CN108802380A - Colloid gold test paper detecting system and detection method - Google Patents
Colloid gold test paper detecting system and detection method Download PDFInfo
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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Abstract
The present invention discloses a kind of colloid gold test paper detecting system, including sequentially connected colloid gold test paper detection device, communication module and remote server；The colloid gold test paper detection device includes sequentially connected test paper slot, detection switch, data acquisition module, microprocessor and the host computer for being provided with socket end, and the microprocessor is connect by communication module with remote server.The present invention carries out automatic detection by using colloidal gold detection device to colloid gold test paper, and remote management is realized to testing result by communication module and remote server, thus avoiding, because while artificial subjective factor is judged by accident, conveniently remotely monitoring and managing.
Description
Technical field
The present invention relates to colloidal gold detection fields, and in particular to colloid gold test paper detecting system and detection method.
Background technology
Colloidal gold strip is a kind of quick and easy detection technique being prepared based on colloidal gold immunity chromatography, by
It is easy to operate in its, detection time is short, can Site Detection the advantages that, therefore applied to medical treatment and food security is led by extensive
Domain.It is micro- that colloidal gold strip can be used for medical diagnosis on disease, domestic medicine, agricultural and veterinary chemicals residue detection, illegal addition analyte detection and cause of disease
Biological detection etc., convenient and efficient.
Currently, quickly detection relies primarily on range estimation, that is, eye-observation, artificial reading, such mode is used to be easy due to master
The influence of sight factor is judged by accident, and preserves inconvenience for the archive for reading result, is unfavorable for medical treatment and public health shelves
The foundation of case.Therefore, colloidal gold strip can be completed and detect automatically by developing one kind, and can realize remote management to testing result
Detecting system is of great significance.
Invention content
In order to overcome the deficiencies of the prior art, a kind of colloid gold test paper detecting system of the application offer and detection method, lead to
It crosses and automatic detection is carried out to colloid gold test paper using colloidal gold detection device, and connected by communication module and remote server
It connects, the remote management to testing result can be not only realized while avoiding because artificial subjective factor is judged by accident, remotely
Server can also realize long-range monitoring at any time from colloid gold test paper detection device called data.The present invention passes through following technology
Scheme is realized：
Colloid gold test paper detecting system, including sequentially connected colloid gold test paper detection device, communication module and long-range clothes
Business device；The colloid gold test paper detection device includes the sequentially connected test paper slot for being provided with socket end, detection switch, data
Acquisition module, microprocessor and host computer, the microprocessor are connect by communication module with remote server.
Further, further include the mobile terminal being connect with communication module.
Further, the colloid gold test paper detection device is provided with the fault detection mechanism being connect with communication module, institute
It states remote server and is provided with fault message receiving mechanism.
Further, the test paper slot is provided with multiple test paper channels independent of each other, the setting of each test paper channel
There are one socket ends.
Further, the data acquisition module of the colloid gold test paper detection device includes multiple color sensors, described
The quantity of color sensor is equal with the test paper number of channels of test paper slot, and each test paper of the test paper slot is logical
Road is connect with a color sensor respectively by detection switch.
Further, it is provided with color card in the color sensor.
Further, the colloid gold test paper detection device further includes the LCD display module being connect respectively with microprocessor
And voice broadcast module.
A kind of detection method is used to detect colloid gold test paper to be measured by above-mentioned colloid gold test paper detecting system, including：
Step S1：Detect whether that test paper enters test paper slot by detection switch, if so, step S2 is carried out, if
Nothing controls the detection device and maintains or enter dormant state；
Step S2：It controls the detection device and enters working condition；
Step S3：It controls the data acquisition module and obtains data, and by the data transmission got to microprocessor；
Step S4：The data that the microprocessor receives are handled and stored, whether detection data stores success,
If stored successfully, step S5 is carried out, otherwise, carries out step S3；
Step S5：By the data transmission after microprocessor processes to host computer, by host computer to the data that receive into
Row is handled and is stored, and whether detection data stores success, if stored successfully, step S6 is carried out, otherwise, to the data received
It is handled and is stored again；Meanwhile by the data transmission after microprocessor processes to communication module, communication module is to receiving
Data carry out protocol packing after send remote server to；
Step S6：It controls the detection device and enters dormant state until there is new test paper to enter test paper slot.
Further, step S5 further includes sending the data after microprocessor processes to movement eventually by communication module
End.
Further, it is logical to be provided with multiple test paper independent of each other for the test paper slot of the colloid gold test paper detection device
Road, there are one socket ends for the setting of each test paper channel, and there are one color sensor, the colors for the connection of each test paper channel
Color card is provided in sensor, step S3 is specifically included：
Step S301：The test paper channel of test paper entrance is tested with by detection switch；
Step S302：It controls and all enter data with the color sensors that are connected of test paper channel for thering is test paper to enter and adopt
Collection state, wherein the next work step of each color sensor includes：
Step S303：Obtain the time point that test paper enters test paper slot；
Step S304：Detection such as has C lines and T lines simultaneously into the test paper of test paper slot, is walked after carrying out step S305
Rapid S308, such as only C lines or T lines carry out step S308 after carrying out step S306, such as both without C lines or without T lines, carry out step
Step S6 is carried out after S307；
Step S305：Obtain the relative color value of T lines and C lines；
Step S306：According to color card, the color value of C lines or T lines is obtained；
Step S307：Judge that test paper is invalid；
Step S308：Send the time point of acquisition and color value to microprocessor.
The present invention is a kind of intelligent checking system, is carried out to colloid gold test paper by using colloidal gold detection device automatic
Change detection, and remote management is realized to testing result by communication module and remote server, it can be to avoid because artificial subjective
There is the problem of judging by accident or achieving not in time in factor, and remote server is applied not only to receive the number of colloidal gold detection device transmission
According to, it can also be as needed at any time from called data in the colloidal gold detection device of working condition or dormant state, realization is long-range
Monitoring, convenient for management；By mobile terminal, user can understand detection progress and testing result at any time, flexibly and easily, when reduction
Between cost；Using color sensor as data acquisition module, detection speed is fast, adapts to wavelength automatically, is capable of detecting when between color
Small differences, and unrelated with the background color of colloid gold test paper, accuracy rate is high；In addition, the present invention by be arranged it is multiple that
This independent test paper channel, may be implemented multiple test paper while detecting, to greatly improve working efficiency.Control provided by the invention
Method processed can make detection device enter dormant state in not Test paper, not only energy conservation and environmental protection, it is possibility to have effect extends inspection
The service life of measurement equipment.
Description of the drawings
Fig. 1 is the structure diagram of colloid gold test paper detecting system.
Fig. 2 is the detection method flow chart of colloid gold test paper detecting system.
Fig. 3 is the control method flow chart of color sensor.
Specific implementation mode
It is below in conjunction with the accompanying drawings and specific real in order to make those skilled in the art more fully understand technical scheme of the present invention
Applying example, the present invention is described in further detail.
Embodiment 1
As shown in Figure 1, the present embodiment provides a kind of colloid gold test paper detecting system, including sequentially connected colloid gold test paper
Detection device, communication module and remote server；Colloid gold test paper detection device includes sequentially connected being provided with socket end
Test paper slot, detection switch, data acquisition module, microprocessor and host computer, microprocessor pass through communication module and long-range clothes
Business device connection.In addition, the present embodiment can also include the mobile terminal being connect with communication module, here, mobile terminal can be
Cell phone application.
In order to keep the present embodiment safe and reliable at work, fault testing mechanism can be arranged in colloid gold test paper detection device
Structure, and fault detection mechanism is made to be connect with communication module, meanwhile, fault message receiving mechanism is accordingly arranged in remote server.This
Sample can detect the working condition or operating status of colloid gold test paper detection device by fault detection mechanism at any time, once hair
It is raw abnormal, on the one hand, colloid gold test paper detection device stops detecting up to fault clearance, on the other hand, colloid gold test paper detection
Exception information is sent to the fault message receiving mechanism of remote server by equipment by communication module, keeps remote server timely
Cut-out obtains the information channel of test paper detection data, to avoid receiving error message.
In the present embodiment, data acquisition module can be the color sensor for being internally provided with color card, and color is arranged
Sample can provide color contrast in the case where colloid gold test paper to be detected is without reference to color line, color is facilitated to sense
Device obtains the color value of colloid gold test paper to be detected.
Detection data is extracted in order to facilitate staff or user, data output end can be arranged in microprocessor, by this
Data output end can obtain the detection data of the colloid gold test paper stored in microprocessor at any time.
In addition, the LCD display module and voice broadcast module being connect with microprocessor can also be arranged in the present embodiment, to inspection
The detection progress and detection data of colloid gold test paper in survey carry out real-time display and report, user-friendly.
When implementing the present embodiment, LCD display module and voice broadcast module can both be used for showing or prompting detection progress,
It can also be used to show or prompt testing result；Remote server is applied not only to receive the data of colloidal gold detection device transmission,
Long-range prison can also be realized as needed at any time from called data in the colloidal gold detection device of working condition or dormant state
It surveys, convenient for management；By mobile terminal or cell phone application, user can understand detection progress and testing result at any time, flexibly side
Just, time cost is reduced.
Embodiment 2
On the basis of embodiment 1, in colloid gold test paper detecting system provided in this embodiment, colloidal gold detection device
Test paper slot is provided with multiple test paper channels independent of each other, and there are one socket ends for the setting of each test paper channel, in addition, data
Acquisition module includes multiple color sensors, and the quantity of color sensor is equal with the test paper number of channels of test paper slot, and is tried
Each test paper channel of paper slot is connect with a color sensor respectively by detection switch.
When implementing the present embodiment, user can be put into one in multiple or all test paper channel of test paper slot simultaneously
Colloid gold test paper to be detected improves working efficiency to realize while detect multiple colloid gold test papers.
Embodiment 3
As shown in Fig. 2, the present embodiment provides a kind of detection method, the glue for being provided by embodiment 1 or embodiment 2
Body gold test paper detecting system detects colloid gold test paper to be measured, including：
Step S1：Detect whether that test paper enters test paper slot by detection switch, if so, step S2 is carried out, if
Nothing controls the detection device and maintains or enter dormant state；
Step S2：It controls the detection device and enters working condition；
Step S3：It controls the data acquisition module and obtains data, and by the data transmission got to microprocessor；
Step S4：The data that the microprocessor receives are handled and stored, whether detection data stores success,
If stored successfully, step S5 is carried out, otherwise, carries out step S3；
Step S5：By the data transmission after microprocessor processes to host computer, by host computer to the data that receive into
Row is handled and is stored, and whether detection data stores success, if stored successfully, step S6 is carried out, otherwise, to the data received
It is handled and is stored again；Meanwhile by the data transmission after microprocessor processes to communication module, communication module is to receiving
Data carry out protocol packing after send remote server to；Meanwhile the data after microprocessor processes are passed through into communication module
Send mobile terminal to.
Step S6：It controls the detection device and enters dormant state until there is new test paper to enter test paper slot.
In this control method, the data that microprocessor receives are handled, may include to receive data with it is pre-
The threshold first set is calculated, and judges test result according to result of calculation to be negative or positive, then the original to receiving again
Beginning data and result of calculation are stored, or are only stored to result of calculation；Microprocessor sends the data of host computer to
May include the data after the initial data and/or microprocessor processes that acquisition module transmits；By host computer to receiving
Data are handled, and may include to the data after the microprocessor processes that receive carrying out taxonomic revision, or to receiving
The initial data of acquisition module transmission is calculated again, if result of calculation is consistent with the result of calculation of microprocessor, is pressed
Result of calculation stores, and if any difference, is then detected from newly, to improve test accuracy rate.
In addition, remote server can not only be handled and stored to the data of reception, it can also as needed at any time
From called data in the colloid gold test paper detection device of working condition or dormant state, to realize Distant supervision and control.It is logical
Mobile terminal is crossed, user is facilitated to understand detection case at any time, flexibility and reliability can reduce time cost.
As shown in figure 3, when the data acquisition module of colloid gold test paper detection device is the interior color for being provided with color card
When sensor, step S3 is specifically included：
Step S301：Obtain the time point that test paper enters test paper slot；
Step S302：Detection such as has C lines and T lines simultaneously into the test paper of test paper slot, is walked after carrying out step S303
Rapid S306, such as only C lines or T lines carry out step S306 after carrying out step S304, such as both without C lines or without T lines, carry out step
Step S6 is carried out after S305；
Step S303：Obtain the relative color value of T lines and C lines；
Step S304：According to color card, the color value of C lines or T lines is obtained；
Step S305：Judge that test paper is invalid；
Step S306：Send the time point of acquisition and color value to microprocessor.
When the test paper slot of colloid gold test paper detection device is provided with multiple test paper channels independent of each other, each test paper
There are one socket ends for channel setting, and there are one the color sensors for being internally provided with color card for the connection of each test paper channel
When, step S3 is specifically included：
Step S301：The test paper channel of test paper entrance is tested with by detection switch；
Step S302：It controls and all enter data with the color sensors that are connected of test paper channel for thering is test paper to enter and adopt
Collection state, wherein as shown in figure 3, the next work step of each color sensor includes：
Step S303：Obtain the time point that test paper enters test paper slot；
Step S304：Detection such as has C lines and T lines simultaneously into the test paper of test paper slot, is walked after carrying out step S305
Rapid S308, such as only C lines or T lines carry out step S308 after carrying out step S306, such as both without C lines or without T lines, carry out step
Step S6 is carried out after S307；
Step S305：Obtain the relative color value of T lines and C lines；
Step S306：According to color card, the color value of C lines or T lines is obtained；
Step S307：Judge that test paper is invalid；
Step S308：Send the time point of acquisition and color value to microprocessor.
When the present embodiment is embodied, it can also be carried out before carrying out step S1：Glue is detected by fault detection mechanism
Body gold test paper detection device, such as fault-free carry out step S1, such as faulty, and fault message is sent to far by communication module
Journey server, remote server, which sends out fault cues signal and cuts off from colloid gold test paper detection device, obtains test paper detection data
Information channel, until carrying out step S1 after fault clearance.
What needs to be explained here is that the detection device that embodiment 1 or embodiment 2 provide can not only be used for detecting colloidal gold
The C lines of test paper and/or the color value of T lines can also be used to the background color value of detection colloid gold test paper.When specific detection, root
According to the type of colloid gold test paper to be detected, corresponding detection is made.
Provide the detection sample of 3 specific colloidal gold strips again below：
Sample 1：
Test strips：ICAM-1 is the colloidal gold strip of Testing index, stuck type.
Sample：0.01M PBS
Process：80 microlitres of 0.01M PBS are added dropwise to the well of colloidal gold strip, entire test strips are inserted into glue later
In the test paper slot of body gold detection device, after test strips are inserted into equipment, equipment passes through voice broadcast mould automatically into working condition
Block and LCD show display module prompt " Test paper ", and a period of time (user is set as needed, such as 10 minutes), voice was broadcast afterwards
Module and LCD display module prompt " data upload " are reported, voice broadcast module and LCD display module prompt " inspection after a period of time
Survey finishes ", then equipment is automatically into dormant state.At this point, showing that T line grades be 1, C line grades are 5 on the APP of mobile phone.It pulls out
Go out test strips, it is royal purple to visually observe detection card C lines, and T lines are shaping band.
Sample 2：
Test strips：ICAM-1 is the colloidal gold strip of Testing index, stuck type.
Sample：With the ICAM-1 recombinant protein solution of 0.01M PBS dissolvings, a concentration of 2ng/ml blocks minimum inspection for detection
Rising limit.
Process：80 microlitres of 0.01M PBS are added dropwise to the well of colloidal gold strip, entire test strips are inserted into glue later
In the test paper slot of body gold detection device, after test strips are inserted into equipment, equipment Auto Power On, voice broadcast module and LCD are shown
Module prompts " Test paper ", voice broadcast module and LCD display module prompt " data upload ", a period of time after a period of time
Voice broadcast module and LCD display module prompt " detection finishes " afterwards, then equipment enters dormant state.The APP of mobile phone at this time
It is 5 that upper display T line grades, which are 2, C line grades,.Detection card is extracted, it is royal purple to visually observe detection card C lines, and T lines are light red
Color.
Sample 3：
Test strips：ICAM-1 is the colloidal gold strip of Testing index, stuck type.
Sample：With the ICAM-1 recombinant protein solution of 0.01M PBS dissolvings, a concentration of 100ng/ml.
Process：80 microlitres of 0.01M PBS are added dropwise to the well of colloidal gold strip, later by entire colloidal gold strip
It is inserted into the test paper slot of colloidal gold detection device, after test strips are inserted into equipment, equipment enters working condition, voice broadcast module
And LCD display module prompt " Test paper ", voice broadcast module and LCD display module prompt are " in data after a period of time
Biography ", voice broadcast module and LCD display module prompt " detection finishes " after a period of time, then equipment is automatically into suspend mode shape
State.Show that T line grades be 4, C line grades are 5 on the APP of mobile phone at this time.Detection card is extracted, it is deep to visually observe detection card C lines
Aubergine, T lines are aubergine, and T lines are deeper than the T line colors in embodiment 2.
It is shown when it is implemented, the display in mobile terminal or cell phone application could be provided as grade, may be set to be the moon
Property or positive findings are shown.
It the above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
The limitation of the present invention, protection scope of the present invention should be subject to claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
Protection scope of the present invention is also should be regarded as into retouching.
Claims (10)
1. colloid gold test paper detecting system, which is characterized in that including sequentially connected colloid gold test paper detection device, communication module
And remote server；The colloid gold test paper detection device includes the sequentially connected test paper slot for being provided with socket end, detection
Switch, data acquisition module, microprocessor and host computer, the microprocessor are connect by communication module with remote server.
2. colloid gold test paper detecting system according to claim 1, which is characterized in that further include being connect with communication module
Mobile terminal.
3. colloid gold test paper detecting system according to claim 1, which is characterized in that the colloid gold test paper detection device
It is provided with the fault detection mechanism being connect with communication module, the remote server is provided with fault message receiving mechanism.
4. colloid gold test paper detecting system according to claim 1, which is characterized in that the test paper slot is provided with multiple
Test paper channel independent of each other, there are one socket ends for the setting of each test paper channel.
5. colloid gold test paper detecting system according to claim 4, which is characterized in that the colloid gold test paper detection device
Data acquisition module include multiple color sensors, the test paper channel of the quantity of the color sensor and the test paper slot
Quantity is equal, and each test paper channel of the test paper slot is connect with a color sensor respectively by detection switch.
6. the colloid gold test paper detecting system according to claim 3-5 any one, which is characterized in that the color passes
Color card is provided in sensor.
7. colloid gold test paper detection device according to claim 1, which is characterized in that the colloid gold test paper detection device
Further include the LCD display module and voice broadcast module being connect respectively with microprocessor.
8. a kind of detection method is tried for detecting colloidal gold to be measured by colloid gold test paper detecting system described in claim 1
Paper, which is characterized in that including：
Step S1：Detect whether that test paper enters test paper slot by detection switch, if so, step S2 is carried out, if nothing, control
It makes the detection device and maintains or enter dormant state；
Step S2：It controls the detection device and enters working condition；
Step S3：It controls the data acquisition module and obtains data, and by the data transmission got to microprocessor；
Step S4：The data that the microprocessor receives are handled and stored, whether detection data stores success, if
It stores successfully, carries out step S5, otherwise, carry out step S3；
Step S5：By the data transmission after microprocessor processes to host computer, by host computer at the data that receive
It manages and stores, whether detection data stores success, if stored successfully, carries out step S6, otherwise, again to the data that receive
It is handled and is stored；Meanwhile by the data transmission after microprocessor processes to communication module, communication module is to the number that receives
According to carry out protocol packing after send remote server to；
Step S6：It controls the detection device and enters dormant state until there is new test paper to enter test paper slot.
9. a kind of detection method according to claim 8, which is characterized in that step S5 further includes will be after microprocessor processes
Data mobile terminal is sent to by communication module.
10. a kind of detection method according to claim 8, which is characterized in that the examination of the colloid gold test paper detection device
Paper slot is provided with multiple test paper channels independent of each other, and there are one socket end, each test paper for the setting of each test paper channel
Channel connection is provided with color card, step S3 is specifically included there are one color sensor in the color sensor：
Step S301：The test paper channel of test paper entrance is tested with by detection switch；
Step S302：It controls all color sensors being connected with the test paper channel for thering is test paper to enter and enters data acquisition shape
State, wherein the next work step of each color sensor includes：
Step S303：Obtain the time point that test paper enters test paper slot；
Step S304：Detection such as has C lines and T lines simultaneously into the test paper of test paper slot, and step is carried out after carrying out step S305
S308, such as only C lines or T lines carry out step S308 after carrying out step S306, such as both without C lines or without T lines, carry out step S307
Step S6 is carried out afterwards；
Step S305：Obtain the relative color value of T lines and C lines；
Step S306：According to color card, the color value of C lines or T lines is obtained；
Step S307：Judge that test paper is invalid；
Step S308：Send the time point of acquisition and color value to microprocessor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810994733.XA CN108802380A (en) | 2018-08-29 | 2018-08-29 | Colloid gold test paper detecting system and detection method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810994733.XA CN108802380A (en) | 2018-08-29 | 2018-08-29 | Colloid gold test paper detecting system and detection method |
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| CN108802380A true CN108802380A (en) | 2018-11-13 |
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|---|---|---|---|
| CN201810994733.XA Withdrawn CN108802380A (en) | 2018-08-29 | 2018-08-29 | Colloid gold test paper detecting system and detection method |
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| Country | Link |
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| CN (1) | CN108802380A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111751538A (en) * | 2020-06-10 | 2020-10-09 | 四川大学 | Colloidal gold test paper detecting system |
| CN112230574A (en) * | 2020-07-30 | 2021-01-15 | 深圳加美生物有限公司 | Low-power-consumption multi-channel colloidal gold detection instrument circuit |
-
2018
- 2018-08-29 CN CN201810994733.XA patent/CN108802380A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111751538A (en) * | 2020-06-10 | 2020-10-09 | 四川大学 | Colloidal gold test paper detecting system |
| CN112230574A (en) * | 2020-07-30 | 2021-01-15 | 深圳加美生物有限公司 | Low-power-consumption multi-channel colloidal gold detection instrument circuit |
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Application publication date: 20181113 |