CN110132695B - Method for reducing abnormal dyeing caused by human error by pathological dyeing system - Google Patents
Method for reducing abnormal dyeing caused by human error by pathological dyeing system Download PDFInfo
- Publication number
- CN110132695B CN110132695B CN201910447746.XA CN201910447746A CN110132695B CN 110132695 B CN110132695 B CN 110132695B CN 201910447746 A CN201910447746 A CN 201910447746A CN 110132695 B CN110132695 B CN 110132695B
- Authority
- CN
- China
- Prior art keywords
- reagent
- result information
- detection result
- monitoring unit
- control unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000001575 pathological effect Effects 0.000 title claims abstract description 33
- 230000002159 abnormal effect Effects 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000004043 dyeing Methods 0.000 title claims abstract description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 132
- 238000001514 detection method Methods 0.000 claims abstract description 73
- 238000010186 staining Methods 0.000 claims abstract description 35
- 238000012544 monitoring process Methods 0.000 claims description 69
- 239000007788 liquid Substances 0.000 claims description 21
- 230000008439 repair process Effects 0.000 claims description 21
- 230000008569 process Effects 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000002378 acidificating effect Effects 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 239000000427 antigen Substances 0.000 description 6
- 102000036639 antigens Human genes 0.000 description 6
- 108091007433 antigens Proteins 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 238000003364 immunohistochemistry Methods 0.000 description 6
- 230000002055 immunohistochemical effect Effects 0.000 description 5
- 238000011161 development Methods 0.000 description 3
- 238000010827 pathological analysis Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002618 waking effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The invention relates to a method for reducing abnormal dyeing caused by human error for a pathological dyeing system, which comprises the steps of detecting a required reagent before the pathological dyeing system is started, and controlling the pathological dyeing system to be started by a control unit if a detection result is within a normal threshold range; if one detection result is not in the normal threshold range, the control unit cannot send a starting signal of the pathological staining system and sends a corresponding alarm signal to remind an operator to detect or replace the corresponding reagent again, so that the abnormal staining result caused by the performance problem of the reagent is avoided.
Description
Technical Field
The invention belongs to the field of medical detection, particularly relates to the technical field of immunohistochemistry and immunocytochemistry dyeing, and more particularly relates to a method for reducing abnormal dyeing caused by human errors by a full-automatic pathological dyeing system.
Background
Immunohistochemistry (IHC) is a new technology developed by combining the theory and technology of immunology on histochemical methods, and combines the theory and method of the subjects of immunology, histology and biochemistry, etc. The core of immunology, namely the principle of specific combination of antigen and antibody, is utilized, the antigen is tracked by using the antibody marked with enzyme, metal ion, isotope and the like, a display agent marked on the specific antibody combined with the antigen shows a certain color through chemical reaction, and the dyeing result is observed by means of a microscope, a fluorescence microscope and an electron microscope, so that the purpose of detecting the antigen is achieved.
Immunohistochemistry has a long history, and since 1940 Coons established immunofluorescence techniques to detect the corresponding antigens in frozen sections, there has been 73 history to date. In 1965, the immunohistochemical technique was first established in China, but the method was not widely applied to pathological diagnosis until the early 90 s in the 20 th century. The technique goes through 4 stages of development: establishing and developing an immunohistochemical technology; a popularization stage; clinical pathological diagnosis and application stage; quality control, normalization and standardization phases. Today, without the aid of immunohistochemistry, many tumors are poorly diagnosed and classified. Meanwhile, the wide application of immunohistochemistry in clinical pathological diagnosis also promotes the rapid development of immunohistochemical technology.
The main steps in the immunohistochemical staining process include: baking, deparaffinization, hydration, antigen retrieval, or peroxidase blocking (optional addition), primary, secondary or tertiary antibodies (detection system), DAB or hematoxylin (optional addition), and all rinses.
With the development of science and technology, various advanced technologies are also gradually applied to the immunohistochemical automatic staining process to replace manual staining. In order to overcome the difference of artificial dyeing results, an immunohistochemical automatic dyeing system is developed, and in recent years, full-automatic biochemical analyzers with different models and functions are in succession, so that the working efficiency and the detection accuracy are greatly improved.
However, in the running process of the full-automatic pathological staining system, different reagents are needed to be used in different procedures, and the factors influencing the normal staining of the full-automatic pathological staining system are mainly three, the first factor is a tissue section factor, the second factor is a system factor, and the third factor is a reagent factor. The first factor and the second factor can ensure the dyeing result to be normal through human intervention, but the used reagents have more types and larger property difference, so that the workload required by real-time detection is large, the time consumption is long, and the cost is high.
Disclosure of Invention
The invention aims to provide a method for reducing abnormal dyeing caused by human errors by a pathological dyeing system, which aims to solve the problem that the dyeing result is abnormal because the performance of a used reagent cannot be detected immediately in the prior art.
The invention is realized by the following technical scheme:
a pathological staining system method for reducing abnormal staining caused by human errors comprises the following steps:
respectively determining corresponding normal range threshold values according to the types of reagents required in the dyeing process, and storing the normal range threshold values;
a control unit of the pathological staining system receives a wake-up signal for starting the system, and the control unit sends a starting signal to an online monitoring control unit;
after the on-line monitoring unit receives the starting signal, the on-line monitoring unit controls the corresponding sensor to start, and the corresponding sensor transmits the detection result information of the corresponding reagent to the on-line monitoring unit;
the online monitoring unit compares the received detection result information with the threshold value of the corresponding type of reagent, and judges that:
if the detection result information is within the threshold range of the corresponding type of reagent, the online monitoring unit feeds back a normal signal to the control unit, and the control unit controls the pathological staining system to start;
and if the detection result information is not in the threshold range of the corresponding type of reagent, the online monitoring unit feeds back an abnormal signal to the control unit, and the control unit does not start the pathological staining system and sends a warning signal after receiving the abnormal signal.
The on-line monitoring unit compares the received detection result information with the threshold value of the corresponding type of reagent, and comprises the following steps:
determining the type N of the reagent, wherein N is a natural number;
1) the online monitoring unit judges the detection result information of the first reagent and the threshold value of the first reagent; if the detection result information of the first reagent is within the threshold range of the first reagent, the online monitoring unit carries out the next step;
if the detection result information of the first reagent is not in the threshold range of the first reagent, the online monitoring unit feeds back an abnormal signal to the control unit;
2) the online monitoring unit judges the detection result information of the second reagent and the threshold value of the second reagent;
if the detection result information of the second reagent is within the threshold range of the second reagent, the online monitoring unit carries out the next step;
if the detection result information of the second reagent is not in the threshold range of the second reagent, the online monitoring unit feeds back an abnormal signal to the control unit;
3) until the online monitoring unit judges the detection result information of the Nth reagent and the threshold value of the Nth reagent;
if the detection result information of the Nth reagent is within the threshold range of the Nth reagent, the online monitoring unit feeds back a normal signal to the control unit, and the control unit controls the pathological staining system to start;
and if the detection result information of the Nth reagent is not in the threshold range of the Nth reagent, the online monitoring unit feeds back an abnormal signal to the control unit.
The on-line monitoring unit compares the received detection result information with the threshold value of the corresponding type of reagent, and comprises the following steps:
determining the type N of the reagent, wherein N is a natural number;
the online monitoring unit receives the detection result information of the N reagents, compares the detection result information with the threshold values of the corresponding reagents respectively, and judges that:
if the detection result information of the N reagents is respectively in the corresponding reagent threshold value range, the online monitoring unit feeds back a normal signal to the control unit;
and if the detection result information of one reagent is not in the corresponding reagent threshold range, the online monitoring unit feeds back an abnormal signal to the control unit.
The detection result information of each reagent comprises pH value result information, and the threshold value range of the pH value of each reagent is the same or different.
The reagent comprises a plurality of detection auxiliary reagents, and the threshold range of the pH value of the detection auxiliary reagents is as follows:
(1) the pH value range of the acidic repair liquid is as follows: 6.0 plus or minus 0.2;
(2) the pH value range of the neutral repair liquid is as follows: 7.4 plus or minus 0.2;
(3) the pH value range of the alkaline repair liquid is as follows: 8.0 plus or minus 0.2 to 9.0 plus or minus 0.2;
(4) PBS buffer pH range: 7.4 plus or minus 0.2;
(5) distilled water pH range: 7.0 +/-0.5.
The reagent test result information includes Total Dissolved Solids (TDS) result information, and the threshold range of TDS values for each of the reagents is the same or different.
The threshold range of the TDS value is:
(1) the TDS of the acidic repair liquid is less than 1000;
(2) the TDS of the neutral repair liquid is less than 500;
(3) the TDS of the alkaline repair liquid is less than 200;
(4) TDS of PBS buffer solution is less than 6500;
(5) the TDS of the distilled water is less than 50.
The invention has the beneficial effects that:
according to the technical scheme, the required reagent is detected before the pathological staining system is started, and if the detection result is within a normal threshold range, the control unit controls the pathological staining system to be started; if one detection result is not in the normal threshold range, the control unit cannot send a starting signal of the pathological staining system and sends a corresponding alarm signal to remind an operator to detect or replace the corresponding reagent again, so that the abnormal staining result caused by the performance problem of the reagent is avoided.
Drawings
FIG. 1 is a logic diagram of the start-up control of the pathological staining system of the present invention.
Detailed Description
The technical solutions of the present invention are described in detail below by examples, and the following examples are only exemplary and can be used only for explaining and explaining the technical solutions of the present invention, but not construed as limiting the technical solutions of the present invention.
The application provides a method for reducing abnormal staining caused by human error by a pathological staining system, as shown in figure 1, comprising the following steps:
and determining corresponding normal range threshold values according to the types of reagents required in the dyeing process, and storing the normal range threshold values, wherein the specific storage position can be stored on a storage module of the control unit, a storage module of the on-line monitoring unit or an independent storage module.
Here, the reagents required for the entire staining process are set to N types, where N is a natural number, N threshold ranges correspond to N threshold ranges, and the threshold ranges correspond to the reagents one by one.
The control unit of the pathological staining system receives the wake-up signal for starting the system, and the control unit sends a starting signal to the on-line monitoring control unit. In this embodiment, the wake-up signal for starting the system may be implemented by a switch disposed on the body of the pathological staining system, or may wake up the control unit by a wireless signal, and the interaction mode between the wireless signal and the control unit may be implemented by a wireless interaction mode in the prior art, which is not described in detail herein.
After the control unit receives the wake-up signal, the control unit may further include power-on control before waking up the on-line monitoring unit, that is, the control unit controls the power-on of the pathological staining system first to enter a preparation stage of starting, and meanwhile, the on-line monitoring unit may also control the sensor to start after the power-on. In this embodiment, after the online monitoring unit is awakened by the control unit, the online monitoring unit controls the inductor to be powered on and enters a working state.
In the present application, the number of the sensors is at least N, where N is a natural number, which can achieve at least one-to-one correspondence with the types of the reagents, and of course, since the detection item of each reagent may be one or more, the number of the sensors may also be increased correspondingly. In other embodiments of the present application, an integrated sensor may be used, for example, to collect pH values of all kinds of reagents, and transmit different channels of the same information through one integrated sensor, so as to reduce the number of sensors and the volume or the number of lines of the pathological staining system.
After the sensors are started, the detection result information of the reagent corresponding to each sensor is transmitted to the online monitoring unit.
The on-line monitoring unit compares the received detection result information with the threshold value of the corresponding type of reagent, and judges that:
in the present application, two determination methods are included:
the first one is:
determining the type N of the reagent, wherein N is a natural number;
1) the online monitoring unit judges the detection result information of the first reagent and the threshold value of the first reagent; if the detection result information of the first reagent is within the threshold range of the first reagent, the online monitoring unit carries out the next step;
if the detection result information of the first reagent is not in the threshold range of the first reagent, the online monitoring unit feeds back an abnormal signal to the control unit;
2) the online monitoring unit judges the detection result information of the second reagent and the threshold value of the second reagent;
if the detection result information of the second reagent is within the threshold range of the second reagent, the online monitoring unit carries out the next step;
if the detection result information of the second reagent is not in the threshold range of the second reagent, the online monitoring unit feeds back an abnormal signal to the control unit;
3) until the online monitoring unit judges the detection result information of the Nth reagent and the threshold value of the Nth reagent;
if the detection result information of the first reagent is within the threshold range of the Nth reagent, the on-line monitoring unit feeds back a normal signal to the control unit, and the control unit controls and controls the pathological staining system to start;
and if the detection result information of the Nth reagent is not in the threshold range of the Nth reagent, the online monitoring unit feeds back an abnormal signal to the control unit.
And the second method comprises the following steps:
determining the type N of the reagent, wherein N is a natural number;
the online monitoring unit receives the detection result information of the N reagents, compares the detection result information with the threshold values of the corresponding reagents respectively, and judges that:
if the detection result information of the N reagents is respectively in the corresponding reagent threshold value range, the online monitoring unit feeds back a normal signal to the control unit;
and if the detection result information of one reagent is not in the corresponding reagent threshold range, the online monitoring unit feeds back an abnormal signal to the control unit.
As to which of the above-described determination methods is selected, the control method of the present application can be realized.
Here, the pH of each reagent is taken as an example for explanation, and usually, the detection result information of each reagent includes pH result information, and the threshold ranges of the pH of each reagent are the same or different.
Of course, the pH result information may be excluded from the detection result information of a certain reagent or reagents.
For example, in the present embodiment, the threshold range of the pH value of a certain alkaline repair liquid is 9.0 ± 0.2.
In the detection result information of the repair liquid, if the detected pH value is: the pH value is more than 8.8 and less than 9.2, which indicates that the result information of the repair liquid is within the threshold value, and at the moment, if the judgment of the next step is carried out according to the first judgment mode, the online monitoring unit carries out judgment.
If the pH value is detected as: the pH is less than 8.8 or more than 9.2, which indicates that the result information of the repair liquid is not in the threshold value, at the moment, the online monitoring unit directly feeds back an abnormal signal to the control unit, and the control unit does not send a control system starting signal and simultaneously sends an alarm signal to remind an operator to check or replace the repair liquid.
Such as a threshold range of TDS values of distilled water < 50. In the detection result information of the repair liquid, the TDS value is detected to be 100, which indicates that the result information of the repair liquid is within the threshold value, and at this time, if the online monitoring unit performs the judgment of the next step according to the first judgment mode.
If the TDS value is detected to be 10, the result information of the repair liquid is not in the threshold value, at the moment, the online monitoring unit directly feeds back an abnormal signal to the control unit, and the control unit does not send a control system starting signal and sends an alarm signal to remind an operator to check or replace the repair liquid.
If a second judgment mode is adopted, all the sensors transmit the detection result information of all the reagents to the online monitoring unit, and in the judgment process of the online monitoring unit, if the detection result information of one reagent is not within the corresponding threshold value, the online monitoring unit directly interrupts the judgment, feeds back an abnormal signal to the control unit, and simultaneously sends an alarm signal to remind an operator to check or replace the corresponding reagent.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. A method for reducing abnormal staining caused by human error by a pathological staining system is characterized by comprising the following steps:
respectively determining corresponding normal range threshold values according to the types of reagents required in the dyeing process, and storing the normal range threshold values;
a control unit of the pathological staining system receives a wake-up signal for starting the system, and the control unit sends a starting signal to an online monitoring control unit;
after the on-line monitoring unit receives the starting signal, the on-line monitoring unit controls the corresponding sensor to start, and the corresponding sensor transmits the detection result information of the corresponding reagent to the on-line monitoring unit;
the online monitoring unit compares the received detection result information with the threshold value of the corresponding type of reagent, and judges that:
if the detection result information is within the threshold range of the corresponding type of reagent, the online monitoring unit feeds back a normal signal to the control unit, and the control unit controls the pathological staining system to start;
if the detection result information is not in the threshold range of the corresponding type of reagent, the online monitoring unit feeds back an abnormal signal to the control unit, and the control unit does not start the pathological staining system and sends a warning signal after receiving the abnormal signal;
the on-line monitoring unit compares the received detection result information with the threshold value of the corresponding type of reagent, and comprises the following steps:
determining the type N of the reagent, wherein N is a natural number;
1) the online monitoring unit judges the detection result information of the first reagent and the threshold value of the first reagent; if the detection result information of the first reagent is within the threshold range of the first reagent, the online monitoring unit carries out the next step;
if the detection result information of the first reagent is not in the threshold range of the first reagent, the online monitoring unit feeds back an abnormal signal to the control unit;
2) the online monitoring unit judges the detection result information of the second reagent and the threshold value of the second reagent;
if the detection result information of the second reagent is within the threshold range of the second reagent, the online monitoring unit carries out the next step;
if the detection result information of the second reagent is not in the threshold range of the second reagent, the online monitoring unit feeds back an abnormal signal to the control unit;
3) until the online monitoring unit judges the detection result information of the Nth reagent and the threshold value of the Nth reagent;
if the detection result information of the Nth reagent is within the threshold range of the Nth reagent, the online monitoring unit feeds back a normal signal to the control unit, and the control unit controls the pathological staining system to start;
if the detection result information of the Nth reagent is not in the threshold range of the Nth reagent, the online monitoring unit feeds back an abnormal signal to the control unit;
the detection result information of each reagent comprises pH value result information, and the threshold value range of the pH value of each reagent is the same or different;
each of the reagent test result information includes TDS value result information, and a threshold range of TDS values of each of the reagents is the same or different.
2. The pathological staining system of claim 1, wherein the on-line monitoring unit compares the received detection result information with a threshold value of a corresponding kind of reagent, comprising the steps of:
determining the type N of the reagent, wherein N is a natural number;
the online monitoring unit receives the detection result information of the N reagents, compares the detection result information with the threshold values of the corresponding reagents respectively, and judges that:
if the detection result information of the N reagents is respectively in the corresponding reagent threshold value range, the online monitoring unit feeds back a normal signal to the control unit;
and if the detection result information of one reagent is not in the corresponding reagent threshold range, the online monitoring unit feeds back an abnormal signal to the control unit.
3. The pathological staining system of claim 1, wherein the reagent comprises a plurality of detection assisting reagents, and the pH of the detection assisting reagents has a threshold range of pH:
(1) the pH value range of the acidic repair liquid is as follows: 6.0 plus or minus 0.2;
(2) the pH value range of the neutral repair liquid is as follows: 7.4 plus or minus 0.2;
(3) the pH value range of the alkaline repair liquid is as follows: 8.0 plus or minus 0.2 to 9.0 plus or minus 0.2;
(4) PBS buffer pH range: 7.4 plus or minus 0.2;
(5) distilled water pH range: 7.0 +/-0.5.
4. The pathological staining system of claim 1 wherein the threshold range of TDS values is:
(1) the TDS of the acidic repair liquid is less than 1000;
(2) the TDS of the neutral repair liquid is less than 500;
(3) the TDS of the alkaline repair liquid is less than 200;
(4) TDS of PBS buffer solution is less than 6500;
(5) the TDS of the distilled water is less than 50.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910447746.XA CN110132695B (en) | 2019-05-27 | 2019-05-27 | Method for reducing abnormal dyeing caused by human error by pathological dyeing system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910447746.XA CN110132695B (en) | 2019-05-27 | 2019-05-27 | Method for reducing abnormal dyeing caused by human error by pathological dyeing system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110132695A CN110132695A (en) | 2019-08-16 |
| CN110132695B true CN110132695B (en) | 2022-01-11 |
Family
ID=67582038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910447746.XA Active CN110132695B (en) | 2019-05-27 | 2019-05-27 | Method for reducing abnormal dyeing caused by human error by pathological dyeing system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110132695B (en) |
Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001022052A1 (en) * | 1999-09-23 | 2001-03-29 | Aurel Popa | Device assembly for preparing and analyzing tissue for microscopic examinations |
| CN101221101A (en) * | 2002-06-20 | 2008-07-16 | 视觉生物体系有限公司 | Biological reaction apparatus with draining mechanism |
| CN103119451A (en) * | 2010-07-23 | 2013-05-22 | 贝克曼考尔特公司 | System and method including analytical units |
| CN103175724A (en) * | 2013-03-20 | 2013-06-26 | 嘉兴凯实生物科技有限公司 | Full-automatic dyeing machine |
| CN203572706U (en) * | 2013-10-10 | 2014-04-30 | 广州安必平自动化检测设备有限公司 | Photoelectric detection mechanism for cell staining device |
| CN203798653U (en) * | 2014-04-28 | 2014-08-27 | 深圳迈瑞生物医疗电子股份有限公司 | Dyeing box transportation device and detection mechanism |
| CN203837996U (en) * | 2014-04-30 | 2014-09-17 | 浙江世纪康大医疗科技有限公司 | Monitoring mechanism for reagent use |
| CN104272083A (en) * | 2012-02-24 | 2015-01-07 | 英士查诺尔有限公司 | System, apparatuses and devices for pretreating cells |
| CN105571925A (en) * | 2016-03-09 | 2016-05-11 | 福州迈新生物技术开发有限公司 | Dyeing module applied to biological samples on glass slides and dyeing method thereof |
| CN106053188A (en) * | 2016-06-24 | 2016-10-26 | 广东医学院 | Gel dyeing-decoloration integrated equipment and control method thereof |
| CN205719714U (en) * | 2016-06-25 | 2016-11-23 | 南京健邦锦源医疗仪器有限公司 | A kind of cell separation film-making dyeing all-in-one |
| CN206038393U (en) * | 2016-08-17 | 2017-03-22 | 广州三瑞医疗器械有限公司 | Cell dyeing pelleter |
| CN107102159A (en) * | 2017-04-27 | 2017-08-29 | 安徽工程大学 | For Multi-example water quality while the self-reacting device and its detection method of on-line checking |
| CN207502481U (en) * | 2017-11-01 | 2018-06-15 | 石家庄给源环保科技有限公司 | Portable water-quality analysis instrument |
| CN207585989U (en) * | 2017-11-30 | 2018-07-06 | 珠海贝索生物技术有限公司 | A kind of automatic staining device |
| WO2019014234A1 (en) * | 2017-07-11 | 2019-01-17 | Sanford Burnham Prebys Medical Discovery Institute | Nucleoporins as drug targets for anti-proliferative therapeutics |
| CN109238812A (en) * | 2018-10-26 | 2019-01-18 | 天津源天晟科技发展有限公司 | A kind of automatic staining device and its control method |
| CN109342156A (en) * | 2018-10-29 | 2019-02-15 | 重庆英特力科技有限公司 | Automatic dye sheet devices |
| CN109781747A (en) * | 2019-03-05 | 2019-05-21 | 齐鲁工业大学 | Cytologic specimen print dyeing effect detection method and system based on machine vision |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2006202931A1 (en) * | 2001-03-07 | 2006-08-03 | Ventana Medical Systems, Inc. | Automated immunohistochemical and in situ hybridization assay formulations |
| US8153010B2 (en) * | 2009-01-12 | 2012-04-10 | American Air Liquide, Inc. | Method to inhibit scale formation in cooling circuits using carbon dioxide |
| US9086383B2 (en) * | 2012-09-14 | 2015-07-21 | Halliburton Energy Services, Inc. | Systems and methods for monitoring chemical processes |
| CN105980852B (en) * | 2014-04-17 | 2019-07-26 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analysis method and system, device |
| JP6139603B2 (en) * | 2015-06-30 | 2017-05-31 | シスメックス株式会社 | Smear preparation apparatus, blood sample processing system, and method for cleaning a blood processing section in a smear preparation apparatus |
| CN107367607A (en) * | 2017-05-25 | 2017-11-21 | 长沙金域医学检验所有限公司 | A kind of Pathologic specimen section SABC operating method and its system |
-
2019
- 2019-05-27 CN CN201910447746.XA patent/CN110132695B/en active Active
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001022052A1 (en) * | 1999-09-23 | 2001-03-29 | Aurel Popa | Device assembly for preparing and analyzing tissue for microscopic examinations |
| CN101221101A (en) * | 2002-06-20 | 2008-07-16 | 视觉生物体系有限公司 | Biological reaction apparatus with draining mechanism |
| CN103119451A (en) * | 2010-07-23 | 2013-05-22 | 贝克曼考尔特公司 | System and method including analytical units |
| CN104272083A (en) * | 2012-02-24 | 2015-01-07 | 英士查诺尔有限公司 | System, apparatuses and devices for pretreating cells |
| CN103175724A (en) * | 2013-03-20 | 2013-06-26 | 嘉兴凯实生物科技有限公司 | Full-automatic dyeing machine |
| CN203572706U (en) * | 2013-10-10 | 2014-04-30 | 广州安必平自动化检测设备有限公司 | Photoelectric detection mechanism for cell staining device |
| CN203798653U (en) * | 2014-04-28 | 2014-08-27 | 深圳迈瑞生物医疗电子股份有限公司 | Dyeing box transportation device and detection mechanism |
| CN203837996U (en) * | 2014-04-30 | 2014-09-17 | 浙江世纪康大医疗科技有限公司 | Monitoring mechanism for reagent use |
| CN105571925A (en) * | 2016-03-09 | 2016-05-11 | 福州迈新生物技术开发有限公司 | Dyeing module applied to biological samples on glass slides and dyeing method thereof |
| CN106053188A (en) * | 2016-06-24 | 2016-10-26 | 广东医学院 | Gel dyeing-decoloration integrated equipment and control method thereof |
| CN205719714U (en) * | 2016-06-25 | 2016-11-23 | 南京健邦锦源医疗仪器有限公司 | A kind of cell separation film-making dyeing all-in-one |
| CN206038393U (en) * | 2016-08-17 | 2017-03-22 | 广州三瑞医疗器械有限公司 | Cell dyeing pelleter |
| CN107102159A (en) * | 2017-04-27 | 2017-08-29 | 安徽工程大学 | For Multi-example water quality while the self-reacting device and its detection method of on-line checking |
| WO2019014234A1 (en) * | 2017-07-11 | 2019-01-17 | Sanford Burnham Prebys Medical Discovery Institute | Nucleoporins as drug targets for anti-proliferative therapeutics |
| CN207502481U (en) * | 2017-11-01 | 2018-06-15 | 石家庄给源环保科技有限公司 | Portable water-quality analysis instrument |
| CN207585989U (en) * | 2017-11-30 | 2018-07-06 | 珠海贝索生物技术有限公司 | A kind of automatic staining device |
| CN109238812A (en) * | 2018-10-26 | 2019-01-18 | 天津源天晟科技发展有限公司 | A kind of automatic staining device and its control method |
| CN109342156A (en) * | 2018-10-29 | 2019-02-15 | 重庆英特力科技有限公司 | Automatic dye sheet devices |
| CN109781747A (en) * | 2019-03-05 | 2019-05-21 | 齐鲁工业大学 | Cytologic specimen print dyeing effect detection method and system based on machine vision |
Non-Patent Citations (1)
| Title |
|---|
| 谈免疫组化技术标准化;张英哲;《中国农村卫生》;20140415;正文第1-2节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110132695A (en) | 2019-08-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nabity et al. | Urinary biomarkers of renal disease in dogs with X‐linked hereditary nephropathy | |
| CN104075931B (en) | A kind of protein example rapid preprocessing method in situ | |
| CN102667492B (en) | Automatic analysis device and method for executing process of analysis preparation in automatic analysis device | |
| CN102255774A (en) | Test system and test method for switch-on and switch-off of communication device as well as fault location method | |
| Quesada et al. | Urinary aminopeptidase activities as early and predictive biomarkers of renal dysfunction in cisplatin-treated rats | |
| CN104755935B (en) | The prognosis of cancer and diagnostic method | |
| CN105083168A (en) | Control and diagnosis of a controller wake up feature | |
| JP2017502266A (en) | Assay device | |
| CN108037298A (en) | Automatic analyzer and its wake-up control method and control system | |
| WO2023098804A1 (en) | Use of urinary protein marker in diagnosis of hereditary angioedema | |
| CN110132695B (en) | Method for reducing abnormal dyeing caused by human error by pathological dyeing system | |
| EP1437591B1 (en) | Method for diagnosing kidney diseases | |
| CN113965483A (en) | Device and method for testing time from auto TOSAR network management pre-dormancy to dormancy | |
| Porcel et al. | Comparison of pleural N‐terminal pro‐B‐type natriuretic peptide, midregion pro‐atrial natriuretic peptide and mid‐region pro‐adrenomedullin for the diagnosis of pleural effusions associated with cardiac failure | |
| CN109901481B (en) | Data acquisition and storage system and method for intelligent multichannel digital signals | |
| Craven et al. | [4] Use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis | |
| CN113588739A (en) | Continuous arterial blood detection system | |
| CN204359796U (en) | A kind of test strips detecting ECP and MPO | |
| CN108802380A (en) | Colloid gold test paper detecting system and detection method | |
| CN104826677B (en) | A kind of immune detection micro-fluidic chip for acute abdomen disease investigation and preparation method thereof and detection method | |
| CN114609382A (en) | System for identifying immunoassay and application method thereof | |
| CN106226390A (en) | A kind of breast milk composition analyser | |
| CN117250346A (en) | Method for quality control of sample antigen detection through internal reference protein | |
| CN114152760B (en) | beta-HCG concentration detection system and method | |
| CN109508257A (en) | A kind of DC test method and system based on UTC |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Building 12, innovation park, Haixi high tech Industrial Park, Fuzhou high tech Zone, No. 3, Keji East Road, Minhou County, Fuzhou City, Fujian Province Patentee after: FUZHOU MAIXIN BIOTECH, Co.,Ltd. Patentee after: Lumingte biological system Co., Ltd Address before: 350000 building 12, innovation park, Haixi high tech Industrial Park, Fuzhou high tech Zone, No. 3, Keji East Road, Minhou County, Fuzhou City, Fujian Province Patentee before: FUZHOU MAIXIN BIOTECH, Co.,Ltd. Patentee before: Lutes biosystems |