sees through the transmitted light of sample is weaker more, the light intensity of the transmitted light that the receiving terminal received is less, the controller changes the dyeing time to the sample through the light intensity that the receiving terminal received, after liquid inlet pipe 2 stops the feed liquor, controller control time-recorder begins the timing, after timing dyeing time, controller control solenoid valve 5 opens, also open fluid-discharge tube 4 and carry out the flowing back promptly.
After the time T2, namely after the dyeing liquid is completely discharged, the controller closes the electromagnetic valve 5 and starts the peristaltic pump in the liquid injection pipe 3, the cleaning liquid is injected into the dyeing tank 1 through the liquid injection pipe 3, and after the time T3, the controller controls the peristaltic pump in the liquid injection pipe 3 to be closed, and at the moment, the cleaning liquid is stopped to be fed; when the peristaltic pump in the liquid injection pipe 3 is closed, the controller controls the electromagnetic valve 5 to be started, namely, the cleaning liquid starts to be discharged, and after the time T4, the controller simultaneously controls the electromagnetic valve 5 to be closed and the hot air blower 11 to be started.
After the air heater 11 is started, hot air enters the space between the bottom of the dyeing frame 9 and the bottom of the dyeing tank 1 through the air inlet pipe 10, because the bottom of the dyeing frame 9 is provided with the rubber pad 91 which is in sliding connection with the inner wall of the dyeing tank 1, the dyeing frame 9 and the dyeing tank 1 form a structure similar to an air cylinder, and the rubber pad 91 is equivalent to a piston, the air flow pushes the whole dyeing frame 9 to move upwards, namely the dyeing frame 9 and the sliding rod 8 integrally move upwards along the sliding chute 7 on the vertical plate 6, the sliding chute 7 plays a role of guiding, in the process of moving upwards of the dyeing frame 9, the air not only serves as power to push the dyeing frame 9 to move upwards, but also can dry the inner wall of the dyeing tank 1, so that the concentration of dyeing liquid discharged next time is prevented from being diluted by residual moisture in the dyeing tank 1, and the air serves as power to push the dyeing frame 9 to move upwards, and relatively to directly push the dyeing frame, firstly, an additional driving mechanism is omitted, and additional electric energy consumption is saved; secondly, the dyeing frame 9 is slowly pushed upwards by wind power, and compared with the situation that the dyeing frame 9 is rapidly pushed upwards by a driving mechanism, the damage to the glass slide and the sample caused by severe shaking of the dyeing frame 9 can be avoided.
In the process that the airflow pushes the dyeing frame 9 to move upwards, the sliding rod 8 is connected with the sliding groove 7 in a sliding mode through the iron block 18, when the iron block 18 touches the counting touch switch, the controller simultaneously controls the electromagnet 17 to be electrified and the driving mechanism to be started, the electrified electromagnet 17 has magnetism, the electromagnet 17 and the iron block 18 are attracted together, the driving mechanism drives the disc 13 to rotate simultaneously, the disc 13 drives the electromagnet 17 to do circular motion along the strip-shaped hole 16, the electromagnet 17 drives the iron block 18 to do circular motion along the arc-shaped groove 12, the iron block 18 drives the sliding rod 8 to do circular motion around the circle center of the disc 13, therefore, the whole dyeing frame 9 and the glass slide do circular motion around the circle center of the disc 13, in the process that the dyeing frame 9 rotates along with the disc 13, hot air is always blown out from the obliquely downward through hole 15, the hot air can be directly blown onto the glass slide, and the upper portion of the, and before the dyeing frame 9 starts to rotate, the rubber pad 91 at the bottom of the dyeing frame 9 leaves the dyeing tank 1, and hot air blown upwards from the dyeing tank 1 can be blown to the bottom surface of the slide glass, so that the bottom surface of the slide glass is dried.
After the driving mechanism rotates for a preset angle, the controller simultaneously controls the timer to start timing and the driving mechanism to stop rotating, the preset angle is assumed to be 90 degrees, the vertically arranged staining frame 9 is in a horizontal state at the moment so that a worker can conveniently take out the slide glass from the staining frame 9 transversely, the time of the time threshold is timed, enough time is reserved for the worker to take the slide glass in the staining frame 9, after the timer times for the time threshold, the controller controls the driving mechanism to rotate reversely to the original position, namely the disc 13 rotates to the original position, the staining frame 9 is in a vertical state again, then the electromagnet 17 is controlled to be powered off and the driving mechanism is controlled to be closed, so that the staining frame 9 returns to the staining pool 1 again for a next slide glass to be stained.
Finally, the microscopic examination of the slide glass can be realized by taking out the slide glass from the staining frame 9, and compared with the method of directly taking out the slide glass from the staining pool 1, in the process of moving the staining frame 9 upwards, the moisture remained on the slide glass can be drained partially because the staining frame 9 is in a net shape; the dyeing frame 9 is vertically upward under the guiding action of the sliding chute 7, and in the process that the dyeing frame 9 moves upwards, the glass slide does not contact the inner wall of the dyeing pool 1, so that the glass slide is prevented from being directly taken out of the dyeing pool 1 to scrape the sample; when the staining frame 9 is taken out, the slide glass only extends into the crack of the concave frame 92, and the sample on the slide glass is not contacted with the staining frame 9, so that the sample on the slide glass is not scraped off due to the contact with the staining frame 9; and because the dyeing frame 9 moves vertically and upwards under the pushing of the air current, in the moving process of the dyeing frame 9, the glass slide is clamped by the concave frame 92 and cannot shake or incline, so that the glass slide cannot contact the inner wall of the dyeing tank 1 due to inclination, and the integrity of the sample is ensured.
When the driving mechanism is closed, the dyeing frame 9 just rotates to the vertical state from the horizontal state at the moment, the controller controls the hot air blower 11 to be closed, and air allowance is remained in the dyeing pool 1, so that the dyeing frame 9 can slowly descend along the sliding groove 7 to enter the dyeing pool 1, the dyeing frame 9 is prevented from directly descending to impact the dyeing pool 1, and another glass slide is inserted into the dyeing frame 9, and then the dyeing of the next glass slide is realized.
The foregoing is merely an example of the present invention and common general knowledge of known specific structures and features of the embodiments is not described herein in any greater detail. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (5)
1. The automatic dyeing device comprises a vertically arranged dyeing tank with an opening at the upper part, the dyeing tank is communicated with a liquid inlet pipe for injecting dyeing liquid and a liquid injection pipe for injecting cleaning liquid, the dyeing tank is also communicated with a liquid discharge pipe for discharging liquid, and an electromagnetic valve is arranged in the liquid discharge pipe; the method is characterized in that: vertical plates are arranged on two sides of the dyeing tank, and sliding grooves are formed in the positions, opposite to the two vertical plates, of the two vertical plates; the sliding groove is connected with a sliding rod in a sliding mode, the lower portion of the sliding rod is provided with a reticular dyeing frame for glass slides to insert, the upper portion of the dyeing frame is provided with an opening, the dyeing frame can extend into the dyeing tank, and the bottom of the dyeing frame is provided with a rubber pad in sliding connection with the inner wall of the dyeing tank; the liquid inlet pipe, the liquid injection pipe and the liquid discharge pipe are all communicated with the side wall of the dyeing pond; the bottom of the dyeing tank is communicated with an air inlet pipe, and one end of the air inlet pipe, which is far away from the dyeing tank, is communicated with an air outlet of an air heater;
the device comprises two vertical plates, a sliding groove and a driving mechanism, wherein the two vertical plates are arranged in a vertical mode, the two vertical plates are arranged in the sliding groove, the two vertical plates are opposite to each other, one end of the sliding groove is connected with the top end of the sliding groove, one side of each vertical plate is provided with a disc which is coaxial with the corresponding sliding groove, the device further comprises the driving mechanism which; a cavity is formed in the vertical plate above the arc-shaped groove and is communicated with an air outlet of the air heater through an air supply pipe, and a plurality of downward-inclined through holes are formed in the cavity and one side of the vertical plate, facing the dyeing tank; a strip-shaped hole is formed in one side, facing the circle center, of the arc-shaped groove, an electromagnet extending into the arc-shaped groove through the strip-shaped hole is fixedly connected to the peripheral surface of the disc, a counting touch switch is arranged at the top of the sliding groove, and an iron block capable of being in contact with the counting touch switch is fixedly connected to one end, extending into the sliding groove, of the sliding rod; the counting touch switch and the electromagnet are in signal connection with the controller, the controller is further in signal connection with a timer, a time threshold value is preset by the controller, when the counting touch switch is triggered for an odd number of times, the controller simultaneously controls the electromagnet to be powered on and the driving mechanism to be started, when the driving mechanism rotates for a preset angle, the controller simultaneously controls the timer to start timing and the driving mechanism to stop rotating, when the timer counts the time threshold value, the controller controls the driving mechanism to be reversed to the original position, and then the electromagnet is controlled to be powered off and the driving mechanism is controlled to be closed.
2. The automatic slide dyeing device according to claim 1, characterized in that: the electromagnetic valve and the air heater are in signal connection with the controller, and when the electromagnetic valve is closed, the controller controls the air heater to be started; when the driving mechanism is closed, the controller controls the hot air blower to be closed.
3. The automatic slide dyeing device according to claim 1, characterized in that: the staining frame comprises three concave frames with the same shape, a gap for inserting the glass slide is formed in the concave part of each concave frame, one end of each concave frame is connected with the corresponding sliding rod, and the other concave frame is connected between the other ends of the two concave frames.
4. The automatic slide dyeing device according to claim 1, characterized in that: the electromagnetic valve is in signal connection with the controller; the side surface of the staining pool is provided with a light sensing device, the light sensing device can be aligned with a sample on a glass slide inserted into the staining pool, the light sensing device is used for detecting the thickness of the sample and sending a detection signal to the controller, the controller analyzes the detection signal to obtain staining time, the controller controls the timer to start timing after the liquid inlet pipe stops liquid inlet, and the controller controls the electromagnetic valve to be opened after the timing staining time is finished.
5. The automatic slide dyeing device according to claim 1, characterized in that: the dyeing pond is equipped with the overflow protection groove that surrounds the dyeing pond outward, and overflow protection tank bottom is equipped with the overflow hole, and overflow protection groove passes through overflow hole and fluid-discharge tube intercommunication.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811271684.3A CN109342156B (en) | 2018-10-29 | 2018-10-29 | Automatic slice dyeing device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811271684.3A CN109342156B (en) | 2018-10-29 | 2018-10-29 | Automatic slice dyeing device |
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| CN109342156B true CN109342156B (en) | 2021-01-15 |
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| CN110132695B (en) * | 2019-05-27 | 2022-01-11 | 福州迈新生物技术开发有限公司 | Method for reducing abnormal dyeing caused by human error by pathological dyeing system |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2781351Y (en) * | 2005-04-14 | 2006-05-17 | 刘仁明 | Medical multi-smear coloring and drying two-use rack |
| CN2881595Y (en) * | 2005-10-19 | 2007-03-21 | 刘和录 | Dyeing dry plate device used for inspecting biological material |
| CN102213655A (en) * | 2011-03-15 | 2011-10-12 | 江苏大学 | Full-automatic microbial staining film production equipment and staining method |
| CN103674663A (en) * | 2013-12-03 | 2014-03-26 | 珠海贝索生物技术有限公司 | Dyeing instrument for flowing dip dyeing |
| CN203719997U (en) * | 2013-12-26 | 2014-07-16 | 韩亚超 | Bacteria staining device |
| CN205642973U (en) * | 2016-02-26 | 2016-10-12 | 武汉达尔英生物科技有限公司 | Gram dyeing instrument of antiaciding |
| CN206920213U (en) * | 2017-07-26 | 2018-01-23 | 付亚平 | A kind of full-automatic slide staining device of Portable sanitary |
| CN107794676A (en) * | 2017-10-25 | 2018-03-13 | 繁昌县清新水洗有限责任公司 | One kind dyeing drying integrated device |
| CN207540861U (en) * | 2017-12-21 | 2018-06-26 | 川北医学院 | A kind of full-automatic multi-functional glass slide dyeing instrument |
| CN108360184A (en) * | 2018-03-26 | 2018-08-03 | 陈雪莲 | Cloth dyeing device is boiled in a kind of weaving |
| CN207908239U (en) * | 2018-03-19 | 2018-09-25 | 遵义医学院附属医院 | A kind of cell or Bacterial stain device frame |
-
2018
- 2018-10-29 CN CN201811271684.3A patent/CN109342156B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2781351Y (en) * | 2005-04-14 | 2006-05-17 | 刘仁明 | Medical multi-smear coloring and drying two-use rack |
| CN2881595Y (en) * | 2005-10-19 | 2007-03-21 | 刘和录 | Dyeing dry plate device used for inspecting biological material |
| CN102213655A (en) * | 2011-03-15 | 2011-10-12 | 江苏大学 | Full-automatic microbial staining film production equipment and staining method |
| CN103674663A (en) * | 2013-12-03 | 2014-03-26 | 珠海贝索生物技术有限公司 | Dyeing instrument for flowing dip dyeing |
| CN203719997U (en) * | 2013-12-26 | 2014-07-16 | 韩亚超 | Bacteria staining device |
| CN205642973U (en) * | 2016-02-26 | 2016-10-12 | 武汉达尔英生物科技有限公司 | Gram dyeing instrument of antiaciding |
| CN206920213U (en) * | 2017-07-26 | 2018-01-23 | 付亚平 | A kind of full-automatic slide staining device of Portable sanitary |
| CN107794676A (en) * | 2017-10-25 | 2018-03-13 | 繁昌县清新水洗有限责任公司 | One kind dyeing drying integrated device |
| CN207540861U (en) * | 2017-12-21 | 2018-06-26 | 川北医学院 | A kind of full-automatic multi-functional glass slide dyeing instrument |
| CN207908239U (en) * | 2018-03-19 | 2018-09-25 | 遵义医学院附属医院 | A kind of cell or Bacterial stain device frame |
| CN108360184A (en) * | 2018-03-26 | 2018-08-03 | 陈雪莲 | Cloth dyeing device is boiled in a kind of weaving |
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