CN103674663B - Flowing exhaust dyeing instrument - Google Patents
Flowing exhaust dyeing instrument Download PDFInfo
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- CN103674663B CN103674663B CN201310643854.7A CN201310643854A CN103674663B CN 103674663 B CN103674663 B CN 103674663B CN 201310643854 A CN201310643854 A CN 201310643854A CN 103674663 B CN103674663 B CN 103674663B
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Abstract
A kind of flowing exhaust dyeing instrument, comprising: micro-color pond, for depositing the slide being loaded with sample of dyeing liquor and monolithic, having liquid injection port and leakage fluid dram; Liquid injection pipe, connects described liquid injection port and dyeing liquor container or washing fluid container; Discharging tube, connects described leakage fluid dram and dyeing instrument is outside; Peristaltic pump, is arranged on described liquid injection pipe, and its control end connects main control module; Light sensing apparatus, is arranged on micro-color pond side, for detecting the thickness of the sample that described slide is loaded with, and detection signal is supplied to main control module; Main control module, analyzes the detection signal of described sample thickness, controls dyeing time, and controls the operation of described peristaltic pump.The present invention can reduce reagent consumption; The light sensing apparatus arranged, can the thickness of automatic decision sample, and automatically changes required dyeing time by main control module; Utilize flow cell principle to come feed liquor and discharge opeing, eliminate the cross-contamination issue between sample, and the object of cleaning can be reached.
Description
Technical field
The present invention relates to the technical field of the dyeing of hematology, microbiology, pathology, cytology, reproductive medicine etc., be specifically related to a kind of exhaust dyeing instrument.
Background technology
Existing biology and medical sample dyeing equipment, comprise spray-painting and contaminate two large classes.Spray-painting is exactly the dyeing realizing sample to sample slide jet dyeing liquid by shower nozzle; Contaminate the slide being about to be loaded with sample to be placed in and to be equipped with in the staining bath of dyeing liquor, realize sample dyeing.
China's No. 200920140796.5 utility models disclose a kind of slide staining device, comprise shadoof and be connected at least one support of shadoof bottom, the upper and lower conducting of this support, its madial wall is fixed with at least two pieces of identical elongate support plate, the upper surface of these two pieces of back up pads is dispersed with uniformly the rectangle draw-in groove of symmetrical multiple support slides, the size of every two relative draw-in grooves, shape and the degree of depth are identical.
Above-mentioned dyeing apparatus is exhaust dyeing device, and its defect is:
1. dyeing liquor consumption, dyeing time need operate by hand, cause different people to operate the inconsistency produced, and take the defect of a large amount of labour; 2. be only applicable to batch dyeing, dyeing liquor consumption is large, and easily causes cross pollution, is therefore not suitable for all dyeing; 3., for sample its dyeing time restive of different-thickness, cause Color inconsistent, Color is not good.
Summary of the invention
The object of the invention is to provide a kind of flowing exhaust dyeing instrument, solves the existing manual deficiency contaminated, realizes robotization, standardization, conforming coloration requirements, both can ensure the quality requirements dyeed, also can reduce human cost.Object of the present invention is realized by following technical scheme:
A kind of flowing exhaust dyeing instrument, is characterized in that, comprising: micro-color pond, for depositing the slide being loaded with sample of dyeing liquor and monolithic, to have liquid injection port and leakage fluid dram; Liquid injection pipe, connects described liquid injection port and dyeing liquor container or washing fluid container; Discharging tube, connects described leakage fluid dram and dyeing instrument is outside; Peristaltic pump, is arranged on described liquid injection pipe, and its control end connects main control module; Dyeing liquor container, for storing dyeing liquor; Washing fluid container, for storing washing fluid; Light sensing apparatus, is arranged on micro-color pond side, for detecting the thickness of the sample that described slide is loaded with, and detection signal is supplied to main control module; Main control module, analyzes the detection signal of described sample thickness, controls dyeing time, and controls the operation of described peristaltic pump; Display and key-press module, its display section is for showing the running state information of described main control module, and its key part is used for described main control module input control signal.
As further technical scheme, described flowing exhaust dyeing instrument also comprises fluid level sensing apparatus, and be arranged on the warning liquid level place in micro-color pond, the sensing signal output terminal of this fluid level sensing apparatus connects described main control module; Described main control module also controls described peristaltic pump according to the sensing signal of fluid level sensing apparatus.
As further technical scheme, described flowing exhaust dyeing instrument also comprises overflow protection water jacket, and micro-color pond is arranged in this overflow protection water jacket, and overflow protection outer slot bottom is provided with spout hole.
As further technical scheme, described spout hole is connected with discharging tube, and this discharging tube is provided with solenoid valve.
As further technical scheme, described discharging tube is provided with peristaltic pump.
As concrete technical scheme, described liquid injection pipe, peristaltic pump and dyeing liquor container are more than two.
As concrete technical scheme, described light sensing apparatus is reflected light sensing apparatus, and its light transmitting terminal and optical receiving end are positioned at the same side of micro-color pond.
As concrete technical scheme, described light sensing apparatus is transmitted light sensing apparatus, and its light transmitting terminal and optical receiving end lay respectively at the both sides of micro-color pond.
As concrete technical scheme, the length of described micro-color pond × wide × thick is 55 ~ 60mm × 26 ~ 28mm × 3 ~ 5mm.
As concrete technical scheme, the liquid injection port be connected with described dyeing liquor container is arranged on the bottom of micro-color pond; The liquid injection port be connected with described washing fluid container is arranged on the side wall upper part of micro-color pond.
Beneficial effect of the present invention is: the cavity being reduced dip-dye by special structural design, to reduce reagent consumption; Light sensing apparatus is set, the sample of different-thickness, can its thickness of automatic decision, and automatically change required dyeing time by main control module; Utilize flow cell principle to come feed liquor and discharge opeing, eliminate the cross-contamination issue between sample, and the object of cleaning can be reached; The design of color pond milligram ammonia, then can reduce the cost of dyeing.
Accompanying drawing explanation
The organigram of the flowing exhaust dyeing instrument that Fig. 1 provides for the embodiment of the present invention.
The schematic diagram of the electric control system in the flowing exhaust dyeing instrument that Fig. 2 provides for the embodiment of the present invention.
Embodiment
As shown in Figure 1; the flowing exhaust dyeing instrument that the present embodiment provides, comprising: micro-color pond 11, liquid injection pipe 12, discharging tube 13, peristaltic pump 15, dyeing liquor container 16, washing fluid container 165, light sensing apparatus 17, fluid level sensing apparatus 18, main control module (composition graphs 2), display and key-press module (composition graphs 2), overflow protection water jacket 19 and solenoid valve 14.Wherein, liquid injection pipe 12, peristaltic pump 15, dyeing liquor container 16 are at least one group (being three groups) in Fig. 1.
Trace color pond 11, for depositing dyeing liquor, is loaded with the slide 20 of sample simultaneously for placing monolithic.Trace color pond 11 cavity volume is that 60mm(is long) x27mm(is wide) x3mm(is thick), its cavity wall top arranges the liquid injection port being used for washing fluid and entering, and bottom is provided with three liquid injection port and the leakage fluid dram for discharge opeing that enter for dyeing liquor.For can all be drained by liquid smoothly, cavity bottom is set to little inclined-plane, and leakage fluid dram designs in inclined-plane minimum point.
Liquid injection pipe 12 connects the liquid injection port of micro-color pond 11 and dyeing liquor container 16 and washing fluid container 165.Discharging tube 13 connects leakage fluid dram and the dyeing instrument outside of micro-color pond 11.Dyeing liquor container 16 supplies the dyeing liquor of micro-color pond 11 for storing, washing fluid container 165 supplies the washing fluid of micro-color pond 11 for storing.Peristaltic pump 15 is arranged on liquid injection pipe 12 and discharging tube 13, and its control end connects main control module, and controlled unlatching to carry dyeing liquor in micro-color pond 11, and dyeing liquor in the micro-color pond 11 of controlled discharge or washing fluid.Trace color pond 11 be arranged on overflow protection water jacket 19 in, overflow protection water jacket 19 for when there are special circumstances in micro-color pond 11, namely dyeing liquor or washing fluid excessive time carrying overflow dyeing liquor or washing fluid.Overflow protection water jacket 19 bottom be provided with spout hole 191, when fluid level sensing apparatus 18 lost efficacy, can drainage overflow liquid, avoid liquid flow into machine intimate.The discharging tube of connection spout hole 191 arranges solenoid valve 14, prevents outside liquid from pouring in down a chimney in protection water jacket 19.
In the present embodiment, light sensing apparatus 17 is reflected light sensing apparatus, its light transmitting terminal and optical receiving end are arranged in the same side (rear side of the micro-color pond 11 of Fig. 1) of micro-color pond 11, principle is: light transmitting terminal utilizing emitted light shines on the sample of slide 20, the reflected light of sample is received by optical receiving end, sample thickness is large, and the light of reflection is weak; Sample thickness is little, the light intensity of reflection.Intensity of reflected light is converted to corresponding electric signal by optical receiving end, is supplied to main control module, shown in composition graphs 2.Certainly, light sensing apparatus 17 also can be transmitted light sensing apparatus, and its light transmitting terminal and optical receiving end lay respectively at the both sides of micro-color pond, by the intensity of transmitted light, and the thickness of judgement sample.
Fluid level sensing apparatus 18 is arranged on the warning liquid level place in micro-color pond 11, and when the super warning height of liquid level, sensing signal is supplied to main control module by fluid level sensing apparatus 18.
Main control module, for controlling the operation of described peristaltic pump, to realize the control of the fluid injection flow to micro-color pond 11, or stops fluid injection when liquid level exceedes warning height in micro-color pond 11; Main control module is also for controlling the dyeing time to this slide according to the sample thickness (and light sensing apparatus 17 provide signal) on slide.
Display and key-press module, its display section is used for display switch machine state and dyeing time, and key part is for controlling startup and the stopping of the dying operation of dyeing instrument.
The flowing exhaust dyeing instrument that above-described embodiment provides, its course of work is as follows:
To the slide of sample be coated, and put into micro-color pond 11, bring into operation by start key.First, the light signal that light sensing apparatus 17 first measures its reflection to the sample on slide is strong and weak, decides with this light signal power the time increasing or reduce dyeing.Afterwards, main control module is given an order, and is rotated inject dyeing liquor A by peristaltic pump, and liquid injection rate IR is controlled by the peristaltic pump preset working time, if liquid level arrives fluid level sensing apparatus 18, then forced peristaltic pump stops action.
Peristaltic pump starts timing dyeing time after stopping, and the time is set time T × a(a is that photoelectric sensor is measured to signal, and convert the coefficient obtained).After time countdown completes, the peristaltic pump being responsible for discharge opeing rotates, and is discharged by the liquid in sample color pond.
Repeat above action below, by the distribution of switching action and management, respectively by washing fluid and the perfusion of other dyeing liquor and emptying, after the action preset all completes, point out and finish the work until all.The slide contaminated is taken out, wipes the unnecessary liquid in the slide back side with filter paper, be placed on basis of microscopic observation.
Above embodiment is only fully open and unrestricted the present invention, for the simple replacement can known without creative work, or the increase and decrease of constructed feature, scope disclosed in the present application should be belonged to.
Claims (10)
1. flow an exhaust dyeing instrument, it is characterized in that, comprising: micro-color pond, for depositing the slide being loaded with sample of dyeing liquor and monolithic, to have liquid injection port and leakage fluid dram; Liquid injection pipe, connects described liquid injection port and dyeing liquor container or washing fluid container; Discharging tube, connects described leakage fluid dram and dyeing instrument is outside; Peristaltic pump, is arranged on described liquid injection pipe, and its control end connects main control module; Dyeing liquor container, for storing dyeing liquor; Washing fluid container, for storing washing fluid; Light sensing apparatus, is arranged on micro-color pond side, for detecting the thickness of the sample that described slide is loaded with, and detection signal is supplied to main control module; Main control module, analyzes the detection signal of described sample thickness, controls dyeing time, and controls the operation of described peristaltic pump; Display and key-press module, its display section is for showing the running state information of described main control module, and its key part is used for described main control module input control signal.
2. flowing exhaust dyeing instrument according to claim 1, is characterized in that, also comprise fluid level sensing apparatus, and be arranged on the warning liquid level place in micro-color pond, the sensing signal output terminal of this fluid level sensing apparatus connects described main control module; Described main control module also controls described peristaltic pump according to the sensing signal of fluid level sensing apparatus.
3. flowing exhaust dyeing instrument according to claim 1, is characterized in that, also comprises overflow protection water jacket, and described micro-color pond is arranged in this overflow protection water jacket, and overflow protection outer slot bottom is provided with spout hole.
4. flowing exhaust dyeing instrument according to claim 3, it is characterized in that, described spout hole is connected with discharging tube, and this discharging tube is provided with solenoid valve.
5. the flowing exhaust dyeing instrument according to claim 1,2,3 or 4, is characterized in that, described discharging tube is provided with peristaltic pump.
6. the flowing exhaust dyeing instrument according to claim 1,2,3 or 4, is characterized in that, described liquid injection pipe, peristaltic pump and dyeing liquor container are more than two.
7. the flowing exhaust dyeing instrument according to claim 1,2,3 or 4, it is characterized in that, described light sensing apparatus is reflected light sensing apparatus, its light transmitting terminal and optical receiving end are positioned at the same side of described micro-color pond.
8. the flowing exhaust dyeing instrument according to claim 1,2,3 or 4, it is characterized in that, described light sensing apparatus is transmitted light sensing apparatus, its light transmitting terminal and optical receiving end lay respectively at the both sides of described micro-color pond.
9. the flowing exhaust dyeing instrument according to claim 1,2,3 or 4, is characterized in that, the length of described micro-color pond × wide × thick is 55 ~ 60mm × 26 ~ 28mm × 3 ~ 5mm.
10. the flowing exhaust dyeing instrument according to claim 1,2,3 or 4, is characterized in that, the liquid injection port be connected with described dyeing liquor container is arranged on the bottom of micro-color pond; The liquid injection port be connected with described washing fluid container is arranged on the side wall upper part of micro-color pond.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310643854.7A CN103674663B (en) | 2013-12-03 | 2013-12-03 | Flowing exhaust dyeing instrument |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310643854.7A CN103674663B (en) | 2013-12-03 | 2013-12-03 | Flowing exhaust dyeing instrument |
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| CN103674663A CN103674663A (en) | 2014-03-26 |
| CN103674663B true CN103674663B (en) | 2016-04-06 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109342156B (en) * | 2018-10-29 | 2021-01-15 | 重庆英特力科技有限公司 | Automatic slice dyeing device |
| CN109444108A (en) * | 2018-12-26 | 2019-03-08 | 中国原子能科学研究院 | Flow cell |
| CN110286022A (en) * | 2019-05-27 | 2019-09-27 | 郑州中普医疗器械有限公司 | Fast constant temperature high definition dyes structural unit and high definition colouring method |
| CN112113822A (en) * | 2019-06-21 | 2020-12-22 | 深圳迈瑞生物医疗电子股份有限公司 | Biological sample dyeing device, push piece dyeing machine and biological sample dyeing method |
| CN111180058B (en) * | 2020-01-07 | 2023-05-12 | 同济大学 | Automatic urinary cell staining efficiency optimization method |
| CN113495028A (en) * | 2020-03-18 | 2021-10-12 | 苏州百源基因技术有限公司 | High-efficient automatic dyeing appearance |
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| EP2009419A1 (en) * | 2007-06-25 | 2008-12-31 | Milestone S.r.l. | Measuring the thickness of organic samples |
| CN102192852A (en) * | 2010-03-11 | 2011-09-21 | 希森美康株式会社 | Smear staining apparatus, smear processing system, and method for determining staining condition |
| CN202649010U (en) * | 2011-12-30 | 2013-01-02 | 天津百利鑫生物科技有限公司 | Settling type automatic smear-making and dyeing machine for liquid-based cell |
| CN103308369A (en) * | 2013-05-07 | 2013-09-18 | 华中农业大学 | Full-automatic silver staining developing instrument |
| CN203798649U (en) * | 2013-12-03 | 2014-08-27 | 珠海贝索生物技术有限公司 | Flow dip dyeing apparatus |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE202007010593U1 (en) * | 2007-07-27 | 2007-09-27 | Leica Mikrosysteme Gmbh | Contrasting device for sample cuts |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2009419A1 (en) * | 2007-06-25 | 2008-12-31 | Milestone S.r.l. | Measuring the thickness of organic samples |
| CN102192852A (en) * | 2010-03-11 | 2011-09-21 | 希森美康株式会社 | Smear staining apparatus, smear processing system, and method for determining staining condition |
| CN202649010U (en) * | 2011-12-30 | 2013-01-02 | 天津百利鑫生物科技有限公司 | Settling type automatic smear-making and dyeing machine for liquid-based cell |
| CN103308369A (en) * | 2013-05-07 | 2013-09-18 | 华中农业大学 | Full-automatic silver staining developing instrument |
| CN203798649U (en) * | 2013-12-03 | 2014-08-27 | 珠海贝索生物技术有限公司 | Flow dip dyeing apparatus |
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